Rroxscaffold_6G00399680

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001584
Physical Location & Seq
Reverse (-)
21701748 .. 21714819
13072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399680.1

Sequence Viewer

Length: 2736 bp
ATGGCTGTGGGGAAAGAAGAGATATTGAAAGCCCGTACGGACAGCAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATACTTACTCGAAGTACATCACTGAGCATGGAGGAAGAACAAATGCTTTCACAGCTGCAGAACACACCAACTTTTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGACGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGAATACTTTGGAGGTTCGACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATATGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGATATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGAGCACATGCGAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGCGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCAGTATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCAGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATGCAAACATGTTCGAGCCCTGGTATGAAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATGAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCAACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGCCCAGTTCTGTTAAGAAAGTCACCATATACAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATATGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGAAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTCAGGTTGCTGAACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTCTTGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCAATGATGCTCTCAAGGGCCTTCTTAGAGTGTTACACAGCAGAGGGATTTTGTTTTTCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTGCTTGTTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACATAACTGCTCTTTTACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGTTTCTTGGTTGTAGTTTTGACCCCGAGGGAAAAGGTGTGGAGTTTTTGGGTATAGTTGTATGCAGAATTGACCTTATTGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAGTCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

911

Amino Acids

104.82

Weight (kDa)

