Rw3G021870

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001584
Physical Location & Seq
Reverse (-)
26576010 .. 26593971
17962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G021870.1

Sequence Viewer

Length: 2895 bp
ATGACGCTTTCAAAGCGCCTCAATCAATGGAGTTTGAGCAAAGAGATGTATGTTCTCAATGTGGAGTGTCTAATCATTGGGCACACATTTGTAGAGCTCATGAAGAAATCGTCACCGCCTACAAAGCATATTGGCACCGCCTTCATCCACCACCAGCTTGGGGTTGGCGCAGTCCTTATCCATGACGCCATGGATGGCGTCCATCATGGTGATGGCGCCCTAGCCTCTCGCTCTTTTTACAATGCCCGTTCCTTAGGGAAATTAGGACTGAGACCGTCCTATGCAAAGGATATGAAAATACTCATTATTTATGCTAATTCGTCTACGACCCACTGCCACTCAATCTATCTCTGCGTTATAGCTAGTGACTGGGTACAAGTATTATACTTACGCATATTTGAGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGAATACTTTGGAGGTTCAACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTATTTGTGAGACAAAGTTTCATTATCAGGACAAAATTGAGCCTCTTAATTACGTGATCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTTGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGTCATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATGAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTGATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTAGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGCAGTACCAAAATTACAAATTTCAACAGCCTTATGGGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGGAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCATGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCGACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCGAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTACGCTGAATGTGAAACTTGAGCTGTTTGGTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACATAACTGCTCTTAAACCGAGGAATGATTATGGTATCTGTGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGTGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGCTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

964

Amino Acids

110.95

Weight (kDa)

7.09

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 190 - 367 1.2e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 375 - 654 3.6e-104 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 659 - 845 7.3e-14 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 769 - 865 2.7e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1758, 2691
AccB1I GGYRCC 3 cut(s) 134, 215, 2713
AccI GTMKAC 1 cut(s) 323
AciI CCGC 2 cut(s) 116, 138
AclI AACGTT 1 cut(s) 2477
AclWI GGATC 2 cut(s) 898, 2074
AcoI YGGCCR 2 cut(s) 874, 1940
AcuI CTGAAG 3 cut(s) 455, 1219, 2085
AcyI GRCGYC 3 cut(s) 186, 198, 216
AfaI GTAC 8 cut(s) 375, 517, 1538, 1706, 1865, 1907, 2062, 2739
