pycom02g13810

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012566
Physical Location & Seq
Reverse (-)
10397366 .. 10400326
2961 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13810.1

Sequence Viewer

Length: 2961 bp
ATGAGTTTCTTCCTCCGACACTCCAATACTCTATCTTCTTCCCCGTTGATTCTCTCTCTTGTCAGACACAAACATTCTTCAATCAATTCGAGAACACCTTGCTTCCTCCGTCTGCTAAACCTCTGCAAAACCTGCGAGGACTCGAGGCCTCTCACATCCCTTCTCATCGTACAAGGACTTGTTAAGGATGTTTTTCTGGTGGAGCAATTCGTCAGGTCCTGTTTTCATCTGGGTTCTTCACATTTAGCTCTCTCTCTATTCCAACAAATCCAAAGGCCGAGTCTTGGCCTGCAGAACCTCGTAATCCGGTACCTTAGCAATCACGGCTTATACCATGAGCTCTTGCATGTCTACCTCCATTCCGGGGCTTCCGGTTGCTCTTCTGATGACTTTACGTTTCCATTCGTCATCAAGGCGTGCGCGGCGCTGGCTGCGATTAGGACTGGGAAAGAGGTTCATGGAGTCGTGATAAGAACTGGGTTTGAGCGAAACCTTGTTATACAGACTGCATTGGTTGATTTCTATGCGAAAACCGGTTGTATGGGGATGGCACGTCAACTGATTGATAGAATTCCTCAACCAGACTTGGTTGCTTGGAATGCACTCATTGCTGGTTATTCGTTGAACGGATTGAATTGGGAAGCGTTTGATGTTTTTGAGGAGATTCTCGTTATGGATTTGAAGCCAAATTTGAGCACTCTGGCCACTGTTATTCCTGTTTGCACTCGCTTGGGGTGTATGCATACCGGTAAGTCCCTACATTGCTTTGCTATCAAATCTGGTTACTTGTCGAATAATTTTTTGGTTCCTGCTTTGATTTCAATGTATGCCGGGGATGAGGATTTGTGCGGTGCTAGAAACTTGTTTGATTCTGTACCGGAAAAGAATGTTGTTGTTTGGAATGCTATGATTTCTGCATACACACAAATGCAGAGGGCCATCTCAGCTTTTGAGATGTTCCGTTGTATGCTTCGGGTTGACTTGCAGCCTAATTTCGTCACTTTCGTGTCCGTTATTCCCTCATGTGAGAATTCCAGTAGCCTTTCTTTCGGCGAGTCCCTTCATGCTTGTGTTAATAAACATGGATCAGAAAATCAACTGCCAGTCCTAACAGCCCTTGTATCAATGTATGCCAAGCTTGGAGACACTGATTCATCCCGGTATCTTTTCAAGCCAATGCCCAGTAAAAACCTTCTTTTGTGGAACTCAATGATTTCTGGGTACGTGTACAACGGGCTATGGAATTTAAGTCTCGGTGTATTTCGTGAAATGCAATTTTCAGGGTCTGTTCCAGATGCAGTCTCCATCGTTAGTGTTCTTTCTGCGTGCTCCAAACTTGAGGCGGATTTAGTGGGGAAGTCTGTGCATGCATTTAGTATTAGGAAGGGTTTCCTCTCAAATCTCAATCTGTCAAATGCCCTGTTGGCATTTTACTCCGATTGTCATCGCCTCTCCTATTCAATTACTTTGTTGCGTGAAATGCCTACAAAAAATTCCATAACATGGAACACGTTAATATCCTGTTGTGTGCACCACTGCGAGATGGAAAAGTCGGTTGCCTTTTACTACCAGATGCAGAAAGAGGGCTTCCAGTTGGACTTAGTCACCCTGATAAGCATACTCCCTAGTCTCAGTGAGAAGAAAAGCTTAGGGCAAGGAATGGCTATTCATTGTTACACTATAAAATACGGGTTCTCTGCCGACATTTCTCTGGCAAATTCACTTATTAGCATGTACTGCAACTGTGGAGACCTTGATGCCGGGAGGTTACTTTTCAAAGTCATGCCAACGAGGAGTGTGGTGTCTTGGAATGCTCTGATGACTGGCTTCCGAAACCACGATTTACAGAATGAGGTTTGGGTCTTGTTTGGTGAAATGATAAAGGATGATGAGAGACCAAATTATGTAACTTTGCTAAATTTATTGCCAGCATGCCGCACACAATTGCAGGGTAAGTCTATCCATGCTGTCGCCTTCAGAACCGGAATTGTACAAGAAACCCCTCTCCTTACATCACTTATGTTCATGTATGCTAGATTTGAGAACATTAACTCTTGCCTGTTACAGTTCCAAACGGGAAACATGAAGGATATTTCGTTGTGGAATGCGTTTTTGTCTGTGCAGATACAAACTAAAAATAGCGAAATTGCAGTTTCATTTTTCCGCGGATTGCTTCGGATGGGATTGGAACCCGACAATGTGACAGTTCTGAGTTTAGTTTCTGCTTGTGTTCAACTCAATAGCCTCAATCTTGCAGATTGTGTAACGGCGTACATAATACGAAAAGGCCTTGACAAAGATCTGGTTATCAGCAATGCCCTAATCAATATGCTTGCAACATGCGGAAACATATTGAGTGCAAGGAAGCTGTTTGAAGGGCTGGTTGAAAAGGATTCCGTGTCTTGGAATGTGATGATTAATGGATACGGTCTGCACGGGGACGGTGAAGGTGCCCTAGATCTTTTCTTGAAGATGAAGCTTTCCGGAGTAAAACCTAATGGGATTACATATTCAAGTATTCTATCAGCCTGCAGCCATTGCGGCTTGGTCGAGCAAGGCCGGTTGGTATTCAACTCTATGGCGGAACATGGGATATCACCCGAAATCGAGCATTATGCCTGCATGGTGGATCTTCTCGGAAGAACAGGTAACCTGACTGAGGCTTATGGCATTGTTAGGGCACTGCCCTTTAAACCTTCAACAAGTATGCTTGAATCTTTGTTGGGGGCCTGTAGAATCCATGAGGATGTTGAACTTGGCGAAAGAATTGGTAGGATGCTTTCGGAATCGGACCCGGAAAACTCGAGGTCACATGTGATGCTTTACAATGTGTATGCAGCAGCTGGGAGGTGGGGAGATGCTGAGAGAGTGAGGTCTGAGATGGAAGAAAGACAACTGAGAAAAGTACCTGGATTTAGTCTTGTCATGGGAAATGGATTCAATGATAAGGAAGTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 WEMBL

Protein Analysis

987

Amino Acids

109.52

Weight (kDa)

7.1

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 194 - 240 6.1e-06 PPR repeat family
PPR_2 PF13041 295 - 342 5e-07 PPR repeat family
PPR_2 PF13041 398 - 445 6.9e-06 PPR repeat family
PPR_2 PF13041 497 - 541 4.4e-07 PPR repeat family
PPR_2 PF13041 797 - 845 1.5e-13 PPR repeat family
PPR PF01535 800 - 830 4.4e-08 PPR repeat
E_motif PF20431 914 - 975 1.2e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 140
Acc65I GGTACC 1 cut(s) 309
AccB1I GGYRCC 2 cut(s) 309, 2450
AccB7I CCANNNNNTGG 1 cut(s) 1503
AccI GTMKAC 1 cut(s) 351
AccII CGCG 2 cut(s) 422, 2166
AccIII TCCGGA 1 cut(s) 2483
AciI CCGC 9 cut(s) 422, 849, 1343, 1936, 2164, 2166, 2343, 2541, 2582
AclWI GGATC 2 cut(s) 1093, 2637
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 7 cut(s) 570, 688, 1030, 1243, 1492, 1717, 1918
AcuI CTGAAG 1 cut(s) 1960
AfaI GTAC 9 cut(s) 171, 311, 876, 1223, 1229, 1736, 1992, 2273, 2907
AfiI CCNNNNNNNGG 5 cut(s) 284, 364, 1503, 2403, 2659
AflIII ACRYGT 3 cut(s) 1224, 1509, 2812
AgeI ACCGGT 2 cut(s) 533, 746
AjiI CACGTC 1 cut(s) 554
AjnI CCWGG 1 cut(s) 2908
AjuI GAANNNNNNNTTGG 2 cut(s) 785, 817
AleI CACNNNNGTG 1 cut(s) 1004
AluBI AGCT 8 cut(s) 248, 340, 947, 1138, 1647, 2368, 2479, 2843
AluI AGCT 8 cut(s) 248, 340, 947, 1138, 1647, 2368, 2479, 2843
Alw21I GWGCWC 4 cut(s) 342, 698, 1331, 1533
