MD12G1073300.v1.1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001584
Physical Location & Seq
Forward (+)
8778726 .. 8795418
16693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1073300.v1.1.491

Sequence Viewer

Length: 2901 bp
ATGGCGGTCGGGAAGGAAGAGGTGGAGGAAATAGTGAAACCCCGTACGGACAAGAGGGAGTACAGGAGGATTGTCCTAGCCAACTCCCTCGAAATCCTTCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAATGTTGGTGCTTTTAGCGACCCTGATGGCCTGGAGGGTCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAAAAGTACCCTTTGGAGGATAGTTACTCGAAGTACATCACCGAGCATGGAGGGCGAACAAATGCTTATACAGACTCTGAGCACACCAACTATTATTTTGACATCAATTCCGATGGTTTTGAAGAGGCTTTGGACAGATTTGCTCAGTTCTTTATTAAACCATTGATGTCAGCTGAAGCTACAATGAGGGAGATCAAAGCTGTTGATTCTGAGAATCAGAAAAACCTGTTGTCTGATGCATGGAGAATGAATCAGCTCCAGAAACATCTAAGTGCGGCTGACCATCCGTATCATAAATTTAGTACAGGGAATTGGGAAACTTTGGAGGTCCGTCCAAAAGCGAAAGGATTGGATACCAGAGATGAGCTTATTAAGTTCTATGAACAATATTATTCTGCTAATGTCATGCATCTTGTCATTTATGGAAAAGAAAACCTTGATAAAATTCAAGCCACTGTAGAGGACAAGTTTAAGGATATTAGAAACATTGACCGCAACTGCCTTTGTTTTGGTGGTGAGCCTTGCACATCAGAACATTTGCAGATTCTTGTCAAAACTGTTCCAATCAAAGAGGGTCATAAGCTGAGACTTGTGTGGCCTATAGCTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTAGTCATCTCATTGGCCACGAAGCAGAGGGTTCTTTGTATGCCGTCTTGAAAACTTTGGGATGGGCAACAGGTTTGTCTGCTGGGGAATCGGATTCAACGTTGGACTTTTCTTTCTTTAGAGTAGATATGGATCTCACGGACGCAGGTCATGAGCACATGCAAGACATAGTAGGTTTGCTTTTCAAATACATTTCCCTCTTACAGCAGTCGGGTGTTTGCAAGTGGATTTTTGATGAGCTTTCTGCTGTTTGCAAGACGAAGTTTCATTATCAGGACAAAATGCAGCCTATTAATTATGTCGTCAGCATTTCGATAAATATGCAGAGATATCCCCCAAGAGATTGGCTAGTAAGATCATCCTTGCCGTCTAACTTCAGTCCAGACACTATCCAAATGGTGCTTAACAAGCTTTCTCCGGACAAAGTCAGAATTTTCTGGGAATCAAAAAAATTTGAAGGTCATACTAACATGACTGAGCCATGGTATGGAACTGCTTATTCCGTCGAGAAAATTAGTGGCTCAATGATTCAGGAATGGATAGTATCTTCCCCAAATGAGAATCTCCACTTACCAGCCCCTAATGTGTTCATCCCCACTGACTTGTCGCTTAAGAATAATCATGAGAAGGCCAATTGTCCAGTTCTGTTAAGAAAGTCGCCGTGTTCAACACTTTGGTACAAGCCCGATACAATGTTCTTTACTCCTAAGGCTTACGTTAAGATTGATTTCACTTGTCCCCACGCAAGTGAATCCCCTGAAACAGAAGTCCTAACTAATATGTTTACTCAGCTGTTGATGGATTACTTGAATGAAAATGCTTACTATGCTCAGGTTGCTGAACTGTTTTACAGAATAACCCACACAGATAGTGGGTTTCAGGTGACTCTGGTTGGCTATAACCACAAATTAAGAATTTTACTGGAAACTGTAGTTGAGAAGATTGCAAGCTTTAAAGTGAAAGCTGATAGGTTCGCAGTCATCAAGGAAATGCTCATAAAGGAGTACCAAAACTTGAAATTTCAACAGCCTTATGAGCAGGCTATGAACTACTGCGCATTAATTCTACAGGATCATACTTGTCCATGGACGGAAGAACTTGAAGCTCTTCCTCACCTTCAAGTTGAAGATCTTGCTAAGTTTGTCCCTTGGATGCTTTCGAGGGCCTTCGTAGAGTGTTATACAGCTGGAAACCTTGAAAAGAATGAAGCTGAGTCAATGATCCAGCATATTGAAGATGTTTTCTTTAAGGGCTCAAATCATATTTCCCAAACTTTATTTCCATCCCAGCATTTGACGAATAGAGTCGTGAAGCTTGAAAAGGGCAACAACTACTTCTACCCTGTGGAAGGACTTAATCCAAGTGACGAGAATTCTGCCCTTGTCCACTATATCCAGGTTCATCGGGATGACTTTATGCTGAATGTGAAACTTCAGCTGTTCGCTCTTATTGCAAAGCAGCCTGCCTTTCACCAGCTTAGATCTGTTGAGCAACTTGGTTACATCACGGCCCTCTTGCAGAGGAATGATTCTGGTATCCGTGGGGTGCAGTTTATTATACAATCCACTGCGAAGGATCCAGCACATATCGACTTGAGAGTGGAGGAATTCCTCAAGGGGTTCGAGAGTAAACTTTATGACATGCCTAGTGACGAATTCAAGAGCAACGTAAATGCATTGATCAACATGAAGCTTGAGAAGCACAAGAATTTAATGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCGGATGGGACACTCAAGTTTGATAGGAAAGAGTCCGAGATTGCAGCACTGAGGCAGCTTACACAGCAAGAATTGATAGATTTTTTCAATGAGCATATAAAAGTTGGGGCTCCTCAAAAGAGGTCGTTAAGCGTACGAGTGTACGGGAACTCACATTCATCGGAGTACGCTACAGATACAAGCTCACCGGTGCAACCTTGCTGCGTCAAAATAGACGATATATTCAGCTTCAGAAGGTCACAACCTCTTTATGGTTCATTCAAGGGAAATCATGTGAAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

967

Amino Acids

111.3

Weight (kDa)

5.76

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 32 - 167 1.6e-37 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 194 - 371 7.7e-31 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 379 - 659 4.2e-103 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 663 - 850 4.3e-10 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 770 - 869 1.5e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1927
Acc36I ACCTGC 1 cut(s) 1007
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccIII TCCGGA 1 cut(s) 1288
AciI CCGC 3 cut(s) 5, 500, 718
AclI AACGTT 1 cut(s) 971
AclWI GGATC 6 cut(s) 104, 1011, 1950, 2086, 2441, 2454
AcoI YGGCCR 1 cut(s) 886
AcuI CTGAAG 4 cut(s) 420, 1231, 2288, 2830
AfiI CCNNNNNNNGG 5 cut(s) 241, 850, 1358, 2219, 2867
AflII CTTAAG 1 cut(s) 1481
AgeI ACCGGT 1 cut(s) 2803
AjnI CCWGG 2 cut(s) 177, 2265
AjuI GAANNNNNNNTTGG 2 cut(s) 2133, 2165
AleI CACNNNNGTG 1 cut(s) 1617
Alw21I GWGCWC 2 cut(s) 309, 1029
Alw26I GTCTC 1 cut(s) 805
AlwI GGATC 6 cut(s) 104, 1011, 1950, 2086, 2441, 2454
AlwNI CAGNNNCTG 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 1288
AoxI GGCC 7 cut(s) 175, 821, 857, 886, 1500, 2034, 2379
ApeKI GCWGC 6 cut(s) 131, 1156, 2329, 2660, 2671, 2817
AseI ATTAAT 2 cut(s) 1164, 1931
AsiGI ACCGGT 1 cut(s) 2803
Asp700I GAANNNNTTC 2 cut(s) 96, 1977
AspLEI GCGC 1 cut(s) 1928
AspS9I GGNCC 4 cut(s) 553, 857, 2034, 2380
AsuHPI GGTGA 7 cut(s) 256, 752, 833, 1765, 1976, 2333, 2793
AvaII GGWCC 1 cut(s) 553
AxyI CCTNAGG 1 cut(s) 1578
BalI TGGCCA 1 cut(s) 888
BamHI GGATCC 1 cut(s) 2446
BanII GRGCYC 2 cut(s) 2126, 2728
Bbv12I GWGCWC 2 cut(s) 309, 1029
BbvI GCAGC 6 cut(s) 118, 1168, 2341, 2672, 2683, 2804
BccI CCATC 7 cut(s) 167, 332, 516, 927, 1663, 2161, 2615
BceAI ACGGC 4 cut(s) 899, 1222, 1516, 2394
BcgI CGANNNNNNTGC 4 cut(s) 1174, 1208, 2228, 2262
