Rw2G014070

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012566
Physical Location & Seq
Reverse (-)
15536985 .. 15539954
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014070.1

Sequence Viewer

Length: 2937 bp
ATGGGATTGTTTTTCCGACTCCGGAACACTCTTTCTTCTCCTCCGGCGATAACCTCCTTTGTCAGACACAAACACCATTCAATCAATCCCAAAGCCTCACACTTCCTTGGTCTGCTCAATCTCTGCCGAACCGGCGAGGACTTGAAACCCCTCAAGTCCTTTCTCTTTGCTCATGGACTCATCAACCATGTCTCTTTGTTAGAACAATTTTTCAGGTCCTGTTTTCATCTGGGTGCTAAACATTTAGCTCTCTCTACATTCCAACAAATCAAAAGGCCGAGTCTTGGCTTGCAGAACCTGTTGATCAGGTGTCTTTGCAATCATGGCTTATACCAAGACCTTTTCTATGCGTATATGAAATCTCGGCTTTCGGGTTGCCCTTCTGATGATTTCACATTTCCGTTTGTGATCAAGGCCTGTGCGGCTTTGGGAGCTGTTAAGATTGGTAGAGAGGTTCATGGTGTTGTGTTCAGAACTGGGTTTGAGCAGAACCTTGTTATACAGACTAGTTTGGTTGACTTCTATGCAAGAACTGGGTGTGTGGAAACTGCACGCCAAGTGGTCGATAGAATTCCCCAACCGGACTTGGTTCCTTGGAATGCATTGATAGCTGGTTATTCGTTGAATGGATTCGATTGGGAAGCGTTAGAGGTTTTCAGGAAGATTGTTTTCAGTGATCTGAGGCCGAATTTGAGTACTCTGGCTAGCATTGTTCCTGTTTGCACTCGTTTGGGGTGCGTTCGTTCTGGTGAGTCTCTTCATTGCTTTGCTGTCAAGTCTGGATACTTTTCAAATGATTTTCTGGTTCCCGCTTTGATTTCAATGTACGCGGGGGATGGGGATATATGTGGTGCTAGAAAGTTGTTTGATTCTGTAGTGGAAAAGAATGTTGCTGTTTGGAATGCCATGATTTCTGCATATACACAAAGGCAAGAGCCTGTGTTAGCTTTTGAGTTGTTCCGGTGGATGCTGCTAGTTGACATAAGGCCTAATTTGGTCACTTTCGTGTCGATTATTCCCTCATTTGAGAATTTCATTAGCCTTTCTTTTGGCGAGTCTCTTCATGCTTGTGTAGTTAAACATGGATCAGAAAATCAGCTTCCGGTATTAACTGCCCTGGTATCAATGTATGCCAAACTTGGCAGTATTGATGAATCCAGGTATCTTTTCGACCAGATGCACAGTAAAAACCTTCTTCTGTGGAATTCAATGATTTCTGGGTACGTGTACAATGGGTTATGGGATTTAAGTCTGGGTTTGTTTCGTGAAATGCAAGTTTCAAGATTTGATGCGGATGCAGTCTCTGTTATTAGTATAATTTCTGCTTGCTCTAAACTTGAGGCTGATTTGTTGGGTAGGTCTGCCCATGCATTTAGTATAAGAAAGGGTTTCCACTCAAACATCAATCTGTCAAATGCATTGTTGGCATTTTACTCTGATTGTCATCTTCTCTCTTATTCTATTACTTTGTTTCACAAAATGCCTCTAAGAAATGGCATAACGTGGAATACATTAATTTCCAGTTGTGTGCACCATGGAGAAACGGACGGAGCAGTTGCTCTATACTACCAGATGCAGAAAGAGGGTTTCAAGTTGGACTTGGTCACCTTGACCAGTATACTCCCTAGTTGGAGTGACGAGGAAAACTTAGGGCAAGGAATGGCTTTTCATGGTTACGCTATAAAACATGGGTTTGCTTCAGACATTTCTCTGCTTAACTCACTTATTTGTATGTACTTTAACAGTGGAGACCTTGATGCTGGGAGGTTGGTTTTCGAAACCATGCCCAAAAGAACTGTGGTGTCTTGGAATGCTCTGATGACTGGCTTCAAGAATCATAATTTACAGAATGAGGTTATGGCCGTCTTTGGTCAAATGATAAAGGATGATCAGAGACCAAATTATGTAACTTTGCTAAATTTATTGCCTGCATGCTACTCCCAGTTACAGGGTAAGTCGATTCATGCTCTGGCAGTTAGAACAAGTATTCTCCATGAAACCCCTCTTCTTTCATCACTCATGTTTATGTATGCCAGATTTGACAACCATGATTCATGCATCTTACTGTTTAAAACGAGAAAAATGGAGGATATTTCTTTATGGAATGCCATTATGTCGGTGCAAATACAAGCAAAAAGTAGCGAGAATGCAGTGGGATTTTTCAATAGCTTGCTCCAGATGAAATTGGAACCAGACAATTTGACCCTTCTGAATTTGGTCTCTTCATGTGCTCAGCTGAATAGCCTCACTCTTGCTAATTCTGTAATGGGTTACATCATTCGCAAGGGCTTTGACAAAGATCTGGTTATTAGTAATGCACTAATAGATTTGCATTCAAGATGTGGAAACATATGCATTGCGAGAAAGCTATTTGATGGGTTGGTCAAGAGAGATGCTGTGTCTTGGAGTGTGATGATTAATGGGTATGGCTTGAATGGGAATGGCGAAGCTGCCCTAGATCTTTTCTTGCAGATGAAGCTATCAGGGATAGCACCTAATGGGGTCACTTATTTAAGTGTTTTATCAGCCTGCAGCCATTCTGGATTGGTTGAGCAAGGCCGCGCCGTATTCCAATCTATGGCAGAACATGGGATAACACGCCAAATGAAGCACTATGCTTGCATGGTGGACCTTCTAGGAAGAACGGGTAATTTGACTGAGGCTTATGACATTGTTCGGGAACTACCCTGTAAACCTTCAACAAGCCTACTTGAGTCTCTGCTGGGTGCTTGTAGAATTCATGGGAACGTTGAACTTGGACAGAAAATTGGTGGGTTGCTCTCTGAATTGGACCCTGAAAATTCAAGACCACATATAATGCTTCATAATATTTATGCCGCAGCTGGGAGATGGACTGATGCTGGTAGAGTAAGGTCTAAGATTGAAGAGAGAAGTTTGAGAAAAGTTCCTGGTTTCAGTCTTCTAATGGGATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 WEMBL

Protein Analysis

978

Amino Acids

108.92

Weight (kDa)

8.36

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 295 - 340 5e-08 PPR repeat family
PPR PF01535 401 - 427 6.4e-06 PPR repeat
PPR_2 PF13041 500 - 541 2.5e-08 PPR repeat family
PPR PF01535 500 - 530 5.4e-07 PPR repeat
PPR_2 PF13041 798 - 845 8e-11 PPR repeat family
PPR PF01535 800 - 830 4.3e-07 PPR repeat
E_motif PF20431 914 - 973 1.4e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2578
AccI GTMKAC 1 cut(s) 1618
AccII CGCG 2 cut(s) 830, 2562
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 6 cut(s) 422, 810, 830, 1292, 2560, 2838
AclI AACGTT 1 cut(s) 2748
AclWI GGATC 1 cut(s) 1093
AcoI YGGCCR 1 cut(s) 1860
AcsI RAATTY 8 cut(s) 570, 688, 1030, 1204, 1918, 2212, 2736, 2800
AcuI CTGAAG 1 cut(s) 1683
AfaI GTAC 5 cut(s) 697, 827, 1223, 1229, 1736
AfiI CCNNNNNNNGG 4 cut(s) 284, 1948, 2578, 2844
AflIII ACRYGT 1 cut(s) 1224
AhlI ACTAGT 1 cut(s) 506
AjnI CCWGG 3 cut(s) 1116, 1157, 2908
AleI CACNNNNGTG 1 cut(s) 1004
Alw21I GWGCWC 2 cut(s) 1533, 2233
Alw26I GTCTC 8 cut(s) 196, 759, 1062, 1306, 1743, 1888, 2224, 2721
Alw44I GTGCAC 1 cut(s) 1529
AlwI GGATC 1 cut(s) 1093
AlwNI CAGNNNCTG 3 cut(s) 219, 298, 1304
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 6 cut(s) 275, 414, 683, 986, 1860, 2557
ApaLI GTGCAC 1 cut(s) 1529
ApeKI GCWGC 4 cut(s) 970, 2450, 2532, 2840
ApoI RAATTY 8 cut(s) 570, 688, 1030, 1204, 1918, 2212, 2736, 2800
AseI ATTAAT 2 cut(s) 1514, 2418
Asp700I GAANNNNTTC 1 cut(s) 629
AspLEI GCGC 1 cut(s) 2564
AspS9I GGNCC 3 cut(s) 216, 2629, 2791
AsuHPI GGTGA 2 cut(s) 761, 1597
AsuII TTCGAA 1 cut(s) 1776
AsuNHI GCTAGC 1 cut(s) 704
