Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0012566

Physical Location & Seq

Genomic Coordinates Forward (+)

46554926 .. 46559548

Gene Structure 4623 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025539

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2571 bp

ATGGTGAAACTCTCTCGATGTACTGCCCCCCTTCTTTGTAATTTGGCCCTTGACATTGCTACTGTTTTGCGCTTAGTTGTGACTGAAGAAGATCATCTTCTGTTGGATATGGTTTCATTGGTTGGGCAAGGAGAAGCCGATGATAAACCATCTTTGAGTCTATTTGAGCAGAACCTTGTTATACAGATTAGTTTGGTTGACTTCTATGCAAGAACTGGGTGTGTGGACACTGCACGCCAAGTGATCGATAGAATTCCCCAACCGGACTTGGTTCCTTGGAATGCATTGATAGCTGGTTATTCGTCAAATGGATTCAATTGGGAAGCGTTAGAGGATGTCAGGGAGATTGTTTTCAGTGATCTGAGGCTGAATTTGAGTACTCTAGCTAGCATTGTTCCTGTTTGCACTCGTTTGGGGTGTGTTCGTGCTGGTAAGTCTCTTCATTGCTTTGCTGTCAAGTCTGGATACTTTTCGAATGATTTTCTGGTTCCTGCTTTGAGTTCAATGTACGCCGGGGATGGAGATATATGTGGTGCTAGAAAGTTGTTTGATTCTGTAGTGGAAAAGAATGTTGCTATTTGGAATGCCATGATTTCTGCATATACACAAAGGCAAGAGCCTGTGTTAGCTTTTGAGCTGTTCCGGTCGATGCTGCTAGTTGACATAAGGCCTAATTTGGTCACTTTCATGTCGATTATTCCATCATTTGAGAATTTCATTAGCCTTTCTTTCAGCGAGTCTCTTCATGCTTGTGTAGTTAAACATGGATCAGAAAATCAGCTTCCGGTATTAACTGCCCTGGTATCAATGTATGCCAAACTTGGTAGTATTGATGAATCCAGGTATCTTTTCAACCAGATGCAGAGTAAAAACCTTCTTTTGTGGAATTCAATGATTTCTGGGTACGTGTACAATGGGTTATGGGATTTAAGTCTGTGTTTGTTTCATGAAATGCAAGTTTCAAGATTTGATGCGGATGCAGTCTCTGTTATTAATATTCTTTCTGCTTGCTCCAAACTTGAGGCTGATATGTTGGGTAGGTCTGCCTATGCATTTAGTATAAGAACGGGTATCCACTCAAACATCAATCTGTCCAATGCATTGTTGGCATTTTACTCTGATTGTCATCGCCTCTCTTATTCTATTACTTTGTTTCACAAAATGCCTCTAAGGAATGGCATAACGTGGAATACATTAATTTCCAGTTGTGTGCACCATGGAGAAATGAACGGAGCAGTTGCCTTATACTACCAGATGCAGAAAGAGGGTTTCAAGTTGGACTTGGTCACCTTGACCAGTATACTGCCTAGTTGGAGTGACGAGGAAAACTTAGGGCAAGGAATGGCTTTTCATGGTTACGCTATAAAACGCGGGTTTGCTTCTGACATTTCTCTGCTTAACTCACTTATTAGCATGTACTTTAACTGTGGAGACCTTGATGCTGGGAGGTTGGTTTTTGAAACCATGCCCAAAAGAACTGTGGTGTCTTGGAATGCTATGATGACCGGCCTCAAGAATCATAATTTACAGAATGAGGTTATGGCCGTCTTCGGTCAAATGATAAAGGATAATCAGAGACCAAATTATGTAACTTTGCTAAATTTATTGCCTGCATGCTACTCCAAGTTACAGGGTAAGTCTATTCATGCTCTGGCAGTTAGAACAAGTATTCTCCATGAAACCCCTCTTCTTTCATCACTCATGTTTATGTATGCTAGATTTGACAACCATGATTCATGTATCTTACTGTTTAAAACGAGAAAAATGGAGGATATTTCTTTGTGGAATGCCATTATGTCAGTGCAAATACAAGCGAAAAGTAGCGAGAATGCAGTGGGATTTTTCAATAGCTTTCTCCAGATGAAATTGGAACCAGACAATTTGACCCTTCTGAATTTGGTCTCTTCATGTGCTCAGCTGAATAGCCTCACTCTTGCTAATTCCGTAATGGGTTACATCATTCGCAAGGGCTTTGACAAATATCTGGTTATTAGTAATGCACTTAACTCCTCAGCATTGCAGATGTGGTTGGTTGGTTATCCAGATACGTTAAGGGTTTTCATGAAGAAGCAAATTCAGTTCTTGGGTAGCCAGAAGAAGGTTCAAGGTGTAAAAAAATCTGCCACAGAAGCTCCGGGTGTTGATGTTGTTAGGCATAAGAAGACAAAGAGTGTTGATGGCACTGCTCTGATGGATGCTATTTTGGGTGCAATTTATGCTCAATCAAAGGCTTATGGGACGGCAAGACGGGTTAGAAACAAGAGTTCAACAAGCTTCTTAGCCCTTGCAATAGAATCTTCTGAAGCCTTATTGGTTGGGAATGCTCTCATGGTCGACGACAAGTTTCACACTGAGAGATCGCTCTCGTCCGGTAGTGGCCTTGGTATAGGCCTCGCCTACGCCGCTCGATCTGCTGCCGAAAGGGGATTGATGCTAACATTTAGGGAGATCTGCAGGGGTGCTGCTCGGAGTTGTTTGAAGGCTGGTTTTGGCTTCAGATTTCAAGTGAAAATCTCTCAGATGCGATGCCATGCGGTGTCAAATCGAAAGGCCTCGTTTGAAGCTTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 WEMBL

