Rorug03G0195400

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001584
Physical Location & Seq
Reverse (-)
17185846 .. 17186901
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0195400.1

Sequence Viewer

Length: 726 bp
ATGGCCCCAAGTAATACTATCTCCATGGCTCAACTCTGTTGTTGCATTCTCAAACCCATTCCTGGAGGGTTTTCATGGCGAAAACTTCCTGGATGGTTGAAATGGTTGGCCTTGTGCCGCTGCATAATCTGCCACATCGTTTTCTGGTTTGGAGCTTTCCTACTCATTTTCTGGCTGATCTTCCTTCCTGACGAGCCTAGATTTACTGTTACGAATGCCTCTCTAACCCAATTCAATTTCAACGGTAGTGACAACAAAAGCGGTACCAATCTTCACTACCACCTCGTCCTCAACATCACCATCAGAAACCCCAATAGAAGGGTCGGCATTGACTACCGTAGCATGACAGTGTCTGCTAGCTACAGAAAGAAGAAGTTTGGCCTCGTCAATTTGATGGATTCAGTACCATTTTACCAAGTCCACAAGAACACAACTTTTTTGCATCATCTGGTGCTTCAAGGGCAGCAGCAGTTGGTGAAATTCGGGAAGCGTGACATCTCCCAGTTTGACAAGGAGACTGCTGCTGGGGTTTATAGTATTACTGTGCAGGTTACTTTTCGGGTCAAGATCAGGTATGGAAAGTTTAAGGCCAACTTTTTGAAACCAGAAACCTACGATGACCAGCAGATCAACTGCGAGCTGAAACTACCTATAAGTATTACTAATGAGACATCTGGGGGTGGTTTCAAGGCTACACAGTGCGGCGATGTTGGTATATTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

241

Amino Acids

27.48

Weight (kDa)

9.58

Isoelectric Point (pI)

