pycom02g23180

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003610
Physical Location & Seq
Reverse (-)
21318754 .. 21322059
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1191 bp
ATGGAGAGGCGAGGGCGTGGGCGTGGTCGAAATGTTTCTTCTTCCCAATCCACGCGTGGCCGCTCGAACAGGTTCGTTTCGAATTCGACCTCCGCCGCAGCCGCTAAATCCTCGAGCAGAGGCGCAACTGGTACTGAGAAAGCACAGAACGACGCCGTAGATAACCATTCCGACGTTCCCGCCATAGTGGGCACTTGCCCCTTGATGTGCCCGGAAGGAGAGAGGGCGCAGCGTGAGCGGCTTCGTGATTTAGCAGTGTTTGAGAGGCTCCATGGAAATCCCAGACAATCATCTCCGGACCTCGCTGTTAAAAAATTTTTCAGAACTACGTCTAACAAACAAGTTGAAGCATCGGAGGTTCGGCCGCTCCCTGTGTTGGAAGACACCTTAAATTACCTTTTAAACTTGTTTGATTCTAGAGAATATCCTTTTGAAGTGGTTCATGACTTCCTCTTTGATCGAACAAGGTCGATAAGACAGGATCTTAGCATGCAGAATGTTGTCAACGATAAAGTCATCCACATGTATGAGAAAATGGTCAAGTTTCATATCATATCCCTTCGCAAGCTTCGAGGTTGTCGCAGTCAAAATACTTCTTCAACCCATTACCTCAACATGGAACAGCTTGCAAAGACCTTGACATCTCTATTTGATCTGTATGAAGCACATAGGCATTCCAATTCTACATACGAAAACGAAGCTGAGTTTTGTTCGTTTTATGTGCTGCTTCATCTTGGTCCTAACAGCCAACCGATGGGAGAGTCCCTTTCATGGTGGTTTTCTCACTTGCCTTGTTTGTTAATCAAATCGAAGGAAATGTGCTTTTCCCGAAAGATTTTACGATTTTTCCGGATGGGCAACTATAAGCGTTTCTTAAGCACTATAGCAGCTGAGGCATCTTATCTTCAATACTGCATTCTCGAGCCTTATATCAACCAGGTTCGAGTGCTCGCTGTCTCGTGTATAAATAATGGTGGATACAAGCTTCATCCGTATCCGCTGGCACACTTGTCTAAGCTCTTGATGATGACGGAATCAGATTTGGAGTCTCTTTGTAGAGCTTGTGGTCTTGATATTTGCACAGATGAAGAAGGATATAGTTTGTTGCCGACTAAGCAAACCACCTTTTGTCATCCGAAGGATGGATTTCAAAGCTACAGAGTTTTGGGTCTGGAAGAATTCGAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

397

Amino Acids

45.38

Weight (kDa)

