Rh3CG277400

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001584
Physical Location & Seq
Forward (+)
27674993 .. 27720904
45912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG277400.1

Sequence Viewer

Length: 2277 bp
ATGCATCTGGTTATATATGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTCCCAATAAAAGACGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGAGCACATGCGAGATATAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCAGGTGTCTGCAAATGGATATTTGATGAGCTATCTGCTGTTTGCGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTGGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGACATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCAGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATGCAAACATGTTCGAGCCCTGGTATGAAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATGAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCAACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGCCCAGTTCTGTTAAGAAAGTCACCATATACAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATATGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGAAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCTCAGGTTGCTGAACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGATTCTTGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGGAGTACCAAAACTACAAATTTCAACAGCCTTATGAGCAGGCTATGTACTATTGCTCATTAATTCTACAGGACCAGACTTGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCAATGATGCTCTCAAGGGCCTTCTTAGAGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCCATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGAGATGAGAATTCTTCCCTCGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCAGCTGCTTGTTCTTATTGCAAAGCAACCAGCCTTCCACCAGCTTAGATCAGTTGAGCAACTTGGTTACATAACTGCTCTTTTACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGAAAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAGCTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

758

Amino Acids

87.86

Weight (kDa)

6.38

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 1 - 161 6.9e-22 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 169 - 448 2.1e-102 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 453 - 639 4.7e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 561 - 659 6.5e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1140, 2073
AccB1I GGYRCC 1 cut(s) 2095
AccII CGCG 1 cut(s) 1782
AciI CCGC 2 cut(s) 1780, 1782
AclI AACGTT 1 cut(s) 1859
AclWI GGATC 2 cut(s) 280, 1456
AcoI YGGCCR 2 cut(s) 256, 1322
AcuI CTGAAG 4 cut(s) 601, 1020, 1467, 1658
AfaI GTAC 6 cut(s) 920, 1088, 1247, 1289, 1444, 2121
AfiI CCNNNNNNNGG 4 cut(s) 55, 220, 1589, 2237
AflII CTTAAG 1 cut(s) 851
AflIII ACRYGT 1 cut(s) 711
AjnI CCWGG 3 cut(s) 722, 1635, 1819
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 3 cut(s) 175, 491, 852
AlwI GGATC 2 cut(s) 280, 1456
