RchiOBHm_Chr3g0481841

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001584
Physical Location & Seq
Reverse (-)
27923817 .. 27945797
21981 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44675

Sequence Viewer

Length: 3003 bp
ATGGCTGTGGGGAAAGAAGAGATATTGAAAGCCCGTACGGACAACAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTCAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGGCAACTACTCTTTCATCTCAGACTTGTTTCTCTTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATAGCTACTCGAAGTACATCACCGAGCATGGAGGAAGAACAAATGCTTTCACAGCTACAGAACACACTAACTATTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGGATACTTTGGAGGTTCAACCAAAAGCAAAAGGATTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAATATCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAATATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACTGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAATGGCCATGTAGGTATCTTTGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGATGTGGTTCAAACTTCGAAGTGGAGGAGGTGCACATATAGAAGGAAAATATTTAGTTGCAAGTGGAGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTTCTTTCTTTAAAGTAGTTATTGATCTCACCGATGCAGGCCAGGAGCACATGCAAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGTGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAGTTATGTGGTCAATATTTCATCAAATATGCAGATATATCCCCCAAAAGACTGGCTGGTAAGATCGTCATTGCCTTCTAACTTCAGTCCTGCCATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCTCCAAATGAGAATTTACACCTACCAGCACGTAATATGTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAATGAGAAGGTCAAAATTCCAGTTCTGTTAAGAAAGTCACCATATGCAACACTATGGTATAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTTGCTCCCCGGAAGCAGAAGTCCTGACTAGTATTTTTACTGATTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTGAGGTTGCTGGACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTTTGGTTGGTTATAATCACAAATTGAGGATTTTACTAGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGCAGTACCAAAATTACAAATTTCAACAGCCTTATGGGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGTGTGTTACACAGCAGGGAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCATGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCGAAGTGATAAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTACACTGAATGTGAAACTTGAGCTGTTTGGTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACATAACTGCTCTTAAACCGAGGAATGATTATGGTATCTGTGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGTGGGAAATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGCTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1000

Amino Acids

115.66

Weight (kDa)

6.32

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 47 - 174 7e-35 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 202 - 316 1.5e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 409 - 690 8.7e-105 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 695 - 881 6.1e-14 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 805 - 901 2.9e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1866, 2799