8.74

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 69 - 359 2.8e-80 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 3 cut(s) 63, 238, 367
AccII CGCG 1 cut(s) 55
AccIII TCCGGA 2 cut(s) 295, 849
AciI CCGC 8 cut(s) 61, 93, 96, 102, 180, 238, 365, 998
AclWI GGATC 1 cut(s) 489
AcoI YGGCCR 2 cut(s) 58, 362
AcsI RAATTY 3 cut(s) 82, 314, 1178
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 5 cut(s) 376, 616, 754, 771, 1142
AflII CTTAAG 1 cut(s) 874
AflIII ACRYGT 2 cut(s) 53, 522
AgsI TTSAA 5 cut(s) 347, 434, 600, 908, 1151
AjnI CCWGG 1 cut(s) 936
AluBI AGCT 8 cut(s) 568, 625, 701, 890, 985, 1018, 1061, 1155
AluI AGCT 8 cut(s) 568, 625, 701, 890, 985, 1018, 1061, 1155
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 961, 1053
AlwI GGATC 1 cut(s) 489
Ama87I CYCGRG 2 cut(s) 112, 920
Aor13HI TCCGGA 2 cut(s) 295, 849
AoxI GGCC 2 cut(s) 58, 362
ApeKI GCWGC 4 cut(s) 98, 229, 724, 887
ApoI RAATTY 3 cut(s) 82, 314, 1178
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
Asp700I GAANNNNTTC 3 cut(s) 34, 71, 438
AspLEI GCGC 2 cut(s) 125, 229
AspS9I GGNCC 2 cut(s) 298, 737
AsuC2I CCSGG 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 2 cut(s) 112, 920
AvaII GGWCC 2 cut(s) 298, 737
BaeGI GKGCMC 2 cut(s) 194, 212
BauI CACGAG 1 cut(s) 958
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 951
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 4 cut(s) 110, 241, 711, 899
BccI CCATC 3 cut(s) 748, 847, 1136
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 938
BciVI GTATCC 2 cut(s) 971, 1005
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 961, 1053
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 2 cut(s) 882, 1156
BfrI CTTAAG 1 cut(s) 874
BfuI GTATCC 2 cut(s) 971, 1005
BisI GCNGC 9 cut(s) 61, 96, 99, 102, 230, 239, 365, 725, 888
BlsI GCNGC 9 cut(s) 62, 97, 100, 103, 231, 240, 366, 726, 889
Bme1390I CCNGG 2 cut(s) 212, 938
Bme18I GGWCC 2 cut(s) 298, 737
BmeT110I CYCGRG 2 cut(s) 112, 920
BmgT120I GGNCC 2 cut(s) 298, 737
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 2 cut(s) 212, 938
BmsI GCATC 2 cut(s) 359, 905
BpiI GAAGAC 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 891
Bpu14I TTCGAA 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 2 cut(s) 295, 849
Bsc4I CCNNNNNNNGG 5 cut(s) 376, 616, 754, 771, 1142
Bse1I ACTGG 1 cut(s) 133
BseAI TCCGGA 2 cut(s) 295, 849
BseBI CCWGG 1 cut(s) 938
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 5 cut(s) 516, 858, 988, 1132, 1147
BseLI CCNNNNNNNGG 5 cut(s) 376, 616, 754, 771, 1142
BseMII CTCAG 3 cut(s) 126, 693, 882
BseNI ACTGG 1 cut(s) 133
BseSI GKGCMC 2 cut(s) 194, 212
BseX3I CGGCCG 1 cut(s) 362
BseXI GCAGC 4 cut(s) 110, 241, 711, 899
Bsh1236I CGCG 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 2 cut(s) 60, 364
BsiEI CGRYCG 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 951
BsiHKCI CYCGRG 2 cut(s) 112, 920
BsiSI CCGG 3 cut(s) 212, 296, 850
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 5 cut(s) 376, 616, 754, 771, 1142
BsmAI GTCTC 2 cut(s) 961, 1053
BsmFI GGGAC 1 cut(s) 748
BsmI GAATGC 2 cut(s) 673, 915
BsnI GGCC 2 cut(s) 60, 364
BsoBI CYCGRG 2 cut(s) 112, 920
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 194, 212, 951
Bsp13I TCCGGA 2 cut(s) 295, 849
Bsp143I GATC 3 cut(s) 457, 481, 652
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 8 cut(s) 61, 93, 96, 102, 180, 238, 365, 998
BspANI GGCC 2 cut(s) 60, 364
BspCNI CTCAG 3 cut(s) 127, 694, 883
BspEI TCCGGA 2 cut(s) 295, 849
BspFNI CGCG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 269