AoxI GGCC 8 cut(s) 46, 191, 227, 256, 388, 1322, 1404, 1591
ApeKI GCWGC 3 cut(s) 1678, 2030, 2041
AseI ATTAAT 2 cut(s) 534, 1301
Asp700I GAANNNNTTC 1 cut(s) 1347
AspS9I GGNCC 5 cut(s) 227, 1312, 1404, 1817, 1995
AsuHPI GGTGA 7 cut(s) 122, 203, 338, 370, 713, 891, 1135
AsuII TTCGAA 1 cut(s) 76
AvaII GGWCC 3 cut(s) 1312, 1817, 1995
BaeGI GKGCMC 1 cut(s) 2098
BalI TGGCCA 1 cut(s) 1324
BanI GGYRCC 1 cut(s) 2095
BanII GRGCYC 2 cut(s) 723, 764
BauI CACGAG 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 1665, 2042, 2053
BccI CCATC 4 cut(s) 297, 1324, 1531, 1985
BciT130I CCWGG 3 cut(s) 724, 1637, 1821
BciVI GTATCC 1 cut(s) 1787
BclI TGATCA 1 cut(s) 1221
BcoDI GTCTC 3 cut(s) 175, 491, 852
BfaI CTAG 1 cut(s) 1340
BfmI CTRYAG 2 cut(s) 1307, 1787
BfrI CTTAAG 1 cut(s) 851
BfuI GTATCC 1 cut(s) 1787
BglII AGATCT 1 cut(s) 1369
BisI GCNGC 3 cut(s) 1679, 2031, 2042
BlsI GCNGC 3 cut(s) 1680, 2032, 2043
BmcAI AGTACT 1 cut(s) 1444
Bme1390I CCNGG 3 cut(s) 724, 1637, 1821
Bme18I GGWCC 3 cut(s) 1312, 1817, 1995
BmgT120I GGNCC 5 cut(s) 227, 1312, 1404, 1817, 1995
BmiI GGNNCC 6 cut(s) 763, 994, 1818, 1996, 1997, 2097
BmrFI CCNGG 3 cut(s) 724, 1637, 1821
BmrI ACTGGG 2 cut(s) 875, 2168
BmsI GCATC 6 cut(s) 13, 373, 694, 794, 1383, 2165
BmuI ACTGGG 2 cut(s) 875, 2168
BpmI CTGGAG 3 cut(s) 155, 186, 748
Bpu10I CCTNAGC 1 cut(s) 1071
Bpu14I TTCGAA 1 cut(s) 76
BpuEI CTTGAG 4 cut(s) 1384, 1955, 1972, 1985
Bsa29I ATCGAT 2 cut(s) 1923, 2199
BsaAI YACGTR 3 cut(s) 541, 821, 1471
BsaBI GATNNNNATC 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 722, 1780
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 220, 1589, 2237
Bse1I ACTGG 5 cut(s) 112, 590, 881, 1167, 2174
Bse3DI GCAATG 2 cut(s) 602, 1912
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 3 cut(s) 724, 1637, 1821
BseCI ATCGAT 2 cut(s) 1923, 2199
BseDI CCNNGG 2 cut(s) 722, 1780
BseGI GGATG 3 cut(s) 308, 831, 1523
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 4 cut(s) 55, 220, 1589, 2237
BseMI GCAATG 2 cut(s) 602, 1912
BseMII CTCAG 4 cut(s) 1085, 1443, 2027, 2139
BseNI ACTGG 5 cut(s) 112, 590, 881, 1167, 2174
BseSI GKGCMC 1 cut(s) 2098
BseXI GCAGC 3 cut(s) 1665, 2042, 2053
BsgI GTGCAG 1 cut(s) 1451
Bsh1236I CGCG 1 cut(s) 1782
BshFI GGCC 8 cut(s) 48, 193, 229, 258, 390, 1324, 1406, 1593
BshNI GGYRCC 1 cut(s) 2095
BshVI ATCGAT 2 cut(s) 1923, 2199
BsiHKAI GWGCWC 1 cut(s) 399
BslFI GGGAC 3 cut(s) 140, 963, 2008
BslI CCNNNNNNNGG 4 cut(s) 55, 220, 1589, 2237
BsmAI GTCTC 3 cut(s) 175, 491, 852
BsmFI GGGAC 3 cut(s) 140, 963, 2008
BsnI GGCC 8 cut(s) 48, 193, 229, 258, 390, 1324, 1406, 1593
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 4 cut(s) 399, 723, 764, 2098
Bsp143I GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
BspACI CCGC 2 cut(s) 1780, 1782
