Rh3CG277500

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001584
Physical Location & Seq
Forward (+)
27803543 .. 27817202
13660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG277500.1

Sequence Viewer

Length: 2904 bp
ATGGCTGGGGCGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTAACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCGTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCATTGGAGGACAGCTACTTGAAGTACATCATCGAGCATGGAGGAAGAGGAAATGCTTTCACTTCTGAAGAACACACCAGCTATCGTTTCGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGCGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAACTCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGAATACTTTGTGGGTTCGACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTAAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCTTGCATGTATCTTGGTCACCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGCGGGCTGGTGAATCATATTCTACTTTGGACTTTTCTTTCTTTGAAGTAGATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACGTTTCCCTGTTACAGCAGTCCGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTATTTGTGAGACAAAGTTTCATTATGAGGACAAAATTGAGCCTCTTAATTACGTGATCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATTGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAAAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGTGAATTTACACCTACCAGCACGTAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAAAGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCAGCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTCATTTGTTAATGGATTACTTGAATGATTATGCGTATTATGCTGAGGTTGCTGGACTGTACTGTGGAATATACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAACGGTCACAAAGGAGTACCAAAATTACAAATTTGAACAGTCCTATGAGCAGGCTATTTACTATTGCTCATTAGTTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTACTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCATAGTGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATGTTTTTTTTAAGGGTTCAAACCGTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGGAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTATTTGTTCTTATTGCAAAGCAACCAGCCTTTCACCAACTTAGAATAGTTGAGCAACTTGGTTACATAACTCGTCTTTCACAGAGGAATGATTGTGGTATCCGTGGACTGCAGTTCATTATACAATCCACAGTGAAGGGTCCAGGACGTATTGATTTGAGAGTGGAGGAATTTCTCAAGACGTTTGAGAGTAAGCTTTATGAGATGACAAATAACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACCAGAACTTGAGGGAAGAAGCTGAATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTTCAATGAGCATATAAAAGTTGGGGCACCTCATAAGAGGACATTGAGTGTACGAGTATATGGGAATCGTCATTCATTTGAGTATGCATCAGATAAAAGATCCCTAGTGCAACCTTGCACCGTCAAAATCGATGATATTTTCGCTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAGCTCCGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

967

Amino Acids

111.7

Weight (kDa)

