Rh2DG185700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012566
Physical Location & Seq
Reverse (-)
16585048 .. 16588191
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG185700.1

Sequence Viewer

Length: 2976 bp
ATGCTCTGTTTCTCTAAACCCAAGATCTCTAATGGGATAATGGGATTGTTTTTCCGACTCCGGAACACTCTTTCTTCTCCTCCGGCGATAACCTCCTTTGTCAGACACAAACACCATTCAATCAATCCCAAAGCCTCACACTTCCTTGGTCTGCTCAATCTCTGCCGAACCGGCGAGGACTTGAAACCCCTCAAGTCCTTTCTCTTTGCTCATGGACTCATCAACCATGTCTCTTTGTTGGAACAATTTTTCAGGTCCTGTTTTCATCTGGGTGCTTCACATTTAGCTCTCTCTACATTCCAACAAATCAAAAGGCCGAGTCTTGGCTTGCAGAACCTGTTGATCAGGTGTCTTTGCAATCATGGCTTATACCAAGACCTTTTCTATGTGTATATGAAATCTCGGCTTTCGGGTTGCCCTTCTGATGATTTCACATTTCCGTTTGTGATCAAGGCCTGTGCGGCTTTGGGAGCTGTTAAGATTGGTAGAGAGGTTCATGGTGTTGTGTTCAGAACTGGGTTTGAGCAGAACCTTGTTATACAGACTAGTTTGGTTGACTTCTATGCAAGAACTGGGTGTGTGGAAACTGCACGCCAAGTGATCGATAGAATTCCCCAACCGGACTTGGTTCCTTGGAATGCATTGATAGCTGGTTATTCGTTGAATGGATTCGACTGGGAAGCGTTAGAGGTTTTCAGGAAGATTGTTTTCAGTGATCTGAGGCCGAATTTGAGTACTCTGGCTAGCATTGTTCCTGTTTGCACTCGTTTGGGGTGTGTTCGTGCTGGTGAGTCTCTTCATTGCTTTGCTGTCAAGTCTGGATACTTTTCAAATGATTTTCTGCTTCCTGCTTTGATTTCAATGTACGCCGGGGATGGGGATATATGTGGTGCTAGAAAGTTGTTTGATTCTGTAGTGGAAAAGAATGTTGCTGTTTGGAATGCCATGATTTCTGCATATACACAAAGGCAAGAGCCTGTGTTAGCTTTTGAGTTGTTCCGGTGGATGCTGCTAGTTGACATAAGGCCTAATTTGGTCACTTTCGTGTCGATTATTCCCTCATTTGAGAATTTCATTAGCCTTTCTTTTGGCGAGTCTCTTCATGCTTGTGTAGTTAAACATGGATCAGAAAATCAGCTTCCGGTATTAACTGCCCTGGTATCAATGTATGCCAAACTTGGCAGTATTGATGAATCCAGGTATCTTTTCGACCAGATGCACAGTAAAAACCTTCTTCTGTGGAATTCAATGATTTCTGGGTACGTGTACAATGGGTTATGGGATTTAAGTCTGGGTTTGTTTCGTGAAATGCAAGTTTCAAGATTTGATGCGGATGCAGTCTCTGTTATTAGTATAATTTCTGCTTGCTCTAAACTTGAGGCTGATTTGTTGGGTAGGTCTGCCCATGCATTTAGTATAAGAAAGGGTTTCCACTCAAACATCAATCTGTCAAATGCATTGTTGGCATTTTACTCTGATTGTCATCTTCTCTCTTATTCTATTACTTTGTTTCACAAAATGCCTCTAAGAAATGGCATAACGTGGAATACATTAATTTCCAGTTGTGTGCACCATGGAGAAACGGACGGAGCAGTTGCTCTATACTACCAGATGCAGAAAGAGGGTTTCAAGTTGGACTTGGTCACCTTGATCAGTATACTCCCTAGTTGGAGTGACGAGGAAAACTTAGGGCAAGGAATGGCTTTTCATGGTTACGCTATAAAACATGGGTTTGCTTCTGACATTTCTCTGCTTAACTCACTTATTTGTATGTACTTTAACAGTGGAGACCTTGATGCTGGGAGGTTGGTTTTCGAAACCATGCCCAAAAGAACTGTGGTGTCTTGGAATGCTCTGATGACTGGCTTCAAGAATCATAATTTACAGAATGAGGTTATGGCCGTCTTTGGTCAAATGATAAAGGATGATCAGAGACCAAATTATGTAACTTTGCTAAATTTATTGCCTGCATGCTACTCCCAGTTACAGGGTAAGTCTATTCATGCTCTGGCAGTTAGAACAAGTATTCTCCATGAAACCCCTCTTCTTTCATCACTCATGTTTATGTATGCCAGATTTGACAACCATGATTCATGCATCTTACTGTTTAAAACGAGAAAAATGGAGGATATTTCTTTGTGGAATGCCATTATGTCGGTGCAAATACAAGCAAAAAGTAGCGAGAATGCAGTGGGATTTTTCAATAGCTTGCTCCAGATGAAATTGGAACCAGACAATTTGACCCTTCTGAATTTGGTCTCTTCATGTGCTCAGCTGAATAGCCTCACTCTTGCTAATTCTGTAATGGGTTACATCATTCGCAAGGGCTTTGACAAAGATCTGGTTATTAGTAATGCACTAATAGATTTGCATTCAAGATGTGGAAACATATGCATTGCGAGAAAGCTGTTTGATGGGTTGGTCAAGAGAGATGCTGTGTCTTGGAGTGTGATGATTAATGGGTATGGCTTGAATGGGAATGGCGAAGCTGCCCTAGATCTTTTCTTGCAGATGAAGCTATCAGGGATAGCACCTAATGGGGTTACTTATTTAAGTGTTTTATCAGCCTGCAGCCATTCTGGATTGGTTGAGCAAGGCCGCGCCGTATTCAAATCTATGGCAGAACATGGGATAACACGCCAAATGAAGCACTATGCTTGCATGGTGGACCTTCTAGGAAGAACGGGTAATTTGACTGAGGCTTATGACATTGTTCGGGAACTACCCTGTAAACCTTCAACAAGCCTACTTGAGTCTCTGCTGGGTGCTTGTAGAATTCATGGGAACGTTGAACTTGGACAGAAAATTGGTGGGTTGCTCTCTGAATTGGACCCTGAAAATTCAAGACCACATGTAATGCTTCATAATATTTATGCCGCAGCTGGGAGATGGACTGATGCTGGTAGAGTAAGGTCTAGGATTGAAGAGAGAAGTTTGAGAAAAGTTCCTGGTTTCAGTCTTCTAATGGGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 WEMBL

Protein Analysis

991

Amino Acids

110.34

Weight (kDa)

8.49

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 308 - 353 5.1e-08 PPR repeat family
PPR PF01535 414 - 440 6.5e-06 PPR repeat
PPR_2 PF13041 513 - 550 2.4e-07 PPR repeat family
PPR PF01535 513 - 543 5.5e-07 PPR repeat
PPR_2 PF13041 811 - 858 8.1e-11 PPR repeat family
PPR PF01535 813 - 843 4.4e-07 PPR repeat
E_motif PF20431 927 - 986 3.6e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1657
AccII CGCG 1 cut(s) 2601
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 4 cut(s) 461, 1331, 2599, 2877
AclI AACGTT 1 cut(s) 2787
AclWI GGATC 1 cut(s) 1132
AcoI YGGCCR 1 cut(s) 1899
AcsI RAATTY 8 cut(s) 609, 727, 1069, 1243, 1957, 2251, 2775, 2839
AfaI GTAC 5 cut(s) 736, 866, 1262, 1268, 1775
AfiI CCNNNNNNNGG 4 cut(s) 323, 876, 1987, 2883
AflIII ACRYGT 2 cut(s) 1263, 2851
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 3 cut(s) 1155, 1196, 2947
AleI CACNNNNGTG 1 cut(s) 1043
Alw21I GWGCWC 2 cut(s) 1572, 2272
Alw26I GTCTC 8 cut(s) 235, 798, 1101, 1345, 1782, 1927, 2263, 2760
Alw44I GTGCAC 1 cut(s) 1568
AlwI GGATC 1 cut(s) 1132
AlwNI CAGNNNCTG 3 cut(s) 258, 337, 1343
Aor13HI TCCGGA 1 cut(s) 60
AoxI GGCC 6 cut(s) 314, 453, 722, 1025, 1899, 2596
ApaLI GTGCAC 1 cut(s) 1568
ApeKI GCWGC 4 cut(s) 1009, 2489, 2571, 2879
ApoI RAATTY 8 cut(s) 609, 727, 1069, 1243, 1957, 2251, 2775, 2839
AseI ATTAAT 2 cut(s) 1553, 2457
Asp700I GAANNNNTTC 1 cut(s) 668
AspLEI GCGC 1 cut(s) 2603
AspS9I GGNCC 3 cut(s) 255, 2668, 2830
