Rw3G022020

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001584
Physical Location & Seq
Forward (+)
26812727 .. 26825814
13088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022020.1

Sequence Viewer

Length: 2898 bp
ATGGCTGTGGGGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTTCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATAGTTACTCCAAGTACATCACTGAGCATGGAGGAAGAACAAATGCTTTCACAGCTGCAGAACACACCAACTTTTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCTGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTGAGTGCAGCGAATCATCCTTACCATAAATTTTGTACAGGCAATTGGGATACTTTGGAGGTTCGACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGAGCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGCGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATGCAAACATGTTCGAGCCCTGGTATGAAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGACAGCATCTTCCCCAAATAAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCAACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATACAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCCAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGGAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTCAGGTTGCTGAACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTCTTGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGGAGTACCAAAATTACAAATTTCAACAGCCTTATGAGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCAATGATGCTCTCAAGGGCCTTCTTAGAGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATATTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGACAGGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATGAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCGGCTGTTTGTTCTTATTGCAAAGCAACCAGCCTTTCACCAACTTAGATCAGTCGAGCAACTTGGTTACATAACTCGTCTTTCACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGCTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

965

Amino Acids

111.53

Weight (kDa)

6.09

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 1.2e-36 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 1.2e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 655 8.2e-103 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 660 - 846 3.6e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 771 - 866 6.8e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1761, 2694
AccB1I GGYRCC 1 cut(s) 2716
AccII CGCG 1 cut(s) 2403
AciI CCGC 2 cut(s) 2401, 2403
AclI AACGTT 1 cut(s) 2480
AclWI GGATC 4 cut(s) 68, 95, 901, 2077
AcoI YGGCCR 2 cut(s) 877, 1943
AcuI CTGAAG 4 cut(s) 458, 1222, 1641, 2088
AfiI CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AjnI CCWGG 4 cut(s) 168, 1343, 2256, 2440
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 3 cut(s) 796, 1112, 1473
AlwI GGATC 4 cut(s) 68, 95, 901, 2077
ApeKI GCWGC 5 cut(s) 122, 290, 491, 2651, 2662
ArsI GACNNNNNNTTYG 2 cut(s) 1563, 1595
AseI ATTAAT 3 cut(s) 321, 1155, 1922
Asp700I GAANNNNTTC 2 cut(s) 87, 1968
AspS9I GGNCC 6 cut(s) 175, 848, 1933, 2025, 2438, 2616
AsuHPI GGTGA 8 cut(s) 743, 824, 959, 991, 1334, 1512, 1756, 2324
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 3 cut(s) 1933, 2438, 2616
BaeGI GKGCMC 1 cut(s) 2719
BalI TGGCCA 1 cut(s) 1945
BanI GGYRCC 1 cut(s) 2716
BanII GRGCYC 2 cut(s) 1344, 1385
BauI CACGAG 1 cut(s) 1013
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 5 cut(s) 109, 277, 503, 2663, 2674
BccI CCATC 7 cut(s) 158, 323, 446, 918, 1945, 2152, 2606
