Rh3AG246900

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001584
Physical Location & Seq
Forward (+)
26361808 .. 26375296
13489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG246900.1

Sequence Viewer

Length: 2910 bp
ATGGCTGGGGCGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTTCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGGTAGCTACTTGAAGTACATCAGCGAGCATGGAGGAACAGGAAAAGCTTTCACTGCTGCAGAACACACCAGCTATCGTTTCGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGCGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAAATCTGTTGTCCGATGACTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAGTTGGCATACTTTGGCGGTTCGACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTAAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCGTGTAGGTATCTTGGTCACCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGCTGGCTGGTGAATCAGATTCTACTTTGGACTTTTCTTTCTTTGAAGTATATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACGTTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTATTTGTGAGACAAAGTTTCATTATCAGGACAAAATTGAGCCTATTAATTACGTGGTCAGTATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATTGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAAAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGTGAATTTACACCTACCAGCACGTAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTCATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATATACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAACGGTCACAAAGGAGTACCAAAATTACAAATTTGAACAGTCCTATGAGCAGGCTATTTACTATTGCTCATTAGTTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTACTCATCTTGAAGTTGAAGATCTTGCTAATTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCAAGTAGAATATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCGAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTATTTGTTCTTATTGCAAAGGAACCAGCCTTTCACCAACTTAGAATAGTTGAGCAACTTGGTTACATAACTCGTCTTTTACAGAGGAATGATTGTGGTATCCGTGGACTGCAGTTCATTATACAATCCACAGTGAAGGGTCCAGGACGTATTGATTTGAGAGTGGAGGAATTTCTCAAGACGTTTGAGAGTAAGCTTTATGAGATGACAAATAACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACCAGAACTTGAGGGAAGAAGCTGAATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGCTCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTTCAATGAGCATATAAAAGTTGGGGCACCTCATAAGAGGACATTGAGTGTACGAGTATATGGGAATCGTCATTCATTTGAGTATGCATCAGATAAAAGATCCCTAGTGCAACCTTGCACCGTCAAAATCGATGATATTTTCGCTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAGCTCCGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

969

Amino Acids

111.79

Weight (kDa)

