Rh3DG273700

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001584
Physical Location & Seq
Forward (+)
27557454 .. 27572227
14774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG273700.1

Sequence Viewer

Length: 2544 bp
ATGGCTGTGGGGAAAGGAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTTCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATAGTTACTCCAAGTACATCACTGAGCATGGAGGAAGAACAAATGCTTTCACAGCTGCAGAACACACCAACTTTTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCTGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTTGTACAGGCAATTGGGATACTTTGGAGGTTCGACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGAGCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGCGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCCATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATGCAAACATGTTCGAGCCCTGGTATGAAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATAAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCAACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATACAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCCAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGGAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTCAGGTTGCTGAACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTCTTGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGGAGTACCAAAATTACAAATTTCAACAGCCTTATGAGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCAATGATGCTCTCAAGGGCCTTCTTAGAGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATATTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAGGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATGAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTGCTTGTTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTATGTAACTGCTCTTTTACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGGTGAGGTCAACCAGGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

847

Amino Acids

97.72

Weight (kDa)

5.89

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 9.9e-37 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 1e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 655 1e-102 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 660 - 841 1.9e-10 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 768 - 842 4.4e-15 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1761
AccII CGCG 1 cut(s) 2403
AciI CCGC 2 cut(s) 2401, 2403
AclI AACGTT 1 cut(s) 2480
AclWI GGATC 4 cut(s) 68, 95, 901, 2077
AcoI YGGCCR 2 cut(s) 877, 1943
AcuI CTGAAG 5 cut(s) 458, 1222, 1641, 2088, 2279
AfiI CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AjnI CCWGG 5 cut(s) 168, 1343, 2256, 2440, 2534
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 3 cut(s) 796, 1112, 1473
AlwI GGATC 4 cut(s) 68, 95, 901, 2077
ApeKI GCWGC 4 cut(s) 122, 290, 491, 2299
ArsI GACNNNNNNTTYG 2 cut(s) 1563, 1595
AseI ATTAAT 3 cut(s) 321, 1155, 1922
Asp700I GAANNNNTTC 2 cut(s) 87, 1968
AspS9I GGNCC 6 cut(s) 175, 848, 1165, 1933, 2025, 2438
AsuHPI GGTGA 8 cut(s) 743, 824, 959, 991, 1334, 1512, 1756, 2536
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1165, 1933, 2438
BalI TGGCCA 1 cut(s) 1945
BanII GRGCYC 2 cut(s) 1344, 1385
BauI CACGAG 1 cut(s) 1013