BciT130I CCWGG 4 cut(s) 170, 1345, 2258, 2442
BciVI GTATCC 3 cut(s) 526, 562, 2408
BclI TGATCA 1 cut(s) 1842
BcoDI GTCTC 3 cut(s) 796, 1112, 1473
BfaI CTAG 2 cut(s) 213, 1961
BfmI CTRYAG 3 cut(s) 291, 1928, 2408
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 3 cut(s) 526, 562, 2408
BglI GCCNNNNNGGC 1 cut(s) 174
BglII AGATCT 1 cut(s) 1990
BisI GCNGC 5 cut(s) 123, 291, 492, 2652, 2663
BlsI GCNGC 5 cut(s) 124, 292, 493, 2653, 2664
BmcAI AGTACT 1 cut(s) 2065
Bme1390I CCNGG 4 cut(s) 170, 1345, 2258, 2442
Bme18I GGWCC 3 cut(s) 1933, 2438, 2616
BmgT120I GGNCC 6 cut(s) 175, 848, 1933, 2025, 2438, 2616
BmiI GGNNCC 6 cut(s) 1384, 1615, 2439, 2617, 2618, 2718
BmrFI CCNGG 4 cut(s) 170, 1345, 2258, 2442
BmrI ACTGGG 1 cut(s) 2789
BmsI GCATC 8 cut(s) 382, 490, 634, 994, 1315, 1415, 2004, 2786
BmuI ACTGGG 1 cut(s) 2789
BpmI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1692
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 5 cut(s) 212, 2005, 2576, 2593, 2606
Bsa29I ATCGAT 2 cut(s) 2544, 2820
BsaAI YACGTR 3 cut(s) 1162, 1442, 2092
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 3 cut(s) 129, 1343, 2401
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
Bse1I ACTGG 5 cut(s) 733, 1211, 1502, 1788, 2795
Bse3DI GCAATG 2 cut(s) 1223, 2533
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 4 cut(s) 170, 1345, 2258, 2442
BseCI ATCGAT 2 cut(s) 2544, 2820
BseDI CCNNGG 3 cut(s) 129, 1343, 2401
BseGI GGATG 5 cut(s) 139, 499, 929, 1452, 2144
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
BseMI GCAATG 2 cut(s) 1223, 2533
BseMII CTCAG 7 cut(s) 249, 374, 476, 1706, 2064, 2648, 2760
BseNI ACTGG 5 cut(s) 733, 1211, 1502, 1788, 2795
BseSI GKGCMC 1 cut(s) 2719
BseXI GCAGC 5 cut(s) 109, 277, 503, 2663, 2674
BsgI GTGCAG 2 cut(s) 510, 2072
Bsh1236I CGCG 1 cut(s) 2403
BshNI GGYRCC 1 cut(s) 2716
BshVI ATCGAT 2 cut(s) 2544, 2820
BsiHKAI GWGCWC 1 cut(s) 1020
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1584, 2629
BslI CCNNNNNNNGG 5 cut(s) 232, 676, 841, 1625, 2210
BsmAI GTCTC 3 cut(s) 796, 1112, 1473
BsmFI GGGAC 3 cut(s) 761, 1584, 2629
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 4 cut(s) 1020, 1344, 1385, 2719
Bsp1407I TGTACA 1 cut(s) 518
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 2 cut(s) 2401, 2403
BspCNI CTCAG 7 cut(s) 250, 373, 477, 1705, 2065, 2649, 2761
BspDI ATCGAT 2 cut(s) 2544, 2820
BspFNI CGCG 1 cut(s) 2403
BspLI GGNNCC 6 cut(s) 1384, 1615, 2439, 2617, 2618, 2718
BspMAI CTGCAG 2 cut(s) 295, 2412
BspPI GGATC 4 cut(s) 68, 95, 901, 2077
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2716
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 2 cut(s) 1223, 2533
BsrGI TGTACA 1 cut(s) 518
BsrI ACTGG 5 cut(s) 733, 1211, 1502, 1788, 2795
BssECI CCNNGG 3 cut(s) 129, 1343, 2401
BssSI CACGAG 1 cut(s) 1013
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 1013
Bst2UI CCWGG 4 cut(s) 170, 1345, 2258, 2442
Bst4CI ACNGT 5 cut(s) 775, 1707, 1792, 2431, 2812
Bst6I CTCTTC 2 cut(s) 12, 333
BstAFI CTTAAG 1 cut(s) 1472
BstAPI GCANNNNNTGC 1 cut(s) 2648
BstAUI TGTACA 1 cut(s) 518
BstBAI YACGTR 3 cut(s) 1162, 1442, 2092
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 4 cut(s) 1009, 1040, 1810, 1902
BstDSI CCRYGG 2 cut(s) 129, 2401
BstENI CCTNNNNNAGG 3 cut(s) 674, 1623, 2208
BstF5I GGATG 5 cut(s) 139, 499, 929, 1452, 2144
BstFNI CGCG 1 cut(s) 2403
