Rh3DG274100

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001584
Physical Location & Seq
Forward (+)
27706731 .. 27721292
14562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG274100.1

Sequence Viewer

Length: 2892 bp
ATGGCTGGGGTGAAAGAAGAGATATTGAAAGCCCGTACGGACAACCGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCGGTGTTGGTTCTTTCAGCGACCCTGATGGCGTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGACAGCTACTTGAAGTACATCATCGAGCATGGAGGAAGAGGAAATGCTTTCACTTCTGAAGAACACACCAGCTATCGTTTCGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGCGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAACTCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATCGGTATACTTTGTGGGTTCGACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTAAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCGTGCATGTATCTTGGTCACCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGCGGGCTGGTGAATCATTTTCTACTTTGGACTTTTCTTTCTTTGAAGTAGATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACGTTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTATTTGTGAGACAAAGTTTCATTATCAGGACAAAATTGAGCCTCTTAATTACGTGATCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATTGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAAAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGTGAATTTACACCTACCAGCACGTAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCAGCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTCATTTGTTAATGGATTACTTGAATGATTATGCTTATTATGCTGAGGTTGCTGGACTGTACTGTGGAATATACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAACGGTCACAAAGGAGTACCAAAATTACAAATTTGAACAGTCCTATGAGCAGGCTATTTACTATTGCTCATTAGTTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTACTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATGTTTTTTTTAAGGGTTCAAACCGTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGGAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTATTTGTTCTTATTGCAAAGCAACCAGCCTTTCACCAACTTAGAATAGTTGAGCAACTTGGTTACATAACTCGTCTTTCACAGAGGAATGATTGTGGTATCCGTGGACTGCAGTTCATTATACAATCCACAGTGAAGGGTCCAGGACGTATTGATTTGAGAGTGGAGGAATTTCTCAAGACGTTTGAGAGTAAGCTTTATGAGATGACAAATAACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACCAGAACTTGAGGGAAGAAGCTGAATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTTCAATGAGCATATAAAAGTTGGGGCACCTCATAAGAGGACATTGAGTGTACGAGTATATGGGAATCGTCATTCATTTGAGTATGCATCAGATAAAAGATCCCTAGTGCAACCTTGCAACGTCAAAATCGATGATATTTTCGCTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAGCTCCGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

963

Amino Acids

111.21

Weight (kDa)