5.89

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 163 1.5e-36 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 191 - 368 3.9e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 376 - 619 7.6e-90 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 690 - 788 7.1e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1761, 2532
AccB1I GGYRCC 1 cut(s) 2554
AccII CGCG 1 cut(s) 2169
AciI CCGC 2 cut(s) 2167, 2169
AclI AACGTT 1 cut(s) 2246
AclWI GGATC 4 cut(s) 68, 95, 901, 2011
AcoI YGGCCR 2 cut(s) 877, 1859
AcuI CTGAAG 5 cut(s) 458, 1222, 1641, 2022, 2045
AfaI GTAC 9 cut(s) 37, 53, 224, 251, 520, 1541, 1709, 1999, 2580
AfiI CCNNNNNNNGG 5 cut(s) 232, 456, 676, 841, 2696
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 1 cut(s) 1332
AjnI CCWGG 3 cut(s) 168, 1343, 2206
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 3 cut(s) 796, 1112, 1473
AlwI GGATC 4 cut(s) 68, 95, 901, 2011
Ama87I CYCGRG 1 cut(s) 2435
AoxI GGCC 9 cut(s) 166, 175, 667, 812, 848, 877, 1009, 1859, 1941
ApeKI GCWGC 5 cut(s) 122, 290, 491, 2065, 2500
AseI ATTAAT 2 cut(s) 321, 1155
Asp700I GAANNNNTTC 1 cut(s) 1884
AspS9I GGNCC 6 cut(s) 175, 848, 1849, 1941, 2204, 2382
AsuHPI GGTGA 7 cut(s) 743, 824, 959, 991, 1334, 1512, 1756
AsuII TTCGAA 1 cut(s) 697
AvaI CYCGRG 1 cut(s) 2435
AvaII GGWCC 3 cut(s) 1849, 2204, 2382
BaeGI GKGCMC 1 cut(s) 2557
BalI TGGCCA 1 cut(s) 1861
BanI GGYRCC 1 cut(s) 2554
BanII GRGCYC 2 cut(s) 1344, 1385
BauI CACGAG 1 cut(s) 1013
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 5 cut(s) 109, 277, 503, 2052, 2512
BccI CCATC 6 cut(s) 158, 323, 446, 918, 1861, 2372
BcgI CGANNNNNNTGC 2 cut(s) 2012, 2046
BciT130I CCWGG 3 cut(s) 170, 1345, 2208
BciVI GTATCC 3 cut(s) 229, 562, 2174
BclI TGATCA 1 cut(s) 1842
BcoDI GTCTC 3 cut(s) 796, 1112, 1473
BfaI CTAG 2 cut(s) 213, 1877
BfmI CTRYAG 2 cut(s) 291, 2174
BfrI CTTAAG 1 cut(s) 1472
BfuI GTATCC 3 cut(s) 229, 562, 2174
BglI GCCNNNNNGGC 1 cut(s) 174
BglII AGATCT 1 cut(s) 1906
BisI GCNGC 5 cut(s) 123, 291, 492, 2066, 2501
BlsI GCNGC 5 cut(s) 124, 292, 493, 2067, 2502
BmcAI AGTACT 1 cut(s) 1999
Bme1390I CCNGG 3 cut(s) 170, 1345, 2208
Bme18I GGWCC 3 cut(s) 1849, 2204, 2382
BmeT110I CYCGRG 1 cut(s) 2435
BmgT120I GGNCC 6 cut(s) 175, 848, 1849, 1941, 2204, 2382
BmiI GGNNCC 6 cut(s) 1384, 1615, 2205, 2383, 2384, 2556
BmrFI CCNGG 3 cut(s) 170, 1345, 2208
BmrI ACTGGG 2 cut(s) 1496, 2627
BmsI GCATC 7 cut(s) 490, 634, 994, 1315, 1415, 1920, 2624
BmuI ACTGGG 2 cut(s) 1496, 2627
BpmI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
Bpu10I CCTNAGC 1 cut(s) 1692
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 5 cut(s) 212, 1921, 2342, 2359, 2372
Bsa29I ATCGAT 1 cut(s) 2310
BsaAI YACGTR 2 cut(s) 1162, 1442
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 4 cut(s) 129, 1343, 2167, 2436
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 456, 676, 841, 2696
Bse1I ACTGG 5 cut(s) 733, 1211, 1502, 1788, 2633
Bse3DI GCAATG 2 cut(s) 1223, 2299
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 3 cut(s) 170, 1345, 2208
