Rroxscaffold_6G00400030

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001584
Physical Location & Seq
Reverse (-)
22126470 .. 22139570
13101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400030.1

Sequence Viewer

Length: 2769 bp
ATGGCTGTGGGGAAAGAAGAGATATTGAAAGCCCGTACGGACAGCAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTCCTCATCAGCGATCCTGATACTGACAAGGCAACTACTCTTTCATCTCAGACTTGTTTCTCTTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGTACCCTTTGGAGGATACTTACTCGAAGTACATCACTGAGCATGGAGGAAGAACAAATGCTTTCACAGCTGCAGAACACACCAACTTTTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCCGATGGCTGGAGAATGAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGAATACTTTGGAGGTTCGACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATATGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGAGCACATGCGAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGCGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCAGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATGCAAACATGTTCGAGCCCTGGTATGAAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATGAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCAACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGCCCAGTTCTGTTAAGAAAGTCACCATATACAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATATGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGAAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTCAGGTTGCTGAACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTCTTGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCAATGATGCTCTCAAGGGCCTTCTTAGAGTGTTACACAGCAGAGGGATTTTGTTTTTCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCATGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTGCTTGTTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACATAACTGCTCTTTTACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGTTTCTTGGTTGTAGTTTTGACCCCGAGGGAAAAGGTGTGGAGTTTTTGGGTATAGTTGTATGCAGAATTGACCTTATTGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAGTCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

922

Amino Acids

105.98

Weight (kDa)

