RchiOBHm_Chr3g0482151

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001584
Physical Location & Seq
Forward (+)
28225111 .. 28238526
13416 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44705

Sequence Viewer

Length: 2931 bp
ATGGCTGTGGGGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTCAAGTCCTTCTCATCAGCGATCCTGATACTGACAAGGCAACTGCTCTTTCATCTCAGACTTGTTTCTCTTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATAGTTACTCGAAGTACATCACTGAGCATGGAGGAAGAACAAATGCTTTCACAGCTGCAGAACACACCAACTTTTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCTGATGGCTGGAGGATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTTGTACAGGCAATTGGGATACTTTGGAGGTTCGACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAATGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTATCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCATGAGCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGCGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATGCAAACATGTTCGAGCCCTGGTATGAAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATAAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCAACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATACAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGGAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTCAGGTTGCTGAACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTCTTGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGGAGTACCAAAATTACAAATTTCAACAGCCTTATGAGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCAATGATGCTCTCAAGGGCCTTCGTAGACTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATATTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAGGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATGAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTGCTTGTTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACGTAACTGCTCTTTTACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

976

Amino Acids

112.46

Weight (kDa)

6.0

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 47 - 174 4.2e-34 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 202 - 379 1e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 387 - 666 1.6e-102 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 671 - 857 1.4e-10 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 779 - 877 8.7e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1794, 2727
AccB1I GGYRCC 1 cut(s) 2749
AccI GTMKAC 1 cut(s) 2067
AccII CGCG 1 cut(s) 2436
AciI CCGC 2 cut(s) 2434, 2436
AclI AACGTT 1 cut(s) 2513
AclWI GGATC 4 cut(s) 68, 95, 934, 2110
AcoI YGGCCR 2 cut(s) 910, 1976
AcuI CTGAAG 5 cut(s) 491, 1255, 1674, 2121, 2312
AfiI CCNNNNNNNGG 5 cut(s) 265, 709, 1658, 2243, 2891
AflII CTTAAG 1 cut(s) 1505
AflIII ACRYGT 1 cut(s) 1365
AjnI CCWGG 4 cut(s) 201, 1376, 2289, 2473
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 3 cut(s) 829, 1145, 1506
AlwI GGATC 4 cut(s) 68, 95, 934, 2110
ApeKI GCWGC 6 cut(s) 155, 323, 524, 2332, 2684, 2695
AseI ATTAAT 3 cut(s) 354, 1188, 1955
Asp700I GAANNNNTTC 1 cut(s) 2001
AspS9I GGNCC 6 cut(s) 208, 881, 1966, 2058, 2471, 2649
AsuHPI GGTGA 7 cut(s) 776, 857, 992, 1024, 1367, 1545, 1789
AsuII TTCGAA 1 cut(s) 730
AvaII GGWCC 3 cut(s) 1966, 2471, 2649
BaeGI GKGCMC 1 cut(s) 2752
BalI TGGCCA 1 cut(s) 1978
BanI GGYRCC 1 cut(s) 2749
BanII GRGCYC 2 cut(s) 1377, 1418
Bbv12I GWGCWC 1 cut(s) 1053
BbvI GCAGC 6 cut(s) 142, 310, 536, 2319, 2696, 2707
BccI CCATC 7 cut(s) 191, 356, 479, 951, 1978, 2185, 2639
BciT130I CCWGG 4 cut(s) 203, 1378, 2291, 2475
BciVI GTATCC 3 cut(s) 559, 595, 2441
BclI TGATCA 1 cut(s) 1875
BcoDI GTCTC 3 cut(s) 829, 1145, 1506
BfaI CTAG 2 cut(s) 246, 1994
BfmI CTRYAG 3 cut(s) 324, 1961, 2441
BfrI CTTAAG 1 cut(s) 1505
BfuI GTATCC 3 cut(s) 559, 595, 2441
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 2023
BisI GCNGC 6 cut(s) 156, 324, 525, 2333, 2685, 2696
BlsI GCNGC 6 cut(s) 157, 325, 526, 2334, 2686, 2697
BmcAI AGTACT 1 cut(s) 2098
Bme1390I CCNGG 4 cut(s) 203, 1378, 2291, 2475
Bme18I GGWCC 3 cut(s) 1966, 2471, 2649
BmgT120I GGNCC 6 cut(s) 208, 881, 1966, 2058, 2471, 2649
BmiI GGNNCC 6 cut(s) 1417, 1648, 2472, 2650, 2651, 2751
BmrFI CCNGG 4 cut(s) 203, 1378, 2291, 2475
BmrI ACTGGG 1 cut(s) 2822
BmsI GCATC 8 cut(s) 415, 523, 667, 1027, 1348, 1448, 2037, 2819
BmuI ACTGGG 1 cut(s) 2822
BpmI CTGGAG 5 cut(s) 224, 511, 809, 840, 1402
Bpu10I CCTNAGC 1 cut(s) 1725
Bpu14I TTCGAA 1 cut(s) 730
BpuEI CTTGAG 5 cut(s) 245, 2038, 2609, 2626, 2639
Bsa29I ATCGAT 2 cut(s) 2577, 2853
BsaAI YACGTR 4 cut(s) 1195, 1475, 2125, 2398
BsaBI GATNNNNATC 1 cut(s) 1026
BsaJI CCNNGG 3 cut(s) 162, 1376, 2434
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 709, 1658, 2243, 2891
Bse1I ACTGG 5 cut(s) 766, 1244, 1535, 1821, 2828
Bse3DI GCAATG 2 cut(s) 1256, 2566
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 4 cut(s) 203, 1378, 2291, 2475
BseCI ATCGAT 2 cut(s) 2577, 2853
BseDI CCNNGG 3 cut(s) 162, 1376, 2434
BseGI GGATG 6 cut(s) 172, 501, 532, 962, 1485, 2177
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 5 cut(s) 265, 709, 1658, 2243, 2891
BseMI GCAATG 2 cut(s) 1256, 2566
BseMII CTCAG 7 cut(s) 149, 282, 407, 1739, 2097, 2681, 2793
BseNI ACTGG 5 cut(s) 766, 1244, 1535, 1821, 2828
BseSI GKGCMC 1 cut(s) 2752
BseXI GCAGC 6 cut(s) 142, 310, 536, 2319, 2696, 2707
BsgI GTGCAG 2 cut(s) 543, 2105
Bsh1236I CGCG 1 cut(s) 2436
BshNI GGYRCC 1 cut(s) 2749
BshVI ATCGAT 2 cut(s) 2577, 2853
BsiHKAI GWGCWC 1 cut(s) 1053
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 794, 1617, 2662
