RLG00000017437

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012566
Physical Location & Seq
Reverse (-)
16729818 .. 16732754
2937 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017437

Sequence Viewer

Length: 2937 bp
ATGGGATTGTTTTTCCGACCCCGGAACACTCTTTCTTCTCCTCCGGCGATAACCTCCTTTGTCAGACACAAACACCATTCAATCAATCCCAAAGCCTCACACTTCCTTGGTCTGCTCAATCTCTGCCGAACCGGCGAGGACTTGAAACCCCTCAAGTCCTTTCTCTCTGCTCATGGACTCATCAACCATGTCTCTTTGTTAGAACAATTTTTCAGGTCCTGTTTTCATCTGGGTGCTTCACATTTAGCTCTCTTTACATTCCAACAAATCAAAAGGCCAAGCCTTGATTTGCAGAACCTGTTGATCAGGTGTCTTTGCAATCATGGCTTATACCAAGACCTTTTATGTGTGTATATGAAATCTCGGCTTTCGGGTTGTCCTTCTGATGATTTCACATTTCCGTTTGTGATCAAGGCCTGTGCGGCTTTGGAAGCTGTTAAGATTGGTAGAGAGGTTCATGGTGTTGTGTTCAGAACTGGGTTTGAGCAGAACCTTGTTATACAGACTAGTTTGGTTGATTTCTATGCAAGAACTGGGTTTGTGGAAACTGCACGCCAAGTGATCGATAGAATTCCCCAACCGGACTTGGTTCCTTGGAATGCATTGATAGCTGGTTATTCGTCGAATGGATGGGATTGGGAAGCGTTAGAGGTATTCAGGGAGATTGTTTTCAGTGATCTGAGGCCGAATTTGAGTACTCTAGCTAGCATTGTTCCTGTTTGCACTCGTTTGGGGTGCGTTCGTGCTGGTAAGTCTCTTCATTGCTTTGCTGTCAAGTCTGGATACTTCTCGAATGATTTTCTGGTTCCTGCTTTGATTTCAATGTACGCCGGGGATGGGGATTTATGTGGTGCTAGAAACTTGTTTGATTCTGTAGTGGAAAAGAATGTTGCTGTTTGGAATGCCATGATTTCTGCATATACACAGAGGCAAGAGCCTGTGTTAGCTTTTGAGTTGTTCCGCTGGATGCTGCTAGATGACATAAGGCCTAATTTGGTCACTTTTGTGTCGATTATTCCCTCATTTGAGAATTTCATTAGCCTTTCTTTTGGCGAGTCTCTTCATGCTTGTGTAGTTAAACATGGATCAGAAAATCAGCTTCCGGTATTGACTGCCCTGGTATCAATGTATGCCAAACTTGGTTGTATTGATGAATCCAGGTATCTTTTCGACCAGATGCAAAGTAAAAACCTTCTTTTGTGGAATTCAATGATTTCTGGGTACGTGTACAATGGGTTATGGGATTTAAGTCTGGGTTTGTTTCGTGAAATGCAAGTTTCAAGATTTGATGCAGATGCAGTCTCTGTTATTAGTATTCTTTCTGCTTGCTCCAAACTTGAGGCTGATATGTTGGGTAGGTCTGCCCATGCATTTAGTATAAGAAAGGGTATCCACTCAAACATCAATCTGTCAAATGCATTGTTGGCATTTTACTCTGATTGTCATCGTCTCTCTTATTCTGTTACTTTGTTTCACAAAATGCCTCTAAGAAATGGCATAACGTGGAATACATTAATTTCCAGTTGTGTGCACCATGGAGAAATGGACGGAGCAGTTGCCTTATACTACCAGATGCAGAAAGAGGGTTTCAAGTTGGACTTGGTCACCTTGACCAGTATACTCCCTAGTTGGAGTGATGAGGAAAACTTGGGGCAAGGAATGGCTTTTCATGGTTATGCTATAAAACATGGGTTTGCTTCTGACATTTCTCTGCTTAACTCACTTATTAGCATGTACTTTAACTGTGGAGACCTTGATGCTGGGAGGTTGGTTTTCGAAACCATGCCCAAAAGAACTGTGGTGTCTTGGAATGCTCTGATGACTGGCTTCAAGAATCATAATATACAGAATGAGGTTATGGCCGTCTTTGGTCAAATGATAAAGGATGATCAGAGACCAAATTATGTAACTTTGCTAAATTTATTGCCTGCATGCTACTCCCAGTTACAGGGTAAGTCTATTCATGCTCTGGCAGTTAGAACAAGTATTCTCCATGAAACCCCTCTTCTTTCATCACTCATGTTTATGTATGCCAGATTTGACAACCATGATTCATGTATCTTACTCTTTAAAACGAGAAAAATGGAGGATATTTCTTTGTGGAATGCCATTATGTCGGTGCAAATACAAGCAAAAAGTAGCGAGAATGCAGTAGGATTTTTCAATAGCTTGCTCCAGATGAAATTGGAACCAGACAATTTGACCCTCCTGAATTTGGTCTCTTCATGTGCTCAACTGAATAGCCTCACTCTTGCTAATTCTGTAATGGGTTACATCATTCGCAAGGGCTTTGACAAAGATCTGGTTATTAGTAATGCACTAATAGATTTGCATTCAAGATGTGGAAACATATGCATTGCGAGAAAGCTGTTTGATGGGTTGGTAAAGAGAGATGCTGTGTCTTGGAGTGTGATGATTAATGGGTACGGCTTGAATGGGAATGGCGAACCTGCCCTGGATCTTTTCTTGCAGATGAAGCTATCAGGGATAGCACCTAATGGGATTACTTATTTAAGTGTTTTATCAGCCTGCAGCCATTCTGGATTGGTTGAGCAAGGCCGCGCCGTGTTCAAATCTATGGCAGAACATGGGATAACACCCCAAATGAAGCACTATGCTTGCATGGTGGACCTTCTAGGAAGAACGGGTAATTTGACCGAGGCTTATGACATTGTTCGGGAACTACCCTGCAAACCTTCAACAAGCCTACTCGAGTCTCTGCTGGGTGCTTGTAGAATTCATGGGAATGTTGAACTTGGAGTGAATATTGGTGGGTTGCTCTCTGAATTGGACCCTGAAAATTCAAGACCACATGTAATGCTTCATAATATTTATGCAGCAGCTGGTAGGTGGACTGATGCTGATAGAGTCAGGTCTAGGATTGAAGAGAGAAGTTTGAGAAAAGTTCCTGGTTTCAGTCTTCTAATGGGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 WEMBL

Protein Analysis

979

Amino Acids

109.02

Weight (kDa)

7.55

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 295 - 340 2.1e-08 PPR repeat family
PPR PF01535 401 - 427 6.4e-06 PPR repeat
PPR_2 PF13041 500 - 541 4.6e-09 PPR repeat family
PPR PF01535 500 - 530 1.5e-07 PPR repeat
PPR_2 PF13041 798 - 845 8.4e-10 PPR repeat family
PPR PF01535 800 - 830 3e-06 PPR repeat
E_motif PF20431 914 - 973 1.3e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2458
AccI GTMKAC 1 cut(s) 1618
AccII CGCG 1 cut(s) 2562
AciI CCGC 3 cut(s) 422, 961, 2560
AclWI GGATC 2 cut(s) 1093, 2466
AcoI YGGCCR 1 cut(s) 1860
AcsI RAATTY 8 cut(s) 570, 688, 1030, 1204, 1918, 2212, 2736, 2800
AfaI GTAC 6 cut(s) 697, 827, 1223, 1229, 1736, 2426
AfiI CCNNNNNNNGG 3 cut(s) 837, 1948, 2215
AflIII ACRYGT 2 cut(s) 1224, 2812
AhlI ACTAGT 1 cut(s) 506
AjnI CCWGG 4 cut(s) 1116, 1157, 2454, 2908
AleI CACNNNNGTG 1 cut(s) 1004
Alw21I GWGCWC 2 cut(s) 1533, 2233
Alw26I GTCTC 9 cut(s) 196, 759, 1062, 1306, 1454, 1743, 1888, 2224, 2721
Alw44I GTGCAC 1 cut(s) 1529
AlwI GGATC 2 cut(s) 1093, 2466
AlwNI CAGNNNCTG 4 cut(s) 219, 298, 1304, 2843
Ama87I CYCGRG 1 cut(s) 2711
AoxI GGCC 6 cut(s) 275, 414, 683, 986, 1860, 2557
ApaLI GTGCAC 1 cut(s) 1529
ApeKI GCWGC 4 cut(s) 970, 2532, 2837, 2840
ApoI RAATTY 8 cut(s) 570, 688, 1030, 1204, 1918, 2212, 2736, 2800
AseI ATTAAT 2 cut(s) 1514, 2418
AspLEI GCGC 1 cut(s) 2564
AspS9I GGNCC 3 cut(s) 216, 2629, 2791
AsuC2I CCSGG 2 cut(s) 22, 832
AsuHPI GGTGA 1 cut(s) 1597
AsuII TTCGAA 1 cut(s) 1776
AsuNHI GCTAGC 1 cut(s) 704
AvaI CYCGRG 1 cut(s) 2711
AvaII GGWCC 3 cut(s) 216, 2629, 2791
BaeGI GKGCMC 1 cut(s) 1533
BbsI GAAGAC 1 cut(s) 2912