AsuC2I CCSGG 1 cut(s) 871
AsuHPI GGTGA 2 cut(s) 800, 1636
AsuII TTCGAA 1 cut(s) 1815
AsuNHI GCTAGC 1 cut(s) 743
AvaII GGWCC 3 cut(s) 255, 2668, 2830
BaeGI GKGCMC 1 cut(s) 1572
BbsI GAAGAC 1 cut(s) 2951
Bbv12I GWGCWC 2 cut(s) 1572, 2272
BbvI GCAGC 4 cut(s) 996, 2476, 2583, 2891
BccI CCATC 3 cut(s) 869, 2408, 2883
BceAI ACGGC 2 cut(s) 1886, 2588
BciT130I CCWGG 3 cut(s) 1157, 1198, 2949
BciVI GTATCC 1 cut(s) 815
BclI TGATCA 4 cut(s) 342, 447, 1650, 1927
BcnI CCSGG 1 cut(s) 871
BcoDI GTCTC 8 cut(s) 235, 798, 1101, 1345, 1782, 1927, 2263, 2760
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 8 cut(s) 546, 744, 894, 1013, 1665, 2495, 2675, 2916
BfmI CTRYAG 2 cut(s) 912, 2569
BfuI GTATCC 1 cut(s) 815
BglI GCCNNNNNGGC 2 cut(s) 171, 461
BglII AGATCT 3 cut(s) 24, 2338, 2497
BisI GCNGC 7 cut(s) 462, 1010, 2490, 2572, 2599, 2877, 2880
BlpI GCTNAGC 1 cut(s) 2271
BlsI GCNGC 7 cut(s) 463, 1011, 2491, 2573, 2600, 2878, 2881
BmcAI AGTACT 1 cut(s) 736
Bme1390I CCNGG 4 cut(s) 871, 1157, 1198, 2949
Bme18I GGWCC 3 cut(s) 255, 2668, 2830
BmgT120I GGNCC 3 cut(s) 255, 2668, 2830
BmiI GGNNCC 3 cut(s) 630, 2229, 2832
BmrFI CCNGG 4 cut(s) 871, 1157, 1198, 2949
BmrI ACTGGG 4 cut(s) 525, 582, 685, 1975
BmsI GCATC 9 cut(s) 996, 1206, 1318, 1324, 1602, 1786, 2106, 2422, 2887
BmtI GCTAGC 1 cut(s) 747
BmuI ACTGGG 4 cut(s) 525, 582, 685, 1975
BpiI GAAGAC 1 cut(s) 2951
BpmI CTGGAG 1 cut(s) 2198
Bpu1102I GCTNAGC 1 cut(s) 2271
Bpu14I TTCGAA 1 cut(s) 1815
BpuEI CTTGAG 3 cut(s) 176, 1397, 2771
BpuMI CCSGG 1 cut(s) 871
Bsa29I ATCGAT 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 1264
BsaBI GATNNNNATC 1 cut(s) 1482
BsaI GGTCTC 3 cut(s) 1782, 1927, 2263
BsaJI CCNNGG 5 cut(s) 145, 632, 870, 1155, 1573
BsaWI WCCGGW 4 cut(s) 60, 619, 999, 1141
Bsc4I CCNNNNNNNGG 4 cut(s) 323, 876, 1987, 2883
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 6 cut(s) 520, 577, 680, 1560, 1867, 1981
Bse3DI GCAATG 2 cut(s) 799, 2394
Bse8I GATNNNNATC 1 cut(s) 1482
BseAI TCCGGA 1 cut(s) 60
BseBI CCWGG 3 cut(s) 1157, 1198, 2949
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 5 cut(s) 145, 632, 870, 1155, 1573
BseGI GGATG 4 cut(s) 880, 1011, 1339, 1930
BseJI GATNNNNATC 1 cut(s) 1482
BseLI CCNNNNNNNGG 4 cut(s) 323, 876, 1987, 2883
BseMI GCAATG 2 cut(s) 799, 2394
BseMII CTCAG 3 cut(s) 710, 2285, 2688
BseNI ACTGG 6 cut(s) 520, 577, 680, 1560, 1867, 1981
BseRI GAGGAG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 1572
