pycom02g25980

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008425
Physical Location & Seq
Reverse (-)
23851913 .. 23857518
5606 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1530 bp
ATGATTGTGGGCTCCCGGGTAGGGAGTTTGATGGCCCACAAAGAAGTGCCCTATCAAGTTATCAGCCTCCCTCCCGAGAGTCCATGGAGCCAACAAACACAACCAGCAGGAGCCTCTGAGATTCTCTCTGAACGTGAGTTTAACAAATCCAGACCCATAAGAAAAATGAAGTTTCCAACTTCTTCTTCGTATTCGTATTCCTCTGTTACTCCTATTCATTCGGTTTTTCTTCGCACAAAAAACCGTGGCAAATTGTCGTTTAATCTATTTCAGGGCACTTCTTCTCCAACAACCACCGCATCCACCACCACTTCCACTTCCACTTCCTCCTCCTCCTCTCAAAACCATCAACTCCTCAGCGGTAGCATACCCGCCGCCGCCATGGTTTCTTCTCCCGCCACCCACCCCCACCACGCCCACTACCCTCCTCCTTTGTTTAGTGTTGCCCCGATGATGGAGTGGACTGACAATCACTACAGGACTTTGGCCCGGCTTATTTCGAAGAATGCCTGGCTCTACACTGAGATGCTTGCTGCTGAAACCATTGTTTACCAGAAGGATAATCTGGACAGATTCTTGGAATATTCTCCAGAACAACATCCTATTGTTCTTCAAATTGGCGGGAATAATTTGGAAAACCTCGCCAAAGCGACTGAGCTTGCTAACCCTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCGAGTCCAAAAGTAGCTGGGCATGGGTGCTTTGGTGCTCGTCTTATGCTTGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTAATTGCTGCGCACACAGATGTACCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCATGATTCATATAACGAGCTCTGTGATTTTATTTACAAGGTTTCTTCTCAATCTCCAACTAGGCATTTCATAATACATTCACGGAAGGCACTGCTCAATGGAATTAGCCCGGCTGAAAATCGAAGTATTCCGCCTCTTAAATATGAGTACTTTTATGGCCTCTTGCGTGACTTTCCAGACTTGAGATTTACAATAAATGGGGGCATAAACACCGTTGAGGAGGTCAATGCAGCAAGAAGGGCAGGAGCTCATGGTGTAATGGTTGGACGCGCTGCATATAATAAACCATGGCATACTCTGGGACATGTTGATACTGTAGTTTATGGAGCACCAAGCAGTGGTGTAACGCGGCGTCAGATACTGGAGAAGTTTCAAGTGTATGGAGATACTGCTGTGGGAAGATATGGACGCAAACCAACTGTGCGAGATGTAGCAAGGCCCTTGCTGGGTCTTTTCCATTCGGAACCTGGGAATGGTCTATGGAAACGCAAAGCTGATGCTGCTTTCCTGCATTGCACGACTATGAAAGAGTTTTTTGATGAAACGCTTGTAGCACTTCCTGACTATGTTTTGGATAAACCTATTGGGGAGCCACCATCAGGCAGTGACGATCCTTTCGCGAACATACACAGTTTGCTACCTCCGGCGTATGAATCAAGGGAACTTGAACTGCAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

510

Amino Acids

56.39

Weight (kDa)

8.1

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 148 - 456 5.8e-59 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 798
AccB7I CCANNNNNTGG 1 cut(s) 1184
AccII CGCG 3 cut(s) 1116, 1195, 1466
AciI CCGC 9 cut(s) 297, 360, 372, 375, 378, 396, 621, 977, 1195
AclWI GGATC 2 cut(s) 752, 1451
AcyI GRCGYC 1 cut(s) 1198
AfaI GTAC 2 cut(s) 810, 995
AfiI CCNNNNNNNGG 6 cut(s) 21, 454, 1184, 1292, 1319, 1445
AflIII ACRYGT 1 cut(s) 1150
AgsI TTSAA 3 cut(s) 614, 1220, 1514
AjnI CCWGG 2 cut(s) 509, 1312
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 5 cut(s) 658, 722, 865, 1094, 1340
AluI AGCT 5 cut(s) 658, 722, 865, 1094, 1340
Alw21I GWGCWC 4 cut(s) 745, 867, 1096, 1177
AlwI GGATC 2 cut(s) 752, 1451
AlwNI CAGNNNCTG 1 cut(s) 1207