Bbv12I GWGCWC 2 cut(s) 1533, 2233
BbvI GCAGC 4 cut(s) 957, 2544, 2849, 2852
BccI CCATC 3 cut(s) 624, 830, 2369
BceAI ACGGC 3 cut(s) 1847, 2443, 2549
BciT130I CCWGG 4 cut(s) 1118, 1159, 2456, 2910
BciVI GTATCC 2 cut(s) 776, 1400
BclI TGATCA 3 cut(s) 303, 408, 1888
BcnI CCSGG 2 cut(s) 22, 832
BcoDI GTCTC 9 cut(s) 196, 759, 1062, 1306, 1454, 1743, 1888, 2224, 2721
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 8 cut(s) 507, 701, 705, 855, 974, 1626, 2636, 2877
BfmI CTRYAG 2 cut(s) 873, 2530
BfuAI ACCTGC 1 cut(s) 2458
BfuI GTATCC 2 cut(s) 776, 1400
BglI GCCNNNNNGGC 2 cut(s) 132, 422
BglII AGATCT 1 cut(s) 2299
BisI GCNGC 6 cut(s) 423, 971, 2533, 2560, 2838, 2841
BlsI GCNGC 6 cut(s) 424, 972, 2534, 2561, 2839, 2842
BmcAI AGTACT 1 cut(s) 697
Bme1390I CCNGG 6 cut(s) 22, 832, 1118, 1159, 2456, 2910
Bme18I GGWCC 3 cut(s) 216, 2629, 2791
BmeT110I CYCGRG 1 cut(s) 2711
BmgT120I GGNCC 3 cut(s) 216, 2629, 2791
BmiI GGNNCC 4 cut(s) 591, 807, 2190, 2793
BmrFI CCNGG 6 cut(s) 22, 832, 1118, 1159, 2456, 2910
BmrI ACTGGG 3 cut(s) 486, 543, 1936
BmsI GCATC 8 cut(s) 957, 1167, 1279, 1285, 1563, 1747, 2383, 2848
BmtI GCTAGC 1 cut(s) 708
BmuI ACTGGG 3 cut(s) 486, 543, 1936
BpiI GAAGAC 1 cut(s) 2912
BpmI CTGGAG 1 cut(s) 2159
Bpu14I TTCGAA 1 cut(s) 1776
BpuEI CTTGAG 2 cut(s) 137, 1358
BpuMI CCSGG 2 cut(s) 22, 832
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 1225
BsaBI GATNNNNATC 1 cut(s) 1443
BsaI GGTCTC 3 cut(s) 1743, 1888, 2224
BsaJI CCNNGG 8 cut(s) 20, 106, 593, 831, 1116, 1534, 2454, 2658
BsaWI WCCGGW 2 cut(s) 580, 1102
Bsc4I CCNNNNNNNGG 3 cut(s) 837, 1948, 2215
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 6 cut(s) 481, 538, 1521, 1614, 1828, 1942
Bse3DI GCAATG 2 cut(s) 760, 2355
Bse8I GATNNNNATC 1 cut(s) 1443
BseBI CCWGG 4 cut(s) 1118, 1159, 2456, 2910
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 8 cut(s) 20, 106, 593, 831, 1116, 1534, 2454, 2658
BseGI GGATG 4 cut(s) 635, 841, 972, 1891
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 3 cut(s) 837, 1948, 2215
BseMI GCAATG 2 cut(s) 760, 2355
BseMII CTCAG 1 cut(s) 671
BseNI ACTGG 6 cut(s) 481, 538, 1521, 1614, 1828, 1942
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 1533
BseXI GCAGC 4 cut(s) 957, 2544, 2849, 2852
BseYI CCCAGC 2 cut(s) 1760, 2722
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 2562
BshFI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 1533, 2233
BsiHKCI CYCGRG 1 cut(s) 2711
