Rorug03G0195300

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001584
Physical Location & Seq
Reverse (-)
17182968 .. 17184284
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0195300.1

Sequence Viewer

Length: 726 bp
ATGGCCCCAAGTAATACCATCTCCATGGTTCAAGTCTGTTGTTGCATTCTCAAACCCATTCCTGGAGGGTTTTCATTGCGAAAACTTACTGGATTGGCGAAATGGGTGCTCTTGTGCCGCTGCATAATCTGCCACATTATTTTCTGGTTTGGAGCGTTCGCATTTATTCTCTGGCTGATCTTCTTTCCTCAGGAGCCTAGATTTACTGTTACGAATGCCTCTCTAACCCAATTCAATTTTAACAGTAGTGACAACAACACCGGTGCTAACCTTCACTATCACCTCGCCCTCAACATTACCATCAGAAACCCCAATAGAAGGGTCGGCATTGACTACCGTAGCATGACAGTGTCTGCTAGCTACAGAAAGAAGAAGTTTGGCCTCGTCAATTTGATGGATTCAGTACCATTTTACCAAGGCCACAAGAACACAACTTTTTTGCATCATCTGGTGCTTCAAGGGCAGCAGCAGTTGGTGAAATTCGGGAAGCGTGACATCTCCCAGTTTGACAAGGAGACTGCTGCTGGTGTGTATAGTATTACTGTGCAGCTTACTTTTCGGGTCAAGATCAGGTATGGAAACAATAAGGTCAAGTTTTTGACACCTGTAACCTACGATGACCAGCAGATCAACTGCAAGCTGAAACTTCCTGTAAGTGTTACTAATGAGACATCTGGTGGTGGTTTCAAGGCTACAAAGTGTGGCGATGTTGGTATATTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

241

Amino Acids

27.22

Weight (kDa)