Alw26I GTCTC 6 cut(s) 1137, 1256, 1306, 1634, 1743, 1888
Alw44I GTGCAC 1 cut(s) 1529
AlwI GGATC 2 cut(s) 1093, 2637
AlwNI CAGNNNCTG 3 cut(s) 219, 1286, 2843
Ama87I CYCGRG 2 cut(s) 142, 2803
Aor13HI TCCGGA 1 cut(s) 2483
AoxI GGCC 8 cut(s) 146, 275, 286, 702, 936, 2287, 2557, 2727
ApaLI GTGCAC 1 cut(s) 1529
ApeKI GCWGC 5 cut(s) 431, 985, 2532, 2837, 2840
ApoI RAATTY 7 cut(s) 570, 688, 1030, 1243, 1492, 1717, 1918
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
AseI ATTAAT 1 cut(s) 2418
AsiGI ACCGGT 2 cut(s) 533, 746
Asp700I GAANNNNTTC 1 cut(s) 1388
Asp718I GGTACC 1 cut(s) 309
AspLEI GCGC 2 cut(s) 422, 427
AspS9I GGNCC 4 cut(s) 216, 936, 2727, 2791
AsuC2I CCSGG 5 cut(s) 364, 832, 1159, 1762, 2795
AsuHPI GGTGA 4 cut(s) 1597, 1883, 2456, 2589
AvaI CYCGRG 2 cut(s) 142, 2803
AvaII GGWCC 2 cut(s) 216, 2791
BaeGI GKGCMC 3 cut(s) 1533, 2455, 2683
BalI TGGCCA 1 cut(s) 704
BanI GGYRCC 2 cut(s) 309, 2450
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 4 cut(s) 342, 698, 1331, 1533
BbvI GCAGC 5 cut(s) 418, 997, 2544, 2849, 2852
BccI CCATC 6 cut(s) 541, 947, 1313, 1537, 2173, 2875
BceAI ACGGC 2 cut(s) 340, 2283
BcgI CGANNNNNNTGC 2 cut(s) 2597, 2631
BciT130I CCWGG 1 cut(s) 2910
BciVI GTATCC 1 cut(s) 2417
BcnI CCSGG 5 cut(s) 364, 832, 1159, 1762, 2795
BcoDI GTCTC 6 cut(s) 1137, 1256, 1306, 1634, 1743, 1888
BfaI CTAG 4 cut(s) 855, 1626, 2034, 2456
BfmI CTRYAG 3 cut(s) 290, 2530, 2731
BfoI RGCGCY 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 140
BfuI GTATCC 1 cut(s) 2417
BglI GCCNNNNNGGC 2 cut(s) 1424, 2541
BglII AGATCT 2 cut(s) 2299, 2458
BisI GCNGC 8 cut(s) 423, 432, 986, 1936, 2533, 2542, 2838, 2841
BlsI GCNGC 8 cut(s) 424, 433, 987, 1937, 2534, 2543, 2839, 2842
Bme1390I CCNGG 6 cut(s) 364, 832, 1159, 1762, 2795, 2910
Bme18I GGWCC 2 cut(s) 216, 2791
BmeT110I CYCGRG 2 cut(s) 142, 2803
BmgBI CACGTC 1 cut(s) 554
BmgT120I GGNCC 4 cut(s) 216, 936, 2727, 2791
BmiI GGNNCC 6 cut(s) 311, 807, 2190, 2452, 2728, 2793
BmrFI CCNGG 6 cut(s) 364, 832, 1159, 1762, 2795, 2910
BmrI ACTGGG 3 cut(s) 453, 486, 1176
BmsI GCATC 6 cut(s) 1285, 1563, 1747, 2766, 2808, 2848
BmuI ACTGGG 3 cut(s) 453, 486, 1176
Bpu10I CCTNAGC 2 cut(s) 314, 1648
BpuEI CTTGAG 1 cut(s) 1358
BpuMI CCSGG 5 cut(s) 364, 832, 1159, 1762, 2795
BsaAI YACGTR 1 cut(s) 1225
BsaBI GATNNNNATC 1 cut(s) 1443
BsaI GGTCTC 2 cut(s) 1743, 1888