Protein Analysis

857

Amino Acids

94.9

Weight (kDa)

8.61

Isoelectric Point (pI)

39.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	189 - 233	2.2e-07	PPR repeat family
PPR_2	PF13041	394 - 435	4.9e-09	PPR repeat family
PPR	PF01535	394 - 424	5.8e-07	PPR repeat
FACT-Spt16_Nlob	PF14826	669 - 750	1.3e-12	FACT complex subunit SPT16 N-terminal lobe domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012566)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g15970
malus_domestica	MD02G1171400.v1.1
prunus_persica	Prupe.7G134000_v2.0.a1
pyrus_communis	pycom02g13810
rosa_chinensis	RchiOBHm_Chr2g0105571 RchiOBHm_Chr4g0419421
rosa_laevigata	RLG00000009251 RLG00000017437 RLG00000025539
rosa_multiflora	Rmu_co7991258.1_g000001 Rmu_sc0001051.1_g000009 Rmu_sc0006395.1_g000003
rosa_roxburghii	Rroxscaffold_2G00138120
rosa_rugosa	Rorug02G0128300
rosa_samantha	Rh2AG179900 Rh2BG189000 Rh2CG184200 Rh2DG185700
rosa_wichuraiana	Rw2G014070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	2405
AccI	GTMKAC	2 cut(s)	1300, 2334
AccII	CGCG	1 cut(s)	1371
AciI	CCGC	4 cut(s)	974, 1371, 2403, 2534
AclWI	GGATC	1 cut(s)	775
AcoI	YGGCCR	1 cut(s)	1542
AcsI	RAATTY	7 cut(s)	252, 370, 712, 886, 1600, 1894, 2073
AcuI	CTGAAG	3 cut(s)	105, 2322, 2479
AfaI	GTAC	6 cut(s)	22, 379, 509, 905, 911, 1418
AfiI	CCNNNNNNNGG	1 cut(s)	2098
AflIII	ACRYGT	1 cut(s)	906
AjnI	CCWGG	2 cut(s)	798, 839
Alw21I	GWGCWC	2 cut(s)	1215, 1915
Alw26I	GTCTC	6 cut(s)	441, 744, 988, 1425, 1570, 1906
Alw44I	GTGCAC	1 cut(s)	1211
AlwI	GGATC	1 cut(s)	775
AlwNI	CAGNNNCTG	1 cut(s)	986
AoxI	GGCC	7 cut(s)	45, 668, 1507, 1542, 2377, 2389, 2550
ApaLI	GTGCAC	1 cut(s)	1211
ApeKI	GCWGC	3 cut(s)	652, 2414, 2462
ApoI	RAATTY	7 cut(s)	252, 370, 712, 886, 1600, 1894, 2073
AseI	ATTAAT	2 cut(s)	993, 1196
AspLEI	GCGC	1 cut(s)	72
AspS9I	GGNCC	1 cut(s)	46
AsuC2I	CCSGG	2 cut(s)	514, 2136
AsuHPI	GGTGA	2 cut(s)	16, 1279
AsuII	TTCGAA	1 cut(s)	473
AsuNHI	GCTAGC	1 cut(s)	386
BaeGI	GKGCMC	1 cut(s)	1215
BbsI	GAAGAC	2 cut(s)	1540, 2168
Bbv12I	GWGCWC	2 cut(s)	1215, 1915
BbvCI	CCTCAGC	1 cut(s)	2011
BbvI	GCAGC	3 cut(s)	639, 2401, 2449
BccI	CCATC	5 cut(s)	157, 512, 709, 2171, 2185
BceAI	ACGGC	2 cut(s)	1529, 2256
BciT130I	CCWGG	2 cut(s)	800, 841
BciVI	GTATCC	2 cut(s)	458, 1082
