pycom02g25900

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005826
Physical Location & Seq
Reverse (-)
23804088 .. 23806569
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGGAGAATGATAACGATTTCACTTTTTGTCAGGTTGGTCCACCAGGCAACCAGGATGGTTATGAGACAAAGAAGCTCACTTCAGATATAGAAAACATTACCCTAAAGGATGGATTGTCAAATGGCAATAGTAGTAATGAGAATAGAGGTATTCTGTGGCAAGGTGGATTACCAGGAGGAGCCGCTTCCAAAAAACAGGAAACAGTTGGTTCTTTGTCTGTCAGCATCATTGATGCATCATCCATGAAAAAGAAATCTGAAGTTCCGAAGCAACTAAAATCAGGTGATGCTGGAAAGTCAGTGAAGCCTGCAACTCGTGCTAAAGTTCCTTTTGAGAAGGGGTATAGCCAAATGGATTGGCTCAAACTTACTCGAACACATCCTGACCTTGCAGGTTTAAAGGGACAATCAAATAAGAGGCTTATTTCACTGAATGAAGTTAAACAGCACCAAAAAGGAGATTCAATGTGGACTGTTCTAAAAGGCCGTGTGTACAATTTGTCTCCATACATGAGATTTCACCCCGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGAAAAGATTGCACATCTTTGTTCAATAAATACCATGCTTGGGTTAATGCTGAATTTTTACTCGAGAAATGTCTCGTTGGTACTTTAGACGATGGTGAAGGGCAACGTTCCAATCATTCGGAATCTTCGGCCCCCTCATGA

Protein Analysis

232

Amino Acids

25.23

Weight (kDa)

