pycom02g21650

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013123
Physical Location & Seq
Reverse (-)
19888983 .. 19892128
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21650.1

Sequence Viewer

Length: 1227 bp
ATGGAGTCTCTCTGTCCCCCCGCAGGAGTTATCGCATCCACAAGATTGTCATTCAAACCTCCCAAAACCCAGTCTCAGGCCCCGACTCCCAACCTCCGATTCCCCTTCTCACCAACCCACAACAACCCTTTCAATTTCCCTGCATTTTCCTTCACTTCTTACACTTGTTTCTCCGTTCAAACATCATGCGCTGCTTCAACTTCAGCGACAGCATCGGCTTCCACTTCTCTTCCTTCGTCTTCCGCTTCCAGCGACAACCGCCATTGGGTGGTGCTCATGGAGGCTCCTCCGCAAGGGGCGGCGATTTCCAGACAGCAAGTTATTGAATACTACGTCGAAACCCTTCGAACCGTTTTGGGCAATGAGAAGGATGCTCAAATGTGTATATATGATGCGTCTTGGGATACCCATTTCGGGTTTTGTTGTGATATTGACGAAGAAACTTCCTGCAAGCTCGCACGTTTACCGGGGGTTTTGTTGGTTAAGCCTGACCCGGATTATGTTTCTCAAAAGAAGGATTATAGTCCCCCAAATGTTAGGTCAAGTAATATGTCGTCCTCTAGAAGTGGAAGTGCTTTGTTGTTTCCTCTTTTGAATACAAAAAGGTGGCTTGTTCGAATGGATAAACCCAGCATTGGGGTTGTTACAAAGGCACAAATGGTGGATTATTATGCTCAAATACTAACAAAGGTCCTGGGGAACGAGAAGGATGCACAAATGTGTATTTATCATGTTTCCTGGCAATCTAACTTTGGGTTCTGTTGTGAACTTGATGAAGAGTGTGCACAGGAAGTTGCTGGTGTTCCTGGCGTTTTATCTGTTGAGCCTGACAAGAATTGGGAGTCGGAGAATAAAGATTATGGAGGTAATAATTTACAGAATTCTACCAATCTCTCAACTTCTGGAGCGGCAAGTGAAACAACCCCCACAAAGACAAAGAAACTGTTTGTGACTGGCCTATCGTTTTATACATCTGAGAAAACCCTCCGTGCTGCATTTGAAGGGTATGGAGAGCTTGTTGAAGTTAAAATAATAATGGACAAAATTTCTAAAAGGTCCAAAGGTTATGCATTCATAGAATACACCACGGAGGAAGCTGCTAGCGTAGCTCTCAAAGAGATGAACGGCAAGATCATAAATGGCTGGATGATAGTCGTTGACGTTGCCAAGACTAGCCCTCCAAGATCCAGTAGCAGGGGCCAGACCAGACCAGCAGCAACCAGATGA

Protein Analysis

409

Amino Acids

44.76

Weight (kDa)