6.31

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 5.9e-33 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 3.1e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 659 6.6e-103 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 664 - 850 1.2e-09 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 775 - 870 5e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1773
AccB1I GGYRCC 1 cut(s) 2728
AciI CCGC 1 cut(s) 542
AclWI GGATC 5 cut(s) 68, 95, 901, 2089, 2796
AcoI YGGCCR 2 cut(s) 877, 1955
AcuI CTGAAG 4 cut(s) 458, 1222, 2100, 2291
AfiI CCNNNNNNNGG 8 cut(s) 232, 233, 456, 676, 841, 1349, 2222, 2870
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AhlI ACTAGT 1 cut(s) 1649
AjnI CCWGG 4 cut(s) 168, 1343, 2268, 2452
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw26I GTCTC 2 cut(s) 1112, 1473
AlwI GGATC 5 cut(s) 68, 95, 901, 2089, 2796
ApeKI GCWGC 5 cut(s) 122, 290, 491, 2663, 2674
AseI ATTAAT 2 cut(s) 321, 1155
AspS9I GGNCC 6 cut(s) 175, 848, 1945, 2037, 2450, 2628
AsuC2I CCSGG 1 cut(s) 1631
AsuHPI GGTGA 9 cut(s) 743, 824, 860, 959, 991, 1334, 1524, 1768, 2336
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1945, 2450, 2628
BaeGI GKGCMC 1 cut(s) 2731
BalI TGGCCA 1 cut(s) 1957
BanI GGYRCC 1 cut(s) 2728
BanII GRGCYC 2 cut(s) 1344, 1385
BbvCI CCTCAGC 1 cut(s) 1704
BbvI GCAGC 5 cut(s) 109, 277, 503, 2675, 2686
BccI CCATC 6 cut(s) 158, 323, 918, 1957, 2164, 2618
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 4 cut(s) 170, 1345, 2270, 2454
BciVI GTATCC 2 cut(s) 562, 2420
BclI TGATCA 1 cut(s) 1854
BcnI CCSGG 1 cut(s) 1631
BcoDI GTCTC 2 cut(s) 1112, 1473
BcuI ACTAGT 1 cut(s) 1649
BfaI CTAG 4 cut(s) 213, 1650, 1973, 2807
BfmI CTRYAG 4 cut(s) 291, 396, 1940, 2420
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 2 cut(s) 562, 2420
BglI GCCNNNNNGGC 1 cut(s) 174
BglII AGATCT 1 cut(s) 2002
BisI GCNGC 5 cut(s) 123, 291, 492, 2664, 2675
BlsI GCNGC 5 cut(s) 124, 292, 493, 2665, 2676
BmcAI AGTACT 1 cut(s) 2077
Bme1390I CCNGG 5 cut(s) 170, 1345, 1631, 2270, 2454
Bme18I GGWCC 3 cut(s) 1945, 2450, 2628
BmgT120I GGNCC 6 cut(s) 175, 848, 1945, 2037, 2450, 2628
BmiI GGNNCC 7 cut(s) 1384, 1627, 2334, 2451, 2629, 2630, 2730
BmrFI CCNGG 5 cut(s) 170, 1345, 1631, 2270, 2454
BmsI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2016, 2798
BpmI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1704
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 6 cut(s) 212, 2017, 2471, 2588, 2605, 2618
BpuMI CCSGG 1 cut(s) 1631
Bsa29I ATCGAT 2 cut(s) 2556, 2832
BsaAI YACGTR 2 cut(s) 1162, 1442
BsaJI CCNNGG 5 cut(s) 129, 1343, 1458, 1629, 2413
BsaWI WCCGGW 1 cut(s) 2891
Bsc4I CCNNNNNNNGG 8 cut(s) 232, 233, 456, 676, 841, 1349, 2222, 2870
Bse1I ACTGG 5 cut(s) 461, 733, 1211, 1514, 1800
Bse3DI GCAATG 2 cut(s) 1223, 2545
BseBI CCWGG 4 cut(s) 170, 1345, 2270, 2454
BseCI ATCGAT 2 cut(s) 2556, 2832
BseDI CCNNGG 5 cut(s) 129, 1343, 1458, 1629, 2413