5.89

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 47 - 174 5.9e-34 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 202 - 379 4e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 387 - 630 7.3e-90 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 701 - 799 7.3e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1794, 2565
AccB1I GGYRCC 1 cut(s) 2587
AccII CGCG 1 cut(s) 2202
AciI CCGC 2 cut(s) 2200, 2202
AclI AACGTT 1 cut(s) 2279
AclWI GGATC 4 cut(s) 68, 95, 934, 2044
AcoI YGGCCR 2 cut(s) 910, 1892
AcuI CTGAAG 5 cut(s) 491, 1255, 1674, 2055, 2078
AfaI GTAC 9 cut(s) 37, 53, 257, 284, 553, 1574, 1742, 2032, 2613
AfiI CCNNNNNNNGG 5 cut(s) 265, 489, 709, 874, 2729
AflII CTTAAG 1 cut(s) 1505
AflIII ACRYGT 1 cut(s) 1365
AjnI CCWGG 3 cut(s) 201, 1376, 2239
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 3 cut(s) 829, 1145, 1506
AlwI GGATC 4 cut(s) 68, 95, 934, 2044
Ama87I CYCGRG 1 cut(s) 2468
AoxI GGCC 9 cut(s) 199, 208, 700, 845, 881, 910, 1042, 1892, 1974
ApeKI GCWGC 5 cut(s) 155, 323, 524, 2098, 2533
AseI ATTAAT 2 cut(s) 354, 1188
Asp700I GAANNNNTTC 1 cut(s) 1917
AspS9I GGNCC 6 cut(s) 208, 881, 1882, 1974, 2237, 2415
AsuHPI GGTGA 7 cut(s) 776, 857, 992, 1024, 1367, 1545, 1789
AsuII TTCGAA 1 cut(s) 730
AvaI CYCGRG 1 cut(s) 2468
AvaII GGWCC 3 cut(s) 1882, 2237, 2415
BaeGI GKGCMC 1 cut(s) 2590
BalI TGGCCA 1 cut(s) 1894
BanI GGYRCC 1 cut(s) 2587
BanII GRGCYC 2 cut(s) 1377, 1418
BauI CACGAG 1 cut(s) 1046
Bbv12I GWGCWC 1 cut(s) 1053
BbvI GCAGC 5 cut(s) 142, 310, 536, 2085, 2545
BccI CCATC 6 cut(s) 191, 356, 479, 951, 1894, 2405
BcgI CGANNNNNNTGC 2 cut(s) 2045, 2079
BciT130I CCWGG 3 cut(s) 203, 1378, 2241
BciVI GTATCC 3 cut(s) 262, 595, 2207
BclI TGATCA 1 cut(s) 1875
BcoDI GTCTC 3 cut(s) 829, 1145, 1506
BfaI CTAG 2 cut(s) 246, 1910
BfmI CTRYAG 2 cut(s) 324, 2207
BfrI CTTAAG 1 cut(s) 1505
BfuI GTATCC 3 cut(s) 262, 595, 2207
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 1939
BisI GCNGC 5 cut(s) 156, 324, 525, 2099, 2534
BlsI GCNGC 5 cut(s) 157, 325, 526, 2100, 2535
BmcAI AGTACT 1 cut(s) 2032
Bme1390I CCNGG 3 cut(s) 203, 1378, 2241
Bme18I GGWCC 3 cut(s) 1882, 2237, 2415
BmeT110I CYCGRG 1 cut(s) 2468
BmgT120I GGNCC 6 cut(s) 208, 881, 1882, 1974, 2237, 2415
BmiI GGNNCC 6 cut(s) 1417, 1648, 2238, 2416, 2417, 2589
BmrFI CCNGG 3 cut(s) 203, 1378, 2241
BmrI ACTGGG 2 cut(s) 1529, 2660
BmsI GCATC 8 cut(s) 415, 523, 667, 1027, 1348, 1448, 1953, 2657
BmuI ACTGGG 2 cut(s) 1529, 2660
BpmI CTGGAG 5 cut(s) 224, 511, 809, 840, 1402
Bpu10I CCTNAGC 1 cut(s) 1725
Bpu14I TTCGAA 1 cut(s) 730
BpuEI CTTGAG 5 cut(s) 245, 1954, 2375, 2392, 2405
Bsa29I ATCGAT 1 cut(s) 2343
BsaAI YACGTR 2 cut(s) 1195, 1475
BsaBI GATNNNNATC 1 cut(s) 1026
BsaJI CCNNGG 4 cut(s) 162, 1376, 2200, 2469
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 489, 709, 874, 2729
Bse1I ACTGG 5 cut(s) 766, 1244, 1535, 1821, 2666
Bse3DI GCAATG 2 cut(s) 1256, 2332
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 3 cut(s) 203, 1378, 2241