AvaII GGWCC 3 cut(s) 216, 2629, 2791
BaeGI GKGCMC 1 cut(s) 1533
BbsI GAAGAC 1 cut(s) 2912
Bbv12I GWGCWC 2 cut(s) 1533, 2233
BbvI GCAGC 4 cut(s) 957, 2437, 2544, 2852
BccI CCATC 3 cut(s) 830, 2369, 2844
BceAI ACGGC 2 cut(s) 1847, 2549
BciT130I CCWGG 3 cut(s) 1118, 1159, 2910
BciVI GTATCC 1 cut(s) 776
BclI TGATCA 3 cut(s) 303, 408, 1888
BcoDI GTCTC 8 cut(s) 196, 759, 1062, 1306, 1743, 1888, 2224, 2721
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 7 cut(s) 507, 705, 855, 974, 1626, 2456, 2636
BfmI CTRYAG 2 cut(s) 873, 2530
BfuI GTATCC 1 cut(s) 776
BglI GCCNNNNNGGC 2 cut(s) 132, 422
BglII AGATCT 2 cut(s) 2299, 2458
BisI GCNGC 7 cut(s) 423, 971, 2451, 2533, 2560, 2838, 2841
BlpI GCTNAGC 1 cut(s) 2232
BlsI GCNGC 7 cut(s) 424, 972, 2452, 2534, 2561, 2839, 2842
BmcAI AGTACT 1 cut(s) 697
Bme1390I CCNGG 3 cut(s) 1118, 1159, 2910
Bme18I GGWCC 3 cut(s) 216, 2629, 2791
BmgT120I GGNCC 3 cut(s) 216, 2629, 2791
BmiI GGNNCC 4 cut(s) 591, 807, 2190, 2793
BmrFI CCNGG 3 cut(s) 1118, 1159, 2910
BmrI ACTGGG 3 cut(s) 486, 543, 1936
BmsI GCATC 9 cut(s) 957, 1167, 1279, 1285, 1563, 1747, 2067, 2383, 2848
BmtI GCTAGC 1 cut(s) 708
BmuI ACTGGG 3 cut(s) 486, 543, 1936
BpiI GAAGAC 1 cut(s) 2912
BpmI CTGGAG 1 cut(s) 2159
Bpu1102I GCTNAGC 1 cut(s) 2232
Bpu14I TTCGAA 1 cut(s) 1776
BpuEI CTTGAG 3 cut(s) 137, 1358, 2732
BsaAI YACGTR 1 cut(s) 1225
BsaBI GATNNNNATC 1 cut(s) 1443
BsaI GGTCTC 3 cut(s) 1743, 1888, 2224
BsaJI CCNNGG 4 cut(s) 106, 593, 1116, 1534
BsaWI WCCGGW 4 cut(s) 21, 580, 960, 1102
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 1948, 2578, 2844
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 6 cut(s) 481, 538, 1521, 1614, 1828, 1942
Bse3DI GCAATG 2 cut(s) 760, 2355
Bse8I GATNNNNATC 1 cut(s) 1443
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 3 cut(s) 1118, 1159, 2910
BseDI CCNNGG 4 cut(s) 106, 593, 1116, 1534
BseGI GGATG 4 cut(s) 841, 972, 1300, 1891
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 4 cut(s) 284, 1948, 2578, 2844
BseMI GCAATG 2 cut(s) 760, 2355
BseMII CTCAG 3 cut(s) 671, 2246, 2649
BseNI ACTGG 6 cut(s) 481, 538, 1521, 1614, 1828, 1942
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 1533
BseXI GCAGC 4 cut(s) 957, 2437, 2544, 2852
BseYI CCCAGC 3 cut(s) 1760, 2722, 2843
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 2 cut(s) 830, 2562
BshFI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
BsiHKAI GWGCWC 2 cut(s) 1533, 2233
