pycom03g02210

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007872
Physical Location & Seq
Forward (+)
1687874 .. 1701396
13523 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02210.1

Sequence Viewer

Length: 10845 bp
ATGAGCTGGCAGCATCTTTCATCTCTGCTTGCTTGCACTGATGCGGATGTGGTTGAGGGTTGCCTGCAGACTTTGGCTGCTTTTGTAAAGAAAACAGTCGGAAAGTATTCCATTAGAGATGCTGCACTAAATTCAAAGTTATTCGCTCTTGCACAAGGTTGGGGGGGAAAAGAAGAGGGTCTTGGATTAATTGCTTGTGCAGTACAGAGTGGTTGTGACCCTGTTGCCTATGAGTTAGGTTCCACCCTTCATTTTGAGTTCTATGCATCCAATGATTCAACAGGTGACATCCCTGCTAACCAAGGATTACAAATCATTCACTTACCTAACATCAATACTCATCCAGAAACAGATCTTGAGCTTTTGCGTAAGTTAATTGCTGAGTACAAAGTACCCTCAAGCTTAAGATTTTCTTTATTGACAAGATTGCGGTTTGCAAGGGCTTTTGGTTCTGTGGCTACTCGGCAGCAGTATGCATGCATCCGCTTATATGCCTTCATCGTTCTTGTTCAAGCAAATAGTGATGCAGATGATCTTGTTTCTTTCTTCAACACTGAACCTGAGTTTATCAATGAATTAGTGTCATTGTTGAGCTTTGAGGATGTGGTTCCTGAGAAAATTCGAATTCTGTGTCTTCTTTCACTGGTTGCTCTTTGTCAAGATCGATCCCGGCAGCCTACTGTTTTGACTGCTGTCACATCTGGTGGTCATCGTGGAATCCTATCCAGTCTCATGCAGAAGGCCATTGATTTTGTCACCAAAGATACATCAAAGTGGTCTGTTGTTTTTGCTGAGGCTCTATTATCTCTTGTCACAGTTTTGGTGTCATCATCATCAGGTTGTTCAGCCATGCGTGAAGCTGGTTTTATCCCCACCCTTCTGCCTTTGCTTAAAGATACAAACCCACAACACCTTCATTTGGTCAGCACATCTGTGCACATTTTAGAAGCTTTTATGGATTACAGTAATCCTGCTGCTGCATTGTTTCGGGATTTGGGTGGTTTAGACGATACAATCTCTCGCCTACAGGTGGAAGTGTCTCATGTAGAAAATGGCTCAAAGCATCAAGATGAGGATTCTGATATCATTGGGAGTAGTGCACAAGTAGTTGCAGGAACTTCCACAGAGTTAGACAACATGCAGCCTTTGTATTCAGAACCACTGGTTTCATATCACAGACGACTGCTTATGAAAGCTTTATTGCGTGCTATATCCCTGGGGACTTATGCCCCTGGAAATACTGCCCGTGTTTATGGCTCAGAGGAGAGTCTATTGCCGCAATGCTTATGCATTATATTTAAAAGGGCAAAGGATTTTGGTGGCGGGGTATTCTCACTGGCGGCAACTGTCATGAGTGATTTAATACACAAAGATCGATCTACCTGTTTTCCAGTCTTAGATGCAGCAGGCCTTCCTTCTGCCTTCTTGGATGCTATAATGGATGGTGTTCTTTGCTCTGCGGAAGCCATTACATGCATACCACAATGTCTGGATGCCCTATGTTTAAATACTACTGGTCTTCAGGCCATGAAAGATCGCAATGCTTTGAGGTGTTTTGTGAAGATATTTACTTCGAGAACTTATCTGCGTGCCCTGACCAGTGACACACCAGGCTCGTTATCTAGTGGATTGGATGAATTAATGCGCCATGCTTCCTCATTGCGTGGGCCTGGAGTGGATATGCTGATTGAGATCTTAAATGCCATTTCAAAAATTGGACATGGGGTTGATGCTCCTTACATGTCAATTGATCCTTTGGGCTCTTCTACTCCTGTTCCCATGGAAACAGATGGAGAAGAGAGGAATTTGGTCCTGTCTGATAACGGGGAATCATCCAAAATGGAGAGTTCAGAGCAGATTGTGGAACCACCTTCTGATTCGTCAGTGGGGAATGTTGAATTATTTCTTCCTGATTGTGTGAGCAATGCTGCACGTCTTCTTGAAACAATACTTCAGAATGGTGACACATGTCGTATATTTGTGGAGAAGAAGGGGGTTGAAGCTGTCCTGCAGTTATTTACCTTGCCCTTGATGCCTCTTTCTGTTTCAGTTGGCCAGAGTATTTCTGTTGCATTTAAGAACTTCTCACCACAACATTCTGCTTCTTTGGCTCGGGCAGTTTGTTCATTCTTGAGAGAGCATATGAAATCAACAAATGAACTCTTAGTTTCAGTTGGAGGGACTCAACTTGCTTTGGTGGAATCTGCGAAGCAAACTCAAGTTTTGAGACATCTTTCCAGTCTTGAAGCTATTCTATCTCTGTCTAATGTTCTGATGAAAGGGACCACTACTGTTGTGTCTGAACTGGGTGCAGCAGATGCAGATGTATTGAAAGATCTCGGGAGCACATATAGGGAAATAATTTGGCAAATCTCTCTGTGCAATGATGTCAAGTTAGGGTTCAATGAGTTTAAGATCAATGCTGAACAAGAACCAGAGAGTGCTGAGGCAGGTCTGACTAATGCGTCTGGAAGGGAGAGTGATGATGATGCTAATATACCAATGGTGAGATATATGAACCCAGTTTCTATCAGGAATCAGCCCTTGTGGGGTGGAGAACGCGAGTTTCTTTCAGTTGTTCGCTCTGGTGAAGGTTTGCACCGTCGTAGTCGTCATGGGTTTACACGCATAAGGGGTGGAAGGACAAGCCGGCATTTGGAGGCTTTAAATGTTGATTCTGAATCTTCATCAACAATATCGGAGACATCTACTTCCCAGGATTTGAAAAAGAAAAGTCCTGATGTTCTTGTGATAGAAATCCTCAACAAGCTGGCTTCAACACTGCGTTCCTTCTTCACAGCTCTTGTCAAGGGATTCACATCACCAAATCGGCGTAGAGGTGACTCTGGGTCATTGAGTTTGGTTTCCAAGACTCTTGGAACTGCCCTAGCTAAAATATTTCTCGAGTCTCTTAGTTTCTCTGGGCATTCTACATCAGCTGGGCTTGATACTTCACTGTCAGTCAAGTGTCGATATCTTGGCAAAGTTGTCGATGATATGGTATCACTTACATTCGACAGCAGGCGGCGAACTTGTTATACTGCAACAGTAAATAATTTCTATGTGCATGGTACTTTTAAGGAGCTGCTTACAACATTTGAAGCTACTAGTCAGTTGTTGTGGACACTACCATACTCTGTGTCAGCATCAGGTATTGATCCTGAAAAGACAGGTGAAGGAAGTAAACTGTCCCACGGTTCATGGCTGCTTGATACTTTACAGAGCTATTGCCGTCTGCTCGAGTATTTTGTTAATTCTTCTTTACTCTTATCCACAACCTCTGCATCTCAGGCGCAGTTGCTTGTTCAGCCAGTTGCAGTTGGTTTGTCAATCGGGCTTTTCCCTGTGCCAAGGGACCCAGAAGTTTTTGTTCGTATGTTGCAATCTCAAGTTCTTGATGTGATACTTCCAGTCTGGAACTACCCTTTGTTTCCAAATTGTAGTCCAAGTTTCATTGCTTCTATTGTCTCGCTTGTCATGCATGTATATTCTGGTGTTGGAGATGTGAAGCAGAATCGCAGTGGCATTGCTGGAAATACAAACCAGCGATTGATACCCCCACCACTTGATGAAAATACTATTACCACCATTGTTGAGATGGGTTTTCCAAGAGCTAGAGCAGAGGAAGCTCTTAGGCGGGTGGAAACAAATAGCGTTGAAATGGCCATGGAGTGGCTCTTTAGTCATCCTGAGGATCCTGTGCAGGATGATGATGAACTGGCTCGGGCACTTGCTCTTTCACTAGGAAATTCATCAGATGCATCCAAAGCTGATAGTGTTGAAAAGTCTGTTGATGTGCTGGCAGAAGAGGGTTGTGTGAAAGCTCCTCCTGTTGATGATGTCCTTGCAGCGACGGTCAAATTGCTTCAAAGTAATGATACCATGGCATTCCCATTGACAGATTTGCTTGTGACGCTTAGCAACCGGAACAAAGGTGAAGACCGTCCAAGAGTTGTATCTTATCTTACTCAACAGCTAAAGAATTGTCCATTGGATTTTTCTAATGATACCAGTGCATTGAGTATGGTTTCACATGTTATTGCATTACTTCTTTCCGAGGATGGAAGCACAAGGGAAATCGCTGCAAGGTATGGTATTGTGTCTACTGCAACAGATATCTTGATGAACTTTAAGGGCAAAGATGAGTCAGGCAATGAGTTTCTTGTCCCAAAATGTATCAGTGCTCTTCTGCTTATTTTGGATAACATGTTGCAATCAAGGTCCAGAATTTCTGAAAAGGTTGAAGATACTCAAACGGGGCCTTTACCTGAATTATCTGGAGAGCTTGCGTCAATCCCTGCATCAGATACAGAGAAAAAACAGCCTATGGATGCCCATGAAAGAGATTCTGCCACGGCATTTGAGAAAATATTGGGGAAATCTACTGGTTATTTGACTATGGAAGAGAGTCATAAGGTGCTAGCAGTTGCTTGTGATTTGATAAAGCAGCATGTCCCAGCAATGATCATGCAGGCTGTTTTACAATTATGTGCTCGCTTGACAAAAACTCATGCTCTAGCATTAGAATTCCTTGAAAATGGAGGCCTGGCAGCTCTATTTGGCCTTCCAAGAAGTTGTTTTTTCCCTGGATATGATACTATTGCATCTGCTATCGTTCGCCATCTCCTTGAAGATCGACAAACACTGCAAACAGCAATGGAATTGGAGATTCGCCAAGCCCTGAGTGGAAACAGGCACGGTGGGCGCACTTCTGCACGAACATTCTTGACATCAATGGCACCTGTAATCTCCAGAGATCCAGTGGTTTTTATGAAAGCTGCTTCTGCAGTTTGCCAGTTGGAGACATCAGGAGGTAGGACCTTTGTTCTGTTATTGAAGGAAAAAGAGAAGGAAAAGGAAAAATCTAAAGCAGCAGGTGATGAGGCTGGATTATCTTCCAATGAGTGTGTGCGCATTTCTGAAAATAAGATACACGATGGATCTGGTAAATGCTCAAAAAGTCACAAGAAGATCCCTCCCAATCTCACTCAAGTGGTAGATCAGCTTCTTGAAATAGTCTTCAAATACCATTTCCCAAATAGCCAGGAAGACTCTGCGAATAACCCATCCGCAATGGAGGTAGATGAGCCCACTATGAAGGTGAAGGGTAAATCAAAGGTTGAAGAGATAAGGAAAGTGGAGTCTGAATCTGAAAGATCTGCAGGGTTGGCTAAAGTTACCTTTGTTCTCAAATTACTGAGTGATATTCTTCTTATGTATGTACATGCAGTTGGGGTTATACTTAAACGGGATTTAGAAATGACTCAATTGCGGGGATCTAATCAACCAGATGGTCTTGGACATGGTGGTATACTGCACCATGTAATTCATCGATTGCTTCCACTCACCATTGATAAATCTGCTGGACCCGATGAATGGAGAGACAAGTTGTCCGAAAAAGCTTCTTGGTTCCTGGTAGTTCTGTGTGGTCGTTCCAGTGAAGGGCGCAGACGAGTAATTATTGAACTTGTAAAGGCTTTATCCTTGGTTTCGAACTTGGATATTACTTCCACCTTATTACCTGATAAAAGGGTTTATGCGTTTGTTGATTTGGTGTATTCAATTCTGTCAAAAAATTCATCATCCAGCAACTTACCTGGTTCTGGATTTTCACCAGACATAGCAAAAAGCATGATAGATGGGGGAATGATTCAGTGTCTGACTGGTATCCTTCGGGTGATTGACTTAGACCATCCTGATGCTCCCAAAACTGTGAATTTGATACTCAAGGTGTTAGAAAGCCTAACAAGAGCTGCTAATGCTAGTGAGCAATACTTTAAATCTGATGAGACCAGCAAGAAAAAATCCACAGGGTTGAATGGAAGATCTGATGATCAGGTAACTGCCCCATCAGCTGATACAACTGTGGGGGATAATCAGAATGTAAGCAGTGAGCAGGGTGTGGGAGATATCGTACAGGTTGTTCAAGGGGATCAAGGAACTTCTCAAAGTGAAGCTAATCCTGATGGAAATCCAAACCAGTTGGTGGAACAAGATATGAGGATAGAAGTTGAAGGACCGCTAGCTTCTAATCCACCTATGGAGCTTGGGATGGATTTCATGCGTGAGGGGATGGATGAAGGTAATGTACTGCACAACATTGATCAAATTGAGATGACTTTTCGTGTTGAGAATAGGGCAGATGATGACATGGCTGATTTAGAAAATGACATGGGTGATGATGGCGAGGATGATGAGGATGATGACGAGGGAGAGGATGAGGATGAGGATATAGCAGAGGACGGTGGTGGCATGATGTCTCTCGCTGATACTGATGTGGAAGACCATGATGGTACCGGCTTGGGTGATGATTACAATGATGAAATGATTGATGAAGATGATGACGATTTTCATGAGAATCGTGTCATAGAAGTGAGATGGAGGGAAGCCCTTGATGGGCTTGACCATTTGCAGGTACTTGGACAACCGGGAGCTGCAAGTGGTCTCATTGATGTTGCTGCTGAACCTTTTGAAGGTGTTAATGTGGATGACTTGTTTGGTCTAAGAAGGCCGTTGGGTTTTGACCGCCGGCGTCAGACAAGTAGGTCTTCCTTTGAGCGATCTGTCACAGAAACAAATGGATTTCAACATCCTCTCCTTTTAAGACCATCCCAGTCTGGGGATTTGGTCTCAATGTGGTCAGCAGGTGGAAATTCATCCAGGGATTTAGAAGCCCTGTCATCAGGGAGCTTTGATGTAGCTCATTTCTACATGTTTGATGCTCCTGTTCTACCTTTTGATCATGTACCAGGCAATATTTTTGGTGACCGCTTGGGTGGTGCAGCACCCCCACCGTTGACTGATTATTCCGTAGGTATGGATTCATTGCAGTTATCGGGGCGAAGAGGACCAGGTGATGGTCGATGGACCGATGATGGCCAGCCTCAAGCAGGTCCTCAAGCCGCTGCTATTGCACAAGCAGTGGAGGAACAGTTTATATCACAGTTACGCAGTCTTGCTCCAGCTGATATCCCTGCTGAACGACAGTCCCAGAACTCAGGAGTGCAGGAGAAACAGCCAGATCTCCCTCCTTTAAGTGATAGTCAAGTAGCAGTGGAGCGTGATGACAGCCATGAAAGAAATGAAGATCAGCATCAAGTTGGGGTTGATGAAACAACACATCAAGTTAACTCAATTTCTGACGCTGCTCCCTGTCAAGAACAAGTCAATCCAGAATCTGTAGTTGAAGGTGCAGGGGAGTTCTTACAGGTACCTGAACCAATGTCAATTATGCCACCTTCAACCAGCAGTACACCAAGCGATAGCATGGACATTGGTGATGGGAATGGTGCTGCTGGTGAGCAAGTAGGGTCAATGCCAGGTTCTGTCAACTCGTCTGCAGAAATAAGTGCTGGTTTACAATGTGAAGGGTTTTCTGCTGTGCCTCCTAATGCCCATGATGTTACAGTGGAGGCTGTGGGTTGTGATAGATCCTCAAGAACAGAGGGGCAGGTTGGTAATGTGCCTACTCCAGATGAACCATCAAGGGAAAACACAACTGTTGCTCAAGAAGCTAATCTGGCTGAACAAGATTTGAATAATGAGGCTAATGGTGCTAACGCAATTGATCCAACCTTCTTGGAAGCTCTACCTGAAGATTTGAGGGCCGAAGTTCTTGCTTCACAGCAAGTTCAGCCCGTTCAACCTCCATCTTATGTGTCACCTTCTGCAGATGACATTGATCCTGAGTTTTTAGCAGCCCTTCCTCCAGATATCCAAGCAGAAGTTTTGGATCAACAAAGAGCACAACGGGTTGCACAGCAGGCTGAGGGACAACCGGTTGACATGGACAACGCTTCAATAATTGCTACTTTTCCAGCTGATTTACGTGAAGAGGTGCTTTTAACTTCTTCAGAAGCAGTTCTGTCTGGATTGCCTTCTCCATTGCTCGCTGAAGCCCAAATGCTAAGAGACCAAGCAATGAGTCATTATCAGGCTCGCAGCCTTTTTGGGACGAGCCACAGGCTAAATAACCGGAGAAATGGATTGGGTTTTGACCGGCAGACGGTGATGGACAGAGGTGTTGGAGTCCCTATTGGCCGGAGGGCAGTTTCTGCTCTTGCAGACAGCTTAAGGGTTAAGGAAATTGAAGGAGAGCCGCTCCTGGATGCTGATGCATTGAAAGCCTTAATTAGGCTATTACGATTGGCGCAGCCCCTTGGTAAAGGACTCTTGCAGAGGCTCTTGTTGAACTTATGTACCCACAGTGTTACACGAGCAATCTTAGTTCGTCTTTTGCTCGATATGATCGATCCCAAGGCTGAGGGCTTAATCGGTGGATTAGCAGCTATTAATTCCCAAAGGCTTTACGGTTGCAATTCAAATGTTGTTTATGATCGATCACAGTTGTTAGATGGTCTTCCTCCCTTGGTGCTGCGTCGAATTCTTGAGATCTTGACTTATTTGGCCACAAATCATTCTGCTGTGGCAAATATGTTATTCTTCTTTGATTTCTCTGGTGTTCCTGAGTCATTAAGCCCTATGCATGTGGAAACCAAGAAGGACAAAGGCAAGGAGAAAATTGGAGAAGCAGGATCTTCAAAAACTTCTGGAAACACCCAGGATGCTGACATTCCCCTGATATTGTTCCTGAAGCTCCTGAACCGACCTCATTTTGTACACAGCACTGCTCATCTTGAGCAGGTCATGGGCCTGCTTCAGGTTATTGTGTATACTTCAGCTTCAACATTAGAGGGCCAGTCTCAATCTGAGGGGGCAGATAAGCCTGTTGGTGAAGCATCAGGAGATGGTCAAAAGGGTCCTCCTTTGGAATCAGAATCTGGTCAGGGAGATAAGCCTGTTAGTGGTGAATCATCAACTTCAGATCGAAAGAGGAGTACGGATACATATAATGTTTTCTTGAAGCTGCCAGAATCTGATCTGCACAATCTTTGCAGCCTTATTGGTCGTGAGGGGCTTTCAGATAAACTTTATATGCTTGCTGGTGAGGTCTTGAAGAAGTTGGCCTCAGTTGCAGCGCCCCATCGAAAACTTTTTGTCTCAGCGCTGTCAGAACTAGCTCGTGGTTTGAGTGCCTCAGCTGTTGGGGAGCTTGTCACTCTCCGGAACACACATATGTTGGGTTTAAGTGCTGGATCAATGGCTGGGTCAGCAATCTTACGTGTATTACAAGCACTTTGCTCCCTCACTTCACCTAGGGCTAGTGAGAATTCAGGACTGGAGAATGATGCGGAGCAGGAGCATACCATCATGTGGAAGCTAAATGTTGCACTTGAGCCACTGTGGCAAGAATTAAGCAACTGTATAAGTGCGACTGAAACAGCTCTGGGTCAGAGTTCTTTCTGTCGAACCATGTCTATAGTAAATGTTGGAGACCATGCACAGGGTTCTTCATCTCCTCTCCCCCCTGGAACTCAGAGGCTCCTGCCTTTTATGGAGGCATTTTTTGTTTTATGTGAAAAGCTACAAGAGAACCACTCTACAATGCTGCAAGATCAGGCGAATATAACTGCAAGAGAAGTCAAAGAGTCCTCTGGAAACTCAGATCCTTCAACTACTAAGTGCCACAGTTATGGGGATTCTCAGAGAAAGCTTGATGGTGCTATTACATTTACAAAGTTCGCTGAGAAGCACCGCCGGCTTTTAAATGCTTTTATCAGACAGAATCCAGGTTTGTTGGAGAAATCCCTTACTATGATGCTGAAGGCACCAAGGCTAATTGATTTTGACAACAAGAGAGCGCATTTCCGGTCAAGAATTAGGCAACAGCATGAGCAACATCTCTCTGGTCCTCTGAGAATAAGTGTTCGGCGGGCTTATGTTTTGGAGGATTCTTATAATCAGTTGCGCATGCGGCCTACTCATGACATGAAGGGACGATTAAATGTCCAATTCCAGGGGGAAGAGGGTATTGATGCTGGAGGTCTGACACGAGAGTGGTATCAATTACTGTCGAGGGTCATATTTGACAAGGGGGCATTGCTTTTCACCACTGTAGGAAACAATGCAACTTTCCAGCCAAACCCTAATTCTGTCTACCAGACAGAACATCTGTCATATTTTAAATTTGTGGGCCGTGTGGTTGCAAAGGCACTTTTTGATGGGCAACTTTTGGATGTTTATTTCACTCGATCGATCTACAAGCATATACTTGGTGTAAAGGTGACTTACCATGATATAGAGGCTGTGGATCCTGATTACTACAAGAATTTAAAGTGGATGTTAGAGAATGACGTGAGTGACATTCCTGACCTGACGTTTAGCATGGATGCGGATGAAGAAAAGCATATCCTTTATGAGAAAAACCAGGTTACTGATTATGAGCTGAAACCTGGAGGAAGAAATATTCGGGTTACAGAAGAAACGAAGCACGAGTACGTTGACCTTGTAGCTGAACATATCTTGACAAATGCTATCCGTCCTCAAATTACTTCCTTCATGGATGGGTTTAAGGAATTGGTCCCGAGAGAGCTTATCTCTATTTTCAATGACAAGGAGCTTGAGCTACTCATCAGTGGTCTTCCAGAGATTGATTTGGCTGATTTGAAGGCCAACACCGAGTACACTGGCTACACATCAGCATCTGATGTTGTTAAATGGTTCTGGGACGTGGTTGAAAGTTTCGACAAGGAGGACATGGCGAGACTGCTGCAATTTGTGACTGGAACATCAAAGGTTCCATTGGAAGGTTTCAGGGCGTTGCAAGGTATATCTGGTGCTCAGAAATTTCAGATTCACAAGGCTTATGGAGCTCCTGACAGGCTGCCCTCCGCTCATACATGCTTTAACCAGCTAGACCTTCCCGAGTACACCACCAAAGAACAGCTTCATGAACGATTGTTACTTGCTATTCATGAAGCTAGTGAAGGATTTGGCTTTGGTTGA

Protein Analysis

3615

Amino Acids

394.34

Weight (kDa)

4.97

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 95 - 249 2.8e-25 Domain of Unknown Function (DUF908)
DUF913 PF06025 310 - 680 2.3e-83 Domain of Unknown Function (DUF913)
UBA_7 PF22562 1190 - 1237 6.7e-15 UBA-like domain
UBA PF00627 1190 - 1226 2.4e-07 UBA/TS-N domain
UBM PF14377 2518 - 2543 3.9e-08 Ubiquitin binding region
UBM PF14377 2556 - 2584 5.3e-09 Ubiquitin binding region
UBM PF14377 2591 - 2622 1.9e-07 Ubiquitin binding region
HECT PF00632 3303 - 3613 2.8e-95 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 9798
AarI CACCTGC 2 cut(s) 4865, 6635
AasI GACNNNNNNGTC 2 cut(s) 2464, 6544
Acc16I TGCGCA 2 cut(s) 4913, 9809
Acc36I ACCTGC 7 cut(s) 2441, 4865, 6403, 6635, 6881, 7441, 8632
Acc65I GGTACC 2 cut(s) 6293, 7210
AccB1I GGYRCC 4 cut(s) 4738, 6293, 7210, 9667
AccB7I CCANNNNNTGG 4 cut(s) 3672, 5986, 6857, 9213
AccBSI CCGCTC 2 cut(s) 8101, 10733
AccI GTMKAC 4 cut(s) 4103, 5311, 8672, 9996
AccII CGCG 1 cut(s) 2562
AccIII TCCGGA 1 cut(s) 9063
AcoI YGGCCR 5 cut(s) 2053, 3663, 6877, 8038, 8406
AcyI GRCGYC 1 cut(s) 6532
AfeI AGCGCT 1 cut(s) 9006
AflII CTTAAG 2 cut(s) 403, 8071
AflIII ACRYGT 6 cut(s) 1740, 1967, 4033, 4206, 6711, 9121
AgeI ACCGGT 1 cut(s) 7777
AhdI GACNNNNNGTC 2 cut(s) 2848, 5389
AhlI ACTAGT 1 cut(s) 3107
AjiI CACGTC 3 cut(s) 1934, 10195, 10570