BciT130I CCWGG 2 cut(s) 179, 2267
BciVI GTATCC 2 cut(s) 571, 2417
BclI TGATCA 1 cut(s) 2550
BcoDI GTCTC 1 cut(s) 805
BfaI CTAG 4 cut(s) 77, 222, 1220, 2517
BfmI CTRYAG 5 cut(s) 681, 825, 1800, 1937, 2787
BfrI CTTAAG 1 cut(s) 1481
BfuAI ACCTGC 1 cut(s) 1007
BfuI GTATCC 2 cut(s) 571, 2417
BglII AGATCT 2 cut(s) 1999, 2351
BisI GCNGC 7 cut(s) 132, 501, 1157, 2330, 2661, 2672, 2818
BlsI GCNGC 7 cut(s) 133, 502, 1158, 2331, 2662, 2673, 2819
Bme1390I CCNGG 2 cut(s) 179, 2267
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 4 cut(s) 553, 857, 2034, 2380
BmiI GGNNCC 2 cut(s) 2448, 2727
BmrFI CCNGG 2 cut(s) 179, 2267
BmsI GCATC 3 cut(s) 451, 643, 2013
BoxI GACNNNNGTC 1 cut(s) 1017
BpmI CTGGAG 3 cut(s) 200, 467, 816
Bpu10I CCTNAGC 1 cut(s) 1701
BpuEI CTTGAG 5 cut(s) 221, 2468, 2485, 2585, 2615
BsaBI GATNNNNATC 1 cut(s) 1002
BsaJI CCNNGG 5 cut(s) 138, 1352, 1955, 2018, 2410
BsaWI WCCGGW 2 cut(s) 1288, 2803
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 850, 1358, 2219, 2867
Bse118I RCCGGY 1 cut(s) 2803
Bse1I ACTGG 2 cut(s) 1511, 1797
Bse21I CCTNAGG 1 cut(s) 1578
Bse8I GATNNNNATC 1 cut(s) 1002
BseAI TCCGGA 1 cut(s) 1288
BseBI CCWGG 2 cut(s) 179, 2267
BseDI CCNNGG 5 cut(s) 138, 1352, 1955, 2018, 2410
BseGI GGATG 9 cut(s) 148, 508, 938, 1229, 1461, 2028, 2153, 2284, 2626
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 5 cut(s) 241, 850, 1358, 2219, 2867
BseMII CTCAG 9 cut(s) 294, 383, 426, 800, 1338, 1673, 1715, 2073, 2657
BseNI ACTGG 2 cut(s) 1511, 1797
BseRI GAGGAG 1 cut(s) 2718
BseXI GCAGC 6 cut(s) 118, 1168, 2341, 2672, 2683, 2804
BseYI CCCAGC 2 cut(s) 953, 2157
BsgI GTGCAG 1 cut(s) 2438
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 7 cut(s) 177, 823, 859, 888, 1502, 2036, 2381
BshTI ACCGGT 1 cut(s) 2803
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 309, 1029
BsiSI CCGG 2 cut(s) 1289, 2804
BsiWI CGTACG 2 cut(s) 44, 2749
BslFI GGGAC 3 cut(s) 1593, 2000, 2638
BslI CCNNNNNNNGG 5 cut(s) 241, 850, 1358, 2219, 2867
BsmAI GTCTC 1 cut(s) 805
BsmFI GGGAC 3 cut(s) 1593, 2000, 2638
BsnI GGCC 7 cut(s) 177, 823, 859, 888, 1502, 2036, 2381
Bsp1286I GDGCHC 4 cut(s) 309, 1029, 2126, 2728
Bsp13I TCCGGA 1 cut(s) 1288
Bsp19I CCATGG 3 cut(s) 138, 1352, 1955
BspACI CCGC 3 cut(s) 5, 500, 718
BspANI GGCC 7 cut(s) 177, 823, 859, 888, 1502, 2036, 2381
BspCNI CTCAG 9 cut(s) 295, 382, 427, 801, 1339, 1672, 1714, 2074, 2658
BspEI TCCGGA 1 cut(s) 1288
BspHI TCATGA 2 cut(s) 1021, 1492
BspLI GGNNCC 2 cut(s) 2448, 2727
BspMI ACCTGC 1 cut(s) 1007
BspPI GGATC 6 cut(s) 104, 1011, 1950, 2086, 2441, 2454
