Rroxscaffold_1G00005140

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007872
Physical Location & Seq
Forward (+)
6921160 .. 6937531
16372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005140.1

Sequence Viewer

Length: 7905 bp
ATGATGGCACTTACATTTGATAGCAGACGACGTACTTGTTATACTGCAACAATTAATAATTTCTATGTCCATGGTACCTTTAAGGAGCTGCTCACAACGTTTGAAGCTACTAGTCAGTTGTTATGGACCCTTCCATATTGTATGCCAACATCAGGCATTGATCATGAAAAGACAGGTGAAGGCAGTAAACTGTCCCATAGTTCGTGGCTGCTTGAGACTCTACAAAGCTATTGCCGTGTGCTGGAGTATTTTGTTAATTCTTCTTTACTCTTATCTACAACCTCTGCATCCCAGGCACAGTTGCTTGTTCAGCCAGTTGCAGTTGGTCTATCAATTGGGCTTTTCCCTGTGCCAAGGGAACCAGAAGTTTTTGTTCGTATGCTTCAGTCTCAAGTTCTTGATGTAATACTTCCTATCTGGAACCACCCCATGTTTCCAAATTGTAGTCCAGGATTCGTTGCTTCCATTGTTTCACTTGTTATGCATGTATATTCTGGTGTGGGAGATGTGAAGCAGAATCGCAGTGGCATTGCTGGAACTGCAAACCAAAGATTCATGCCTCCACCACTTGATGAAGGTACTATTACTACCATTATGGAGATGGGTTTTTCAAGGGCCAGAGCAGAAGAAGCGCTTAGGAGGGTGGAAACAAATAGCGTTGAAATAGCCATGGAGTGGCTTTGCAGCCATGCTGAGGATCCTGTGCAGGAGGATGATGAACTGGCCCGGGCACTTGCTCTATCACTGGGAAATTCATCTGAAACATCTAAAGCTGATAATGTTGACAAGTCTGTAGATGTGCTGGCAGAAGAGAGTTGTGTGAAAGCTCCTCCAGTTGATGATATTCTTGCCGCATCAGTTAAGTTGTTTCAGAGCAGTGACACAATGGCCTTCCCATTGACAGATTTGCTTGTGACACTTAGCAATCGGAACAAAGGTGAAGACCGTCCTAAAGTTGCATCTTATCTAATTCAGCAGCTCAAGCTTTGTCCATTGGATTTTTCTAAGGATACCAGTTCTTTGAGTATGTTATCACATGTTATAGCATTGCTGCTTTCTGAGGATGGAAGTACAAGGGAAATTGCTGCACAGAATGGTATTGTGTCTGCTGCAGTAGATATCTTAATGAATTTTAAGGCCAAAGACGAGTCAGGCAATGAACTTCTTGTTCCAAAATGTATTAGTGCTCTACTGCTTATTTTGGATAACATGTTGCAATCAAGGCCCAGAATTTCTGAGAACATTGAAGAGACTCAGACAGGATCTCTGTCTGAACTATCTGGAGAGCATACTTCCTTGTCAATCCCTGATGCAGTTACAGAGAAAAAACAGGATACGGATTCCCAGGAAAAAGATAGTGGCACTGGATTTGAGAAAATATTGGGCAAATCAACTGGTTATTTGACTATGGAAGAGAGTCAAAAGCTGCTGGCCGTTGCCTGTGATTTGATAAAGCAGCATGTCCCAGCTATGATCATGCAGGCTGTTTTACAGCTCTGTGCTCGTTTGACGAAAACTCATGCTCTTGCTTTGCAGTTTCTTGAACATGGAGGATTAACAGCTCTTTTTGGCCTTCCCAGAAGTTGTTTTTTCCCTGGATATGACACTGTTGCATCTGCTATTGTTCGCCATCTCCTTGAAGATCCGCAAACACTGCAAACAGCAATGGAATTGGAAATTCGCCAAACACTGAGTGGCAACAGGCATGGTGCACGTACTTCTGCAAGGACATTTTTGACATCAATGGCACCTGTAATCTCTAGAGATCCTGTTGTTTTTATGAAAGCTGCTGCTGCTGTTTGTCAGTTGGAGACATCAGCAGGTAGGACATTCATTGTCTTAATGAAGGAAAAAGAAAAGGAAAAGGACAAACCTAAAGCATCTGGTGGTGAGGTTGGATTATCTTCCAATGAATCTATCCGTATTCCTGAAAATAAAGTACATGATGGATCAGGGAAATGCTTGAAAGGTCACAAGAAGATCCCTGCTAATCTTACTCAAGTGATTGATCAGCTGCTTGAAATTGTTTTGAAATACTATTTTCCAAAGAGCCAGGAAGATTGTCAGAATAACCTGTCTTCTATGGAGGTAGATGAACCTGCTACCAAGGTAAAGGGTAAATCAAAGGTTGATGAGACAAGGAAAGTGGAGTCTGAATCTGAAAGATCTGCAGGGCTGGCAAAGGTAACATTTGTTCTCAAGTTACTGAGTGATATTCTTCTCATGTATGTACATGCAGTTGGTGTTATACTTAAACGGGATTTGGAATTGGCTCAATTACGGGTAGCTAATCAACTGGAAGGTCCTGGACATGGTGGTATACTTCATCATGTGATTCATCGATTGCTTCCACTCACCATTGATAAATCTGCTGGACCTGATGAATGGAGAGACAAGTTATCTGAAAAAGCTTCTTGGTTCCTGGTAGTTCTGTGTGGTCGTTCTGGTGAAGGGCGTAGAAGAGTAATTAGTGAGCTTGTGAAAGCATTATCATCATTTTCAAACATTGATGGTAATTCTTCGAAGAGCATCTTATTGCCGGATAAAAAGGTTTATGCTTTTGTGGATTTGGTGTACTCTATTTTGTCTAAAAATTCATCATCAAGCAACTTACCTGGTACGGGGTTTTCACCTGACATAGCAAAAAGCATGATAGATGGGGGAATGATTCAGTGTCTGACTAGTATTCTCCAGGTGATTGATTTGGACCATCCTGATGCTCCCAAAACTGTGAATCTCATACTCAAGGCTTTAGAAAGTCTAACCAGGGCTGCTAATGCTAGTGAGCAATATTTTAAATCTGATGAGACAAACAAGAAAAAGTCCATGGTGTTAAATAGAAGATCTGATGATCAGGTAACTACTCCAGCTGATGATACATTGGGCCATAATCAGAATACAAGCAGTGAGCAGGATGTCAGAGATGCAGTACCAACTGAACAACAGGATCAAGGAACTTCTCAAAGTGAAGGTAATCCTGATGCAACTCCGAACCAGTCAGGGGAACATGATATGAGAATTGAAGTTGAAGGACCATTGGCTTCTAACCAACCCATGGAGCTTGGGATGGATTTTATGCGTGAAGAGATGGAAGAAGGCAATGTGTTACACAACAATGACCAAATTGAAATGACTTTTCGTGTTGAGGGTAGGGCAGATGATGACATGGGTGATGAAGATGATGACATGGGTGATGATGGTGAGGATGATGAGGATGATGATGATGGAGAGGATGAGGATGAGGATATAGCAGAAGATGGTGGCGGCATGATGTCTCTGGCTGATACTGATGTGGAAGACCATGATGATACTGGCCTGGGAGATGATTATAATGATGAGATGATTGATGAAGATGATGATGATTTCCATGAGAATCGGGTCATAGAAGTGAGATGGAGGGAAGCCCTTGACGGGCTTGATCATTTACACGTACTTGGGCAACCTGGAGCTGCAAGTGGTCTGATTGATGTTGCTGCCGAACCTTTTGAAGGGGTTAATGTGGATGACCTTTTTGGTCTACGAAGGCCGTTAGGTTTTGATCGACGTCGTCAGACGAGTAGGTCTTCCTTTGAGCGATCTGTTACAGAAGCAAATGGCTTTCAACATCCTCTCCTTATAAGGCCTCCCCAGTCTGGAGATTTGGTATCCATGTGGTCAGCAGGTGGAAATTCATCCCGGGACTTAGAAGCTCTATCATCAGGGAGCTTTGATGTAGCTCATTTCTACATGTTTGATGCTCCTGTTCTTCCATATGATCATGTTCCAAGCAATCTTTTCGGTGACCGTTTGGGTGGTGCAGCCCCGCCACCATTGACTGATTATTCTGTAGGTATGGATTCATTGCAGTTATCGGGACGAAGAGGCCCAGGTGATGGCCGGTGGACTGATGATGGCCAGCCTCAAGCAGGTGCTCATGCTGCTGCTATTGCTCAGGCAGTTGAGGAACAGTTTATATCTCAATTGCGCAGTCTTGCTCCTGTTGACACCCCTGTCGAACCACATTCTCAGAATTCAGGAGTGCAGGAGAAACAGCCAGATGTGCCTCCTTCAACTGATGGTCAAGTAGTAGTAGATCGTGGTAACACCACCAGCCAACAAGTTGAAGATCAGCACCAAGACAGGGGTGTTGAGGTGACACATCAAGATATCTCCAGGCCTGAGGGTATTCCTCGCCAAGAACAAGTCAATCAAGAATCCTTTGTCGAAGATGCAGGAGGCTGTTTGCAGGAACCTGAACCAATGTCAATCCGGGCACATTCTTTGGACAGTACACGAAATGATAACATGGACATTGGGGAGGGGAATGGCGCTGCTGAGCAAGAAGGGTCAATGCCGGAATTTGTCAACTCATCTGCAAGCACAAGGGTTGATTTACAACAGGAAGAGGGTTCTGAAGTGCCTTCTAATGTCAATGATGCTACAGTAGAAGCTATGGGTCAGGATGGATCTTCAGGCAATCAAGTTGGTGACATGCCAGCAAATTTCGGCTTCAATGTGTCTAATTCAGATGAGCCGTCAAGCCAAAACACATTGGTTGCTGCAGAAGCTAATCAGGCTGAACAAGTTACTTTGAACAATGAGGCTCCTGGTGCTAATGCAATTGACCCTACATTCTTGGAGGCTTTACCTGAAGAGTTGAGAGCGGAGGTTCTAGCTTCACAGCAAGCTCAGTCAGTTCAACCTCCTCCTTATGCGCCACCTTCTGCGGACGACATTGATCCTGAGTTTTTAGCCGCCCTTCCTCCGGATATTCAAGCAGAAGTTTTGGCTCAACAAAGAGCACAAAGAGTTGCACAACAGGCTGAAGGACAACCTGTTGACATGGACAATGCTTCAATAATTGCTACTTTTCCTGCTGATTTACGGGAAGAGGTACTTTTAACTTCTTCAGAAGCAGTTTTGTCTGCATTGCCTTCTCCACTACTGGCTGAAGCTCAAATGCTAAGAGATCGAGCAATGAGTCATTATCAGGCTCGCAGCCTTTTCGGGAGCAGCCACAGGCTGAATAATAGGAGAAATGGATTGGGTTTTGACCGGCATACAGTAATGGACAGGGGTGTTGGAGTCACAATTGGCCGGAGGGCAGTTTCTTCTATTACAGATAGCTTAAAGGTCAAGGAAATTGAAGGCGAGCCACTTCTAGATGCAAATTCATTGAAAGCCTTAATTAGGCTTCTACGATTAGCACAGCCCCTTGGTAAAGGACTCTTGCAGAGGCTCTTCTTAATCCTTTGCACACACAGTGTTACAAGAGCAATTTTAGTGCGTCTTTTGCTCAATATGATCAAGCCTGAGGCTGAAGGCTCAGTCAGTGGATTAGCTACTATTAATTCCCAAAGGCTTTATGGTTGCCACTCAAATGTTGTATATGGCAGATCTCAGTTGTTAGATGGTCTTCCTCCCCTGGTGTTGCGTCGGATCCTTGAGATCTTGACTTATTTAGCTACAAATCATTCTGCAGTGGCAAATATGTTATTCTACTTTGATTTCTCAGGTGTTCCTCAGTCTTTAAGTCCCTTAAATATGGATACCAAGAAGGACAAAGGCAAAGAGAAAATCGGAGAAGGAGGATTTTCATCTAATCCTGTCAACACCCAGGATGGGGATGTTCCCCTGATATTGTTTCTGAAGCTCCTTAATCGACCTCATTTCTTGCGTAGCACTGCTCATCTTGAGCAGGTCATGGACCTGCTTCAGGTTGTTGTTGATAATTCAGCTTCAAAATTAGAGGTCCATTCTCCCTCTGAAAGGGTCGATGGGAATTCCCAAAATCTATCTATAAGTGAAACTTCTGGAGATGGTCAGAACGGTCGTCCTGTGGAACCAGAATCTCAGCAAGAGGTTAAACCTGATGGTGTTGGATCATCAACTTCAGATGCAAACAAAAGTACAGATACCCATAGTATTTTCTTGAAGCTGCCAGAATCCGATTTACATAATCTTTGCAGTCTTCTTGGTCGTGAGGGGCTTTCAGATAAAGTTTATATGCTTTCCAGTGAGGTGCTGAAGAAGTTGGCCTCAGTTGCAGTGACCCATCGAAAGTTCTTTATCTCGGAGCTTTCAGAATTAGCTCATGGTTTGAGTGCCTCAGCTGTCAGCGAGCTTGTCACTCTCCGGAATACACAGATGTTGGGTTTGAGTGCTGGTTCTATGGCTGGGTCGGCAATCTTACGAGTATTGCAATCACTTTGCTCACTCACTTCACCTGGTACAAATGAGAATTCAGTTTTGGAGAATGATGCAGAGCAGGAGGAGCATGCCACCATGTGGAAGCTAAATATTGCACTTGAGCCATTGTGGCAAGAATTGAGTGACTGTATAAGTGCCACTGAAACACAGTTGGGCCAGAGCTCTTTTTGTCCAACCATGTCCACTATAAATATTGGGGACCATGTACAAGGTTCCTCTTCTTCATCTCCTCTTCCTCCTGGAACCCAGAGACTGCTTCCTTTTATGGAGGCATTTTTTGTATTGTGTCAAAAGCTACAAGCAAACCACTCTATTACGCTGCAAGATCAAGCTAATGTGACTGCAAGAGAAGTCAAAGAGTCTGCTGGAAATTCAGATCCTTCAGGTACTAAGTTTTATAGCTGTGGGGATTCTCAGAGGAAGCTTGATGGTGCTGTCACATTTACAAGGTTTGCGGAGAAGCATCGCCGTCTTTTGAATGCATTTATCAGACAGAATCCAGGGCTGTTGGAGAAATCCCTTACTATGATGTTGAAGGCACCACGGCTAATTGATTTTGATAACAAGAGAGCATATTTCCGGTCAAGAATTAGGCAACAGCATGAGCAACATCTCTCCGGTCCGCTGCGAATAAGTGTTCGTCGTGCTTATGTTTTGGAAGATTCTTACAATCAGTTGAGAATGCGTCCTAATCAGGACATGAAGGGACGATTAAATGTCCAATTCCAAGGGGAAGAGGGTATTGATGCTGGTGGTCTAACGCGAGAATGGTATCAATTACTATCAAGGGTTATATTTGACAAAGGTGCATTGCTTTTCACTACTGTCGGAAACAATGCAACTTTCCAGCCAAACCCTAATTCTGTATACCAGACGGAACATCTTTCGTACTTTAAATTTGTGGGCCGCGTGGTTGCAAAGGCAGTATTTGATGGGCAACTTTTGGATGTTTATTTCACTCGATCCTTCTACAAGCACATACTTGGTGTAAAGGTGACATACCATGATATAGAAGCCGTGGATCCTGATTACTACAAGAATTTGAAATGGATGTTAGAGAATGATGTGAGTGATATTCCTGACCTGACGTTTAGCATGGATGCGGATGAAGAAAAGCATATTCTTTATGAGAAAAACCAGGTGACTGATTATGAGCTTAAACCTGGAGGACGAAATATCAGGGTTACGGAAGAAACTAAGCACGAGTATGTAGACCTTGTAGCTGATCATATTTTGACAAATGCTATTCGTCCACAAATTAATTCTTTCTTGGAGGGGTTCAATGAATTGGTTCCACGCGAGCTTATCTCGATTTTTAATGATAAAGAGCTTGAGCTACTTATCAGTGGTCTTCCGGAGATTGATTTGGATGACTTGAAGGCAAACACCGAGTACACTGGCTATACAGCAGCATCTAGTGTTGTACAATGGTTTTGGGAGGTGGTTAAATGTTTCAACAAGGAGGACATGGCAAGACTGCTTCAGTTTGTAACTGGCACATCAAAGGTTCCATTGGAAGGTTTCAAGGCTTTGCAAGGCATCTCTGGTCCTCAGAGGTTTCAGATTCACAAGGCATATGGAGCTCCTGACAGGCTGCCCTCTGCTCATACATGCTTTAATCAACTAGACCTTCCTGAGTATACCTCTAAGGATCAGCTTCATGAACGGTTGATGCTTGCTATTCATGAAGGGAGTGAAGGATTTGGCTTTGGCTGA

Protein Analysis

2634

Amino Acids

289.5

Weight (kDa)

4.85

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_7 PF22562 191 - 235 1.3e-13 UBA-like domain
UBA PF00627 194 - 227 2.1e-06 UBA/TS-N domain
UBM PF14377 1530 - 1557 1.4e-08 Ubiquitin binding region
UBM PF14377 1567 - 1596 1.1e-10 Ubiquitin binding region
UBM PF14377 1603 - 1634 1.4e-07 Ubiquitin binding region