BcnI	CCSGG	2 cut(s)	514, 2136
BcoDI	GTCTC	6 cut(s)	441, 744, 988, 1425, 1570, 1906
BfaI	CTAG	7 cut(s)	383, 387, 537, 656, 1308, 1716, 2569
BfmI	CTRYAG	2 cut(s)	555, 2452
BfuI	GTATCC	2 cut(s)	458, 1082
BglII	AGATCT	1 cut(s)	2448
BisI	GCNGC	4 cut(s)	653, 2403, 2415, 2463
BlpI	GCTNAGC	1 cut(s)	1914
BlsI	GCNGC	4 cut(s)	654, 2404, 2416, 2464
BmcAI	AGTACT	1 cut(s)	379
Bme1390I	CCNGG	4 cut(s)	514, 800, 841, 2136
BmgT120I	GGNCC	1 cut(s)	46
BmiI	GGNNCC	3 cut(s)	273, 489, 1872
BmrFI	CCNGG	4 cut(s)	514, 800, 841, 2136
BmrI	ACTGGG	1 cut(s)	225
BmtI	GCTAGC	1 cut(s)	390
BmuI	ACTGGG	1 cut(s)	225
BpiI	GAAGAC	2 cut(s)	1540, 2168
BpmI	CTGGAG	1 cut(s)	1841
Bpu10I	CCTNAGC	1 cut(s)	2011
Bpu1102I	GCTNAGC	1 cut(s)	1914
Bpu14I	TTCGAA	1 cut(s)	473
BpuEI	CTTGAG	2 cut(s)	1040, 1496
BpuMI	CCSGG	2 cut(s)	514, 2136
Bsa29I	ATCGAT	1 cut(s)	246
BsaAI	YACGTR	1 cut(s)	907
BsaBI	GATNNNNATC	1 cut(s)	1125
BsaI	GGTCTC	3 cut(s)	1425, 1570, 1906
BsaJI	CCNNGG	5 cut(s)	275, 513, 798, 1216, 2380
BsaWI	WCCGGW	4 cut(s)	262, 642, 784, 2369
BsaXI	ACNNNNNCTCC	2 cut(s)	2116, 2146
Bsc4I	CCNNNNNNNGG	1 cut(s)	2098
Bse118I	RCCGGY	1 cut(s)	1505
Bse1I	ACTGG	3 cut(s)	220, 1203, 1296
Bse3DI	GCAATG	3 cut(s)	54, 442, 2015
Bse8I	GATNNNNATC	1 cut(s)	1125
BseBI	CCWGG	2 cut(s)	800, 841
BseCI	ATCGAT	1 cut(s)	246
BseDI	CCNNGG	5 cut(s)	275, 513, 798, 1216, 2380
BseGI	GGATG	4 cut(s)	340, 523, 982, 2200
BseJI	GATNNNNATC	1 cut(s)	1125
BseLI	CCNNNNNNNGG	1 cut(s)	2098
BseMI	GCAATG	3 cut(s)	54, 442, 2015
BseMII	CTCAG	5 cut(s)	353, 1928, 2025, 2343, 2531
BseNI	ACTGG	3 cut(s)	220, 1203, 1296
BseRI	GAGGAG	1 cut(s)	1999
BseSI	GKGCMC	1 cut(s)	1215
BseXI	GCAGC	3 cut(s)	639, 2401, 2449
BseYI	CCCAGC	1 cut(s)	1442
BsgI	GTGCAG	1 cut(s)	216
Bsh1236I	CGCG	1 cut(s)	1371
Bsh1285I	CGRYCG	1 cut(s)	647
BshFI	GGCC	7 cut(s)	47, 670, 1509, 1544, 2379, 2391, 2552
BshVI	ATCGAT	1 cut(s)	246
BsiEI	CGRYCG	1 cut(s)	647
BsiHKAI	GWGCWC	2 cut(s)	1215, 1915
BsiSI	CCGG	7 cut(s)	263, 513, 643, 785, 1506, 2135, 2370
BslFI	GGGAC	1 cut(s)	2251
BslI	CCNNNNNNNGG	1 cut(s)	2098
BsmAI	GTCTC	6 cut(s)	441, 744, 988, 1425, 1570, 1906
BsmFI	GGGAC	1 cut(s)	2251
BsmI	GAATGC	6 cut(s)	286, 589, 1498, 1792, 1834, 2326
BsnI	GGCC	7 cut(s)	47, 670, 1509, 1544, 2379, 2391, 2552
