AT3G57470

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001584
Physical Location & Seq
Reverse (-)
21269149 .. 21276017
6869 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57470.4

Sequence Viewer

Length: 2556 bp
ATGGCGGTTGGAATGGAGAACGCGACGGCGTCTGGTGAATGTGGCGAGATACTTAAGCCACGCACCGACAAGAGAGAGTATCGGAGGATTGTTCTGAAGAATTCGCTGGAGGTGTTGTTAATCAGCGACCCAGAGACTGACAAGTGTGCTGCTTCAATGAACGTTAGCGTTGGATCGTTTACGGACCCAGAAGGATTGGAAGGGCTAGCTCATTTCCTTGAGCATATGCTGTTTTATGCAAGTGAAAAATACCCTGAGGAAGATAGTTACTCCAAGTACATCACAGAGCATGGAGGGAGCACAAATGCTTATACTTCCAGTGAAGATACAAACTACCATTTCGATATCAACACTGATTCTTTTTATGAAGCTTTGGACAGGTTTGCACAGTTCTTTATCCAGCCACTGATGTCAACTGATGCCACCATGAGGGAGATCAAGGCGGTCGACTCTGAGCATCAGAATAACTTGTTGTCTGACTCTTGGCGTATGGCCCAGTTACAGAAGCATCTAAGTAGAGAAGACCATCCATATCACAAATTTAGCACAGGGAATATGGATACTCTTCACGTACGACCCGAAGAAAATGGAGTGGATACAAGGAGTGAACTAATTAAATTTTATGACGAACACTATTCTGCCAACATCATGCATCTGGTTGTTTATGGCAAGGAAAATCTTGATAAAACTCAAGGTCTAGTGGAAGCGTTGTTCCAGGGAATTCGAAACACCAACCAGGGTATTCCAAGATTTCCTGGTCAGCCGTGTACTCTAGACCATTTGCAGGTTCTCGTGAAGGCTGTTCCTATAATGCAGGGTCACGAGCTTAGTGTTTCATGGCCTGTAACTCCTAGCATCTCTCATTATGAAGAGGCACCATGCAGGTATCTTGGTGATCTAATTGGTCATGAAGGCGAAGGAAGTCTGTTTCATGCCTTAAAAATCTTGGGTTGGGCAACAGGACTCTATGCTGGTGAAGCAGACTGGAGTATGGAATATTCTTTCTTCAATGTTTCAATTGATCTTACTGATGCTGGTCATGAGCATATGCAAGATATTTTAGGGTTGTTGTTCGAATACATTAAGGTTTTACAACAGTCTGGTGTTTCCCAGTGGATTTTTGATGAGCTCTCAGCTATATGTGAGGCAGAATTTCATTATCAAGCCAAAATAGATCCAATTTCTTATGCAGTGGATATTTCTTCAAACATGAAGATATATCCAACCAAGCATTGGCTGGTAGGATCATCACTGCCTTCGAAATTTAATCCAGCTATTGTGCAAAAGGTTCTCGATGAGCTTTCTCCCAATAATGTTCGAATCTTTTGGGAGTCAAATAAATTTGAAGGGCAAACTGACAAGGTTGAGCCATGGTATAACACTGCTTATTCACTTGAGAAGATAACCAAATTCACCATTCAGGAGTGGATGCAGTCAGCCCCTGATGTAAACCTGCTTCTACCAACACCTAATGTCTTCATTCCTACGGATTTTTCATTAAAGGATTTAAAGGATAAGGATATCTTTCCTGTTTTGCTGAGAAAGACATCATACTCAAGATTGTGGTATAAGCCTGACACAAAGTTCTTCAAACCAAAGGCATACGTTAAGATGGATTTTAACTGCCCCCTTGCAGTCAGCTCTCCCGACGCTGCGGTTCTTTCAGATATTTTTGTTTGGTTGCTAGTGGACTATTTGAATGAATATGCTTATTATGCTCAGGCTGCTGGTCTTGACTATGGATTAAGTCTTTCGGACAATGGCTTTGAGCTCTCTCTTGCTGGCTTTAATCACAAATTGAGGATCTTGCTGGAAGCTGTCATTCAAAAGATAGCAAAATTCGAAGTTAAACCTGACAGGTTTTCCGTTATCAAGGAAACAGTCACAAAGGCATACCAAAATAACAAATTCCAACAACCACATGAACAAGCAACGAATTACTGCTCACTAGTATTACAAGATCAGATTTGGCCTTGGACAGAGGAACTAGATGCTCTTTCTCATTTGGAAGCTGAAGACTTGGCTAATTTCGTTCCGATGCTATTATCAAGGACATTTGTTGAGTGCTATATAGCAGGGAATGTTGAGAAGGACGAAGCTGAGTCAATGGTCAAGCATATTGAAGATGTTCTTTTTACCGACTCAAAACCTATTTGTCGTCCGTTATTTCCATCCCAGTTCCTGACAAATAGGGTTACAGAGCTCGGAACAGGAATGAAACACTTTTACTATCAGGAAGGTTCAAACTCCAGTGATGAAAATTCTGCCCTAGTGCATTATATTCAGGTTCATAAAGATGAGTTTTCCATGAATAGTAAACTTCAGCTTTTCGAACTAATTGCAAAACAAGACACGTTTCACCAGCTTAGAACGATTGAGCAACTTGGCTACATCACCTCACTTTCTCTGAGCAACGATTCTGGCGTCTATGGTGTACAATTCATTATCCAGTCTTCAGTTAAGGGTCCAGGACATATTGATTCGAGAGTTGAGTCACTACTAAAGGACCTGGAGAGTAAATTTTACAATATGAGCGATGAAGAATTCAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