AjuI GAANNNNNNNTTGG 6 cut(s) 165, 197, 3974, 4006, 9973, 10005
AleI CACNNNNGTG 3 cut(s) 932, 4991, 9895
Alw21I GWGCWC 8 cut(s) 939, 1102, 2348, 4186, 4495, 7747, 10681, 10714
Alw44I GTGCAC 2 cut(s) 935, 1098
AlwNI CAGNNNCTG 7 cut(s) 5659, 7178, 7325, 8054, 8780, 8876, 10544
Ama87I CYCGRG 7 cut(s) 2112, 2339, 2903, 3239, 3723, 10423, 10763
Aor13HI TCCGGA 1 cut(s) 9063
Aor51HI AGCGCT 1 cut(s) 9006
ApaLI GTGCAC 2 cut(s) 935, 1098
ArsI GACNNNNNNTTYG 2 cut(s) 616, 648
AseI ATTAAT 3 cut(s) 188, 1640, 8292
AsiGI ACCGGT 1 cut(s) 7777
Asp700I GAANNNNTTC 5 cut(s) 106, 1902, 2250, 7860, 10785
Asp718I GGTACC 2 cut(s) 6293, 7210
AspA2I CCTAGG 1 cut(s) 9155
AsuC2I CCSGG 2 cut(s) 670, 6429
AsuII TTCGAA 2 cut(s) 622, 5492
AsuNHI GCTAGC 2 cut(s) 4420, 6022
AvaI CYCGRG 7 cut(s) 2112, 2339, 2903, 3239, 3723, 10423, 10763
AvrII CCTAGG 1 cut(s) 9155
AxyI CCTNAGG 1 cut(s) 3690
BaeGI GKGCMC 4 cut(s) 939, 1102, 1594, 3730
BaeI ACNNNNGTAYC 6 cut(s) 5850, 5883, 6261, 6294, 8182, 8215
BalI TGGCCA 4 cut(s) 2055, 3665, 6879, 8408
BamHI GGATCC 2 cut(s) 3694, 10150
BanI GGYRCC 4 cut(s) 4738, 6293, 7210, 9667
BanII GRGCYC 3 cut(s) 1763, 5091, 10714
BarI GAAGNNNNNNTAC 6 cut(s) 4989, 5021, 6072, 6104, 10319, 10351
BauI CACGAG 4 cut(s) 8214, 9021, 9891, 10331
Bbv12I GWGCWC 8 cut(s) 939, 1102, 2348, 4186, 4495, 7747, 10681, 10714
BbvCI CCTCAGC 5 cut(s) 792, 2445, 7767, 8262, 9037
BceAI ACGGC 4 cut(s) 3216, 4371, 6496, 10020
BcgI CGANNNNNNTGC 6 cut(s) 2971, 3005, 7598, 7632, 10591, 10625
BciVI GTATCC 2 cut(s) 5678, 8836
BclI TGATCA 4 cut(s) 4464, 5833, 6103, 6739
BcnI CCSGG 2 cut(s) 670, 6429
BcuI ACTAGT 1 cut(s) 3107
BfoI RGCGCY 2 cut(s) 8981, 9008
BfrI CTTAAG 2 cut(s) 403, 8071
BfuAI ACCTGC 7 cut(s) 2441, 4865, 6403, 6635, 6881, 7441, 8632
BfuI GTATCC 2 cut(s) 5678, 8836
BglI GCCNNNNNGGC 1 cut(s) 9244
BglII AGATCT 7 cut(s) 352, 1692, 2335, 5156, 5825, 7021, 8391
BlnI CCTAGG 1 cut(s) 9155
BlpI GCTNAGC 1 cut(s) 3917
BmeRI GACNNNNNGTC 2 cut(s) 2848, 5389
BmeT110I CYCGRG 7 cut(s) 2112, 2339, 2903, 3239, 3723, 10423, 10763
BmgBI CACGTC 3 cut(s) 1934, 10195, 10570
BmrI ACTGGG 3 cut(s) 2315, 2516, 6607
BmtI GCTAGC 2 cut(s) 4424, 6026
BmuI ACTGGG 3 cut(s) 2315, 2516, 6607
BoxI GACNNNNGTC 2 cut(s) 692, 1968
BplI GAGNNNNNCTC 6 cut(s) 8085, 8117, 9422, 9454, 10421, 10453
BpmI CTGGAG 9 cut(s) 1692, 4299, 4735, 6945, 7455, 7692, 9200, 9900, 10314
Bpu10I CCTNAGC 5 cut(s) 792, 2445, 7767, 8262, 9037
Bpu1102I GCTNAGC 1 cut(s) 3917
Bpu14I TTCGAA 2 cut(s) 622, 5492
BpuMI CCSGG 2 cut(s) 670, 6429
Bsa29I ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
BsaAI YACGTR 2 cut(s) 7829, 9122
BsaBI GATNNNNATC 5 cut(s) 1691, 2756, 2818, 5970, 7092
BsaHI GRCGYC 1 cut(s) 6532
BsaI GGTCTC 5 cut(s) 5783, 6449, 6634, 7905, 9327
BsaWI WCCGGW 5 cut(s) 3924, 7777, 7974, 9063, 9708
BsaXI ACNNNNNCTCC 6 cut(s) 5898, 5928, 6579, 6609, 6917, 6947
Bse118I RCCGGY 6 cut(s) 2649, 6296, 6528, 7777, 7998, 9597
Bse21I CCTNAGG 1 cut(s) 3690
Bse8I GATNNNNATC 5 cut(s) 1691, 2756, 2818, 5970, 7092
BseAI TCCGGA 1 cut(s) 9063
BseCI ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
BseJI GATNNNNATC 5 cut(s) 1691, 2756, 2818, 5970, 7092
