MD14G1071400.v1.1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001584
Physical Location & Seq
Forward (+)
7872795 .. 7885104
12310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1071400.v1.1.491

Sequence Viewer

Length: 2928 bp
ATGGCGGTCGGCAAGGAAGTGGTGGAGGAAATAGTGAAAGCCCGTACGGACAAGAGGGAGTACAGGAGGATCGTCCTCCCTAACTCTCTCGAAGCGCTTTTGATCAGCGATCCCGATACTGACAAGGCAAAGCTTTGTGCTGCTTCCATGGATGTCAACGTTGGTGCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCGAGTGAAAAGTACCCTTTGGAGGATAGTTACTCCAAGTACATCACTGAGCATGGAGGACGAACAAATGCTTATACAGCTTCTGAGCACACCAACTATTATTTTGACGTCAATTCTGATGGTTTTGAAGAGGCTTTGGACAGGTTGATGCTTTTGTTTCTATTTGCTCAGTTCTTTATTAACCCATTGATGTCAGCTGATGCTACAATGAGGGAAATCAAAGCTGTTGATTCTGAGAATCAGAAAAACCTGTTGTCTGATGGATGGAGAATGAACCAGCTCCAGAAACATCTAAGTGCGGCTGACCATCCGTACCATAAATTTAGTACAGGGAATTGGGAAACTTTGGAGGTCCGTCCAAAAGCAAAAGGATTGGATACCAGAGCTGAGCTTAATAAGTTCTATGAACAGTATTATTCCGCTAACGTCATGCATCTTGTCATTTATGGAAAAGAAAGCCTAGATAAAATTCAAGCCACTGTAGAGGACAAGTTTAAGGGTATTCGAAACATTGACCGCAGCTGCCTTCATTTCAGTGGTGAGCCTTGCACATCAGAACATTTACAGATTCTTGTCAAAACTGTCCCAATCAAAGAGGGACATAAGCTGGGACTTGTGTGGCCAATAGCTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTAGTCATCTCATTGGCCACGAAGCAGAGGGGTCTTTGTATGCCGTCTTGAAAACTTTGGGATGGGCAACAGGTTTGTGTGCTGGAGAATCAGATTCGACTTTGGATTTTTCTTTCTTTAGAGTAGATATGGATCTCACTGACGCGGGTCATGAGCATATGCAAGACATAGTAGGCTTGCTTTTCAAATACATTTCCCTCTTACACCAGTCGGGTATCTGCAAATGGATTTTTGATGAGCTTTCTGCAGTTTGCGAGACAAAGTTTCATTATCAGGACAAAAGTCAGCCTATTAATTATGTCGTCAGCATTTCGCCAAATATGCAGAAATATCCCCCAAGAGATTGGCTAGTAAAATCAACCTTGCCTTCTAACTTCAGTCCAGACACTATCCAAATGGTGCTTAACAAGCTTTCTCCAGAAAATGTCAGAATTTTCTGGGTATCAAAAAAATTTGAAGGTCATACTAACATGACTGAGCCATGGTATGGGACCGCTTATTCCGTCGGGAAAATTAGTGGCTCCATGATTCAGGAATGGATAGTATCTTCCCCAAATGAGAATCTGCACCTACCAGCCCATAATGTGTTCATCCCCACGGACTTGTCACTTAAGAATGATCATGAGAAGGCCAACTGTCCTGTTCTGTTAAGAAAGTCGCCGTGTTCAACACTCTGGTACAAGCCGGATACAATGTTCTTTACTCCTAAGGCTTACGTTAGGATTCATTTCACTTGTCCCCACACAAGTGAATCCCCTGAAACAGAAGTCTTAACTAAAATGTTTACTCGGTTGTTGATGGATTACTTGAATGAATATGCTTACTATGCTGAGGTTGCTGAACTGTTTTACGCAATAACCCAAACAGATAGCGGGTTTCAGGTGACTTTGGTTGGCTATAACCACAAATTAAGAATTTTACTAGAAACTGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGATAGGTTCGCTGTCATCAAGGAAATGCTCATGAAGGAGTACCAAAACTTGAAATTCAAACAGCCTTATGAGCAGGCTATGAATTACACCGCATTAATTCTACATGATCATACTTCTCCATGGATGGAAGAACTTGAAGCTCTTCCTCACCTTCAAGTTGAAGATCTTGCTAAGTTTGTTCCTCTGATGCTCTCGAGGGCCTTCTTAGAGTGTTATACAGCAGGAAACCTTGAAAGGAATGAAGCTGAGTCAATGATCCAACATATTGAAGATGTTTTCTTTAAGGGCTCAAACCTGATTTCCCAACCTTTGTTCCCATCCCAGCATTTGACGAATAGAGTCATGAAGCTTGAAAAGGGCACGAACTACTTCTACCCTGTGGAAGGCCTTAATCCGAGTGATGAGAATTCTGCCCTTGTCCACTATATCCAGGTTCATCGGGATGATTTTATGCTGAATGTGAAACTTCAGCTGTTTGCTCTTATTGCAAAGCAGCCTGCCTTCCACCAGCTTAGATCCGTTGAGCAACTTGGTTACATCACGGCCCTCGTGCAGAGGAATGATTTTGGTATCCGTGGGGTGCAGTTTATTATACAATCTACAGTGAAGGATCCAGCACATATTGATTTGAGAGTGGAGGAATTCCTCAAGGGGTTCGAGACTAAACTTTATGAGATGCCAAGTGACGAATTCAAGAGCAATGTAAATGCTTTGATCGACATGAAGCTTGAGAAGCACAAGAACTTAAGGGAAGAAGCTGGATTTTATTGGACGGAGATTTCTGATGGGACCCTCAAGTTTGATAGGAAAGAGTCTGAGATTGCAGCACTGAGGCAGCTTACGCAGCAAGAATTGATAGATTTTTTCAATGAGCATATAAAAGTTGGGGCACCTCAAAAGAGGTCATTAAGTGTACGAGTGTACGGGAACTCACATTCATCCGAGCACACTACGGATGGAAGTTCACCTGTGAAACCTTGCTATGTCAAAATCGACGATATATTCAGCTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGTAAATCATGTGAAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

976

Amino Acids

112.07

Weight (kDa)

5.94

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 41 - 176 1.2e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 203 - 380 6.3e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 388 - 668 1.5e-102 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 672 - 859 1.6e-09 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 779 - 878 1.1e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 2752
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccII CGCG 1 cut(s) 1043
AciI CCGC 8 cut(s) 5, 527, 648, 745, 1043, 1392, 1770, 1953
AclI AACGTT 1 cut(s) 159
AclWI GGATC 7 cut(s) 77, 104, 1038, 2113, 2373, 2468, 2481
AcoI YGGCCR 2 cut(s) 848, 913
AcuI CTGAAG 3 cut(s) 1258, 2315, 2857
AcyI GRCGYC 1 cut(s) 336
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 5 cut(s) 250, 877, 1385, 2246, 2894
AflII CTTAAG 2 cut(s) 1508, 2608
AjnI CCWGG 2 cut(s) 186, 2292
AleI CACNNNNGTG 1 cut(s) 1644
AloI GAACNNNNNNTCC 2 cut(s) 1577, 1609
Alw21I GWGCWC 2 cut(s) 318, 2811
Alw26I GTCTC 2 cut(s) 1148, 2516
AlwI GGATC 7 cut(s) 77, 104, 1038, 2113, 2373, 2468, 2481
AlwNI CAGNNNCTG 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 2056
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 9 cut(s) 184, 193, 848, 884, 913, 1527, 2061, 2248, 2406
