pycom14g06090

Belongs to the peptidase M16 family

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
Physical Location & Seq
Forward (+)
5933561 .. 5943527
9967 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06090.18

Sequence Viewer

Length: 2010 bp
ATGGCGGTCGGCGAGGAAGTGGTGCAGGAAATAGTGAAAGCCCGTACGGACAAGAGGCAGTACAGGAGGATCGTCCTCCCGAACTCTCTCGAAGCACTTCTCATCAGCGATCCCGACACTGACAAGTGTGCTGCTTCCATGGATGTCAACGTTGGTTCTTTCAGCGATCCTGATGGCCTGGAGGGCCTTGCTCATTTCCTTGAGCATATGCTGTTTTATGCGAGTGAAAAGTACCCTTTGGAGGATAGTTACTCCAAGTACATCACTGAGCATGGAGGAAAAACAAATGCTTATACAAGTTCTGAGCACACCAACTATTTTTTTGACGTTAATTCTGATGGTTTTGAAGAGGCTTTGGACAGATTTGCTCAGTTCTTTATTAACCCATTGATGTCAGCTGATGCTACAATGAGGGAAATCAAAGCTGTTGATTCTGAGAATCAGAAAAACCTGTTGTCTGATGGATGGAGAATGAACCAGCTCCAGAAACATGTAAGTGCGGCTGACCATCCGTACCATAAATTTAGTACAGGGAATTGGGAAACTTTGGAGGTCCGTCCAAAAGCAAAAGGATTGGATACCAGAGCTGAGCTTATTAAGTTCTATGAACAGTATTATTCTGCTAACGTCATGCATCTTGTCATTTATGGAAAAGAAAGCCTAGATAAAATTCAAGCCACTGTAGAGGACAAATTTAAGGGTATTCGAAACATTGACCGCAGCTGCCTTTGTTTCGGTGGTGAGCCTTGCACATCAGAACATTTACAGATTCTTGTCAAAACTGTCCCAATCAAAGAGGGACATAAGCTGAGACTTGTGTGGCCAATAGCTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTAGTCATCTCATTGGCCACGAAGCAGAGGGGTCTTTGTATGCCGTCTTGAAAACTTTGGGATGGGCAACAGGCTTGGGTGCTGGAGAATCAGATTCGACTTTGGAATTTTCTTTCTTTATAGTAGATATGGATCTCACTGACGCGGGTCATGAGCATATGCAAGACATAGTGGGCTTGCTTTTCAAATACATTTCCCTCTTACACCAGTCGGGTATCTGCAAATGGATTTTTGATGAGCTTTCTGCAGTTTGCGAGACAAAGTTTCATTATCAGGACAAAAATCAGCCTATTAATTATGTCGTCAGCATTTCGCCAAATATGCAGAAATATCCCCCAAGGGACTGGCTAGTAAAATCATCCTTGCCTTCTAAATTCAGTCCAGACACTATCCAAATGGTGCTGAACAAGCTTTCTCCAGAAAATGTCAGAATTTTCTGGGAATCAAAAAAATTTGAAGGTCATACTAACATGACTGAGCCATGGTATGGGACTGCTTATTCCATCGAGAAAATCAGTGGCTCCATGATTCAGGAATGGATAGTATCTTCCCCAAATGAGAATCTGCACCTACCAGCCCATAATGTGTTCATCCCCACGGACTTGTCTCTTAAGAATGATCATGAGAAGGCCAACTGTCCAGTTCTGTTAAGAAAGTCGCCGTGTTCAACACTCTGGTACAAGCCGGATACAATGTTCTTTATTCCTAAGGCTTACGTTAGGATTCATTTCACTTGTCCCCACACAAGTGAATCCCCTGAAACAGAAGTCTTAACTAAAATGTTTACTCGGCTGTTGATGGATTACTTGAATGAATATGCTTACTATGCTGAGGTTGCTGGACTGTTTTACGCAATAACCCAAACAGATAGTGGGTTTCTGGTGACTCTGGTTGGCTATAACCACAAATTAAGAACTTTACTAGAAACTGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGATAGGTTCGCTGTCATTAAGGAAATGCTCATGAAGGAGTACCAAAACTTGAAATTCAAACAGCCTTATGAGCAGGCTATGAATTACACGGCATTAATTCTACGTGATCATTCTTCGTCATGGATGGAAGAACTTGAAGCTCTTCCTCACCTTCAAGTTGAACGATCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling

Protein Analysis

670

Amino Acids

76.82

Weight (kDa)

5.76

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccII CGCG 1 cut(s) 1016
AciI CCGC 4 cut(s) 5, 500, 718, 1016
AclI AACGTT 1 cut(s) 150
AclWI GGATC 4 cut(s) 77, 104, 161, 1011
AcoI YGGCCR 2 cut(s) 821, 886
AcsI RAATTY 9 cut(s) 521, 669, 692, 837, 977, 1244, 1302, 1322, 1889
AfaI GTAC 8 cut(s) 46, 62, 233, 260, 515, 529, 1550, 1877
AfiI CCNNNNNNNGG 3 cut(s) 241, 850, 1358
AflII CTTAAG 1 cut(s) 1481
AflIII ACRYGT 1 cut(s) 490
AjnI CCWGG 1 cut(s) 177
AleI CACNNNNGTG 1 cut(s) 1617
AloI GAACNNNNNNTCC 2 cut(s) 1550, 1582
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 3 cut(s) 805, 1121, 1482
AlwI GGATC 4 cut(s) 77, 104, 161, 1011
AoxI GGCC 6 cut(s) 175, 184, 821, 857, 886, 1500
ApeKI GCWGC 3 cut(s) 131, 720, 723
ApoI RAATTY 9 cut(s) 521, 669, 692, 837, 977, 1244, 1302, 1322, 1889
AseI ATTAAT 2 cut(s) 1164, 1931
Asp700I GAANNNNTTC 2 cut(s) 96, 1977
AspS9I GGNCC 3 cut(s) 184, 553, 857
AsuHPI GGTGA 4 cut(s) 752, 833, 1765, 1976
AsuII TTCGAA 1 cut(s) 706
AvaII GGWCC 1 cut(s) 553
AxyI CCTNAGG 1 cut(s) 1578
BalI TGGCCA 2 cut(s) 823, 888
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 1701
BbvI GCAGC 3 cut(s) 118, 710, 732
BccI CCATC 9 cut(s) 167, 332, 455, 459, 516, 927, 1382, 1663, 1954
BceAI ACGGC 3 cut(s) 899, 1516, 1941
BciT130I CCWGG 1 cut(s) 179
BciVI GTATCC 2 cut(s) 571, 1552
BclI TGATCA 2 cut(s) 1489, 1942
BcoDI GTCTC 3 cut(s) 805, 1121, 1482
BfaI CTAG 3 cut(s) 662, 1220, 1793
BfmI CTRYAG 3 cut(s) 681, 1116, 1800
BfrI CTTAAG 1 cut(s) 1481
BfuI GTATCC 2 cut(s) 571, 1552
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 4 cut(s) 132, 501, 721, 724
BlpI GCTNAGC 1 cut(s) 588
BlsI GCNGC 4 cut(s) 133, 502, 722, 725
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 184, 553, 857
BmiI GGNNCC 1 cut(s) 1393
BmrFI CCNGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 391, 643
BoxI GACNNNNGTC 1 cut(s) 1017
BpmI CTGGAG 5 cut(s) 200, 467, 816, 975, 1272
Bpu10I CCTNAGC 1 cut(s) 1701
Bpu1102I GCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 1 cut(s) 221
BsaAI YACGTR 1 cut(s) 1940
BsaBI GATNNNNATC 1 cut(s) 1002
BsaJI CCNNGG 4 cut(s) 138, 1208, 1352, 1467
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 850, 1358
Bse1I ACTGG 3 cut(s) 1078, 1220, 1511
Bse21I CCTNAGG 1 cut(s) 1578
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 4 cut(s) 138, 1208, 1352, 1467
BseGI GGATG 7 cut(s) 148, 470, 508, 938, 1229, 1461, 1965
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 3 cut(s) 241, 850, 1358
BseMII CTCAG 8 cut(s) 258, 294, 383, 426, 579, 800, 1338, 1692
BseNI ACTGG 3 cut(s) 1078, 1220, 1511