BseRI GAGGAG 4 cut(s) 1277, 3816, 8848, 9348
BseSI GKGCMC 4 cut(s) 939, 1102, 1594, 3730
BseYI CCCAGC 3 cut(s) 2939, 4456, 9104
Bsh1236I CGCG 1 cut(s) 2562
Bsh1285I CGRYCG 1 cut(s) 10094
BshNI GGYRCC 4 cut(s) 4738, 6293, 7210, 9667
BshTI ACCGGT 1 cut(s) 7777
BshVI ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
BsiEI CGRYCG 1 cut(s) 10094
BsiHKAI GWGCWC 8 cut(s) 939, 1102, 2348, 4186, 4495, 7747, 10681, 10714
BsiHKCI CYCGRG 7 cut(s) 2112, 2339, 2903, 3239, 3723, 10423, 10763
BsmI GAATGC 2 cut(s) 2926, 3887
Bso31I GGTCTC 5 cut(s) 5783, 6449, 6634, 7905, 9327
BsoBI CYCGRG 7 cut(s) 2112, 2339, 2903, 3239, 3723, 10423, 10763
Bsp119I TTCGAA 2 cut(s) 622, 5492
Bsp13I TCCGGA 1 cut(s) 9063
Bsp1407I TGTACA 2 cut(s) 5221, 8617
Bsp1720I GCTNAGC 1 cut(s) 3917
Bsp19I CCATGG 3 cut(s) 1779, 3666, 3882
BspDI ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
BspEI TCCGGA 1 cut(s) 9063
BspFNI CGCG 1 cut(s) 2562
BspHI TCATGA 5 cut(s) 1350, 6352, 9823, 10789, 10813
BspMAI CTGCAG 6 cut(s) 69, 2013, 4789, 5164, 7342, 7672
BspMI ACCTGC 7 cut(s) 2441, 4865, 6403, 6635, 6881, 7441, 8632
BspOI GCTAGC 2 cut(s) 4424, 6026
BspQI GCTCTTC 2 cut(s) 1768, 4191
BspT104I TTCGAA 2 cut(s) 622, 5492
BspT107I GGYRCC 4 cut(s) 4738, 6293, 7210, 9667
BspTI CTTAAG 2 cut(s) 403, 8071
BspTNI GGTCTC 5 cut(s) 5783, 6449, 6634, 7905, 9327
BsrBI CCGCTC 2 cut(s) 8101, 10733
BsrFI RCCGGY 6 cut(s) 2649, 6296, 6528, 7777, 7998, 9597
BsrGI TGTACA 2 cut(s) 5221, 8617
BssAI RCCGGY 6 cut(s) 2649, 6296, 6528, 7777, 7998, 9597
BssNAI GTATAC 2 cut(s) 5312, 8673
BssNI GRCGYC 1 cut(s) 6532
BssSI CACGAG 4 cut(s) 8214, 9021, 9891, 10331
Bst1107I GTATAC 2 cut(s) 5312, 8673
Bst2BI CACGAG 4 cut(s) 8214, 9021, 9891, 10331
BstACI GRCGYC 1 cut(s) 6532
BstAFI CTTAAG 2 cut(s) 403, 8071
BstAPI GCANNNNNTGC 2 cut(s) 2318, 8054
BstAUI TGTACA 2 cut(s) 5221, 8617
BstBAI YACGTR 2 cut(s) 7829, 9122
BstBI TTCGAA 2 cut(s) 622, 5492
BstDSI CCRYGG 5 cut(s) 1779, 3193, 3666, 3882, 4353
BstEII GGTNACC 1 cut(s) 6764
BstENI CCTNNNNNAGG 4 cut(s) 6471, 6888, 8131, 8657
BstFNI CGCG 1 cut(s) 2562
BstH2I RGCGCY 2 cut(s) 8981, 9008
BstMCI CGRYCG 1 cut(s) 10094
BstPAI GACNNNNGTC 2 cut(s) 692, 1968
BstPI GGTNACC 1 cut(s) 6764
BstSLI GKGCMC 4 cut(s) 939, 1102, 1594, 3730
BstUI CGCG 1 cut(s) 2562
BstXI CCANNNNNNTGG 1 cut(s) 9533
BstZ17I GTATAC 2 cut(s) 5312, 8673
Bsu15I ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
Bsu36I CCTNAGG 1 cut(s) 3690
BsuI GTATCC 2 cut(s) 5678, 8836
BsuTUI ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
BtgI CCRYGG 5 cut(s) 1779, 3193, 3666, 3882, 4353
BtrI CACGTC 3 cut(s) 1934, 10195, 10570
BtsI GCAGTG 7 cut(s) 2780, 3526, 4643, 5896, 6927, 7059, 8625
BveI ACCTGC 7 cut(s) 2441, 4865, 6403, 6635, 6881, 7441, 8632
CaiI CAGNNNCTG 7 cut(s) 5659, 7178, 7325, 8054, 8780, 8876, 10544
CciI TCATGA 5 cut(s) 1350, 6352, 9823, 10789, 10813
Cfr10I RCCGGY 6 cut(s) 2649, 6296, 6528, 7777, 7998, 9597
ClaI ATCGAT 6 cut(s) 664, 1375, 5332, 8250, 8338, 10094
CseI GACGC 6 cut(s) 2454, 3922, 4278, 6521, 7151, 8366
CsiI ACCWGGT 3 cut(s) 5596, 6850, 10266
CspAI ACCGGT 1 cut(s) 7777
CspCI CAANNNNNGTGG 6 cut(s) 2275, 2310, 3574, 3609, 4342, 4377