BsaJI CCNNGG 3 cut(s) 363, 831, 2164
BsaWI WCCGGW 7 cut(s) 306, 371, 533, 746, 877, 1982, 2483
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 364, 1503, 2403, 2659
Bse118I RCCGGY 3 cut(s) 533, 746, 2559
Bse1I ACTGG 7 cut(s) 448, 481, 1035, 1103, 1182, 1591, 1828
Bse3DI GCAATG 4 cut(s) 606, 760, 2320, 2536
Bse8I GATNNNNATC 1 cut(s) 1443
BseAI TCCGGA 1 cut(s) 2483
BseBI CCWGG 1 cut(s) 2910
BseDI CCNNGG 3 cut(s) 363, 831, 2164
BseGI GGATG 9 cut(s) 155, 193, 552, 841, 1154, 1891, 2184, 2752, 2781
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 5 cut(s) 284, 364, 1503, 2403, 2659
BseMI GCAATG 4 cut(s) 606, 760, 2320, 2536
BseMII CTCAG 7 cut(s) 957, 1645, 2201, 2649, 2853, 2868, 2888
BseNI ACTGG 7 cut(s) 448, 481, 1035, 1103, 1182, 1591, 1828
BseRI GAGGAG 2 cut(s) 674, 1807
BseSI GKGCMC 3 cut(s) 1533, 2455, 2683
BseXI GCAGC 5 cut(s) 418, 997, 2544, 2849, 2852
BseYI CCCAGC 1 cut(s) 2843
BsgI GTGCAG 2 cut(s) 2141, 2417
Bsh1236I CGCG 2 cut(s) 422, 2166
BshFI GGCC 8 cut(s) 148, 277, 288, 704, 938, 2289, 2559, 2729
BshNI GGYRCC 2 cut(s) 309, 2450
BshTI ACCGGT 2 cut(s) 533, 746
BsiHKAI GWGCWC 4 cut(s) 342, 698, 1331, 1533
BsiHKCI CYCGRG 2 cut(s) 142, 2803
BslFI GGGAC 3 cut(s) 739, 1042, 2453
BslI CCNNNNNNNGG 5 cut(s) 284, 364, 1503, 2403, 2659
BsmAI GTCTC 6 cut(s) 1137, 1256, 1306, 1634, 1743, 1888
BsmFI GGGAC 3 cut(s) 739, 1042, 2453
BsmI GAATGC 4 cut(s) 604, 907, 1816, 2110
BsnI GGCC 8 cut(s) 148, 277, 288, 704, 938, 2289, 2559, 2729
Bso31I GGTCTC 2 cut(s) 1743, 1888
BsoBI CYCGRG 2 cut(s) 142, 2803
Bsp1286I GDGCHC 6 cut(s) 342, 698, 1331, 1533, 2455, 2683
Bsp13I TCCGGA 1 cut(s) 2483
Bsp1407I TGTACA 2 cut(s) 1227, 1990
Bsp143I GATC 4 cut(s) 1085, 2299, 2458, 2629
BspACI CCGC 9 cut(s) 422, 849, 1343, 1936, 2164, 2166, 2343, 2541, 2582
BspANI GGCC 8 cut(s) 148, 277, 288, 704, 938, 2289, 2559, 2729
BspCNI CTCAG 7 cut(s) 956, 1644, 2202, 2650, 2854, 2869, 2889
BspEI TCCGGA 1 cut(s) 2483
BspFNI CGCG 2 cut(s) 422, 2166
BspLI GGNNCC 6 cut(s) 311, 807, 2190, 2452, 2728, 2793
BspMAI CTGCAG 2 cut(s) 294, 2534
BspMI ACCTGC 1 cut(s) 140
BspPI GGATC 2 cut(s) 1093, 2637
BspQI GCTCTTC 1 cut(s) 385
BspT107I GGYRCC 2 cut(s) 309, 2450
BspTNI GGTCTC 2 cut(s) 1743, 1888
BsrDI GCAATG 4 cut(s) 606, 760, 2320, 2536
BsrFI RCCGGY 3 cut(s) 533, 746, 2559
BsrGI TGTACA 2 cut(s) 1227, 1990