BseCI ATCGAT 1 cut(s) 2343
BseDI CCNNGG 4 cut(s) 162, 1376, 2200, 2469
BseGI GGATG 4 cut(s) 172, 532, 962, 1485
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 5 cut(s) 265, 489, 709, 874, 2729
BseMI GCAATG 2 cut(s) 1256, 2332
BseMII CTCAG 7 cut(s) 149, 282, 407, 1739, 2031, 2519, 2631
BseNI ACTGG 5 cut(s) 766, 1244, 1535, 1821, 2666
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2590
BseXI GCAGC 5 cut(s) 142, 310, 536, 2085, 2545
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 1 cut(s) 2202
BshFI GGCC 9 cut(s) 201, 210, 702, 847, 883, 912, 1044, 1894, 1976
BshNI GGYRCC 1 cut(s) 2587
BshVI ATCGAT 1 cut(s) 2343
BsiHKAI GWGCWC 1 cut(s) 1053
BsiHKCI CYCGRG 1 cut(s) 2468
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 794, 1617, 2428
BslI CCNNNNNNNGG 5 cut(s) 265, 489, 709, 874, 2729
BsmAI GTCTC 3 cut(s) 829, 1145, 1506
BsmFI GGGAC 3 cut(s) 794, 1617, 2428
BsnI GGCC 9 cut(s) 201, 210, 702, 847, 883, 912, 1044, 1894, 1976
BsoBI CYCGRG 1 cut(s) 2468
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 1053, 1377, 1418, 2590
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 2 cut(s) 2200, 2202
BspANI GGCC 9 cut(s) 201, 210, 702, 847, 883, 912, 1044, 1894, 1976
BspCNI CTCAG 7 cut(s) 148, 283, 406, 1738, 2032, 2520, 2632
BspDI ATCGAT 1 cut(s) 2343
BspFNI CGCG 1 cut(s) 2202
BspLI GGNNCC 6 cut(s) 1417, 1648, 2238, 2416, 2417, 2589
BspMAI CTGCAG 2 cut(s) 328, 2211
BspPI GGATC 4 cut(s) 68, 95, 934, 2044
BspT104I TTCGAA 1 cut(s) 730
BspT107I GGYRCC 1 cut(s) 2587
BspTI CTTAAG 1 cut(s) 1505
BsrDI GCAATG 2 cut(s) 1256, 2332
BsrI ACTGG 5 cut(s) 766, 1244, 1535, 1821, 2666
BssECI CCNNGG 4 cut(s) 162, 1376, 2200, 2469
BssSI CACGAG 1 cut(s) 1046
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 1046
Bst2UI CCWGG 3 cut(s) 203, 1378, 2241
Bst4CI ACNGT 5 cut(s) 808, 1740, 1825, 2230, 2683
Bst6I CTCTTC 2 cut(s) 12, 366
BstAFI CTTAAG 1 cut(s) 1505
BstBAI YACGTR 2 cut(s) 1195, 1475
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 3 cut(s) 1042, 1073, 1843
BstDSI CCRYGG 2 cut(s) 162, 2200
BstENI CCTNNNNNAGG 1 cut(s) 707
BstF5I GGATG 4 cut(s) 172, 532, 962, 1485
BstFNI CGCG 1 cut(s) 2202
BstMAI GTCTC 3 cut(s) 829, 1145, 1506
BstMWI GCNNNNNNNGC 7 cut(s) 207, 320, 511, 1303, 1721, 1730, 2359
BstNI CCWGG 3 cut(s) 203, 1378, 2241
BstNSI RCATGY 3 cut(s) 1057, 1369, 2060
BstSCI CCNGG 3 cut(s) 201, 1376, 2239
BstSFI CTRYAG 2 cut(s) 324, 2207
BstSLI GKGCMC 1 cut(s) 2590
BstUI CGCG 1 cut(s) 2202
BstV1I GCAGC 5 cut(s) 142, 310, 536, 2085, 2545
BstX2I RGATCY 2 cut(s) 926, 1939
BstXI CCANNNNNNTGG 1 cut(s) 1643
BstYI RGATCY 2 cut(s) 926, 1939
Bsu15I ATCGAT 1 cut(s) 2343
BsuI GTATCC 3 cut(s) 262, 595, 2207
BsuRI GGCC 9 cut(s) 201, 210, 702, 847, 883, 912, 1044, 1894, 1976
BsuTUI ATCGAT 1 cut(s) 2343
BtgI CCRYGG 2 cut(s) 162, 2200
BtsCI GGATG 4 cut(s) 172, 532, 962, 1485
BtsIMutI CAGTG 6 cut(s) 178, 288, 2235, 2525, 2673, 2679
Cac8I GCNNGC 3 cut(s) 1042, 1073, 1843