AccB1I GGYRCC 1 cut(s) 2821
AclI AACGTT 1 cut(s) 2585
AclWI GGATC 4 cut(s) 68, 95, 934, 2182
AcoI YGGCCR 3 cut(s) 881, 910, 2048
AcuI CTGAAG 3 cut(s) 491, 1321, 2193
AfiI CCNNNNNNNGG 6 cut(s) 265, 489, 709, 874, 1448, 2315
AflII CTTAAG 1 cut(s) 1571
AflIII ACRYGT 1 cut(s) 1431
AhlI ACTAGT 1 cut(s) 1742
AjnI CCWGG 5 cut(s) 201, 1110, 1442, 2361, 2545
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
Alw21I GWGCWC 2 cut(s) 989, 1119
Alw26I GTCTC 3 cut(s) 829, 1211, 1572
Alw44I GTGCAC 1 cut(s) 985
AlwI GGATC 4 cut(s) 68, 95, 934, 2182
ApaLI GTGCAC 1 cut(s) 985
ApeKI GCWGC 4 cut(s) 155, 524, 2756, 2767
AseI ATTAAT 2 cut(s) 354, 2027
Asp700I GAANNNNTTC 1 cut(s) 2073
AspS9I GGNCC 5 cut(s) 208, 2038, 2130, 2543, 2721
AsuC2I CCSGG 1 cut(s) 1724
AsuHPI GGTGA 8 cut(s) 280, 776, 857, 1058, 1090, 1433, 1617, 1861
AsuII TTCGAA 2 cut(s) 730, 971
AvaII GGWCC 3 cut(s) 2038, 2543, 2721
BaeGI GKGCMC 2 cut(s) 989, 2824
BalI TGGCCA 2 cut(s) 883, 2050
BanI GGYRCC 1 cut(s) 2821
BanII GRGCYC 2 cut(s) 1443, 1484
Bbv12I GWGCWC 2 cut(s) 989, 1119
BbvCI CCTCAGC 1 cut(s) 1797
BbvI GCAGC 4 cut(s) 142, 536, 2768, 2779
BccI CCATC 7 cut(s) 191, 356, 479, 1017, 2050, 2257, 2711
BciT130I CCWGG 5 cut(s) 203, 1112, 1444, 2363, 2547
BciVI GTATCC 2 cut(s) 559, 595
BclI TGATCA 1 cut(s) 1947
BcnI CCSGG 1 cut(s) 1724
BcoDI GTCTC 3 cut(s) 829, 1211, 1572
BcuI ACTAGT 1 cut(s) 1742
BfaI CTAG 4 cut(s) 246, 1743, 1889, 2066
BfmI CTRYAG 3 cut(s) 324, 2033, 2513
BfrI CTTAAG 1 cut(s) 1571
BfuI GTATCC 2 cut(s) 559, 595
BglI GCCNNNNNGGC 2 cut(s) 207, 2002
BglII AGATCT 1 cut(s) 2095
BisI GCNGC 4 cut(s) 156, 525, 2757, 2768
BlsI GCNGC 4 cut(s) 157, 526, 2758, 2769
BmcAI AGTACT 1 cut(s) 2170
Bme1390I CCNGG 6 cut(s) 203, 1112, 1444, 1724, 2363, 2547
Bme18I GGWCC 3 cut(s) 2038, 2543, 2721
BmgT120I GGNCC 5 cut(s) 208, 2038, 2130, 2543, 2721
BmiI GGNNCC 6 cut(s) 1483, 2159, 2544, 2722, 2723, 2823
BmrFI CCNGG 6 cut(s) 203, 1112, 1444, 1724, 2363, 2547
BmrI ACTGGG 1 cut(s) 2894
BmsI GCATC 7 cut(s) 415, 523, 667, 1093, 1514, 2109, 2891
BmuI ACTGGG 1 cut(s) 2894
BpmI CTGGAG 5 cut(s) 224, 511, 809, 840, 1468
Bpu10I CCTNAGC 1 cut(s) 1797
Bpu14I TTCGAA 2 cut(s) 730, 971
BpuEI CTTGAG 6 cut(s) 245, 2110, 2420, 2681, 2698, 2711
BpuMI CCSGG 1 cut(s) 1724
Bsa29I ATCGAT 2 cut(s) 2649, 2925
BsaAI YACGTR 1 cut(s) 1541
BsaJI CCNNGG 5 cut(s) 162, 1442, 1557, 1722, 2486
Bsc4I CCNNNNNNNGG 6 cut(s) 265, 489, 709, 874, 1448, 2315
Bse1I ACTGG 4 cut(s) 766, 1310, 1607, 2900
Bse3DI GCAATG 2 cut(s) 1322, 2638
BseBI CCWGG 5 cut(s) 203, 1112, 1444, 2363, 2547
BseCI ATCGAT 2 cut(s) 2649, 2925
