Rh3AG245000

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001584
Physical Location & Seq
Reverse (-)
25971713 .. 25993678
21966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG245000.1

Sequence Viewer

Length: 2904 bp
ATGGCTGTGGGGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTCAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATAGCTACTCGAAGTACATCACCGAGCATGGAGGAAGAACAAATGCTTTCACAGCTACAGAACACACTAACTATTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGGATACTTTGGAGGTTCAACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAATATCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACTGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAATGGCCATGTAGGTATCTTTGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTTCTTTCTTTAAAGTAGTTATTGATCTCACCGATGCAGGCCAGGAGCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGTGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAGTTATGTGGTCAATATTTCATCAAATATGCAGATATATCCCCCAAAAGACTGGCTGGTAAGATCGTCATTGCCTTCTAACTTCAGTCCTGCCATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGAGAATTTACACCTACCAGCACGTAATATGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAATGAGAAGGTCAAAATTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTATGGTATAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTTGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTGATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTAGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGCAGTACCAAAATTACAAATTTCAACAGCCTTATGGGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGGAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCATGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCGAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTACACTGAATGTGAAACTTGAGCTGTTTGGTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACATAACTGCTCTTAAACCGAGGAATGATTATGGTATCTGTGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGTGGGAAATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGCTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

967

Amino Acids

111.61

Weight (kDa)

