Rroxscaffold_6G00400000

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001584
Physical Location & Seq
Reverse (-)
22057513 .. 22071969
14457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400000.1

Sequence Viewer

Length: 2817 bp
ATGGCTGGGGCGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTAACAAGGCAACTGCTCTTTCATCTCAGACTTGTTTCTCTGTTTCAGTGGATACGTTGCTATGCAGGGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGACAGCTACTTGAAGTACATCATCGAGCATGGAGGAAGAGGAAATGGTTTCACTTATGAAGAACACACCAGCTATCGTTTCGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAATTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGCGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAACTCTGTTGTCCGATGGCTGGAGAATGAACCAGGCGATGGGTTCTAGTTGTTTTTGGGTTTTCTCTCACAAGTATGATGTTCTAATTCTAGATGCACCATGCAATCGGTATACTTTGTGGGTTCGACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCATTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTAAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCGTGCATGTATCTTGGTCACCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGCGGGCTGGTGAATCATATTCTACTTTGGACTTTTCTTTCTTTGAAGTAGATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACGTTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATTGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAAAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGTGAATTTACACCTACCAGCACGTAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTGATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATAAACCGCACACAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAACGGTCACAAAGGAGTACCAAAATTACAAATTTGAACAGTCCTATGAGCAGGCTATTTACTATTGCTCATTAGTTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTACTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTATTTGTTCTTATTGCAAAGCAACCAGCCTTTCACCAACTTAGAATAGTTGAGCAACTTGGTTACATAACTCGTCTTTCACAGAGGAATGATTGTGGTATCCGTGGACTGCAGTTCATTATACAATCCACAGTGAAGGGTCCAGGACGTATTGATTTGAGAGTGGAGGAATTTCTCAAGACGTTTGAGAGTAAGCTTTATGAGATGACAAATAACGAATTCAAGAGCAATGTAAATACTTTGATCGATATTAAGCTTGAGAAGCACCAGAACTTGAGGGAAGAAGCTGAATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTTCAATGAGCGTATAAAAGTGGGGGCACCTCATAAGAGGACATTGAGTGTACGAGTATATGGGAATCGTCATTCATTTGAGTATGCATCAGATAAAAGATCCCTAGTGCAACCTTGCACCGTCAAAATCGATGATATTTTCGCTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAGCTCCGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

938

Amino Acids

108.5

Weight (kDa)