BseDI CCNNGG 5 cut(s) 162, 1442, 1557, 1722, 2486
BseGI GGATG 5 cut(s) 172, 532, 961, 1551, 2249
BseLI CCNNNNNNNGG 6 cut(s) 265, 489, 709, 874, 1448, 2315
BseMI GCAATG 2 cut(s) 1322, 2638
BseMII CTCAG 6 cut(s) 149, 407, 1788, 2169, 2753, 2865
BseNI ACTGG 4 cut(s) 766, 1310, 1607, 2900
BseRI GAGGAG 2 cut(s) 80, 994
BseSI GKGCMC 2 cut(s) 989, 2824
BseXI GCAGC 4 cut(s) 142, 536, 2768, 2779
BsgI GTGCAG 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 2821
BshVI ATCGAT 2 cut(s) 2649, 2925
BsiHKAI GWGCWC 2 cut(s) 989, 1119
BsiSI CCGG 1 cut(s) 1724
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 794, 1689, 2734
BslI CCNNNNNNNGG 6 cut(s) 265, 489, 709, 874, 1448, 2315
BsmAI GTCTC 3 cut(s) 829, 1211, 1572
BsmFI GGGAC 3 cut(s) 794, 1689, 2734
Bsp119I TTCGAA 2 cut(s) 730, 971
Bsp1286I GDGCHC 5 cut(s) 989, 1119, 1443, 1484, 2824
Bsp19I CCATGG 1 cut(s) 162
BspCNI CTCAG 6 cut(s) 148, 406, 1789, 2170, 2754, 2866
BspDI ATCGAT 2 cut(s) 2649, 2925
BspLI GGNNCC 6 cut(s) 1483, 2159, 2544, 2722, 2723, 2823
BspMAI CTGCAG 1 cut(s) 2517
BspPI GGATC 4 cut(s) 68, 95, 934, 2182
BspT104I TTCGAA 2 cut(s) 730, 971
BspT107I GGYRCC 1 cut(s) 2821
BspTI CTTAAG 1 cut(s) 1571
BsrDI GCAATG 2 cut(s) 1322, 2638
BsrI ACTGG 4 cut(s) 766, 1310, 1607, 2900
BssECI CCNNGG 5 cut(s) 162, 1442, 1557, 1722, 2486
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 5 cut(s) 203, 1112, 1444, 2363, 2547
Bst4CI ACNGT 5 cut(s) 808, 1812, 1897, 2536, 2917
Bst6I CTCTTC 2 cut(s) 12, 366
BstAFI CTTAAG 1 cut(s) 1571
BstAPI GCANNNNNTGC 1 cut(s) 2753
BstBAI YACGTR 1 cut(s) 1541
BstBI TTCGAA 2 cut(s) 730, 971
BstC8I GCNNGC 4 cut(s) 1108, 1139, 1915, 2007
BstDSI CCRYGG 2 cut(s) 162, 1557
BstENI CCTNNNNNAGG 2 cut(s) 707, 2313
BstF5I GGATG 5 cut(s) 172, 532, 961, 1551, 2249
BstMAI GTCTC 3 cut(s) 829, 1211, 1572
BstMWI GCNNNNNNNGC 9 cut(s) 207, 320, 511, 1369, 1793, 1802, 2002, 2665, 2753
BstNI CCWGG 5 cut(s) 203, 1112, 1444, 2363, 2547
BstNSI RCATGY 3 cut(s) 1123, 1435, 2198
BstSCI CCNGG 6 cut(s) 201, 1110, 1442, 1722, 2361, 2545
BstSFI CTRYAG 3 cut(s) 324, 2033, 2513
BstSLI GKGCMC 2 cut(s) 989, 2824
BstV1I GCAGC 4 cut(s) 142, 536, 2768, 2779
BstX2I RGATCY 2 cut(s) 926, 2095
BstXI CCANNNNNNTGG 1 cut(s) 1305
BstYI RGATCY 2 cut(s) 926, 2095
Bsu15I ATCGAT 2 cut(s) 2649, 2925
BsuI GTATCC 2 cut(s) 559, 595
BsuTUI ATCGAT 2 cut(s) 2649, 2925
BtgI CCRYGG 2 cut(s) 162, 1557
BtsCI GGATG 5 cut(s) 172, 532, 961, 1551, 2249
BtsIMutI CAGTG 6 cut(s) 178, 2384, 2541, 2759, 2907, 2913
Cac8I GCNNGC 4 cut(s) 1108, 1139, 1915, 2007
Cfr13I GGNCC 5 cut(s) 208, 2038, 2130, 2543, 2721
ClaI ATCGAT 2 cut(s) 2649, 2925
DraI TTTAAA 2 cut(s) 1081, 1921
EaeI YGGCCR 3 cut(s) 881, 910, 2048
Eam1104I CTCTTC 2 cut(s) 12, 366
EarI CTCTTC 2 cut(s) 12, 366