6.36

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 1.3e-33 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 8.8e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 657 2.5e-101 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 662 - 848 1.2e-10 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 773 - 868 1.1e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1767
AccB1I GGYRCC 1 cut(s) 2722
AciI CCGC 1 cut(s) 940
AclWI GGATC 5 cut(s) 68, 95, 901, 2083, 2790
AcoI YGGCCR 2 cut(s) 877, 1949
AcuI CTGAAG 5 cut(s) 312, 458, 1222, 2094, 2285
AfiI CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2216, 2864
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AhlI ACTAGT 1 cut(s) 1643
AjnI CCWGG 3 cut(s) 1343, 2262, 2446
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw26I GTCTC 2 cut(s) 1112, 1473
AlwI GGATC 5 cut(s) 68, 95, 901, 2083, 2790
AoxI GGCC 9 cut(s) 175, 667, 812, 848, 877, 1009, 1949, 2031, 2218
ApeKI GCWGC 4 cut(s) 122, 491, 2657, 2668
AseI ATTAAT 1 cut(s) 321
AspS9I GGNCC 6 cut(s) 175, 848, 1939, 2031, 2444, 2622
AsuC2I CCSGG 1 cut(s) 1625
AsuHPI GGTGA 8 cut(s) 743, 824, 860, 959, 991, 1334, 1762, 2330
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1939, 2444, 2622
BaeGI GKGCMC 1 cut(s) 2725
BalI TGGCCA 1 cut(s) 1951
BanI GGYRCC 1 cut(s) 2722
BanII GRGCYC 2 cut(s) 1344, 1385
BbvCI CCTCAGC 1 cut(s) 1698
BbvI GCAGC 4 cut(s) 109, 503, 2669, 2680
BccI CCATC 7 cut(s) 158, 323, 446, 918, 1951, 2158, 2612
BciT130I CCWGG 3 cut(s) 1345, 2264, 2448
BciVI GTATCC 2 cut(s) 562, 2414
BclI TGATCA 2 cut(s) 1164, 1848
BcnI CCSGG 1 cut(s) 1625
BcoDI GTCTC 2 cut(s) 1112, 1473
BcuI ACTAGT 1 cut(s) 1643
BfaI CTAG 4 cut(s) 213, 1644, 1967, 2801
BfmI CTRYAG 3 cut(s) 396, 1934, 2414
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 2 cut(s) 562, 2414
BglII AGATCT 1 cut(s) 1996
BisI GCNGC 4 cut(s) 123, 492, 2658, 2669
BlsI GCNGC 4 cut(s) 124, 493, 2659, 2670
BmcAI AGTACT 1 cut(s) 2071
Bme1390I CCNGG 4 cut(s) 1345, 1625, 2264, 2448
Bme18I GGWCC 3 cut(s) 1939, 2444, 2622
BmgT120I GGNCC 6 cut(s) 175, 848, 1939, 2031, 2444, 2622
BmiI GGNNCC 6 cut(s) 1384, 1621, 2445, 2623, 2624, 2724
BmrFI CCNGG 4 cut(s) 1345, 1625, 2264, 2448
BmsI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2010, 2792
BpmI CTGGAG 4 cut(s) 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1698
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 6 cut(s) 212, 2011, 2465, 2582, 2599, 2612
BpuMI CCSGG 1 cut(s) 1625
Bsa29I ATCGAT 2 cut(s) 2550, 2826
BsaAI YACGTR 3 cut(s) 1162, 1442, 2098
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 5 cut(s) 129, 1343, 1458, 1623, 2407
BsaWI WCCGGW 2 cut(s) 1073, 2885
Bsc4I CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2216, 2864
Bse1I ACTGG 4 cut(s) 733, 1211, 1508, 1794
Bse3DI GCAATG 2 cut(s) 1223, 2539
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 3 cut(s) 1345, 2264, 2448
BseCI ATCGAT 2 cut(s) 2550, 2826
BseDI CCNNGG 5 cut(s) 129, 1343, 1458, 1623, 2407