6.46

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 5.6e-34 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 2.3e-28 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 653 2.1e-100 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 658 - 844 2e-10 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 769 - 864 1.1e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1755
AccB1I GGYRCC 1 cut(s) 2710
AccI GTMKAC 1 cut(s) 533
AciI CCGC 1 cut(s) 940
AclWI GGATC 5 cut(s) 68, 95, 901, 2071, 2778
AcoI YGGCCR 2 cut(s) 877, 1937
AcuI CTGAAG 5 cut(s) 312, 458, 1222, 2082, 2273
AfiI CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2204, 2852
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AhlI ACTAGT 1 cut(s) 1631
AjnI CCWGG 3 cut(s) 1343, 2250, 2434
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw26I GTCTC 2 cut(s) 1112, 1473
AlwI GGATC 5 cut(s) 68, 95, 901, 2071, 2778
AoxI GGCC 9 cut(s) 175, 667, 812, 848, 877, 1009, 1937, 2019, 2206
ApeKI GCWGC 4 cut(s) 122, 491, 2645, 2656
AseI ATTAAT 1 cut(s) 321
AspS9I GGNCC 6 cut(s) 175, 848, 1927, 2019, 2432, 2610
AsuC2I CCSGG 2 cut(s) 47, 1613
AsuHPI GGTGA 9 cut(s) 22, 743, 824, 860, 959, 991, 1334, 1750, 2318
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1927, 2432, 2610
BaeGI GKGCMC 1 cut(s) 2713
BalI TGGCCA 1 cut(s) 1939
BanI GGYRCC 1 cut(s) 2710
BanII GRGCYC 2 cut(s) 1344, 1385
BbvCI CCTCAGC 1 cut(s) 1686
BbvI GCAGC 4 cut(s) 109, 503, 2657, 2668
BccI CCATC 7 cut(s) 158, 323, 446, 918, 1939, 2146, 2600
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 3 cut(s) 1345, 2252, 2436
BciVI GTATCC 2 cut(s) 562, 2402
BclI TGATCA 2 cut(s) 1164, 1836
BcnI CCSGG 2 cut(s) 47, 1613
BcoDI GTCTC 2 cut(s) 1112, 1473
BcuI ACTAGT 1 cut(s) 1631
BfaI CTAG 4 cut(s) 213, 1632, 1955, 2789
BfmI CTRYAG 3 cut(s) 396, 1922, 2402
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 2 cut(s) 562, 2402
BglII AGATCT 1 cut(s) 1984
BisI GCNGC 4 cut(s) 123, 492, 2646, 2657
BlsI GCNGC 4 cut(s) 124, 493, 2647, 2658
BmcAI AGTACT 1 cut(s) 2059
Bme1390I CCNGG 5 cut(s) 47, 1345, 1613, 2252, 2436
Bme18I GGWCC 3 cut(s) 1927, 2432, 2610
BmgT120I GGNCC 6 cut(s) 175, 848, 1927, 2019, 2432, 2610
BmiI GGNNCC 6 cut(s) 1384, 1609, 2433, 2611, 2612, 2712
BmrFI CCNGG 5 cut(s) 47, 1345, 1613, 2252, 2436
BmsI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 1998, 2780
BpmI CTGGAG 4 cut(s) 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1686
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 6 cut(s) 212, 1999, 2453, 2570, 2587, 2600
BpuMI CCSGG 2 cut(s) 47, 1613
Bsa29I ATCGAT 2 cut(s) 2538, 2814
BsaAI YACGTR 3 cut(s) 1162, 1442, 2086
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 5 cut(s) 129, 1343, 1458, 1611, 2395
BsaWI WCCGGW 1 cut(s) 2873
Bsc4I CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2204, 2852
Bse1I ACTGG 4 cut(s) 733, 1211, 1496, 1782
Bse3DI GCAATG 2 cut(s) 1223, 2527
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 3 cut(s) 1345, 2252, 2436
BseCI ATCGAT 2 cut(s) 2538, 2814
BseDI CCNNGG 5 cut(s) 129, 1343, 1458, 1611, 2395