Bso31I	GGTCTC	3 cut(s)	1425, 1570, 1906
Bsp119I	TTCGAA	1 cut(s)	473
Bsp1286I	GDGCHC	2 cut(s)	1215, 1915
Bsp1407I	TGTACA	1 cut(s)	909
Bsp143I	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
Bsp1720I	GCTNAGC	1 cut(s)	1914
Bsp19I	CCATGG	1 cut(s)	1216
BspACI	CCGC	4 cut(s)	974, 1371, 2403, 2534
BspANI	GGCC	7 cut(s)	47, 670, 1509, 1544, 2379, 2391, 2552
BspCNI	CTCAG	5 cut(s)	354, 1927, 2024, 2344, 2530
BspDI	ATCGAT	1 cut(s)	246
BspFNI	CGCG	1 cut(s)	1371
BspHI	TCATGA	2 cut(s)	946, 2061
BspLI	GGNNCC	3 cut(s)	273, 489, 1872
BspMAI	CTGCAG	1 cut(s)	2456
BspOI	GCTAGC	1 cut(s)	390
BspPI	GGATC	1 cut(s)	775
BspT104I	TTCGAA	1 cut(s)	473
BspTNI	GGTCTC	3 cut(s)	1425, 1570, 1906
BsrBI	CCGCTC	1 cut(s)	2405
BsrDI	GCAATG	3 cut(s)	54, 442, 2015
BsrFI	RCCGGY	1 cut(s)	1505
BsrGI	TGTACA	1 cut(s)	909
BsrI	ACTGG	3 cut(s)	220, 1203, 1296
BssAI	RCCGGY	1 cut(s)	1505
BssECI	CCNNGG	5 cut(s)	275, 513, 798, 1216, 2380
BssMI	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
BssNAI	GTATAC	1 cut(s)	1301
BssT1I	CCWWGG	3 cut(s)	275, 1216, 2380
Bst1107I	GTATAC	1 cut(s)	1301
Bst2UI	CCWGG	2 cut(s)	800, 841
Bst4CI	ACNGT	4 cut(s)	64, 1427, 1480, 1749
Bst6I	CTCTTC	4 cut(s)	444, 747, 1692, 1909
BstAPI	GCANNNNNTGC	1 cut(s)	2216
BstAUI	TGTACA	1 cut(s)	909
BstBAI	YACGTR	1 cut(s)	907
BstBI	TTCGAA	1 cut(s)	473
BstC8I	GCNNGC	5 cut(s)	235, 388, 1009, 1611, 1615
BstDEI	CTNAG	9 cut(s)	73, 362, 1169, 1330, 1914, 2011, 2278, 2352, 2517
BstDSI	CCRYGG	1 cut(s)	1216
BstEII	GGTNACC	1 cut(s)	1285
BstF5I	GGATG	4 cut(s)	340, 523, 982, 2200
BstFNI	CGCG	1 cut(s)	1371
BstHHI	GCGC	1 cut(s)	72
BstKTI	GATC	7 cut(s)	94, 246, 361, 770, 2360, 2411, 2451
BstMAI	GTCTC	6 cut(s)	441, 744, 988, 1425, 1570, 1906
BstMBI	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
BstMCI	CGRYCG	1 cut(s)	647
BstMWI	GCNNNNNNNGC	6 cut(s)	290, 1106, 2129, 2216, 2402, 2411
BstNI	CCWGG	2 cut(s)	800, 841
BstNSI	RCATGY	2 cut(s)	1417, 1617
BstPI	GGTNACC	1 cut(s)	1285
BstSCI	CCNGG	4 cut(s)	512, 798, 839, 2134
BstSFI	CTRYAG	2 cut(s)	555, 2452
BstSLI	GKGCMC	1 cut(s)	1215
BstUI	CGCG	1 cut(s)	1371
BstV1I	GCAGC	3 cut(s)	639, 2401, 2449
BstV2I	GAAGAC	2 cut(s)	1540, 2168
BstX2I	RGATCY	1 cut(s)	2448
BstYI	RGATCY	1 cut(s)	2448
BstZ17I	GTATAC	1 cut(s)	1301
Bsu15I	ATCGAT	1 cut(s)	246