6.48

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 60 - 153 2.2e-16 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 367 3e-28 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 367 - 628 8.5e-94 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 633 - 819 4.1e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 744 - 839 3.1e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1680
AccB1I GGYRCC 1 cut(s) 2635
AccB7I CCANNNNNTGG 1 cut(s) 460
AccI GTMKAC 1 cut(s) 533
AciI CCGC 2 cut(s) 940, 1642
AclWI GGATC 5 cut(s) 68, 95, 901, 1996, 2703
AcoI YGGCCR 2 cut(s) 877, 1862
AcuI CTGAAG 3 cut(s) 1135, 2007, 2198
AdeI CACNNNGTG 1 cut(s) 1652
AfaI GTAC 9 cut(s) 37, 53, 209, 236, 1460, 1628, 1787, 1984, 2661
AfiI CCNNNNNNNGG 7 cut(s) 217, 441, 460, 841, 1262, 2129, 2777
AflII CTTAAG 1 cut(s) 1385
AflIII ACRYGT 1 cut(s) 1245
AhlI ACTAGT 1 cut(s) 1556
AjnI CCWGG 4 cut(s) 453, 1256, 2175, 2359
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw26I GTCTC 1 cut(s) 1386
AlwI GGATC 5 cut(s) 68, 95, 901, 1996, 2703
AoxI GGCC 7 cut(s) 812, 848, 877, 1009, 1862, 1944, 2131
ApeKI GCWGC 2 cut(s) 2570, 2581
AseI ATTAAT 1 cut(s) 306
AspS9I GGNCC 5 cut(s) 848, 1852, 1944, 2357, 2535
AsuC2I CCSGG 1 cut(s) 1538
AsuHPI GGTGA 9 cut(s) 743, 824, 860, 959, 991, 1247, 1431, 1675, 2243
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1852, 2357, 2535
BaeGI GKGCMC 1 cut(s) 2638
BalI TGGCCA 1 cut(s) 1864
BanI GGYRCC 1 cut(s) 2635
BanII GRGCYC 2 cut(s) 1257, 1298
BbvCI CCTCAGC 1 cut(s) 1611
BbvI GCAGC 2 cut(s) 2582, 2593
BccI CCATC 7 cut(s) 308, 431, 454, 918, 1864, 2071, 2525
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 4 cut(s) 455, 1258, 2177, 2361
BciVI GTATCC 3 cut(s) 154, 562, 2327
BclI TGATCA 1 cut(s) 1761
BcnI CCSGG 1 cut(s) 1538
BcoDI GTCTC 1 cut(s) 1386
BcuI ACTAGT 1 cut(s) 1556
BfaI CTAG 6 cut(s) 198, 468, 512, 1557, 1880, 2714
BfmI CTRYAG 3 cut(s) 381, 1847, 2327
BfrI CTTAAG 1 cut(s) 1385
BfuI GTATCC 3 cut(s) 154, 562, 2327
BglII AGATCT 1 cut(s) 1909
BisI GCNGC 2 cut(s) 2571, 2582
BlsI GCNGC 2 cut(s) 2572, 2583
BmcAI AGTACT 1 cut(s) 1984
Bme1390I CCNGG 5 cut(s) 455, 1258, 1538, 2177, 2361
Bme18I GGWCC 3 cut(s) 1852, 2357, 2535
BmgT120I GGNCC 5 cut(s) 848, 1852, 1944, 2357, 2535
BmiI GGNNCC 6 cut(s) 1297, 1534, 2358, 2536, 2537, 2637
BmrFI CCNGG 5 cut(s) 455, 1258, 1538, 2177, 2361
BmsI GCATC 7 cut(s) 367, 505, 634, 994, 1328, 1923, 2705
BpmI CTGGAG 4 cut(s) 463, 776, 807, 1282
Bpu10I CCTNAGC 1 cut(s) 1611
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 5 cut(s) 1924, 2378, 2495, 2512, 2525
BpuMI CCSGG 1 cut(s) 1538
Bsa29I ATCGAT 2 cut(s) 2463, 2739
BsaAI YACGTR 2 cut(s) 1355, 2011
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 4 cut(s) 1256, 1371, 1536, 2320
BsaWI WCCGGW 1 cut(s) 2798
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 441, 460, 841, 1262, 2129, 2777
Bse1I ACTGG 4 cut(s) 733, 1124, 1421, 1707
Bse3DI GCAATG 2 cut(s) 1136, 2452
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 4 cut(s) 455, 1258, 2177, 2361