BseGI GGATG 5 cut(s) 139, 499, 929, 1452, 2138
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2204, 2852
BseMI GCAATG 2 cut(s) 1223, 2527
BseMII CTCAG 4 cut(s) 374, 1677, 2058, 2642
BseNI ACTGG 4 cut(s) 733, 1211, 1496, 1782
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2713
BseXI GCAGC 4 cut(s) 109, 503, 2657, 2668
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 2 cut(s) 510, 2066
BshFI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1939, 2021, 2208
BshNI GGYRCC 1 cut(s) 2710
BshVI ATCGAT 2 cut(s) 2538, 2814
BsiSI CCGG 3 cut(s) 46, 1613, 2874
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1578, 2623
BslI CCNNNNNNNGG 7 cut(s) 232, 456, 676, 841, 1349, 2204, 2852
BsmAI GTCTC 2 cut(s) 1112, 1473
BsmFI GGGAC 3 cut(s) 761, 1578, 2623
BsnI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1939, 2021, 2208
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 1344, 1385, 2713
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 940
BspANI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1939, 2021, 2208
BspCNI CTCAG 4 cut(s) 373, 1678, 2059, 2643
BspDI ATCGAT 2 cut(s) 2538, 2814
BspLI GGNNCC 6 cut(s) 1384, 1609, 2433, 2611, 2612, 2712
BspMAI CTGCAG 1 cut(s) 2406
BspPI GGATC 5 cut(s) 68, 95, 901, 2071, 2778
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2710
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 2 cut(s) 1223, 2527
BsrI ACTGG 4 cut(s) 733, 1211, 1496, 1782
BssECI CCNNGG 5 cut(s) 129, 1343, 1458, 1611, 2395
BssNAI GTATAC 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 129
Bst1107I GTATAC 1 cut(s) 534
Bst2UI CCWGG 3 cut(s) 1345, 2252, 2436
Bst4CI ACNGT 8 cut(s) 775, 1701, 1706, 1786, 1849, 1884, 2117, 2425
Bst6I CTCTTC 3 cut(s) 12, 265, 333
BstAFI CTTAAG 1 cut(s) 1472
BstAPI GCANNNNNTGC 1 cut(s) 2642
BstBAI YACGTR 3 cut(s) 1162, 1442, 2086
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 5 cut(s) 942, 1009, 1040, 1804, 1896
BstDEI CTNAG 8 cut(s) 360, 485, 1686, 1992, 2026, 2067, 2333, 2651
BstDSI CCRYGG 3 cut(s) 129, 1458, 2395
BstEII GGTNACC 1 cut(s) 866
BstENI CCTNNNNNAGG 2 cut(s) 674, 2202
BstF5I GGATG 5 cut(s) 139, 499, 929, 1452, 2138
BstMAI GTCTC 2 cut(s) 1112, 1473
BstMWI GCNNNNNNNGC 7 cut(s) 174, 478, 1270, 1682, 1691, 2554, 2642
BstNI CCWGG 3 cut(s) 1345, 2252, 2436
BstNSI RCATGY 3 cut(s) 859, 1024, 1336
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 5 cut(s) 45, 1343, 1611, 2250, 2434
BstSFI CTRYAG 3 cut(s) 396, 1922, 2402
BstSLI GKGCMC 1 cut(s) 2713
BstV1I GCAGC 4 cut(s) 109, 503, 2657, 2668
BstX2I RGATCY 3 cut(s) 893, 1984, 2783
BstXI CCANNNNNNTGG 1 cut(s) 1604
BstYI RGATCY 3 cut(s) 893, 1984, 2783
BstZ17I GTATAC 1 cut(s) 534
Bsu15I ATCGAT 2 cut(s) 2538, 2814
BsuI GTATCC 2 cut(s) 562, 2402
BsuRI GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1939, 2021, 2208
BsuTUI ATCGAT 2 cut(s) 2538, 2814
BtgI CCRYGG 3 cut(s) 129, 1458, 2395
BtsCI GGATG 5 cut(s) 139, 499, 929, 1452, 2138
BtsIMutI CAGTG 2 cut(s) 2430, 2648
Cac8I GCNNGC 5 cut(s) 942, 1009, 1040, 1804, 1896
Cfr13I GGNCC 6 cut(s) 175, 848, 1927, 2019, 2432, 2610