BseXI GCAGC 4 cut(s) 996, 2476, 2583, 2891
BseYI CCCAGC 3 cut(s) 1799, 2761, 2882
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 1 cut(s) 2601
BshFI GGCC 6 cut(s) 316, 455, 724, 1027, 1901, 2598
BshVI ATCGAT 1 cut(s) 603
BsiHKAI GWGCWC 2 cut(s) 1572, 2272
BsiSI CCGG 7 cut(s) 61, 83, 171, 620, 870, 1000, 1142
BslI CCNNNNNNNGG 4 cut(s) 323, 876, 1987, 2883
BsmAI GTCTC 8 cut(s) 235, 798, 1101, 1345, 1782, 1927, 2263, 2760
BsmI GAATGC 6 cut(s) 643, 946, 1855, 2149, 2191, 2371
BsnI GGCC 6 cut(s) 316, 455, 724, 1027, 1901, 2598
Bso31I GGTCTC 3 cut(s) 1782, 1927, 2263
Bsp119I TTCGAA 1 cut(s) 1815
Bsp1286I GDGCHC 2 cut(s) 1572, 2272
Bsp13I TCCGGA 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 1266
Bsp1720I GCTNAGC 1 cut(s) 2271
Bsp19I CCATGG 1 cut(s) 1573
BspACI CCGC 4 cut(s) 461, 1331, 2599, 2877
BspANI GGCC 6 cut(s) 316, 455, 724, 1027, 1901, 2598
BspCNI CTCAG 3 cut(s) 711, 2284, 2689
BspDI ATCGAT 1 cut(s) 603
BspEI TCCGGA 1 cut(s) 60
BspFNI CGCG 1 cut(s) 2601
BspLI GGNNCC 3 cut(s) 630, 2229, 2832
BspMAI CTGCAG 1 cut(s) 2573
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 1 cut(s) 1132
BspT104I TTCGAA 1 cut(s) 1815
BspTNI GGTCTC 3 cut(s) 1782, 1927, 2263
BsrDI GCAATG 2 cut(s) 799, 2394
BsrFI RCCGGY 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 1266
BsrI ACTGG 6 cut(s) 520, 577, 680, 1560, 1867, 1981
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 5 cut(s) 145, 632, 870, 1155, 1573
BssNAI GTATAC 1 cut(s) 1658
BssT1I CCWWGG 3 cut(s) 145, 632, 1573
Bst1107I GTATAC 1 cut(s) 1658
Bst2UI CCWGG 3 cut(s) 1157, 1198, 2949
Bst4CI ACNGT 4 cut(s) 1223, 1784, 1837, 2106
Bst6I CTCTTC 5 cut(s) 801, 1104, 2049, 2266, 2919
BstAUI TGTACA 1 cut(s) 1266
BstBAI YACGTR 1 cut(s) 1264
BstBI TTCGAA 1 cut(s) 1815
BstC8I GCNNGC 9 cut(s) 329, 592, 745, 1366, 1968, 1972, 2210, 2569, 2659
BstDEI CTNAG 5 cut(s) 719, 1526, 1687, 2271, 2697
BstDSI CCRYGG 1 cut(s) 1573
BstEII GGTNACC 1 cut(s) 1642
BstF5I GGATG 4 cut(s) 880, 1011, 1339, 1930
BstFNI CGCG 1 cut(s) 2601
BstHHI GCGC 1 cut(s) 2603
BstMAI GTCTC 8 cut(s) 235, 798, 1101, 1345, 1782, 1927, 2263, 2760
BstMWI GCNNNNNNNGC 7 cut(s) 171, 363, 461, 470, 647, 1463, 2515
BstNI CCWGG 3 cut(s) 1157, 1198, 2949
BstNSI RCATGY 2 cut(s) 1974, 2855
BstPI GGTNACC 1 cut(s) 1642
BstSCI CCNGG 4 cut(s) 869, 1155, 1196, 2947
BstSFI CTRYAG 2 cut(s) 912, 2569
BstSLI GKGCMC 1 cut(s) 1572