851

Amino Acids

97.18

Weight (kDa)

4.99

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 1 - 97 7.8e-21 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 125 - 302 2.1e-33 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 310 - 590 1.6e-100 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 594 - 779 1.6e-16 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 703 - 800 6e-19 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 775, 873, 1461
AccB1I GGYRCC 1 cut(s) 874
AccB7I CCANNNNNTGG 1 cut(s) 1233
AccI GTMKAC 1 cut(s) 447
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 5, 443, 1655
AclI AACGTT 1 cut(s) 162
AclWI GGATC 4 cut(s) 181, 1169, 1252, 1811
AcuI CTGAAG 4 cut(s) 116, 2034, 2306, 2439
AcyI GRCGYC 2 cut(s) 29, 2424
AfaI GTAC 4 cut(s) 278, 573, 769, 2436
AfiI CCNNNNNNNGG 3 cut(s) 429, 784, 1233
AflII CTTAAG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 2352
AhlI ACTAGT 1 cut(s) 1948
AjnI CCWGG 5 cut(s) 714, 735, 754, 2467, 2508
AloI GAACNNNNNNTCC 2 cut(s) 695, 727
Alw21I GWGCWC 4 cut(s) 302, 1131, 1773, 2205
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 4 cut(s) 181, 1169, 1252, 1811
AlwNI CAGNNNCTG 4 cut(s) 137, 406, 1442, 2182
AoxI GGCC 3 cut(s) 492, 839, 1970
ApeKI GCWGC 3 cut(s) 149, 1652, 1724
ArsI GACNNNNNNTTYG 2 cut(s) 1748, 1780
Asp700I GAANNNNTTC 1 cut(s) 2127
AspS9I GGNCC 4 cut(s) 184, 493, 2465, 2506
AsuHPI GGTGA 6 cut(s) 47, 905, 986, 1405, 2351, 2386
AsuII TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
AsuNHI GCTAGC 1 cut(s) 205
AvaII GGWCC 3 cut(s) 184, 2465, 2506
AxyI CCTNAGG 1 cut(s) 255
BanI GGYRCC 1 cut(s) 874
BanII GRGCYC 3 cut(s) 1131, 1773, 2205
BarI GAAGNNNNNNTAC 2 cut(s) 1440, 1472
BauI CACGAG 2 cut(s) 791, 821
BbsI GAAGAC 4 cut(s) 528, 1468, 2022, 2445
Bbv12I GWGCWC 4 cut(s) 302, 1131, 1773, 2205
BbvI GCAGC 3 cut(s) 136, 1639, 1711
BccI CCATC 3 cut(s) 534, 1606, 2179
BceAI ACGGC 2 cut(s) 42, 748
BcgI CGANNNNNNTGC 2 cut(s) 2321, 2355
BciT130I CCWGG 5 cut(s) 716, 737, 756, 2469, 2510
BciVI GTATCC 2 cut(s) 553, 589
BcoDI GTCTC 1 cut(s) 128
BcuI ACTAGT 1 cut(s) 1948
BfaI CTAG 8 cut(s) 206, 698, 773, 852, 1685, 1949, 1988, 2270
BfrI CTTAAG 1 cut(s) 53
BfuAI ACCTGC 3 cut(s) 775, 873, 1461