ClaI ATCGAT 2 cut(s) 2538, 2814
DdeI CTNAG 8 cut(s) 360, 485, 1686, 1992, 2026, 2067, 2333, 2651
DraI TTTAAA 1 cut(s) 1810
EaeI YGGCCR 2 cut(s) 877, 1937
Eam1104I CTCTTC 3 cut(s) 12, 265, 333
EarI CTCTTC 3 cut(s) 12, 265, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 669, 2208
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco47I GGWCC 3 cut(s) 1927, 2432, 2610
Eco57I CTGAAG 5 cut(s) 312, 458, 1222, 2082, 2273
Eco91I GGTNACC 1 cut(s) 866
EcoNI CCTNNNNNAGG 2 cut(s) 674, 2202
EcoO109I RGGNCCY 3 cut(s) 175, 2019, 2610
EcoO65I GGTNACC 1 cut(s) 866
EcoRI GAATTC 3 cut(s) 1389, 2227, 2510
EcoRII CCWGG 3 cut(s) 1343, 2250, 2434
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 627, 2773
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 3 cut(s) 761, 1578, 2623
FauI CCCGC 1 cut(s) 933
FauNDI CATATG 2 cut(s) 198, 1519
FbaI TGATCA 2 cut(s) 1164, 1836
FblI GTMKAC 1 cut(s) 533
Fnu4HI GCNGC 4 cut(s) 123, 492, 2646, 2657
FokI GGATG 5 cut(s) 146, 486, 936, 1439, 2125
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 4 cut(s) 123, 492, 2646, 2657
FspBI CTAG 4 cut(s) 213, 1632, 1955, 2789
GluI GCNGC 4 cut(s) 123, 492, 2646, 2657
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 4 cut(s) 478, 776, 807, 1369
HaeIII GGCC 9 cut(s) 177, 669, 814, 850, 879, 1011, 1939, 2021, 2208
HapII CCGG 3 cut(s) 46, 1613, 2874
HindIII AAGCTT 5 cut(s) 1271, 1804, 2168, 2486, 2546
HinfI GANTC 8 cut(s) 496, 760, 950, 1293, 1304, 2069, 2160, 2749
HpaII CCGG 3 cut(s) 46, 1613, 2874
HphI GGTGA 9 cut(s) 22, 743, 824, 860, 959, 991, 1334, 1750, 2318
Hpy166II GTNNAC 4 cut(s) 534, 2242, 2399, 2735
Hpy8I GTNNAC 4 cut(s) 534, 2242, 2399, 2735
HpyCH4III ACNGT 8 cut(s) 775, 1701, 1706, 1786, 1849, 1884, 2117, 2425
HpyCH4IV ACGT 8 cut(s) 1053, 1161, 1441, 1572, 2085, 2440, 2474, 2805
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 478, 1270, 1682, 1691, 2554, 2642
HpyF3I CTNAG 8 cut(s) 360, 485, 1686, 1992, 2026, 2067, 2333, 2651
HpySE526I ACGT 8 cut(s) 1053, 1161, 1441, 1572, 2085, 2440, 2474, 2805
KflI GGGWCCC 1 cut(s) 2610
Ksp22I TGATCA 2 cut(s) 1164, 1836
LmnI GCTCC 3 cut(s) 1388, 1613, 2876
Lsp1109I GCAGC 4 cut(s) 109, 503, 2657, 2668
LweI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 1998, 2780
MaeI CTAG 4 cut(s) 213, 1632, 1955, 2789
MaeII ACGT 8 cut(s) 1053, 1161, 1441, 1572, 2085, 2440, 2474, 2805
MaeIII GTNAC 7 cut(s) 866, 1062, 1738, 1849, 2033, 2354, 2838
MfeI CAATTG 1 cut(s) 1588
MflI RGATCY 3 cut(s) 893, 1984, 2783
MhlI GDGCHC 3 cut(s) 1344, 1385, 2713
MlsI TGGCCA 1 cut(s) 1939
MluNI TGGCCA 1 cut(s) 1939
MlyI GAGTC 3 cut(s) 1313, 2078, 2169
Mox20I TGGCCA 1 cut(s) 1939
Mph1103I ATGCAT 2 cut(s) 627, 2773
MscI TGGCCA 1 cut(s) 1939
MslI CAYNNNNRTG 4 cut(s) 486, 922, 1602, 2262
Msp20I TGGCCA 1 cut(s) 1939
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1472
MspI CCGG 3 cut(s) 46, 1613, 2874
MspR9I CCNGG 5 cut(s) 47, 1345, 1613, 2252, 2436
MunI CAATTG 1 cut(s) 1588
MvaI CCWGG 3 cut(s) 1345, 2252, 2436
MwoI GCNNNNNNNGC 7 cut(s) 174, 478, 1270, 1682, 1691, 2554, 2642