BsuI	GTATCC	2 cut(s)	458, 1082
BsuRI	GGCC	7 cut(s)	47, 670, 1509, 1544, 2379, 2391, 2552
BsuTUI	ATCGAT	1 cut(s)	246
BtgI	CCRYGG	1 cut(s)	1216
BtgZI	GCGATG	2 cut(s)	1112, 2539
BtsCI	GGATG	4 cut(s)	340, 523, 982, 2200
BtsI	GCAGTG	3 cut(s)	228, 1839, 2181
BtsIMutI	CAGTG	6 cut(s)	228, 361, 1806, 1839, 2181, 2349
Cac8I	GCNNGC	5 cut(s)	235, 388, 1009, 1611, 1615
CaiI	CAGNNNCTG	1 cut(s)	986
CciI	TCATGA	2 cut(s)	946, 2061
CfoI	GCGC	1 cut(s)	72
Cfr10I	RCCGGY	1 cut(s)	1505
Cfr13I	GGNCC	1 cut(s)	46
ClaI	ATCGAT	1 cut(s)	246
Csp6I	GTAC	6 cut(s)	21, 378, 508, 904, 910, 1417
CviQI	GTAC	6 cut(s)	21, 378, 508, 904, 910, 1417
DdeI	CTNAG	9 cut(s)	73, 362, 1169, 1330, 1914, 2011, 2278, 2352, 2517
DpnI	GATC	7 cut(s)	93, 245, 360, 769, 2359, 2410, 2450
DpnII	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
DraI	TTTAAA	1 cut(s)	1753
EaeI	YGGCCR	1 cut(s)	1542
Eam1104I	CTCTTC	4 cut(s)	444, 747, 1692, 1909
EarI	CTCTTC	4 cut(s)	444, 747, 1692, 1909
Eco130I	CCWWGG	3 cut(s)	275, 1216, 2380
Eco147I	AGGCCT	3 cut(s)	670, 2391, 2552
Eco31I	GGTCTC	3 cut(s)	1425, 1570, 1906
Eco57I	CTGAAG	3 cut(s)	105, 2322, 2479
Eco91I	GGTNACC	1 cut(s)	1285
EcoO65I	GGTNACC	1 cut(s)	1285
EcoRI	GAATTC	2 cut(s)	252, 886
EcoRII	CCWGG	2 cut(s)	798, 839
EcoT14I	CCWWGG	3 cut(s)	275, 1216, 2380
EcoT22I	ATGCAT	3 cut(s)	286, 1054, 1102
ErhI	CCWWGG	3 cut(s)	275, 1216, 2380
FalI	AAGNNNNNCTT	4 cut(s)	81, 113, 1265, 1297
FaqI	GGGAC	1 cut(s)	2251
FauI	CCCGC	1 cut(s)	1364
FblI	GTMKAC	2 cut(s)	1300, 2334
Fnu4HI	GCNGC	4 cut(s)	653, 2403, 2415, 2463
FokI	GGATG	4 cut(s)	347, 530, 989, 2207
Fsp4HI	GCNGC	4 cut(s)	653, 2403, 2415, 2463
FspBI	CTAG	7 cut(s)	383, 387, 537, 656, 1308, 1716, 2569
GlaI	GCGC	1 cut(s)	71
GluI	GCNGC	4 cut(s)	653, 2403, 2415, 2463
GsaI	CCCAGC	1 cut(s)	1446
GsuI	CTGGAG	1 cut(s)	1841
HaeIII	GGCC	7 cut(s)	47, 670, 1509, 1544, 2379, 2391, 2552
HapII	CCGG	7 cut(s)	263, 513, 643, 785, 1506, 2135, 2370
HhaI	GCGC	1 cut(s)	72
Hin6I	GCGC	1 cut(s)	70
HinP1I	GCGC	1 cut(s)	70
HincII	GTYRAC	3 cut(s)	199, 661, 2335
HindII	GTYRAC	3 cut(s)	199, 661, 2335
HindIII	AAGCTT	2 cut(s)	2272, 2562
HinfI	GANTC	8 cut(s)	157, 312, 551, 737, 836, 1516, 1733, 2294
HpaII	CCGG	7 cut(s)	263, 513, 643, 785, 1506, 2135, 2370
HphI	GGTGA	2 cut(s)	16, 1279