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 2 cut(s) 702, 2319
Eco24I GRGCYC 2 cut(s) 1443, 1484
Eco47I GGWCC 3 cut(s) 2038, 2543, 2721
Eco57I CTGAAG 3 cut(s) 491, 1321, 2193
EcoNI CCTNNNNNAGG 2 cut(s) 707, 2313
EcoO109I RGGNCCY 3 cut(s) 208, 2130, 2721
EcoRI GAATTC 3 cut(s) 1488, 2338, 2621
EcoRII CCWGG 5 cut(s) 201, 1110, 1442, 2361, 2545
EcoT14I CCWWGG 1 cut(s) 162
EcoT22I ATGCAT 2 cut(s) 660, 2884
EcoT38I GRGCYC 2 cut(s) 1443, 1484
ErhI CCWWGG 1 cut(s) 162
FalI AAGNNNNNCTT 4 cut(s) 669, 701, 2966, 2998
FaqI GGGAC 3 cut(s) 794, 1689, 2734
FauNDI CATATG 2 cut(s) 231, 1630
FbaI TGATCA 1 cut(s) 1947
Fnu4HI GCNGC 4 cut(s) 156, 525, 2757, 2768
FokI GGATG 5 cut(s) 179, 519, 968, 1538, 2236
FriOI GRGCYC 2 cut(s) 1443, 1484
Fsp4HI GCNGC 4 cut(s) 156, 525, 2757, 2768
FspBI CTAG 4 cut(s) 246, 1743, 1889, 2066
GluI GCNGC 4 cut(s) 156, 525, 2757, 2768
GsuI CTGGAG 5 cut(s) 224, 511, 809, 840, 1468
HapII CCGG 1 cut(s) 1724
HindIII AAGCTT 5 cut(s) 1370, 1915, 2279, 2597, 2657
HpaII CCGG 1 cut(s) 1724
HphI GGTGA 8 cut(s) 280, 776, 857, 1058, 1090, 1433, 1617, 1861
Hpy166II GTNNAC 4 cut(s) 987, 2353, 2510, 2846
Hpy8I GTNNAC 4 cut(s) 987, 2353, 2510, 2846
HpyCH4III ACNGT 5 cut(s) 808, 1812, 1897, 2536, 2917
HpyCH4IV ACGT 3 cut(s) 1540, 1683, 2585
HpyF10VI GCNNNNNNNGC 9 cut(s) 207, 320, 511, 1369, 1793, 1802, 2002, 2665, 2753
HpySE526I ACGT 3 cut(s) 1540, 1683, 2585
KflI GGGWCCC 1 cut(s) 2721
Ksp22I TGATCA 1 cut(s) 1947
LmnI GCTCC 4 cut(s) 1114, 1487, 1724, 2900
Lsp1109I GCAGC 4 cut(s) 142, 536, 2768, 2779
LweI GCATC 7 cut(s) 415, 523, 667, 1093, 1514, 2109, 2891
MaeI CTAG 4 cut(s) 246, 1743, 1889, 2066
MaeII ACGT 3 cut(s) 1540, 1683, 2585
MaeIII GTNAC 7 cut(s) 1161, 1623, 1849, 1960, 2144, 2465, 2949
MfeI CAATTG 2 cut(s) 559, 1699
MflI RGATCY 2 cut(s) 926, 2095
MhlI GDGCHC 5 cut(s) 989, 1119, 1443, 1484, 2824
MlsI TGGCCA 2 cut(s) 883, 2050
MluNI TGGCCA 2 cut(s) 883, 2050
MlyI GAGTC 3 cut(s) 1412, 2189, 2280
Mox20I TGGCCA 2 cut(s) 883, 2050
Mph1103I ATGCAT 2 cut(s) 660, 2884
MroXI GAANNNNTTC 1 cut(s) 2073
MscI TGGCCA 2 cut(s) 883, 2050
MslI CAYNNNNRTG 2 cut(s) 519, 2373
Msp20I TGGCCA 2 cut(s) 883, 2050
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 1571
MspI CCGG 1 cut(s) 1724
MspR9I CCNGG 6 cut(s) 203, 1112, 1444, 1724, 2363, 2547
MunI CAATTG 2 cut(s) 559, 1699
MvaI CCWGG 5 cut(s) 203, 1112, 1444, 2363, 2547
MwoI GCNNNNNNNGC 9 cut(s) 207, 320, 511, 1369, 1793, 1802, 2002, 2665, 2753
NciI CCSGG 1 cut(s) 1724
NcoI CCATGG 1 cut(s) 162
NdeI CATATG 2 cut(s) 231, 1630
NlaIV GGNNCC 6 cut(s) 1483, 2159, 2544, 2722, 2723, 2823
NmuCI GTSAC 4 cut(s) 1623, 1849, 1960, 2949
NsiI ATGCAT 2 cut(s) 660, 2884
NspI RCATGY 3 cut(s) 1123, 1435, 2198
NspV TTCGAA 2 cut(s) 730, 971
PceI AGGCCT 2 cut(s) 702, 2319
PciI ACATGT 1 cut(s) 1431