6.89

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 86 - 177 7.1e-38 Multiple organellar RNA editing factor 1, MORF domain
MORF_dom PF21864 201 - 291 1.1e-38 Multiple organellar RNA editing factor 1, MORF domain
RRM_1 PF00076 315 - 381 1.1e-18 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20930 AT3G20930
fragaria_vesca FvH4_7g05760 FvH4_7g05760
malus_domestica MD02G1255600.v1.1
prunus_persica Prupe.2G080000_v2.0.a1 Prupe.2G080400_v2.0.a1
pyrus_communis pycom02g21650
rosa_chinensis RchiOBHm_Chr1g0332801
rosa_laevigata RLG00000029643
rosa_multiflora Rmu_ssc0000157.1_g000008
rosa_roxburghii Rroxscaffold_4G00317970
rosa_rugosa Rorug01G0103600
rosa_samantha Rh1AG128400 Rh1BG097600 Rh1CG122200 Rh1DG134000
rosa_wichuraiana Rw1G010500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 268
AccBSI CCGCTC 1 cut(s) 908
AciI CCGC 6 cut(s) 21, 243, 259, 290, 299, 908
AclWI GGATC 1 cut(s) 1179
AcsI RAATTY 2 cut(s) 880, 1044
AcuI CTGAAG 1 cut(s) 186
AfiI CCNNNNNNNGG 9 cut(s) 23, 76, 265, 268, 293, 414, 635, 636, 1194
AgsI TTSAA 8 cut(s) 55, 133, 179, 198, 326, 595, 1001, 1022
AjnI CCWGG 3 cut(s) 693, 737, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AleI CACNNNNGTG 1 cut(s) 718
AluBI AGCT 4 cut(s) 454, 1015, 1097, 1109
AluI AGCT 4 cut(s) 454, 1015, 1097, 1109
Alw21I GWGCWC 2 cut(s) 276, 787
Alw26I GTCTC 2 cut(s) 12, 78
Alw44I GTGCAC 1 cut(s) 783
AlwI GGATC 1 cut(s) 1179
AoxI GGCC 3 cut(s) 78, 955, 1198
ApaLI GTGCAC 1 cut(s) 783
ApeKI GCWGC 4 cut(s) 191, 992, 1097, 1214
ApoI RAATTY 2 cut(s) 880, 1044
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 4 cut(s) 79, 691, 1056, 1198
AsuC2I CCSGG 2 cut(s) 468, 494
AsuHPI GGTGA 1 cut(s) 102
AsuII TTCGAA 2 cut(s) 346, 616
AsuNHI GCTAGC 1 cut(s) 1100
AvaII GGWCC 2 cut(s) 691, 1056
BaeGI GKGCMC 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 276, 787
BbvI GCAGC 3 cut(s) 178, 979, 1084
BceAI ACGGC 1 cut(s) 1141
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 3 cut(s) 695, 739, 807
BciVI GTATCC 1 cut(s) 397
BcnI CCSGG 2 cut(s) 468, 494
BcoDI GTCTC 2 cut(s) 12, 78
BfaI CTAG 3 cut(s) 561, 1101, 1173
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 6 cut(s) 192, 300, 909, 993, 1098, 1215
BlsI GCNGC 6 cut(s) 193, 301, 910, 994, 1099, 1216
Bme1390I CCNGG 5 cut(s) 468, 494, 695, 739, 807
Bme18I GGWCC 2 cut(s) 691, 1056
BmgT120I GGNCC 4 cut(s) 79, 691, 1056, 1198
BmiI GGNNCC 3 cut(s) 81, 285, 1199
BmrFI CCNGG 5 cut(s) 468, 494, 695, 739, 807
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 5 cut(s) 44, 221, 361, 382, 700
BmtI GCTAGC 1 cut(s) 1104
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 924
Bpu14I TTCGAA 2 cut(s) 346, 616
BpuMI CCSGG 2 cut(s) 468, 494
BsaJI CCNNGG 3 cut(s) 467, 694, 1086
BsaXI ACNNNNNCTCC 4 cut(s) 1002, 1032, 1162, 1192
Bsc4I CCNNNNNNNGG 9 cut(s) 23, 76, 265, 268, 293, 414, 635, 636, 1194
Bse1I ACTGG 3 cut(s) 70, 958, 1188
Bse3DI GCAATG 1 cut(s) 367
BseBI CCWGG 3 cut(s) 695, 739, 807
BseDI CCNNGG 3 cut(s) 467, 694, 1086
BseGI GGATG 4 cut(s) 35, 376, 715, 1152
BseLI CCNNNNNNNGG 9 cut(s) 23, 76, 265, 268, 293, 414, 635, 636, 1194
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 2 cut(s) 89, 966
BseNI ACTGG 3 cut(s) 70, 958, 1188
BseRI GAGGAG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 787
BseXI GCAGC 3 cut(s) 178, 979, 1084