AfiI CCNNNNNNNGG 6 cut(s) 160, 453, 673, 838, 1346, 2207
AflII CTTAAG 1 cut(s) 1469
AflIII ACRYGT 1 cut(s) 1329
AhlI ACTAGT 1 cut(s) 1634
AjnI CCWGG 3 cut(s) 1340, 2253, 2437
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 4 cut(s) 265, 793, 1109, 1470
AlwI GGATC 2 cut(s) 898, 2074
AoxI GGCC 8 cut(s) 664, 809, 845, 874, 1006, 1940, 2022, 2209
ApeKI GCWGC 3 cut(s) 488, 2648, 2659
AseI ATTAAT 1 cut(s) 1919
Asp700I GAANNNNTTC 1 cut(s) 1965
AspLEI GCGC 3 cut(s) 18, 170, 218
AspS9I GGNCC 5 cut(s) 845, 1930, 2022, 2435, 2613
AsuC2I CCSGG 1 cut(s) 1616
AsuHPI GGTGA 9 cut(s) 105, 221, 740, 821, 956, 988, 1331, 1509, 1753
AsuII TTCGAA 1 cut(s) 694
AvaII GGWCC 3 cut(s) 1930, 2435, 2613
AxyI CCTNAGG 1 cut(s) 253
BaeGI GKGCMC 2 cut(s) 84, 2716
BalI TGGCCA 1 cut(s) 1942
BanI GGYRCC 3 cut(s) 134, 215, 2713
BanII GRGCYC 3 cut(s) 99, 1341, 1382
Bbv12I GWGCWC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 1689
BbvI GCAGC 3 cut(s) 500, 2660, 2671
BccI CCATC 8 cut(s) 188, 206, 210, 443, 915, 1942, 2149, 2603
BciT130I CCWGG 3 cut(s) 1342, 2255, 2439
BciVI GTATCC 1 cut(s) 559
BclI TGATCA 2 cut(s) 1161, 1839
BcnI CCSGG 1 cut(s) 1616
BcoDI GTCTC 4 cut(s) 265, 793, 1109, 1470
BcuI ACTAGT 1 cut(s) 1634
BfaI CTAG 5 cut(s) 221, 363, 1635, 1781, 1958
BfmI CTRYAG 2 cut(s) 1925, 2405
BfoI RGCGCY 2 cut(s) 19, 219
BfrI CTTAAG 1 cut(s) 1469
BfuI GTATCC 1 cut(s) 559
BglI GCCNNNNNGGC 1 cut(s) 1894
BglII AGATCT 1 cut(s) 1987
BisI GCNGC 3 cut(s) 489, 2649, 2660
BlsI GCNGC 3 cut(s) 490, 2650, 2661
BmcAI AGTACT 1 cut(s) 2062
Bme1390I CCNGG 4 cut(s) 1342, 1616, 2255, 2439
Bme18I GGWCC 3 cut(s) 1930, 2435, 2613
BmgT120I GGNCC 5 cut(s) 845, 1930, 2022, 2435, 2613
BmiI GGNNCC 9 cut(s) 136, 217, 1381, 1612, 2051, 2436, 2614, 2615, 2715
BmrFI CCNGG 4 cut(s) 1342, 1616, 2255, 2439
BmrI ACTGGG 2 cut(s) 379, 2786
BmsI GCATC 6 cut(s) 487, 631, 991, 1412, 2001, 2783
BmuI ACTGGG 2 cut(s) 379, 2786
BpmI CTGGAG 4 cut(s) 475, 773, 804, 1366
Bpu10I CCTNAGC 1 cut(s) 1689
Bpu14I TTCGAA 1 cut(s) 694
BpuEI CTTGAG 5 cut(s) 2002, 2312, 2573, 2590, 2603
BpuMI CCSGG 1 cut(s) 1616
Bsa29I ATCGAT 2 cut(s) 2541, 2817
BsaAI YACGTR 2 cut(s) 1159, 1439
BsaBI GATNNNNATC 1 cut(s) 990
BsaHI GRCGYC 3 cut(s) 186, 198, 216
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 5 cut(s) 189, 1340, 1455, 1614, 2378
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 453, 673, 838, 1346, 2207
Bse1I ACTGG 6 cut(s) 374, 730, 1208, 1241, 1499, 2792
Bse21I CCTNAGG 1 cut(s) 253
Bse3DI GCAATG 2 cut(s) 1220, 2530