Ama87I CYCGRG 2 cut(s) 15, 74
AoxI GGCC 4 cut(s) 33, 486, 1003, 1283
ApeKI GCWGC 5 cut(s) 533, 794, 1076, 1118, 1346
ArsI GACNNNNNNTTYG 2 cut(s) 1445, 1477
AspLEI GCGC 2 cut(s) 799, 1118
AspS9I GGNCC 3 cut(s) 34, 487, 1284
AsuC2I CCSGG 4 cut(s) 16, 17, 490, 956
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 2 cut(s) 15, 74
BaeGI GKGCMC 2 cut(s) 51, 278
BaeI ACNNNNGTAYC 6 cut(s) 792, 825, 1149, 1182, 1224, 1257
BanII GRGCYC 3 cut(s) 14, 867, 1096
Bbv12I GWGCWC 4 cut(s) 745, 867, 1096, 1177
BbvCI CCTCAGC 1 cut(s) 356
BbvI GCAGC 5 cut(s) 520, 781, 1088, 1105, 1333
BccI CCATC 4 cut(s) 25, 354, 448, 1450
BciT130I CCWGG 2 cut(s) 511, 1314
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 4 cut(s) 16, 17, 490, 956
BfaI CTAG 1 cut(s) 906
BfmI CTRYAG 2 cut(s) 475, 1161
BisI GCNGC 8 cut(s) 375, 378, 534, 795, 1077, 1119, 1196, 1347
BlsI GCNGC 8 cut(s) 376, 379, 535, 796, 1078, 1120, 1197, 1348
BmcAI AGTACT 1 cut(s) 995
Bme1390I CCNGG 6 cut(s) 16, 17, 490, 511, 956, 1314
BmeT110I CYCGRG 2 cut(s) 15, 74
BmgT120I GGNCC 3 cut(s) 34, 487, 1284
BmiI GGNNCC 5 cut(s) 13, 89, 112, 1311, 1437
BmrFI CCNGG 6 cut(s) 16, 17, 490, 511, 956, 1314
BmsI GCATC 3 cut(s) 308, 516, 1333
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpmI CTGGAG 2 cut(s) 573, 1229
Bpu10I CCTNAGC 1 cut(s) 356
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 1048
BpuMI CCSGG 4 cut(s) 16, 17, 490, 956
BsaHI GRCGYC 1 cut(s) 1198
BsaJI CCNNGG 7 cut(s) 15, 83, 244, 381, 774, 1133, 1313
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 454, 1184, 1292, 1319, 1445
Bse1I ACTGG 1 cut(s) 1212
Bse3DI GCAATG 1 cut(s) 1357
BseBI CCWGG 2 cut(s) 511, 1314
BseDI CCNNGG 7 cut(s) 15, 83, 244, 381, 774, 1133, 1313
BseGI GGATG 3 cut(s) 299, 598, 707
BseLI CCNNNNNNNGG 6 cut(s) 21, 454, 1184, 1292, 1319, 1445
BseMI GCAATG 1 cut(s) 1357
BseMII CTCAG 4 cut(s) 108, 370, 513, 645
BseNI ACTGG 1 cut(s) 1212
BseRI GAGGAG 6 cut(s) 319, 322, 325, 344, 417, 1079
BseSI GKGCMC 2 cut(s) 51, 278
BseXI GCAGC 5 cut(s) 520, 781, 1088, 1105, 1333
BseYI CCCAGC 2 cut(s) 722, 1291
Bsh1236I CGCG 3 cut(s) 1116, 1195, 1466
BshFI GGCC 4 cut(s) 35, 488, 1005, 1285
BsiHKAI GWGCWC 4 cut(s) 745, 867, 1096, 1177
BsiHKCI CYCGRG 2 cut(s) 15, 74
BsiSI CCGG 4 cut(s) 16, 490, 956, 1490
BslFI GGGAC 1 cut(s) 1161
BslI CCNNNNNNNGG 6 cut(s) 21, 454, 1184, 1292, 1319, 1445
BsmFI GGGAC 1 cut(s) 1161
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 4 cut(s) 35, 488, 1005, 1285
BsoBI CYCGRG 2 cut(s) 15, 74
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 7 cut(s) 14, 51, 278, 745, 867, 1096, 1177
Bsp143I GATC 4 cut(s) 684, 757, 844, 1456