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 4 cut(s) 109, 277, 503, 2286
BccI CCATC 6 cut(s) 158, 323, 446, 918, 1945, 2152
BciT130I CCWGG 5 cut(s) 170, 1345, 2258, 2442, 2536
BciVI GTATCC 3 cut(s) 526, 562, 2408
BclI TGATCA 1 cut(s) 1842
BcoDI GTCTC 3 cut(s) 796, 1112, 1473
BfaI CTAG 3 cut(s) 213, 1961, 2542
BfmI CTRYAG 3 cut(s) 291, 1928, 2408
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 3 cut(s) 526, 562, 2408
BglI GCCNNNNNGGC 1 cut(s) 174
BglII AGATCT 1 cut(s) 1990
BisI GCNGC 4 cut(s) 123, 291, 492, 2300
BlsI GCNGC 4 cut(s) 124, 292, 493, 2301
BmcAI AGTACT 1 cut(s) 2065
Bme1390I CCNGG 5 cut(s) 170, 1345, 2258, 2442, 2536
Bme18I GGWCC 3 cut(s) 1165, 1933, 2438
BmgT120I GGNCC 6 cut(s) 175, 848, 1165, 1933, 2025, 2438
BmiI GGNNCC 3 cut(s) 1384, 1615, 2439
BmrFI CCNGG 5 cut(s) 170, 1345, 2258, 2442, 2536
BmsI GCATC 7 cut(s) 382, 490, 634, 994, 1315, 1415, 2004
BpmI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1692
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 2 cut(s) 212, 2005
BsaAI YACGTR 3 cut(s) 1162, 1442, 2092
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 3 cut(s) 129, 1343, 2401
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
Bse1I ACTGG 4 cut(s) 733, 1211, 1502, 1788
Bse3DI GCAATG 1 cut(s) 1223
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 5 cut(s) 170, 1345, 2258, 2442, 2536
BseDI CCNNGG 3 cut(s) 129, 1343, 2401
BseGI GGATG 5 cut(s) 139, 499, 929, 1452, 2144
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
BseMI GCAATG 1 cut(s) 1223
BseMII CTCAG 4 cut(s) 249, 374, 1706, 2064
BseNI ACTGG 4 cut(s) 733, 1211, 1502, 1788
BseXI GCAGC 4 cut(s) 109, 277, 503, 2286
BsgI GTGCAG 2 cut(s) 510, 2072
Bsh1236I CGCG 1 cut(s) 2403
BsiHKAI GWGCWC 1 cut(s) 1020
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 2 cut(s) 761, 1584
BslI CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
BsmAI GTCTC 3 cut(s) 796, 1112, 1473
BsmFI GGGAC 2 cut(s) 761, 1584
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 1020, 1344, 1385
Bsp1407I TGTACA 1 cut(s) 518
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 2 cut(s) 2401, 2403
BspCNI CTCAG 4 cut(s) 250, 373, 1705, 2065
BspFNI CGCG 1 cut(s) 2403
BspLI GGNNCC 3 cut(s) 1384, 1615, 2439
BspMAI CTGCAG 2 cut(s) 295, 2412
BspPI GGATC 4 cut(s) 68, 95, 901, 2077
BspT104I TTCGAA 1 cut(s) 697
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 1 cut(s) 1223
BsrGI TGTACA 1 cut(s) 518
BsrI ACTGG 4 cut(s) 733, 1211, 1502, 1788
BssECI CCNNGG 3 cut(s) 129, 1343, 2401
BssSI CACGAG 1 cut(s) 1013
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 1013
Bst2UI CCWGG 5 cut(s) 170, 1345, 2258, 2442, 2536
Bst4CI ACNGT 4 cut(s) 775, 1707, 1792, 2431
Bst6I CTCTTC 1 cut(s) 333
BstAFI CTTAAG 1 cut(s) 1472
BstAUI TGTACA 1 cut(s) 518
BstBAI YACGTR 3 cut(s) 1162, 1442, 2092
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 4 cut(s) 1009, 1040, 1810, 1902
BstDEI CTNAG 8 cut(s) 258, 360, 485, 1692, 1998, 2032, 2073, 2339
BstDSI CCRYGG 2 cut(s) 129, 2401
BstENI CCTNNNNNAGG 3 cut(s) 674, 1623, 2208
BstF5I GGATG 5 cut(s) 139, 499, 929, 1452, 2144
BstFNI CGCG 1 cut(s) 2403
BstMAI GTCTC 3 cut(s) 796, 1112, 1473
BstMWI GCNNNNNNNGC 7 cut(s) 174, 287, 478, 1270, 1688, 1697, 1897