Hpy166II	GTNNAC	7 cut(s)	199, 226, 661, 910, 1213, 1301, 2335
Hpy188III	TCNNGA	8 cut(s)	15, 462, 947, 963, 1513, 1858, 2042, 2062
Hpy8I	GTNNAC	7 cut(s)	199, 226, 661, 910, 1213, 1301, 2335
Hpy99I	CGWCG	1 cut(s)	2339
HpyAV	CCTTC	5 cut(s)	41, 884, 1898, 2092, 2473
HpyCH4III	ACNGT	4 cut(s)	64, 1427, 1480, 1749
HpyCH4IV	ACGT	3 cut(s)	906, 1184, 2048
HpyF10VI	GCNNNNNNNGC	6 cut(s)	290, 1106, 2129, 2216, 2402, 2411
HpyF3I	CTNAG	9 cut(s)	73, 362, 1169, 1330, 1914, 2011, 2278, 2352, 2517
HpySE526I	ACGT	3 cut(s)	906, 1184, 2048
HspAI	GCGC	1 cut(s)	70
Kzo9I	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
LmnI	GCTCC	3 cut(s)	1016, 1232, 2137
Lsp1109I	GCAGC	3 cut(s)	639, 2401, 2449
MaeI	CTAG	7 cut(s)	383, 387, 537, 656, 1308, 1716, 2569
MaeII	ACGT	3 cut(s)	906, 1184, 2048
MaeIII	GTNAC	8 cut(s)	79, 679, 1285, 1316, 1355, 1588, 1626, 1952
MalI	GATC	7 cut(s)	93, 245, 360, 769, 2359, 2410, 2450
MbiI	CCGCTC	1 cut(s)	2405
MboI	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
MfeI	CAATTG	1 cut(s)	316
MflI	RGATCY	1 cut(s)	2448
MhlI	GDGCHC	2 cut(s)	1215, 1915
MlyI	GAGTC	2 cut(s)	166, 746
MmeI	TCCRAC	3 cut(s)	84, 1257, 1292
Mph1103I	ATGCAT	3 cut(s)	286, 1054, 1102
MslI	CAYNNNNRTG	3 cut(s)	686, 750, 1706
MspA1I	CMGCKG	1 cut(s)	1918
MspI	CCGG	7 cut(s)	263, 513, 643, 785, 1506, 2135, 2370
MspR9I	CCNGG	4 cut(s)	514, 800, 841, 2136
MunI	CAATTG	1 cut(s)	316
Mva1269I	GAATGC	6 cut(s)	286, 589, 1498, 1792, 1834, 2326
MvaI	CCWGG	2 cut(s)	800, 841
MvnI	CGCG	1 cut(s)	1371
MwoI	GCNNNNNNNGC	6 cut(s)	290, 1106, 2129, 2216, 2402, 2411
NciI	CCSGG	2 cut(s)	514, 2136
NcoI	CCATGG	1 cut(s)	1216
NdeII	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
NheI	GCTAGC	1 cut(s)	386
NlaIV	GGNNCC	3 cut(s)	273, 489, 1872
NmuCI	GTSAC	4 cut(s)	79, 679, 1285, 1316
NsiI	ATGCAT	3 cut(s)	286, 1054, 1102
NspI	RCATGY	2 cut(s)	1417, 1617
NspV	TTCGAA	1 cut(s)	473
PaeI	GCATGC	1 cut(s)	1617
PagI	TCATGA	2 cut(s)	946, 2061
PceI	AGGCCT	3 cut(s)	670, 2391, 2552
PctI	GAATGC	6 cut(s)	286, 589, 1498, 1792, 1834, 2326
PfeI	GAWTC	6 cut(s)	312, 551, 836, 1516, 1733, 2294
PflFI	GACNNNGTC	1 cut(s)	1283
PkrI	GCNGC	4 cut(s)	654, 2404, 2416, 2464
PleI	GAGTC	2 cut(s)	165, 745
PpsI	GAGTC	2 cut(s)	165, 745
Ppu21I	YACGTR	1 cut(s)	907