ApeKI GCWGC 7 cut(s) 140, 747, 750, 2356, 2687, 2698, 2707
AseI ATTAAT 2 cut(s) 1191, 1958
Asp700I GAANNNNTTC 2 cut(s) 2004, 2231
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 7 cut(s) 193, 580, 884, 1389, 2061, 2407, 2652
AsuHPI GGTGA 5 cut(s) 779, 860, 1792, 2003, 2820
AsuII TTCGAA 1 cut(s) 733
AvaI CYCGRG 1 cut(s) 2056
AvaII GGWCC 3 cut(s) 580, 1389, 2652
AxyI CCTNAGG 1 cut(s) 1605
BaeGI GKGCMC 2 cut(s) 2225, 2755
BalI TGGCCA 2 cut(s) 850, 915
BamHI GGATCC 1 cut(s) 2473
BanI GGYRCC 1 cut(s) 2752
BanII GRGCYC 1 cut(s) 2153
BauI CACGAG 1 cut(s) 2411
Bbv12I GWGCWC 2 cut(s) 318, 2811
BbvCI CCTCAGC 1 cut(s) 1728
BbvI GCAGC 7 cut(s) 127, 737, 759, 2368, 2699, 2710, 2719
BceAI ACGGC 3 cut(s) 926, 1543, 2421
BciT130I CCWGG 2 cut(s) 188, 2294
BciVI GTATCC 3 cut(s) 598, 1579, 2444
BclI TGATCA 3 cut(s) 102, 1516, 1969
BcoDI GTCTC 2 cut(s) 1148, 2516
BfaI CTAG 3 cut(s) 689, 1247, 1820
BfmI CTRYAG 4 cut(s) 708, 1143, 1827, 2463
BfoI RGCGCY 1 cut(s) 98
BfrI CTTAAG 2 cut(s) 1508, 2608
BfuI GTATCC 3 cut(s) 598, 1579, 2444
BglI GCCNNNNNGGC 1 cut(s) 192
BglII AGATCT 1 cut(s) 2026
BisI GCNGC 8 cut(s) 141, 528, 748, 751, 2357, 2688, 2699, 2708
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 8 cut(s) 142, 529, 749, 752, 2358, 2689, 2700, 2709
Bme1390I CCNGG 2 cut(s) 188, 2294
Bme18I GGWCC 3 cut(s) 580, 1389, 2652
BmeT110I CYCGRG 1 cut(s) 2056
BmgT120I GGNCC 7 cut(s) 193, 580, 884, 1389, 2061, 2407, 2652
BmiI GGNNCC 6 cut(s) 1390, 1420, 2475, 2653, 2654, 2754
BmrFI CCNGG 2 cut(s) 188, 2294
BmsI GCATC 5 cut(s) 366, 418, 670, 2040, 2529
BoxI GACNNNNGTC 2 cut(s) 1044, 1179
BpmI CTGGAG 5 cut(s) 209, 494, 843, 1002, 1299
Bpu10I CCTNAGC 1 cut(s) 1728
Bpu1102I GCTNAGC 1 cut(s) 615
Bpu14I TTCGAA 1 cut(s) 733
BpuEI CTTGAG 4 cut(s) 230, 2495, 2612, 2642
BsaBI GATNNNNATC 1 cut(s) 1029
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 5 cut(s) 147, 1379, 1494, 1982, 2437
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 877, 1385, 2246, 2894
Bse1I ACTGG 1 cut(s) 1105
Bse21I CCTNAGG 1 cut(s) 1605
Bse3DI GCAATG 1 cut(s) 2569
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 2 cut(s) 188, 2294
BseDI CCNNGG 5 cut(s) 147, 1379, 1494, 1982, 2437
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 5 cut(s) 250, 877, 1385, 2246, 2894
BseMI GCAATG 1 cut(s) 2569
BseNI ACTGG 1 cut(s) 1105