BstNI CCWGG 5 cut(s) 170, 1345, 2258, 2442, 2536
BstNSI RCATGY 2 cut(s) 1024, 1336
BstSCI CCNGG 5 cut(s) 168, 1343, 2256, 2440, 2534
BstSFI CTRYAG 3 cut(s) 291, 1928, 2408
BstUI CGCG 1 cut(s) 2403
BstV1I GCAGC 4 cut(s) 109, 277, 503, 2286
BstX2I RGATCY 3 cut(s) 893, 1990, 2538
BstXI CCANNNNNNTGG 1 cut(s) 1610
BstYI RGATCY 3 cut(s) 893, 1990, 2538
BsuI GTATCC 3 cut(s) 526, 562, 2408
BtgI CCRYGG 2 cut(s) 129, 2401
BtsCI GGATG 5 cut(s) 139, 499, 929, 1452, 2144
BtsIMutI CAGTG 3 cut(s) 145, 255, 2436
Cac8I GCNNGC 4 cut(s) 1009, 1040, 1810, 1902
Cfr13I GGNCC 6 cut(s) 175, 848, 1165, 1933, 2025, 2438
Cfr42I CCGCGG 1 cut(s) 2404
DdeI CTNAG 8 cut(s) 258, 360, 485, 1692, 1998, 2032, 2073, 2339
DraI TTTAAA 2 cut(s) 982, 1816
EaeI YGGCCR 2 cut(s) 877, 1943
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 669, 2214
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco47I GGWCC 3 cut(s) 1165, 1933, 2438
Eco57I CTGAAG 5 cut(s) 458, 1222, 1641, 2088, 2279
EcoNI CCTNNNNNAGG 3 cut(s) 674, 1623, 2208
EcoO109I RGGNCCY 2 cut(s) 175, 2025
EcoRI GAATTC 3 cut(s) 1389, 2233, 2516
EcoRII CCWGG 5 cut(s) 168, 1343, 2256, 2440, 2534
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 2 cut(s) 761, 1584
FauNDI CATATG 1 cut(s) 198
FbaI TGATCA 1 cut(s) 1842
Fnu4HI GCNGC 4 cut(s) 123, 291, 492, 2300
FokI GGATG 5 cut(s) 146, 486, 936, 1439, 2131
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 4 cut(s) 123, 291, 492, 2300
FspBI CTAG 3 cut(s) 213, 1961, 2542
GluI GCNGC 4 cut(s) 123, 291, 492, 2300
GsuI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
HincII GTYRAC 1 cut(s) 2532
HindII GTYRAC 1 cut(s) 2532
HindIII AAGCTT 4 cut(s) 1271, 1810, 2174, 2492
HphI GGTGA 8 cut(s) 743, 824, 959, 991, 1334, 1512, 1756, 2536
Hpy166II GTNNAC 2 cut(s) 2248, 2532
Hpy188I TCNGA 8 cut(s) 427, 435, 451, 477, 748, 770, 955, 1292
Hpy8I GTNNAC 2 cut(s) 2248, 2532
HpyCH4III ACNGT 4 cut(s) 775, 1707, 1792, 2431
HpyCH4IV ACGT 5 cut(s) 1161, 1441, 1578, 2091, 2480
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 287, 478, 1270, 1688, 1697, 1897
HpyF3I CTNAG 8 cut(s) 258, 360, 485, 1692, 1998, 2032, 2073, 2339
HpySE526I ACGT 5 cut(s) 1161, 1441, 1578, 2091, 2480
Ksp22I TGATCA 1 cut(s) 1842
KspI CCGCGG 1 cut(s) 2404
LmnI GCTCC 2 cut(s) 1388, 1619
Lsp1109I GCAGC 4 cut(s) 109, 277, 503, 2286
LweI GCATC 7 cut(s) 382, 490, 634, 994, 1315, 1415, 2004
MaeI CTAG 3 cut(s) 213, 1961, 2542
MaeII ACGT 5 cut(s) 1161, 1441, 1578, 2091, 2480
MaeIII GTNAC 7 cut(s) 239, 1062, 1518, 1744, 1855, 2039, 2365
MfeI CAATTG 2 cut(s) 526, 1594
MflI RGATCY 3 cut(s) 893, 1990, 2538
MhlI GDGCHC 3 cut(s) 1020, 1344, 1385
MlsI TGGCCA 1 cut(s) 1945
MluNI TGGCCA 1 cut(s) 1945
MlyI GAGTC 5 cut(s) 1313, 1638, 1741, 2084, 2175
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 1 cut(s) 1945
Mph1103I ATGCAT 1 cut(s) 627
MroXI GAANNNNTTC 2 cut(s) 87, 1968
MscI TGGCCA 1 cut(s) 1945
MslI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2268
Msp20I TGGCCA 1 cut(s) 1945
MspA1I CMGCKG 4 cut(s) 290, 389, 2299, 2403
MspCI CTTAAG 1 cut(s) 1472
MspR9I CCNGG 5 cut(s) 170, 1345, 2258, 2442, 2536
MunI CAATTG 2 cut(s) 526, 1594