Bsp19I CCATGG 3 cut(s) 83, 381, 1133
Bsp68I TCGCGA 1 cut(s) 1466
BspACI CCGC 9 cut(s) 297, 360, 372, 375, 378, 396, 621, 977, 1195
BspANI GGCC 4 cut(s) 35, 488, 1005, 1285
BspCNI CTCAG 4 cut(s) 109, 369, 514, 646
BspFNI CGCG 3 cut(s) 1116, 1195, 1466
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 5 cut(s) 13, 89, 112, 1311, 1437
BspPI GGATC 2 cut(s) 752, 1451
BspT104I TTCGAA 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 1357
BsrI ACTGG 1 cut(s) 1212
BssECI CCNNGG 7 cut(s) 15, 83, 244, 381, 774, 1133, 1313
BssMI GATC 4 cut(s) 684, 757, 844, 1456
BssNI GRCGYC 1 cut(s) 1198
BssT1I CCWWGG 3 cut(s) 83, 381, 1133
Bst2UI CCWGG 2 cut(s) 511, 1314
Bst4CI ACNGT 5 cut(s) 245, 1060, 1162, 1267, 1478
BstACI GRCGYC 1 cut(s) 1198
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 531, 660
BstDEI CTNAG 5 cut(s) 117, 356, 522, 654, 1527
BstDSI CCRYGG 4 cut(s) 83, 244, 381, 1133
BstF5I GGATG 3 cut(s) 299, 598, 707
BstFNI CGCG 3 cut(s) 1116, 1195, 1466
BstHHI GCGC 2 cut(s) 799, 1118
BstKTI GATC 4 cut(s) 687, 760, 847, 1459
BstMBI GATC 4 cut(s) 684, 757, 844, 1456
BstMWI GCNNNNNNNGC 2 cut(s) 1085, 1346
BstNI CCWGG 2 cut(s) 511, 1314
BstNSI RCATGY 1 cut(s) 1154
BstSCI CCNGG 6 cut(s) 14, 15, 488, 509, 954, 1312
BstSFI CTRYAG 2 cut(s) 475, 1161
BstSLI GKGCMC 2 cut(s) 51, 278
BstUI CGCG 3 cut(s) 1116, 1195, 1466
BstV1I GCAGC 5 cut(s) 520, 781, 1088, 1105, 1333
BsuRI GGCC 4 cut(s) 35, 488, 1005, 1285
BtgI CCRYGG 4 cut(s) 83, 244, 381, 1133
BtsCI GGATG 3 cut(s) 299, 598, 707
BtsI GCAGTG 3 cut(s) 935, 1189, 1456
BtsIMutI CAGTG 4 cut(s) 519, 935, 1189, 1456
BtuMI TCGCGA 1 cut(s) 1466
Cac8I GCNNGC 2 cut(s) 531, 660
CaiI CAGNNNCTG 1 cut(s) 1207
CciI TCATGA 1 cut(s) 847
CfoI GCGC 2 cut(s) 799, 1118
Cfr13I GGNCC 3 cut(s) 34, 487, 1284
Cfr9I CCCGGG 1 cut(s) 15
CseI GACGC 3 cut(s) 1122, 1187, 1263
Csp6I GTAC 2 cut(s) 809, 994
CviAII CATG 7 cut(s) 84, 382, 728, 848, 1097, 1134, 1151
CviQI GTAC 2 cut(s) 809, 994
DdeI CTNAG 5 cut(s) 117, 356, 522, 654, 1527
DpnI GATC 4 cut(s) 686, 759, 846, 1458
DpnII GATC 4 cut(s) 684, 757, 844, 1456
EciI GGCGGA 1 cut(s) 966
Ecl136II GAGCTC 2 cut(s) 865, 1094
Eco130I CCWWGG 3 cut(s) 83, 381, 1133
Eco24I GRGCYC 3 cut(s) 14, 867, 1096
Eco53kI GAGCTC 2 cut(s) 865, 1094
Eco88I CYCGRG 2 cut(s) 15, 74
EcoICRI GAGCTC 2 cut(s) 865, 1094
EcoO109I RGGNCCY 1 cut(s) 1284
EcoRII CCWGG 2 cut(s) 509, 1312
EcoT14I CCWWGG 3 cut(s) 83, 381, 1133
EcoT38I GRGCYC 3 cut(s) 14, 867, 1096
ErhI CCWWGG 3 cut(s) 83, 381, 1133
FaeI CATG 7 cut(s) 87, 385, 731, 851, 1100, 1137, 1154
FaqI GGGAC 1 cut(s) 1161
FatI CATG 7 cut(s) 83, 381, 727, 847, 1096, 1133, 1150