BseSI GKGCMC 2 cut(s) 2225, 2755
BseXI GCAGC 7 cut(s) 127, 737, 759, 2368, 2699, 2710, 2719
BseYI CCCAGC 2 cut(s) 835, 2184
BsgI GTGCAG 3 cut(s) 1448, 2435, 2465
Bsh1236I CGCG 1 cut(s) 1043
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 9 cut(s) 186, 195, 850, 886, 915, 1529, 2063, 2250, 2408
BshNI GGYRCC 1 cut(s) 2752
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 318, 2811
BsiHKCI CYCGRG 1 cut(s) 2056
BsiSI CCGG 1 cut(s) 1583
BsiWI CGTACG 1 cut(s) 44
BslFI GGGAC 6 cut(s) 797, 840, 852, 1402, 1620, 2665
BslI CCNNNNNNNGG 5 cut(s) 250, 877, 1385, 2246, 2894
BsmAI GTCTC 2 cut(s) 1148, 2516
BsmFI GGGAC 6 cut(s) 797, 840, 852, 1402, 1620, 2665
BsnI GGCC 9 cut(s) 186, 195, 850, 886, 915, 1529, 2063, 2250, 2408
BsoBI CYCGRG 1 cut(s) 2056
Bsp119I TTCGAA 1 cut(s) 733
Bsp1286I GDGCHC 5 cut(s) 318, 2153, 2225, 2755, 2811
Bsp1720I GCTNAGC 1 cut(s) 615
Bsp19I CCATGG 3 cut(s) 147, 1379, 1982
BspACI CCGC 8 cut(s) 5, 527, 648, 745, 1043, 1392, 1770, 1953
BspANI GGCC 9 cut(s) 186, 195, 850, 886, 915, 1529, 2063, 2250, 2408
BspFNI CGCG 1 cut(s) 1043
BspHI TCATGA 4 cut(s) 1048, 1519, 1893, 2205
BspLI GGNNCC 6 cut(s) 1390, 1420, 2475, 2653, 2654, 2754
BspMAI CTGCAG 1 cut(s) 1147
BspPI GGATC 7 cut(s) 77, 104, 1038, 2113, 2373, 2468, 2481
BspQI GCTCTTC 1 cut(s) 2010
BspT104I TTCGAA 1 cut(s) 733
BspT107I GGYRCC 1 cut(s) 2752
BspTI CTTAAG 2 cut(s) 1508, 2608
BsrDI GCAATG 1 cut(s) 2569
BsrI ACTGG 1 cut(s) 1105
BssECI CCNNGG 5 cut(s) 147, 1379, 1494, 1982, 2437
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 2411
BssT1I CCWWGG 3 cut(s) 147, 1379, 1982
Bst2BI CACGAG 1 cut(s) 2411
Bst2UI CCWGG 2 cut(s) 188, 2294
Bst4CI ACNGT 7 cut(s) 639, 709, 811, 1535, 1743, 1828, 2467
Bst6I CTCTTC 2 cut(s) 351, 2010
BstACI GRCGYC 1 cut(s) 336
BstAFI CTTAAG 2 cut(s) 1508, 2608
BstBI TTCGAA 1 cut(s) 733
BstC8I GCNNGC 4 cut(s) 1076, 1846, 1938, 2361
BstDSI CCRYGG 5 cut(s) 147, 1379, 1494, 1982, 2437
BstENI CCTNNNNNAGG 1 cut(s) 2244
BstFNI CGCG 1 cut(s) 1043
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstMAI GTCTC 2 cut(s) 1148, 2516
BstMCI CGRYCG 1 cut(s) 9
BstNI CCWGG 2 cut(s) 188, 2294
BstPAI GACNNNNGTC 2 cut(s) 1044, 1179
BstSCI CCNGG 2 cut(s) 186, 2292
BstSFI CTRYAG 4 cut(s) 708, 1143, 1827, 2463
BstSLI GKGCMC 2 cut(s) 2225, 2755
BstUI CGCG 1 cut(s) 1043