BsiSI CCGG 6 cut(s) 22, 44, 132, 581, 831, 1103
BslI CCNNNNNNNGG 3 cut(s) 837, 1948, 2215
BsmAI GTCTC 9 cut(s) 196, 759, 1062, 1306, 1454, 1743, 1888, 2224, 2721
BsmBI CGTCTC 1 cut(s) 1454
BsmI GAATGC 6 cut(s) 604, 907, 1816, 2110, 2152, 2332
BsnI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
Bso31I GGTCTC 3 cut(s) 1743, 1888, 2224
BsoBI CYCGRG 1 cut(s) 2711
Bsp119I TTCGAA 1 cut(s) 1776
Bsp1286I GDGCHC 2 cut(s) 1533, 2233
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
Bsp19I CCATGG 1 cut(s) 1534
BspACI CCGC 3 cut(s) 422, 961, 2560
BspANI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
BspCNI CTCAG 1 cut(s) 672
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 1 cut(s) 2562
BspLI GGNNCC 4 cut(s) 591, 807, 2190, 2793
BspMAI CTGCAG 1 cut(s) 2534
BspMI ACCTGC 1 cut(s) 2458
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 2 cut(s) 1093, 2466
BspT104I TTCGAA 1 cut(s) 1776
BspTNI GGTCTC 3 cut(s) 1743, 1888, 2224
BsrDI GCAATG 2 cut(s) 760, 2355
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 6 cut(s) 481, 538, 1521, 1614, 1828, 1942
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 8 cut(s) 20, 106, 593, 831, 1116, 1534, 2454, 2658
BssMI GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
BssNAI GTATAC 1 cut(s) 1619
BssT1I CCWWGG 3 cut(s) 106, 593, 1534
Bst1107I GTATAC 1 cut(s) 1619
Bst2UI CCWGG 4 cut(s) 1118, 1159, 2456, 2910
Bst4CI ACNGT 2 cut(s) 1745, 1798
Bst6I CTCTTC 5 cut(s) 762, 1065, 2010, 2227, 2880
BstAUI TGTACA 1 cut(s) 1227
BstBAI YACGTR 1 cut(s) 1225
BstBI TTCGAA 1 cut(s) 1776
BstC8I GCNNGC 8 cut(s) 553, 706, 1327, 1929, 1933, 2171, 2530, 2620
BstDEI CTNAG 2 cut(s) 680, 1487
BstDSI CCRYGG 1 cut(s) 1534
BstEII GGTNACC 1 cut(s) 1603
BstF5I GGATG 4 cut(s) 635, 841, 972, 1891
BstFNI CGCG 1 cut(s) 2562
BstHHI GCGC 1 cut(s) 2564
BstKTI GATC 8 cut(s) 306, 411, 564, 679, 1088, 1891, 2302, 2461
BstMAI GTCTC 9 cut(s) 196, 759, 1062, 1306, 1454, 1743, 1888, 2224, 2721
BstMBI GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
BstMWI GCNNNNNNNGC 7 cut(s) 132, 324, 422, 431, 608, 1424, 2476
BstNI CCWGG 4 cut(s) 1118, 1159, 2456, 2910
BstNSI RCATGY 3 cut(s) 1735, 1935, 2816
BstPI GGTNACC 1 cut(s) 1603
BstSCI CCNGG 6 cut(s) 20, 830, 1116, 1157, 2454, 2908
BstSFI CTRYAG 2 cut(s) 873, 2530
BstSLI GKGCMC 1 cut(s) 1533
BstUI CGCG 1 cut(s) 2562
BstV1I GCAGC 4 cut(s) 957, 2544, 2849, 2852
BstV2I GAAGAC 1 cut(s) 2912
BstX2I RGATCY 2 cut(s) 2299, 2458
BstYI RGATCY 2 cut(s) 2299, 2458
