pycom02g26680

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005345
Physical Location & Seq
Reverse (-)
24483492 .. 24487171
3680 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGATCTTTGTTGATGGGCAACTCTCTACCACAGACTCTTCTGCATCGCCTCAGGGACGGGATATTCTGGTTGCTCTATTGGAACACCCTGCTTTGGTAGAAGCCTCAGCTTCGTTCAAGGCTATCCCGGAGAGGAGATTCTCTGTACGTGAAGAGTCAGTTGTGGAGGCAACGCCACCCGGTAAATGGGTTTATTTGTTCCAGAGAGAATTTGCAACTGTTGATCCGGCATTTGTGGATTTTGTTGGAACTGATGAAGCAACGACCTGTATCGGTGTGGCCATTCGGAATCCGAGAAATGGAATGACCTCAGTTGCTCATATGGATTCTCCAAACATTGTGGATATGGGTCTGTCGCAGATGTTGTCACTCCTTGTTGACCATAACAAGGACATGGAGTTAGATCATGGTAACTACAACACTAAATCAGAAAGTGAGGAAAACATGGATGGTTATTCCTTTCCTTTAGGTGCCAAAATTGTTGAAACTTTATGGAAGAGACCAGAGAAATTCCATATACGTACTCTCTGCATTCTTGGGCATAACACTAGAAGGGATCCCGAGGGAAACGCGTACCCAAACTTCAATGGGTTCATGGTAGGAACTTCCAATGGATCTATTACCCCAGCTATCTTTGACAAAACCTCAAGATGTCCTGATGAAATTGTCAGGAGAATTCGAGTTTCTGTATCATACAAGGACCCCAGTTGGAAAGGAAGGTTACTGGAAACATATGAAACTCAAACTGATCGATTCAAGATAGCTCCATGTTGCTGGACACTGCAGCAATTACACTTTGCTTTATCATTACAACATTATTCTGATTCAGAAATCCTCATCATGTGTTCTACTTCACCTTCTGCTGAGAGCCCAGATTTTGTGGCGAATATGAGAAGGCATTGGGACTACCTAATCAAACACCCAGATTGGAGAGAAACCTTTCCTATGAAGCAGCCACGCATATTTGAGAGGACTGCCGAAGGAGGCTGGAGAAAGCCACAAGGTTCTGATACATTAATCAGTAGGAGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

345

Amino Acids

39.03

Weight (kDa)