BsrI ACTGG 7 cut(s) 448, 481, 1035, 1103, 1182, 1591, 1828
BssAI RCCGGY 3 cut(s) 533, 746, 2559
BssECI CCNNGG 3 cut(s) 363, 831, 2164
BssMI GATC 4 cut(s) 1085, 2299, 2458, 2629
Bst2UI CCWGG 1 cut(s) 2910
Bst4CI ACNGT 6 cut(s) 709, 1745, 2067, 2206, 2429, 2444
Bst6I CTCTTC 1 cut(s) 385
BstAPI GCANNNNNTGC 4 cut(s) 132, 608, 1737, 2538
BstAUI TGTACA 2 cut(s) 1227, 1990
BstBAI YACGTR 1 cut(s) 1225
BstDSI CCRYGG 1 cut(s) 2164
BstEII GGTNACC 1 cut(s) 2648
BstENI CCTNNNNNAGG 1 cut(s) 2657
BstF5I GGATG 9 cut(s) 155, 193, 552, 841, 1154, 1891, 2184, 2752, 2781
BstFNI CGCG 2 cut(s) 422, 2166
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 2 cut(s) 422, 427
BstKTI GATC 4 cut(s) 1088, 2302, 2461, 2632
BstMAI GTCTC 6 cut(s) 1137, 1256, 1306, 1634, 1743, 1888
BstMBI GATC 4 cut(s) 1085, 2299, 2458, 2629
BstNI CCWGG 1 cut(s) 2910
BstNSI RCATGY 6 cut(s) 350, 1370, 1735, 1935, 2343, 2816
BstPI GGTNACC 1 cut(s) 2648
BstSCI CCNGG 6 cut(s) 362, 830, 1157, 1760, 2793, 2908
BstSFI CTRYAG 3 cut(s) 290, 2530, 2731
BstSLI GKGCMC 3 cut(s) 1533, 2455, 2683
BstUI CGCG 2 cut(s) 422, 2166
BstV1I GCAGC 5 cut(s) 418, 997, 2544, 2849, 2852
BstX2I RGATCY 3 cut(s) 2299, 2458, 2629
BstYI RGATCY 3 cut(s) 2299, 2458, 2629
BsuI GTATCC 1 cut(s) 2417
BsuRI GGCC 8 cut(s) 148, 277, 288, 704, 938, 2289, 2559, 2729
BtgI CCRYGG 1 cut(s) 2164
BtgZI GCGATG 1 cut(s) 1430
BtrI CACGTC 1 cut(s) 554
BtsCI GGATG 9 cut(s) 155, 193, 552, 841, 1154, 1891, 2184, 2752, 2781
BtsI GCAGTG 2 cut(s) 1534, 2681
BtsIMutI CAGTG 5 cut(s) 705, 1146, 1534, 1639, 2681
BveI ACCTGC 1 cut(s) 140
CaiI CAGNNNCTG 3 cut(s) 219, 1286, 2843
CfoI GCGC 2 cut(s) 422, 427
Cfr10I RCCGGY 3 cut(s) 533, 746, 2559
Cfr13I GGNCC 4 cut(s) 216, 936, 2727, 2791
Cfr42I CCGCGG 1 cut(s) 2167
Csp6I GTAC 9 cut(s) 170, 310, 875, 1222, 1228, 1735, 1991, 2272, 2906
CspAI ACCGGT 2 cut(s) 533, 746
CviQI GTAC 9 cut(s) 170, 310, 875, 1222, 1228, 1735, 1991, 2272, 2906
DpnI GATC 4 cut(s) 1087, 2301, 2460, 2631
DpnII GATC 4 cut(s) 1085, 2299, 2458, 2629
DraI TTTAAA 1 cut(s) 2692
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
EciI GGCGGA 2 cut(s) 1358, 2597
Ecl136II GAGCTC 1 cut(s) 340
Eco147I AGGCCT 2 cut(s) 148, 2289
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 2 cut(s) 1743, 1888
Eco32I GATATC 1 cut(s) 2595
Eco47I GGWCC 2 cut(s) 216, 2791