BseCI ATCGAT 2 cut(s) 2463, 2739
BseDI CCNNGG 4 cut(s) 1256, 1371, 1536, 2320
BseGI GGATG 3 cut(s) 929, 1365, 2063
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 7 cut(s) 217, 441, 460, 841, 1262, 2129, 2777
BseMI GCAATG 2 cut(s) 1136, 2452
BseMII CTCAG 4 cut(s) 149, 1602, 1983, 2567
BseNI ACTGG 4 cut(s) 733, 1124, 1421, 1707
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2638
BseXI GCAGC 2 cut(s) 2582, 2593
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 1991
BshFI GGCC 7 cut(s) 814, 850, 879, 1011, 1864, 1946, 2133
BshNI GGYRCC 1 cut(s) 2635
BshVI ATCGAT 2 cut(s) 2463, 2739
BsiSI CCGG 2 cut(s) 1538, 2799
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1503, 2548
BslI CCNNNNNNNGG 7 cut(s) 217, 441, 460, 841, 1262, 2129, 2777
BsmAI GTCTC 1 cut(s) 1386
BsmFI GGGAC 3 cut(s) 761, 1503, 2548
BsnI GGCC 7 cut(s) 814, 850, 879, 1011, 1864, 1946, 2133
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 1257, 1298, 2638
BspACI CCGC 2 cut(s) 940, 1642
BspANI GGCC 7 cut(s) 814, 850, 879, 1011, 1864, 1946, 2133
BspCNI CTCAG 4 cut(s) 148, 1603, 1984, 2568
BspDI ATCGAT 2 cut(s) 2463, 2739
BspLI GGNNCC 6 cut(s) 1297, 1534, 2358, 2536, 2537, 2637
BspMAI CTGCAG 1 cut(s) 2331
BspPI GGATC 5 cut(s) 68, 95, 901, 1996, 2703
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2635
BspTI CTTAAG 1 cut(s) 1385
BsrDI GCAATG 2 cut(s) 1136, 2452
BsrI ACTGG 4 cut(s) 733, 1124, 1421, 1707
BssECI CCNNGG 4 cut(s) 1256, 1371, 1536, 2320
BssNAI GTATAC 1 cut(s) 534
Bst1107I GTATAC 1 cut(s) 534
Bst2UI CCWGG 4 cut(s) 455, 1258, 2177, 2361
Bst4CI ACNGT 7 cut(s) 775, 1626, 1711, 1774, 1809, 2350, 2731
Bst6I CTCTTC 3 cut(s) 12, 250, 318
BstAFI CTTAAG 1 cut(s) 1385
BstAPI GCANNNNNTGC 1 cut(s) 2567
BstBAI YACGTR 2 cut(s) 1355, 2011
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 5 cut(s) 942, 1009, 1040, 1729, 1821
BstDEI CTNAG 7 cut(s) 135, 1611, 1917, 1951, 1992, 2258, 2576
BstDSI CCRYGG 2 cut(s) 1371, 2320
BstEII GGTNACC 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 2127
BstF5I GGATG 3 cut(s) 929, 1365, 2063
BstMAI GTCTC 1 cut(s) 1386
BstMWI GCNNNNNNNGC 5 cut(s) 1183, 1607, 1616, 2479, 2567
BstNI CCWGG 4 cut(s) 455, 1258, 2177, 2361
BstNSI RCATGY 3 cut(s) 859, 1024, 1249
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 5 cut(s) 453, 1256, 1536, 2175, 2359
BstSFI CTRYAG 3 cut(s) 381, 1847, 2327
BstSLI GKGCMC 1 cut(s) 2638
BstV1I GCAGC 2 cut(s) 2582, 2593
BstX2I RGATCY 3 cut(s) 893, 1909, 2708
BstXI CCANNNNNNTGG 1 cut(s) 1529
BstYI RGATCY 3 cut(s) 893, 1909, 2708
BstZ17I GTATAC 1 cut(s) 534
Bsu15I ATCGAT 2 cut(s) 2463, 2739
BsuI GTATCC 3 cut(s) 154, 562, 2327
BsuRI GGCC 7 cut(s) 814, 850, 879, 1011, 1864, 1946, 2133
BsuTUI ATCGAT 2 cut(s) 2463, 2739
BtgI CCRYGG 2 cut(s) 1371, 2320
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 3 cut(s) 929, 1365, 2063
BtsIMutI CAGTG 3 cut(s) 162, 2355, 2573
Cac8I GCNNGC 5 cut(s) 942, 1009, 1040, 1729, 1821
Cfr13I GGNCC 5 cut(s) 848, 1852, 1944, 2357, 2535
ClaI ATCGAT 2 cut(s) 2463, 2739
Csp6I GTAC 9 cut(s) 36, 52, 208, 235, 1459, 1627, 1786, 1983, 2660
