Rorug03G0195500

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001584
Physical Location & Seq
Forward (+)
17192856 .. 17194720
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0195500.1

Sequence Viewer

Length: 651 bp
ATGAGCAAGCTGCAAAGTGAGGCATTGAGGGAGGCCATCTCCCAGATTAAGACTAATTCGGAGACGAAGAAGAGGAAGTTCACCGAGACAGTGGAGTTGCAAATTGGGTTGAAGAACTATGACCCACAAAAGGACAAGCGTTTCAGTGGTTCCGTGAAGCTTCCCCACATTCCTCGTCCTAAGATGAGGGTTTGCATGCTTGGAGATGCTCAGCATGTTGAAGAGGCAGAGAAGATGGGATTGGCCTACATGGATGTGGAATCGCTGAAAAAGCTTAACAAGAACAAGAAGCTGGTCAAGAAGCTTGCAAAGAAGTATCATGCTTTTTTGGCTTCTGAAGCTGTCATCAAGCAAATTCCTCGTCTTTTGGGCCCTGGTCTTAACAAGGCAGGTAAGTTTCCAACTCTGGTGAGTCACCAGGAATCTCTGGAGGGAAAGCTTAATGAAACAAAGGCCATGGTTAAGTTCCAACTCAAGAAGGTTCTTTGTATGGGTGTTGCCGTCGGGAATCTTGCCATGGATGAGAAACAGATATTCCAGAATGTGCAACTCAGTGTCAATTTCTTGGTTTCTCTGTTGAAGAAGAATTGGCAGAATGTGAGATCATTGAACCTGAAAAGCACCATGGGGAAAGCTATCCGAATTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

216

Amino Acids

24.48

Weight (kDa)