6.01

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 1.6e-37 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 1.1e-30 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 657 8.1e-105 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 662 - 848 5.8e-14 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 772 - 868 2.7e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1767, 2700
AccB1I GGYRCC 1 cut(s) 2722
AclI AACGTT 1 cut(s) 2486
AclWI GGATC 4 cut(s) 68, 95, 901, 2083
AcoI YGGCCR 3 cut(s) 848, 877, 1949
AcuI CTGAAG 3 cut(s) 458, 1222, 2094
AfiI CCNNNNNNNGG 6 cut(s) 232, 456, 676, 841, 1349, 2216
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AhlI ACTAGT 1 cut(s) 1643
AjnI CCWGG 5 cut(s) 168, 1011, 1343, 2262, 2446
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 3 cut(s) 796, 1112, 1473
AlwI GGATC 4 cut(s) 68, 95, 901, 2083
ApeKI GCWGC 4 cut(s) 122, 491, 2657, 2668
AseI ATTAAT 2 cut(s) 321, 1928
Asp700I GAANNNNTTC 1 cut(s) 1974
AspS9I GGNCC 5 cut(s) 175, 1939, 2031, 2444, 2622
AsuC2I CCSGG 1 cut(s) 1625
AsuHPI GGTGA 8 cut(s) 247, 743, 824, 959, 991, 1334, 1518, 1762
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1939, 2444, 2622
BaeGI GKGCMC 1 cut(s) 2725
BalI TGGCCA 2 cut(s) 850, 1951
BanI GGYRCC 1 cut(s) 2722
BanII GRGCYC 2 cut(s) 1344, 1385
Bbv12I GWGCWC 1 cut(s) 1020
BbvCI CCTCAGC 1 cut(s) 1698
BbvI GCAGC 4 cut(s) 109, 503, 2669, 2680
BccI CCATC 7 cut(s) 158, 323, 446, 918, 1951, 2158, 2612
BciT130I CCWGG 5 cut(s) 170, 1013, 1345, 2264, 2448
BciVI GTATCC 2 cut(s) 526, 562
BclI TGATCA 1 cut(s) 1848
BcnI CCSGG 1 cut(s) 1625
BcoDI GTCTC 3 cut(s) 796, 1112, 1473
BcuI ACTAGT 1 cut(s) 1643
BfaI CTAG 4 cut(s) 213, 1644, 1790, 1967
BfmI CTRYAG 3 cut(s) 291, 1934, 2414
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 2 cut(s) 526, 562
BglI GCCNNNNNGGC 2 cut(s) 174, 1903
BglII AGATCT 1 cut(s) 1996
BisI GCNGC 4 cut(s) 123, 492, 2658, 2669
BlsI GCNGC 4 cut(s) 124, 493, 2659, 2670
BmcAI AGTACT 1 cut(s) 2071
Bme1390I CCNGG 6 cut(s) 170, 1013, 1345, 1625, 2264, 2448
Bme18I GGWCC 3 cut(s) 1939, 2444, 2622
BmgT120I GGNCC 5 cut(s) 175, 1939, 2031, 2444, 2622
BmiI GGNNCC 6 cut(s) 1384, 2060, 2445, 2623, 2624, 2724
BmrFI CCNGG 6 cut(s) 170, 1013, 1345, 1625, 2264, 2448
BmrI ACTGGG 1 cut(s) 2795
BmsI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2010, 2792
BmuI ACTGGG 1 cut(s) 2795
BpmI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1698
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 6 cut(s) 212, 2011, 2321, 2582, 2599, 2612
BpuMI CCSGG 1 cut(s) 1625
Bsa29I ATCGAT 2 cut(s) 2550, 2826
BsaAI YACGTR 1 cut(s) 1442
BsaJI CCNNGG 5 cut(s) 129, 1343, 1458, 1623, 2387
Bsc4I CCNNNNNNNGG 6 cut(s) 232, 456, 676, 841, 1349, 2216
Bse1I ACTGG 4 cut(s) 733, 1211, 1508, 2801
Bse3DI GCAATG 2 cut(s) 1223, 2539
BseBI CCWGG 5 cut(s) 170, 1013, 1345, 2264, 2448
BseCI ATCGAT 2 cut(s) 2550, 2826
BseDI CCNNGG 5 cut(s) 129, 1343, 1458, 1623, 2387
BseGI GGATG 5 cut(s) 139, 499, 929, 1452, 2150
BseLI CCNNNNNNNGG 6 cut(s) 232, 456, 676, 841, 1349, 2216
BseMI GCAATG 2 cut(s) 1223, 2539
BseMII CTCAG 5 cut(s) 374, 1689, 2070, 2654, 2766
BseNI ACTGG 4 cut(s) 733, 1211, 1508, 2801
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2725
BseXI GCAGC 4 cut(s) 109, 503, 2669, 2680
BsgI GTGCAG 1 cut(s) 510
BshNI GGYRCC 1 cut(s) 2722
BshVI ATCGAT 2 cut(s) 2550, 2826
BsiHKAI GWGCWC 1 cut(s) 1020
BsiSI CCGG 1 cut(s) 1625
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1590, 2635
BslI CCNNNNNNNGG 6 cut(s) 232, 456, 676, 841, 1349, 2216
BsmAI GTCTC 3 cut(s) 796, 1112, 1473
BsmFI GGGAC 3 cut(s) 761, 1590, 2635
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 4 cut(s) 1020, 1344, 1385, 2725
Bsp19I CCATGG 1 cut(s) 129
BspCNI CTCAG 5 cut(s) 373, 1690, 2071, 2655, 2767