DraI TTTAAA 7 cut(s) 1300, 1506, 2667, 5779, 9606, 10024, 10173
DrdI GACNNNNNNGTC 2 cut(s) 2464, 6544
DriI GACNNNNNGTC 2 cut(s) 2848, 5389
DseDI GACNNNNNNGTC 2 cut(s) 2464, 6544
EaeI YGGCCR 5 cut(s) 2053, 3663, 6877, 8038, 8406
Eam1105I GACNNNNNGTC 2 cut(s) 2848, 5389
Ecl136II GAGCTC 1 cut(s) 10712
Eco147I AGGCCT 2 cut(s) 1410, 4545
Eco24I GRGCYC 3 cut(s) 1763, 5091, 10714
Eco31I GGTCTC 5 cut(s) 5783, 6449, 6634, 7905, 9327
Eco32I GATATC 6 cut(s) 1084, 2975, 4117, 5911, 6970, 7714
Eco47III AGCGCT 1 cut(s) 9006
Eco53kI GAGCTC 1 cut(s) 10712
Eco81I CCTNAGG 1 cut(s) 3690
Eco88I CYCGRG 7 cut(s) 2112, 2339, 2903, 3239, 3723, 10423, 10763
Eco91I GGTNACC 1 cut(s) 6764
EcoICRI GAGCTC 1 cut(s) 10712
EcoNI CCTNNNNNAGG 4 cut(s) 6471, 6888, 8131, 8657
EcoO109I RGGNCCY 5 cut(s) 3355, 4259, 4818, 6893, 8759
EcoO65I GGTNACC 1 cut(s) 6764
EcoRI GAATTC 4 cut(s) 624, 4526, 8382, 9169
EcoRV GATATC 6 cut(s) 1084, 2975, 4117, 5911, 6970, 7714
EcoT22I ATGCAT 9 cut(s) 268, 478, 482, 1292, 1478, 3483, 3763, 8119, 8487
EcoT38I GRGCYC 3 cut(s) 1763, 5091, 10714
FauI CCCGC 4 cut(s) 1316, 3630, 5265, 9765
FauNDI CATATG 2 cut(s) 2142, 9075
FbaI TGATCA 4 cut(s) 4464, 5833, 6103, 6739
FblI GTMKAC 4 cut(s) 4103, 5311, 8672, 9996
FriOI GRGCYC 3 cut(s) 1763, 5091, 10714
FspAI RTGCGCAY 1 cut(s) 4913
FspI TGCGCA 2 cut(s) 4913, 9809
GsaI CCCAGC 3 cut(s) 2943, 4460, 9108
GsuI CTGGAG 9 cut(s) 1692, 4299, 4735, 6945, 7455, 7692, 9200, 9900, 10314
HaeII RGCGCY 2 cut(s) 8981, 9008
HgaI GACGC 6 cut(s) 2454, 3922, 4278, 6521, 7151, 8366
Hin1I GRCGYC 1 cut(s) 6532
HincII GTYRAC 5 cut(s) 6798, 7129, 7330, 7783, 10342
HindII GTYRAC 5 cut(s) 6798, 7129, 7330, 7783, 10342
HindIII AAGCTT 5 cut(s) 400, 948, 1194, 5400, 9551
HpaI GTTAAC 1 cut(s) 7129
Hpy99I CGWCG 3 cut(s) 2607, 3856, 8382
HpyCH4IV ACGT 7 cut(s) 1933, 7828, 9121, 10194, 10217, 10338, 10569
HpySE526I ACGT 7 cut(s) 1933, 7828, 9121, 10194, 10217, 10338, 10569
Hsp92I GRCGYC 1 cut(s) 6532
KflI GGGWCCC 1 cut(s) 3355
Kpn2I TCCGGA 1 cut(s) 9063
KpnI GGTACC 2 cut(s) 6297, 7214
KroI GCCGGC 3 cut(s) 2649, 6528, 9597
KroNI GCCGGC 3 cut(s) 2651, 6530, 9599
Ksp22I TGATCA 4 cut(s) 4464, 5833, 6103, 6739
KspAI GTTAAC 1 cut(s) 7129
LguI GCTCTTC 2 cut(s) 1768, 4191
MabI ACCWGGT 3 cut(s) 5596, 6850, 10266
MaeII ACGT 7 cut(s) 1933, 7828, 9121, 10194, 10217, 10338, 10569
MbiI CCGCTC 2 cut(s) 8101, 10733
MfeI CAATTG 3 cut(s) 1746, 5267, 7563
MlsI TGGCCA 4 cut(s) 2055, 3665, 6879, 8408
MluNI TGGCCA 4 cut(s) 2055, 3665, 6879, 8408
MmeI TCCRAC 8 cut(s) 79, 2155, 3478, 4779, 7595, 8005, 9310, 9618
Mox20I TGGCCA 4 cut(s) 2055, 3665, 6879, 8408
Mph1103I ATGCAT 9 cut(s) 268, 478, 482, 1292, 1478, 3483, 3763, 8119, 8487
MreI CGCCGGCG 1 cut(s) 6528
MroI TCCGGA 1 cut(s) 9063
MroNI GCCGGC 3 cut(s) 2649, 6528, 9597
MroXI GAANNNNTTC 5 cut(s) 106, 1902, 2250, 7860, 10785
MscI TGGCCA 4 cut(s) 2055, 3665, 6879, 8408
MslI CAYNNNNRTG 9 cut(s) 772, 932, 1068, 4991, 5697, 6371, 9074, 9531, 9895
Msp20I TGGCCA 4 cut(s) 2055, 3665, 6879, 8408
MspA1I CMGCKG 6 cut(s) 2939, 5855, 6905, 6965, 7820, 9041
MspCI CTTAAG 2 cut(s) 403, 8071
MunI CAATTG 3 cut(s) 1746, 5267, 7563
Mva1269I GAATGC 2 cut(s) 2926, 3887