Cfr13I GGNCC 6 cut(s) 208, 881, 1882, 1974, 2237, 2415
Cfr42I CCGCGG 1 cut(s) 2203
ClaI ATCGAT 1 cut(s) 2343
Csp6I GTAC 9 cut(s) 36, 52, 256, 283, 552, 1573, 1741, 2031, 2612
CviQI GTAC 9 cut(s) 36, 52, 256, 283, 552, 1573, 1741, 2031, 2612
DraI TTTAAA 2 cut(s) 1015, 1849
EaeI YGGCCR 2 cut(s) 910, 1892
Eam1104I CTCTTC 2 cut(s) 12, 366
EarI CTCTTC 2 cut(s) 12, 366
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 1 cut(s) 702
Eco24I GRGCYC 2 cut(s) 1377, 1418
Eco47I GGWCC 3 cut(s) 1882, 2237, 2415
Eco57I CTGAAG 5 cut(s) 491, 1255, 1674, 2055, 2078
Eco88I CYCGRG 1 cut(s) 2468
EcoNI CCTNNNNNAGG 1 cut(s) 707
EcoO109I RGGNCCY 3 cut(s) 208, 1974, 2415
EcoRI GAATTC 2 cut(s) 1422, 2315
EcoRII CCWGG 3 cut(s) 201, 1376, 2239
EcoT14I CCWWGG 1 cut(s) 162
EcoT22I ATGCAT 2 cut(s) 660, 2650
EcoT38I GRGCYC 2 cut(s) 1377, 1418
ErhI CCWWGG 1 cut(s) 162
FalI AAGNNNNNCTT 4 cut(s) 669, 701, 2732, 2764
FaqI GGGAC 3 cut(s) 794, 1617, 2428
FauNDI CATATG 2 cut(s) 231, 1609
FbaI TGATCA 1 cut(s) 1875
Fnu4HI GCNGC 5 cut(s) 156, 324, 525, 2099, 2534
FokI GGATG 4 cut(s) 179, 519, 969, 1472
FriOI GRGCYC 2 cut(s) 1377, 1418
Fsp4HI GCNGC 5 cut(s) 156, 324, 525, 2099, 2534
FspBI CTAG 2 cut(s) 246, 1910
GluI GCNGC 5 cut(s) 156, 324, 525, 2099, 2534
GsuI CTGGAG 5 cut(s) 224, 511, 809, 840, 1402
HaeIII GGCC 9 cut(s) 201, 210, 702, 847, 883, 912, 1044, 1894, 1976
HindIII AAGCTT 4 cut(s) 1304, 1843, 2291, 2351
HphI GGTGA 7 cut(s) 776, 857, 992, 1024, 1367, 1545, 1789
Hpy166II GTNNAC 1 cut(s) 2612
Hpy8I GTNNAC 1 cut(s) 2612
HpyCH4III ACNGT 5 cut(s) 808, 1740, 1825, 2230, 2683
HpyCH4IV ACGT 3 cut(s) 1194, 1474, 2279
HpyF10VI GCNNNNNNNGC 7 cut(s) 207, 320, 511, 1303, 1721, 1730, 2359
HpySE526I ACGT 3 cut(s) 1194, 1474, 2279
KflI GGGWCCC 1 cut(s) 2415
Ksp22I TGATCA 1 cut(s) 1875
KspI CCGCGG 1 cut(s) 2203
LmnI GCTCC 3 cut(s) 1421, 1652, 2666
Lsp1109I GCAGC 5 cut(s) 142, 310, 536, 2085, 2545
LweI GCATC 8 cut(s) 415, 523, 667, 1027, 1348, 1448, 1953, 2657
MaeI CTAG 2 cut(s) 246, 1910
MaeII ACGT 3 cut(s) 1194, 1474, 2279
MaeIII GTNAC 6 cut(s) 1095, 1551, 1777, 1988, 2159, 2715
MfeI CAATTG 2 cut(s) 559, 1627
MflI RGATCY 2 cut(s) 926, 1939
MhlI GDGCHC 4 cut(s) 1053, 1377, 1418, 2590
MlsI TGGCCA 1 cut(s) 1894
MluNI TGGCCA 1 cut(s) 1894
MlyI GAGTC 3 cut(s) 1346, 1774, 2051
MmeI TCCRAC 1 cut(s) 579
Mox20I TGGCCA 1 cut(s) 1894
Mph1103I ATGCAT 2 cut(s) 660, 2650
MroXI GAANNNNTTC 1 cut(s) 1917
MscI TGGCCA 1 cut(s) 1894
MslI CAYNNNNRTG 4 cut(s) 519, 955, 1641, 2067
Msp20I TGGCCA 1 cut(s) 1894
MspA1I CMGCKG 4 cut(s) 323, 422, 2098, 2202
MspCI CTTAAG 1 cut(s) 1505
MspR9I CCNGG 3 cut(s) 203, 1378, 2241
MunI CAATTG 2 cut(s) 559, 1627
MvaI CCWGG 3 cut(s) 203, 1378, 2241
MvnI CGCG 1 cut(s) 2202
MwoI GCNNNNNNNGC 7 cut(s) 207, 320, 511, 1303, 1721, 1730, 2359
NcoI CCATGG 1 cut(s) 162
NdeI CATATG 2 cut(s) 231, 1609
NlaIV GGNNCC 6 cut(s) 1417, 1648, 2238, 2416, 2417, 2589
NmuCI GTSAC 3 cut(s) 1551, 1777, 2715
NsiI ATGCAT 2 cut(s) 660, 2650