BstUI CGCG 1 cut(s) 2601
BstV1I GCAGC 4 cut(s) 996, 2476, 2583, 2891
BstV2I GAAGAC 1 cut(s) 2951
BstX2I RGATCY 3 cut(s) 24, 2338, 2497
BstYI RGATCY 3 cut(s) 24, 2338, 2497
BstZ17I GTATAC 1 cut(s) 1658
Bsu15I ATCGAT 1 cut(s) 603
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 6 cut(s) 316, 455, 724, 1027, 1901, 2598
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 1573
BtsCI GGATG 4 cut(s) 880, 1011, 1339, 1930
BtsI GCAGTG 1 cut(s) 2196
BtsIMutI CAGTG 3 cut(s) 718, 1789, 2196
Cac8I GCNNGC 9 cut(s) 329, 592, 745, 1366, 1968, 1972, 2210, 2569, 2659
CaiI CAGNNNCTG 3 cut(s) 258, 337, 1343
CfoI GCGC 1 cut(s) 2603
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 3 cut(s) 255, 2668, 2830
ClaI ATCGAT 1 cut(s) 603
Csp6I GTAC 5 cut(s) 735, 865, 1261, 1267, 1774
CviQI GTAC 5 cut(s) 735, 865, 1261, 1267, 1774
DdeI CTNAG 5 cut(s) 719, 1526, 1687, 2271, 2697
DraI TTTAAA 1 cut(s) 2110
EaeI YGGCCR 1 cut(s) 1899
Eam1104I CTCTTC 5 cut(s) 801, 1104, 2049, 2266, 2919
EarI CTCTTC 5 cut(s) 801, 1104, 2049, 2266, 2919
Eco130I CCWWGG 3 cut(s) 145, 632, 1573
Eco147I AGGCCT 2 cut(s) 455, 1027
Eco31I GGTCTC 3 cut(s) 1782, 1927, 2263
Eco47I GGWCC 3 cut(s) 255, 2668, 2830
Eco91I GGTNACC 1 cut(s) 1642
EcoO109I RGGNCCY 1 cut(s) 255
EcoO65I GGTNACC 1 cut(s) 1642
EcoRI GAATTC 3 cut(s) 609, 1243, 2775
EcoRII CCWGG 3 cut(s) 1155, 1196, 2947
EcoT14I CCWWGG 3 cut(s) 145, 632, 1573
EcoT22I ATGCAT 5 cut(s) 643, 1411, 1459, 2099, 2396
ErhI CCWWGG 3 cut(s) 145, 632, 1573
FalI AAGNNNNNCTT 2 cut(s) 1622, 1654
FauNDI CATATG 1 cut(s) 2390
FbaI TGATCA 4 cut(s) 342, 447, 1650, 1927
FblI GTMKAC 1 cut(s) 1657
Fnu4HI GCNGC 7 cut(s) 462, 1010, 2490, 2572, 2599, 2877, 2880
FokI GGATG 4 cut(s) 887, 1018, 1346, 1937
Fsp4HI GCNGC 7 cut(s) 462, 1010, 2490, 2572, 2599, 2877, 2880
FspBI CTAG 8 cut(s) 546, 744, 894, 1013, 1665, 2495, 2675, 2916
GlaI GCGC 1 cut(s) 2602
GluI GCNGC 7 cut(s) 462, 1010, 2490, 2572, 2599, 2877, 2880
GsaI CCCAGC 3 cut(s) 1803, 2765, 2886
GsuI CTGGAG 1 cut(s) 2198
HaeIII GGCC 6 cut(s) 316, 455, 724, 1027, 1901, 2598
HapII CCGG 7 cut(s) 61, 83, 171, 620, 870, 1000, 1142
HhaI GCGC 1 cut(s) 2603
Hin6I GCGC 1 cut(s) 2601
HinP1I GCGC 1 cut(s) 2601
HincII GTYRAC 2 cut(s) 556, 1018
HindII GTYRAC 2 cut(s) 556, 1018
HpaII CCGG 7 cut(s) 61, 83, 171, 620, 870, 1000, 1142
HphI GGTGA 2 cut(s) 800, 1636
Hpy166II GTNNAC 7 cut(s) 556, 1018, 1267, 1570, 1658, 2668, 2732