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 1960
Eco88I CYCGRG 2 cut(s) 142, 2803
Eco91I GGTNACC 1 cut(s) 2648
EcoICRI GAGCTC 1 cut(s) 340
EcoNI CCTNNNNNAGG 1 cut(s) 2657
EcoO109I RGGNCCY 2 cut(s) 216, 2727
EcoO65I GGTNACC 1 cut(s) 2648
EcoRI GAATTC 2 cut(s) 570, 1030
EcoRII CCWGG 1 cut(s) 2908
EcoRV GATATC 1 cut(s) 2595
EcoT22I ATGCAT 2 cut(s) 744, 1372
EcoT38I GRGCYC 1 cut(s) 342
FalI AAGNNNNNCTT 2 cut(s) 1631, 1663
FaqI GGGAC 3 cut(s) 739, 1042, 2453
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 8 cut(s) 423, 432, 986, 1936, 2533, 2542, 2838, 2841
FokI GGATG 9 cut(s) 142, 200, 559, 848, 1141, 1898, 2191, 2759, 2788
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 8 cut(s) 423, 432, 986, 1936, 2533, 2542, 2838, 2841
FspBI CTAG 4 cut(s) 855, 1626, 2034, 2456
GlaI GCGC 2 cut(s) 421, 426
GluI GCNGC 8 cut(s) 423, 432, 986, 1936, 2533, 2542, 2838, 2841
GsaI CCCAGC 1 cut(s) 2847
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 8 cut(s) 148, 277, 288, 704, 938, 2289, 2559, 2729
HhaI GCGC 2 cut(s) 422, 427
Hin6I GCGC 2 cut(s) 420, 425
HinP1I GCGC 2 cut(s) 420, 425
HincII GTYRAC 2 cut(s) 557, 979
HindII GTYRAC 2 cut(s) 557, 979
HindIII AAGCTT 3 cut(s) 1136, 1645, 2477
HphI GGTGA 4 cut(s) 1597, 1883, 2456, 2589
Hpy166II GTNNAC 5 cut(s) 352, 557, 979, 1228, 1531
Hpy188III TCNNGA 6 cut(s) 90, 466, 1265, 1292, 2467, 2484
Hpy8I GTNNAC 5 cut(s) 352, 557, 979, 1228, 1531
HpyAV CCTTC 9 cut(s) 170, 1070, 1202, 1378, 1984, 2080, 2369, 2441, 2706
HpyCH4III ACNGT 6 cut(s) 709, 1745, 2067, 2206, 2429, 2444
HpyCH4IV ACGT 4 cut(s) 395, 553, 1224, 1511
HpySE526I ACGT 4 cut(s) 395, 553, 1224, 1511
HspAI GCGC 2 cut(s) 420, 425
Kpn2I TCCGGA 1 cut(s) 2483
KpnI GGTACC 1 cut(s) 313
KspI CCGCGG 1 cut(s) 2167
Kzo9I GATC 4 cut(s) 1085, 2299, 2458, 2629
LguI GCTCTTC 1 cut(s) 385
LmnI GCTCC 2 cut(s) 202, 1334
Lsp1109I GCAGC 5 cut(s) 418, 997, 2544, 2849, 2852
LweI GCATC 6 cut(s) 1285, 1563, 1747, 2766, 2808, 2848
MaeI CTAG 4 cut(s) 855, 1626, 2034, 2456
MaeII ACGT 4 cut(s) 395, 553, 1224, 1511
MalI GATC 4 cut(s) 1087, 2301, 2460, 2631
MboI GATC 4 cut(s) 1085, 2299, 2458, 2629
MfeI CAATTG 1 cut(s) 1943
MflI RGATCY 3 cut(s) 2299, 2458, 2629
MhlI GDGCHC 6 cut(s) 342, 698, 1331, 1533, 2455, 2683
MlsI TGGCCA 1 cut(s) 704
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 4 cut(s) 134, 289, 471, 1064