BseXI GCAGC 3 cut(s) 118, 710, 732
BsgI GTGCAG 2 cut(s) 44, 1421
Bsh1236I CGCG 1 cut(s) 1016
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 6 cut(s) 177, 186, 823, 859, 888, 1502
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 1556
BsiWI CGTACG 1 cut(s) 44
BslFI GGGAC 5 cut(s) 770, 813, 1226, 1375, 1593
BslI CCNNNNNNNGG 3 cut(s) 241, 850, 1358
BsmAI GTCTC 3 cut(s) 805, 1121, 1482
BsmFI GGGAC 5 cut(s) 770, 813, 1226, 1375, 1593
BsnI GGCC 6 cut(s) 177, 186, 823, 859, 888, 1502
Bsp119I TTCGAA 1 cut(s) 706
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
Bsp1720I GCTNAGC 1 cut(s) 588
Bsp19I CCATGG 2 cut(s) 138, 1352
BspACI CCGC 4 cut(s) 5, 500, 718, 1016
BspANI GGCC 6 cut(s) 177, 186, 823, 859, 888, 1502
BspCNI CTCAG 8 cut(s) 259, 295, 382, 427, 580, 801, 1339, 1693
BspFNI CGCG 1 cut(s) 1016
BspHI TCATGA 3 cut(s) 1021, 1492, 1866
BspLI GGNNCC 1 cut(s) 1393
BspMAI CTGCAG 1 cut(s) 1120
BspPI GGATC 4 cut(s) 77, 104, 161, 1011
BspQI GCTCTTC 1 cut(s) 1983
BspT104I TTCGAA 1 cut(s) 706
BspTI CTTAAG 1 cut(s) 1481
BsrI ACTGG 3 cut(s) 1078, 1220, 1511
BssECI CCNNGG 4 cut(s) 138, 1208, 1352, 1467
BssMI GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
BssT1I CCWWGG 3 cut(s) 138, 1208, 1352
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 6 cut(s) 612, 682, 784, 1508, 1716, 1801
Bst6I CTCTTC 2 cut(s) 342, 1983
BstAFI CTTAAG 1 cut(s) 1481
BstBAI YACGTR 1 cut(s) 1940
BstBI TTCGAA 1 cut(s) 706
BstC8I GCNNGC 3 cut(s) 1049, 1819, 1911
BstDSI CCRYGG 3 cut(s) 138, 1352, 1467
BstF5I GGATG 7 cut(s) 148, 470, 508, 938, 1229, 1461, 1965
BstFNI CGCG 1 cut(s) 1016
BstKTI GATC 7 cut(s) 72, 112, 169, 1006, 1492, 1945, 2003
BstMAI GTCTC 3 cut(s) 805, 1121, 1482
BstMBI GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 6 cut(s) 183, 1192, 1279, 1697, 1706, 1906
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 494
BstPAI GACNNNNGTC 1 cut(s) 1017
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 3 cut(s) 681, 1116, 1800
BstUI CGCG 1 cut(s) 1016
BstV1I GCAGC 3 cut(s) 118, 710, 732
BstX2I RGATCY 1 cut(s) 1003
BstXI CCANNNNNNTGG 1 cut(s) 1215
BstYI RGATCY 1 cut(s) 1003
Bsu36I CCTNAGG 1 cut(s) 1578
BsuI GTATCC 2 cut(s) 571, 1552
BsuRI GGCC 6 cut(s) 177, 186, 823, 859, 888, 1502
BtgI CCRYGG 3 cut(s) 138, 1352, 1467
BtsCI GGATG 7 cut(s) 148, 470, 508, 938, 1229, 1461, 1965
BtsIMutI CAGTG 5 cut(s) 117, 264, 678, 1008, 1393
Cac8I GCNNGC 3 cut(s) 1049, 1819, 1911
CciI TCATGA 3 cut(s) 1021, 1492, 1866
Cfr13I GGNCC 3 cut(s) 184, 553, 857
CseI GACGC 1 cut(s) 1022
Csp6I GTAC 8 cut(s) 45, 61, 232, 259, 514, 528, 1549, 1876
CviQI GTAC 8 cut(s) 45, 61, 232, 259, 514, 528, 1549, 1876
DpnI GATC 7 cut(s) 71, 111, 168, 1005, 1491, 1944, 2002