9.78

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 193 9.9e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 118
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 1 cut(s) 405
AfiI CCNNNNNNNGG 2 cut(s) 62, 318
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 4 cut(s) 32, 235, 458, 688
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 3 cut(s) 360, 550, 640
AluI AGCT 3 cut(s) 360, 550, 640
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 2 cut(s) 509, 662
AlwNI CAGNNNCTG 1 cut(s) 353
AoxI GGCC 3 cut(s) 3, 379, 418
ApeKI GCWGC 5 cut(s) 120, 463, 466, 521, 547
ApoI RAATTY 1 cut(s) 479
AsiGI ACCGGT 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 272, 487
AsuNHI GCTAGC 1 cut(s) 356
AxyI CCTNAGG 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 5 cut(s) 107, 475, 478, 508, 559
BccI CCATC 3 cut(s) 26, 308, 388
BcgI CGANNNNNNTGC 2 cut(s) 88, 122
BciT130I CCWGG 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 509, 662
BfaI CTAG 2 cut(s) 198, 357
BfmI CTRYAG 1 cut(s) 361
BisI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 548
BlsI GCNGC 6 cut(s) 119, 122, 465, 468, 523, 549
Bme1390I CCNGG 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 195
BmrFI CCNGG 1 cut(s) 63
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 451
BmtI GCTAGC 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 496
BpmI CTGGAG 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 24, 415
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 318
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 94, 502
Bse21I CCTNAGG 1 cut(s) 189
Bse3DI GCAATG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 24, 415
BseLI CCNNNNNNNGG 2 cut(s) 62, 318
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 2 cut(s) 94, 502
BseXI GCAGC 5 cut(s) 107, 475, 478, 508, 559
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 3 cut(s) 5, 381, 420
BshTI ACCGGT 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 2 cut(s) 62, 318
BsmAI GTCTC 2 cut(s) 509, 662
BsmI GAATGC 2 cut(s) 45, 220
BsnI GGCC 3 cut(s) 5, 381, 420
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 3 cut(s) 177, 567, 627
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 1 cut(s) 118
BspANI GGCC 3 cut(s) 5, 381, 420
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 6, 195
BspOI GCTAGC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 2 cut(s) 94, 502
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 2 cut(s) 24, 415
BssMI GATC 3 cut(s) 177, 567, 627
BssT1I CCWWGG 2 cut(s) 24, 415
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 5 cut(s) 208, 245, 338, 349, 544
BstAPI GCANNNNNTGC 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 358, 638
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 1 cut(s) 24
BstKTI GATC 3 cut(s) 180, 570, 630
BstMAI GTCTC 2 cut(s) 509, 662
BstMBI GATC 3 cut(s) 177, 567, 627
BstMWI GCNNNNNNNGC 2 cut(s) 129, 460
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 361
BstV1I GCAGC 5 cut(s) 107, 475, 478, 508, 559
BstXI CCANNNNNNTGG 1 cut(s) 25
Bsu36I CCTNAGG 1 cut(s) 189
BsuRI GGCC 3 cut(s) 5, 381, 420
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 720
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 2 cut(s) 358, 638
CaiI CAGNNNCTG 1 cut(s) 353
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 404
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 2 cut(s) 25, 343
CviJI RGCY 9 cut(s) 5, 175, 196, 360, 381, 420, 550, 640, 692
CviKI_1 RGCY 9 cut(s) 5, 175, 196, 360, 381, 420, 550, 640, 692
CviQI GTAC 1 cut(s) 404
DdeI CTNAG 1 cut(s) 189
DpnI GATC 3 cut(s) 179, 569, 629
DpnII GATC 3 cut(s) 177, 567, 627
Eco130I CCWWGG 2 cut(s) 24, 415
Eco81I CCTNAGG 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 24, 415
ErhI CCWWGG 2 cut(s) 24, 415
FaeI CATG 2 cut(s) 28, 346
FaiI YATR 6 cut(s) 26, 125, 344, 534, 576, 716
FatI CATG 2 cut(s) 24, 342
Fnu4HI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 548
Fsp4HI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 548
FspBI CTAG 2 cut(s) 198, 357
GluI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 548
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 3 cut(s) 5, 381, 420
HapII CCGG 1 cut(s) 261
Hin1II CATG 2 cut(s) 28, 346
HinfI GANTC 1 cut(s) 398
HpaII CCGG 1 cut(s) 261
HphI GGTGA 2 cut(s) 272, 487
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 3 cut(s) 191, 484, 565
HpyAV CCTTC 2 cut(s) 281, 312
HpyCH4III ACNGT 5 cut(s) 208, 245, 338, 349, 544
HpyCH4V TGCA 5 cut(s) 45, 123, 442, 547, 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 460
HpyF3I CTNAG 1 cut(s) 189
Hsp92II CATG 2 cut(s) 28, 346
Kzo9I GATC 3 cut(s) 177, 567, 627
LmnI GCTCC 2 cut(s) 152, 193
Lsp1109I GCAGC 5 cut(s) 107, 475, 478, 508, 559
LweI GCATC 1 cut(s) 451
MaeI CTAG 2 cut(s) 198, 357
MaeIII GTNAC 5 cut(s) 208, 248, 491, 607, 658
MalI GATC 3 cut(s) 179, 569, 629
MboI GATC 3 cut(s) 177, 567, 627
MboII GAAGA 2 cut(s) 172, 382
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 4 cut(s) 230, 235, 388, 479
MnlI CCTC 6 cut(s) 59, 198, 229, 293, 299, 392
MseI TTAA 2 cut(s) 240, 724
MslI CAYNNNNRTG 2 cut(s) 23, 347
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 1 cut(s) 63
Mva1269I GAATGC 2 cut(s) 45, 220
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 2 cut(s) 129, 460
NcoI CCATGG 1 cut(s) 24
NdeII GATC 3 cut(s) 177, 567, 627
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 2 cut(s) 28, 346
NlaIV GGNNCC 2 cut(s) 6, 195
NmuCI GTSAC 2 cut(s) 248, 491
PctI GAATGC 2 cut(s) 45, 220
PfeI GAWTC 1 cut(s) 398
PflFI GACNNNGTC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 61
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 6 cut(s) 119, 122, 465, 468, 523, 549
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 2 cut(s) 6, 195
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 353
PsyI GACNNNGTC 1 cut(s) 349
RsaI GTAC 1 cut(s) 405
RsaNI GTAC 1 cut(s) 404
RseI CAYNNNNRTG 2 cut(s) 23, 347
SaqAI TTAA 2 cut(s) 240, 724
SatI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 548
Sau3AI GATC 3 cut(s) 177, 567, 627
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 111
SetI ASST 9 cut(s) 273, 285, 362, 552, 575, 591, 607, 614, 642
SfaNI GCATC 1 cut(s) 451
SfcI CTRYAG 1 cut(s) 361
SgrAI CRCCGGYG 1 cut(s) 260
SmiMI CAYNNNNRTG 2 cut(s) 23, 347
Sse9I AATT 4 cut(s) 230, 235, 388, 479
SsiI CCGC 1 cut(s) 118
SspMI CTAG 2 cut(s) 198, 357
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 2 cut(s) 24, 415
TaaI ACNGT 5 cut(s) 208, 245, 338, 349, 544
TasI AATT 4 cut(s) 230, 235, 388, 479
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 2 cut(s) 240, 724
Tru9I TTAA 2 cut(s) 240, 724
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 2 cut(s) 248, 491
TseI GCWGC 5 cut(s) 120, 463, 466, 521, 547
Tsp45I GTSAC 2 cut(s) 248, 491
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 1 cut(s) 354
Tth111I GACNNNGTC 1 cut(s) 349
XapI RAATTY 1 cut(s) 479
XspI CTAG 2 cut(s) 198, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.