MvnI CGCG 1 cut(s) 2562
NaeI GCCGGC 3 cut(s) 2651, 6530, 9599
NciI CCSGG 2 cut(s) 670, 6429
NcoI CCATGG 3 cut(s) 1779, 3666, 3882
NdeI CATATG 2 cut(s) 2142, 9075
NgoMIV GCCGGC 3 cut(s) 2649, 6528, 9597
NheI GCTAGC 2 cut(s) 4420, 6022
NmeAIII GCCGAG 1 cut(s) 442
NsbI TGCGCA 2 cut(s) 4913, 9809
NsiI ATGCAT 9 cut(s) 268, 478, 482, 1292, 1478, 3483, 3763, 8119, 8487
NspV TTCGAA 2 cut(s) 622, 5492
OliI CACNNNNGTG 3 cut(s) 932, 4991, 9895
PaeI GCATGC 2 cut(s) 480, 9814
PaeR7I CTCGAG 2 cut(s) 2903, 3239
PagI TCATGA 5 cut(s) 1350, 6352, 9823, 10789, 10813
PaqCI CACCTGC 2 cut(s) 4865, 6635
PasI CCCWGGG 1 cut(s) 1216
PceI AGGCCT 2 cut(s) 1410, 4545
PciI ACATGT 5 cut(s) 1740, 1967, 4033, 4206, 6711
PciSI GCTCTTC 2 cut(s) 1768, 4191
PcsI WCGNNNNNNNCGW 1 cut(s) 8247
PctI GAATGC 2 cut(s) 2926, 3887
PdiI GCCGGC 3 cut(s) 2651, 6530, 9599
PdmI GAANNNNTTC 5 cut(s) 106, 1902, 2250, 7860, 10785
PflMI CCANNNNNTGG 4 cut(s) 3672, 5986, 6857, 9213
PfoI TCCNGGA 1 cut(s) 8103
PinAI ACCGGT 1 cut(s) 7777
Ple19I CGATCG 1 cut(s) 10094
Ppu21I YACGTR 2 cut(s) 7829, 9122
PpuMI RGGWCCY 4 cut(s) 3355, 4818, 6893, 8759
PscI ACATGT 5 cut(s) 1740, 1967, 4033, 4206, 6711
PshAI GACNNNNGTC 2 cut(s) 692, 1968
PshBI ATTAAT 3 cut(s) 188, 1640, 8292
PsiI TTATAA 1 cut(s) 9798
Psp124BI GAGCTC 1 cut(s) 10714
Psp5II RGGWCCY 4 cut(s) 3355, 4818, 6893, 8759
PspEI GGTNACC 1 cut(s) 6764
PspFI CCCAGC 3 cut(s) 2939, 4456, 9104
PspPPI RGGWCCY 4 cut(s) 3355, 4818, 6893, 8759
PspXI VCTCGAGB 1 cut(s) 3239
PsrI GAACNNNNNNTAC 6 cut(s) 3022, 3054, 5907, 5939, 10785, 10817
PstI CTGCAG 6 cut(s) 69, 2013, 4789, 5164, 7342, 7672
PstNI CAGNNNCTG 7 cut(s) 5659, 7178, 7325, 8054, 8780, 8876, 10544
PvuI CGATCG 1 cut(s) 10094
PvuII CAGCTG 5 cut(s) 2939, 5855, 6965, 7820, 9041
RseI CAYNNNNRTG 9 cut(s) 772, 932, 1068, 4991, 5697, 6371, 9074, 9531, 9895
SacI GAGCTC 1 cut(s) 10714
SapI GCTCTTC 2 cut(s) 1768, 4191
SexAI ACCWGGT 3 cut(s) 5596, 6850, 10266
Sfr274I CTCGAG 2 cut(s) 2903, 3239
SfuI TTCGAA 2 cut(s) 622, 5492
SgrAI CRCCGGYG 1 cut(s) 6528
SlaI CTCGAG 2 cut(s) 2903, 3239
SmiMI CAYNNNNRTG 9 cut(s) 772, 932, 1068, 4991, 5697, 6371, 9074, 9531, 9895
SpeI ACTAGT 1 cut(s) 3107
SphI GCATGC 2 cut(s) 480, 9814
SseBI AGGCCT 2 cut(s) 1410, 4545
SspI AATATT 4 cut(s) 2898, 4371, 6757, 10306
SstI GAGCTC 1 cut(s) 10714
StuI AGGCCT 2 cut(s) 1410, 4545
TaiI ACGT 7 cut(s) 1936, 7831, 9124, 10197, 10220, 10341, 10572
TaqII GACCGA 1 cut(s) 6884
TatI WGTACW 8 cut(s) 202, 384, 5221, 6088, 7250, 8617, 10521, 10767
TauI GCSGC 6 cut(s) 1279, 1343, 3028, 6905, 8101, 9817
TspGWI ACGGA 3 cut(s) 6799, 8855, 10367
Van91I CCANNNNNTGG 4 cut(s) 3672, 5986, 6857, 9213
Vha464I CTTAAG 2 cut(s) 403, 8071
VneI GTGCAC 2 cut(s) 935, 1098
VspI ATTAAT 3 cut(s) 188, 1640, 8292
XagI CCTNNNNNAGG 4 cut(s) 6471, 6888, 8131, 8657
XcmI CCANNNNNNNNNTGG 3 cut(s) 3595, 4682, 4759
XhoI CTCGAG 2 cut(s) 2903, 3239
XmaJI CCTAGG 1 cut(s) 9155
XmiI GTMKAC 4 cut(s) 4103, 5311, 8672, 9996
XmnI GAANNNNTTC 5 cut(s) 106, 1902, 2250, 7860, 10785
Zsp2I ATGCAT 9 cut(s) 268, 478, 482, 1292, 1478, 3483, 3763, 8119, 8487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.