Bse8I GATNNNNATC 1 cut(s) 990
BseBI CCWGG 3 cut(s) 1342, 2255, 2439
BseCI ATCGAT 2 cut(s) 2541, 2817
BseDI CCNNGG 5 cut(s) 189, 1340, 1455, 1614, 2378
BseGI GGATG 6 cut(s) 144, 199, 496, 926, 1449, 2141
BseJI GATNNNNATC 1 cut(s) 990
BseLI CCNNNNNNNGG 6 cut(s) 160, 453, 673, 838, 1346, 2207
BseMI GCAATG 2 cut(s) 1220, 2530
BseMII CTCAG 5 cut(s) 260, 1680, 2061, 2645, 2757
BseNI ACTGG 6 cut(s) 374, 730, 1208, 1241, 1499, 2792
BseSI GKGCMC 2 cut(s) 84, 2716
BseXI GCAGC 3 cut(s) 500, 2660, 2671
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 8 cut(s) 666, 811, 847, 876, 1008, 1942, 2024, 2211
BshNI GGYRCC 3 cut(s) 134, 215, 2713
BshVI ATCGAT 2 cut(s) 2541, 2817
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 1616
BslFI GGGAC 3 cut(s) 758, 1581, 2626
BslI CCNNNNNNNGG 6 cut(s) 160, 453, 673, 838, 1346, 2207
BsmAI GTCTC 4 cut(s) 265, 793, 1109, 1470
BsmFI GGGAC 3 cut(s) 758, 1581, 2626
BsnI GGCC 8 cut(s) 666, 811, 847, 876, 1008, 1942, 2024, 2211
Bso31I GGTCTC 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 694
Bsp1286I GDGCHC 5 cut(s) 84, 99, 1341, 1382, 2716
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 2 cut(s) 116, 138
BspANI GGCC 8 cut(s) 666, 811, 847, 876, 1008, 1942, 2024, 2211
BspCNI CTCAG 5 cut(s) 261, 1681, 2062, 2646, 2758
BspDI ATCGAT 2 cut(s) 2541, 2817
BspHI TCATGA 1 cut(s) 99
BspLI GGNNCC 9 cut(s) 136, 217, 1381, 1612, 2051, 2436, 2614, 2615, 2715
BspMAI CTGCAG 1 cut(s) 2409
BspPI GGATC 2 cut(s) 898, 2074
BspT104I TTCGAA 1 cut(s) 694
BspT107I GGYRCC 3 cut(s) 134, 215, 2713
BspTI CTTAAG 1 cut(s) 1469
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 2 cut(s) 1220, 2530
BsrI ACTGG 6 cut(s) 374, 730, 1208, 1241, 1499, 2792
BssECI CCNNGG 5 cut(s) 189, 1340, 1455, 1614, 2378
BssNI GRCGYC 3 cut(s) 186, 198, 216
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 1342, 2255, 2439
Bst4CI ACNGT 6 cut(s) 276, 772, 1704, 1789, 2428, 2809
BstACI GRCGYC 3 cut(s) 186, 198, 216
BstAFI CTTAAG 1 cut(s) 1469
BstAPI GCANNNNNTGC 1 cut(s) 2645
BstBAI YACGTR 2 cut(s) 1159, 1439
BstBI TTCGAA 1 cut(s) 694
BstC8I GCNNGC 4 cut(s) 1006, 1037, 1807, 1899
BstDSI CCRYGG 2 cut(s) 189, 1455
BstENI CCTNNNNNAGG 2 cut(s) 671, 2205
BstF5I GGATG 6 cut(s) 144, 199, 496, 926, 1449, 2141
BstH2I RGCGCY 2 cut(s) 19, 219
BstHHI GCGC 3 cut(s) 18, 170, 218
BstMAI GTCTC 4 cut(s) 265, 793, 1109, 1470
BstMWI GCNNNNNNNGC 9 cut(s) 13, 124, 475, 1267, 1685, 1694, 1894, 2557, 2645
BstNI CCWGG 3 cut(s) 1342, 2255, 2439
BstNSI RCATGY 3 cut(s) 1021, 1333, 2090