BseGI GGATG 5 cut(s) 139, 499, 929, 1452, 2150
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2216, 2864
BseMI GCAATG 2 cut(s) 1223, 2539
BseMII CTCAG 4 cut(s) 374, 1689, 2070, 2654
BseNI ACTGG 4 cut(s) 733, 1211, 1508, 1794
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2725
BseXI GCAGC 4 cut(s) 109, 503, 2669, 2680
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 2 cut(s) 510, 2078
BshFI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1951, 2033, 2220
BshNI GGYRCC 1 cut(s) 2722
BshVI ATCGAT 2 cut(s) 2550, 2826
BsiSI CCGG 3 cut(s) 1074, 1625, 2886
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1590, 2635
BslI CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2216, 2864
BsmAI GTCTC 2 cut(s) 1112, 1473
BsmFI GGGAC 3 cut(s) 761, 1590, 2635
BsnI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1951, 2033, 2220
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 1344, 1385, 2725
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 940
BspANI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1951, 2033, 2220
BspCNI CTCAG 4 cut(s) 373, 1690, 2071, 2655
BspDI ATCGAT 2 cut(s) 2550, 2826
BspLI GGNNCC 6 cut(s) 1384, 1621, 2445, 2623, 2624, 2724
BspMAI CTGCAG 1 cut(s) 2418
BspPI GGATC 5 cut(s) 68, 95, 901, 2083, 2790
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2722
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 2 cut(s) 1223, 2539
BsrI ACTGG 4 cut(s) 733, 1211, 1508, 1794
BssECI CCNNGG 5 cut(s) 129, 1343, 1458, 1623, 2407
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 3 cut(s) 1345, 2264, 2448
Bst4CI ACNGT 9 cut(s) 775, 1713, 1718, 1798, 1861, 1896, 2129, 2437, 2818
Bst6I CTCTTC 3 cut(s) 12, 265, 333
BstAFI CTTAAG 1 cut(s) 1472
BstAPI GCANNNNNTGC 1 cut(s) 2654
BstBAI YACGTR 3 cut(s) 1162, 1442, 2098
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 5 cut(s) 942, 1009, 1040, 1816, 1908
BstDEI CTNAG 7 cut(s) 360, 485, 1698, 2004, 2079, 2345, 2663
BstDSI CCRYGG 3 cut(s) 129, 1458, 2407
BstEII GGTNACC 1 cut(s) 866
BstENI CCTNNNNNAGG 2 cut(s) 674, 2214
BstF5I GGATG 5 cut(s) 139, 499, 929, 1452, 2150
BstMAI GTCTC 2 cut(s) 1112, 1473
BstMWI GCNNNNNNNGC 7 cut(s) 174, 478, 1270, 1694, 1703, 2566, 2654
BstNI CCWGG 3 cut(s) 1345, 2264, 2448
BstNSI RCATGY 3 cut(s) 859, 1024, 1336
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 4 cut(s) 1343, 1623, 2262, 2446
BstSFI CTRYAG 3 cut(s) 396, 1934, 2414
BstSLI GKGCMC 1 cut(s) 2725
BstV1I GCAGC 4 cut(s) 109, 503, 2669, 2680
BstX2I RGATCY 3 cut(s) 893, 1996, 2795
BstXI CCANNNNNNTGG 1 cut(s) 1616
BstYI RGATCY 3 cut(s) 893, 1996, 2795
Bsu15I ATCGAT 2 cut(s) 2550, 2826
BsuI GTATCC 2 cut(s) 562, 2414
BsuRI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1951, 2033, 2220
BsuTUI ATCGAT 2 cut(s) 2550, 2826
BtgI CCRYGG 3 cut(s) 129, 1458, 2407
BtsCI GGATG 5 cut(s) 139, 499, 929, 1452, 2150
BtsIMutI CAGTG 3 cut(s) 145, 2442, 2660
Cac8I GCNNGC 5 cut(s) 942, 1009, 1040, 1816, 1908
Cfr13I GGNCC 6 cut(s) 175, 848, 1939, 2031, 2444, 2622
ClaI ATCGAT 2 cut(s) 2550, 2826