BseCI ATCGAT 1 cut(s) 2310
BseDI CCNNGG 4 cut(s) 129, 1343, 2167, 2436
BseGI GGATG 4 cut(s) 139, 499, 929, 1452
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 5 cut(s) 232, 456, 676, 841, 2696
BseMI GCAATG 2 cut(s) 1223, 2299
BseMII CTCAG 6 cut(s) 249, 374, 1706, 1998, 2486, 2598
BseNI ACTGG 5 cut(s) 733, 1211, 1502, 1788, 2633
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2557
BseXI GCAGC 5 cut(s) 109, 277, 503, 2052, 2512
BsgI GTGCAG 2 cut(s) 510, 2006
Bsh1236I CGCG 1 cut(s) 2169
BshFI GGCC 9 cut(s) 168, 177, 669, 814, 850, 879, 1011, 1861, 1943
BshNI GGYRCC 1 cut(s) 2554
BshVI ATCGAT 1 cut(s) 2310
BsiHKAI GWGCWC 1 cut(s) 1020
BsiHKCI CYCGRG 1 cut(s) 2435
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 761, 1584, 2395
BslI CCNNNNNNNGG 5 cut(s) 232, 456, 676, 841, 2696
BsmAI GTCTC 3 cut(s) 796, 1112, 1473
BsmFI GGGAC 3 cut(s) 761, 1584, 2395
BsnI GGCC 9 cut(s) 168, 177, 669, 814, 850, 879, 1011, 1861, 1943
BsoBI CYCGRG 1 cut(s) 2435
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 4 cut(s) 1020, 1344, 1385, 2557
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 2 cut(s) 2167, 2169
BspANI GGCC 9 cut(s) 168, 177, 669, 814, 850, 879, 1011, 1861, 1943
BspCNI CTCAG 6 cut(s) 250, 373, 1705, 1999, 2487, 2599
BspDI ATCGAT 1 cut(s) 2310
BspFNI CGCG 1 cut(s) 2169
BspLI GGNNCC 6 cut(s) 1384, 1615, 2205, 2383, 2384, 2556
BspMAI CTGCAG 2 cut(s) 295, 2178
BspPI GGATC 4 cut(s) 68, 95, 901, 2011
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 1 cut(s) 2554
BspTI CTTAAG 1 cut(s) 1472
BsrDI GCAATG 2 cut(s) 1223, 2299
BsrI ACTGG 5 cut(s) 733, 1211, 1502, 1788, 2633
BssECI CCNNGG 4 cut(s) 129, 1343, 2167, 2436
BssSI CACGAG 1 cut(s) 1013
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 1013
Bst2UI CCWGG 3 cut(s) 170, 1345, 2208
Bst4CI ACNGT 5 cut(s) 775, 1707, 1792, 2197, 2650
Bst6I CTCTTC 2 cut(s) 12, 333
BstAFI CTTAAG 1 cut(s) 1472
BstBAI YACGTR 2 cut(s) 1162, 1442
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 3 cut(s) 1009, 1040, 1810
BstDSI CCRYGG 2 cut(s) 129, 2167
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 4 cut(s) 139, 499, 929, 1452
BstFNI CGCG 1 cut(s) 2169
BstMAI GTCTC 3 cut(s) 796, 1112, 1473
BstMWI GCNNNNNNNGC 7 cut(s) 174, 287, 478, 1270, 1688, 1697, 2326
BstNI CCWGG 3 cut(s) 170, 1345, 2208
BstNSI RCATGY 2 cut(s) 1024, 1336
BstSCI CCNGG 3 cut(s) 168, 1343, 2206
BstSFI CTRYAG 2 cut(s) 291, 2174
BstSLI GKGCMC 1 cut(s) 2557
BstUI CGCG 1 cut(s) 2169
BstV1I GCAGC 5 cut(s) 109, 277, 503, 2052, 2512
BstX2I RGATCY 2 cut(s) 893, 1906
BstXI CCANNNNNNTGG 1 cut(s) 1610
BstYI RGATCY 2 cut(s) 893, 1906
Bsu15I ATCGAT 1 cut(s) 2310
BsuI GTATCC 3 cut(s) 229, 562, 2174
BsuRI GGCC 9 cut(s) 168, 177, 669, 814, 850, 879, 1011, 1861, 1943
BsuTUI ATCGAT 1 cut(s) 2310
BtgI CCRYGG 2 cut(s) 129, 2167
BtsCI GGATG 4 cut(s) 139, 499, 929, 1452
BtsIMutI CAGTG 6 cut(s) 145, 255, 2202, 2492, 2640, 2646
Cac8I GCNNGC 3 cut(s) 1009, 1040, 1810
Cfr13I GGNCC 6 cut(s) 175, 848, 1849, 1941, 2204, 2382