BspPI GGATC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 874
BsrBI CCGCTC 3 cut(s) 63, 238, 367
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 457, 481, 652
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 958
BssT1I CCWWGG 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 958
Bst2UI CCWGG 1 cut(s) 938
BstACI GRCGYC 1 cut(s) 153
BstAFI CTTAAG 1 cut(s) 874
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 5 cut(s) 491, 566, 627, 951, 1002
BstDEI CTNAG 6 cut(s) 135, 485, 702, 891, 1014, 1113
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 5 cut(s) 516, 858, 988, 1132, 1147
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 2 cut(s) 125, 229
BstKTI GATC 3 cut(s) 460, 484, 655
BstMAI GTCTC 2 cut(s) 961, 1053
BstMBI GATC 3 cut(s) 457, 481, 652
BstMCI CGRYCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 5 cut(s) 101, 235, 238, 893, 1114
BstNI CCWGG 1 cut(s) 938
BstNSI RCATGY 2 cut(s) 493, 526
BstSCI CCNGG 2 cut(s) 210, 936
BstSFI CTRYAG 2 cut(s) 882, 1156
BstSLI GKGCMC 2 cut(s) 194, 212
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 4 cut(s) 110, 241, 711, 899
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 1 cut(s) 362
BsuI GTATCC 2 cut(s) 971, 1005
BsuRI GGCC 2 cut(s) 60, 364
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 5 cut(s) 516, 858, 988, 1132, 1147
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 261
Cac8I GCNNGC 5 cut(s) 491, 566, 627, 951, 1002
CciI TCATGA 1 cut(s) 442
CfoI GCGC 2 cut(s) 125, 229
Cfr13I GGNCC 2 cut(s) 298, 737
CseI GACGC 1 cut(s) 161
CsiI ACCWGGT 1 cut(s) 936
Csp6I GTAC 1 cut(s) 132
CviAII CATG 6 cut(s) 272, 443, 490, 523, 616, 771
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 6 cut(s) 135, 485, 702, 891, 1014, 1113
DpnI GATC 3 cut(s) 459, 483, 654
DpnII GATC 3 cut(s) 457, 481, 652
DraI TTTAAA 1 cut(s) 402
EaeI YGGCCR 2 cut(s) 58, 362
EagI CGGCCG 1 cut(s) 362
EciI GGCGGA 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 298, 737
Eco52I CGGCCG 1 cut(s) 362
Eco88I CYCGRG 2 cut(s) 112, 920
EcoRI GAATTC 2 cut(s) 82, 1178
EcoRII CCWGG 1 cut(s) 936
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 6 cut(s) 275, 446, 493, 526, 619, 774
FalI AAGNNNNNCTT 2 cut(s) 857, 889
FaqI GGGAC 1 cut(s) 748
FatI CATG 6 cut(s) 271, 442, 489, 522, 615, 770
FauI CCCGC 1 cut(s) 187
Fnu4HI GCNGC 9 cut(s) 61, 96, 99, 102, 230, 239, 365, 725, 888
FokI GGATG 5 cut(s) 503, 865, 975, 1119, 1154
Fsp4HI GCNGC 9 cut(s) 61, 96, 99, 102, 230, 239, 365, 725, 888
FspBI CTAG 1 cut(s) 417
GlaI GCGC 2 cut(s) 124, 228
GluI GCNGC 9 cut(s) 61, 96, 99, 102, 230, 239, 365, 725, 888
HaeIII GGCC 2 cut(s) 60, 364
HapII CCGG 3 cut(s) 212, 296, 850
HgaI GACGC 1 cut(s) 161
HhaI GCGC 2 cut(s) 125, 229
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 6 cut(s) 275, 446, 493, 526, 619, 774
Hin6I GCGC 2 cut(s) 123, 227
HinP1I GCGC 2 cut(s) 123, 227
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 2 cut(s) 566, 983
HinfI GANTC 4 cut(s) 413, 761, 1034, 1046
HpaII CCGG 3 cut(s) 212, 296, 850
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 5 cut(s) 172, 323, 355, 1039, 1137
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 2 cut(s) 155, 176
HpyAV CCTTC 5 cut(s) 209, 569, 805, 1085, 1132
HpyCH4IV ACGT 2 cut(s) 174, 329
HpyCH4V TGCA 4 cut(s) 493, 629, 915, 1080
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 235, 238, 893, 1114
HpyF3I CTNAG 6 cut(s) 135, 485, 702, 891, 1014, 1113
HpySE526I ACGT 2 cut(s) 174, 329
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 6 cut(s) 275, 446, 493, 526, 619, 774