BstMAI GTCTC 3 cut(s) 796, 1112, 1473
BstMWI GCNNNNNNNGC 9 cut(s) 174, 287, 478, 1270, 1688, 1697, 1897, 2560, 2648
BstNI CCWGG 4 cut(s) 170, 1345, 2258, 2442
BstNSI RCATGY 2 cut(s) 1024, 1336
BstSCI CCNGG 4 cut(s) 168, 1343, 2256, 2440
BstSFI CTRYAG 3 cut(s) 291, 1928, 2408
BstSLI GKGCMC 1 cut(s) 2719
BstUI CGCG 1 cut(s) 2403
BstV1I GCAGC 5 cut(s) 109, 277, 503, 2663, 2674
BstX2I RGATCY 2 cut(s) 893, 1990
BstXI CCANNNNNNTGG 1 cut(s) 1610
BstYI RGATCY 2 cut(s) 893, 1990
Bsu15I ATCGAT 2 cut(s) 2544, 2820
BsuI GTATCC 3 cut(s) 526, 562, 2408
BsuTUI ATCGAT 2 cut(s) 2544, 2820
BtgI CCRYGG 2 cut(s) 129, 2401
BtsCI GGATG 5 cut(s) 139, 499, 929, 1452, 2144
BtsIMutI CAGTG 6 cut(s) 145, 255, 2436, 2654, 2802, 2808
Cac8I GCNNGC 4 cut(s) 1009, 1040, 1810, 1902
Cfr13I GGNCC 6 cut(s) 175, 848, 1933, 2025, 2438, 2616
Cfr42I CCGCGG 1 cut(s) 2404
ClaI ATCGAT 2 cut(s) 2544, 2820
DraI TTTAAA 2 cut(s) 982, 1816
EaeI YGGCCR 2 cut(s) 877, 1943
Eam1104I CTCTTC 2 cut(s) 12, 333
EarI CTCTTC 2 cut(s) 12, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 669, 2214
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco47I GGWCC 3 cut(s) 1933, 2438, 2616
Eco57I CTGAAG 4 cut(s) 458, 1222, 1641, 2088
EcoNI CCTNNNNNAGG 3 cut(s) 674, 1623, 2208
EcoO109I RGGNCCY 3 cut(s) 175, 2025, 2616
EcoRI GAATTC 3 cut(s) 1389, 2233, 2516
EcoRII CCWGG 4 cut(s) 168, 1343, 2256, 2440
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 627, 2779
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 4 cut(s) 636, 668, 2861, 2893
FaqI GGGAC 3 cut(s) 761, 1584, 2629
FauNDI CATATG 1 cut(s) 198
FbaI TGATCA 1 cut(s) 1842
Fnu4HI GCNGC 5 cut(s) 123, 291, 492, 2652, 2663
FokI GGATG 5 cut(s) 146, 486, 936, 1439, 2131
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 5 cut(s) 123, 291, 492, 2652, 2663
FspBI CTAG 2 cut(s) 213, 1961
GluI GCNGC 5 cut(s) 123, 291, 492, 2652, 2663
GsuI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
HindIII AAGCTT 5 cut(s) 1271, 1810, 2174, 2492, 2552
HphI GGTGA 8 cut(s) 743, 824, 959, 991, 1334, 1512, 1756, 2324
Hpy166II GTNNAC 2 cut(s) 2248, 2741
Hpy8I GTNNAC 2 cut(s) 2248, 2741
HpyCH4III ACNGT 5 cut(s) 775, 1707, 1792, 2431, 2812
HpyCH4IV ACGT 5 cut(s) 1161, 1441, 1578, 2091, 2480
HpyF10VI GCNNNNNNNGC 9 cut(s) 174, 287, 478, 1270, 1688, 1697, 1897, 2560, 2648
HpySE526I ACGT 5 cut(s) 1161, 1441, 1578, 2091, 2480
KflI GGGWCCC 1 cut(s) 2616
Ksp22I TGATCA 1 cut(s) 1842
KspI CCGCGG 1 cut(s) 2404
LmnI GCTCC 3 cut(s) 1388, 1619, 2795
Lsp1109I GCAGC 5 cut(s) 109, 277, 503, 2663, 2674
LweI GCATC 8 cut(s) 382, 490, 634, 994, 1315, 1415, 2004, 2786
MaeI CTAG 2 cut(s) 213, 1961
MaeII ACGT 5 cut(s) 1161, 1441, 1578, 2091, 2480
MaeIII GTNAC 8 cut(s) 239, 1062, 1518, 1744, 1855, 2039, 2360, 2844
MfeI CAATTG 2 cut(s) 526, 1594
MflI RGATCY 2 cut(s) 893, 1990
MhlI GDGCHC 4 cut(s) 1020, 1344, 1385, 2719
MlsI TGGCCA 1 cut(s) 1945
MluNI TGGCCA 1 cut(s) 1945
MlyI GAGTC 5 cut(s) 1313, 1638, 1741, 2084, 2175
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 1 cut(s) 1945
Mph1103I ATGCAT 2 cut(s) 627, 2779
MroXI GAANNNNTTC 2 cut(s) 87, 1968
MscI TGGCCA 1 cut(s) 1945
MslI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2268
Msp20I TGGCCA 1 cut(s) 1945
MspA1I CMGCKG 3 cut(s) 290, 389, 2403
MspCI CTTAAG 1 cut(s) 1472
MspR9I CCNGG 4 cut(s) 170, 1345, 2258, 2442
MunI CAATTG 2 cut(s) 526, 1594