BspQI GCTCTTC 1 cut(s) 1983
BspTI CTTAAG 1 cut(s) 1481
BsrFI RCCGGY 1 cut(s) 2803
BsrI ACTGG 2 cut(s) 1511, 1797
BssAI RCCGGY 1 cut(s) 2803
BssECI CCNNGG 5 cut(s) 138, 1352, 1955, 2018, 2410
BssT1I CCWWGG 4 cut(s) 138, 1352, 1955, 2018
Bst2UI CCWGG 2 cut(s) 179, 2267
Bst4CI ACNGT 4 cut(s) 682, 784, 1716, 1801
Bst6I CTCTTC 3 cut(s) 12, 342, 1983
BstAFI CTTAAG 1 cut(s) 1481
BstC8I GCNNGC 3 cut(s) 1819, 1911, 2334
BstDSI CCRYGG 4 cut(s) 138, 1352, 1955, 2410
BstENI CCTNNNNNAGG 1 cut(s) 2217
BstF5I GGATG 9 cut(s) 148, 508, 938, 1229, 1461, 2028, 2153, 2284, 2626
BstHHI GCGC 1 cut(s) 1928
BstMAI GTCTC 1 cut(s) 805
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 8 cut(s) 277, 1279, 1697, 1706, 1906, 2321, 2569, 2680
BstNI CCWGG 2 cut(s) 179, 2267
BstNSI RCATGY 2 cut(s) 1033, 2515
BstPAI GACNNNNGTC 1 cut(s) 1017
BstSCI CCNGG 2 cut(s) 177, 2265
BstSFI CTRYAG 5 cut(s) 681, 825, 1800, 1937, 2787
BstV1I GCAGC 6 cut(s) 118, 1168, 2341, 2672, 2683, 2804
BstX2I RGATCY 4 cut(s) 1003, 1999, 2351, 2446
BstXI CCANNNNNNTGG 1 cut(s) 1215
BstYI RGATCY 4 cut(s) 1003, 1999, 2351, 2446
Bsu36I CCTNAGG 1 cut(s) 1578
BsuI GTATCC 2 cut(s) 571, 2417
BsuRI GGCC 7 cut(s) 177, 823, 859, 888, 1502, 2036, 2381
BtgI CCRYGG 4 cut(s) 138, 1352, 1955, 2410
BtsCI GGATG 9 cut(s) 148, 508, 938, 1229, 1461, 2028, 2153, 2284, 2626
BtsI GCAGTG 1 cut(s) 2436
BtsIMutI CAGTG 4 cut(s) 678, 1467, 2436, 2663
BveI ACCTGC 1 cut(s) 1007
Cac8I GCNNGC 3 cut(s) 1819, 1911, 2334
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 2 cut(s) 1021, 1492
CfoI GCGC 1 cut(s) 1928
Cfr10I RCCGGY 1 cut(s) 2803
Cfr13I GGNCC 4 cut(s) 553, 857, 2034, 2380
CseI GACGC 2 cut(s) 1022, 2809
CspAI ACCGGT 1 cut(s) 2803
DraI TTTAAA 1 cut(s) 1825
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 3 cut(s) 12, 342, 1983
EarI CTCTTC 3 cut(s) 12, 342, 1983
Eco130I CCWWGG 4 cut(s) 138, 1352, 1955, 2018
Eco24I GRGCYC 2 cut(s) 2126, 2728
Eco32I GATATC 1 cut(s) 1202
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 4 cut(s) 420, 1231, 2288, 2830
Eco81I CCTNAGG 1 cut(s) 1578
EcoNI CCTNNNNNAGG 1 cut(s) 2217
EcoO109I RGGNCCY 1 cut(s) 2034
EcoRI GAATTC 3 cut(s) 2242, 2477, 2525
EcoRII CCWGG 2 cut(s) 177, 2265
EcoRV GATATC 1 cut(s) 1202
EcoT14I CCWWGG 4 cut(s) 138, 1352, 1955, 2018
EcoT22I ATGCAT 3 cut(s) 466, 636, 2548
EcoT38I GRGCYC 2 cut(s) 2126, 2728
ErhI CCWWGG 4 cut(s) 138, 1352, 1955, 2018
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FaqI GGGAC 3 cut(s) 1593, 2000, 2638
FauNDI CATATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 2550