BstSCI CCNGG 4 cut(s) 1340, 1614, 2253, 2437
BstSFI CTRYAG 2 cut(s) 1925, 2405
BstSLI GKGCMC 2 cut(s) 84, 2716
BstV1I GCAGC 3 cut(s) 500, 2660, 2671
BstX2I RGATCY 2 cut(s) 890, 1987
BstXI CCANNNNNNTGG 2 cut(s) 158, 1607
BstYI RGATCY 2 cut(s) 890, 1987
Bsu15I ATCGAT 2 cut(s) 2541, 2817
Bsu36I CCTNAGG 1 cut(s) 253
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 8 cut(s) 666, 811, 847, 876, 1008, 1942, 2024, 2211
BsuTUI ATCGAT 2 cut(s) 2541, 2817
BtgI CCRYGG 2 cut(s) 189, 1455
BtsCI GGATG 6 cut(s) 144, 199, 496, 926, 1449, 2141
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 5 cut(s) 331, 2433, 2651, 2799, 2805
Cac8I GCNNGC 4 cut(s) 1006, 1037, 1807, 1899
CciI TCATGA 1 cut(s) 99
CfoI GCGC 3 cut(s) 18, 170, 218
Cfr13I GGNCC 5 cut(s) 845, 1930, 2022, 2435, 2613
ClaI ATCGAT 2 cut(s) 2541, 2817
CseI GACGC 3 cut(s) 13, 187, 194
Csp6I GTAC 8 cut(s) 374, 516, 1537, 1705, 1864, 1906, 2061, 2738
CviQI GTAC 8 cut(s) 374, 516, 1537, 1705, 1864, 1906, 2061, 2738
DinI GGCGCC 1 cut(s) 217
DraI TTTAAA 2 cut(s) 979, 1813
EaeI YGGCCR 2 cut(s) 874, 1940
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 2 cut(s) 666, 2211
Eco24I GRGCYC 3 cut(s) 99, 1341, 1382
Eco31I GGTCTC 1 cut(s) 265
Eco47I GGWCC 3 cut(s) 1930, 2435, 2613
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 3 cut(s) 455, 1219, 2085
Eco81I CCTNAGG 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 2 cut(s) 671, 2205
EcoO109I RGGNCCY 2 cut(s) 2022, 2613
EcoRI GAATTC 3 cut(s) 1386, 2230, 2513
EcoRII CCWGG 3 cut(s) 1340, 2253, 2437
EcoT14I CCWWGG 1 cut(s) 189
EcoT22I ATGCAT 2 cut(s) 624, 2776
EcoT38I GRGCYC 3 cut(s) 99, 1341, 1382
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 189
FalI AAGNNNNNCTT 6 cut(s) 633, 665, 1191, 1223, 2858, 2890
FaqI GGGAC 3 cut(s) 758, 1581, 2626
FauNDI CATATG 1 cut(s) 1522
FbaI TGATCA 2 cut(s) 1161, 1839
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 3 cut(s) 489, 2649, 2660
FokI GGATG 6 cut(s) 131, 206, 483, 933, 1436, 2128
FriOI GRGCYC 3 cut(s) 99, 1341, 1382
Fsp4HI GCNGC 3 cut(s) 489, 2649, 2660
FspBI CTAG 5 cut(s) 221, 363, 1635, 1781, 1958
GlaI GCGC 3 cut(s) 17, 169, 217
GluI GCNGC 3 cut(s) 489, 2649, 2660
GsuI CTGGAG 4 cut(s) 475, 773, 804, 1366
HaeII RGCGCY 2 cut(s) 19, 219
HaeIII GGCC 8 cut(s) 666, 811, 847, 876, 1008, 1942, 2024, 2211
HapII CCGG 1 cut(s) 1616
HgaI GACGC 3 cut(s) 13, 187, 194
HhaI GCGC 3 cut(s) 18, 170, 218
Hin1I GRCGYC 3 cut(s) 186, 198, 216
Hin6I GCGC 3 cut(s) 16, 168, 216
HinP1I GCGC 3 cut(s) 16, 168, 216