BstZ17I GTATAC 1 cut(s) 1619
Bsu15I ATCGAT 1 cut(s) 564
BsuI GTATCC 2 cut(s) 776, 1400
BsuRI GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 1534
BtsCI GGATG 4 cut(s) 635, 841, 972, 1891
BtsIMutI CAGTG 1 cut(s) 679
BveI ACCTGC 1 cut(s) 2458
Cac8I GCNNGC 8 cut(s) 553, 706, 1327, 1929, 1933, 2171, 2530, 2620
CaiI CAGNNNCTG 4 cut(s) 219, 298, 1304, 2843
CfoI GCGC 1 cut(s) 2564
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 216, 2629, 2791
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 6 cut(s) 696, 826, 1222, 1228, 1735, 2425
CviQI GTAC 6 cut(s) 696, 826, 1222, 1228, 1735, 2425
DdeI CTNAG 2 cut(s) 680, 1487
DpnI GATC 8 cut(s) 305, 410, 563, 678, 1087, 1890, 2301, 2460
DpnII GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
DraI TTTAAA 1 cut(s) 2071
EaeI YGGCCR 1 cut(s) 1860
Eam1104I CTCTTC 5 cut(s) 762, 1065, 2010, 2227, 2880
EarI CTCTTC 5 cut(s) 762, 1065, 2010, 2227, 2880
Eco130I CCWWGG 3 cut(s) 106, 593, 1534
Eco147I AGGCCT 2 cut(s) 416, 988
Eco31I GGTCTC 3 cut(s) 1743, 1888, 2224
Eco47I GGWCC 3 cut(s) 216, 2629, 2791
Eco88I CYCGRG 1 cut(s) 2711
Eco91I GGTNACC 1 cut(s) 1603
EcoO109I RGGNCCY 1 cut(s) 216
EcoO65I GGTNACC 1 cut(s) 1603
EcoRI GAATTC 3 cut(s) 570, 1204, 2736
EcoRII CCWGG 4 cut(s) 1116, 1157, 2454, 2908
EcoT14I CCWWGG 3 cut(s) 106, 593, 1534
EcoT22I ATGCAT 4 cut(s) 604, 1372, 1420, 2357
ErhI CCWWGG 3 cut(s) 106, 593, 1534
Esp3I CGTCTC 1 cut(s) 1454
FalI AAGNNNNNCTT 2 cut(s) 1583, 1615
FauNDI CATATG 1 cut(s) 2351
FbaI TGATCA 3 cut(s) 303, 408, 1888
FblI GTMKAC 1 cut(s) 1618
Fnu4HI GCNGC 6 cut(s) 423, 971, 2533, 2560, 2838, 2841
FokI GGATG 4 cut(s) 642, 848, 979, 1898
Fsp4HI GCNGC 6 cut(s) 423, 971, 2533, 2560, 2838, 2841
FspBI CTAG 8 cut(s) 507, 701, 705, 855, 974, 1626, 2636, 2877
GlaI GCGC 1 cut(s) 2563
GluI GCNGC 6 cut(s) 423, 971, 2533, 2560, 2838, 2841
GsaI CCCAGC 2 cut(s) 1764, 2726
GsuI CTGGAG 1 cut(s) 2159
HaeIII GGCC 6 cut(s) 277, 416, 685, 988, 1862, 2559
HapII CCGG 6 cut(s) 22, 44, 132, 581, 831, 1103
HhaI GCGC 1 cut(s) 2564
Hin6I GCGC 1 cut(s) 2562
HinP1I GCGC 1 cut(s) 2562
HinfI GANTC 8 cut(s) 177, 869, 1055, 1154, 1834, 2051, 2714, 2868
HpaII CCGG 6 cut(s) 22, 44, 132, 581, 831, 1103
HphI GGTGA 1 cut(s) 1597
Hpy166II GTNNAC 5 cut(s) 1228, 1531, 1619, 2629, 2853
Hpy8I GTNNAC 5 cut(s) 1228, 1531, 1619, 2629, 2853
Hpy99I CGWCG 1 cut(s) 625
HpyAV CCTTC 4 cut(s) 390, 1202, 2642, 2706
HpyCH4III ACNGT 2 cut(s) 1745, 1798