BslI CCNNNNNNNGG 5 cut(s) 265, 709, 1658, 2243, 2891
BsmAI GTCTC 3 cut(s) 829, 1145, 1506
BsmFI GGGAC 3 cut(s) 794, 1617, 2662
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 1053, 1377, 1418, 2752
Bsp1407I TGTACA 1 cut(s) 551
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 2 cut(s) 2434, 2436
BspCNI CTCAG 7 cut(s) 148, 283, 406, 1738, 2098, 2682, 2794
BspDI ATCGAT 2 cut(s) 2577, 2853
BspFNI CGCG 1 cut(s) 2436
BspLI GGNNCC 6 cut(s) 1417, 1648, 2472, 2650, 2651, 2751
BspMAI CTGCAG 2 cut(s) 328, 2445
BspPI GGATC 4 cut(s) 68, 95, 934, 2110
BspT104I TTCGAA 1 cut(s) 730
BspT107I GGYRCC 1 cut(s) 2749
BspTI CTTAAG 1 cut(s) 1505
BsrDI GCAATG 2 cut(s) 1256, 2566
BsrGI TGTACA 1 cut(s) 551
BsrI ACTGG 5 cut(s) 766, 1244, 1535, 1821, 2828
BssECI CCNNGG 3 cut(s) 162, 1376, 2434
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 4 cut(s) 203, 1378, 2291, 2475
Bst4CI ACNGT 6 cut(s) 808, 1740, 1825, 2072, 2464, 2845
Bst6I CTCTTC 2 cut(s) 12, 366
BstAFI CTTAAG 1 cut(s) 1505
BstAPI GCANNNNNTGC 1 cut(s) 2681
BstAUI TGTACA 1 cut(s) 551
BstBAI YACGTR 4 cut(s) 1195, 1475, 2125, 2398
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 4 cut(s) 1042, 1073, 1843, 1935
BstDSI CCRYGG 2 cut(s) 162, 2434
BstENI CCTNNNNNAGG 3 cut(s) 707, 1656, 2241
BstF5I GGATG 6 cut(s) 172, 501, 532, 962, 1485, 2177
BstFNI CGCG 1 cut(s) 2436
BstMAI GTCTC 3 cut(s) 829, 1145, 1506
BstMWI GCNNNNNNNGC 9 cut(s) 207, 320, 511, 1303, 1721, 1730, 1930, 2593, 2681
BstNI CCWGG 4 cut(s) 203, 1378, 2291, 2475
BstNSI RCATGY 2 cut(s) 1057, 1369
BstSCI CCNGG 4 cut(s) 201, 1376, 2289, 2473
BstSFI CTRYAG 3 cut(s) 324, 1961, 2441
BstSLI GKGCMC 1 cut(s) 2752
BstSNI TACGTA 1 cut(s) 2398
BstUI CGCG 1 cut(s) 2436
BstV1I GCAGC 6 cut(s) 142, 310, 536, 2319, 2696, 2707
BstX2I RGATCY 2 cut(s) 926, 2023
BstXI CCANNNNNNTGG 1 cut(s) 1643
BstYI RGATCY 2 cut(s) 926, 2023
Bsu15I ATCGAT 2 cut(s) 2577, 2853
BsuI GTATCC 3 cut(s) 559, 595, 2441
BsuTUI ATCGAT 2 cut(s) 2577, 2853
BtgI CCRYGG 2 cut(s) 162, 2434
BtsCI GGATG 6 cut(s) 172, 501, 532, 962, 1485, 2177
BtsIMutI CAGTG 6 cut(s) 178, 288, 2469, 2687, 2835, 2841
Cac8I GCNNGC 4 cut(s) 1042, 1073, 1843, 1935
Cfr13I GGNCC 6 cut(s) 208, 881, 1966, 2058, 2471, 2649
Cfr42I CCGCGG 1 cut(s) 2437
ClaI ATCGAT 2 cut(s) 2577, 2853
DraI TTTAAA 2 cut(s) 1015, 1849
EaeI YGGCCR 2 cut(s) 910, 1976
Eam1104I CTCTTC 2 cut(s) 12, 366
EarI CTCTTC 2 cut(s) 12, 366
Eco105I TACGTA 1 cut(s) 2398
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 2 cut(s) 702, 2247
Eco24I GRGCYC 2 cut(s) 1377, 1418
Eco47I GGWCC 3 cut(s) 1966, 2471, 2649
Eco57I CTGAAG 5 cut(s) 491, 1255, 1674, 2121, 2312
EcoNI CCTNNNNNAGG 3 cut(s) 707, 1656, 2241
EcoO109I RGGNCCY 3 cut(s) 208, 2058, 2649
EcoRI GAATTC 3 cut(s) 1422, 2266, 2549