10.03

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 21 - 210 3.1e-51 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AcsI RAATTY 2 cut(s) 354, 642
AcuI CTGAAG 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 4 cut(s) 112, 221, 580, 610
AjnI CCWGG 2 cut(s) 373, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 8 cut(s) 10, 160, 274, 292, 304, 341, 439, 635
AluI AGCT 8 cut(s) 10, 160, 274, 292, 304, 341, 439, 635
Alw26I GTCTC 2 cut(s) 56, 80
AoxI GGCC 4 cut(s) 33, 243, 370, 453
ApaI GGGCCC 1 cut(s) 374
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 354, 642
ArsI GACNNNNNNTTYG 2 cut(s) 346, 378
AspS9I GGNCC 2 cut(s) 370, 371
AsuHPI GGTGA 3 cut(s) 73, 407, 421
BaeGI GKGCMC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 374
BccI CCATC 2 cut(s) 44, 229
BceAI ACGGC 1 cut(s) 485
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 2 cut(s) 375, 419
BcoDI GTCTC 2 cut(s) 56, 80
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 1 cut(s) 11
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 2 cut(s) 375, 419
BmgT120I GGNCC 2 cut(s) 370, 371
BmiI GGNNCC 2 cut(s) 151, 372
BmrFI CCNGG 2 cut(s) 375, 419
BmsI GCATC 1 cut(s) 196
BplI GAGNNNNNCTC 2 cut(s) 23, 55
BpmI CTGGAG 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 458
BsaJI CCNNGG 4 cut(s) 373, 456, 516, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 130
BseBI CCWGG 2 cut(s) 375, 419
BseDI CCNNGG 4 cut(s) 373, 456, 516, 624
BseGI GGATG 2 cut(s) 259, 526
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMII CTCAG 2 cut(s) 224, 565
BseSI GKGCMC 1 cut(s) 374
BshFI GGCC 4 cut(s) 35, 245, 372, 455
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 2 cut(s) 56, 80
BsmBI CGTCTC 1 cut(s) 56
BsnI GGCC 4 cut(s) 35, 245, 372, 455
Bsp120I GGGCCC 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 374
Bsp143I GATC 1 cut(s) 602
Bsp1720I GCTNAGC 1 cut(s) 210
Bsp19I CCATGG 3 cut(s) 456, 516, 624
BspANI GGCC 4 cut(s) 35, 245, 372, 455
BspCNI CTCAG 2 cut(s) 223, 564
BspLI GGNNCC 2 cut(s) 151, 372
BspMI ACCTGC 1 cut(s) 380
BssECI CCNNGG 4 cut(s) 373, 456, 516, 624
BssMI GATC 1 cut(s) 602
BssT1I CCWWGG 3 cut(s) 456, 516, 624
Bst2UI CCWGG 2 cut(s) 375, 419
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 2 cut(s) 65, 216
BstC8I GCNNGC 3 cut(s) 8, 197, 306
BstDEI CTNAG 3 cut(s) 180, 210, 551
BstDSI CCRYGG 3 cut(s) 456, 516, 624
BstF5I GGATG 2 cut(s) 259, 526
BstKTI GATC 1 cut(s) 605
BstMAI GTCTC 2 cut(s) 56, 80
BstMBI GATC 1 cut(s) 602
BstMWI GCNNNNNNNGC 3 cut(s) 271, 329, 338
BstNI CCWGG 2 cut(s) 375, 419
BstNSI RCATGY 2 cut(s) 199, 218
BstSCI CCNGG 2 cut(s) 373, 417
BstSLI GKGCMC 1 cut(s) 374
BsuRI GGCC 4 cut(s) 35, 245, 372, 455
BtgI CCRYGG 3 cut(s) 456, 516, 624
BtsCI GGATG 2 cut(s) 259, 526
BtsIMutI CAGTG 3 cut(s) 96, 151, 559
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 3 cut(s) 8, 197, 306
Cfr13I GGNCC 2 cut(s) 370, 371
CviAII CATG 7 cut(s) 196, 215, 250, 320, 457, 517, 625
DdeI CTNAG 3 cut(s) 180, 210, 551
DpnI GATC 1 cut(s) 604
DpnII GATC 1 cut(s) 602
Eam1104I CTCTTC 2 cut(s) 65, 216
EarI CTCTTC 2 cut(s) 65, 216
Eco130I CCWWGG 3 cut(s) 456, 516, 624
Eco24I GRGCYC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 373, 417
EcoT14I CCWWGG 3 cut(s) 456, 516, 624
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 3 cut(s) 456, 516, 624
Esp3I CGTCTC 1 cut(s) 56
FaeI CATG 7 cut(s) 199, 218, 253, 323, 460, 520, 628
FaiI YATR 9 cut(s) 120, 197, 216, 251, 321, 458, 491, 518, 626
FatI CATG 7 cut(s) 195, 214, 249, 319, 456, 516, 624
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 2 cut(s) 266, 533
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 1 cut(s) 11
GluI GCNGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 4 cut(s) 35, 245, 372, 455
Hin1II CATG 7 cut(s) 199, 218, 253, 323, 460, 520, 628
HindIII AAGCTT 4 cut(s) 158, 272, 302, 437
HinfI GANTC 4 cut(s) 260, 412, 422, 508
HphI GGTGA 3 cut(s) 73, 407, 421
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 3 cut(s) 61, 337, 641
Hpy188III TCNNGA 5 cut(s) 298, 428, 475, 505, 538
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 13, 100, 195, 308, 547
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 329, 338
HpyF3I CTNAG 3 cut(s) 180, 210, 551
Hsp92II CATG 7 cut(s) 199, 218, 253, 323, 460, 520, 628
Kzo9I GATC 1 cut(s) 602
LweI GCATC 1 cut(s) 196
MaeIII GTNAC 1 cut(s) 413
MalI GATC 1 cut(s) 604
MboI GATC 1 cut(s) 602
MboII GAAGA 7 cut(s) 79, 82, 124, 233, 244, 592, 595
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 6 cut(s) 55, 102, 354, 559, 586, 642
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 2 cut(s) 425, 493
MnlI CCTC 9 cut(s) 13, 21, 25, 66, 180, 183, 217, 369, 424
MseI TTAA 5 cut(s) 48, 276, 381, 441, 462
MslI CAYNNNNRTG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 375, 419
MvaI CCWGG 2 cut(s) 375, 419
MwoI GCNNNNNNNGC 3 cut(s) 271, 329, 338
NcoI CCATGG 3 cut(s) 456, 516, 624
NdeII GATC 1 cut(s) 602
NlaIII CATG 7 cut(s) 199, 218, 253, 323, 460, 520, 628
NlaIV GGNNCC 2 cut(s) 151, 372
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 2 cut(s) 199, 218
PaeI GCATGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 260, 422, 508
PkrI GCNGC 1 cut(s) 12
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
Psp6I CCWGG 2 cut(s) 373, 417
PspGI CCWGG 2 cut(s) 373, 417
PspN4I GGNNCC 2 cut(s) 151, 372
PspOMI GGGCCC 1 cut(s) 370
PspPI GGNCC 2 cut(s) 370, 371
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 5 cut(s) 48, 276, 381, 441, 462
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 1 cut(s) 602
Sau96I GGNCC 2 cut(s) 370, 371
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 2 cut(s) 375, 419
SduI GDGCHC 1 cut(s) 374
SfaNI GCATC 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SphI GCATGC 1 cut(s) 199
Sse9I AATT 6 cut(s) 55, 102, 354, 559, 586, 642
StyD4I CCNGG 2 cut(s) 373, 417
StyI CCWWGG 3 cut(s) 456, 516, 624
TaaI ACNGT 1 cut(s) 91
TasI AATT 6 cut(s) 55, 102, 354, 559, 586, 642
TfiI GAWTC 3 cut(s) 260, 422, 508
Tru1I TTAA 5 cut(s) 48, 276, 381, 441, 462
Tru9I TTAA 5 cut(s) 48, 276, 381, 441, 462
TscAI CASTG 3 cut(s) 96, 151, 559
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 459
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 3 cut(s) 96, 151, 559
XapI RAATTY 2 cut(s) 354, 642
XceI RCATGY 2 cut(s) 199, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.