HspAI GCGC 2 cut(s) 123, 227
Kpn2I TCCGGA 2 cut(s) 295, 849
Kzo9I GATC 3 cut(s) 457, 481, 652
LmnI GCTCC 2 cut(s) 273, 372
Lsp1109I GCAGC 4 cut(s) 110, 241, 711, 899
LweI GCATC 2 cut(s) 359, 905
MabI ACCWGGT 1 cut(s) 936
MaeI CTAG 1 cut(s) 417
MaeII ACGT 2 cut(s) 174, 329
MalI GATC 3 cut(s) 459, 483, 654
MbiI CCGCTC 3 cut(s) 63, 238, 367
MboI GATC 3 cut(s) 457, 481, 652
MboII GAAGA 7 cut(s) 30, 33, 392, 588, 896, 1100, 1187
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 3 cut(s) 194, 212, 951
MluCI AATT 5 cut(s) 82, 314, 391, 679, 1178
MluI ACGCGT 1 cut(s) 53
MlyI GAGTC 2 cut(s) 770, 1055
MmeI TCCRAC 2 cut(s) 195, 357
MroI TCCGGA 2 cut(s) 295, 849
MroXI GAANNNNTTC 3 cut(s) 34, 71, 438
MseI TTAA 5 cut(s) 309, 389, 401, 800, 875
MslI CAYNNNNRTG 2 cut(s) 521, 525
MspA1I CMGCKG 2 cut(s) 890, 1000
MspCI CTTAAG 1 cut(s) 874
MspI CCGG 3 cut(s) 212, 296, 850
MspR9I CCNGG 2 cut(s) 212, 938
Mva1269I GAATGC 2 cut(s) 673, 915
MvaI CCWGG 1 cut(s) 938
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 5 cut(s) 101, 235, 238, 893, 1114
NciI CCSGG 1 cut(s) 212
NcoI CCATGG 1 cut(s) 271
NdeII GATC 3 cut(s) 457, 481, 652
NlaIII CATG 6 cut(s) 275, 446, 493, 526, 619, 774
NlaIV GGNNCC 1 cut(s) 269
NspI RCATGY 2 cut(s) 493, 526
NspV TTCGAA 1 cut(s) 80
PaeI GCATGC 1 cut(s) 493
PaeR7I CTCGAG 2 cut(s) 112, 920
PagI TCATGA 1 cut(s) 442
PciI ACATGT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 2 cut(s) 673, 915
PdmI GAANNNNTTC 3 cut(s) 34, 71, 438
PfeI GAWTC 2 cut(s) 413, 1034
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 9 cut(s) 62, 97, 100, 103, 231, 240, 366, 726, 889
PleI GAGTC 2 cut(s) 769, 1054
PpsI GAGTC 2 cut(s) 769, 1054
PscI ACATGT 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 936
PspGI CCWGG 1 cut(s) 936
PspN4I GGNNCC 1 cut(s) 269
PspPI GGNCC 2 cut(s) 298, 737
PspXI VCTCGAGB 1 cut(s) 112
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 2 cut(s) 521, 525
SaqAI TTAA 5 cut(s) 309, 389, 401, 800, 875
SatI GCNGC 9 cut(s) 61, 96, 99, 102, 230, 239, 365, 725, 888
Sau3AI GATC 3 cut(s) 457, 481, 652
Sau96I GGNCC 2 cut(s) 298, 737
SchI GAGTC 2 cut(s) 770, 1055
ScrFI CCNGG 2 cut(s) 212, 938
SduI GDGCHC 3 cut(s) 194, 212, 951
SexAI ACCWGGT 1 cut(s) 936
SfaNI GCATC 2 cut(s) 359, 905
SfcI CTRYAG 2 cut(s) 882, 1156
Sfr274I CTCGAG 2 cut(s) 112, 920
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 2 cut(s) 298, 737
SlaI CTCGAG 2 cut(s) 112, 920
SmiMI CAYNNNNRTG 2 cut(s) 521, 525
SmlI CTYRAG 3 cut(s) 112, 874, 920
SmoI CTYRAG 3 cut(s) 112, 874, 920
SphI GCATGC 1 cut(s) 493
Sse9I AATT 5 cut(s) 82, 314, 391, 679, 1178
SsiI CCGC 8 cut(s) 61, 93, 96, 102, 180, 238, 365, 998
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 210, 936
StyI CCWWGG 1 cut(s) 271
TaiI ACGT 2 cut(s) 177, 332
TasI AATT 5 cut(s) 82, 314, 391, 679, 1178
TauI GCSGC 5 cut(s) 63, 98, 104, 241, 367
TfiI GAWTC 2 cut(s) 413, 1034
Tru1I TTAA 5 cut(s) 309, 389, 401, 800, 875
Tru9I TTAA 5 cut(s) 309, 389, 401, 800, 875
TscAI CASTG 1 cut(s) 261
TseI GCWGC 4 cut(s) 98, 229, 724, 887
TspDTI ATGAA 7 cut(s) 431, 536, 675, 719, 759, 977, 1101
TspGWI ACGGA 2 cut(s) 981, 1046
TspRI CASTG 1 cut(s) 261
Van91I CCANNNNNTGG 1 cut(s) 754
Vha464I CTTAAG 1 cut(s) 874
VpaK11BI GGWCC 2 cut(s) 298, 737
XapI RAATTY 3 cut(s) 82, 314, 1178
XbaI TCTAGA 1 cut(s) 416
XceI RCATGY 2 cut(s) 493, 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 2 cut(s) 112, 920
XmnI GAANNNNTTC 3 cut(s) 34, 71, 438
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.