DpnII GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
DraI TTTAAA 1 cut(s) 1825
EaeI YGGCCR 2 cut(s) 821, 886
Eam1104I CTCTTC 2 cut(s) 342, 1983
EarI CTCTTC 2 cut(s) 342, 1983
Eco130I CCWWGG 3 cut(s) 138, 1208, 1352
Eco47I GGWCC 1 cut(s) 553
Eco81I CCTNAGG 1 cut(s) 1578
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 3 cut(s) 138, 1208, 1352
EcoT22I ATGCAT 1 cut(s) 636
ErhI CCWWGG 3 cut(s) 138, 1208, 1352
FaqI GGGAC 5 cut(s) 770, 813, 1226, 1375, 1593
FauI CCCGC 1 cut(s) 1009
FauNDI CATATG 2 cut(s) 207, 1029
FbaI TGATCA 2 cut(s) 1489, 1942
Fnu4HI GCNGC 4 cut(s) 132, 501, 721, 724
FokI GGATG 7 cut(s) 155, 477, 495, 945, 1216, 1448, 1972
Fsp4HI GCNGC 4 cut(s) 132, 501, 721, 724
FspBI CTAG 3 cut(s) 662, 1220, 1793
GluI GCNGC 4 cut(s) 132, 501, 721, 724
GsuI CTGGAG 5 cut(s) 200, 467, 816, 975, 1272
HaeIII GGCC 6 cut(s) 177, 186, 823, 859, 888, 1502
HapII CCGG 1 cut(s) 1556
HgaI GACGC 1 cut(s) 1022
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 1280, 1819
HpaII CCGG 1 cut(s) 1556
HphI GGTGA 4 cut(s) 752, 833, 1765, 1976
Hpy166II GTNNAC 2 cut(s) 148, 1656
Hpy188I TCNGA 8 cut(s) 304, 337, 436, 444, 460, 757, 964, 1301
Hpy8I GTNNAC 2 cut(s) 148, 1656
HpyAV CCTTC 6 cut(s) 848, 1248, 1322, 1492, 1864, 1997
HpyCH4III ACNGT 6 cut(s) 612, 682, 784, 1508, 1716, 1801
HpyCH4IV ACGT 5 cut(s) 150, 327, 627, 1587, 1939
HpyCH4V TGCA 9 cut(s) 25, 634, 750, 1033, 1092, 1118, 1195, 1438, 1817
HpyF10VI GCNNNNNNNGC 6 cut(s) 183, 1192, 1279, 1697, 1706, 1906
HpySE526I ACGT 5 cut(s) 150, 327, 627, 1587, 1939
Ksp22I TGATCA 2 cut(s) 1489, 1942
Kzo9I GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
LguI GCTCTTC 1 cut(s) 1983
LmnI GCTCC 3 cut(s) 486, 835, 1397
Lsp1109I GCAGC 3 cut(s) 118, 710, 732
LweI GCATC 2 cut(s) 391, 643
MaeI CTAG 3 cut(s) 662, 1220, 1793
MaeII ACGT 5 cut(s) 150, 327, 627, 1587, 1939
MaeIII GTNAC 2 cut(s) 248, 1753
MalI GATC 7 cut(s) 71, 111, 168, 1005, 1491, 1944, 2002
MboI GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
MboII GAAGA 6 cut(s) 359, 1410, 1822, 1941, 1970, 1976
MflI RGATCY 1 cut(s) 1003
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 2 cut(s) 823, 888
MluNI TGGCCA 2 cut(s) 823, 888
MlyI GAGTC 1 cut(s) 1750
Mox20I TGGCCA 2 cut(s) 823, 888
Mph1103I ATGCAT 1 cut(s) 636
MroXI GAANNNNTTC 2 cut(s) 96, 1977
MscI TGGCCA 2 cut(s) 823, 888
MslI CAYNNNNRTG 2 cut(s) 495, 1617
Msp20I TGGCCA 2 cut(s) 823, 888
MspA1I CMGCKG 2 cut(s) 398, 723
MspCI CTTAAG 1 cut(s) 1481
MspI CCGG 1 cut(s) 1556
MspR9I CCNGG 1 cut(s) 179
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 1 cut(s) 1016
MwoI GCNNNNNNNGC 6 cut(s) 183, 1192, 1279, 1697, 1706, 1906