MvaI CCWGG 5 cut(s) 170, 1345, 2258, 2442, 2536
MvnI CGCG 1 cut(s) 2403
MwoI GCNNNNNNNGC 7 cut(s) 174, 287, 478, 1270, 1688, 1697, 1897
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 198
NlaIV GGNNCC 3 cut(s) 1384, 1615, 2439
NmuCI GTSAC 3 cut(s) 1518, 1744, 1855
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 2 cut(s) 1024, 1336
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 2 cut(s) 669, 2214
PciI ACATGT 1 cut(s) 1332
PdmI GAANNNNTTC 2 cut(s) 87, 1968
PfeI GAWTC 6 cut(s) 422, 496, 760, 950, 956, 1293
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2440
PkrI GCNGC 4 cut(s) 124, 292, 493, 2301
PleI GAGTC 5 cut(s) 1312, 1637, 1741, 2083, 2174
PpsI GAGTC 5 cut(s) 1312, 1637, 1741, 2083, 2174
Ppu21I YACGTR 3 cut(s) 1162, 1442, 2092
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 3 cut(s) 321, 1155, 1922
PsiI TTATAA 1 cut(s) 1761
Psp1406I AACGTT 1 cut(s) 2480
Psp6I CCWGG 5 cut(s) 168, 1343, 2256, 2440, 2534
PspGI CCWGG 5 cut(s) 168, 1343, 2256, 2440, 2534
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 3 cut(s) 1384, 1615, 2439
PspPI GGNCC 6 cut(s) 175, 848, 1165, 1933, 2025, 2438
PstI CTGCAG 2 cut(s) 295, 2412
PsuI RGATCY 3 cut(s) 893, 1990, 2538
PvuII CAGCTG 3 cut(s) 290, 389, 2299
RseI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2268
SacII CCGCGG 1 cut(s) 2404
SatI GCNGC 4 cut(s) 123, 291, 492, 2300
Sau96I GGNCC 6 cut(s) 175, 848, 1165, 1933, 2025, 2438
ScaI AGTACT 1 cut(s) 2065
SchI GAGTC 5 cut(s) 1313, 1638, 1741, 2084, 2175
ScrFI CCNGG 5 cut(s) 170, 1345, 2258, 2442, 2536
SduI GDGCHC 3 cut(s) 1020, 1344, 1385
SfaNI GCATC 7 cut(s) 382, 490, 634, 994, 1315, 1415, 2004
SfcI CTRYAG 3 cut(s) 291, 1928, 2408
SfiI GGCCNNNNNGGCC 1 cut(s) 174
Sfr303I CCGCGG 1 cut(s) 2404
SfuI TTCGAA 1 cut(s) 697
SgrBI CCGCGG 1 cut(s) 2404
SinI GGWCC 3 cut(s) 1165, 1933, 2438
SmiMI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2268
SmlI CTYRAG 3 cut(s) 191, 1472, 2020
SmoI CTYRAG 3 cut(s) 191, 1472, 2020
SseBI AGGCCT 2 cut(s) 669, 2214
SsiI CCGC 2 cut(s) 2401, 2403
SspI AATATT 1 cut(s) 1642
SspMI CTAG 3 cut(s) 213, 1961, 2542
StuI AGGCCT 2 cut(s) 669, 2214
StyD4I CCNGG 5 cut(s) 168, 1343, 2256, 2440, 2534
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 4 cut(s) 775, 1707, 1792, 2431
TaiI ACGT 5 cut(s) 1164, 1444, 1581, 2094, 2483
TaqI TCGA 4 cut(s) 547, 697, 1338, 2266
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 6 cut(s) 51, 249, 518, 1707, 1908, 2063
TfiI GAWTC 6 cut(s) 422, 496, 760, 950, 956, 1293
TscAI CASTG 3 cut(s) 145, 262, 2436
TseFI GTSAC 3 cut(s) 1518, 1744, 1855
TseI GCWGC 4 cut(s) 122, 290, 491, 2299
Tsp45I GTSAC 3 cut(s) 1518, 1744, 1855
TspDTI ATGAA 9 cut(s) 479, 594, 612, 897, 1118, 1164, 1365, 1441, 2252
TspGWI ACGGA 3 cut(s) 53, 654, 1475
TspRI CASTG 3 cut(s) 145, 262, 2436
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1165, 1933, 2438
VspI ATTAAT 3 cut(s) 321, 1155, 1922
XagI CCTNNNNNAGG 3 cut(s) 674, 1623, 2208
XceI RCATGY 2 cut(s) 1024, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1730
XmnI GAANNNNTTC 2 cut(s) 87, 1968
XspI CTAG 3 cut(s) 213, 1961, 2542
ZrmI AGTACT 1 cut(s) 2065
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.