DdeI CTNAG 7 cut(s) 360, 485, 1698, 2004, 2079, 2345, 2663
DraI TTTAAA 1 cut(s) 1822
EaeI YGGCCR 2 cut(s) 877, 1949
Eam1104I CTCTTC 3 cut(s) 12, 265, 333
EarI CTCTTC 3 cut(s) 12, 265, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 669, 2220
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco47I GGWCC 3 cut(s) 1939, 2444, 2622
Eco57I CTGAAG 5 cut(s) 312, 458, 1222, 2094, 2285
Eco91I GGTNACC 1 cut(s) 866
EcoNI CCTNNNNNAGG 2 cut(s) 674, 2214
EcoO109I RGGNCCY 4 cut(s) 175, 848, 2031, 2622
EcoO65I GGTNACC 1 cut(s) 866
EcoRI GAATTC 3 cut(s) 1389, 2239, 2522
EcoRII CCWGG 3 cut(s) 1343, 2262, 2446
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 627, 2785
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 3 cut(s) 761, 1590, 2635
FauI CCCGC 1 cut(s) 933
FauNDI CATATG 2 cut(s) 198, 1531
FbaI TGATCA 2 cut(s) 1164, 1848
Fnu4HI GCNGC 4 cut(s) 123, 492, 2658, 2669
FokI GGATG 5 cut(s) 146, 486, 936, 1439, 2137
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 4 cut(s) 123, 492, 2658, 2669
FspBI CTAG 4 cut(s) 213, 1644, 1967, 2801
GluI GCNGC 4 cut(s) 123, 492, 2658, 2669
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 4 cut(s) 478, 776, 807, 1369
HaeIII GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1951, 2033, 2220
HapII CCGG 3 cut(s) 1074, 1625, 2886
HindIII AAGCTT 5 cut(s) 1271, 1816, 2180, 2498, 2558
HinfI GANTC 8 cut(s) 496, 760, 950, 1293, 1304, 2081, 2172, 2761
HpaII CCGG 3 cut(s) 1074, 1625, 2886
HphI GGTGA 8 cut(s) 743, 824, 860, 959, 991, 1334, 1762, 2330
Hpy166II GTNNAC 3 cut(s) 2254, 2411, 2747
Hpy8I GTNNAC 3 cut(s) 2254, 2411, 2747
HpyCH4III ACNGT 9 cut(s) 775, 1713, 1718, 1798, 1861, 1896, 2129, 2437, 2818
HpyCH4IV ACGT 7 cut(s) 1053, 1161, 1441, 1584, 2097, 2452, 2486
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 478, 1270, 1694, 1703, 2566, 2654
HpyF3I CTNAG 7 cut(s) 360, 485, 1698, 2004, 2079, 2345, 2663
HpySE526I ACGT 7 cut(s) 1053, 1161, 1441, 1584, 2097, 2452, 2486
KflI GGGWCCC 1 cut(s) 2622
Ksp22I TGATCA 2 cut(s) 1164, 1848
LmnI GCTCC 3 cut(s) 1388, 1625, 2888
Lsp1109I GCAGC 4 cut(s) 109, 503, 2669, 2680
LweI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2010, 2792
MaeI CTAG 4 cut(s) 213, 1644, 1967, 2801
MaeII ACGT 7 cut(s) 1053, 1161, 1441, 1584, 2097, 2452, 2486
MaeIII GTNAC 7 cut(s) 866, 1062, 1750, 1861, 2045, 2366, 2850
MfeI CAATTG 2 cut(s) 526, 1600
MflI RGATCY 3 cut(s) 893, 1996, 2795
MhlI GDGCHC 3 cut(s) 1344, 1385, 2725
MlsI TGGCCA 1 cut(s) 1951
MluNI TGGCCA 1 cut(s) 1951
MlyI GAGTC 3 cut(s) 1313, 2090, 2181
Mox20I TGGCCA 1 cut(s) 1951
Mph1103I ATGCAT 2 cut(s) 627, 2785
MscI TGGCCA 1 cut(s) 1951
MslI CAYNNNNRTG 4 cut(s) 486, 922, 1614, 2274
Msp20I TGGCCA 1 cut(s) 1951
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1472
MspI CCGG 3 cut(s) 1074, 1625, 2886
MspR9I CCNGG 4 cut(s) 1345, 1625, 2264, 2448
MunI CAATTG 2 cut(s) 526, 1600
MvaI CCWGG 3 cut(s) 1345, 2264, 2448
MwoI GCNNNNNNNGC 7 cut(s) 174, 478, 1270, 1694, 1703, 2566, 2654
