RchiOBHm_Chr3g0482171

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001584
Physical Location & Seq
Forward (+)
28313886 .. 28327636
13751 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44707

Sequence Viewer

Length: 2958 bp
ATGGCTGGGGCGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTTCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGGCAACTGCTCTTTCATCTCAGACTCGTTTCTCTGTTTCAGTGGATACGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGGTAGCTACTTGAAGTACATCAGCGAGCATGGAGGAACAGGAAAAGCTTTCACTGCTGCAGAACACACCAGCTATCGTTTCGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAGCGAGGAAAATTAAAGCTGTTAATTCAGCAAATCTGAAAAATCTGTTGTCCGATGACTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAGTTGGCATACTTTGGCGGTTCGACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTAAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCGTGTAGGTATCTTGGTCACCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGCTGGCTGGTGAATCAGATTCTACTTTGGACTTTTCTTTCTTTGAAGTATATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACGTTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTATTTGTGAGACAAAGTTTCATTATCAGGACAAAATTGAGCCTATTAATTACGTGGTCAGTATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATTGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAAAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGTGAATTTACACCTACCAGCACGTAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTCATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATATACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAACGGTCACAAAGGAGTACCAAAATTACAAATTTGAACAGTCCTATGAGCAGGCTATTTACTATTGCTCATTAGTTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTACTCATCTTGAAGTTGAAGATCTTGCTAATTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCAAGTAGAATATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCGAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTATTTGTTCTTATTGCAAAGGAACCAGCCTTTCACCAACTTAGAATAGTTGAGCAACTTGGTTACATAACTCGTCTTTTACAGAGGAATGATTGTGGTATCCGTGGACTGCAGTTCATTATACAATCCACAGTGAAGGGTCCAGGACGTATTGATTTGAGAGTGGAGGAATTTCTCAAGACGTTTGAGAGTAAGCTTTATGAGATGACAAATAACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACCAGAACTTGAGGGAAGAAGCTGAATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGCTCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTTCAATGAGCATATAAAAGTTGGGGCACCTCATAAGAGGACATTGAGTGTACGAGTATATGGGAATCGTCATTCATTTGAGTATGCATCAGATAAAAGATCCCTAGTGCAACCTTGCACCGTCAAAATCGATGATATTTTCGCTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAGCTCCGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

985

Amino Acids

113.44

Weight (kDa)