5.84

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 57 - 331 6.1e-76 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 470
AccII CGCG 1 cut(s) 572
AclWI GGATC 4 cut(s) 218, 551, 564, 622
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 209, 509, 675
AfaI GTAC 3 cut(s) 147, 523, 575
AfiI CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 4 cut(s) 118, 485, 586, 757
AluBI AGCT 3 cut(s) 110, 629, 764
AluI AGCT 3 cut(s) 110, 629, 764
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 4 cut(s) 218, 551, 564, 622
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 784, 952
ApoI RAATTY 3 cut(s) 209, 509, 675
AseI ATTAAT 1 cut(s) 1016
Asp700I GAANNNNTTC 1 cut(s) 939
AspS9I GGNCC 1 cut(s) 700
AsuC2I CCSGG 2 cut(s) 128, 180
AsuHPI GGTGA 1 cut(s) 846
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 1 cut(s) 700
AxyI CCTNAGG 1 cut(s) 51
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 872
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbvCI CCTCAGC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 796, 964
BccI CCATC 2 cut(s) 8, 443
BcnI CCSGG 2 cut(s) 128, 180
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 2 cut(s) 549, 1033
BfmI CTRYAG 1 cut(s) 782
BisI GCNGC 2 cut(s) 785, 953
BlsI GCNGC 2 cut(s) 786, 954
Bme1390I CCNGG 2 cut(s) 128, 180
Bme18I GGWCC 1 cut(s) 700
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 700
BmiI GGNNCC 3 cut(s) 472, 558, 702
BmrFI CCNGG 2 cut(s) 128, 180
BmrI ACTGGG 1 cut(s) 699
BmsI GCATC 1 cut(s) 53
BmuI ACTGGG 1 cut(s) 699
BpmI CTGGAG 1 cut(s) 1009
Bpu10I CCTNAGC 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 2 cut(s) 128, 180
Bsa29I ATCGAT 1 cut(s) 751
BsaAI YACGTR 2 cut(s) 149, 521
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 561
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
Bse1I ACTGG 2 cut(s) 705, 729
Bse21I CCTNAGG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
BseMII CTCAG 4 cut(s) 65, 120, 324, 855
BseNI ACTGG 2 cut(s) 705, 729
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 2 cut(s) 796, 964
BseYI CCCAGC 1 cut(s) 625
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 751
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 3 cut(s) 128, 180, 227
BslFI GGGAC 2 cut(s) 69, 917
BslI CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
BsmAI GTCTC 1 cut(s) 493
BsmFI GGGAC 2 cut(s) 69, 917
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 872
Bsp143I GATC 6 cut(s) 3, 223, 403, 556, 614, 748
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 4 cut(s) 64, 119, 323, 856
BspDI ATCGAT 1 cut(s) 751
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 3 cut(s) 472, 558, 702
BspMAI CTGCAG 1 cut(s) 786
BspPI GGATC 4 cut(s) 218, 551, 564, 622
BspT107I GGYRCC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 493
BsrI ACTGG 2 cut(s) 705, 729
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 6 cut(s) 3, 223, 403, 556, 614, 748
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 3 cut(s) 43, 147, 491
BstBAI YACGTR 2 cut(s) 149, 521
BstDEI CTNAG 4 cut(s) 51, 106, 310, 864
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 1 cut(s) 572
BstKTI GATC 6 cut(s) 6, 226, 406, 559, 617, 751
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 6 cut(s) 3, 223, 403, 556, 614, 748
BstSCI CCNGG 2 cut(s) 126, 178
BstSFI CTRYAG 1 cut(s) 782
BstSNI TACGTA 1 cut(s) 521
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 2 cut(s) 796, 964
BstX2I RGATCY 2 cut(s) 556, 614
BstXI CCANNNNNNTGG 1 cut(s) 774
BstYI RGATCY 2 cut(s) 556, 614
Bsu15I ATCGAT 1 cut(s) 751
Bsu36I CCTNAGG 1 cut(s) 51
BsuRI GGCC 1 cut(s) 281
BsuTUI ATCGAT 1 cut(s) 751
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 454
BtsI GCAGTG 1 cut(s) 779
BtsIMutI CAGTG 1 cut(s) 779
Cfr13I GGNCC 1 cut(s) 700
ClaI ATCGAT 1 cut(s) 751
Csp6I GTAC 3 cut(s) 146, 522, 574
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 6 cut(s) 394, 407, 445, 595, 768, 841
CviQI GTAC 3 cut(s) 146, 522, 574
DdeI CTNAG 4 cut(s) 51, 106, 310, 864
DpnI GATC 6 cut(s) 5, 225, 405, 558, 616, 750
DpnII GATC 6 cut(s) 3, 223, 403, 556, 614, 748
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 3 cut(s) 43, 147, 491
EarI CTCTTC 3 cut(s) 43, 147, 491