FauI CCCGC 3 cut(s) 379, 403, 614
FbaI TGATCA 1 cut(s) 844
Fnu4HI GCNGC 8 cut(s) 375, 378, 534, 795, 1077, 1119, 1196, 1347
FokI GGATG 3 cut(s) 286, 585, 714
FriOI GRGCYC 3 cut(s) 14, 867, 1096
Fsp4HI GCNGC 8 cut(s) 375, 378, 534, 795, 1077, 1119, 1196, 1347
FspBI CTAG 1 cut(s) 906
FspI TGCGCA 1 cut(s) 798
GlaI GCGC 2 cut(s) 798, 1117
GluI GCNGC 8 cut(s) 375, 378, 534, 795, 1077, 1119, 1196, 1347
GsaI CCCAGC 2 cut(s) 726, 1295
GsuI CTGGAG 2 cut(s) 573, 1229
HaeIII GGCC 4 cut(s) 35, 488, 1005, 1285
HapII CCGG 4 cut(s) 16, 490, 956, 1490
HgaI GACGC 3 cut(s) 1122, 1187, 1263
HhaI GCGC 2 cut(s) 799, 1118
Hin1I GRCGYC 1 cut(s) 1198
Hin1II CATG 7 cut(s) 87, 385, 731, 851, 1100, 1137, 1154
Hin6I GCGC 2 cut(s) 797, 1116
HinP1I GCGC 2 cut(s) 797, 1116
HinfI GANTC 6 cut(s) 79, 121, 573, 709, 851, 1499
HpaII CCGG 4 cut(s) 16, 490, 956, 1490
Hpy166II GTNNAC 2 cut(s) 462, 550
Hpy188I TCNGA 5 cut(s) 118, 130, 708, 1203, 1309
Hpy188III TCNNGA 8 cut(s) 74, 150, 566, 590, 848, 1022, 1406, 1465
Hpy8I GTNNAC 2 cut(s) 462, 550
HpyAV CCTTC 3 cut(s) 550, 925, 1077
HpyCH4III ACNGT 5 cut(s) 245, 1060, 1162, 1267, 1478
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 5 cut(s) 1076, 1121, 1357, 1362, 1519
HpyF10VI GCNNNNNNNGC 2 cut(s) 1085, 1346
HpyF3I CTNAG 5 cut(s) 117, 356, 522, 654, 1527
HpySE526I ACGT 1 cut(s) 133
Hsp92I GRCGYC 1 cut(s) 1198
Hsp92II CATG 7 cut(s) 87, 385, 731, 851, 1100, 1137, 1154
HspAI GCGC 2 cut(s) 797, 1116
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 4 cut(s) 684, 757, 844, 1456
LmnI GCTCC 6 cut(s) 17, 87, 110, 1091, 1172, 1435
Lsp1109I GCAGC 5 cut(s) 520, 781, 1088, 1105, 1333
LweI GCATC 3 cut(s) 308, 516, 1333
MaeI CTAG 1 cut(s) 906
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 4 cut(s) 205, 1013, 1189, 1451
MalI GATC 4 cut(s) 686, 759, 846, 1458
MboI GATC 4 cut(s) 684, 757, 844, 1456
MboII GAAGA 9 cut(s) 174, 177, 221, 273, 381, 514, 602, 882, 1257
MhlI GDGCHC 7 cut(s) 14, 51, 278, 745, 867, 1096, 1177
MluCI AATT 8 cut(s) 251, 615, 628, 688, 694, 789, 831, 948
MlyI GAGTC 2 cut(s) 88, 718
MmeI TCCRAC 4 cut(s) 200, 311, 926, 1090
MseI TTAA 5 cut(s) 141, 261, 693, 822, 984
MslI CAYNNNNRTG 3 cut(s) 524, 804, 1367
MspA1I CMGCKG 1 cut(s) 360
MspI CCGG 4 cut(s) 16, 490, 956, 1490
MspR9I CCNGG 6 cut(s) 16, 17, 490, 511, 956, 1314
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 2 cut(s) 511, 1314
MvnI CGCG 3 cut(s) 1116, 1195, 1466
MwoI GCNNNNNNNGC 2 cut(s) 1085, 1346
NciI CCSGG 4 cut(s) 16, 17, 490, 956
NcoI CCATGG 3 cut(s) 83, 381, 1133
NdeII GATC 4 cut(s) 684, 757, 844, 1456
NlaIII CATG 7 cut(s) 87, 385, 731, 851, 1100, 1137, 1154