BsiSI CCGG 6 cut(s) 22, 44, 132, 581, 961, 1103
BslI CCNNNNNNNGG 4 cut(s) 284, 1948, 2578, 2844
BsmAI GTCTC 8 cut(s) 196, 759, 1062, 1306, 1743, 1888, 2224, 2721
BsmI GAATGC 6 cut(s) 604, 907, 1816, 2110, 2152, 2332
BsnI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
Bso31I GGTCTC 3 cut(s) 1743, 1888, 2224
Bsp119I TTCGAA 1 cut(s) 1776
Bsp1286I GDGCHC 2 cut(s) 1533, 2233
Bsp13I TCCGGA 1 cut(s) 21
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
Bsp1720I GCTNAGC 1 cut(s) 2232
Bsp19I CCATGG 1 cut(s) 1534
BspACI CCGC 6 cut(s) 422, 810, 830, 1292, 2560, 2838
BspANI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
BspCNI CTCAG 3 cut(s) 672, 2245, 2650
BspEI TCCGGA 1 cut(s) 21
BspFNI CGCG 2 cut(s) 830, 2562
BspLI GGNNCC 4 cut(s) 591, 807, 2190, 2793
BspMAI CTGCAG 1 cut(s) 2534
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 1 cut(s) 1093
BspT104I TTCGAA 1 cut(s) 1776
BspTNI GGTCTC 3 cut(s) 1743, 1888, 2224
BsrDI GCAATG 2 cut(s) 760, 2355
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 6 cut(s) 481, 538, 1521, 1614, 1828, 1942
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 4 cut(s) 106, 593, 1116, 1534
BssMI GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
BssNAI GTATAC 1 cut(s) 1619
BssT1I CCWWGG 3 cut(s) 106, 593, 1534
Bst1107I GTATAC 1 cut(s) 1619
Bst2UI CCWGG 3 cut(s) 1118, 1159, 2910
Bst4CI ACNGT 4 cut(s) 1184, 1745, 1798, 2067
Bst6I CTCTTC 5 cut(s) 762, 1065, 2010, 2227, 2880
BstAUI TGTACA 1 cut(s) 1227
BstBAI YACGTR 1 cut(s) 1225
BstBI TTCGAA 1 cut(s) 1776
BstC8I GCNNGC 9 cut(s) 290, 553, 706, 1327, 1929, 1933, 2171, 2530, 2620
BstDEI CTNAG 6 cut(s) 680, 1487, 1648, 2232, 2658, 2877
BstDSI CCRYGG 1 cut(s) 1534
BstEII GGTNACC 1 cut(s) 1603
BstF5I GGATG 4 cut(s) 841, 972, 1300, 1891
BstFNI CGCG 2 cut(s) 830, 2562
BstHHI GCGC 1 cut(s) 2564
BstKTI GATC 7 cut(s) 306, 411, 679, 1088, 1891, 2302, 2461
BstMAI GTCTC 8 cut(s) 196, 759, 1062, 1306, 1743, 1888, 2224, 2721
BstMBI GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
BstMWI GCNNNNNNNGC 7 cut(s) 132, 324, 422, 431, 608, 1424, 2476
BstNI CCWGG 3 cut(s) 1118, 1159, 2910
BstNSI RCATGY 1 cut(s) 1935
BstPI GGTNACC 1 cut(s) 1603
BstSCI CCNGG 3 cut(s) 1116, 1157, 2908
BstSFI CTRYAG 2 cut(s) 873, 2530
BstSLI GKGCMC 1 cut(s) 1533
BstUI CGCG 2 cut(s) 830, 2562
BstV1I GCAGC 4 cut(s) 957, 2437, 2544, 2852
BstV2I GAAGAC 1 cut(s) 2912
BstX2I RGATCY 2 cut(s) 2299, 2458
BstYI RGATCY 2 cut(s) 2299, 2458