NciI CCSGG 1 cut(s) 1625
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 198, 1531
NlaIV GGNNCC 6 cut(s) 1384, 1621, 2445, 2623, 2624, 2724
NmuCI GTSAC 4 cut(s) 866, 1750, 1861, 2850
NsiI ATGCAT 2 cut(s) 627, 2785
NspI RCATGY 3 cut(s) 859, 1024, 1336
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 2 cut(s) 669, 2220
PciI ACATGT 1 cut(s) 1332
PfeI GAWTC 5 cut(s) 496, 760, 950, 1293, 2761
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2446
PkrI GCNGC 4 cut(s) 124, 493, 2659, 2670
PleI GAGTC 3 cut(s) 1312, 2089, 2180
PpsI GAGTC 3 cut(s) 1312, 2089, 2180
Ppu21I YACGTR 3 cut(s) 1162, 1442, 2098
PpuMI RGGWCCY 1 cut(s) 2622
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 1 cut(s) 321
PsiI TTATAA 1 cut(s) 1767
Psp5II RGGWCCY 1 cut(s) 2622
Psp6I CCWGG 3 cut(s) 1343, 2262, 2446
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 1343, 2262, 2446
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1384, 1621, 2445, 2623, 2624, 2724
PspPI GGNCC 6 cut(s) 175, 848, 1939, 2031, 2444, 2622
PspPPI RGGWCCY 1 cut(s) 2622
PstI CTGCAG 1 cut(s) 2418
PsuI RGATCY 3 cut(s) 893, 1996, 2795
PvuII CAGCTG 1 cut(s) 389
RseI CAYNNNNRTG 4 cut(s) 486, 922, 1614, 2274
SatI GCNGC 4 cut(s) 123, 492, 2658, 2669
Sau96I GGNCC 6 cut(s) 175, 848, 1939, 2031, 2444, 2622
ScaI AGTACT 1 cut(s) 2071
SchI GAGTC 3 cut(s) 1313, 2090, 2181
ScrFI CCNGG 4 cut(s) 1345, 1625, 2264, 2448
SduI GDGCHC 3 cut(s) 1344, 1385, 2725
SfaNI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2010, 2792
SfcI CTRYAG 3 cut(s) 396, 1934, 2414
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 3 cut(s) 1939, 2444, 2622
SmiMI CAYNNNNRTG 4 cut(s) 486, 922, 1614, 2274
SmlI CTYRAG 7 cut(s) 191, 1472, 2026, 2480, 2561, 2578, 2627
SmoI CTYRAG 7 cut(s) 191, 1472, 2026, 2480, 2561, 2578, 2627
SpeI ACTAGT 1 cut(s) 1643
SseBI AGGCCT 2 cut(s) 669, 2220
SsiI CCGC 1 cut(s) 940
SspI AATATT 2 cut(s) 703, 1171
SspMI CTAG 4 cut(s) 213, 1644, 1967, 2801
StuI AGGCCT 2 cut(s) 669, 2220
StyD4I CCNGG 4 cut(s) 1343, 1623, 2262, 2446
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 9 cut(s) 775, 1713, 1718, 1798, 1861, 1896, 2129, 2437, 2818
TaiI ACGT 7 cut(s) 1056, 1164, 1444, 1587, 2100, 2455, 2489
TaqI TCGA 7 cut(s) 258, 315, 547, 697, 2272, 2550, 2826
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 5 cut(s) 51, 249, 518, 1713, 2069
TfiI GAWTC 5 cut(s) 496, 760, 950, 1293, 2761
TscAI CASTG 3 cut(s) 145, 2442, 2667
TseFI GTSAC 4 cut(s) 866, 1750, 1861, 2850
TseI GCWGC 4 cut(s) 122, 491, 2657, 2668
Tsp45I GTSAC 4 cut(s) 866, 1750, 1861, 2850
TspGWI ACGGA 4 cut(s) 53, 654, 1475, 2396
TspRI CASTG 3 cut(s) 145, 2442, 2667
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1939, 2444, 2622
VspI ATTAAT 1 cut(s) 321
XagI CCTNNNNNAGG 2 cut(s) 674, 2214
XceI RCATGY 3 cut(s) 859, 1024, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1736
XspI CTAG 4 cut(s) 213, 1644, 1967, 2801
ZrmI AGTACT 1 cut(s) 2071
Zsp2I ATGCAT 2 cut(s) 627, 2785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.