6.31

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 53 - 179 9.6e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 207 - 384 3.1e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 392 - 675 6.8e-103 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 680 - 866 1.2e-09 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 791 - 886 5.1e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1821
AccB1I GGYRCC 1 cut(s) 2776
AciI CCGC 1 cut(s) 590
AclWI GGATC 5 cut(s) 68, 95, 949, 2137, 2844
AcoI YGGCCR 2 cut(s) 925, 2003
AcuI CTGAAG 4 cut(s) 506, 1270, 2148, 2339
AfiI CCNNNNNNNGG 8 cut(s) 280, 281, 504, 724, 889, 1397, 2270, 2918
AflII CTTAAG 1 cut(s) 1520
AflIII ACRYGT 2 cut(s) 164, 1380
AhlI ACTAGT 1 cut(s) 1697
AjnI CCWGG 4 cut(s) 216, 1391, 2316, 2500
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
Alw26I GTCTC 2 cut(s) 1160, 1521
AlwI GGATC 5 cut(s) 68, 95, 949, 2137, 2844
ApeKI GCWGC 5 cut(s) 170, 338, 539, 2711, 2722
AseI ATTAAT 2 cut(s) 369, 1203
AspS9I GGNCC 6 cut(s) 223, 896, 1993, 2085, 2498, 2676
AsuC2I CCSGG 1 cut(s) 1679
AsuHPI GGTGA 9 cut(s) 791, 872, 908, 1007, 1039, 1382, 1572, 1816, 2384
AsuII TTCGAA 1 cut(s) 745
AvaII GGWCC 3 cut(s) 1993, 2498, 2676
BaeGI GKGCMC 1 cut(s) 2779
BalI TGGCCA 1 cut(s) 2005
BanI GGYRCC 1 cut(s) 2776
BanII GRGCYC 2 cut(s) 1392, 1433
BbvCI CCTCAGC 1 cut(s) 1752
BbvI GCAGC 5 cut(s) 157, 325, 551, 2723, 2734
BccI CCATC 6 cut(s) 206, 371, 966, 2005, 2212, 2666
BceAI ACGGC 1 cut(s) 883
BciT130I CCWGG 4 cut(s) 218, 1393, 2318, 2502
BciVI GTATCC 3 cut(s) 154, 610, 2468
BclI TGATCA 1 cut(s) 1902
BcnI CCSGG 1 cut(s) 1679
BcoDI GTCTC 2 cut(s) 1160, 1521
BcuI ACTAGT 1 cut(s) 1697
BfaI CTAG 4 cut(s) 261, 1698, 2021, 2855
BfmI CTRYAG 4 cut(s) 339, 444, 1988, 2468
BfrI CTTAAG 1 cut(s) 1520
BfuI GTATCC 3 cut(s) 154, 610, 2468
BglI GCCNNNNNGGC 1 cut(s) 222
BglII AGATCT 1 cut(s) 2050
BisI GCNGC 5 cut(s) 171, 339, 540, 2712, 2723
BlsI GCNGC 5 cut(s) 172, 340, 541, 2713, 2724
BmcAI AGTACT 1 cut(s) 2125
Bme1390I CCNGG 5 cut(s) 218, 1393, 1679, 2318, 2502
Bme18I GGWCC 3 cut(s) 1993, 2498, 2676
BmgT120I GGNCC 6 cut(s) 223, 896, 1993, 2085, 2498, 2676
BmiI GGNNCC 7 cut(s) 1432, 1675, 2382, 2499, 2677, 2678, 2778
BmrFI CCNGG 5 cut(s) 218, 1393, 1679, 2318, 2502
BmsI GCATC 7 cut(s) 430, 538, 682, 1042, 1463, 2064, 2846
BpmI CTGGAG 5 cut(s) 239, 526, 824, 855, 1417
Bpu10I CCTNAGC 1 cut(s) 1752
Bpu14I TTCGAA 1 cut(s) 745
BpuEI CTTGAG 6 cut(s) 260, 2065, 2519, 2636, 2653, 2666
BpuMI CCSGG 1 cut(s) 1679
Bsa29I ATCGAT 2 cut(s) 2604, 2880
BsaAI YACGTR 3 cut(s) 165, 1210, 1490
BsaJI CCNNGG 5 cut(s) 177, 1391, 1506, 1677, 2461
BsaWI WCCGGW 1 cut(s) 2939
Bsc4I CCNNNNNNNGG 8 cut(s) 280, 281, 504, 724, 889, 1397, 2270, 2918
Bse1I ACTGG 5 cut(s) 509, 781, 1259, 1562, 1848
Bse3DI GCAATG 2 cut(s) 1271, 2593
BseBI CCWGG 4 cut(s) 218, 1393, 2318, 2502
BseCI ATCGAT 2 cut(s) 2604, 2880
BseDI CCNNGG 5 cut(s) 177, 1391, 1506, 1677, 2461