HECT PF00632 2323 - 2633 4.1e-96 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3330, 3617
AarI CACCTGC 2 cut(s) 3650, 3896
AasI GACNNNNNNGTC 2 cut(s) 3559, 3989
AatII GACGTC 1 cut(s) 3547
Acc16I TGCGCA 1 cut(s) 3965
Acc36I ACCTGC 6 cut(s) 1811, 2107, 3650, 3896, 5668, 5697
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 3 cut(s) 74, 1747, 6727
AccB7I CCANNNNNTGG 4 cut(s) 675, 3055, 3872, 6267
AccBSI CCGCTC 1 cut(s) 4643
AccI GTMKAC 5 cut(s) 2320, 3517, 7056, 7401, 7829
AccII CGCG 3 cut(s) 6952, 7098, 7488
AccIII TCCGGA 3 cut(s) 4744, 6114, 7543
AclI AACGTT 1 cut(s) 98
AcoI YGGCCR 4 cut(s) 1431, 3874, 3892, 5074
AcyI GRCGYC 1 cut(s) 3544
AdeI CACNNNGTG 3 cut(s) 1694, 5243, 6267
AfeI AGCGCT 1 cut(s) 633
AflIII ACRYGT 4 cut(s) 1036, 1209, 3427, 3726
AhlI ACTAGT 2 cut(s) 110, 2681
AjuI GAANNNNNNNTTGG 4 cut(s) 6212, 6244, 7033, 7065
Alw21I GWGCWC 7 cut(s) 1189, 1504, 1714, 3913, 4783, 6353, 7774
Alw26I GTCTC 9 cut(s) 209, 393, 1244, 1805, 2129, 2385, 2801, 3279, 6433
Alw44I GTGCAC 1 cut(s) 1710
AlwNI CAGNNNCTG 2 cut(s) 2677, 6442
Ama87I CYCGRG 2 cut(s) 726, 3675
Aor13HI TCCGGA 3 cut(s) 4744, 6114, 7543
Aor51HI AGCGCT 1 cut(s) 633
ApaLI GTGCAC 1 cut(s) 1710
AseI ATTAAT 3 cut(s) 54, 5328, 7449
Asp700I GAANNNNTTC 1 cut(s) 7479
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 4 cut(s) 634, 3966, 4310, 4696
AsuC2I CCSGG 5 cut(s) 727, 728, 3676, 3677, 4250
AsuII TTCGAA 1 cut(s) 2522
AvaI CYCGRG 2 cut(s) 726, 3675
AxyI CCTNAGG 2 cut(s) 4158, 5292
BaeGI GKGCMC 3 cut(s) 733, 1714, 4255
BaeI ACNNNNGTAYC 2 cut(s) 3276, 3309
BalI TGGCCA 1 cut(s) 3894
BamHI GGATCC 3 cut(s) 697, 5418, 7210
BanI GGYRCC 3 cut(s) 74, 1747, 6727
BanII GRGCYC 2 cut(s) 6353, 7774
BauI CACGAG 1 cut(s) 7391
BbsI GAAGAC 7 cut(s) 948, 2070, 3303, 3555, 5387, 5942, 7532
Bbv12I GWGCWC 7 cut(s) 1189, 1504, 1714, 3913, 4783, 6353, 7774
BbvCI CCTCAGC 2 cut(s) 693, 6088
BceAI ACGGC 7 cut(s) 219, 1418, 3511, 4498, 6642, 6749, 7190
BcgI CGANNNNNNTGC 4 cut(s) 3757, 3791, 4966, 5000
BciVI GTATCC 4 cut(s) 1003, 1327, 3655, 5521
BclI TGATCA 8 cut(s) 160, 1473, 2008, 2851, 3418, 3754, 5283, 7414
BcnI CCSGG 5 cut(s) 727, 728, 3676, 3677, 4250