Fnu4HI GCNGC 7 cut(s) 132, 501, 1157, 2330, 2661, 2672, 2818
FokI GGATG 9 cut(s) 155, 495, 945, 1216, 1448, 2035, 2140, 2291, 2633
FriOI GRGCYC 2 cut(s) 2126, 2728
Fsp4HI GCNGC 7 cut(s) 132, 501, 1157, 2330, 2661, 2672, 2818
FspBI CTAG 4 cut(s) 77, 222, 1220, 2517
FspI TGCGCA 1 cut(s) 1927
GlaI GCGC 1 cut(s) 1927
GluI GCNGC 7 cut(s) 132, 501, 1157, 2330, 2661, 2672, 2818
GsaI CCCAGC 2 cut(s) 957, 2161
GsuI CTGGAG 3 cut(s) 200, 467, 816
HaeIII GGCC 7 cut(s) 177, 823, 859, 888, 1502, 2036, 2381
HapII CCGG 2 cut(s) 1289, 2804
HgaI GACGC 2 cut(s) 1022, 2809
HhaI GCGC 1 cut(s) 1928
Hin6I GCGC 1 cut(s) 1926
HinP1I GCGC 1 cut(s) 1926
HindIII AAGCTT 4 cut(s) 1280, 1819, 2183, 2561
HpaII CCGG 2 cut(s) 1289, 2804
HphI GGTGA 7 cut(s) 256, 752, 833, 1765, 1976, 2333, 2793
Hpy166II GTNNAC 4 cut(s) 1656, 2257, 2501, 2758
Hpy8I GTNNAC 4 cut(s) 1656, 2257, 2501, 2758
Hpy99I CGWCG 1 cut(s) 1379
HpyCH4III ACNGT 4 cut(s) 682, 784, 1716, 1801
HpyCH4IV ACGT 3 cut(s) 971, 1587, 2538
HpyF10VI GCNNNNNNNGC 8 cut(s) 277, 1279, 1697, 1706, 1906, 2321, 2569, 2680
HpySE526I ACGT 3 cut(s) 971, 1587, 2538
HspAI GCGC 1 cut(s) 1926
Kpn2I TCCGGA 1 cut(s) 1288
Ksp22I TGATCA 1 cut(s) 2550
LguI GCTCTTC 1 cut(s) 1983
LmnI GCTCC 3 cut(s) 486, 835, 2731
Lsp1109I GCAGC 6 cut(s) 118, 1168, 2341, 2672, 2683, 2804
LweI GCATC 3 cut(s) 451, 643, 2013
MaeI CTAG 4 cut(s) 77, 222, 1220, 2517
MaeII ACGT 3 cut(s) 971, 1587, 2538
MaeIII GTNAC 6 cut(s) 248, 1753, 2234, 2369, 2519, 2853
MboII GAAGA 9 cut(s) 29, 359, 1410, 1822, 1970, 1976, 2009, 2117, 2600
MfeI CAATTG 1 cut(s) 1504
MflI RGATCY 4 cut(s) 1003, 1999, 2351, 2446
MhlI GDGCHC 4 cut(s) 309, 1029, 2126, 2728
MlsI TGGCCA 1 cut(s) 888
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 5 cut(s) 293, 1750, 2093, 2184, 2657
MmeI TCCRAC 1 cut(s) 954
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 3 cut(s) 466, 636, 2548
MroI TCCGGA 1 cut(s) 1288
MroXI GAANNNNTTC 2 cut(s) 96, 1977
MscI TGGCCA 1 cut(s) 888
MslI CAYNNNNRTG 3 cut(s) 495, 1617, 2277
Msp20I TGGCCA 1 cut(s) 888
MspA1I CMGCKG 4 cut(s) 398, 1663, 2057, 2308
MspCI CTTAAG 1 cut(s) 1481
MspI CCGG 2 cut(s) 1289, 2804
MspR9I CCNGG 2 cut(s) 179, 2267
MunI CAATTG 1 cut(s) 1504
MvaI CCWGG 2 cut(s) 179, 2267
MwoI GCNNNNNNNGC 8 cut(s) 277, 1279, 1697, 1706, 1906, 2321, 2569, 2680
NcoI CCATGG 3 cut(s) 138, 1352, 1955
NdeI CATATG 1 cut(s) 207
NlaIV GGNNCC 2 cut(s) 2448, 2727
NmuCI GTSAC 4 cut(s) 1753, 2234, 2519, 2853
NsbI TGCGCA 1 cut(s) 1927
NsiI ATGCAT 3 cut(s) 466, 636, 2548
NspI RCATGY 2 cut(s) 1033, 2515