NspI RCATGY 3 cut(s) 1057, 1369, 2060
NspV TTCGAA 1 cut(s) 730
PceI AGGCCT 1 cut(s) 702
PciI ACATGT 1 cut(s) 1365
PdmI GAANNNNTTC 1 cut(s) 1917
PfeI GAWTC 7 cut(s) 455, 529, 793, 983, 989, 1326, 2626
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2239
PkrI GCNGC 5 cut(s) 157, 325, 526, 2100, 2535
PleI GAGTC 3 cut(s) 1345, 1774, 2050
PpsI GAGTC 3 cut(s) 1345, 1774, 2050
Ppu21I YACGTR 2 cut(s) 1195, 1475
PpuMI RGGWCCY 1 cut(s) 2415
PscI ACATGT 1 cut(s) 1365
PshBI ATTAAT 2 cut(s) 354, 1188
PsiI TTATAA 2 cut(s) 1794, 2565
Psp1406I AACGTT 1 cut(s) 2279
Psp5II RGGWCCY 1 cut(s) 2415
Psp6I CCWGG 3 cut(s) 201, 1376, 2239
PspGI CCWGG 3 cut(s) 201, 1376, 2239
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1417, 1648, 2238, 2416, 2417, 2589
PspPI GGNCC 6 cut(s) 208, 881, 1882, 1974, 2237, 2415
PspPPI RGGWCCY 1 cut(s) 2415
PstI CTGCAG 2 cut(s) 328, 2211
PsuI RGATCY 2 cut(s) 926, 1939
PvuII CAGCTG 3 cut(s) 323, 422, 2098
RsaI GTAC 9 cut(s) 37, 53, 257, 284, 553, 1574, 1742, 2032, 2613
RsaNI GTAC 9 cut(s) 36, 52, 256, 283, 552, 1573, 1741, 2031, 2612
RseI CAYNNNNRTG 4 cut(s) 519, 955, 1641, 2067
SacII CCGCGG 1 cut(s) 2203
SatI GCNGC 5 cut(s) 156, 324, 525, 2099, 2534
Sau96I GGNCC 6 cut(s) 208, 881, 1882, 1974, 2237, 2415
ScaI AGTACT 1 cut(s) 2032
SchI GAGTC 3 cut(s) 1346, 1774, 2051
ScrFI CCNGG 3 cut(s) 203, 1378, 2241
SduI GDGCHC 4 cut(s) 1053, 1377, 1418, 2590
SfaNI GCATC 8 cut(s) 415, 523, 667, 1027, 1348, 1448, 1953, 2657
SfcI CTRYAG 2 cut(s) 324, 2207
SfiI GGCCNNNNNGGCC 1 cut(s) 207
Sfr303I CCGCGG 1 cut(s) 2203
SfuI TTCGAA 1 cut(s) 730
SgrBI CCGCGG 1 cut(s) 2203
SinI GGWCC 3 cut(s) 1882, 2237, 2415
SmiMI CAYNNNNRTG 4 cut(s) 519, 955, 1641, 2067
SmlI CTYRAG 6 cut(s) 224, 1505, 1969, 2354, 2371, 2420
SmoI CTYRAG 6 cut(s) 224, 1505, 1969, 2354, 2371, 2420
SseBI AGGCCT 1 cut(s) 702
SsiI CCGC 2 cut(s) 2200, 2202
SspI AATATT 2 cut(s) 1204, 1675
SspMI CTAG 2 cut(s) 246, 1910
StuI AGGCCT 1 cut(s) 702
StyD4I CCNGG 3 cut(s) 201, 1376, 2239
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 5 cut(s) 808, 1740, 1825, 2230, 2683
TaiI ACGT 3 cut(s) 1197, 1477, 2282
TaqI TCGA 7 cut(s) 278, 580, 730, 1371, 2065, 2343, 2691
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 5 cut(s) 51, 282, 551, 1740, 2030
TfiI GAWTC 7 cut(s) 455, 529, 793, 983, 989, 1326, 2626
TscAI CASTG 6 cut(s) 178, 295, 2235, 2532, 2673, 2686
TseFI GTSAC 3 cut(s) 1551, 1777, 2715
TseI GCWGC 5 cut(s) 155, 323, 524, 2098, 2533
Tsp45I GTSAC 3 cut(s) 1551, 1777, 2715
TspGWI ACGGA 2 cut(s) 53, 1508
TspRI CASTG 6 cut(s) 178, 295, 2235, 2532, 2673, 2686
Vha464I CTTAAG 1 cut(s) 1505
VpaK11BI GGWCC 3 cut(s) 1882, 2237, 2415
VspI ATTAAT 2 cut(s) 354, 1188
XagI CCTNNNNNAGG 1 cut(s) 707
XceI RCATGY 3 cut(s) 1057, 1369, 2060
XcmI CCANNNNNNNNNTGG 1 cut(s) 1763
XmnI GAANNNNTTC 1 cut(s) 1917
XspI CTAG 2 cut(s) 246, 1910
ZrmI AGTACT 1 cut(s) 2032
Zsp2I ATGCAT 2 cut(s) 660, 2650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.