BspANI GGCC 8 cut(s) 48, 193, 229, 258, 390, 1324, 1406, 1593
BspCNI CTCAG 4 cut(s) 1084, 1444, 2028, 2140
BspDI ATCGAT 2 cut(s) 1923, 2199
BspFNI CGCG 1 cut(s) 1782
BspLI GGNNCC 6 cut(s) 763, 994, 1818, 1996, 1997, 2097
BspMAI CTGCAG 1 cut(s) 1791
BspPI GGATC 2 cut(s) 280, 1456
BspT104I TTCGAA 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 2095
BspTI CTTAAG 1 cut(s) 851
BsrDI GCAATG 2 cut(s) 602, 1912
BsrI ACTGG 5 cut(s) 112, 590, 881, 1167, 2174
BssECI CCNNGG 2 cut(s) 722, 1780
BssMI GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
BssSI CACGAG 1 cut(s) 392
Bst2BI CACGAG 1 cut(s) 392
Bst2UI CCWGG 3 cut(s) 724, 1637, 1821
Bst4CI ACNGT 6 cut(s) 154, 170, 1086, 1171, 1810, 2191
BstAFI CTTAAG 1 cut(s) 851
BstAPI GCANNNNNTGC 1 cut(s) 2027
BstBAI YACGTR 3 cut(s) 541, 821, 1471
BstBI TTCGAA 1 cut(s) 76
BstC8I GCNNGC 4 cut(s) 388, 419, 1189, 1281
BstDEI CTNAG 7 cut(s) 1071, 1377, 1411, 1452, 1718, 2036, 2148
BstDSI CCRYGG 1 cut(s) 1780
BstENI CCTNNNNNAGG 2 cut(s) 53, 1587
BstF5I GGATG 3 cut(s) 308, 831, 1523
BstFNI CGCG 1 cut(s) 1782
BstKTI GATC 9 cut(s) 275, 376, 599, 1224, 1372, 1464, 1724, 1923, 2011
BstMAI GTCTC 3 cut(s) 175, 491, 852
BstMBI GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
BstMWI GCNNNNNNNGC 6 cut(s) 649, 1067, 1076, 1276, 1939, 2027
BstNI CCWGG 3 cut(s) 724, 1637, 1821
BstNSI RCATGY 2 cut(s) 403, 715
BstSCI CCNGG 3 cut(s) 722, 1635, 1819
BstSFI CTRYAG 2 cut(s) 1307, 1787
BstSLI GKGCMC 1 cut(s) 2098
BstUI CGCG 1 cut(s) 1782
BstV1I GCAGC 3 cut(s) 1665, 2042, 2053
BstX2I RGATCY 2 cut(s) 272, 1369
BstXI CCANNNNNNTGG 1 cut(s) 989
BstYI RGATCY 2 cut(s) 272, 1369
Bsu15I ATCGAT 2 cut(s) 1923, 2199
BsuI GTATCC 1 cut(s) 1787
BsuRI GGCC 8 cut(s) 48, 193, 229, 258, 390, 1324, 1406, 1593
BsuTUI ATCGAT 2 cut(s) 1923, 2199
BtgI CCRYGG 1 cut(s) 1780
BtsCI GGATG 3 cut(s) 308, 831, 1523
BtsIMutI CAGTG 4 cut(s) 1815, 2033, 2181, 2187
Cac8I GCNNGC 4 cut(s) 388, 419, 1189, 1281
Cfr13I GGNCC 5 cut(s) 227, 1312, 1404, 1817, 1995
Cfr42I CCGCGG 1 cut(s) 1783
ClaI ATCGAT 2 cut(s) 1923, 2199
Csp6I GTAC 6 cut(s) 919, 1087, 1246, 1288, 1443, 2120
CviAII CATG 8 cut(s) 189, 231, 260, 400, 712, 745, 983, 2264
CviQI GTAC 6 cut(s) 919, 1087, 1246, 1288, 1443, 2120
DdeI CTNAG 7 cut(s) 1071, 1377, 1411, 1452, 1718, 2036, 2148
DpnI GATC 9 cut(s) 274, 375, 598, 1223, 1371, 1463, 1723, 1922, 2010
DpnII GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
DraI TTTAAA 2 cut(s) 361, 1195
EaeI YGGCCR 2 cut(s) 256, 1322
Eco147I AGGCCT 2 cut(s) 48, 1593
Eco24I GRGCYC 2 cut(s) 723, 764
Eco47I GGWCC 3 cut(s) 1312, 1817, 1995
Eco57I CTGAAG 4 cut(s) 601, 1020, 1467, 1658
EcoNI CCTNNNNNAGG 2 cut(s) 53, 1587
EcoO109I RGGNCCY 2 cut(s) 1404, 1995
EcoRI GAATTC 3 cut(s) 768, 1612, 1895
EcoRII CCWGG 3 cut(s) 722, 1635, 1819
