pycom02g07550
ERF Family

Cupin superfamily (DUF985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013260
Physical Location & Seq
Reverse (-)
5275075 .. 5276730
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGGGAACTGCATCAGAGATTGTAGCTAAACTGAAACTGAATCCCCACCCTGAAGGTGGCTTTTATTTCGAAACTTTCAGAGATACTTCCATTGCCCTGCCTTTGTGTCAGCTTCCCCCAGACTACAAGGTTGAGCGCCCTGTGAGTTCATCTATCTACTTCTTGCTGCCGTCTGGGAGTGTATCTCGCCTTCATCGTATACCAATGGCAGAAACCTGGCATTTTTATGTCGGAGAACCCATTATGATCGTGGAGTTGGGTGAAGATGGGAAAGTCAAGTTAACCTGCCTCGGACCCGATTTGGGAGCGGATCAGCAACCCCAGTACACAGTGCCTCCAAATGTATGGTTTGGTTCCTTCCCAACAAAGGACCACAGCATTTCACCAGAAGGGGCATTGCTCAAAGCTGCTCCAAGGGATGCCGAGACTCACTACTCTCTCGTGGGATGTACTTGTGCACCTGCCTTTCAGTTCCAGGATTTCGAGTTGGCAAAACGCTCCGATCGATTTCTTCCCCTTTTTCCTGGTTACGAGGCTCTCATAACGCTGCTTACTTTCCCTGAAGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_5

Protein Analysis

191

Amino Acids

21.09

Weight (kDa)