BseYI CCCAGC 1 cut(s) 629
BshFI GGCC 3 cut(s) 80, 957, 1200
BsiHKAI GWGCWC 2 cut(s) 276, 787
BsiSI CCGG 2 cut(s) 467, 494
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 9 cut(s) 23, 76, 265, 268, 293, 414, 635, 636, 1194
BsmAI GTCTC 2 cut(s) 12, 78
BsmFI GGGAC 1 cut(s) 510
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 3 cut(s) 80, 957, 1200
Bsp119I TTCGAA 2 cut(s) 346, 616
Bsp1286I GDGCHC 2 cut(s) 276, 787
Bsp143I GATC 2 cut(s) 1131, 1184
BspACI CCGC 6 cut(s) 21, 243, 259, 290, 299, 908
BspANI GGCC 3 cut(s) 80, 957, 1200
BspCNI CTCAG 2 cut(s) 88, 967
BspLI GGNNCC 3 cut(s) 81, 285, 1199
BspOI GCTAGC 1 cut(s) 1104
BspPI GGATC 1 cut(s) 1179
BspT104I TTCGAA 2 cut(s) 346, 616
BsrBI CCGCTC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 3 cut(s) 70, 958, 1188
BssECI CCNNGG 3 cut(s) 467, 694, 1086
BssMI GATC 2 cut(s) 1131, 1184
Bst2UI CCWGG 3 cut(s) 695, 739, 807
Bst4CI ACNGT 2 cut(s) 352, 945
Bst6I CTCTTC 2 cut(s) 234, 771
BstBI TTCGAA 2 cut(s) 346, 616
BstC8I GCNNGC 3 cut(s) 452, 456, 1102
BstDEI CTNAG 2 cut(s) 75, 975
BstDSI CCRYGG 1 cut(s) 1086
BstENI CCTNNNNNAGG 1 cut(s) 291
BstF5I GGATG 4 cut(s) 35, 376, 715, 1152
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 1134, 1187
BstMAI GTCTC 2 cut(s) 12, 78
BstMBI GATC 2 cut(s) 1131, 1184
BstMWI GCNNNNNNNGC 2 cut(s) 258, 1106
BstNI CCWGG 3 cut(s) 695, 739, 807
BstSCI CCNGG 5 cut(s) 466, 492, 693, 737, 805
BstSLI GKGCMC 1 cut(s) 787
BstV1I GCAGC 3 cut(s) 178, 979, 1084
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 1184
BstYI RGATCY 1 cut(s) 1184
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 3 cut(s) 80, 957, 1200
BtgI CCRYGG 1 cut(s) 1086
BtsCI GGATG 4 cut(s) 35, 376, 715, 1152
Cac8I GCNNGC 3 cut(s) 452, 456, 1102
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 4 cut(s) 79, 691, 1056, 1198
CseI GACGC 1 cut(s) 384
CviAII CATG 3 cut(s) 186, 277, 731
DdeI CTNAG 2 cut(s) 75, 975
DpnI GATC 2 cut(s) 1133, 1186
DpnII GATC 2 cut(s) 1131, 1184
Eam1104I CTCTTC 2 cut(s) 234, 771
EarI CTCTTC 2 cut(s) 234, 771
Eco47I GGWCC 2 cut(s) 691, 1056
Eco57I CTGAAG 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 291
EcoO109I RGGNCCY 2 cut(s) 79, 691
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 3 cut(s) 693, 737, 805
EcoT22I ATGCAT 1 cut(s) 1072
FaeI CATG 3 cut(s) 189, 280, 734
FaqI GGGAC 1 cut(s) 510
FatI CATG 3 cut(s) 185, 276, 730
FauI CCCGC 1 cut(s) 28
Fnu4HI GCNGC 6 cut(s) 192, 300, 909, 993, 1098, 1215
FokI GGATG 4 cut(s) 22, 383, 722, 1159
Fsp4HI GCNGC 6 cut(s) 192, 300, 909, 993, 1098, 1215
FspBI CTAG 3 cut(s) 561, 1101, 1173
GlaI GCGC 1 cut(s) 190
GluI GCNGC 6 cut(s) 192, 300, 909, 993, 1098, 1215
GsaI CCCAGC 1 cut(s) 633
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 3 cut(s) 80, 957, 1200
HapII CCGG 2 cut(s) 467, 494
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 191
Hin1II CATG 3 cut(s) 189, 280, 734
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HinfI GANTC 4 cut(s) 5, 85, 99, 842
HpaII CCGG 2 cut(s) 467, 494
HphI GGTGA 1 cut(s) 102
Hpy166II GTNNAC 4 cut(s) 464, 767, 785, 1159
Hpy188I TCNGA 3 cut(s) 98, 847, 976
Hpy188III TCNNGA 3 cut(s) 309, 561, 903
Hpy8I GTNNAC 4 cut(s) 464, 767, 785, 1159
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 8 cut(s) 115, 160, 243, 353, 361, 508, 700, 995