BfuI GTATCC 2 cut(s) 553, 589
BisI GCNGC 3 cut(s) 150, 1653, 1725
BlsI GCNGC 3 cut(s) 151, 1654, 1726
Bme1390I CCNGG 5 cut(s) 716, 737, 756, 2469, 2510
Bme18I GGWCC 3 cut(s) 184, 2465, 2506
BmgT120I GGNCC 4 cut(s) 184, 493, 2465, 2506
BmiI GGNNCC 3 cut(s) 186, 876, 2466
BmrFI CCNGG 5 cut(s) 716, 737, 756, 2469, 2510
BmrI ACTGGG 3 cut(s) 490, 1105, 2170
BmsI GCATC 9 cut(s) 409, 466, 517, 661, 864, 1021, 1419, 1981, 2028
BmtI GCTAGC 1 cut(s) 209
BmuI ACTGGG 3 cut(s) 490, 1105, 2170
BpiI GAAGAC 4 cut(s) 528, 1468, 2022, 2445
BpmI CTGGAG 4 cut(s) 128, 1006, 2233, 2531
Bpu10I CCTNAGC 1 cut(s) 1719
Bpu14I TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
BpuEI CTTGAG 4 cut(s) 239, 675, 1415, 1540
BsaAI YACGTR 1 cut(s) 571
BsaHI GRCGYC 2 cut(s) 29, 2424
BsaJI CCNNGG 4 cut(s) 715, 736, 1370, 1973
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 784, 1233
Bse1I ACTGG 7 cut(s) 318, 496, 989, 1111, 2176, 2250, 2449
Bse21I CCTNAGG 1 cut(s) 255
BseBI CCWGG 5 cut(s) 716, 737, 756, 2469, 2510
BseDI CCNNGG 4 cut(s) 715, 736, 1370, 1973
BseGI GGATG 3 cut(s) 526, 1434, 2171
BseLI CCNNNNNNNGG 3 cut(s) 429, 784, 1233
BseMII CTCAG 7 cut(s) 246, 444, 1146, 1529, 1733, 2091, 2399
BseNI ACTGG 7 cut(s) 318, 496, 989, 1111, 2176, 2250, 2449
BseXI GCAGC 3 cut(s) 136, 1639, 1711
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 447
BshFI GGCC 3 cut(s) 494, 841, 1972
BshNI GGYRCC 1 cut(s) 874
BsiEI CGRYCG 1 cut(s) 447
BsiHKAI GWGCWC 4 cut(s) 302, 1131, 1773, 2205
BsiWI CGTACG 1 cut(s) 571
BslI CCNNNNNNNGG 3 cut(s) 429, 784, 1233
BsmAI GTCTC 1 cut(s) 128
BsnI GGCC 3 cut(s) 494, 841, 1972
Bsp119I TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
Bsp1286I GDGCHC 4 cut(s) 302, 1131, 1773, 2205
Bsp1407I TGTACA 1 cut(s) 2434
Bsp143I GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
Bsp19I CCATGG 1 cut(s) 1370
BspACI CCGC 3 cut(s) 5, 443, 1655
BspANI GGCC 3 cut(s) 494, 841, 1972
BspCNI CTCAG 7 cut(s) 247, 445, 1145, 1530, 1732, 2092, 2400
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 2 cut(s) 907, 1039
BspLI GGNNCC 3 cut(s) 186, 876, 2466
BspMI ACCTGC 3 cut(s) 775, 873, 1461
BspOI GCTAGC 1 cut(s) 209
BspPI GGATC 4 cut(s) 181, 1169, 1252, 1811
BspT104I TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
BspT107I GGYRCC 1 cut(s) 874
BspTI CTTAAG 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 2434
BsrI ACTGG 7 cut(s) 318, 496, 989, 1111, 2176, 2250, 2449
BssECI CCNNGG 4 cut(s) 715, 736, 1370, 1973
BssMI GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
BssNI GRCGYC 2 cut(s) 29, 2424