HindIII AAGCTT 5 cut(s) 1268, 1807, 2171, 2489, 2549
HpaII CCGG 1 cut(s) 1616
HphI GGTGA 9 cut(s) 105, 221, 740, 821, 956, 988, 1331, 1509, 1753
Hpy166II GTNNAC 4 cut(s) 324, 2245, 2402, 2738
Hpy8I GTNNAC 4 cut(s) 324, 2245, 2402, 2738
HpyCH4III ACNGT 6 cut(s) 276, 772, 1704, 1789, 2428, 2809
HpyCH4IV ACGT 4 cut(s) 1158, 1438, 1575, 2477
HpyF10VI GCNNNNNNNGC 9 cut(s) 13, 124, 475, 1267, 1685, 1694, 1894, 2557, 2645
HpySE526I ACGT 4 cut(s) 1158, 1438, 1575, 2477
Hsp92I GRCGYC 3 cut(s) 186, 198, 216
HspAI GCGC 3 cut(s) 16, 168, 216
KasI GGCGCC 1 cut(s) 215
KflI GGGWCCC 1 cut(s) 2613
Ksp22I TGATCA 2 cut(s) 1161, 1839
LmnI GCTCC 3 cut(s) 1385, 1616, 2792
Lsp1109I GCAGC 3 cut(s) 500, 2660, 2671
LweI GCATC 6 cut(s) 487, 631, 991, 1412, 2001, 2783
MaeI CTAG 5 cut(s) 221, 363, 1635, 1781, 1958
MaeII ACGT 4 cut(s) 1158, 1438, 1575, 2477
MaeIII GTNAC 9 cut(s) 111, 365, 1059, 1515, 1741, 1852, 2036, 2357, 2841
MfeI CAATTG 2 cut(s) 523, 1591
MflI RGATCY 2 cut(s) 890, 1987
MhlI GDGCHC 5 cut(s) 84, 99, 1341, 1382, 2716
MlsI TGGCCA 1 cut(s) 1942
MluNI TGGCCA 1 cut(s) 1942
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 3 cut(s) 1310, 2081, 2172
MmeI TCCRAC 1 cut(s) 543
Mox20I TGGCCA 1 cut(s) 1942
Mph1103I ATGCAT 2 cut(s) 624, 2776
MroXI GAANNNNTTC 1 cut(s) 1965
MscI TGGCCA 1 cut(s) 1942
MslI CAYNNNNRTG 6 cut(s) 207, 210, 483, 919, 1605, 2265
Msp20I TGGCCA 1 cut(s) 1942
MspCI CTTAAG 1 cut(s) 1469
MspI CCGG 1 cut(s) 1616
MspR9I CCNGG 4 cut(s) 1342, 1616, 2255, 2439
MunI CAATTG 2 cut(s) 523, 1591
MvaI CCWGG 3 cut(s) 1342, 2255, 2439
MwoI GCNNNNNNNGC 9 cut(s) 13, 124, 475, 1267, 1685, 1694, 1894, 2557, 2645
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 1 cut(s) 1616
NcoI CCATGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 1522
NlaIV GGNNCC 9 cut(s) 136, 217, 1381, 1612, 2051, 2436, 2614, 2615, 2715
NmuCI GTSAC 6 cut(s) 111, 365, 1515, 1741, 1852, 2841
NsiI ATGCAT 2 cut(s) 624, 2776
NspI RCATGY 3 cut(s) 1021, 1333, 2090
NspV TTCGAA 1 cut(s) 694
PagI TCATGA 1 cut(s) 99
PceI AGGCCT 2 cut(s) 666, 2211
PciI ACATGT 1 cut(s) 1329
PdmI GAANNNNTTC 1 cut(s) 1965
PfeI GAWTC 7 cut(s) 419, 493, 757, 947, 953, 1290, 2752
PfoI TCCNGGA 1 cut(s) 2437
PkrI GCNGC 3 cut(s) 490, 2650, 2661
PleI GAGTC 3 cut(s) 1309, 2080, 2171
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 3 cut(s) 1309, 2080, 2171
Ppu21I YACGTR 2 cut(s) 1159, 1439
PpuMI RGGWCCY 1 cut(s) 2613
PscI ACATGT 1 cut(s) 1329
PshBI ATTAAT 1 cut(s) 1919
PsiI TTATAA 2 cut(s) 1758, 2691