OliI CACNNNNGTG 1 cut(s) 1617
PagI TCATGA 2 cut(s) 1021, 1492
PciSI GCTCTTC 1 cut(s) 1983
PcsI WCGNNNNNNNCGW 1 cut(s) 968
PdmI GAANNNNTTC 2 cut(s) 96, 1977
Pfl23II CGTACG 2 cut(s) 44, 2749
PflFI GACNNNGTC 1 cut(s) 1295
PflMI CCANNNNNTGG 1 cut(s) 1358
PinAI ACCGGT 1 cut(s) 2803
PkrI GCNGC 7 cut(s) 133, 502, 1158, 2331, 2662, 2673, 2819
PleI GAGTC 5 cut(s) 293, 1750, 2092, 2183, 2656
PpsI GAGTC 5 cut(s) 293, 1750, 2092, 2183, 2656
PshAI GACNNNNGTC 1 cut(s) 1017
PshBI ATTAAT 2 cut(s) 1164, 1931
Psp1406I AACGTT 1 cut(s) 971
Psp6I CCWGG 2 cut(s) 177, 2265
PspFI CCCAGC 2 cut(s) 953, 2157
PspGI CCWGG 2 cut(s) 177, 2265
PspLI CGTACG 2 cut(s) 44, 2749
PspN4I GGNNCC 2 cut(s) 2448, 2727
PspPI GGNCC 4 cut(s) 553, 857, 2034, 2380
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 4 cut(s) 1003, 1999, 2351, 2446
PsyI GACNNNGTC 1 cut(s) 1295
PvuII CAGCTG 4 cut(s) 398, 1663, 2057, 2308
RseI CAYNNNNRTG 3 cut(s) 495, 1617, 2277
SapI GCTCTTC 1 cut(s) 1983
SatI GCNGC 7 cut(s) 132, 501, 1157, 2330, 2661, 2672, 2818
Sau96I GGNCC 4 cut(s) 553, 857, 2034, 2380
SchI GAGTC 5 cut(s) 293, 1750, 2093, 2184, 2657
ScrFI CCNGG 2 cut(s) 179, 2267
SduI GDGCHC 4 cut(s) 309, 1029, 2126, 2728
SfaNI GCATC 3 cut(s) 451, 643, 2013
SfcI CTRYAG 5 cut(s) 681, 825, 1800, 1937, 2787
SgrAI CRCCGGYG 1 cut(s) 2803
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 3 cut(s) 495, 1617, 2277
SmlI CTYRAG 6 cut(s) 200, 1481, 2464, 2483, 2564, 2630
SmoI CTYRAG 6 cut(s) 200, 1481, 2464, 2483, 2564, 2630
SsiI CCGC 3 cut(s) 5, 500, 718
SspI AATATT 1 cut(s) 614
SspMI CTAG 4 cut(s) 77, 222, 1220, 2517
StyD4I CCNGG 2 cut(s) 177, 2265
StyI CCWWGG 4 cut(s) 138, 1352, 1955, 2018
TaaI ACNGT 4 cut(s) 682, 784, 1716, 1801
TaiI ACGT 3 cut(s) 974, 1590, 2541
TaqI TCGA 7 cut(s) 90, 254, 1184, 1377, 2030, 2460, 2493
TatI WGTACW 3 cut(s) 60, 258, 527
TauI GCSGC 1 cut(s) 503
TscAI CASTG 4 cut(s) 685, 1474, 2443, 2670
TseFI GTSAC 4 cut(s) 1753, 2234, 2519, 2853
TseI GCWGC 6 cut(s) 131, 1156, 2329, 2660, 2671, 2817
Tsp45I GTSAC 4 cut(s) 1753, 2234, 2519, 2853
TspGWI ACGGA 7 cut(s) 62, 501, 545, 1025, 1363, 1976, 2399
TspRI CASTG 4 cut(s) 685, 1474, 2443, 2670
Tth111I GACNNNGTC 1 cut(s) 1295
Van91I CCANNNNNTGG 1 cut(s) 1358
Vha464I CTTAAG 1 cut(s) 1481
VpaK11BI GGWCC 1 cut(s) 553
VspI ATTAAT 2 cut(s) 1164, 1931
XagI CCTNNNNNAGG 1 cut(s) 2217
XceI RCATGY 2 cut(s) 1033, 2515
XcmI CCANNNNNNNNNTGG 1 cut(s) 1739
XmnI GAANNNNTTC 2 cut(s) 96, 1977
XspI CTAG 4 cut(s) 77, 222, 1220, 2517
Zsp2I ATGCAT 3 cut(s) 466, 636, 2548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.