BssSI CACGAG 2 cut(s) 791, 821
BssT1I CCWWGG 2 cut(s) 1370, 1973
Bst2BI CACGAG 2 cut(s) 791, 821
Bst2UI CCWGG 5 cut(s) 716, 737, 756, 2469, 2510
Bst4CI ACNGT 3 cut(s) 390, 1098, 1882
Bst6I CTCTTC 2 cut(s) 570, 864
BstACI GRCGYC 2 cut(s) 29, 2424
BstAFI CTTAAG 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 2434
BstBAI YACGTR 1 cut(s) 571
BstBI TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
BstC8I GCNNGC 2 cut(s) 207, 1783
BstDSI CCRYGG 1 cut(s) 1370
BstF5I GGATG 3 cut(s) 526, 1434, 2171
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 8 cut(s) 176, 438, 898, 1024, 1177, 1247, 1806, 1963
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
BstMCI CGRYCG 1 cut(s) 447
BstMWI GCNNNNNNNGC 3 cut(s) 977, 1715, 1724
BstNI CCWGG 5 cut(s) 716, 737, 756, 2469, 2510
BstSCI CCNGG 5 cut(s) 714, 735, 754, 2467, 2508
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 136, 1639, 1711
BstV2I GAAGAC 4 cut(s) 528, 1468, 2022, 2445
BstX2I RGATCY 2 cut(s) 1174, 1803
BstYI RGATCY 2 cut(s) 1174, 1803
Bsu36I CCTNAGG 1 cut(s) 255
BsuI GTATCC 2 cut(s) 553, 589
BsuRI GGCC 3 cut(s) 494, 841, 1972
BtgI CCRYGG 1 cut(s) 1370
BtgZI GCGATG 1 cut(s) 2550
BtsCI GGATG 3 cut(s) 526, 1434, 2171
BtsI GCAGTG 3 cut(s) 1197, 1250, 1380
BtsIMutI CAGTG 8 cut(s) 325, 351, 404, 1118, 1197, 1250, 1380, 2257
BveI ACCTGC 3 cut(s) 775, 873, 1461
Cac8I GCNNGC 2 cut(s) 207, 1783
CaiI CAGNNNCTG 4 cut(s) 137, 406, 1442, 2182
CciI TCATGA 2 cut(s) 907, 1039
Cfr13I GGNCC 4 cut(s) 184, 493, 2465, 2506
CseI GACGC 3 cut(s) 18, 1658, 2413
Csp6I GTAC 4 cut(s) 277, 572, 768, 2435
CviQI GTAC 4 cut(s) 277, 572, 768, 2435
DpnI GATC 8 cut(s) 175, 437, 897, 1023, 1176, 1246, 1805, 1962
DpnII GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
DraI TTTAAA 1 cut(s) 1509
Eam1104I CTCTTC 2 cut(s) 570, 864
EarI CTCTTC 2 cut(s) 570, 864
Ecl136II GAGCTC 3 cut(s) 1129, 1771, 2203
Eco130I CCWWGG 2 cut(s) 1370, 1973
Eco24I GRGCYC 3 cut(s) 1131, 1773, 2205
Eco32I GATATC 2 cut(s) 346, 1522
Eco47I GGWCC 3 cut(s) 184, 2465, 2506
Eco53kI GAGCTC 3 cut(s) 1129, 1771, 2203
Eco57I CTGAAG 4 cut(s) 116, 2034, 2306, 2439
Eco81I CCTNAGG 1 cut(s) 255
EcoICRI GAGCTC 3 cut(s) 1129, 1771, 2203
EcoO109I RGGNCCY 1 cut(s) 2506
EcoRI GAATTC 3 cut(s) 100, 720, 2543
EcoRII CCWGG 5 cut(s) 714, 735, 754, 2467, 2508
EcoRV GATATC 2 cut(s) 346, 1522
EcoT14I CCWWGG 2 cut(s) 1370, 1973
EcoT22I ATGCAT 1 cut(s) 654
EcoT38I GRGCYC 3 cut(s) 1131, 1773, 2205
ErhI CCWWGG 2 cut(s) 1370, 1973
FalI AAGNNNNNCTT 4 cut(s) 1508, 1540, 2115, 2147