NciI CCSGG 2 cut(s) 47, 1613
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 198, 1519
NlaIV GGNNCC 6 cut(s) 1384, 1609, 2433, 2611, 2612, 2712
NmuCI GTSAC 4 cut(s) 866, 1738, 1849, 2838
NsiI ATGCAT 2 cut(s) 627, 2773
NspI RCATGY 3 cut(s) 859, 1024, 1336
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 2 cut(s) 669, 2208
PciI ACATGT 1 cut(s) 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 2811
PfeI GAWTC 5 cut(s) 496, 760, 950, 1293, 2749
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2434
PkrI GCNGC 4 cut(s) 124, 493, 2647, 2658
PleI GAGTC 3 cut(s) 1312, 2077, 2168
PpsI GAGTC 3 cut(s) 1312, 2077, 2168
Ppu21I YACGTR 3 cut(s) 1162, 1442, 2086
PpuMI RGGWCCY 1 cut(s) 2610
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 1 cut(s) 321
PsiI TTATAA 1 cut(s) 1755
Psp5II RGGWCCY 1 cut(s) 2610
Psp6I CCWGG 3 cut(s) 1343, 2250, 2434
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 1343, 2250, 2434
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1384, 1609, 2433, 2611, 2612, 2712
PspPI GGNCC 6 cut(s) 175, 848, 1927, 2019, 2432, 2610
PspPPI RGGWCCY 1 cut(s) 2610
PstI CTGCAG 1 cut(s) 2406
PsuI RGATCY 3 cut(s) 893, 1984, 2783
PvuII CAGCTG 1 cut(s) 389
RseI CAYNNNNRTG 4 cut(s) 486, 922, 1602, 2262
SatI GCNGC 4 cut(s) 123, 492, 2646, 2657
Sau96I GGNCC 6 cut(s) 175, 848, 1927, 2019, 2432, 2610
ScaI AGTACT 1 cut(s) 2059
SchI GAGTC 3 cut(s) 1313, 2078, 2169
ScrFI CCNGG 5 cut(s) 47, 1345, 1613, 2252, 2436
SduI GDGCHC 3 cut(s) 1344, 1385, 2713
SfaNI GCATC 7 cut(s) 382, 490, 634, 994, 1415, 1998, 2780
SfcI CTRYAG 3 cut(s) 396, 1922, 2402
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 3 cut(s) 1927, 2432, 2610
SmiMI CAYNNNNRTG 4 cut(s) 486, 922, 1602, 2262
SmlI CTYRAG 7 cut(s) 191, 1472, 2014, 2468, 2549, 2566, 2615
SmoI CTYRAG 7 cut(s) 191, 1472, 2014, 2468, 2549, 2566, 2615
SpeI ACTAGT 1 cut(s) 1631
SseBI AGGCCT 2 cut(s) 669, 2208
SsiI CCGC 1 cut(s) 940
SspI AATATT 2 cut(s) 703, 1171
SspMI CTAG 4 cut(s) 213, 1632, 1955, 2789
StuI AGGCCT 2 cut(s) 669, 2208
StyD4I CCNGG 5 cut(s) 45, 1343, 1611, 2250, 2434
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 8 cut(s) 775, 1701, 1706, 1786, 1849, 1884, 2117, 2425
TaiI ACGT 8 cut(s) 1056, 1164, 1444, 1575, 2088, 2443, 2477, 2808
TaqI TCGA 7 cut(s) 258, 315, 547, 697, 2260, 2538, 2814
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 5 cut(s) 51, 249, 518, 1701, 2057
TfiI GAWTC 5 cut(s) 496, 760, 950, 1293, 2749
TscAI CASTG 2 cut(s) 2430, 2655
TseFI GTSAC 4 cut(s) 866, 1738, 1849, 2838
TseI GCWGC 4 cut(s) 122, 491, 2645, 2656
Tsp45I GTSAC 4 cut(s) 866, 1738, 1849, 2838
TspGWI ACGGA 4 cut(s) 53, 654, 1475, 2384
TspRI CASTG 2 cut(s) 2430, 2655
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1927, 2432, 2610
VspI ATTAAT 1 cut(s) 321
XagI CCTNNNNNAGG 2 cut(s) 674, 2202
XceI RCATGY 3 cut(s) 859, 1024, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1724
XmiI GTMKAC 1 cut(s) 533
XspI CTAG 4 cut(s) 213, 1632, 1955, 2789
ZrmI AGTACT 1 cut(s) 2059
Zsp2I ATGCAT 2 cut(s) 627, 2773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.