MmeI TCCRAC 3 cut(s) 40, 286, 1575
Mox20I TGGCCA 1 cut(s) 704
Mph1103I ATGCAT 2 cut(s) 744, 1372
MroI TCCGGA 1 cut(s) 2483
MroXI GAANNNNTTC 1 cut(s) 1388
MscI TGGCCA 1 cut(s) 704
MseI TTAA 7 cut(s) 183, 1074, 1247, 1514, 2049, 2418, 2691
MslI CAYNNNNRTG 4 cut(s) 926, 1004, 1068, 2745
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 2 cut(s) 2166, 2843
MspR9I CCNGG 6 cut(s) 364, 832, 1159, 1762, 2795, 2910
MteI GCGCNGCGC 1 cut(s) 423
MunI CAATTG 1 cut(s) 1943
Mva1269I GAATGC 4 cut(s) 604, 907, 1816, 2110
MvaI CCWGG 1 cut(s) 2910
MvnI CGCG 2 cut(s) 422, 2166
NciI CCSGG 5 cut(s) 364, 832, 1159, 1762, 2795
NdeII GATC 4 cut(s) 1085, 2299, 2458, 2629
NlaIV GGNNCC 6 cut(s) 311, 807, 2190, 2452, 2728, 2793
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 4 cut(s) 997, 1603, 2200, 2808
NsiI ATGCAT 2 cut(s) 744, 1372
NspI RCATGY 6 cut(s) 350, 1370, 1735, 1935, 2343, 2816
OliI CACNNNNGTG 1 cut(s) 1004
PaeI GCATGC 2 cut(s) 1370, 1935
PaeR7I CTCGAG 2 cut(s) 142, 2803
PceI AGGCCT 2 cut(s) 148, 2289
PciI ACATGT 1 cut(s) 2812
PciSI GCTCTTC 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 1002
PctI GAATGC 4 cut(s) 604, 907, 1816, 2110
PdmI GAANNNNTTC 1 cut(s) 1388
PfeI GAWTC 9 cut(s) 49, 664, 869, 1151, 2393, 2714, 2736, 2786, 2937
PflFI GACNNNGTC 1 cut(s) 1601
PflMI CCANNNNNTGG 1 cut(s) 1503
PinAI ACCGGT 2 cut(s) 533, 746
PkrI GCNGC 8 cut(s) 424, 433, 987, 1937, 2534, 2543, 2839, 2842
PleI GAGTC 4 cut(s) 134, 288, 470, 1063
PpsI GAGTC 4 cut(s) 134, 288, 470, 1063
Ppu21I YACGTR 1 cut(s) 1225
PpuMI RGGWCCY 1 cut(s) 216
PscI ACATGT 1 cut(s) 2812
PshBI ATTAAT 1 cut(s) 2418
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 2908
PspEI GGTNACC 1 cut(s) 2648
PspFI CCCAGC 1 cut(s) 2843
PspGI CCWGG 1 cut(s) 2908
PspN4I GGNNCC 6 cut(s) 311, 807, 2190, 2452, 2728, 2793
PspPI GGNCC 4 cut(s) 216, 936, 2727, 2791
PspPPI RGGWCCY 1 cut(s) 216
PspXI VCTCGAGB 2 cut(s) 142, 2803
PstI CTGCAG 2 cut(s) 294, 2534
PstNI CAGNNNCTG 3 cut(s) 219, 1286, 2843
PsuI RGATCY 3 cut(s) 2299, 2458, 2629
PsyI GACNNNGTC 1 cut(s) 1601
PvuII CAGCTG 1 cut(s) 2843
RsaI GTAC 9 cut(s) 171, 311, 876, 1223, 1229, 1736, 1992, 2273, 2907
RsaNI GTAC 9 cut(s) 170, 310, 875, 1222, 1228, 1735, 1991, 2272, 2906
RseI CAYNNNNRTG 4 cut(s) 926, 1004, 1068, 2745
SacI GAGCTC 1 cut(s) 342
SacII CCGCGG 1 cut(s) 2167