Eco105I TACGTA 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 872
Eco31I GGTCTC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 700
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 872
FaeI CATG 6 cut(s) 397, 410, 448, 598, 771, 844
FaqI GGGAC 2 cut(s) 69, 917
FatI CATG 6 cut(s) 393, 406, 444, 594, 767, 840
FauNDI CATATG 2 cut(s) 321, 733
Fnu4HI GCNGC 2 cut(s) 785, 953
FokI GGATG 1 cut(s) 461
FriOI GRGCYC 1 cut(s) 872
Fsp4HI GCNGC 2 cut(s) 785, 953
FspBI CTAG 2 cut(s) 549, 1033
GluI GCNGC 2 cut(s) 785, 953
GsaI CCCAGC 1 cut(s) 629
GsuI CTGGAG 1 cut(s) 1009
HaeIII GGCC 1 cut(s) 281
HapII CCGG 3 cut(s) 128, 180, 227
Hin1II CATG 6 cut(s) 397, 410, 448, 598, 771, 844
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 7 cut(s) 35, 138, 155, 289, 326, 753, 824
HpaII CCGG 3 cut(s) 128, 180, 227
HphI GGTGA 1 cut(s) 846
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 6 cut(s) 288, 294, 430, 823, 829, 1009
Hpy188III TCNNGA 6 cut(s) 202, 560, 648, 656, 670, 757
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 5 cut(s) 546, 711, 867, 888, 974
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 2 cut(s) 148, 520
HpyCH4V TGCA 4 cut(s) 44, 215, 531, 784
HpyF3I CTNAG 4 cut(s) 51, 106, 310, 864
HpySE526I ACGT 2 cut(s) 148, 520
Hsp92II CATG 6 cut(s) 397, 410, 448, 598, 771, 844
Kzo9I GATC 6 cut(s) 3, 223, 403, 556, 614, 748
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 2 cut(s) 796, 964
LweI GCATC 1 cut(s) 53
MaeI CTAG 2 cut(s) 549, 1033
MaeII ACGT 2 cut(s) 148, 520
MaeIII GTNAC 3 cut(s) 366, 410, 720
MalI GATC 6 cut(s) 5, 225, 405, 558, 616, 750
MboI GATC 6 cut(s) 3, 223, 403, 556, 614, 748
MboII GAAGA 3 cut(s) 30, 164, 508
MflI RGATCY 2 cut(s) 556, 614
MhlI GDGCHC 1 cut(s) 872
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 6 cut(s) 209, 477, 509, 663, 675, 788
MluI ACGCGT 1 cut(s) 570
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 2 cut(s) 29, 164
MmeI TCCRAC 2 cut(s) 226, 689
Mox20I TGGCCA 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 939
MscI TGGCCA 1 cut(s) 281
MseI TTAA 1 cut(s) 1016
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 3 cut(s) 128, 180, 227
MspR9I CCNGG 2 cut(s) 128, 180
Mva1269I GAATGC 1 cut(s) 531
MvnI CGCG 1 cut(s) 572
NciI CCSGG 2 cut(s) 128, 180
NdeI CATATG 2 cut(s) 321, 733
NdeII GATC 6 cut(s) 3, 223, 403, 556, 614, 748
NlaIII CATG 6 cut(s) 397, 410, 448, 598, 771, 844
NlaIV GGNNCC 3 cut(s) 472, 558, 702
NmuCI GTSAC 1 cut(s) 366
PctI GAATGC 1 cut(s) 531
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 5 cut(s) 138, 289, 326, 753, 824
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 2 cut(s) 786, 954
PleI GAGTC 2 cut(s) 29, 163
PpsI GAGTC 2 cut(s) 29, 163
Ppu21I YACGTR 2 cut(s) 149, 521
PpuMI RGGWCCY 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 625
PspN4I GGNNCC 3 cut(s) 472, 558, 702
PspPI GGNCC 1 cut(s) 700
PspPPI RGGWCCY 1 cut(s) 700
PstI CTGCAG 1 cut(s) 786
PsuI RGATCY 2 cut(s) 556, 614
RsaI GTAC 3 cut(s) 147, 523, 575
RsaNI GTAC 3 cut(s) 146, 522, 574
SaqAI TTAA 1 cut(s) 1016
SatI GCNGC 2 cut(s) 785, 953
Sau3AI GATC 6 cut(s) 3, 223, 403, 556, 614, 748
Sau96I GGNCC 1 cut(s) 700
SchI GAGTC 2 cut(s) 29, 164
ScrFI CCNGG 2 cut(s) 128, 180
SduI GDGCHC 1 cut(s) 872
SfaNI GCATC 1 cut(s) 53
SfcI CTRYAG 1 cut(s) 782
SinI GGWCC 1 cut(s) 700
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SnaBI TACGTA 1 cut(s) 521
Sse9I AATT 6 cut(s) 209, 477, 509, 663, 675, 788
SspMI CTAG 2 cut(s) 549, 1033
StyD4I CCNGG 2 cut(s) 126, 178
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 2 cut(s) 151, 523
TaqI TCGA 2 cut(s) 679, 751
TasI AATT 6 cut(s) 209, 477, 509, 663, 675, 788
TfiI GAWTC 5 cut(s) 138, 289, 326, 753, 824
Tru1I TTAA 1 cut(s) 1016
Tru9I TTAA 1 cut(s) 1016
TscAI CASTG 1 cut(s) 786
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 2 cut(s) 784, 952
Tsp45I GTSAC 1 cut(s) 366
TspDTI ATGAA 5 cut(s) 270, 583, 675, 750, 962
TspRI CASTG 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 700
VspI ATTAAT 1 cut(s) 1016
XapI RAATTY 3 cut(s) 209, 509, 675
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 2 cut(s) 549, 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.