EcoT22I ATGCAT 2 cut(s) 6, 2158
EcoT38I GRGCYC 2 cut(s) 723, 764
FaeI CATG 8 cut(s) 192, 234, 263, 403, 715, 748, 986, 2267
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 2240, 2272
FaqI GGGAC 3 cut(s) 140, 963, 2008
FatI CATG 8 cut(s) 188, 230, 259, 399, 711, 744, 982, 2263
FauNDI CATATG 1 cut(s) 955
FbaI TGATCA 1 cut(s) 1221
Fnu4HI GCNGC 3 cut(s) 1679, 2031, 2042
FokI GGATG 3 cut(s) 315, 818, 1510
FriOI GRGCYC 2 cut(s) 723, 764
Fsp4HI GCNGC 3 cut(s) 1679, 2031, 2042
FspBI CTAG 1 cut(s) 1340
GluI GCNGC 3 cut(s) 1679, 2031, 2042
GsuI CTGGAG 3 cut(s) 155, 186, 748
HaeIII GGCC 8 cut(s) 48, 193, 229, 258, 390, 1324, 1406, 1593
Hin1II CATG 8 cut(s) 192, 234, 263, 403, 715, 748, 986, 2267
HindIII AAGCTT 5 cut(s) 650, 1189, 1553, 1871, 1931
HinfI GANTC 9 cut(s) 139, 329, 335, 672, 683, 1126, 1454, 1545, 2134
HphI GGTGA 7 cut(s) 122, 203, 338, 370, 713, 891, 1135
Hpy166II GTNNAC 2 cut(s) 1627, 2120
Hpy188I TCNGA 8 cut(s) 127, 149, 334, 671, 1990, 2149, 2161, 2219
Hpy8I GTNNAC 2 cut(s) 1627, 2120
HpyCH4III ACNGT 6 cut(s) 154, 170, 1086, 1171, 1810, 2191
HpyCH4IV ACGT 4 cut(s) 540, 820, 1470, 1859
HpyF10VI GCNNNNNNNGC 6 cut(s) 649, 1067, 1076, 1276, 1939, 2027
HpyF3I CTNAG 7 cut(s) 1071, 1377, 1411, 1452, 1718, 2036, 2148
HpySE526I ACGT 4 cut(s) 540, 820, 1470, 1859
Hsp92II CATG 8 cut(s) 192, 234, 263, 403, 715, 748, 986, 2267
KflI GGGWCCC 1 cut(s) 1995
Ksp22I TGATCA 1 cut(s) 1221
KspI CCGCGG 1 cut(s) 1783
Kzo9I GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
LmnI GCTCC 3 cut(s) 767, 998, 2174
Lsp1109I GCAGC 3 cut(s) 1665, 2042, 2053
LweI GCATC 6 cut(s) 13, 373, 694, 794, 1383, 2165
MaeI CTAG 1 cut(s) 1340
MaeII ACGT 4 cut(s) 540, 820, 1470, 1859
MaeIII GTNAC 6 cut(s) 441, 897, 1234, 1418, 1739, 2223
MalI GATC 9 cut(s) 274, 375, 598, 1223, 1371, 1463, 1723, 1922, 2010
MboI GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
MboII GAAGA 8 cut(s) 667, 780, 1192, 1340, 1379, 1487, 1608, 1970
MfeI CAATTG 1 cut(s) 973
MflI RGATCY 2 cut(s) 272, 1369
MhlI GDGCHC 4 cut(s) 399, 723, 764, 2098
MlsI TGGCCA 1 cut(s) 1324
MluNI TGGCCA 1 cut(s) 1324
MlyI GAGTC 3 cut(s) 692, 1463, 1554
Mox20I TGGCCA 1 cut(s) 1324
Mph1103I ATGCAT 2 cut(s) 6, 2158
MroXI GAANNNNTTC 1 cut(s) 1347
MscI TGGCCA 1 cut(s) 1324
MslI CAYNNNNRTG 3 cut(s) 301, 987, 1647
Msp20I TGGCCA 1 cut(s) 1324
MspA1I CMGCKG 2 cut(s) 1678, 1782
MspCI CTTAAG 1 cut(s) 851
MspR9I CCNGG 3 cut(s) 724, 1637, 1821
MunI CAATTG 1 cut(s) 973
MvaI CCWGG 3 cut(s) 724, 1637, 1821
MvnI CGCG 1 cut(s) 1782
MwoI GCNNNNNNNGC 6 cut(s) 649, 1067, 1076, 1276, 1939, 2027
NdeI CATATG 1 cut(s) 955
NdeII GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
NlaIII CATG 8 cut(s) 192, 234, 263, 403, 715, 748, 986, 2267
NlaIV GGNNCC 6 cut(s) 763, 994, 1818, 1996, 1997, 2097