NcoI CCATGG 2 cut(s) 138, 1352
NdeI CATATG 2 cut(s) 207, 1029
NdeII GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
NlaIV GGNNCC 1 cut(s) 1393
NmeAIII GCCGAG 1 cut(s) 1639
NmuCI GTSAC 1 cut(s) 1753
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 1 cut(s) 706
OliI CACNNNNGTG 1 cut(s) 1617
PagI TCATGA 3 cut(s) 1021, 1492, 1866
PciI ACATGT 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 1983
PdmI GAANNNNTTC 2 cut(s) 96, 1977
Pfl23II CGTACG 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 1358
PkrI GCNGC 4 cut(s) 133, 502, 722, 725
PleI GAGTC 1 cut(s) 1750
PpsI GAGTC 1 cut(s) 1750
Ppu21I YACGTR 1 cut(s) 1940
PscI ACATGT 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 1017
PshBI ATTAAT 2 cut(s) 1164, 1931
Psp1406I AACGTT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspLI CGTACG 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 1393
PspPI GGNCC 3 cut(s) 184, 553, 857
PstI CTGCAG 1 cut(s) 1120
PsuI RGATCY 1 cut(s) 1003
PvuII CAGCTG 2 cut(s) 398, 723
RsaI GTAC 8 cut(s) 46, 62, 233, 260, 515, 529, 1550, 1877
RsaNI GTAC 8 cut(s) 45, 61, 232, 259, 514, 528, 1549, 1876
RseI CAYNNNNRTG 2 cut(s) 495, 1617
SapI GCTCTTC 1 cut(s) 1983
SatI GCNGC 4 cut(s) 132, 501, 721, 724
Sau3AI GATC 7 cut(s) 69, 109, 166, 1003, 1489, 1942, 2000
Sau96I GGNCC 3 cut(s) 184, 553, 857
SchI GAGTC 1 cut(s) 1750
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 2 cut(s) 391, 643
SfcI CTRYAG 3 cut(s) 681, 1116, 1800
SfiI GGCCNNNNNGGCC 1 cut(s) 183
SfuI TTCGAA 1 cut(s) 706
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 495, 1617
SmlI CTYRAG 2 cut(s) 200, 1481
SmoI CTYRAG 2 cut(s) 200, 1481
SsiI CCGC 4 cut(s) 5, 500, 718, 1016
SspMI CTAG 3 cut(s) 662, 1220, 1793
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 3 cut(s) 138, 1208, 1352
TaaI ACNGT 6 cut(s) 612, 682, 784, 1508, 1716, 1801
TaiI ACGT 5 cut(s) 153, 330, 630, 1590, 1942
TaqI TCGA 4 cut(s) 90, 706, 968, 1377
TatI WGTACW 3 cut(s) 60, 258, 527
TauI GCSGC 1 cut(s) 503
TscAI CASTG 5 cut(s) 124, 271, 685, 1015, 1393
TseFI GTSAC 1 cut(s) 1753
TseI GCWGC 3 cut(s) 131, 720, 723
Tsp45I GTSAC 1 cut(s) 1753
TspDTI ATGAA 8 cut(s) 488, 621, 1127, 1450, 1586, 1698, 1883, 1931
TspGWI ACGGA 4 cut(s) 62, 501, 545, 1484
TspRI CASTG 5 cut(s) 124, 271, 685, 1015, 1393
Van91I CCANNNNNTGG 1 cut(s) 1358
Vha464I CTTAAG 1 cut(s) 1481
VpaK11BI GGWCC 1 cut(s) 553
VspI ATTAAT 2 cut(s) 1164, 1931
XapI RAATTY 9 cut(s) 521, 669, 692, 837, 977, 1244, 1302, 1322, 1889
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 1739
XmnI GAANNNNTTC 2 cut(s) 96, 1977
XspI CTAG 3 cut(s) 662, 1220, 1793
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.