BstZ17I GTATAC 1 cut(s) 1619
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
BtgI CCRYGG 1 cut(s) 1534
BtsCI GGATG 4 cut(s) 841, 972, 1300, 1891
BtsI GCAGTG 1 cut(s) 2157
BtsIMutI CAGTG 3 cut(s) 679, 1750, 2157
Cac8I GCNNGC 9 cut(s) 290, 553, 706, 1327, 1929, 1933, 2171, 2530, 2620
CaiI CAGNNNCTG 3 cut(s) 219, 298, 1304
CfoI GCGC 1 cut(s) 2564
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 216, 2629, 2791
Csp6I GTAC 5 cut(s) 696, 826, 1222, 1228, 1735
CviQI GTAC 5 cut(s) 696, 826, 1222, 1228, 1735
DdeI CTNAG 6 cut(s) 680, 1487, 1648, 2232, 2658, 2877
DpnI GATC 7 cut(s) 305, 410, 678, 1087, 1890, 2301, 2460
DpnII GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
DraI TTTAAA 1 cut(s) 2071
EaeI YGGCCR 1 cut(s) 1860
Eam1104I CTCTTC 5 cut(s) 762, 1065, 2010, 2227, 2880
EarI CTCTTC 5 cut(s) 762, 1065, 2010, 2227, 2880
Eco130I CCWWGG 3 cut(s) 106, 593, 1534
Eco147I AGGCCT 2 cut(s) 416, 988
Eco31I GGTCTC 3 cut(s) 1743, 1888, 2224
Eco47I GGWCC 3 cut(s) 216, 2629, 2791
Eco57I CTGAAG 1 cut(s) 1683
Eco91I GGTNACC 1 cut(s) 1603
EcoO109I RGGNCCY 1 cut(s) 216
EcoO65I GGTNACC 1 cut(s) 1603
EcoRI GAATTC 3 cut(s) 570, 1204, 2736
EcoRII CCWGG 3 cut(s) 1116, 1157, 2908
EcoT14I CCWWGG 3 cut(s) 106, 593, 1534
EcoT22I ATGCAT 5 cut(s) 604, 1372, 1420, 2060, 2357
ErhI CCWWGG 3 cut(s) 106, 593, 1534
FalI AAGNNNNNCTT 2 cut(s) 1583, 1615
FauI CCCGC 2 cut(s) 817, 823
FauNDI CATATG 1 cut(s) 2351
FbaI TGATCA 3 cut(s) 303, 408, 1888
FblI GTMKAC 1 cut(s) 1618
Fnu4HI GCNGC 7 cut(s) 423, 971, 2451, 2533, 2560, 2838, 2841
FokI GGATG 4 cut(s) 848, 979, 1307, 1898
Fsp4HI GCNGC 7 cut(s) 423, 971, 2451, 2533, 2560, 2838, 2841
FspBI CTAG 7 cut(s) 507, 705, 855, 974, 1626, 2456, 2636
GlaI GCGC 1 cut(s) 2563
GluI GCNGC 7 cut(s) 423, 971, 2451, 2533, 2560, 2838, 2841
GsaI CCCAGC 3 cut(s) 1764, 2726, 2847
GsuI CTGGAG 1 cut(s) 2159
HaeIII GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
HapII CCGG 6 cut(s) 22, 44, 132, 581, 961, 1103
HhaI GCGC 1 cut(s) 2564
Hin6I GCGC 1 cut(s) 2562
HinP1I GCGC 1 cut(s) 2562
HincII GTYRAC 2 cut(s) 517, 979
HindII GTYRAC 2 cut(s) 517, 979
HpaII CCGG 6 cut(s) 22, 44, 132, 581, 961, 1103
HphI GGTGA 2 cut(s) 761, 1597
Hpy166II GTNNAC 7 cut(s) 517, 979, 1228, 1531, 1619, 2629, 2693
Hpy8I GTNNAC 7 cut(s) 517, 979, 1228, 1531, 1619, 2629, 2693
HpyAV CCTTC 5 cut(s) 390, 1202, 2216, 2642, 2706
HpyCH4III ACNGT 4 cut(s) 1184, 1745, 1798, 2067
HpyCH4IV ACGT 3 cut(s) 1224, 1502, 2748