9.1

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 144 - 215 2e-23 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AciI CCGC 1 cut(s) 183
AclI AACGTT 1 cut(s) 661
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 2 cut(s) 66, 279
AfaI GTAC 2 cut(s) 494, 637
AfiI CCNNNNNNNGG 1 cut(s) 595
AgsI TTSAA 2 cut(s) 465, 580
AjnI CCWGG 3 cut(s) 43, 51, 172
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 3 cut(s) 59, 507, 632
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 2 cut(s) 484, 684
ApoI RAATTY 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 38, 685
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 3 cut(s) 296, 512, 662
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 315
BccI CCATC 3 cut(s) 50, 104, 641
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 3 cut(s) 45, 53, 174
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 3 cut(s) 59, 507, 632
BfuAI ACCTGC 1 cut(s) 383
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme1390I CCNGG 4 cut(s) 45, 53, 174, 525
Bme18I GGWCC 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 2 cut(s) 38, 685
BmiI GGNNCC 2 cut(s) 181, 687
BmrFI CCNGG 4 cut(s) 45, 53, 174, 525
BmsI GCATC 4 cut(s) 223, 234, 245, 277
BpuMI CCSGG 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 523
Bsc4I CCNNNNNNNGG 1 cut(s) 595
BseBI CCWGG 3 cut(s) 45, 53, 174
BseDI CCNNGG 1 cut(s) 523
BseGI GGATG 4 cut(s) 61, 115, 239, 379
BseLI CCNNNNNNNGG 1 cut(s) 595
BseRI GAGGAG 1 cut(s) 192
BshFI GGCC 2 cut(s) 486, 686
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 3 cut(s) 59, 507, 632
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 2 cut(s) 486, 686
BsoBI CYCGRG 1 cut(s) 617
Bsp1407I TGTACA 1 cut(s) 492
BspACI CCGC 1 cut(s) 183
BspANI GGCC 2 cut(s) 486, 686
BspHI TCATGA 1 cut(s) 692
BspLI GGNNCC 2 cut(s) 181, 687
BspMI ACCTGC 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 492
BssECI CCNNGG 1 cut(s) 523
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 45, 53, 174
Bst4CI ACNGT 2 cut(s) 205, 475
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 309
BstF5I GGATG 4 cut(s) 61, 115, 239, 379
BstMAI GTCTC 3 cut(s) 59, 507, 632
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 3 cut(s) 45, 53, 174
BstSCI CCNGG 4 cut(s) 43, 51, 172, 523
BsuRI GGCC 2 cut(s) 486, 686
BtsCI GGATG 4 cut(s) 61, 115, 239, 379
BtsIMutI CAGTG 2 cut(s) 306, 428
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 1 cut(s) 309
CciI TCATGA 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 38, 685
Csp6I GTAC 2 cut(s) 493, 636
CviAII CATG 4 cut(s) 244, 511, 590, 693
CviJI RGCY 9 cut(s) 76, 182, 307, 348, 361, 421, 486, 551, 686
CviKI_1 RGCY 9 cut(s) 76, 182, 307, 348, 361, 421, 486, 551, 686
CviQI GTAC 2 cut(s) 493, 636
DraI TTTAAA 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 38
Eco57I CTGAAG 2 cut(s) 66, 279
Eco88I CYCGRG 1 cut(s) 617
EcoRII CCWGG 3 cut(s) 43, 51, 172
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 4 cut(s) 247, 514, 593, 696
FaiI YATR 8 cut(s) 63, 89, 245, 345, 508, 512, 591, 694
FaqI GGGAC 1 cut(s) 417
FatI CATG 4 cut(s) 243, 510, 589, 692
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 4 cut(s) 68, 122, 226, 366
Fsp4HI GCNGC 1 cut(s) 183
GluI GCNGC 1 cut(s) 183
HaeIII GGCC 2 cut(s) 486, 686
HapII CCGG 1 cut(s) 525
Hin1II CATG 4 cut(s) 247, 514, 593, 696
HinfI GANTC 2 cut(s) 461, 677
HpaII CCGG 1 cut(s) 525
HphI GGTGA 3 cut(s) 296, 512, 662
Hpy166II GTNNAC 3 cut(s) 41, 471, 493
Hpy188I TCNGA 4 cut(s) 85, 259, 267, 676
Hpy188III TCNNGA 3 cut(s) 383, 619, 693
Hpy8I GTNNAC 3 cut(s) 41, 471, 493
HpyAV CCTTC 3 cut(s) 331, 541, 647
HpyCH4III ACNGT 2 cut(s) 205, 475
HpyCH4IV ACGT 1 cut(s) 661
HpyCH4V TGCA 4 cut(s) 236, 311, 392, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpySE526I ACGT 1 cut(s) 661
Hsp92II CATG 4 cut(s) 247, 514, 593, 696
LmnI GCTCC 1 cut(s) 179
LweI GCATC 4 cut(s) 223, 234, 245, 277
MaeII ACGT 1 cut(s) 661
MboII GAAGA 1 cut(s) 672
MluCI AATT 2 cut(s) 496, 608
MnlI CCTC 4 cut(s) 140, 170, 411, 521
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 3 cut(s) 398, 441, 600
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 4 cut(s) 45, 53, 174, 525
MvaI CCWGG 3 cut(s) 45, 53, 174
MwoI GCNNNNNNNGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 525
NlaIII CATG 4 cut(s) 247, 514, 593, 696
NlaIV GGNNCC 2 cut(s) 181, 687
NsiI ATGCAT 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 1 cut(s) 692
PfeI GAWTC 2 cut(s) 461, 677
PkrI GCNGC 1 cut(s) 184
Psp1406I AACGTT 1 cut(s) 661
Psp6I CCWGG 3 cut(s) 43, 51, 172
PspGI CCWGG 3 cut(s) 43, 51, 172
PspN4I GGNNCC 2 cut(s) 181, 687
PspPI GGNCC 2 cut(s) 38, 685
RsaI GTAC 2 cut(s) 494, 637
RsaNI GTAC 2 cut(s) 493, 636
SaqAI TTAA 3 cut(s) 398, 441, 600
SatI GCNGC 1 cut(s) 183
Sau96I GGNCC 2 cut(s) 38, 685
ScrFI CCNGG 4 cut(s) 45, 53, 174, 525
SetI ASST 9 cut(s) 36, 78, 151, 166, 286, 390, 397, 532, 664
SfaNI GCATC 4 cut(s) 223, 234, 245, 277
Sfr274I CTCGAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 38
SlaI CTCGAG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 2 cut(s) 496, 608
SsiI CCGC 1 cut(s) 183
StyD4I CCNGG 4 cut(s) 43, 51, 172, 523
TaaI ACNGT 2 cut(s) 205, 475
TaiI ACGT 1 cut(s) 664
TaqI TCGA 2 cut(s) 373, 618
TasI AATT 2 cut(s) 496, 608
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 2 cut(s) 461, 677
Tru1I TTAA 3 cut(s) 398, 441, 600
Tru9I TTAA 3 cut(s) 398, 441, 600
TscAI CASTG 2 cut(s) 306, 435
TspDTI ATGAA 2 cut(s) 260, 450
TspRI CASTG 2 cut(s) 306, 435
VpaK11BI GGWCC 1 cut(s) 38
XapI RAATTY 1 cut(s) 608
XhoI CTCGAG 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.