EcoRII CCWGG 4 cut(s) 201, 1376, 2289, 2473
EcoT14I CCWWGG 1 cut(s) 162
EcoT22I ATGCAT 2 cut(s) 660, 2812
EcoT38I GRGCYC 2 cut(s) 1377, 1418
ErhI CCWWGG 1 cut(s) 162
FalI AAGNNNNNCTT 4 cut(s) 669, 701, 2894, 2926
FaqI GGGAC 3 cut(s) 794, 1617, 2662
FauNDI CATATG 1 cut(s) 231
FbaI TGATCA 1 cut(s) 1875
FblI GTMKAC 1 cut(s) 2067
Fnu4HI GCNGC 6 cut(s) 156, 324, 525, 2333, 2685, 2696
FokI GGATG 6 cut(s) 179, 508, 519, 969, 1472, 2164
FriOI GRGCYC 2 cut(s) 1377, 1418
Fsp4HI GCNGC 6 cut(s) 156, 324, 525, 2333, 2685, 2696
FspBI CTAG 2 cut(s) 246, 1994
GluI GCNGC 6 cut(s) 156, 324, 525, 2333, 2685, 2696
GsuI CTGGAG 5 cut(s) 224, 511, 809, 840, 1402
HindIII AAGCTT 5 cut(s) 1304, 1843, 2207, 2525, 2585
HphI GGTGA 7 cut(s) 776, 857, 992, 1024, 1367, 1545, 1789
Hpy166II GTNNAC 3 cut(s) 2068, 2281, 2774
Hpy8I GTNNAC 3 cut(s) 2068, 2281, 2774
HpyCH4III ACNGT 6 cut(s) 808, 1740, 1825, 2072, 2464, 2845
HpyCH4IV ACGT 6 cut(s) 1194, 1474, 1611, 2124, 2397, 2513
HpyF10VI GCNNNNNNNGC 9 cut(s) 207, 320, 511, 1303, 1721, 1730, 1930, 2593, 2681
HpySE526I ACGT 6 cut(s) 1194, 1474, 1611, 2124, 2397, 2513
KflI GGGWCCC 1 cut(s) 2649
Ksp22I TGATCA 1 cut(s) 1875
KspI CCGCGG 1 cut(s) 2437
LmnI GCTCC 3 cut(s) 1421, 1652, 2828
Lsp1109I GCAGC 6 cut(s) 142, 310, 536, 2319, 2696, 2707
LweI GCATC 8 cut(s) 415, 523, 667, 1027, 1348, 1448, 2037, 2819
MaeI CTAG 2 cut(s) 246, 1994
MaeII ACGT 6 cut(s) 1194, 1474, 1611, 2124, 2397, 2513
MaeIII GTNAC 9 cut(s) 272, 1095, 1551, 1777, 1888, 2072, 2393, 2398, 2877
MfeI CAATTG 2 cut(s) 559, 1627
MflI RGATCY 2 cut(s) 926, 2023
MhlI GDGCHC 4 cut(s) 1053, 1377, 1418, 2752
MlsI TGGCCA 1 cut(s) 1978
MluNI TGGCCA 1 cut(s) 1978
MlyI GAGTC 5 cut(s) 1346, 1671, 1774, 2117, 2208
MmeI TCCRAC 1 cut(s) 579
Mox20I TGGCCA 1 cut(s) 1978
Mph1103I ATGCAT 2 cut(s) 660, 2812
MroXI GAANNNNTTC 1 cut(s) 2001
MscI TGGCCA 1 cut(s) 1978
MslI CAYNNNNRTG 5 cut(s) 519, 873, 955, 1641, 2301
Msp20I TGGCCA 1 cut(s) 1978
MspA1I CMGCKG 4 cut(s) 323, 422, 2332, 2436
MspCI CTTAAG 1 cut(s) 1505
MspR9I CCNGG 4 cut(s) 203, 1378, 2291, 2475
MunI CAATTG 2 cut(s) 559, 1627
MvaI CCWGG 4 cut(s) 203, 1378, 2291, 2475
MvnI CGCG 1 cut(s) 2436
MwoI GCNNNNNNNGC 9 cut(s) 207, 320, 511, 1303, 1721, 1730, 1930, 2593, 2681
NcoI CCATGG 1 cut(s) 162
NdeI CATATG 1 cut(s) 231
NlaIV GGNNCC 6 cut(s) 1417, 1648, 2472, 2650, 2651, 2751
NmuCI GTSAC 4 cut(s) 1551, 1777, 1888, 2877
NsiI ATGCAT 2 cut(s) 660, 2812
NspI RCATGY 2 cut(s) 1057, 1369
NspV TTCGAA 1 cut(s) 730
PceI AGGCCT 2 cut(s) 702, 2247
PciI ACATGT 1 cut(s) 1365
PdmI GAANNNNTTC 1 cut(s) 2001
PfeI GAWTC 7 cut(s) 455, 529, 793, 983, 989, 1326, 2788
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2473
PkrI GCNGC 6 cut(s) 157, 325, 526, 2334, 2686, 2697