BstV1I GCAGC 7 cut(s) 127, 737, 759, 2368, 2699, 2710, 2719
BstX2I RGATCY 4 cut(s) 1030, 2026, 2378, 2473
BstXI CCANNNNNNTGG 1 cut(s) 1242
BstYI RGATCY 4 cut(s) 1030, 2026, 2378, 2473
Bsu36I CCTNAGG 1 cut(s) 1605
BsuI GTATCC 3 cut(s) 598, 1579, 2444
BsuRI GGCC 9 cut(s) 186, 195, 850, 886, 915, 1529, 2063, 2250, 2408
BtgI CCRYGG 5 cut(s) 147, 1379, 1494, 1982, 2437
BtsIMutI CAGTG 6 cut(s) 273, 705, 769, 1035, 2472, 2690
Cac8I GCNNGC 4 cut(s) 1076, 1846, 1938, 2361
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 4 cut(s) 1048, 1519, 1893, 2205
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 7 cut(s) 193, 580, 884, 1389, 2061, 2407, 2652
CseI GACGC 1 cut(s) 1049
DraI TTTAAA 1 cut(s) 1852
EaeI YGGCCR 2 cut(s) 848, 913
Eam1104I CTCTTC 2 cut(s) 351, 2010
EarI CTCTTC 2 cut(s) 351, 2010
Eco130I CCWWGG 3 cut(s) 147, 1379, 1982
Eco147I AGGCCT 1 cut(s) 2250
Eco24I GRGCYC 1 cut(s) 2153
Eco47I GGWCC 3 cut(s) 580, 1389, 2652
Eco47III AGCGCT 1 cut(s) 96
Eco57I CTGAAG 3 cut(s) 1258, 2315, 2857
Eco81I CCTNAGG 1 cut(s) 1605
Eco88I CYCGRG 1 cut(s) 2056
EcoNI CCTNNNNNAGG 1 cut(s) 2244
EcoO109I RGGNCCY 3 cut(s) 193, 2061, 2652
EcoRI GAATTC 3 cut(s) 2269, 2504, 2552
EcoRII CCWGG 2 cut(s) 186, 2292
EcoT14I CCWWGG 3 cut(s) 147, 1379, 1982
EcoT22I ATGCAT 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 2153
ErhI CCWWGG 3 cut(s) 147, 1379, 1982
FaqI GGGAC 6 cut(s) 797, 840, 852, 1402, 1620, 2665
FauI CCCGC 2 cut(s) 1036, 1763
FauNDI CATATG 2 cut(s) 216, 1056
FbaI TGATCA 3 cut(s) 102, 1516, 1969
Fnu4HI GCNGC 8 cut(s) 141, 528, 748, 751, 2357, 2688, 2699, 2708
FriOI GRGCYC 1 cut(s) 2153
Fsp4HI GCNGC 8 cut(s) 141, 528, 748, 751, 2357, 2688, 2699, 2708
FspBI CTAG 3 cut(s) 689, 1247, 1820
GlaI GCGC 1 cut(s) 96
GluI GCNGC 8 cut(s) 141, 528, 748, 751, 2357, 2688, 2699, 2708
GsaI CCCAGC 2 cut(s) 839, 2188
GsuI CTGGAG 5 cut(s) 209, 494, 843, 1002, 1299
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 9 cut(s) 186, 195, 850, 886, 915, 1529, 2063, 2250, 2408
HapII CCGG 1 cut(s) 1583
HgaI GACGC 1 cut(s) 1049
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 336
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 5 cut(s) 131, 1307, 1846, 2210, 2588
HpaII CCGG 1 cut(s) 1583
HphI GGTGA 5 cut(s) 779, 860, 1792, 2003, 2820
Hpy166II GTNNAC 6 cut(s) 157, 1683, 2284, 2777, 2785, 2828