PshBI	ATTAAT	2 cut(s)	993, 1196
Psp6I	CCWGG	2 cut(s)	798, 839
PspEI	GGTNACC	1 cut(s)	1285
PspFI	CCCAGC	1 cut(s)	1442
PspGI	CCWGG	2 cut(s)	798, 839
PspN4I	GGNNCC	3 cut(s)	273, 489, 1872
PspPI	GGNCC	1 cut(s)	46
PstI	CTGCAG	1 cut(s)	2456
PstNI	CAGNNNCTG	1 cut(s)	986
PsuI	RGATCY	1 cut(s)	2448
PsyI	GACNNNGTC	1 cut(s)	1283
PvuII	CAGCTG	1 cut(s)	1918
RsaI	GTAC	6 cut(s)	22, 379, 509, 905, 911, 1418
RsaNI	GTAC	6 cut(s)	21, 378, 508, 904, 910, 1417
RseI	CAYNNNNRTG	3 cut(s)	686, 750, 1706
SalI	GTCGAC	1 cut(s)	2333
SatI	GCNGC	4 cut(s)	653, 2403, 2415, 2463
Sau3AI	GATC	7 cut(s)	91, 243, 358, 767, 2357, 2408, 2448
Sau96I	GGNCC	1 cut(s)	46
ScaI	AGTACT	1 cut(s)	379
SchI	GAGTC	2 cut(s)	166, 746
ScrFI	CCNGG	4 cut(s)	514, 800, 841, 2136
SduI	GDGCHC	2 cut(s)	1215, 1915
SfcI	CTRYAG	2 cut(s)	555, 2452
SfuI	TTCGAA	1 cut(s)	473
SmiMI	CAYNNNNRTG	3 cut(s)	686, 750, 1706
SmlI	CTYRAG	2 cut(s)	1019, 1511
SmoI	CTYRAG	2 cut(s)	1019, 1511
SphI	GCATGC	1 cut(s)	1617
SseBI	AGGCCT	3 cut(s)	670, 2391, 2552
SsiI	CCGC	4 cut(s)	974, 1371, 2403, 2534
SspI	AATATT	1 cut(s)	997
SspMI	CTAG	7 cut(s)	383, 387, 537, 656, 1308, 1716, 2569
StuI	AGGCCT	3 cut(s)	670, 2391, 2552
StyD4I	CCNGG	4 cut(s)	512, 798, 839, 2134
StyI	CCWWGG	3 cut(s)	275, 1216, 2380
TaaI	ACNGT	4 cut(s)	64, 1427, 1480, 1749
TaiI	ACGT	3 cut(s)	909, 1187, 2051
TaqI	TCGA	8 cut(s)	16, 246, 473, 647, 692, 2334, 2407, 2545
TaqII	GACCGA	1 cut(s)	1541
TatI	WGTACW	4 cut(s)	20, 377, 909, 1416
TauI	GCSGC	1 cut(s)	2405
TfiI	GAWTC	6 cut(s)	312, 551, 836, 1516, 1733, 2294
TscAI	CASTG	6 cut(s)	235, 361, 1806, 1839, 2188, 2356
TseFI	GTSAC	4 cut(s)	79, 679, 1285, 1316
TseI	GCWGC	3 cut(s)	652, 2414, 2462
Tsp45I	GTSAC	4 cut(s)	79, 679, 1285, 1316
TspGWI	ACGGA	2 cut(s)	1245, 1933
TspRI	CASTG	6 cut(s)	235, 361, 1806, 1839, 2188, 2356
Tth111I	GACNNNGTC	1 cut(s)	1283
VneI	GTGCAC	1 cut(s)	1211
VspI	ATTAAT	2 cut(s)	993, 1196
XapI	RAATTY	7 cut(s)	252, 370, 712, 886, 1600, 1894, 2073
XceI	RCATGY	2 cut(s)	1417, 1617
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1102, 1477
XmiI	GTMKAC	2 cut(s)	1300, 2334
XspI	CTAG	7 cut(s)	383, 387, 537, 656, 1308, 1716, 2569
ZrmI	AGTACT	1 cut(s)	379
Zsp2I	ATGCAT	3 cut(s)	286, 1054, 1102

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.