BseGI GGATG 5 cut(s) 139, 499, 929, 1452, 2156
BseLI CCNNNNNNNGG 8 cut(s) 232, 233, 456, 676, 841, 1349, 2222, 2870
BseMI GCAATG 2 cut(s) 1223, 2545
BseMII CTCAG 4 cut(s) 374, 1695, 2076, 2660
BseNI ACTGG 5 cut(s) 461, 733, 1211, 1514, 1800
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2731
BseXI GCAGC 5 cut(s) 109, 277, 503, 2675, 2686
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 510
BshNI GGYRCC 1 cut(s) 2728
BshVI ATCGAT 2 cut(s) 2556, 2832
BsiSI CCGG 2 cut(s) 1631, 2892
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1596, 2641
BslI CCNNNNNNNGG 8 cut(s) 232, 233, 456, 676, 841, 1349, 2222, 2870
BsmAI GTCTC 2 cut(s) 1112, 1473
BsmFI GGGAC 3 cut(s) 761, 1596, 2641
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 1344, 1385, 2731
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 542
BspCNI CTCAG 4 cut(s) 373, 1696, 2077, 2661
BspDI ATCGAT 2 cut(s) 2556, 2832
BspLI GGNNCC 7 cut(s) 1384, 1627, 2334, 2451, 2629, 2630, 2730
BspMAI CTGCAG 2 cut(s) 295, 2424
BspPI GGATC 5 cut(s) 68, 95, 901, 2089, 2796
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2728
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 2 cut(s) 1223, 2545
BsrI ACTGG 5 cut(s) 461, 733, 1211, 1514, 1800
BssECI CCNNGG 5 cut(s) 129, 1343, 1458, 1629, 2413
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 4 cut(s) 170, 1345, 2270, 2454
Bst4CI ACNGT 7 cut(s) 775, 1719, 1804, 1867, 1902, 2443, 2824
Bst6I CTCTTC 2 cut(s) 12, 333
BstAFI CTTAAG 1 cut(s) 1472
BstAPI GCANNNNNTGC 1 cut(s) 2660
BstBAI YACGTR 2 cut(s) 1162, 1442
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 6 cut(s) 260, 942, 1009, 1040, 1822, 1914
BstDEI CTNAG 7 cut(s) 360, 485, 1704, 2044, 2085, 2351, 2669
BstDSI CCRYGG 3 cut(s) 129, 1458, 2413
BstEII GGTNACC 1 cut(s) 866
BstENI CCTNNNNNAGG 2 cut(s) 674, 2220
BstF5I GGATG 5 cut(s) 139, 499, 929, 1452, 2156
BstMAI GTCTC 2 cut(s) 1112, 1473
BstMWI GCNNNNNNNGC 8 cut(s) 174, 287, 478, 1270, 1700, 1709, 2572, 2660
BstNI CCWGG 4 cut(s) 170, 1345, 2270, 2454
BstNSI RCATGY 2 cut(s) 1024, 1336
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 5 cut(s) 168, 1343, 1629, 2268, 2452
BstSFI CTRYAG 4 cut(s) 291, 396, 1940, 2420
BstSLI GKGCMC 1 cut(s) 2731
BstV1I GCAGC 5 cut(s) 109, 277, 503, 2675, 2686
BstX2I RGATCY 3 cut(s) 893, 2002, 2801
BstXI CCANNNNNNTGG 1 cut(s) 1622
BstYI RGATCY 3 cut(s) 893, 2002, 2801
Bsu15I ATCGAT 2 cut(s) 2556, 2832
BsuI GTATCC 2 cut(s) 562, 2420
BsuTUI ATCGAT 2 cut(s) 2556, 2832
BtgI CCRYGG 3 cut(s) 129, 1458, 2413
BtsCI GGATG 5 cut(s) 139, 499, 929, 1452, 2156
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 4 cut(s) 145, 285, 2448, 2666
Cac8I GCNNGC 6 cut(s) 260, 942, 1009, 1040, 1822, 1914
Cfr13I GGNCC 6 cut(s) 175, 848, 1945, 2037, 2450, 2628
ClaI ATCGAT 2 cut(s) 2556, 2832
DdeI CTNAG 7 cut(s) 360, 485, 1704, 2044, 2085, 2351, 2669
DraI TTTAAA 1 cut(s) 1828
EaeI YGGCCR 2 cut(s) 877, 1955
Eam1104I CTCTTC 2 cut(s) 12, 333
EarI CTCTTC 2 cut(s) 12, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 669, 2226
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco47I GGWCC 3 cut(s) 1945, 2450, 2628
Eco57I CTGAAG 4 cut(s) 458, 1222, 2100, 2291
Eco91I GGTNACC 1 cut(s) 866
EcoNI CCTNNNNNAGG 2 cut(s) 674, 2220
EcoO109I RGGNCCY 3 cut(s) 175, 2037, 2628
EcoO65I GGTNACC 1 cut(s) 866
EcoRI GAATTC 3 cut(s) 1389, 2245, 2528
EcoRII CCWGG 4 cut(s) 168, 1343, 2268, 2452
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 627, 2791
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 3 cut(s) 761, 1596, 2641
FauNDI CATATG 2 cut(s) 198, 1537
FbaI TGATCA 1 cut(s) 1854
Fnu4HI GCNGC 5 cut(s) 123, 291, 492, 2664, 2675
FokI GGATG 5 cut(s) 146, 486, 936, 1439, 2143
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 5 cut(s) 123, 291, 492, 2664, 2675
FspBI CTAG 4 cut(s) 213, 1650, 1973, 2807
GluI GCNGC 5 cut(s) 123, 291, 492, 2664, 2675
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
HapII CCGG 2 cut(s) 1631, 2892
HindIII AAGCTT 6 cut(s) 279, 1271, 1822, 2186, 2504, 2564
HinfI GANTC 9 cut(s) 496, 760, 950, 956, 1293, 1304, 2087, 2178, 2767
HpaII CCGG 2 cut(s) 1631, 2892
HphI GGTGA 9 cut(s) 743, 824, 860, 959, 991, 1334, 1524, 1768, 2336
Hpy166II GTNNAC 3 cut(s) 2260, 2417, 2753
Hpy8I GTNNAC 3 cut(s) 2260, 2417, 2753
HpyCH4III ACNGT 7 cut(s) 775, 1719, 1804, 1867, 1902, 2443, 2824
HpyCH4IV ACGT 6 cut(s) 1053, 1161, 1441, 1590, 2458, 2492
HpyF10VI GCNNNNNNNGC 8 cut(s) 174, 287, 478, 1270, 1700, 1709, 2572, 2660
HpyF3I CTNAG 7 cut(s) 360, 485, 1704, 2044, 2085, 2351, 2669
HpySE526I ACGT 6 cut(s) 1053, 1161, 1441, 1590, 2458, 2492
KflI GGGWCCC 1 cut(s) 2628
Ksp22I TGATCA 1 cut(s) 1854
LmnI GCTCC 3 cut(s) 1388, 1631, 2894
Lsp1109I GCAGC 5 cut(s) 109, 277, 503, 2675, 2686
LweI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2016, 2798
MaeI CTAG 4 cut(s) 213, 1650, 1973, 2807
MaeII ACGT 6 cut(s) 1053, 1161, 1441, 1590, 2458, 2492
MaeIII GTNAC 8 cut(s) 866, 1062, 1530, 1756, 1867, 2051, 2372, 2856
MboII GAAGA 9 cut(s) 29, 350, 611, 1288, 1401, 1825, 2012, 2241, 2603
MfeI CAATTG 1 cut(s) 1606
MflI RGATCY 3 cut(s) 893, 2002, 2801
MhlI GDGCHC 3 cut(s) 1344, 1385, 2731
MlsI TGGCCA 1 cut(s) 1957
MluNI TGGCCA 1 cut(s) 1957
MlyI GAGTC 3 cut(s) 1313, 2096, 2187
Mox20I TGGCCA 1 cut(s) 1957
Mph1103I ATGCAT 2 cut(s) 627, 2791
MscI TGGCCA 1 cut(s) 1957
MslI CAYNNNNRTG 4 cut(s) 486, 922, 1620, 2280
Msp20I TGGCCA 1 cut(s) 1957
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1472
MspI CCGG 2 cut(s) 1631, 2892
MspR9I CCNGG 5 cut(s) 170, 1345, 1631, 2270, 2454
MunI CAATTG 1 cut(s) 1606
MvaI CCWGG 4 cut(s) 170, 1345, 2270, 2454
MwoI GCNNNNNNNGC 8 cut(s) 174, 287, 478, 1270, 1700, 1709, 2572, 2660
NciI CCSGG 1 cut(s) 1631
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 198, 1537
NlaIV GGNNCC 7 cut(s) 1384, 1627, 2334, 2451, 2629, 2630, 2730
NmuCI GTSAC 5 cut(s) 866, 1530, 1756, 1867, 2856