HpyF10VI GCNNNNNNNGC 7 cut(s) 132, 324, 422, 431, 608, 1424, 2476
HpyF3I CTNAG 6 cut(s) 680, 1487, 1648, 2232, 2658, 2877
HpySE526I ACGT 3 cut(s) 1224, 1502, 2748
HspAI GCGC 1 cut(s) 2562
Kpn2I TCCGGA 1 cut(s) 21
Ksp22I TGATCA 3 cut(s) 303, 408, 1888
Kzo9I GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
LmnI GCTCC 3 cut(s) 431, 1550, 2178
Lsp1109I GCAGC 4 cut(s) 957, 2437, 2544, 2852
LweI GCATC 9 cut(s) 957, 1167, 1279, 1285, 1563, 1747, 2067, 2383, 2848
MaeI CTAG 7 cut(s) 507, 705, 855, 974, 1626, 2456, 2636
MaeII ACGT 3 cut(s) 1224, 1502, 2748
MaeIII GTNAC 8 cut(s) 997, 1603, 1634, 1673, 1906, 1944, 2270, 2503
MalI GATC 7 cut(s) 305, 410, 678, 1087, 1890, 2301, 2460
MboI GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
MflI RGATCY 2 cut(s) 2299, 2458
MhlI GDGCHC 2 cut(s) 1533, 2233
MlyI GAGTC 6 cut(s) 12, 171, 289, 761, 1064, 2723
MmeI TCCRAC 4 cut(s) 40, 286, 1575, 1610
Mph1103I ATGCAT 5 cut(s) 604, 1372, 1420, 2060, 2357
MroI TCCGGA 1 cut(s) 21
MroXI GAANNNNTTC 1 cut(s) 629
MslI CAYNNNNRTG 4 cut(s) 231, 1004, 1068, 2024
MspA1I CMGCKG 2 cut(s) 2236, 2843
MspI CCGG 6 cut(s) 22, 44, 132, 581, 961, 1103
MspR9I CCNGG 3 cut(s) 1118, 1159, 2910
Mva1269I GAATGC 6 cut(s) 604, 907, 1816, 2110, 2152, 2332
MvaI CCWGG 3 cut(s) 1118, 1159, 2910
MvnI CGCG 2 cut(s) 830, 2562
MwoI GCNNNNNNNGC 7 cut(s) 132, 324, 422, 431, 608, 1424, 2476
NcoI CCATGG 1 cut(s) 1534
NdeI CATATG 1 cut(s) 2351
NdeII GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
NheI GCTAGC 1 cut(s) 704
NlaIV GGNNCC 4 cut(s) 591, 807, 2190, 2793
NmeAIII GCCGAG 2 cut(s) 303, 343
NmuCI GTSAC 4 cut(s) 997, 1603, 1634, 2503
NsiI ATGCAT 5 cut(s) 604, 1372, 1420, 2060, 2357
NspI RCATGY 1 cut(s) 1935
NspV TTCGAA 1 cut(s) 1776
OliI CACNNNNGTG 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 1935
PceI AGGCCT 2 cut(s) 416, 988
PctI GAATGC 6 cut(s) 604, 907, 1816, 2110, 2152, 2332
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 6 cut(s) 630, 869, 1154, 1834, 1960, 2051
PflFI GACNNNGTC 1 cut(s) 1601
PflMI CCANNNNNTGG 1 cut(s) 2578
PkrI GCNGC 7 cut(s) 424, 972, 2452, 2534, 2561, 2839, 2842
PleI GAGTC 6 cut(s) 12, 171, 288, 760, 1063, 2722
PpsI GAGTC 6 cut(s) 12, 171, 288, 760, 1063, 2722
Ppu21I YACGTR 1 cut(s) 1225
PpuMI RGGWCCY 1 cut(s) 216
PshBI ATTAAT 2 cut(s) 1514, 2418
Psp1406I AACGTT 1 cut(s) 2748
Psp5II RGGWCCY 1 cut(s) 216
Psp6I CCWGG 3 cut(s) 1116, 1157, 2908
PspEI GGTNACC 1 cut(s) 1603
PspFI CCCAGC 3 cut(s) 1760, 2722, 2843