HpyCH4III ACNGT 2 cut(s) 352, 945
HpyCH4IV ACGT 3 cut(s) 333, 460, 1161
HpyCH4V TGCA 6 cut(s) 143, 450, 713, 785, 995, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 1106
HpyF3I CTNAG 2 cut(s) 75, 975
HpySE526I ACGT 3 cut(s) 333, 460, 1161
Hsp92II CATG 3 cut(s) 189, 280, 734
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 2 cut(s) 1131, 1184
LmnI GCTCC 2 cut(s) 289, 905
Lsp1109I GCAGC 3 cut(s) 178, 979, 1084
LweI GCATC 5 cut(s) 44, 221, 361, 382, 700
MaeI CTAG 3 cut(s) 561, 1101, 1173
MaeII ACGT 3 cut(s) 333, 460, 1161
MaeIII GTNAC 2 cut(s) 643, 949
MalI GATC 2 cut(s) 1133, 1186
MbiI CCGCTC 1 cut(s) 908
MboI GATC 2 cut(s) 1131, 1184
MboII GAAGA 4 cut(s) 221, 231, 449, 788
MflI RGATCY 1 cut(s) 1184
MhlI GDGCHC 2 cut(s) 276, 787
MluCI AATT 5 cut(s) 133, 835, 871, 880, 1044
MlyI GAGTC 3 cut(s) 14, 79, 851
MmeI TCCRAC 1 cut(s) 825
Mph1103I ATGCAT 1 cut(s) 1072
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 2 cut(s) 483, 1026
MslI CAYNNNNRTG 1 cut(s) 718
MspI CCGG 2 cut(s) 467, 494
MspR9I CCNGG 5 cut(s) 468, 494, 695, 739, 807
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 3 cut(s) 695, 739, 807
MwoI GCNNNNNNNGC 2 cut(s) 258, 1106
NciI CCSGG 2 cut(s) 468, 494
NdeII GATC 2 cut(s) 1131, 1184
NheI GCTAGC 1 cut(s) 1100
NlaIII CATG 3 cut(s) 189, 280, 734
NlaIV GGNNCC 3 cut(s) 81, 285, 1199
NmuCI GTSAC 1 cut(s) 949
NsiI ATGCAT 1 cut(s) 1072
NspV TTCGAA 2 cut(s) 346, 616
OliI CACNNNNGTG 1 cut(s) 718
PctI GAATGC 1 cut(s) 1070
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 6 cut(s) 193, 301, 910, 994, 1099, 1216
PleI GAGTC 3 cut(s) 13, 79, 850
PpsI GAGTC 3 cut(s) 13, 79, 850
PpuMI RGGWCCY 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 3 cut(s) 693, 737, 805
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 3 cut(s) 693, 737, 805
PspN4I GGNNCC 3 cut(s) 81, 285, 1199
PspPI GGNCC 4 cut(s) 79, 691, 1056, 1198
PspPPI RGGWCCY 1 cut(s) 691
PsuI RGATCY 1 cut(s) 1184
RseI CAYNNNNRTG 1 cut(s) 718
SaqAI TTAA 2 cut(s) 483, 1026
SatI GCNGC 6 cut(s) 192, 300, 909, 993, 1098, 1215
Sau3AI GATC 2 cut(s) 1131, 1184
Sau96I GGNCC 4 cut(s) 79, 691, 1056, 1198
SchI GAGTC 3 cut(s) 14, 79, 851
ScrFI CCNGG 5 cut(s) 468, 494, 695, 739, 807
SduI GDGCHC 2 cut(s) 276, 787
SfaNI GCATC 5 cut(s) 44, 221, 361, 382, 700
SfuI TTCGAA 2 cut(s) 346, 616
SinI GGWCC 2 cut(s) 691, 1056
SmiMI CAYNNNNRTG 1 cut(s) 718
Sse9I AATT 5 cut(s) 133, 835, 871, 880, 1044
SsiI CCGC 6 cut(s) 21, 243, 259, 290, 299, 908
SspMI CTAG 3 cut(s) 561, 1101, 1173
StyD4I CCNGG 5 cut(s) 466, 492, 693, 737, 805
TaaI ACNGT 2 cut(s) 352, 945
TaiI ACGT 3 cut(s) 336, 463, 1164
TaqI TCGA 3 cut(s) 336, 346, 616
TasI AATT 5 cut(s) 133, 835, 871, 880, 1044
TauI GCSGC 2 cut(s) 302, 911
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 2 cut(s) 483, 1026
Tru9I TTAA 2 cut(s) 483, 1026
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 4 cut(s) 191, 992, 1097, 1214
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 3 cut(s) 789, 1063, 1136
TspGWI ACGGA 3 cut(s) 163, 977, 1103
Van91I CCANNNNNTGG 1 cut(s) 268
VneI GTGCAC 1 cut(s) 783
VpaK11BI GGWCC 2 cut(s) 691, 1056
XagI CCTNNNNNAGG 1 cut(s) 291
XapI RAATTY 2 cut(s) 880, 1044
XbaI TCTAGA 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 3 cut(s) 561, 1101, 1173
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.