HpyCH4IV ACGT 2 cut(s) 1224, 1502
HpyF10VI GCNNNNNNNGC 7 cut(s) 132, 324, 422, 431, 608, 1424, 2476
HpyF3I CTNAG 2 cut(s) 680, 1487
HpySE526I ACGT 2 cut(s) 1224, 1502
HspAI GCGC 1 cut(s) 2562
Ksp22I TGATCA 3 cut(s) 303, 408, 1888
Kzo9I GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
LmnI GCTCC 3 cut(s) 1334, 1550, 2178
Lsp1109I GCAGC 4 cut(s) 957, 2544, 2849, 2852
LweI GCATC 8 cut(s) 957, 1167, 1279, 1285, 1563, 1747, 2383, 2848
MaeI CTAG 8 cut(s) 507, 701, 705, 855, 974, 1626, 2636, 2877
MaeII ACGT 2 cut(s) 1224, 1502
MaeIII GTNAC 6 cut(s) 997, 1462, 1603, 1906, 1944, 2270
MalI GATC 8 cut(s) 305, 410, 563, 678, 1087, 1890, 2301, 2460
MboI GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
MboII GAAGA 8 cut(s) 27, 749, 1052, 1997, 2214, 2652, 2897, 2912
MflI RGATCY 2 cut(s) 2299, 2458
MhlI GDGCHC 2 cut(s) 1533, 2233
MlyI GAGTC 4 cut(s) 171, 1064, 2723, 2877
MmeI TCCRAC 4 cut(s) 40, 286, 1575, 1610
Mph1103I ATGCAT 4 cut(s) 604, 1372, 1420, 2357
MslI CAYNNNNRTG 6 cut(s) 231, 1004, 1068, 1674, 2024, 2745
MspA1I CMGCKG 2 cut(s) 963, 2843
MspI CCGG 6 cut(s) 22, 44, 132, 581, 831, 1103
MspR9I CCNGG 6 cut(s) 22, 832, 1118, 1159, 2456, 2910
Mva1269I GAATGC 6 cut(s) 604, 907, 1816, 2110, 2152, 2332
MvaI CCWGG 4 cut(s) 1118, 1159, 2456, 2910
MvnI CGCG 1 cut(s) 2562
MwoI GCNNNNNNNGC 7 cut(s) 132, 324, 422, 431, 608, 1424, 2476
NciI CCSGG 2 cut(s) 22, 832
NcoI CCATGG 1 cut(s) 1534
NdeI CATATG 1 cut(s) 2351
NdeII GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
NheI GCTAGC 1 cut(s) 704
NlaIV GGNNCC 4 cut(s) 591, 807, 2190, 2793
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 2 cut(s) 997, 1603
NsiI ATGCAT 4 cut(s) 604, 1372, 1420, 2357
NspI RCATGY 3 cut(s) 1735, 1935, 2816
NspV TTCGAA 1 cut(s) 1776
OliI CACNNNNGTG 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 1935
PaeR7I CTCGAG 1 cut(s) 2711
PceI AGGCCT 2 cut(s) 416, 988
PciI ACATGT 1 cut(s) 2812
PctI GAATGC 6 cut(s) 604, 907, 1816, 2110, 2152, 2332
PfeI GAWTC 4 cut(s) 869, 1154, 1834, 2051
PflFI GACNNNGTC 1 cut(s) 1601
PkrI GCNGC 6 cut(s) 424, 972, 2534, 2561, 2839, 2842
PleI GAGTC 4 cut(s) 171, 1063, 2722, 2876
PpsI GAGTC 4 cut(s) 171, 1063, 2722, 2876
Ppu21I YACGTR 1 cut(s) 1225
PpuMI RGGWCCY 1 cut(s) 216
PscI ACATGT 1 cut(s) 2812
PshBI ATTAAT 2 cut(s) 1514, 2418
Psp5II RGGWCCY 1 cut(s) 216
Psp6I CCWGG 4 cut(s) 1116, 1157, 2454, 2908
PspEI GGTNACC 1 cut(s) 1603
PspFI CCCAGC 2 cut(s) 1760, 2722
PspGI CCWGG 4 cut(s) 1116, 1157, 2454, 2908