BseGI GGATG 5 cut(s) 187, 547, 977, 1500, 2204
BseLI CCNNNNNNNGG 8 cut(s) 280, 281, 504, 724, 889, 1397, 2270, 2918
BseMI GCAATG 2 cut(s) 1271, 2593
BseMII CTCAG 5 cut(s) 149, 422, 1743, 2124, 2708
BseNI ACTGG 5 cut(s) 509, 781, 1259, 1562, 1848
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2779
BseXI GCAGC 5 cut(s) 157, 325, 551, 2723, 2734
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 558
BshNI GGYRCC 1 cut(s) 2776
BshVI ATCGAT 2 cut(s) 2604, 2880
BsiSI CCGG 2 cut(s) 1679, 2940
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 809, 1644, 2689
BslI CCNNNNNNNGG 8 cut(s) 280, 281, 504, 724, 889, 1397, 2270, 2918
BsmAI GTCTC 2 cut(s) 1160, 1521
BsmFI GGGAC 3 cut(s) 809, 1644, 2689
Bsp119I TTCGAA 1 cut(s) 745
Bsp1286I GDGCHC 3 cut(s) 1392, 1433, 2779
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 1 cut(s) 590
BspCNI CTCAG 5 cut(s) 148, 421, 1744, 2125, 2709
BspDI ATCGAT 2 cut(s) 2604, 2880
BspLI GGNNCC 7 cut(s) 1432, 1675, 2382, 2499, 2677, 2678, 2778
BspMAI CTGCAG 2 cut(s) 343, 2472
BspPI GGATC 5 cut(s) 68, 95, 949, 2137, 2844
BspT104I TTCGAA 1 cut(s) 745
BspT107I GGYRCC 1 cut(s) 2776
BspTI CTTAAG 1 cut(s) 1520
BsrDI GCAATG 2 cut(s) 1271, 2593
BsrI ACTGG 5 cut(s) 509, 781, 1259, 1562, 1848
BssECI CCNNGG 5 cut(s) 177, 1391, 1506, 1677, 2461
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 4 cut(s) 218, 1393, 2318, 2502
Bst4CI ACNGT 7 cut(s) 823, 1767, 1852, 1915, 1950, 2491, 2872
Bst6I CTCTTC 2 cut(s) 12, 381
BstAFI CTTAAG 1 cut(s) 1520
BstAPI GCANNNNNTGC 1 cut(s) 2708
BstBAI YACGTR 3 cut(s) 165, 1210, 1490
BstBI TTCGAA 1 cut(s) 745
BstC8I GCNNGC 6 cut(s) 308, 990, 1057, 1088, 1870, 1962
BstDEI CTNAG 8 cut(s) 135, 408, 533, 1752, 2092, 2133, 2399, 2717
BstDSI CCRYGG 3 cut(s) 177, 1506, 2461
BstEII GGTNACC 1 cut(s) 914
BstENI CCTNNNNNAGG 2 cut(s) 722, 2268
BstF5I GGATG 5 cut(s) 187, 547, 977, 1500, 2204
BstMAI GTCTC 2 cut(s) 1160, 1521
BstMWI GCNNNNNNNGC 8 cut(s) 222, 335, 526, 1318, 1748, 1757, 2620, 2708
BstNI CCWGG 4 cut(s) 218, 1393, 2318, 2502
BstNSI RCATGY 2 cut(s) 1072, 1384
BstPI GGTNACC 1 cut(s) 914
BstSCI CCNGG 5 cut(s) 216, 1391, 1677, 2316, 2500
BstSFI CTRYAG 4 cut(s) 339, 444, 1988, 2468
BstSLI GKGCMC 1 cut(s) 2779
BstV1I GCAGC 5 cut(s) 157, 325, 551, 2723, 2734
BstX2I RGATCY 3 cut(s) 941, 2050, 2849
BstXI CCANNNNNNTGG 1 cut(s) 1670
BstYI RGATCY 3 cut(s) 941, 2050, 2849
Bsu15I ATCGAT 2 cut(s) 2604, 2880
BsuI GTATCC 3 cut(s) 154, 610, 2468
BsuTUI ATCGAT 2 cut(s) 2604, 2880
BtgI CCRYGG 3 cut(s) 177, 1506, 2461
BtsCI GGATG 5 cut(s) 187, 547, 977, 1500, 2204
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 5 cut(s) 162, 193, 333, 2496, 2714
Cac8I GCNNGC 6 cut(s) 308, 990, 1057, 1088, 1870, 1962
Cfr13I GGNCC 6 cut(s) 223, 896, 1993, 2085, 2498, 2676
ClaI ATCGAT 2 cut(s) 2604, 2880
DdeI CTNAG 8 cut(s) 135, 408, 533, 1752, 2092, 2133, 2399, 2717
DraI TTTAAA 1 cut(s) 1876
EaeI YGGCCR 2 cut(s) 925, 2003
Eam1104I CTCTTC 2 cut(s) 12, 381