NmuCI GTSAC 3 cut(s) 897, 1234, 2223
NsiI ATGCAT 2 cut(s) 6, 2158
NspI RCATGY 2 cut(s) 403, 715
NspV TTCGAA 1 cut(s) 76
PceI AGGCCT 2 cut(s) 48, 1593
PciI ACATGT 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 1347
PfeI GAWTC 6 cut(s) 139, 329, 335, 672, 1126, 2134
PfoI TCCNGGA 1 cut(s) 1819
PkrI GCNGC 3 cut(s) 1680, 2032, 2043
PleI GAGTC 3 cut(s) 691, 1462, 1553
PpsI GAGTC 3 cut(s) 691, 1462, 1553
Ppu21I YACGTR 3 cut(s) 541, 821, 1471
PpuMI RGGWCCY 1 cut(s) 1995
PscI ACATGT 1 cut(s) 711
PshBI ATTAAT 2 cut(s) 534, 1301
PsiI TTATAA 2 cut(s) 1140, 2073
Psp1406I AACGTT 1 cut(s) 1859
Psp5II RGGWCCY 1 cut(s) 1995
Psp6I CCWGG 3 cut(s) 722, 1635, 1819
PspGI CCWGG 3 cut(s) 722, 1635, 1819
PspN4I GGNNCC 6 cut(s) 763, 994, 1818, 1996, 1997, 2097
PspPI GGNCC 5 cut(s) 227, 1312, 1404, 1817, 1995
PspPPI RGGWCCY 1 cut(s) 1995
PstI CTGCAG 1 cut(s) 1791
PsuI RGATCY 2 cut(s) 272, 1369
PvuII CAGCTG 1 cut(s) 1678
RsaI GTAC 6 cut(s) 920, 1088, 1247, 1289, 1444, 2121
RsaNI GTAC 6 cut(s) 919, 1087, 1246, 1288, 1443, 2120
RseI CAYNNNNRTG 3 cut(s) 301, 987, 1647
SacII CCGCGG 1 cut(s) 1783
SatI GCNGC 3 cut(s) 1679, 2031, 2042
Sau3AI GATC 9 cut(s) 272, 373, 596, 1221, 1369, 1461, 1721, 1920, 2008
Sau96I GGNCC 5 cut(s) 227, 1312, 1404, 1817, 1995
ScaI AGTACT 1 cut(s) 1444
SchI GAGTC 3 cut(s) 692, 1463, 1554
ScrFI CCNGG 3 cut(s) 724, 1637, 1821
SduI GDGCHC 4 cut(s) 399, 723, 764, 2098
SfaNI GCATC 6 cut(s) 13, 373, 694, 794, 1383, 2165
SfcI CTRYAG 2 cut(s) 1307, 1787
Sfr303I CCGCGG 1 cut(s) 1783
SfuI TTCGAA 1 cut(s) 76
SgrBI CCGCGG 1 cut(s) 1783
SinI GGWCC 3 cut(s) 1312, 1817, 1995
SmiMI CAYNNNNRTG 3 cut(s) 301, 987, 1647
SmlI CTYRAG 5 cut(s) 851, 1399, 1934, 1951, 2000
SmoI CTYRAG 5 cut(s) 851, 1399, 1934, 1951, 2000
SseBI AGGCCT 2 cut(s) 48, 1593
SsiI CCGC 2 cut(s) 1780, 1782
SspI AATATT 2 cut(s) 550, 1021
SspMI CTAG 1 cut(s) 1340
StuI AGGCCT 2 cut(s) 48, 1593
StyD4I CCNGG 3 cut(s) 722, 1635, 1819
TaaI ACNGT 6 cut(s) 154, 170, 1086, 1171, 1810, 2191
TaiI ACGT 4 cut(s) 543, 823, 1473, 1862
TaqI TCGA 5 cut(s) 76, 717, 1645, 1923, 2199
TaqII GACCGA 1 cut(s) 102
TatI WGTACW 3 cut(s) 1086, 1287, 1442
TfiI GAWTC 6 cut(s) 139, 329, 335, 672, 1126, 2134
TscAI CASTG 4 cut(s) 1815, 2040, 2181, 2194
TseFI GTSAC 3 cut(s) 897, 1234, 2223
TseI GCWGC 3 cut(s) 1678, 2030, 2041
Tsp45I GTSAC 3 cut(s) 897, 1234, 2223
TspGWI ACGGA 1 cut(s) 854
TspRI CASTG 4 cut(s) 1815, 2040, 2181, 2194
Vha464I CTTAAG 1 cut(s) 851
VpaK11BI GGWCC 3 cut(s) 1312, 1817, 1995
VspI ATTAAT 2 cut(s) 534, 1301
XagI CCTNNNNNAGG 2 cut(s) 53, 1587
XceI RCATGY 2 cut(s) 403, 715
XcmI CCANNNNNNNNNTGG 1 cut(s) 1109
XmnI GAANNNNTTC 1 cut(s) 1347
XspI CTAG 1 cut(s) 1340
ZrmI AGTACT 1 cut(s) 1444
Zsp2I ATGCAT 2 cut(s) 6, 2158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.