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 2477
Psp5II RGGWCCY 1 cut(s) 2613
Psp6I CCWGG 3 cut(s) 1340, 2253, 2437
PspGI CCWGG 3 cut(s) 1340, 2253, 2437
PspN4I GGNNCC 9 cut(s) 136, 217, 1381, 1612, 2051, 2436, 2614, 2615, 2715
PspPI GGNCC 5 cut(s) 845, 1930, 2022, 2435, 2613
PspPPI RGGWCCY 1 cut(s) 2613
PstI CTGCAG 1 cut(s) 2409
PsuI RGATCY 2 cut(s) 890, 1987
RsaI GTAC 8 cut(s) 375, 517, 1538, 1706, 1865, 1907, 2062, 2739
RsaNI GTAC 8 cut(s) 374, 516, 1537, 1705, 1864, 1906, 2061, 2738
RseI CAYNNNNRTG 6 cut(s) 207, 210, 483, 919, 1605, 2265
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 3 cut(s) 489, 2649, 2660
Sau96I GGNCC 5 cut(s) 845, 1930, 2022, 2435, 2613
ScaI AGTACT 1 cut(s) 2062
SchI GAGTC 3 cut(s) 1310, 2081, 2172
ScrFI CCNGG 4 cut(s) 1342, 1616, 2255, 2439
SduI GDGCHC 5 cut(s) 84, 99, 1341, 1382, 2716
SfaNI GCATC 6 cut(s) 487, 631, 991, 1412, 2001, 2783
SfcI CTRYAG 2 cut(s) 1925, 2405
SfoI GGCGCC 1 cut(s) 217
SfuI TTCGAA 1 cut(s) 694
SinI GGWCC 3 cut(s) 1930, 2435, 2613
SmiMI CAYNNNNRTG 6 cut(s) 207, 210, 483, 919, 1605, 2265
SmlI CTYRAG 6 cut(s) 1469, 2017, 2291, 2552, 2569, 2618
SmoI CTYRAG 6 cut(s) 1469, 2017, 2291, 2552, 2569, 2618
SpeI ACTAGT 1 cut(s) 1634
SseBI AGGCCT 2 cut(s) 666, 2211
SsiI CCGC 2 cut(s) 116, 138
SspDI GGCGCC 1 cut(s) 215
SspI AATATT 1 cut(s) 1168
SspMI CTAG 5 cut(s) 221, 363, 1635, 1781, 1958
SstI GAGCTC 1 cut(s) 99
StuI AGGCCT 2 cut(s) 666, 2211
StyD4I CCNGG 4 cut(s) 1340, 1614, 2253, 2437
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 6 cut(s) 276, 772, 1704, 1789, 2428, 2809
TaiI ACGT 4 cut(s) 1161, 1441, 1578, 2480
TaqI TCGA 5 cut(s) 694, 2218, 2263, 2541, 2817
TaqII GACCGA 1 cut(s) 720
TatI WGTACW 4 cut(s) 515, 1704, 1905, 2060
TfiI GAWTC 7 cut(s) 419, 493, 757, 947, 953, 1290, 2752
TscAI CASTG 5 cut(s) 338, 2433, 2658, 2799, 2812
TseFI GTSAC 6 cut(s) 111, 365, 1515, 1741, 1852, 2841
TseI GCWGC 3 cut(s) 488, 2648, 2659
Tsp45I GTSAC 6 cut(s) 111, 365, 1515, 1741, 1852, 2841
TspGWI ACGGA 2 cut(s) 651, 1472
TspRI CASTG 5 cut(s) 338, 2433, 2658, 2799, 2812
Vha464I CTTAAG 1 cut(s) 1469
VpaK11BI GGWCC 3 cut(s) 1930, 2435, 2613
VspI ATTAAT 1 cut(s) 1919
XagI CCTNNNNNAGG 2 cut(s) 671, 2205
XceI RCATGY 3 cut(s) 1021, 1333, 2090
XcmI CCANNNNNNNNNTGG 4 cut(s) 155, 161, 209, 1727
XmiI GTMKAC 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 1965
XspI CTAG 5 cut(s) 221, 363, 1635, 1781, 1958
ZrmI AGTACT 1 cut(s) 2062
Zsp2I ATGCAT 2 cut(s) 624, 2776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.