MvaI CCWGG 4 cut(s) 170, 1345, 2258, 2442
MvnI CGCG 1 cut(s) 2403
MwoI GCNNNNNNNGC 9 cut(s) 174, 287, 478, 1270, 1688, 1697, 1897, 2560, 2648
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 198
NlaIV GGNNCC 6 cut(s) 1384, 1615, 2439, 2617, 2618, 2718
NmuCI GTSAC 4 cut(s) 1518, 1744, 1855, 2844
NsiI ATGCAT 2 cut(s) 627, 2779
NspI RCATGY 2 cut(s) 1024, 1336
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 2 cut(s) 669, 2214
PciI ACATGT 1 cut(s) 1332
PdmI GAANNNNTTC 2 cut(s) 87, 1968
PfeI GAWTC 7 cut(s) 422, 496, 760, 950, 956, 1293, 2755
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2440
PkrI GCNGC 5 cut(s) 124, 292, 493, 2653, 2664
PleI GAGTC 5 cut(s) 1312, 1637, 1741, 2083, 2174
PpsI GAGTC 5 cut(s) 1312, 1637, 1741, 2083, 2174
Ppu21I YACGTR 3 cut(s) 1162, 1442, 2092
PpuMI RGGWCCY 1 cut(s) 2616
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 3 cut(s) 321, 1155, 1922
PsiI TTATAA 2 cut(s) 1761, 2694
Psp1406I AACGTT 1 cut(s) 2480
Psp5II RGGWCCY 1 cut(s) 2616
Psp6I CCWGG 4 cut(s) 168, 1343, 2256, 2440
PspGI CCWGG 4 cut(s) 168, 1343, 2256, 2440
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1384, 1615, 2439, 2617, 2618, 2718
PspPI GGNCC 6 cut(s) 175, 848, 1933, 2025, 2438, 2616
PspPPI RGGWCCY 1 cut(s) 2616
PstI CTGCAG 2 cut(s) 295, 2412
PsuI RGATCY 2 cut(s) 893, 1990
PvuII CAGCTG 2 cut(s) 290, 389
RseI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2268
SacII CCGCGG 1 cut(s) 2404
SatI GCNGC 5 cut(s) 123, 291, 492, 2652, 2663
Sau96I GGNCC 6 cut(s) 175, 848, 1933, 2025, 2438, 2616
ScaI AGTACT 1 cut(s) 2065
SchI GAGTC 5 cut(s) 1313, 1638, 1741, 2084, 2175
ScrFI CCNGG 4 cut(s) 170, 1345, 2258, 2442
SduI GDGCHC 4 cut(s) 1020, 1344, 1385, 2719
SfaNI GCATC 8 cut(s) 382, 490, 634, 994, 1315, 1415, 2004, 2786
SfcI CTRYAG 3 cut(s) 291, 1928, 2408
SfiI GGCCNNNNNGGCC 1 cut(s) 174
Sfr303I CCGCGG 1 cut(s) 2404
SfuI TTCGAA 1 cut(s) 697
SgrBI CCGCGG 1 cut(s) 2404
SinI GGWCC 3 cut(s) 1933, 2438, 2616
SmiMI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2268
SmlI CTYRAG 6 cut(s) 191, 1472, 2020, 2555, 2572, 2621
SmoI CTYRAG 6 cut(s) 191, 1472, 2020, 2555, 2572, 2621
SseBI AGGCCT 2 cut(s) 669, 2214
SsiI CCGC 2 cut(s) 2401, 2403
SspI AATATT 2 cut(s) 1171, 1642
SspMI CTAG 2 cut(s) 213, 1961
StuI AGGCCT 2 cut(s) 669, 2214
StyD4I CCNGG 4 cut(s) 168, 1343, 2256, 2440
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 5 cut(s) 775, 1707, 1792, 2431, 2812
TaiI ACGT 5 cut(s) 1164, 1444, 1581, 2094, 2483
TaqI TCGA 7 cut(s) 547, 697, 1338, 2266, 2349, 2544, 2820
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 6 cut(s) 51, 249, 518, 1707, 1908, 2063
TfiI GAWTC 7 cut(s) 422, 496, 760, 950, 956, 1293, 2755
TscAI CASTG 6 cut(s) 145, 262, 2436, 2661, 2802, 2815
TseFI GTSAC 4 cut(s) 1518, 1744, 1855, 2844
TseI GCWGC 5 cut(s) 122, 290, 491, 2651, 2662
Tsp45I GTSAC 4 cut(s) 1518, 1744, 1855, 2844
TspGWI ACGGA 3 cut(s) 53, 654, 1475
TspRI CASTG 6 cut(s) 145, 262, 2436, 2661, 2802, 2815
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1933, 2438, 2616
VspI ATTAAT 3 cut(s) 321, 1155, 1922
XagI CCTNNNNNAGG 3 cut(s) 674, 1623, 2208
XceI RCATGY 2 cut(s) 1024, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1730
XmnI GAANNNNTTC 2 cut(s) 87, 1968
XspI CTAG 2 cut(s) 213, 1961
ZrmI AGTACT 1 cut(s) 2065
Zsp2I ATGCAT 2 cut(s) 627, 2779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.