FauNDI CATATG 2 cut(s) 225, 1047
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 3 cut(s) 150, 1653, 1725
FokI GGATG 3 cut(s) 513, 1441, 2158
FriOI GRGCYC 3 cut(s) 1131, 1773, 2205
Fsp4HI GCNGC 3 cut(s) 150, 1653, 1725
FspBI CTAG 8 cut(s) 206, 698, 773, 852, 1685, 1949, 1988, 2270
GluI GCNGC 3 cut(s) 150, 1653, 1725
GsuI CTGGAG 4 cut(s) 128, 1006, 2233, 2531
HaeIII GGCC 3 cut(s) 494, 841, 1972
HgaI GACGC 3 cut(s) 18, 1658, 2413
Hin1I GRCGYC 2 cut(s) 29, 2424
HincII GTYRAC 2 cut(s) 414, 448
HindII GTYRAC 2 cut(s) 414, 448
HindIII AAGCTT 1 cut(s) 369
HphI GGTGA 6 cut(s) 47, 905, 986, 1405, 2351, 2386
Hpy166II GTNNAC 9 cut(s) 180, 414, 448, 608, 768, 1450, 1690, 2318, 2435
Hpy8I GTNNAC 9 cut(s) 180, 414, 448, 608, 768, 1450, 1690, 2318, 2435
Hpy99I CGWCG 2 cut(s) 28, 1652
HpyAV CCTTC 9 cut(s) 185, 194, 790, 905, 911, 1266, 1340, 2083, 2231
HpyCH4III ACNGT 3 cut(s) 390, 1098, 1882
HpyCH4IV ACGT 4 cut(s) 162, 570, 1605, 2354
HpyF10VI GCNNNNNNNGC 3 cut(s) 977, 1715, 1724
HpySE526I ACGT 4 cut(s) 162, 570, 1605, 2354
Hsp92I GRCGYC 2 cut(s) 29, 2424
Kzo9I GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
LmnI GCTCC 1 cut(s) 297
Lsp1109I GCAGC 3 cut(s) 136, 1639, 1711
LweI GCATC 9 cut(s) 409, 466, 517, 661, 864, 1021, 1419, 1981, 2028
MaeI CTAG 8 cut(s) 206, 698, 773, 852, 1685, 1949, 1988, 2270
MaeII ACGT 4 cut(s) 162, 570, 1605, 2354
MaeIII GTNAC 7 cut(s) 266, 498, 818, 844, 1882, 2194, 2493
MalI GATC 8 cut(s) 175, 437, 897, 1023, 1176, 1246, 1805, 1962
MboI GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 2 cut(s) 1174, 1803
MhlI GDGCHC 4 cut(s) 302, 1131, 1773, 2205
MlyI GAGTC 7 cut(s) 443, 473, 957, 1340, 2111, 2135, 2501
MmeI TCCRAC 3 cut(s) 151, 1247, 1936
Mph1103I ATGCAT 1 cut(s) 654
MroXI GAANNNNTTC 1 cut(s) 2127
MslI CAYNNNNRTG 1 cut(s) 2295
MspCI CTTAAG 1 cut(s) 53
MspR9I CCNGG 5 cut(s) 716, 737, 756, 2469, 2510
MunI CAATTG 1 cut(s) 1017
MvaI CCWGG 5 cut(s) 716, 737, 756, 2469, 2510
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 977, 1715, 1724
NcoI CCATGG 1 cut(s) 1370
NdeI CATATG 2 cut(s) 225, 1047
NdeII GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
NheI GCTAGC 1 cut(s) 205
NlaIV GGNNCC 3 cut(s) 186, 876, 2466
NmuCI GTSAC 3 cut(s) 818, 1882, 2493
NsiI ATGCAT 1 cut(s) 654
NspV TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
PagI TCATGA 2 cut(s) 907, 1039
PcsI WCGNNNNNNNCGW 2 cut(s) 576, 2421
PdmI GAANNNNTTC 1 cut(s) 2127
PfeI GAWTC 4 cut(s) 356, 1320, 2417, 2480
Pfl23II CGTACG 1 cut(s) 571
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 1233