Cfr42I CCGCGG 1 cut(s) 2170
ClaI ATCGAT 1 cut(s) 2310
CseI GACGC 1 cut(s) 401
Csp6I GTAC 9 cut(s) 36, 52, 223, 250, 519, 1540, 1708, 1998, 2579
CviQI GTAC 9 cut(s) 36, 52, 223, 250, 519, 1540, 1708, 1998, 2579
DraI TTTAAA 2 cut(s) 982, 1816
EaeI YGGCCR 2 cut(s) 877, 1859
Eam1104I CTCTTC 2 cut(s) 12, 333
EarI CTCTTC 2 cut(s) 12, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 669
Eco24I GRGCYC 2 cut(s) 1344, 1385
Eco32I GATATC 1 cut(s) 860
Eco47I GGWCC 3 cut(s) 1849, 2204, 2382
Eco57I CTGAAG 5 cut(s) 458, 1222, 1641, 2022, 2045
Eco88I CYCGRG 1 cut(s) 2435
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO109I RGGNCCY 3 cut(s) 175, 1941, 2382
EcoRI GAATTC 2 cut(s) 1389, 2282
EcoRII CCWGG 3 cut(s) 168, 1343, 2206
EcoRV GATATC 1 cut(s) 860
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 627, 2617
EcoT38I GRGCYC 2 cut(s) 1344, 1385
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 4 cut(s) 636, 668, 2699, 2731
FaqI GGGAC 3 cut(s) 761, 1584, 2395
FauNDI CATATG 2 cut(s) 198, 1576
FbaI TGATCA 1 cut(s) 1842
Fnu4HI GCNGC 5 cut(s) 123, 291, 492, 2066, 2501
FokI GGATG 4 cut(s) 146, 486, 936, 1439
FriOI GRGCYC 2 cut(s) 1344, 1385
Fsp4HI GCNGC 5 cut(s) 123, 291, 492, 2066, 2501
FspBI CTAG 2 cut(s) 213, 1877
GluI GCNGC 5 cut(s) 123, 291, 492, 2066, 2501
GsuI CTGGAG 5 cut(s) 191, 478, 776, 807, 1369
HaeIII GGCC 9 cut(s) 168, 177, 669, 814, 850, 879, 1011, 1861, 1943
HgaI GACGC 1 cut(s) 401
HindIII AAGCTT 4 cut(s) 1271, 1810, 2258, 2318
HphI GGTGA 7 cut(s) 743, 824, 959, 991, 1334, 1512, 1756
Hpy166II GTNNAC 1 cut(s) 2579
Hpy8I GTNNAC 1 cut(s) 2579
HpyCH4III ACNGT 5 cut(s) 775, 1707, 1792, 2197, 2650
HpyCH4IV ACGT 4 cut(s) 1161, 1441, 2025, 2246
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 287, 478, 1270, 1688, 1697, 2326
HpySE526I ACGT 4 cut(s) 1161, 1441, 2025, 2246
KflI GGGWCCC 1 cut(s) 2382
Ksp22I TGATCA 1 cut(s) 1842
KspI CCGCGG 1 cut(s) 2170
LmnI GCTCC 3 cut(s) 1388, 1619, 2633
Lsp1109I GCAGC 5 cut(s) 109, 277, 503, 2052, 2512
LweI GCATC 7 cut(s) 490, 634, 994, 1315, 1415, 1920, 2624
MaeI CTAG 2 cut(s) 213, 1877
MaeII ACGT 4 cut(s) 1161, 1441, 2025, 2246
MaeIII GTNAC 6 cut(s) 1062, 1518, 1744, 1955, 2126, 2682
MfeI CAATTG 2 cut(s) 526, 1594
MflI RGATCY 2 cut(s) 893, 1906
MhlI GDGCHC 4 cut(s) 1020, 1344, 1385, 2557
MlsI TGGCCA 1 cut(s) 1861
MluNI TGGCCA 1 cut(s) 1861
MlyI GAGTC 3 cut(s) 1313, 1741, 2018
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 1 cut(s) 1861
Mph1103I ATGCAT 2 cut(s) 627, 2617
MroXI GAANNNNTTC 1 cut(s) 1884
MscI TGGCCA 1 cut(s) 1861
MslI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2034
Msp20I TGGCCA 1 cut(s) 1861
MspA1I CMGCKG 4 cut(s) 290, 389, 2065, 2169
MspCI CTTAAG 1 cut(s) 1472
MspR9I CCNGG 3 cut(s) 170, 1345, 2208
MunI CAATTG 2 cut(s) 526, 1594
MvaI CCWGG 3 cut(s) 170, 1345, 2208
MvnI CGCG 1 cut(s) 2169
MwoI GCNNNNNNNGC 7 cut(s) 174, 287, 478, 1270, 1688, 1697, 2326
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 198, 1576
NlaIV GGNNCC 6 cut(s) 1384, 1615, 2205, 2383, 2384, 2556