NsiI ATGCAT 2 cut(s) 627, 2791
NspI RCATGY 2 cut(s) 1024, 1336
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 2 cut(s) 669, 2226
PciI ACATGT 1 cut(s) 1332
PfeI GAWTC 6 cut(s) 496, 760, 950, 956, 1293, 2767
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2452
PkrI GCNGC 5 cut(s) 124, 292, 493, 2665, 2676
PleI GAGTC 3 cut(s) 1312, 2095, 2186
PpsI GAGTC 3 cut(s) 1312, 2095, 2186
Ppu21I YACGTR 2 cut(s) 1162, 1442
PpuMI RGGWCCY 1 cut(s) 2628
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 2 cut(s) 321, 1155
PsiI TTATAA 1 cut(s) 1773
Psp5II RGGWCCY 1 cut(s) 2628
Psp6I CCWGG 4 cut(s) 168, 1343, 2268, 2452
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 4 cut(s) 168, 1343, 2268, 2452
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 7 cut(s) 1384, 1627, 2334, 2451, 2629, 2630, 2730
PspPI GGNCC 6 cut(s) 175, 848, 1945, 2037, 2450, 2628
PspPPI RGGWCCY 1 cut(s) 2628
PstI CTGCAG 2 cut(s) 295, 2424
PsuI RGATCY 3 cut(s) 893, 2002, 2801
PvuII CAGCTG 1 cut(s) 389
RseI CAYNNNNRTG 4 cut(s) 486, 922, 1620, 2280
SatI GCNGC 5 cut(s) 123, 291, 492, 2664, 2675
Sau96I GGNCC 6 cut(s) 175, 848, 1945, 2037, 2450, 2628
ScaI AGTACT 1 cut(s) 2077
SchI GAGTC 3 cut(s) 1313, 2096, 2187
ScrFI CCNGG 5 cut(s) 170, 1345, 1631, 2270, 2454
SduI GDGCHC 3 cut(s) 1344, 1385, 2731
SfaNI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2016, 2798
SfcI CTRYAG 4 cut(s) 291, 396, 1940, 2420
SfiI GGCCNNNNNGGCC 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 3 cut(s) 1945, 2450, 2628
SmiMI CAYNNNNRTG 4 cut(s) 486, 922, 1620, 2280
SmlI CTYRAG 7 cut(s) 191, 1472, 2032, 2486, 2567, 2584, 2633
SmoI CTYRAG 7 cut(s) 191, 1472, 2032, 2486, 2567, 2584, 2633
SpeI ACTAGT 1 cut(s) 1649
SseBI AGGCCT 2 cut(s) 669, 2226
SsiI CCGC 1 cut(s) 542
SspI AATATT 1 cut(s) 703
SspMI CTAG 4 cut(s) 213, 1650, 1973, 2807
StuI AGGCCT 2 cut(s) 669, 2226
StyD4I CCNGG 5 cut(s) 168, 1343, 1629, 2268, 2452
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 7 cut(s) 775, 1719, 1804, 1867, 1902, 2443, 2824
TaiI ACGT 6 cut(s) 1056, 1164, 1444, 1593, 2461, 2495
TaqI TCGA 7 cut(s) 315, 547, 697, 2233, 2278, 2556, 2832
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 5 cut(s) 51, 249, 518, 1719, 2075
TfiI GAWTC 6 cut(s) 496, 760, 950, 956, 1293, 2767
TscAI CASTG 4 cut(s) 145, 292, 2448, 2673
TseFI GTSAC 5 cut(s) 866, 1530, 1756, 1867, 2856
TseI GCWGC 5 cut(s) 122, 290, 491, 2663, 2674
Tsp45I GTSAC 5 cut(s) 866, 1530, 1756, 1867, 2856
TspGWI ACGGA 4 cut(s) 53, 654, 1475, 2402
TspRI CASTG 4 cut(s) 145, 292, 2448, 2673
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1945, 2450, 2628
VspI ATTAAT 2 cut(s) 321, 1155
XagI CCTNNNNNAGG 2 cut(s) 674, 2220
XceI RCATGY 2 cut(s) 1024, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1742
XspI CTAG 4 cut(s) 213, 1650, 1973, 2807
ZrmI AGTACT 1 cut(s) 2077
Zsp2I ATGCAT 2 cut(s) 627, 2791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.