SapI GCTCTTC 1 cut(s) 385
SaqAI TTAA 7 cut(s) 183, 1074, 1247, 1514, 2049, 2418, 2691
SatI GCNGC 8 cut(s) 423, 432, 986, 1936, 2533, 2542, 2838, 2841
Sau3AI GATC 4 cut(s) 1085, 2299, 2458, 2629
Sau96I GGNCC 4 cut(s) 216, 936, 2727, 2791
SchI GAGTC 4 cut(s) 134, 289, 471, 1064
ScrFI CCNGG 6 cut(s) 364, 832, 1159, 1762, 2795, 2910
SduI GDGCHC 6 cut(s) 342, 698, 1331, 1533, 2455, 2683
SfaNI GCATC 6 cut(s) 1285, 1563, 1747, 2766, 2808, 2848
SfcI CTRYAG 3 cut(s) 290, 2530, 2731
Sfr274I CTCGAG 2 cut(s) 142, 2803
Sfr303I CCGCGG 1 cut(s) 2167
SgrBI CCGCGG 1 cut(s) 2167
SinI GGWCC 2 cut(s) 216, 2791
SlaI CTCGAG 2 cut(s) 142, 2803
SmiMI CAYNNNNRTG 4 cut(s) 926, 1004, 1068, 2745
SmlI CTYRAG 3 cut(s) 142, 1337, 2803
SmoI CTYRAG 3 cut(s) 142, 1337, 2803
SphI GCATGC 2 cut(s) 1370, 1935
SseBI AGGCCT 2 cut(s) 148, 2289
SsiI CCGC 9 cut(s) 422, 849, 1343, 1936, 2164, 2166, 2343, 2541, 2582
SspMI CTAG 4 cut(s) 855, 1626, 2034, 2456
SstI GAGCTC 1 cut(s) 342
StuI AGGCCT 2 cut(s) 148, 2289
StyD4I CCNGG 6 cut(s) 362, 830, 1157, 1760, 2793, 2908
TaaI ACNGT 6 cut(s) 709, 1745, 2067, 2206, 2429, 2444
TaiI ACGT 4 cut(s) 398, 556, 1227, 1514
TaqI TCGA 6 cut(s) 89, 143, 791, 2550, 2607, 2804
TatI WGTACW 3 cut(s) 1227, 1734, 1990
TauI GCSGC 3 cut(s) 425, 1938, 2544
TfiI GAWTC 9 cut(s) 49, 664, 869, 1151, 2393, 2714, 2736, 2786, 2937
Tru1I TTAA 7 cut(s) 183, 1074, 1247, 1514, 2049, 2418, 2691
Tru9I TTAA 7 cut(s) 183, 1074, 1247, 1514, 2049, 2418, 2691
TscAI CASTG 5 cut(s) 712, 1153, 1541, 1639, 2688
TseFI GTSAC 4 cut(s) 997, 1603, 2200, 2808
TseI GCWGC 5 cut(s) 431, 985, 2532, 2837, 2840
Tsp45I GTSAC 4 cut(s) 997, 1603, 2200, 2808
TspDTI ATGAA 9 cut(s) 215, 446, 1052, 1143, 1658, 2014, 2099, 2145, 2489
TspGWI ACGGA 5 cut(s) 98, 642, 950, 1000, 2386
TspRI CASTG 5 cut(s) 712, 1153, 1541, 1639, 2688
Tth111I GACNNNGTC 1 cut(s) 1601
Van91I CCANNNNNTGG 1 cut(s) 1503
VneI GTGCAC 1 cut(s) 1529
VpaK11BI GGWCC 2 cut(s) 216, 2791
VspI ATTAAT 1 cut(s) 2418
XagI CCTNNNNNAGG 1 cut(s) 2657
XapI RAATTY 7 cut(s) 570, 688, 1030, 1243, 1492, 1717, 1918
XceI RCATGY 6 cut(s) 350, 1370, 1735, 1935, 2343, 2816
XhoI CTCGAG 2 cut(s) 142, 2803
XmiI GTMKAC 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 1388
XspI CTAG 4 cut(s) 855, 1626, 2034, 2456
Zsp2I ATGCAT 2 cut(s) 744, 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.