PfoI TCCNGGA 1 cut(s) 2467
PkrI GCNGC 3 cut(s) 151, 1654, 1726
PleI GAGTC 7 cut(s) 443, 473, 957, 1339, 2110, 2135, 2500
PpsI GAGTC 7 cut(s) 443, 473, 957, 1339, 2110, 2135, 2500
Ppu21I YACGTR 1 cut(s) 571
PpuMI RGGWCCY 1 cut(s) 2506
Psp124BI GAGCTC 3 cut(s) 1131, 1773, 2205
Psp1406I AACGTT 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 2506
Psp6I CCWGG 5 cut(s) 714, 735, 754, 2467, 2508
PspGI CCWGG 5 cut(s) 714, 735, 754, 2467, 2508
PspLI CGTACG 1 cut(s) 571
PspN4I GGNNCC 3 cut(s) 186, 876, 2466
PspPI GGNCC 4 cut(s) 184, 493, 2465, 2506
PspPPI RGGWCCY 1 cut(s) 2506
PstNI CAGNNNCTG 4 cut(s) 137, 406, 1442, 2182
PsuI RGATCY 2 cut(s) 1174, 1803
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 4 cut(s) 278, 573, 769, 2436
RsaNI GTAC 4 cut(s) 277, 572, 768, 2435
RseI CAYNNNNRTG 1 cut(s) 2295
SacI GAGCTC 3 cut(s) 1131, 1773, 2205
SalI GTCGAC 1 cut(s) 446
SatI GCNGC 3 cut(s) 150, 1653, 1725
Sau3AI GATC 8 cut(s) 173, 435, 895, 1021, 1174, 1244, 1803, 1960
Sau96I GGNCC 4 cut(s) 184, 493, 2465, 2506
SchI GAGTC 7 cut(s) 443, 473, 957, 1340, 2111, 2135, 2501
ScrFI CCNGG 5 cut(s) 716, 737, 756, 2469, 2510
SduI GDGCHC 4 cut(s) 302, 1131, 1773, 2205
SfaNI GCATC 9 cut(s) 409, 466, 517, 661, 864, 1021, 1419, 1981, 2028
SfuI TTCGAA 6 cut(s) 724, 1074, 1259, 1318, 1842, 2331
SinI GGWCC 3 cut(s) 184, 2465, 2506
SmiMI CAYNNNNRTG 1 cut(s) 2295
SmlI CTYRAG 5 cut(s) 53, 218, 690, 1394, 1555
SmoI CTYRAG 5 cut(s) 53, 218, 690, 1394, 1555
SpeI ACTAGT 1 cut(s) 1948
SsiI CCGC 3 cut(s) 5, 443, 1655
SspI AATATT 1 cut(s) 998
SspMI CTAG 8 cut(s) 206, 698, 773, 852, 1685, 1949, 1988, 2270
SstI GAGCTC 3 cut(s) 1131, 1773, 2205
StyD4I CCNGG 5 cut(s) 714, 735, 754, 2467, 2508
StyI CCWWGG 2 cut(s) 1370, 1973
TaaI ACNGT 3 cut(s) 390, 1098, 1882
TaiI ACGT 4 cut(s) 165, 573, 1608, 2357
TatI WGTACW 3 cut(s) 276, 767, 2434
TfiI GAWTC 4 cut(s) 356, 1320, 2417, 2480
TscAI CASTG 8 cut(s) 325, 358, 411, 1118, 1197, 1257, 1387, 2257
TseFI GTSAC 3 cut(s) 818, 1882, 2493
TseI GCWGC 3 cut(s) 149, 1652, 1724
Tsp45I GTSAC 3 cut(s) 818, 1882, 2493
TspGWI ACGGA 4 cut(s) 197, 1502, 1855, 2151
TspRI CASTG 8 cut(s) 325, 358, 411, 1118, 1197, 1257, 1387, 2257
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 1233
Vha464I CTTAAG 1 cut(s) 53
VpaK11BI GGWCC 3 cut(s) 184, 2465, 2506
XbaI TCTAGA 1 cut(s) 772
XcmI CCANNNNNNNNNTGG 2 cut(s) 1230, 1234
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 2127
XspI CTAG 8 cut(s) 206, 698, 773, 852, 1685, 1949, 1988, 2270
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.