5.07

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_5 PF06172 6 - 164 1.2e-43 Cupin superfamily (DUF985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19130
fragaria_vesca FvH4_1g09020
malus_domestica MD02G1095400.v1.1
prunus_persica Prupe.7G195700_v2.0.a1
pyrus_communis pycom02g07550
rosa_chinensis RchiOBHm_Chr2g0095441
rosa_laevigata RLG00000016566
rosa_multiflora Rmu_co8132630.1_g000001 Rmu_co8397589.1_g000001 Rmu_sc0010847.1_g000003 Rmu_ssc0000183.1_g000046
rosa_roxburghii Rroxscaffold_2G00146370
rosa_rugosa Rorug02G0051100
rosa_samantha Rh2AG099100 Rh2BG100300 Rh2CG102500 Rh2DG100000
rosa_wichuraiana Rw2G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 2 cut(s) 293, 469
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 199
AciI CCGC 1 cut(s) 308
AclWI GGATC 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 2 cut(s) 326, 451
AfiI CCNNNNNNNGG 3 cut(s) 56, 302, 367
AjnI CCWGG 3 cut(s) 215, 474, 523
AluBI AGCT 3 cut(s) 26, 112, 407
AluI AGCT 3 cut(s) 26, 112, 407
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 419
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 1 cut(s) 318
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 166, 407, 547
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 2 cut(s) 293, 370
AsuHPI GGTGA 2 cut(s) 272, 375
AsuII TTCGAA 1 cut(s) 69
AvaII GGWCC 2 cut(s) 293, 370
BaeGI GKGCMC 1 cut(s) 460
BarI GAAGNNNNNNTAC 2 cut(s) 174, 206
BauI CACGAG 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 3 cut(s) 153, 394, 534
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 3 cut(s) 217, 476, 525
BcoDI GTCTC 1 cut(s) 419
BfoI RGCGCY 1 cut(s) 139
BfuAI ACCTGC 2 cut(s) 293, 469
BisI GCNGC 3 cut(s) 167, 408, 548
BlsI GCNGC 3 cut(s) 168, 409, 549
Bme1390I CCNGG 3 cut(s) 217, 476, 525
Bme18I GGWCC 2 cut(s) 293, 370
BmgT120I GGNCC 2 cut(s) 293, 370
BmiI GGNNCC 2 cut(s) 295, 355
BmrFI CCNGG 3 cut(s) 217, 476, 525
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 2 cut(s) 20, 409
BmuI ACTGGG 1 cut(s) 316
BplI GAGNNNNNCTC 2 cut(s) 169, 201
Bpu14I TTCGAA 1 cut(s) 69
Bsa29I ATCGAT 1 cut(s) 505
BsaJI CCNNGG 2 cut(s) 289, 413
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 302, 367
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 2 cut(s) 90, 395
BseBI CCWGG 3 cut(s) 217, 476, 525
BseCI ATCGAT 1 cut(s) 505
BseDI CCNNGG 2 cut(s) 289, 413
BseGI GGATG 2 cut(s) 424, 452
BseLI CCNNNNNNNGG 3 cut(s) 56, 302, 367
BseMI GCAATG 2 cut(s) 90, 395
BseNI ACTGG 1 cut(s) 322
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 3 cut(s) 153, 394, 534
Bsh1285I CGRYCG 1 cut(s) 505
BshVI ATCGAT 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 505
BsiHKAI GWGCWC 1 cut(s) 460
BslI CCNNNNNNNGG 3 cut(s) 56, 302, 367
BsmAI GTCTC 1 cut(s) 419
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 3 cut(s) 246, 310, 502
BspACI CCGC 1 cut(s) 308
BspDI ATCGAT 1 cut(s) 505
BspLI GGNNCC 2 cut(s) 295, 355
BspMI ACCTGC 2 cut(s) 293, 469
BspPI GGATC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 69
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 90, 395
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 289, 413
BssMI GATC 3 cut(s) 246, 310, 502
BssNAI GTATAC 1 cut(s) 200
BssSI CACGAG 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 413
Bst1107I GTATAC 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 3 cut(s) 217, 476, 525
Bst4CI ACNGT 1 cut(s) 331
BstBI TTCGAA 1 cut(s) 69
BstF5I GGATG 2 cut(s) 424, 452
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 3 cut(s) 249, 313, 505
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 3 cut(s) 246, 310, 502
BstMCI CGRYCG 1 cut(s) 505
BstNI CCWGG 3 cut(s) 217, 476, 525
BstSCI CCNGG 3 cut(s) 215, 474, 523
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 153, 394, 534
BstXI CCANNNNNNTGG 1 cut(s) 345
BstZ17I GTATAC 1 cut(s) 200
Bsu15I ATCGAT 1 cut(s) 505
BsuTUI ATCGAT 1 cut(s) 505
BtsCI GGATG 2 cut(s) 424, 452
BtsIMutI CAGTG 1 cut(s) 336
BveI ACCTGC 2 cut(s) 293, 469
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 2 cut(s) 293, 370
ClaI ATCGAT 1 cut(s) 505
Csp6I GTAC 2 cut(s) 325, 450
CviJI RGCY 5 cut(s) 26, 60, 112, 407, 536
CviKI_1 RGCY 5 cut(s) 26, 60, 112, 407, 536
CviQI GTAC 2 cut(s) 325, 450
DpnI GATC 3 cut(s) 248, 312, 504
DpnII GATC 3 cut(s) 246, 310, 502
Eco130I CCWWGG 1 cut(s) 413
Eco47I GGWCC 2 cut(s) 293, 370
Eco57I CTGAAG 1 cut(s) 72
EcoRII CCWGG 3 cut(s) 215, 474, 523
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaiI YATR 6 cut(s) 200, 228, 245, 346, 542, 571
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 3 cut(s) 167, 408, 548
FokI GGATG 2 cut(s) 431, 459
Fsp4HI GCNGC 3 cut(s) 167, 408, 548
GlaI GCGC 1 cut(s) 137
GluI GCNGC 3 cut(s) 167, 408, 548
HaeII RGCGCY 1 cut(s) 139
HhaI GCGC 1 cut(s) 138
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 2 cut(s) 40, 427
HpaI GTTAAC 1 cut(s) 282
HphI GGTGA 2 cut(s) 272, 375
Hpy166II GTNNAC 4 cut(s) 200, 282, 327, 458
Hpy188I TCNGA 5 cut(s) 16, 80, 233, 293, 502
Hpy8I GTNNAC 4 cut(s) 200, 282, 327, 458
HpyAV CCTTC 4 cut(s) 47, 200, 367, 383
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4V TGCA 2 cut(s) 11, 458
HspAI GCGC 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 282
Kzo9I GATC 3 cut(s) 246, 310, 502
LmnI GCTCC 3 cut(s) 305, 415, 503
Lsp1109I GCAGC 3 cut(s) 153, 394, 534
LweI GCATC 2 cut(s) 20, 409
MaeIII GTNAC 1 cut(s) 527
MalI GATC 3 cut(s) 248, 312, 504
MbiI CCGCTC 1 cut(s) 308
MboI GATC 3 cut(s) 246, 310, 502
MboII GAAGA 2 cut(s) 275, 503
MhlI GDGCHC 1 cut(s) 460
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 3 cut(s) 299, 345, 526
MseI TTAA 1 cut(s) 281
MslI CAYNNNNRTG 1 cut(s) 225
MspR9I CCNGG 3 cut(s) 217, 476, 525
MvaI CCWGG 3 cut(s) 217, 476, 525
NdeII GATC 3 cut(s) 246, 310, 502
NlaIV GGNNCC 2 cut(s) 295, 355
NmeAIII GCCGAG 1 cut(s) 448
NspV TTCGAA 1 cut(s) 69
PaqCI CACCTGC 1 cut(s) 469
PcsI WCGNNNNNNNCGW 2 cut(s) 193, 502
PfeI GAWTC 1 cut(s) 40
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 3 cut(s) 168, 409, 549
Ple19I CGATCG 1 cut(s) 505
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Psp6I CCWGG 3 cut(s) 215, 474, 523
PspGI CCWGG 3 cut(s) 215, 474, 523
PspN4I GGNNCC 2 cut(s) 295, 355
PspPI GGNCC 2 cut(s) 293, 370
PvuI CGATCG 1 cut(s) 505
RsaI GTAC 2 cut(s) 326, 451
RsaNI GTAC 2 cut(s) 325, 450
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 3 cut(s) 167, 408, 548
Sau3AI GATC 3 cut(s) 246, 310, 502
Sau96I GGNCC 2 cut(s) 293, 370
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 3 cut(s) 217, 476, 525
SduI GDGCHC 1 cut(s) 460
SetI ASST 8 cut(s) 28, 58, 114, 132, 218, 287, 409, 463
SfaNI GCATC 2 cut(s) 20, 409
SfuI TTCGAA 1 cut(s) 69
SinI GGWCC 2 cut(s) 293, 370
SmiMI CAYNNNNRTG 1 cut(s) 225
SsiI CCGC 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 215, 474, 523
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 1 cut(s) 331
TaqI TCGA 3 cut(s) 69, 483, 505
TatI WGTACW 2 cut(s) 324, 449
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 1 cut(s) 336
TseI GCWGC 3 cut(s) 166, 407, 547
TspDTI ATGAA 2 cut(s) 138, 182
TspRI CASTG 1 cut(s) 336
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 2 cut(s) 293, 370
XcmI CCANNNNNNNNNTGG 2 cut(s) 53, 247
XmiI GTMKAC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.