28.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 194 3.8e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 538
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 263
AciI CCGC 3 cut(s) 118, 261, 702
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 2 cut(s) 265, 405
AfiI CCNNNNNNNGG 2 cut(s) 62, 318
AgsI TTSAA 6 cut(s) 100, 235, 241, 458, 601, 688
AjnI CCWGG 2 cut(s) 61, 88
AluBI AGCT 3 cut(s) 155, 360, 640
AluI AGCT 3 cut(s) 155, 360, 640
Alw26I GTCTC 2 cut(s) 509, 662
AlwNI CAGNNNCTG 1 cut(s) 353
AoxI GGCC 4 cut(s) 3, 108, 379, 588
ApeKI GCWGC 4 cut(s) 120, 463, 466, 521
ApoI RAATTY 1 cut(s) 479
Asp718I GGTACC 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 289, 487
AsuNHI GCTAGC 1 cut(s) 356
BanI GGYRCC 1 cut(s) 263
BbvI GCAGC 4 cut(s) 107, 475, 478, 508
BccI CCATC 3 cut(s) 87, 308, 388
BciT130I CCWGG 2 cut(s) 63, 90
BcoDI GTCTC 2 cut(s) 509, 662
BfaI CTAG 2 cut(s) 198, 357
BfmI CTRYAG 1 cut(s) 361
BfuAI ACCTGC 1 cut(s) 538
BisI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 703
BlsI GCNGC 6 cut(s) 119, 122, 465, 468, 523, 704
Bme1390I CCNGG 2 cut(s) 63, 90
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 265
BmrFI CCNGG 2 cut(s) 63, 90
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 451
BmtI GCTAGC 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 496
BpmI CTGGAG 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 318
Bse1I ACTGG 1 cut(s) 502
BseBI CCWGG 2 cut(s) 63, 90
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 2 cut(s) 62, 318
BseNI ACTGG 1 cut(s) 502
BseXI GCAGC 4 cut(s) 107, 475, 478, 508
BseYI CCCAGC 1 cut(s) 524
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 4 cut(s) 5, 110, 381, 590
BshNI GGYRCC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 62, 318
BsmAI GTCTC 2 cut(s) 509, 662
BsmI GAATGC 2 cut(s) 45, 220
BsnI GGCC 4 cut(s) 5, 110, 381, 590
Bsp143I GATC 3 cut(s) 177, 567, 627
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 3 cut(s) 118, 261, 702
BspANI GGCC 4 cut(s) 5, 110, 381, 590
BspLI GGNNCC 2 cut(s) 6, 265
BspMI ACCTGC 1 cut(s) 538
BspOI GCTAGC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 3 cut(s) 177, 567, 627
BssT1I CCWWGG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 63, 90
Bst4CI ACNGT 6 cut(s) 208, 245, 338, 349, 544, 699
BstAPI GCANNNNNTGC 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 358, 638
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 3 cut(s) 180, 570, 630
BstMAI GTCTC 2 cut(s) 509, 662
BstMBI GATC 3 cut(s) 177, 567, 627
BstMWI GCNNNNNNNGC 2 cut(s) 129, 460
BstNI CCWGG 2 cut(s) 63, 90
BstSCI CCNGG 2 cut(s) 61, 88
BstSFI CTRYAG 1 cut(s) 361
BstV1I GCAGC 4 cut(s) 107, 475, 478, 508
BsuRI GGCC 4 cut(s) 5, 110, 381, 590
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 1 cut(s) 98
BtsIMutI CAGTG 2 cut(s) 354, 704
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 2 cut(s) 358, 638
CaiI CAGNNNCTG 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 264, 404
CviAII CATG 3 cut(s) 25, 75, 343
CviQI GTAC 2 cut(s) 264, 404
DpnI GATC 3 cut(s) 179, 569, 629
DpnII GATC 3 cut(s) 177, 567, 627
Eco130I CCWWGG 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 61, 88
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 3 cut(s) 28, 78, 346
FaiI YATR 8 cut(s) 26, 76, 125, 344, 534, 576, 653, 716
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FatI CATG 3 cut(s) 24, 74, 342
Fnu4HI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 703
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 703
FspBI CTAG 2 cut(s) 198, 357
GluI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 703
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 4 cut(s) 5, 110, 381, 590
Hin1II CATG 3 cut(s) 28, 78, 346
HinfI GANTC 1 cut(s) 398
HphI GGTGA 2 cut(s) 289, 487
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 3 cut(s) 188, 484, 565
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 2 cut(s) 194, 312
HpyCH4III ACNGT 6 cut(s) 208, 245, 338, 349, 544, 699
HpyCH4V TGCA 4 cut(s) 45, 123, 442, 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 460
Hsp92II CATG 3 cut(s) 28, 78, 346
KpnI GGTACC 1 cut(s) 267
Kzo9I GATC 3 cut(s) 177, 567, 627
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 4 cut(s) 107, 475, 478, 508
LweI GCATC 1 cut(s) 451
MaeI CTAG 2 cut(s) 198, 357
MaeIII GTNAC 4 cut(s) 208, 248, 491, 550
MalI GATC 3 cut(s) 179, 569, 629
MboI GATC 3 cut(s) 177, 567, 627
MboII GAAGA 3 cut(s) 172, 263, 382
MluCI AATT 4 cut(s) 230, 235, 388, 479
MnlI CCTC 5 cut(s) 59, 229, 293, 299, 392
MseI TTAA 2 cut(s) 585, 724
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 63, 90
Mva1269I GAATGC 2 cut(s) 45, 220
MvaI CCWGG 2 cut(s) 63, 90
MwoI GCNNNNNNNGC 2 cut(s) 129, 460
NcoI CCATGG 1 cut(s) 24
NdeII GATC 3 cut(s) 177, 567, 627
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 3 cut(s) 28, 78, 346
NlaIV GGNNCC 2 cut(s) 6, 265
NmuCI GTSAC 2 cut(s) 248, 491
PctI GAATGC 2 cut(s) 45, 220
PfeI GAWTC 1 cut(s) 398
PflFI GACNNNGTC 1 cut(s) 349
PfoI TCCNGGA 2 cut(s) 61, 88
PkrI GCNGC 6 cut(s) 119, 122, 465, 468, 523, 704
Psp6I CCWGG 2 cut(s) 61, 88
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 2 cut(s) 61, 88
PspN4I GGNNCC 2 cut(s) 6, 265
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 353
PsyI GACNNNGTC 1 cut(s) 349
RsaI GTAC 2 cut(s) 265, 405
RsaNI GTAC 2 cut(s) 264, 404
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 2 cut(s) 585, 724
SatI GCNGC 6 cut(s) 118, 121, 464, 467, 522, 703
Sau3AI GATC 3 cut(s) 177, 567, 627
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 63, 90
SetI ASST 8 cut(s) 157, 285, 362, 552, 575, 614, 642, 652
SfaNI GCATC 1 cut(s) 451
SfcI CTRYAG 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 347
Sse9I AATT 4 cut(s) 230, 235, 388, 479
SsiI CCGC 3 cut(s) 118, 261, 702
SspMI CTAG 2 cut(s) 198, 357
StyD4I CCNGG 2 cut(s) 61, 88
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 6 cut(s) 208, 245, 338, 349, 544, 699
TasI AATT 4 cut(s) 230, 235, 388, 479
TauI GCSGC 2 cut(s) 120, 705
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 2 cut(s) 585, 724
Tru9I TTAA 2 cut(s) 585, 724
TscAI CASTG 2 cut(s) 354, 704
TseFI GTSAC 2 cut(s) 248, 491
TseI GCWGC 4 cut(s) 120, 463, 466, 521
Tsp45I GTSAC 2 cut(s) 248, 491
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 2 cut(s) 354, 704
Tth111I GACNNNGTC 1 cut(s) 349
XapI RAATTY 1 cut(s) 479
XspI CTAG 2 cut(s) 198, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.