BspDI ATCGAT 2 cut(s) 2550, 2826
BspLI GGNNCC 6 cut(s) 1384, 2060, 2445, 2623, 2624, 2724
BspMAI CTGCAG 1 cut(s) 2418
BspPI GGATC 4 cut(s) 68, 95, 901, 2083
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2722
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 2 cut(s) 1223, 2539
BsrI ACTGG 4 cut(s) 733, 1211, 1508, 2801
BssECI CCNNGG 5 cut(s) 129, 1343, 1458, 1623, 2387
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 5 cut(s) 170, 1013, 1345, 2264, 2448
Bst4CI ACNGT 5 cut(s) 775, 1713, 1798, 2437, 2818
Bst6I CTCTTC 2 cut(s) 12, 333
BstAFI CTTAAG 1 cut(s) 1472
BstAPI GCANNNNNTGC 1 cut(s) 2654
BstBAI YACGTR 1 cut(s) 1442
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 4 cut(s) 1009, 1040, 1816, 1908
BstDEI CTNAG 9 cut(s) 360, 485, 1698, 2004, 2038, 2079, 2345, 2663, 2775
BstDSI CCRYGG 2 cut(s) 129, 1458
BstENI CCTNNNNNAGG 2 cut(s) 674, 2214
BstF5I GGATG 5 cut(s) 139, 499, 929, 1452, 2150
BstMAI GTCTC 3 cut(s) 796, 1112, 1473
BstMWI GCNNNNNNNGC 9 cut(s) 174, 287, 478, 1270, 1694, 1703, 1903, 2566, 2654
BstNI CCWGG 5 cut(s) 170, 1013, 1345, 2264, 2448
BstNSI RCATGY 3 cut(s) 1024, 1336, 2099
BstSCI CCNGG 6 cut(s) 168, 1011, 1343, 1623, 2262, 2446
BstSFI CTRYAG 3 cut(s) 291, 1934, 2414
BstSLI GKGCMC 1 cut(s) 2725
BstV1I GCAGC 4 cut(s) 109, 503, 2669, 2680
BstX2I RGATCY 2 cut(s) 893, 1996
BstXI CCANNNNNNTGG 1 cut(s) 1206
BstYI RGATCY 2 cut(s) 893, 1996
Bsu15I ATCGAT 2 cut(s) 2550, 2826
BsuI GTATCC 2 cut(s) 526, 562
BsuTUI ATCGAT 2 cut(s) 2550, 2826
BtgI CCRYGG 2 cut(s) 129, 1458
BtsCI GGATG 5 cut(s) 139, 499, 929, 1452, 2150
BtsIMutI CAGTG 6 cut(s) 145, 2285, 2442, 2660, 2808, 2814
Cac8I GCNNGC 4 cut(s) 1009, 1040, 1816, 1908
Cfr13I GGNCC 5 cut(s) 175, 1939, 2031, 2444, 2622
ClaI ATCGAT 2 cut(s) 2550, 2826
DdeI CTNAG 9 cut(s) 360, 485, 1698, 2004, 2038, 2079, 2345, 2663, 2775
DraI TTTAAA 2 cut(s) 982, 1822
EaeI YGGCCR 3 cut(s) 848, 877, 1949
Eam1104I CTCTTC 2 cut(s) 12, 333
EarI CTCTTC 2 cut(s) 12, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 669, 2220
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco47I GGWCC 3 cut(s) 1939, 2444, 2622
Eco57I CTGAAG 3 cut(s) 458, 1222, 2094
EcoNI CCTNNNNNAGG 2 cut(s) 674, 2214
EcoO109I RGGNCCY 3 cut(s) 175, 2031, 2622
EcoRI GAATTC 3 cut(s) 1389, 2239, 2522
EcoRII CCWGG 5 cut(s) 168, 1011, 1343, 2262, 2446
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 627, 2785
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 4 cut(s) 636, 668, 2867, 2899
FaqI GGGAC 3 cut(s) 761, 1590, 2635
FauNDI CATATG 2 cut(s) 198, 1531
FbaI TGATCA 1 cut(s) 1848
Fnu4HI GCNGC 4 cut(s) 123, 492, 2658, 2669
FokI GGATG 5 cut(s) 146, 486, 936, 1439, 2137
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 4 cut(s) 123, 492, 2658, 2669
FspBI CTAG 4 cut(s) 213, 1644, 1790, 1967
GluI GCNGC 4 cut(s) 123, 492, 2658, 2669
GsuI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
HapII CCGG 1 cut(s) 1625
HindIII AAGCTT 5 cut(s) 1271, 1816, 2180, 2498, 2558
HpaII CCGG 1 cut(s) 1625
HphI GGTGA 8 cut(s) 247, 743, 824, 959, 991, 1334, 1518, 1762
Hpy166II GTNNAC 3 cut(s) 2254, 2411, 2747
Hpy8I GTNNAC 3 cut(s) 2254, 2411, 2747
HpyCH4III ACNGT 5 cut(s) 775, 1713, 1798, 2437, 2818
HpyCH4IV ACGT 3 cut(s) 1441, 1584, 2486
HpyF10VI GCNNNNNNNGC 9 cut(s) 174, 287, 478, 1270, 1694, 1703, 1903, 2566, 2654
HpyF3I CTNAG 9 cut(s) 360, 485, 1698, 2004, 2038, 2079, 2345, 2663, 2775
HpySE526I ACGT 3 cut(s) 1441, 1584, 2486
KflI GGGWCCC 1 cut(s) 2622
Ksp22I TGATCA 1 cut(s) 1848
LmnI GCTCC 4 cut(s) 1015, 1388, 1625, 2801
Lsp1109I GCAGC 4 cut(s) 109, 503, 2669, 2680
LweI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2010, 2792
MaeI CTAG 4 cut(s) 213, 1644, 1790, 1967
MaeII ACGT 3 cut(s) 1441, 1584, 2486
MaeIII GTNAC 7 cut(s) 1062, 1524, 1750, 1861, 2045, 2366, 2850