Hpy8I GTNNAC 6 cut(s) 157, 1683, 2284, 2777, 2785, 2828
Hpy99I CGWCG 2 cut(s) 1406, 2861
HpyCH4III ACNGT 7 cut(s) 639, 709, 811, 1535, 1743, 1828, 2467
HpyCH4IV ACGT 4 cut(s) 159, 336, 654, 1614
HpySE526I ACGT 4 cut(s) 159, 336, 654, 1614
Hsp92I GRCGYC 1 cut(s) 336
HspAI GCGC 1 cut(s) 95
KflI GGGWCCC 1 cut(s) 2652
Ksp22I TGATCA 3 cut(s) 102, 1516, 1969
LguI GCTCTTC 1 cut(s) 2010
LmnI GCTCC 3 cut(s) 513, 862, 1424
Lsp1109I GCAGC 7 cut(s) 127, 737, 759, 2368, 2699, 2710, 2719
LweI GCATC 5 cut(s) 366, 418, 670, 2040, 2529
MaeI CTAG 3 cut(s) 689, 1247, 1820
MaeII ACGT 4 cut(s) 159, 336, 654, 1614
MaeIII GTNAC 6 cut(s) 257, 1503, 1780, 2396, 2546, 2880
MboII GAAGA 8 cut(s) 368, 1437, 1849, 1997, 2003, 2036, 2144, 2627
MflI RGATCY 4 cut(s) 1030, 2026, 2378, 2473
MhlI GDGCHC 5 cut(s) 318, 2153, 2225, 2755, 2811
MlsI TGGCCA 2 cut(s) 850, 915
MluNI TGGCCA 2 cut(s) 850, 915
MlyI GAGTC 3 cut(s) 2120, 2211, 2684
MmeI TCCRAC 1 cut(s) 2146
Mox20I TGGCCA 2 cut(s) 850, 915
Mph1103I ATGCAT 1 cut(s) 663
MroXI GAANNNNTTC 2 cut(s) 2004, 2231
MscI TGGCCA 2 cut(s) 850, 915
MslI CAYNNNNRTG 4 cut(s) 522, 762, 1644, 2304
Msp20I TGGCCA 2 cut(s) 850, 915
MspA1I CMGCKG 3 cut(s) 425, 750, 2335
MspCI CTTAAG 2 cut(s) 1508, 2608
MspI CCGG 1 cut(s) 1583
MspR9I CCNGG 2 cut(s) 188, 2294
MvaI CCWGG 2 cut(s) 188, 2294
MvnI CGCG 1 cut(s) 1043
NcoI CCATGG 3 cut(s) 147, 1379, 1982
NdeI CATATG 2 cut(s) 216, 1056
NlaIV GGNNCC 6 cut(s) 1390, 1420, 2475, 2653, 2654, 2754
NmuCI GTSAC 4 cut(s) 1503, 1780, 2546, 2880
NsiI ATGCAT 1 cut(s) 663
NspV TTCGAA 1 cut(s) 733
OliI CACNNNNGTG 1 cut(s) 1644
PaeR7I CTCGAG 1 cut(s) 2056
PagI TCATGA 4 cut(s) 1048, 1519, 1893, 2205
PceI AGGCCT 1 cut(s) 2250
PciSI GCTCTTC 1 cut(s) 2010
PdmI GAANNNNTTC 2 cut(s) 2004, 2231
PfeI GAWTC 9 cut(s) 458, 466, 796, 986, 992, 1426, 1459, 1621, 1649
Pfl23II CGTACG 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 1385
PkrI GCNGC 8 cut(s) 142, 529, 749, 752, 2358, 2689, 2700, 2709
PleI GAGTC 3 cut(s) 2119, 2210, 2683
PpsI GAGTC 3 cut(s) 2119, 2210, 2683
PpuMI RGGWCCY 1 cut(s) 2652
PshAI GACNNNNGTC 2 cut(s) 1044, 1179
PshBI ATTAAT 2 cut(s) 1191, 1958
Psp1406I AACGTT 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 2652
Psp6I CCWGG 2 cut(s) 186, 2292
PspFI CCCAGC 2 cut(s) 835, 2184
PspGI CCWGG 2 cut(s) 186, 2292