EarI CTCTTC 2 cut(s) 12, 381
Eco130I CCWWGG 1 cut(s) 177
Eco147I AGGCCT 2 cut(s) 717, 2274
Eco24I GRGCYC 2 cut(s) 1392, 1433
Eco47I GGWCC 3 cut(s) 1993, 2498, 2676
Eco57I CTGAAG 4 cut(s) 506, 1270, 2148, 2339
Eco91I GGTNACC 1 cut(s) 914
EcoNI CCTNNNNNAGG 2 cut(s) 722, 2268
EcoO109I RGGNCCY 3 cut(s) 223, 2085, 2676
EcoO65I GGTNACC 1 cut(s) 914
EcoRI GAATTC 3 cut(s) 1437, 2293, 2576
EcoRII CCWGG 4 cut(s) 216, 1391, 2316, 2500
EcoT14I CCWWGG 1 cut(s) 177
EcoT22I ATGCAT 2 cut(s) 675, 2839
EcoT38I GRGCYC 2 cut(s) 1392, 1433
ErhI CCWWGG 1 cut(s) 177
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 3 cut(s) 809, 1644, 2689
FauNDI CATATG 2 cut(s) 246, 1585
FbaI TGATCA 1 cut(s) 1902
Fnu4HI GCNGC 5 cut(s) 171, 339, 540, 2712, 2723
FokI GGATG 5 cut(s) 194, 534, 984, 1487, 2191
FriOI GRGCYC 2 cut(s) 1392, 1433
Fsp4HI GCNGC 5 cut(s) 171, 339, 540, 2712, 2723
FspBI CTAG 4 cut(s) 261, 1698, 2021, 2855
GluI GCNGC 5 cut(s) 171, 339, 540, 2712, 2723
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 5 cut(s) 239, 526, 824, 855, 1417
HapII CCGG 2 cut(s) 1679, 2940
HindIII AAGCTT 6 cut(s) 327, 1319, 1870, 2234, 2552, 2612
HpaII CCGG 2 cut(s) 1679, 2940
HphI GGTGA 9 cut(s) 791, 872, 908, 1007, 1039, 1382, 1572, 1816, 2384
Hpy166II GTNNAC 3 cut(s) 2308, 2465, 2801
Hpy8I GTNNAC 3 cut(s) 2308, 2465, 2801
HpyCH4III ACNGT 7 cut(s) 823, 1767, 1852, 1915, 1950, 2491, 2872
HpyCH4IV ACGT 7 cut(s) 164, 1101, 1209, 1489, 1638, 2506, 2540
HpyF10VI GCNNNNNNNGC 8 cut(s) 222, 335, 526, 1318, 1748, 1757, 2620, 2708
HpyF3I CTNAG 8 cut(s) 135, 408, 533, 1752, 2092, 2133, 2399, 2717
HpySE526I ACGT 7 cut(s) 164, 1101, 1209, 1489, 1638, 2506, 2540
KflI GGGWCCC 1 cut(s) 2676
Ksp22I TGATCA 1 cut(s) 1902
LmnI GCTCC 3 cut(s) 1436, 1679, 2942
Lsp1109I GCAGC 5 cut(s) 157, 325, 551, 2723, 2734
LweI GCATC 7 cut(s) 430, 538, 682, 1042, 1463, 2064, 2846
MaeI CTAG 4 cut(s) 261, 1698, 2021, 2855
MaeII ACGT 7 cut(s) 164, 1101, 1209, 1489, 1638, 2506, 2540
MaeIII GTNAC 8 cut(s) 914, 1110, 1578, 1804, 1915, 2099, 2420, 2904
MboII GAAGA 9 cut(s) 29, 398, 659, 1336, 1449, 1873, 2060, 2289, 2651
MfeI CAATTG 1 cut(s) 1654
MflI RGATCY 3 cut(s) 941, 2050, 2849
MhlI GDGCHC 3 cut(s) 1392, 1433, 2779
MlsI TGGCCA 1 cut(s) 2005
MluNI TGGCCA 1 cut(s) 2005
MlyI GAGTC 4 cut(s) 133, 1361, 2144, 2235
Mox20I TGGCCA 1 cut(s) 2005
Mph1103I ATGCAT 2 cut(s) 675, 2839
MscI TGGCCA 1 cut(s) 2005
MslI CAYNNNNRTG 4 cut(s) 534, 970, 1668, 2328
Msp20I TGGCCA 1 cut(s) 2005
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 1520
MspI CCGG 2 cut(s) 1679, 2940
MspR9I CCNGG 5 cut(s) 218, 1393, 1679, 2318, 2502
MunI CAATTG 1 cut(s) 1654
MvaI CCWGG 4 cut(s) 218, 1393, 2318, 2502
MwoI GCNNNNNNNGC 8 cut(s) 222, 335, 526, 1318, 1748, 1757, 2620, 2708
NciI CCSGG 1 cut(s) 1679
NcoI CCATGG 1 cut(s) 177
NdeI CATATG 2 cut(s) 246, 1585
NlaIV GGNNCC 7 cut(s) 1432, 1675, 2382, 2499, 2677, 2678, 2778
NmuCI GTSAC 5 cut(s) 914, 1578, 1804, 1915, 2904