MfeI CAATTG 2 cut(s) 526, 1600
MflI RGATCY 2 cut(s) 893, 1996
MhlI GDGCHC 4 cut(s) 1020, 1344, 1385, 2725
MlsI TGGCCA 2 cut(s) 850, 1951
MluNI TGGCCA 2 cut(s) 850, 1951
MlyI GAGTC 3 cut(s) 1313, 2090, 2181
Mox20I TGGCCA 2 cut(s) 850, 1951
Mph1103I ATGCAT 2 cut(s) 627, 2785
MroXI GAANNNNTTC 1 cut(s) 1974
MscI TGGCCA 2 cut(s) 850, 1951
MslI CAYNNNNRTG 3 cut(s) 486, 922, 2274
Msp20I TGGCCA 2 cut(s) 850, 1951
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1472
MspI CCGG 1 cut(s) 1625
MspR9I CCNGG 6 cut(s) 170, 1013, 1345, 1625, 2264, 2448
MunI CAATTG 2 cut(s) 526, 1600
MvaI CCWGG 5 cut(s) 170, 1013, 1345, 2264, 2448
MwoI GCNNNNNNNGC 9 cut(s) 174, 287, 478, 1270, 1694, 1703, 1903, 2566, 2654
NciI CCSGG 1 cut(s) 1625
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 198, 1531
NlaIV GGNNCC 6 cut(s) 1384, 2060, 2445, 2623, 2624, 2724
NmuCI GTSAC 4 cut(s) 1524, 1750, 1861, 2850
NsiI ATGCAT 2 cut(s) 627, 2785
NspI RCATGY 3 cut(s) 1024, 1336, 2099
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 2 cut(s) 669, 2220
PciI ACATGT 1 cut(s) 1332
PdmI GAANNNNTTC 1 cut(s) 1974
PfeI GAWTC 7 cut(s) 422, 496, 760, 950, 956, 1293, 2761
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2446
PkrI GCNGC 4 cut(s) 124, 493, 2659, 2670
PleI GAGTC 3 cut(s) 1312, 2089, 2180
PpsI GAGTC 3 cut(s) 1312, 2089, 2180
Ppu21I YACGTR 1 cut(s) 1442
PpuMI RGGWCCY 1 cut(s) 2622
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 2 cut(s) 321, 1928
PsiI TTATAA 2 cut(s) 1767, 2700
Psp1406I AACGTT 1 cut(s) 2486
Psp5II RGGWCCY 1 cut(s) 2622
Psp6I CCWGG 5 cut(s) 168, 1011, 1343, 2262, 2446
PspGI CCWGG 5 cut(s) 168, 1011, 1343, 2262, 2446
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1384, 2060, 2445, 2623, 2624, 2724
PspPI GGNCC 5 cut(s) 175, 1939, 2031, 2444, 2622
PspPPI RGGWCCY 1 cut(s) 2622
PstI CTGCAG 1 cut(s) 2418
PsuI RGATCY 2 cut(s) 893, 1996
PvuII CAGCTG 1 cut(s) 389
RseI CAYNNNNRTG 3 cut(s) 486, 922, 2274
SatI GCNGC 4 cut(s) 123, 492, 2658, 2669
Sau96I GGNCC 5 cut(s) 175, 1939, 2031, 2444, 2622
ScaI AGTACT 1 cut(s) 2071
SchI GAGTC 3 cut(s) 1313, 2090, 2181
ScrFI CCNGG 6 cut(s) 170, 1013, 1345, 1625, 2264, 2448
SduI GDGCHC 4 cut(s) 1020, 1344, 1385, 2725
SfaNI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 2010, 2792
SfcI CTRYAG 3 cut(s) 291, 1934, 2414
SfiI GGCCNNNNNGGCC 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 3 cut(s) 1939, 2444, 2622
SmiMI CAYNNNNRTG 3 cut(s) 486, 922, 2274
SmlI CTYRAG 7 cut(s) 191, 1472, 2026, 2300, 2561, 2578, 2627
SmoI CTYRAG 7 cut(s) 191, 1472, 2026, 2300, 2561, 2578, 2627
SpeI ACTAGT 1 cut(s) 1643
SseBI AGGCCT 2 cut(s) 669, 2220
SspI AATATT 2 cut(s) 703, 1171
SspMI CTAG 4 cut(s) 213, 1644, 1790, 1967
StuI AGGCCT 2 cut(s) 669, 2220
StyD4I CCNGG 6 cut(s) 168, 1011, 1343, 1623, 2262, 2446
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 5 cut(s) 775, 1713, 1798, 2437, 2818
TaiI ACGT 3 cut(s) 1444, 1587, 2489
TaqI TCGA 6 cut(s) 245, 697, 2227, 2272, 2550, 2826
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 6 cut(s) 51, 249, 518, 1713, 1914, 2069
TfiI GAWTC 7 cut(s) 422, 496, 760, 950, 956, 1293, 2761
TscAI CASTG 6 cut(s) 145, 2292, 2442, 2667, 2808, 2821
TseFI GTSAC 4 cut(s) 1524, 1750, 1861, 2850
TseI GCWGC 4 cut(s) 122, 491, 2657, 2668
Tsp45I GTSAC 4 cut(s) 1524, 1750, 1861, 2850
TspGWI ACGGA 3 cut(s) 53, 654, 1475
TspRI CASTG 6 cut(s) 145, 2292, 2442, 2667, 2808, 2821
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1939, 2444, 2622
VspI ATTAAT 2 cut(s) 321, 1928
XagI CCTNNNNNAGG 2 cut(s) 674, 2214
XceI RCATGY 3 cut(s) 1024, 1336, 2099
XcmI CCANNNNNNNNNTGG 2 cut(s) 1255, 1736
XmnI GAANNNNTTC 1 cut(s) 1974
XspI CTAG 4 cut(s) 213, 1644, 1790, 1967
ZrmI AGTACT 1 cut(s) 2071
Zsp2I ATGCAT 2 cut(s) 627, 2785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.