pycom02g25850

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010027
Physical Location & Seq
Forward (+)
23760550 .. 23760819
270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25850.1

Sequence Viewer

Length: 270 bp
ATGGGGATAAAGCTGGTGGAGATTTTGGACCAAGGAGTCAGAATCGTCGCCAGATCTTATTCTCATTGCCCCCAGACCAGCCGCATGTACTACCATCCTCCTCCGCCCGGCCATGAAGACCACCACCACCTTGGGCACCACAATCTTCACAGCGGGGGCGCCACCAGTTCCGGAGGAGGCGATGTTGTGACGGCCCAGATGCGTTGTTTCGGTGCCAAGGCAGCGGGTGCTGGTTCTGATACGAATGAATCTATTCTTTTCTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

90

Amino Acids

9.45

Weight (kDa)

6.74

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AccB1I GGYRCC 3 cut(s) 135, 158, 212
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 4 cut(s) 82, 104, 153, 224
AcoI YGGCCR 1 cut(s) 109
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 107
AluBI AGCT 1 cut(s) 13
AluI AGCT 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 2 cut(s) 109, 192
ApeKI GCWGC 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 252
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 28, 193
AsuC2I CCSGG 1 cut(s) 108
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 138
BanI GGYRCC 3 cut(s) 135, 158, 212
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 102
BceAI ACGGC 1 cut(s) 207
BcnI CCSGG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 162
BglII AGATCT 1 cut(s) 53
BisI GCNGC 2 cut(s) 82, 222
BlsI GCNGC 2 cut(s) 83, 223
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 2 cut(s) 28, 193
BmiI GGNNCC 3 cut(s) 137, 160, 214
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 31, 130, 216
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 64
BseAI TCCGGA 1 cut(s) 170
BseDI CCNNGG 3 cut(s) 31, 130, 216
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMI GCAATG 1 cut(s) 64
BseNI ACTGG 1 cut(s) 165
BseRI GAGGAG 2 cut(s) 90, 189
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 1 cut(s) 233
BshFI GGCC 2 cut(s) 111, 194
BshNI GGYRCC 3 cut(s) 135, 158, 212
BsiSI CCGG 2 cut(s) 108, 171
BslI CCNNNNNNNGG 1 cut(s) 107
BsnI GGCC 2 cut(s) 111, 194
Bsp1286I GDGCHC 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 1 cut(s) 53
BspACI CCGC 4 cut(s) 82, 104, 153, 224
BspANI GGCC 2 cut(s) 111, 194
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 3 cut(s) 137, 160, 214
BspT107I GGYRCC 3 cut(s) 135, 158, 212
BsrDI GCAATG 1 cut(s) 64
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 3 cut(s) 31, 130, 216
BssMI GATC 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 3 cut(s) 31, 130, 216
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 227
BstF5I GGATG 1 cut(s) 94
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 1 cut(s) 56
BstMBI GATC 1 cut(s) 53
BstMWI GCNNNNNNNGC 2 cut(s) 221, 227
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 53
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 53
BsuRI GGCC 2 cut(s) 111, 194
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 94
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 28, 193
Csp6I GTAC 1 cut(s) 88
CviAII CATG 2 cut(s) 85, 113
CviJI RGCY 4 cut(s) 13, 81, 111, 194
CviKI_1 RGCY 4 cut(s) 13, 81, 111, 194
CviQI GTAC 1 cut(s) 88
DinI GGCGCC 1 cut(s) 160
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 1 cut(s) 109
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 3 cut(s) 31, 130, 216
Eco47I GGWCC 1 cut(s) 28
EcoT14I CCWWGG 3 cut(s) 31, 130, 216
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 3 cut(s) 31, 130, 216
FaeI CATG 2 cut(s) 88, 116
FaiI YATR 2 cut(s) 86, 114
FatI CATG 2 cut(s) 84, 112
FauI CCCGC 2 cut(s) 146, 217
Fnu4HI GCNGC 2 cut(s) 82, 222
FokI GGATG 1 cut(s) 81
Fsp4HI GCNGC 2 cut(s) 82, 222
GlaI GCGC 1 cut(s) 160
GluI GCNGC 2 cut(s) 82, 222
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 111, 194
HapII CCGG 2 cut(s) 108, 171
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 2 cut(s) 88, 116
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 3 cut(s) 36, 42, 248
HpaII CCGG 2 cut(s) 108, 171
Hpy188I TCNGA 2 cut(s) 41, 238
Hpy188III TCNNGA 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 227
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 2 cut(s) 88, 116
HspAI GCGC 1 cut(s) 159
KasI GGCGCC 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 170
Kzo9I GATC 1 cut(s) 53
LpnPI CCDG 8 cut(s) 64, 86, 91, 121, 178, 184, 209, 216
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 187
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 2 cut(s) 128, 137
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 1 cut(s) 138
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 1 cut(s) 45
MnlI CCTC 4 cut(s) 108, 111, 167, 170
MroI TCCGGA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 252
MspA1I CMGCKG 2 cut(s) 153, 224
MspI CCGG 2 cut(s) 108, 171
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 221, 227
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 1 cut(s) 108
NdeII GATC 1 cut(s) 53
NlaIII CATG 2 cut(s) 88, 116
NlaIV GGNNCC 3 cut(s) 137, 160, 214
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 2 cut(s) 42, 248
PkrI GCNGC 2 cut(s) 83, 223
PleI GAGTC 1 cut(s) 44
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 137, 160, 214
PspPI GGNCC 2 cut(s) 28, 193
PsuI RGATCY 1 cut(s) 53
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SatI GCNGC 2 cut(s) 82, 222
Sau3AI GATC 1 cut(s) 53
Sau96I GGNCC 2 cut(s) 28, 193
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 138
SetI ASST 2 cut(s) 15, 132
SfaNI GCATC 1 cut(s) 189
SfoI GGCGCC 1 cut(s) 160
SinI GGWCC 1 cut(s) 28
SsiI CCGC 4 cut(s) 82, 104, 153, 224
SspDI GGCGCC 1 cut(s) 158
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 3 cut(s) 31, 130, 216
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 2 cut(s) 42, 248
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 129, 261
VpaK11BI GGWCC 1 cut(s) 28
XceI RCATGY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.