PdmI GAANNNNTTC 1 cut(s) 2073
PfeI GAWTC 7 cut(s) 455, 529, 793, 1049, 1055, 1392, 2860
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2545
PkrI GCNGC 4 cut(s) 157, 526, 2758, 2769
PleI GAGTC 3 cut(s) 1411, 2188, 2279
PpsI GAGTC 3 cut(s) 1411, 2188, 2279
Ppu21I YACGTR 1 cut(s) 1541
PpuMI RGGWCCY 1 cut(s) 2721
PscI ACATGT 1 cut(s) 1431
PshBI ATTAAT 2 cut(s) 354, 2027
PsiI TTATAA 2 cut(s) 1866, 2799
Psp1406I AACGTT 1 cut(s) 2585
Psp5II RGGWCCY 1 cut(s) 2721
Psp6I CCWGG 5 cut(s) 201, 1110, 1442, 2361, 2545
PspGI CCWGG 5 cut(s) 201, 1110, 1442, 2361, 2545
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1483, 2159, 2544, 2722, 2723, 2823
PspPI GGNCC 5 cut(s) 208, 2038, 2130, 2543, 2721
PspPPI RGGWCCY 1 cut(s) 2721
PstI CTGCAG 1 cut(s) 2517
PsuI RGATCY 2 cut(s) 926, 2095
PvuII CAGCTG 1 cut(s) 422
RseI CAYNNNNRTG 2 cut(s) 519, 2373
SatI GCNGC 4 cut(s) 156, 525, 2757, 2768
Sau96I GGNCC 5 cut(s) 208, 2038, 2130, 2543, 2721
ScaI AGTACT 1 cut(s) 2170
SchI GAGTC 3 cut(s) 1412, 2189, 2280
ScrFI CCNGG 6 cut(s) 203, 1112, 1444, 1724, 2363, 2547
SduI GDGCHC 5 cut(s) 989, 1119, 1443, 1484, 2824
SfaNI GCATC 7 cut(s) 415, 523, 667, 1093, 1514, 2109, 2891
SfcI CTRYAG 3 cut(s) 324, 2033, 2513
SfiI GGCCNNNNNGGCC 1 cut(s) 207
SfuI TTCGAA 2 cut(s) 730, 971
SinI GGWCC 3 cut(s) 2038, 2543, 2721
SmiMI CAYNNNNRTG 2 cut(s) 519, 2373
SmlI CTYRAG 7 cut(s) 224, 1571, 2125, 2399, 2660, 2677, 2726
SmoI CTYRAG 7 cut(s) 224, 1571, 2125, 2399, 2660, 2677, 2726
SpeI ACTAGT 1 cut(s) 1742
SseBI AGGCCT 2 cut(s) 702, 2319
SspI AATATT 3 cut(s) 736, 1005, 1270
SspMI CTAG 4 cut(s) 246, 1743, 1889, 2066
StuI AGGCCT 2 cut(s) 702, 2319
StyD4I CCNGG 6 cut(s) 201, 1110, 1442, 1722, 2361, 2545
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 5 cut(s) 808, 1812, 1897, 2536, 2917
TaiI ACGT 3 cut(s) 1543, 1686, 2588
TaqI TCGA 7 cut(s) 278, 730, 971, 2326, 2371, 2649, 2925
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 6 cut(s) 51, 282, 551, 1812, 2013, 2168
TfiI GAWTC 7 cut(s) 455, 529, 793, 1049, 1055, 1392, 2860
TscAI CASTG 6 cut(s) 178, 2391, 2541, 2766, 2907, 2920
TseFI GTSAC 4 cut(s) 1623, 1849, 1960, 2949
TseI GCWGC 4 cut(s) 155, 524, 2756, 2767
Tsp45I GTSAC 4 cut(s) 1623, 1849, 1960, 2949
TspGWI ACGGA 3 cut(s) 53, 687, 1574
TspRI CASTG 6 cut(s) 178, 2391, 2541, 2766, 2907, 2920
Vha464I CTTAAG 1 cut(s) 1571
VneI GTGCAC 1 cut(s) 985
VpaK11BI GGWCC 3 cut(s) 2038, 2543, 2721
VspI ATTAAT 2 cut(s) 354, 2027
XagI CCTNNNNNAGG 2 cut(s) 707, 2313
XceI RCATGY 3 cut(s) 1123, 1435, 2198
XcmI CCANNNNNNNNNTGG 2 cut(s) 1354, 1835
XmnI GAANNNNTTC 1 cut(s) 2073
XspI CTAG 4 cut(s) 246, 1743, 1889, 2066
ZrmI AGTACT 1 cut(s) 2170
Zsp2I ATGCAT 2 cut(s) 660, 2884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.