PspLI CGTACG 1 cut(s) 44
PspN4I GGNNCC 6 cut(s) 1390, 1420, 2475, 2653, 2654, 2754
PspPI GGNCC 7 cut(s) 193, 580, 884, 1389, 2061, 2407, 2652
PspPPI RGGWCCY 1 cut(s) 2652
PsrI GAACNNNNNNTAC 2 cut(s) 2219, 2251
PstI CTGCAG 1 cut(s) 1147
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 4 cut(s) 1030, 2026, 2378, 2473
PvuII CAGCTG 3 cut(s) 425, 750, 2335
RseI CAYNNNNRTG 4 cut(s) 522, 762, 1644, 2304
SapI GCTCTTC 1 cut(s) 2010
SatI GCNGC 8 cut(s) 141, 528, 748, 751, 2357, 2688, 2699, 2708
Sau96I GGNCC 7 cut(s) 193, 580, 884, 1389, 2061, 2407, 2652
SchI GAGTC 3 cut(s) 2120, 2211, 2684
ScrFI CCNGG 2 cut(s) 188, 2294
SduI GDGCHC 5 cut(s) 318, 2153, 2225, 2755, 2811
SfaNI GCATC 5 cut(s) 366, 418, 670, 2040, 2529
SfcI CTRYAG 4 cut(s) 708, 1143, 1827, 2463
SfiI GGCCNNNNNGGCC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 2056
SfuI TTCGAA 1 cut(s) 733
SinI GGWCC 3 cut(s) 580, 1389, 2652
SlaI CTCGAG 1 cut(s) 2056
SmiMI CAYNNNNRTG 4 cut(s) 522, 762, 1644, 2304
SmlI CTYRAG 7 cut(s) 209, 1508, 2056, 2510, 2591, 2608, 2657
SmoI CTYRAG 7 cut(s) 209, 1508, 2056, 2510, 2591, 2608, 2657
SseBI AGGCCT 1 cut(s) 2250
SsiI CCGC 8 cut(s) 5, 527, 648, 745, 1043, 1392, 1770, 1953
SspMI CTAG 3 cut(s) 689, 1247, 1820
StuI AGGCCT 1 cut(s) 2250
StyD4I CCNGG 2 cut(s) 186, 2292
StyI CCWWGG 3 cut(s) 147, 1379, 1982
TaaI ACNGT 7 cut(s) 639, 709, 811, 1535, 1743, 1828, 2467
TaiI ACGT 4 cut(s) 162, 339, 657, 1617
TaqI TCGA 7 cut(s) 90, 733, 995, 2057, 2520, 2580, 2856
TatI WGTACW 3 cut(s) 60, 267, 554
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 9 cut(s) 458, 466, 796, 986, 992, 1426, 1459, 1621, 1649
TscAI CASTG 6 cut(s) 280, 712, 769, 1042, 2472, 2697
TseFI GTSAC 4 cut(s) 1503, 1780, 2546, 2880
TseI GCWGC 7 cut(s) 140, 747, 750, 2356, 2687, 2698, 2707
Tsp45I GTSAC 4 cut(s) 1503, 1780, 2546, 2880
TspGWI ACGGA 9 cut(s) 62, 528, 572, 1390, 1511, 2371, 2426, 2651, 2831
TspRI CASTG 6 cut(s) 280, 712, 769, 1042, 2472, 2697
Van91I CCANNNNNTGG 1 cut(s) 1385
Vha464I CTTAAG 2 cut(s) 1508, 2608
VpaK11BI GGWCC 3 cut(s) 580, 1389, 2652
VspI ATTAAT 2 cut(s) 1191, 1958
XagI CCTNNNNNAGG 1 cut(s) 2244
XhoI CTCGAG 1 cut(s) 2056
XmnI GAANNNNTTC 2 cut(s) 2004, 2231
XspI CTAG 3 cut(s) 689, 1247, 1820
ZraI GACGTC 1 cut(s) 337
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.