PspN4I GGNNCC 4 cut(s) 591, 807, 2190, 2793
PspPI GGNCC 3 cut(s) 216, 2629, 2791
PspPPI RGGWCCY 1 cut(s) 216
PspXI VCTCGAGB 1 cut(s) 2711
PstI CTGCAG 1 cut(s) 2534
PstNI CAGNNNCTG 4 cut(s) 219, 298, 1304, 2843
PsuI RGATCY 2 cut(s) 2299, 2458
PsyI GACNNNGTC 1 cut(s) 1601
PvuII CAGCTG 1 cut(s) 2843
RsaI GTAC 6 cut(s) 697, 827, 1223, 1229, 1736, 2426
RsaNI GTAC 6 cut(s) 696, 826, 1222, 1228, 1735, 2425
RseI CAYNNNNRTG 6 cut(s) 231, 1004, 1068, 1674, 2024, 2745
SatI GCNGC 6 cut(s) 423, 971, 2533, 2560, 2838, 2841
Sau3AI GATC 8 cut(s) 303, 408, 561, 676, 1085, 1888, 2299, 2458
Sau96I GGNCC 3 cut(s) 216, 2629, 2791
ScaI AGTACT 1 cut(s) 697
SchI GAGTC 4 cut(s) 171, 1064, 2723, 2877
ScrFI CCNGG 6 cut(s) 22, 832, 1118, 1159, 2456, 2910
SduI GDGCHC 2 cut(s) 1533, 2233
SfaNI GCATC 8 cut(s) 957, 1167, 1279, 1285, 1563, 1747, 2383, 2848
SfcI CTRYAG 2 cut(s) 873, 2530
Sfr274I CTCGAG 1 cut(s) 2711
SfuI TTCGAA 1 cut(s) 1776
SinI GGWCC 3 cut(s) 216, 2629, 2791
SlaI CTCGAG 1 cut(s) 2711
SmiMI CAYNNNNRTG 6 cut(s) 231, 1004, 1068, 1674, 2024, 2745
SmlI CTYRAG 3 cut(s) 152, 1337, 2711
SmoI CTYRAG 3 cut(s) 152, 1337, 2711
SpeI ACTAGT 1 cut(s) 506
SphI GCATGC 1 cut(s) 1935
SseBI AGGCCT 2 cut(s) 416, 988
SsiI CCGC 3 cut(s) 422, 961, 2560
SspI AATATT 2 cut(s) 2767, 2830
SspMI CTAG 8 cut(s) 507, 701, 705, 855, 974, 1626, 2636, 2877
StuI AGGCCT 2 cut(s) 416, 988
StyD4I CCNGG 6 cut(s) 20, 830, 1116, 1157, 2454, 2908
StyI CCWWGG 3 cut(s) 106, 593, 1534
TaaI ACNGT 2 cut(s) 1745, 1798
TaiI ACGT 2 cut(s) 1227, 1505
TaqI TCGA 7 cut(s) 564, 623, 791, 1010, 1170, 1776, 2712
TaqII GACCGA 1 cut(s) 2672
TatI WGTACW 3 cut(s) 695, 1227, 1734
TauI GCSGC 2 cut(s) 425, 2562
TfiI GAWTC 4 cut(s) 869, 1154, 1834, 2051
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 2 cut(s) 997, 1603
TseI GCWGC 4 cut(s) 970, 2532, 2837, 2840
Tsp45I GTSAC 2 cut(s) 997, 1603
TspGWI ACGGA 2 cut(s) 390, 1563
TspRI CASTG 1 cut(s) 679
Tth111I GACNNNGTC 1 cut(s) 1601
VneI GTGCAC 1 cut(s) 1529
VpaK11BI GGWCC 3 cut(s) 216, 2629, 2791
VspI ATTAAT 2 cut(s) 1514, 2418
XapI RAATTY 8 cut(s) 570, 688, 1030, 1204, 1918, 2212, 2736, 2800
XceI RCATGY 3 cut(s) 1735, 1935, 2816
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XhoI CTCGAG 1 cut(s) 2711
XmiI GTMKAC 1 cut(s) 1618
XspI CTAG 8 cut(s) 507, 701, 705, 855, 974, 1626, 2636, 2877
ZrmI AGTACT 1 cut(s) 697
Zsp2I ATGCAT 4 cut(s) 604, 1372, 1420, 2357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.