Hpy8I GTNNAC 7 cut(s) 556, 1018, 1267, 1570, 1658, 2668, 2732
HpyAV CCTTC 5 cut(s) 429, 1241, 2255, 2681, 2745
HpyCH4III ACNGT 4 cut(s) 1223, 1784, 1837, 2106
HpyCH4IV ACGT 3 cut(s) 1263, 1541, 2787
HpyF10VI GCNNNNNNNGC 7 cut(s) 171, 363, 461, 470, 647, 1463, 2515
HpyF3I CTNAG 5 cut(s) 719, 1526, 1687, 2271, 2697
HpySE526I ACGT 3 cut(s) 1263, 1541, 2787
HspAI GCGC 1 cut(s) 2601
Kpn2I TCCGGA 1 cut(s) 60
Ksp22I TGATCA 4 cut(s) 342, 447, 1650, 1927
LmnI GCTCC 3 cut(s) 470, 1589, 2217
Lsp1109I GCAGC 4 cut(s) 996, 2476, 2583, 2891
LweI GCATC 9 cut(s) 996, 1206, 1318, 1324, 1602, 1786, 2106, 2422, 2887
MaeI CTAG 8 cut(s) 546, 744, 894, 1013, 1665, 2495, 2675, 2916
MaeII ACGT 3 cut(s) 1263, 1541, 2787
MaeIII GTNAC 8 cut(s) 1036, 1642, 1673, 1712, 1945, 1983, 2309, 2542
MflI RGATCY 3 cut(s) 24, 2338, 2497
MhlI GDGCHC 2 cut(s) 1572, 2272
MlyI GAGTC 6 cut(s) 51, 210, 328, 800, 1103, 2762
MmeI TCCRAC 5 cut(s) 79, 219, 325, 1614, 1649
Mph1103I ATGCAT 5 cut(s) 643, 1411, 1459, 2099, 2396
MroI TCCGGA 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 668
MslI CAYNNNNRTG 4 cut(s) 270, 1043, 1107, 2063
MspA1I CMGCKG 2 cut(s) 2275, 2882
MspI CCGG 7 cut(s) 61, 83, 171, 620, 870, 1000, 1142
MspR9I CCNGG 4 cut(s) 871, 1157, 1198, 2949
Mva1269I GAATGC 6 cut(s) 643, 946, 1855, 2149, 2191, 2371
MvaI CCWGG 3 cut(s) 1157, 1198, 2949
MvnI CGCG 1 cut(s) 2601
MwoI GCNNNNNNNGC 7 cut(s) 171, 363, 461, 470, 647, 1463, 2515
NciI CCSGG 1 cut(s) 871
NcoI CCATGG 1 cut(s) 1573
NdeI CATATG 1 cut(s) 2390
NheI GCTAGC 1 cut(s) 743
NlaIV GGNNCC 3 cut(s) 630, 2229, 2832
NmeAIII GCCGAG 2 cut(s) 342, 382
NmuCI GTSAC 3 cut(s) 1036, 1642, 1673
NsiI ATGCAT 5 cut(s) 643, 1411, 1459, 2099, 2396
NspI RCATGY 2 cut(s) 1974, 2855
NspV TTCGAA 1 cut(s) 1815
OliI CACNNNNGTG 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 1974
PceI AGGCCT 2 cut(s) 455, 1027
PciI ACATGT 1 cut(s) 2851
PctI GAATGC 6 cut(s) 643, 946, 1855, 2149, 2191, 2371
PdmI GAANNNNTTC 1 cut(s) 668
PfeI GAWTC 5 cut(s) 669, 908, 1193, 1873, 2090
PflFI GACNNNGTC 1 cut(s) 1640
PkrI GCNGC 7 cut(s) 463, 1011, 2491, 2573, 2600, 2878, 2881
PleI GAGTC 6 cut(s) 51, 210, 327, 799, 1102, 2761
PpsI GAGTC 6 cut(s) 51, 210, 327, 799, 1102, 2761
Ppu21I YACGTR 1 cut(s) 1264
PpuMI RGGWCCY 1 cut(s) 255
PscI ACATGT 1 cut(s) 2851
PshBI ATTAAT 2 cut(s) 1553, 2457
Psp1406I AACGTT 1 cut(s) 2787
Psp5II RGGWCCY 1 cut(s) 255