CviQI GTAC 9 cut(s) 36, 52, 208, 235, 1459, 1627, 1786, 1983, 2660
DdeI CTNAG 7 cut(s) 135, 1611, 1917, 1951, 1992, 2258, 2576
DraI TTTAAA 1 cut(s) 1735
DraIII CACNNNGTG 1 cut(s) 1652
EaeI YGGCCR 2 cut(s) 877, 1862
Eam1104I CTCTTC 3 cut(s) 12, 250, 318
EarI CTCTTC 3 cut(s) 12, 250, 318
Eco147I AGGCCT 1 cut(s) 2133
Eco24I GRGCYC 2 cut(s) 1257, 1298
Eco47I GGWCC 3 cut(s) 1852, 2357, 2535
Eco57I CTGAAG 3 cut(s) 1135, 2007, 2198
Eco91I GGTNACC 1 cut(s) 866
EcoNI CCTNNNNNAGG 1 cut(s) 2127
EcoO109I RGGNCCY 2 cut(s) 1944, 2535
EcoO65I GGTNACC 1 cut(s) 866
EcoRI GAATTC 3 cut(s) 1302, 2152, 2435
EcoRII CCWGG 4 cut(s) 453, 1256, 2175, 2359
EcoT22I ATGCAT 2 cut(s) 627, 2698
EcoT38I GRGCYC 2 cut(s) 1257, 1298
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 3 cut(s) 761, 1503, 2548
FauI CCCGC 1 cut(s) 933
FauNDI CATATG 2 cut(s) 183, 1444
FbaI TGATCA 1 cut(s) 1761
FblI GTMKAC 1 cut(s) 533
Fnu4HI GCNGC 2 cut(s) 2571, 2582
FokI GGATG 3 cut(s) 936, 1352, 2050
FriOI GRGCYC 2 cut(s) 1257, 1298
Fsp4HI GCNGC 2 cut(s) 2571, 2582
FspBI CTAG 6 cut(s) 198, 468, 512, 1557, 1880, 2714
GluI GCNGC 2 cut(s) 2571, 2582
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 4 cut(s) 463, 776, 807, 1282
HaeIII GGCC 7 cut(s) 814, 850, 879, 1011, 1864, 1946, 2133
HapII CCGG 2 cut(s) 1538, 2799
HindIII AAGCTT 5 cut(s) 1184, 1729, 2093, 2411, 2471
HinfI GANTC 7 cut(s) 760, 950, 1206, 1217, 1994, 2085, 2674
HpaII CCGG 2 cut(s) 1538, 2799
HphI GGTGA 9 cut(s) 743, 824, 860, 959, 991, 1247, 1431, 1675, 2243
Hpy166II GTNNAC 4 cut(s) 534, 2167, 2324, 2660
Hpy188I TCNGA 9 cut(s) 138, 420, 436, 748, 770, 1205, 2530, 2701, 2759
Hpy8I GTNNAC 4 cut(s) 534, 2167, 2324, 2660
HpyCH4III ACNGT 7 cut(s) 775, 1626, 1711, 1774, 1809, 2350, 2731
HpyCH4IV ACGT 7 cut(s) 164, 1053, 1354, 1497, 2010, 2365, 2399
HpyF10VI GCNNNNNNNGC 5 cut(s) 1183, 1607, 1616, 2479, 2567
HpyF3I CTNAG 7 cut(s) 135, 1611, 1917, 1951, 1992, 2258, 2576
HpySE526I ACGT 7 cut(s) 164, 1053, 1354, 1497, 2010, 2365, 2399
KflI GGGWCCC 1 cut(s) 2535
Ksp22I TGATCA 1 cut(s) 1761
LmnI GCTCC 4 cut(s) 178, 1301, 1538, 2801
Lsp1109I GCAGC 2 cut(s) 2582, 2593
LweI GCATC 7 cut(s) 367, 505, 634, 994, 1328, 1923, 2705
MaeI CTAG 6 cut(s) 198, 468, 512, 1557, 1880, 2714
MaeII ACGT 7 cut(s) 164, 1053, 1354, 1497, 2010, 2365, 2399
MaeIII GTNAC 8 cut(s) 866, 1062, 1437, 1663, 1774, 1958, 2279, 2763
MfeI CAATTG 1 cut(s) 1513
MflI RGATCY 3 cut(s) 893, 1909, 2708
MhlI GDGCHC 3 cut(s) 1257, 1298, 2638
MlsI TGGCCA 1 cut(s) 1864
MluNI TGGCCA 1 cut(s) 1864
MlyI GAGTC 3 cut(s) 1226, 2003, 2094
Mox20I TGGCCA 1 cut(s) 1864
Mph1103I ATGCAT 2 cut(s) 627, 2698
MscI TGGCCA 1 cut(s) 1864
MslI CAYNNNNRTG 4 cut(s) 495, 922, 1527, 2187
Msp20I TGGCCA 1 cut(s) 1864
MspA1I CMGCKG 1 cut(s) 374
MspCI CTTAAG 1 cut(s) 1385
MspI CCGG 2 cut(s) 1538, 2799
MspR9I CCNGG 5 cut(s) 455, 1258, 1538, 2177, 2361
MunI CAATTG 1 cut(s) 1513
MvaI CCWGG 4 cut(s) 455, 1258, 2177, 2361
MwoI GCNNNNNNNGC 5 cut(s) 1183, 1607, 1616, 2479, 2567
NciI CCSGG 1 cut(s) 1538
NdeI CATATG 2 cut(s) 183, 1444