NlaIV GGNNCC 5 cut(s) 13, 89, 112, 1311, 1437
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 2 cut(s) 1013, 1451
NruI TCGCGA 1 cut(s) 1466
NsbI TGCGCA 1 cut(s) 798
NspI RCATGY 1 cut(s) 1154
NspV TTCGAA 1 cut(s) 500
PagI TCATGA 1 cut(s) 847
PciI ACATGT 1 cut(s) 1150
PctI GAATGC 1 cut(s) 511
PfeI GAWTC 4 cut(s) 121, 573, 851, 1499
PflMI CCANNNNNTGG 1 cut(s) 1184
PkrI GCNGC 8 cut(s) 376, 379, 535, 796, 1078, 1120, 1197, 1348
PleI GAGTC 2 cut(s) 87, 717
PpsI GAGTC 2 cut(s) 87, 717
PscI ACATGT 1 cut(s) 1150
Psp124BI GAGCTC 2 cut(s) 867, 1096
Psp6I CCWGG 2 cut(s) 509, 1312
PspFI CCCAGC 2 cut(s) 722, 1291
PspGI CCWGG 2 cut(s) 509, 1312
PspN4I GGNNCC 5 cut(s) 13, 89, 112, 1311, 1437
PspPI GGNCC 3 cut(s) 34, 487, 1284
PstNI CAGNNNCTG 1 cut(s) 1207
RruI TCGCGA 1 cut(s) 1466
RsaI GTAC 2 cut(s) 810, 995
RsaNI GTAC 2 cut(s) 809, 994
RseI CAYNNNNRTG 3 cut(s) 524, 804, 1367
SacI GAGCTC 2 cut(s) 867, 1096
SaqAI TTAA 5 cut(s) 141, 261, 693, 822, 984
SatI GCNGC 8 cut(s) 375, 378, 534, 795, 1077, 1119, 1196, 1347
Sau3AI GATC 4 cut(s) 684, 757, 844, 1456
Sau96I GGNCC 3 cut(s) 34, 487, 1284
ScaI AGTACT 1 cut(s) 995
SchI GAGTC 2 cut(s) 88, 718
ScrFI CCNGG 6 cut(s) 16, 17, 490, 511, 956, 1314
SduI GDGCHC 7 cut(s) 14, 51, 278, 745, 867, 1096, 1177
SfaNI GCATC 3 cut(s) 308, 516, 1333
SfcI CTRYAG 2 cut(s) 475, 1161
SfuI TTCGAA 1 cut(s) 500
SmaI CCCGGG 1 cut(s) 17
SmiMI CAYNNNNRTG 3 cut(s) 524, 804, 1367
SmlI CTYRAG 1 cut(s) 1027
SmoI CTYRAG 1 cut(s) 1027
Sse9I AATT 8 cut(s) 251, 615, 628, 688, 694, 789, 831, 948
SsiI CCGC 9 cut(s) 297, 360, 372, 375, 378, 396, 621, 977, 1195
SspI AATATT 1 cut(s) 584
SspMI CTAG 1 cut(s) 906
SstI GAGCTC 2 cut(s) 867, 1096
StyD4I CCNGG 6 cut(s) 14, 15, 488, 509, 954, 1312
StyI CCWWGG 3 cut(s) 83, 381, 1133
TaaI ACNGT 5 cut(s) 245, 1060, 1162, 1267, 1478
TaiI ACGT 1 cut(s) 136
TaqI TCGA 3 cut(s) 500, 829, 967
TasI AATT 8 cut(s) 251, 615, 628, 688, 694, 789, 831, 948
TatI WGTACW 1 cut(s) 993
TauI GCSGC 3 cut(s) 377, 380, 1198
TfiI GAWTC 4 cut(s) 121, 573, 851, 1499
Tru1I TTAA 5 cut(s) 141, 261, 693, 822, 984
Tru9I TTAA 5 cut(s) 141, 261, 693, 822, 984
TscAI CASTG 4 cut(s) 526, 942, 1189, 1456
TseFI GTSAC 2 cut(s) 1013, 1451
TseI GCWGC 5 cut(s) 533, 794, 1076, 1118, 1346
Tsp45I GTSAC 2 cut(s) 1013, 1451
TspDTI ATGAA 7 cut(s) 182, 206, 843, 904, 1385, 1401, 1512
TspGWI ACGGA 1 cut(s) 943
TspMI CCCGGG 1 cut(s) 15
TspRI CASTG 4 cut(s) 526, 942, 1189, 1456
Van91I CCANNNNNTGG 1 cut(s) 1184
XceI RCATGY 1 cut(s) 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 1310
XmaI CCCGGG 1 cut(s) 15
XspI CTAG 1 cut(s) 906
ZrmI AGTACT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.