Psp6I CCWGG 3 cut(s) 1155, 1196, 2947
PspEI GGTNACC 1 cut(s) 1642
PspFI CCCAGC 3 cut(s) 1799, 2761, 2882
PspGI CCWGG 3 cut(s) 1155, 1196, 2947
PspN4I GGNNCC 3 cut(s) 630, 2229, 2832
PspPI GGNCC 3 cut(s) 255, 2668, 2830
PspPPI RGGWCCY 1 cut(s) 255
PstI CTGCAG 1 cut(s) 2573
PstNI CAGNNNCTG 3 cut(s) 258, 337, 1343
PsuI RGATCY 3 cut(s) 24, 2338, 2497
PsyI GACNNNGTC 1 cut(s) 1640
PvuII CAGCTG 2 cut(s) 2275, 2882
RsaI GTAC 5 cut(s) 736, 866, 1262, 1268, 1775
RsaNI GTAC 5 cut(s) 735, 865, 1261, 1267, 1774
RseI CAYNNNNRTG 4 cut(s) 270, 1043, 1107, 2063
SatI GCNGC 7 cut(s) 462, 1010, 2490, 2572, 2599, 2877, 2880
Sau96I GGNCC 3 cut(s) 255, 2668, 2830
ScaI AGTACT 1 cut(s) 736
SchI GAGTC 6 cut(s) 51, 210, 328, 800, 1103, 2762
ScrFI CCNGG 4 cut(s) 871, 1157, 1198, 2949
SduI GDGCHC 2 cut(s) 1572, 2272
SfaNI GCATC 9 cut(s) 996, 1206, 1318, 1324, 1602, 1786, 2106, 2422, 2887
SfcI CTRYAG 2 cut(s) 912, 2569
SfuI TTCGAA 1 cut(s) 1815
SinI GGWCC 3 cut(s) 255, 2668, 2830
SmiMI CAYNNNNRTG 4 cut(s) 270, 1043, 1107, 2063
SmlI CTYRAG 3 cut(s) 191, 1376, 2750
SmoI CTYRAG 3 cut(s) 191, 1376, 2750
SpeI ACTAGT 1 cut(s) 545
SphI GCATGC 1 cut(s) 1974
SseBI AGGCCT 2 cut(s) 455, 1027
SsiI CCGC 4 cut(s) 461, 1331, 2599, 2877
SspI AATATT 1 cut(s) 2869
SspMI CTAG 8 cut(s) 546, 744, 894, 1013, 1665, 2495, 2675, 2916
StuI AGGCCT 2 cut(s) 455, 1027
StyD4I CCNGG 4 cut(s) 869, 1155, 1196, 2947
StyI CCWWGG 3 cut(s) 145, 632, 1573
TaaI ACNGT 4 cut(s) 1223, 1784, 1837, 2106
TaiI ACGT 3 cut(s) 1266, 1544, 2790
TaqI TCGA 5 cut(s) 603, 672, 1049, 1209, 1815
TatI WGTACW 3 cut(s) 734, 1266, 1773
TauI GCSGC 3 cut(s) 464, 2601, 2879
TfiI GAWTC 5 cut(s) 669, 908, 1193, 1873, 2090
TscAI CASTG 3 cut(s) 718, 1789, 2196
TseFI GTSAC 3 cut(s) 1036, 1642, 1673
TseI GCWGC 4 cut(s) 1009, 2489, 2571, 2879
Tsp45I GTSAC 3 cut(s) 1036, 1642, 1673
TspGWI ACGGA 3 cut(s) 429, 1598, 1602
TspRI CASTG 3 cut(s) 718, 1789, 2196
Tth111I GACNNNGTC 1 cut(s) 1640
VneI GTGCAC 1 cut(s) 1568
VpaK11BI GGWCC 3 cut(s) 255, 2668, 2830
VspI ATTAAT 2 cut(s) 1553, 2457
XapI RAATTY 8 cut(s) 609, 727, 1069, 1243, 1957, 2251, 2775, 2839
XceI RCATGY 2 cut(s) 1974, 2855
XcmI CCANNNNNNNNNTGG 1 cut(s) 1834
XmiI GTMKAC 1 cut(s) 1657
XmnI GAANNNNTTC 1 cut(s) 668
XspI CTAG 8 cut(s) 546, 744, 894, 1013, 1665, 2495, 2675, 2916
ZrmI AGTACT 1 cut(s) 736
Zsp2I ATGCAT 5 cut(s) 643, 1411, 1459, 2099, 2396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.