NlaIV GGNNCC 6 cut(s) 1297, 1534, 2358, 2536, 2537, 2637
NmuCI GTSAC 5 cut(s) 866, 1437, 1663, 1774, 2763
NsiI ATGCAT 2 cut(s) 627, 2698
NspI RCATGY 3 cut(s) 859, 1024, 1249
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 1 cut(s) 2133
PciI ACATGT 1 cut(s) 1245
PfeI GAWTC 4 cut(s) 760, 950, 1206, 2674
Pfl23II CGTACG 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 2359
PkrI GCNGC 2 cut(s) 2572, 2583
PleI GAGTC 3 cut(s) 1225, 2002, 2093
PpsI GAGTC 3 cut(s) 1225, 2002, 2093
Ppu21I YACGTR 2 cut(s) 1355, 2011
PpuMI RGGWCCY 1 cut(s) 2535
PscI ACATGT 1 cut(s) 1245
PshBI ATTAAT 1 cut(s) 306
PsiI TTATAA 1 cut(s) 1680
Psp5II RGGWCCY 1 cut(s) 2535
Psp6I CCWGG 4 cut(s) 453, 1256, 2175, 2359
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 4 cut(s) 453, 1256, 2175, 2359
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1297, 1534, 2358, 2536, 2537, 2637
PspPI GGNCC 5 cut(s) 848, 1852, 1944, 2357, 2535
PspPPI RGGWCCY 1 cut(s) 2535
PstI CTGCAG 1 cut(s) 2331
PsuI RGATCY 3 cut(s) 893, 1909, 2708
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 9 cut(s) 37, 53, 209, 236, 1460, 1628, 1787, 1984, 2661
RsaNI GTAC 9 cut(s) 36, 52, 208, 235, 1459, 1627, 1786, 1983, 2660
RseI CAYNNNNRTG 4 cut(s) 495, 922, 1527, 2187
SatI GCNGC 2 cut(s) 2571, 2582
Sau96I GGNCC 5 cut(s) 848, 1852, 1944, 2357, 2535
ScaI AGTACT 1 cut(s) 1984
SchI GAGTC 3 cut(s) 1226, 2003, 2094
ScrFI CCNGG 5 cut(s) 455, 1258, 1538, 2177, 2361
SduI GDGCHC 3 cut(s) 1257, 1298, 2638
SfaNI GCATC 7 cut(s) 367, 505, 634, 994, 1328, 1923, 2705
SfcI CTRYAG 3 cut(s) 381, 1847, 2327
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 3 cut(s) 1852, 2357, 2535
SmiMI CAYNNNNRTG 4 cut(s) 495, 922, 1527, 2187
SmlI CTYRAG 6 cut(s) 1385, 1939, 2393, 2474, 2491, 2540
SmoI CTYRAG 6 cut(s) 1385, 1939, 2393, 2474, 2491, 2540
SpeI ACTAGT 1 cut(s) 1556
SseBI AGGCCT 1 cut(s) 2133
SsiI CCGC 2 cut(s) 940, 1642
SspI AATATT 1 cut(s) 703
SspMI CTAG 6 cut(s) 198, 468, 512, 1557, 1880, 2714
StuI AGGCCT 1 cut(s) 2133
StyD4I CCNGG 5 cut(s) 453, 1256, 1536, 2175, 2359
TaaI ACNGT 7 cut(s) 775, 1626, 1711, 1774, 1809, 2350, 2731
TaiI ACGT 7 cut(s) 167, 1056, 1357, 1500, 2013, 2368, 2402
TaqI TCGA 7 cut(s) 243, 300, 547, 697, 2185, 2463, 2739
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 4 cut(s) 51, 234, 1626, 1982
TfiI GAWTC 4 cut(s) 760, 950, 1206, 2674
TscAI CASTG 3 cut(s) 162, 2355, 2580
TseFI GTSAC 5 cut(s) 866, 1437, 1663, 1774, 2763
TseI GCWGC 2 cut(s) 2570, 2581
Tsp45I GTSAC 5 cut(s) 866, 1437, 1663, 1774, 2763
TspGWI ACGGA 4 cut(s) 53, 654, 1388, 2309
TspRI CASTG 3 cut(s) 162, 2355, 2580
Van91I CCANNNNNTGG 1 cut(s) 460
Vha464I CTTAAG 1 cut(s) 1385
VpaK11BI GGWCC 3 cut(s) 1852, 2357, 2535
VspI ATTAAT 1 cut(s) 306
XagI CCTNNNNNAGG 1 cut(s) 2127
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 3 cut(s) 859, 1024, 1249
XmiI GTMKAC 1 cut(s) 533
XspI CTAG 6 cut(s) 198, 468, 512, 1557, 1880, 2714
ZrmI AGTACT 1 cut(s) 1984
Zsp2I ATGCAT 2 cut(s) 627, 2698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.