PspGI CCWGG 3 cut(s) 1116, 1157, 2908
PspN4I GGNNCC 4 cut(s) 591, 807, 2190, 2793
PspPI GGNCC 3 cut(s) 216, 2629, 2791
PspPPI RGGWCCY 1 cut(s) 216
PstI CTGCAG 1 cut(s) 2534
PstNI CAGNNNCTG 3 cut(s) 219, 298, 1304
PsuI RGATCY 2 cut(s) 2299, 2458
PsyI GACNNNGTC 1 cut(s) 1601
PvuII CAGCTG 2 cut(s) 2236, 2843
RsaI GTAC 5 cut(s) 697, 827, 1223, 1229, 1736
RsaNI GTAC 5 cut(s) 696, 826, 1222, 1228, 1735
RseI CAYNNNNRTG 4 cut(s) 231, 1004, 1068, 2024
SatI GCNGC 7 cut(s) 423, 971, 2451, 2533, 2560, 2838, 2841
Sau3AI GATC 7 cut(s) 303, 408, 676, 1085, 1888, 2299, 2458
Sau96I GGNCC 3 cut(s) 216, 2629, 2791
ScaI AGTACT 1 cut(s) 697
SchI GAGTC 6 cut(s) 12, 171, 289, 761, 1064, 2723
ScrFI CCNGG 3 cut(s) 1118, 1159, 2910
SduI GDGCHC 2 cut(s) 1533, 2233
SfaNI GCATC 9 cut(s) 957, 1167, 1279, 1285, 1563, 1747, 2067, 2383, 2848
SfcI CTRYAG 2 cut(s) 873, 2530
SfuI TTCGAA 1 cut(s) 1776
SinI GGWCC 3 cut(s) 216, 2629, 2791
SmiMI CAYNNNNRTG 4 cut(s) 231, 1004, 1068, 2024
SmlI CTYRAG 3 cut(s) 152, 1337, 2711
SmoI CTYRAG 3 cut(s) 152, 1337, 2711
SpeI ACTAGT 1 cut(s) 506
SphI GCATGC 1 cut(s) 1935
SseBI AGGCCT 2 cut(s) 416, 988
SsiI CCGC 6 cut(s) 422, 810, 830, 1292, 2560, 2838
SspI AATATT 1 cut(s) 2830
SspMI CTAG 7 cut(s) 507, 705, 855, 974, 1626, 2456, 2636
StuI AGGCCT 2 cut(s) 416, 988
StyD4I CCNGG 3 cut(s) 1116, 1157, 2908
StyI CCWWGG 3 cut(s) 106, 593, 1534
TaaI ACNGT 4 cut(s) 1184, 1745, 1798, 2067
TaiI ACGT 3 cut(s) 1227, 1505, 2751
TaqI TCGA 6 cut(s) 564, 633, 1010, 1170, 1776, 1958
TatI WGTACW 3 cut(s) 695, 1227, 1734
TauI GCSGC 3 cut(s) 425, 2562, 2840
TfiI GAWTC 6 cut(s) 630, 869, 1154, 1834, 1960, 2051
TscAI CASTG 3 cut(s) 679, 1750, 2157
TseFI GTSAC 4 cut(s) 997, 1603, 1634, 2503
TseI GCWGC 4 cut(s) 970, 2450, 2532, 2840
Tsp45I GTSAC 4 cut(s) 997, 1603, 1634, 2503
TspGWI ACGGA 3 cut(s) 390, 1559, 1563
TspRI CASTG 3 cut(s) 679, 1750, 2157
Tth111I GACNNNGTC 1 cut(s) 1601
Van91I CCANNNNNTGG 1 cut(s) 2578
VneI GTGCAC 1 cut(s) 1529
VpaK11BI GGWCC 3 cut(s) 216, 2629, 2791
VspI ATTAAT 2 cut(s) 1514, 2418
XapI RAATTY 8 cut(s) 570, 688, 1030, 1204, 1918, 2212, 2736, 2800
XceI RCATGY 1 cut(s) 1935
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XmiI GTMKAC 1 cut(s) 1618
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 7 cut(s) 507, 705, 855, 974, 1626, 2456, 2636
ZrmI AGTACT 1 cut(s) 697
Zsp2I ATGCAT 5 cut(s) 604, 1372, 1420, 2060, 2357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.