NsiI ATGCAT 2 cut(s) 675, 2839
NspI RCATGY 2 cut(s) 1072, 1384
NspV TTCGAA 1 cut(s) 745
PceI AGGCCT 2 cut(s) 717, 2274
PciI ACATGT 1 cut(s) 1380
PfeI GAWTC 6 cut(s) 544, 808, 998, 1004, 1341, 2815
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2500
PkrI GCNGC 5 cut(s) 172, 340, 541, 2713, 2724
PleI GAGTC 4 cut(s) 133, 1360, 2143, 2234
PpsI GAGTC 4 cut(s) 133, 1360, 2143, 2234
Ppu21I YACGTR 3 cut(s) 165, 1210, 1490
PpuMI RGGWCCY 1 cut(s) 2676
PscI ACATGT 1 cut(s) 1380
PshBI ATTAAT 2 cut(s) 369, 1203
PsiI TTATAA 1 cut(s) 1821
Psp5II RGGWCCY 1 cut(s) 2676
Psp6I CCWGG 4 cut(s) 216, 1391, 2316, 2500
PspEI GGTNACC 1 cut(s) 914
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 4 cut(s) 216, 1391, 2316, 2500
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 7 cut(s) 1432, 1675, 2382, 2499, 2677, 2678, 2778
PspPI GGNCC 6 cut(s) 223, 896, 1993, 2085, 2498, 2676
PspPPI RGGWCCY 1 cut(s) 2676
PstI CTGCAG 2 cut(s) 343, 2472
PsuI RGATCY 3 cut(s) 941, 2050, 2849
PvuII CAGCTG 1 cut(s) 437
RseI CAYNNNNRTG 4 cut(s) 534, 970, 1668, 2328
SatI GCNGC 5 cut(s) 171, 339, 540, 2712, 2723
Sau96I GGNCC 6 cut(s) 223, 896, 1993, 2085, 2498, 2676
ScaI AGTACT 1 cut(s) 2125
SchI GAGTC 4 cut(s) 133, 1361, 2144, 2235
ScrFI CCNGG 5 cut(s) 218, 1393, 1679, 2318, 2502
SduI GDGCHC 3 cut(s) 1392, 1433, 2779
SfaNI GCATC 7 cut(s) 430, 538, 682, 1042, 1463, 2064, 2846
SfcI CTRYAG 4 cut(s) 339, 444, 1988, 2468
SfiI GGCCNNNNNGGCC 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 745
SinI GGWCC 3 cut(s) 1993, 2498, 2676
SmiMI CAYNNNNRTG 4 cut(s) 534, 970, 1668, 2328
SmlI CTYRAG 7 cut(s) 239, 1520, 2080, 2534, 2615, 2632, 2681
SmoI CTYRAG 7 cut(s) 239, 1520, 2080, 2534, 2615, 2632, 2681
SpeI ACTAGT 1 cut(s) 1697
SseBI AGGCCT 2 cut(s) 717, 2274
SsiI CCGC 1 cut(s) 590
SspI AATATT 1 cut(s) 751
SspMI CTAG 4 cut(s) 261, 1698, 2021, 2855
StuI AGGCCT 2 cut(s) 717, 2274
StyD4I CCNGG 5 cut(s) 216, 1391, 1677, 2316, 2500
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 7 cut(s) 823, 1767, 1852, 1915, 1950, 2491, 2872
TaiI ACGT 7 cut(s) 167, 1104, 1212, 1492, 1641, 2509, 2543
TaqI TCGA 7 cut(s) 363, 595, 745, 2281, 2326, 2604, 2880
TaqII GACCGA 1 cut(s) 771
TatI WGTACW 5 cut(s) 51, 297, 566, 1767, 2123
TfiI GAWTC 6 cut(s) 544, 808, 998, 1004, 1341, 2815
TscAI CASTG 5 cut(s) 162, 193, 340, 2496, 2721
TseFI GTSAC 5 cut(s) 914, 1578, 1804, 1915, 2904
TseI GCWGC 5 cut(s) 170, 338, 539, 2711, 2722
Tsp45I GTSAC 5 cut(s) 914, 1578, 1804, 1915, 2904
TspGWI ACGGA 4 cut(s) 53, 702, 1523, 2450
TspRI CASTG 5 cut(s) 162, 193, 340, 2496, 2721
Vha464I CTTAAG 1 cut(s) 1520
VpaK11BI GGWCC 3 cut(s) 1993, 2498, 2676
VspI ATTAAT 2 cut(s) 369, 1203
XagI CCTNNNNNAGG 2 cut(s) 722, 2268
XceI RCATGY 2 cut(s) 1072, 1384
XcmI CCANNNNNNNNNTGG 1 cut(s) 1790
XspI CTAG 4 cut(s) 261, 1698, 2021, 2855
ZrmI AGTACT 1 cut(s) 2125
Zsp2I ATGCAT 2 cut(s) 675, 2839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.