NmuCI GTSAC 3 cut(s) 1518, 1744, 2682
NsiI ATGCAT 2 cut(s) 627, 2617
NspI RCATGY 2 cut(s) 1024, 1336
NspV TTCGAA 1 cut(s) 697
PceI AGGCCT 1 cut(s) 669
PciI ACATGT 1 cut(s) 1332
PdmI GAANNNNTTC 1 cut(s) 1884
PfeI GAWTC 7 cut(s) 422, 496, 760, 950, 956, 1293, 2593
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2206
PkrI GCNGC 5 cut(s) 124, 292, 493, 2067, 2502
PleI GAGTC 3 cut(s) 1312, 1741, 2017
PpsI GAGTC 3 cut(s) 1312, 1741, 2017
Ppu21I YACGTR 2 cut(s) 1162, 1442
PpuMI RGGWCCY 1 cut(s) 2382
PscI ACATGT 1 cut(s) 1332
PshBI ATTAAT 2 cut(s) 321, 1155
PsiI TTATAA 2 cut(s) 1761, 2532
Psp1406I AACGTT 1 cut(s) 2246
Psp5II RGGWCCY 1 cut(s) 2382
Psp6I CCWGG 3 cut(s) 168, 1343, 2206
PspGI CCWGG 3 cut(s) 168, 1343, 2206
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1384, 1615, 2205, 2383, 2384, 2556
PspPI GGNCC 6 cut(s) 175, 848, 1849, 1941, 2204, 2382
PspPPI RGGWCCY 1 cut(s) 2382
PstI CTGCAG 2 cut(s) 295, 2178
PsuI RGATCY 2 cut(s) 893, 1906
PvuII CAGCTG 3 cut(s) 290, 389, 2065
RsaI GTAC 9 cut(s) 37, 53, 224, 251, 520, 1541, 1709, 1999, 2580
RsaNI GTAC 9 cut(s) 36, 52, 223, 250, 519, 1540, 1708, 1998, 2579
RseI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2034
SacII CCGCGG 1 cut(s) 2170
SatI GCNGC 5 cut(s) 123, 291, 492, 2066, 2501
Sau96I GGNCC 6 cut(s) 175, 848, 1849, 1941, 2204, 2382
ScaI AGTACT 1 cut(s) 1999
SchI GAGTC 3 cut(s) 1313, 1741, 2018
ScrFI CCNGG 3 cut(s) 170, 1345, 2208
SduI GDGCHC 4 cut(s) 1020, 1344, 1385, 2557
SfaNI GCATC 7 cut(s) 490, 634, 994, 1315, 1415, 1920, 2624
SfcI CTRYAG 2 cut(s) 291, 2174
SfiI GGCCNNNNNGGCC 1 cut(s) 174
Sfr303I CCGCGG 1 cut(s) 2170
SfuI TTCGAA 1 cut(s) 697
SgrBI CCGCGG 1 cut(s) 2170
SinI GGWCC 3 cut(s) 1849, 2204, 2382
SmiMI CAYNNNNRTG 4 cut(s) 486, 922, 1608, 2034
SmlI CTYRAG 6 cut(s) 191, 1472, 1936, 2321, 2338, 2387
SmoI CTYRAG 6 cut(s) 191, 1472, 1936, 2321, 2338, 2387
SseBI AGGCCT 1 cut(s) 669
SsiI CCGC 2 cut(s) 2167, 2169
SspI AATATT 1 cut(s) 1642
SspMI CTAG 2 cut(s) 213, 1877
StuI AGGCCT 1 cut(s) 669
StyD4I CCNGG 3 cut(s) 168, 1343, 2206
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 5 cut(s) 775, 1707, 1792, 2197, 2650
TaiI ACGT 4 cut(s) 1164, 1444, 2028, 2249
TaqI TCGA 7 cut(s) 245, 547, 697, 1338, 2032, 2310, 2658
TaqII GACCGA 1 cut(s) 723
TatI WGTACW 5 cut(s) 51, 249, 518, 1707, 1997
TfiI GAWTC 7 cut(s) 422, 496, 760, 950, 956, 1293, 2593
TscAI CASTG 6 cut(s) 145, 262, 2202, 2499, 2640, 2653
TseFI GTSAC 3 cut(s) 1518, 1744, 2682
TseI GCWGC 5 cut(s) 122, 290, 491, 2065, 2500
Tsp45I GTSAC 3 cut(s) 1518, 1744, 2682
TspGWI ACGGA 2 cut(s) 53, 1475
TspRI CASTG 6 cut(s) 145, 262, 2202, 2499, 2640, 2653
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 1849, 2204, 2382
VspI ATTAAT 2 cut(s) 321, 1155
XagI CCTNNNNNAGG 1 cut(s) 674
XceI RCATGY 2 cut(s) 1024, 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1730
XmnI GAANNNNTTC 1 cut(s) 1884
XspI CTAG 2 cut(s) 213, 1877
ZrmI AGTACT 1 cut(s) 1999
Zsp2I ATGCAT 2 cut(s) 627, 2617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.