PleI GAGTC 5 cut(s) 1345, 1670, 1774, 2116, 2207
PpsI GAGTC 5 cut(s) 1345, 1670, 1774, 2116, 2207
Ppu21I YACGTR 4 cut(s) 1195, 1475, 2125, 2398
PpuMI RGGWCCY 1 cut(s) 2649
PscI ACATGT 1 cut(s) 1365
PshBI ATTAAT 3 cut(s) 354, 1188, 1955
PsiI TTATAA 2 cut(s) 1794, 2727
Psp1406I AACGTT 1 cut(s) 2513
Psp5II RGGWCCY 1 cut(s) 2649
Psp6I CCWGG 4 cut(s) 201, 1376, 2289, 2473
PspGI CCWGG 4 cut(s) 201, 1376, 2289, 2473
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1417, 1648, 2472, 2650, 2651, 2751
PspPI GGNCC 6 cut(s) 208, 881, 1966, 2058, 2471, 2649
PspPPI RGGWCCY 1 cut(s) 2649
PstI CTGCAG 2 cut(s) 328, 2445
PsuI RGATCY 2 cut(s) 926, 2023
PvuII CAGCTG 3 cut(s) 323, 422, 2332
RseI CAYNNNNRTG 5 cut(s) 519, 873, 955, 1641, 2301
SacII CCGCGG 1 cut(s) 2437
SatI GCNGC 6 cut(s) 156, 324, 525, 2333, 2685, 2696
Sau96I GGNCC 6 cut(s) 208, 881, 1966, 2058, 2471, 2649
ScaI AGTACT 1 cut(s) 2098
SchI GAGTC 5 cut(s) 1346, 1671, 1774, 2117, 2208
ScrFI CCNGG 4 cut(s) 203, 1378, 2291, 2475
SduI GDGCHC 4 cut(s) 1053, 1377, 1418, 2752
SfaNI GCATC 8 cut(s) 415, 523, 667, 1027, 1348, 1448, 2037, 2819
SfcI CTRYAG 3 cut(s) 324, 1961, 2441
SfiI GGCCNNNNNGGCC 1 cut(s) 207
Sfr303I CCGCGG 1 cut(s) 2437
SfuI TTCGAA 1 cut(s) 730
SgrBI CCGCGG 1 cut(s) 2437
SinI GGWCC 3 cut(s) 1966, 2471, 2649
SmiMI CAYNNNNRTG 5 cut(s) 519, 873, 955, 1641, 2301
SmlI CTYRAG 6 cut(s) 224, 1505, 2053, 2588, 2605, 2654
SmoI CTYRAG 6 cut(s) 224, 1505, 2053, 2588, 2605, 2654
SnaBI TACGTA 1 cut(s) 2398
SseBI AGGCCT 2 cut(s) 702, 2247
SsiI CCGC 2 cut(s) 2434, 2436
SspI AATATT 2 cut(s) 1204, 1675
SspMI CTAG 2 cut(s) 246, 1994
StuI AGGCCT 2 cut(s) 702, 2247
StyD4I CCNGG 4 cut(s) 201, 1376, 2289, 2473
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 6 cut(s) 808, 1740, 1825, 2072, 2464, 2845
TaiI ACGT 6 cut(s) 1197, 1477, 1614, 2127, 2400, 2516
TaqI TCGA 7 cut(s) 278, 580, 730, 1371, 2299, 2577, 2853
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 6 cut(s) 51, 282, 551, 1740, 1941, 2096
TfiI GAWTC 7 cut(s) 455, 529, 793, 983, 989, 1326, 2788
TscAI CASTG 6 cut(s) 178, 295, 2469, 2694, 2835, 2848
TseFI GTSAC 4 cut(s) 1551, 1777, 1888, 2877
TseI GCWGC 6 cut(s) 155, 323, 524, 2332, 2684, 2695
Tsp45I GTSAC 4 cut(s) 1551, 1777, 1888, 2877
TspGWI ACGGA 3 cut(s) 53, 687, 1508
TspRI CASTG 6 cut(s) 178, 295, 2469, 2694, 2835, 2848
Vha464I CTTAAG 1 cut(s) 1505
VpaK11BI GGWCC 3 cut(s) 1966, 2471, 2649
VspI ATTAAT 3 cut(s) 354, 1188, 1955
XagI CCTNNNNNAGG 3 cut(s) 707, 1656, 2241
XceI RCATGY 2 cut(s) 1057, 1369
XcmI CCANNNNNNNNNTGG 1 cut(s) 1763
XmiI GTMKAC 1 cut(s) 2067
XmnI GAANNNNTTC 1 cut(s) 2001
XspI CTAG 2 cut(s) 246, 1994
ZrmI AGTACT 1 cut(s) 2098
Zsp2I ATGCAT 2 cut(s) 660, 2812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.