BcoDI GTCTC 9 cut(s) 209, 393, 1244, 1805, 2129, 2385, 2801, 3279, 6433
BcuI ACTAGT 2 cut(s) 110, 2681
BfaI CTAG 8 cut(s) 111, 1761, 2682, 2781, 4652, 5141, 7605, 7814
BfmI CTRYAG 7 cut(s) 792, 1110, 2169, 3825, 4419, 4539, 5457
BfoI RGCGCY 2 cut(s) 635, 4311
BfuAI ACCTGC 6 cut(s) 1811, 2107, 3650, 3896, 5668, 5697
BfuI GTATCC 4 cut(s) 1003, 1327, 3655, 5521
BglI GCCNNNNNGGC 1 cut(s) 6298
BglII AGATCT 4 cut(s) 2165, 2843, 5375, 5427
BlpI GCTNAGC 1 cut(s) 4314
BmeT110I CYCGRG 2 cut(s) 726, 3675
BmrI ACTGGG 2 cut(s) 755, 3622
BmuI ACTGGG 2 cut(s) 755, 3622
BpiI GAAGAC 7 cut(s) 948, 2070, 3303, 3555, 5387, 5942, 7532
BplI GAGNNNNNCTC 4 cut(s) 7481, 7513, 7817, 7849
Bpu10I CCTNAGC 4 cut(s) 635, 693, 3930, 6088
Bpu1102I GCTNAGC 1 cut(s) 4314
Bpu14I TTCGAA 1 cut(s) 2522
BpuMI CCSGG 5 cut(s) 727, 728, 3676, 3677, 4250
Bsa29I ATCGAT 1 cut(s) 2341
BsaAI YACGTR 2 cut(s) 1715, 3430
BsaBI GATNNNNATC 1 cut(s) 5575
BsaHI GRCGYC 1 cut(s) 3544
BsaWI WCCGGW 5 cut(s) 4744, 6114, 6768, 6806, 7543
BsaXI ACNNNNNCTCC 4 cut(s) 3594, 3624, 3627, 3657
Bse118I RCCGGY 2 cut(s) 3876, 5034
Bse21I CCTNAGG 2 cut(s) 4158, 5292
Bse3DI GCAATG 9 cut(s) 528, 1046, 1162, 1671, 3106, 3839, 4907, 4962, 6998
Bse8I GATNNNNATC 1 cut(s) 5575
BseAI TCCGGA 3 cut(s) 4744, 6114, 7543
BseCI ATCGAT 1 cut(s) 2341
BseJI GATNNNNATC 1 cut(s) 5575
BseMI GCAATG 9 cut(s) 528, 1046, 1162, 1671, 3106, 3839, 4907, 4962, 6998
BseRI GAGGAG 4 cut(s) 819, 4674, 6266, 6408
BseSI GKGCMC 3 cut(s) 733, 1714, 4255
BseYI CCCAGC 2 cut(s) 1465, 6155
BsgI GTGCAG 4 cut(s) 725, 1071, 3816, 4040
Bsh1236I CGCG 3 cut(s) 6952, 7098, 7488
Bsh1285I CGRYCG 1 cut(s) 5812
BshNI GGYRCC 3 cut(s) 74, 1747, 6727
BshVI ATCGAT 1 cut(s) 2341
BsiEI CGRYCG 1 cut(s) 5812
BsiHKAI GWGCWC 7 cut(s) 1189, 1504, 1714, 3913, 4783, 6353, 7774
BsiHKCI CYCGRG 2 cut(s) 726, 3675
BslFI GGGAC 7 cut(s) 178, 1448, 3692, 3867, 5499, 6401, 6909
BsmAI GTCTC 9 cut(s) 209, 393, 1244, 1805, 2129, 2385, 2801, 3279, 6433
BsmFI GGGAC 7 cut(s) 178, 1448, 3692, 3867, 5499, 6401, 6909
BsmI GAATGC 2 cut(s) 6673, 6876
BsoBI CYCGRG 2 cut(s) 726, 3675
Bsp119I TTCGAA 1 cut(s) 2522
Bsp1286I GDGCHC 9 cut(s) 733, 1189, 1504, 1714, 3913, 4255, 4783, 6353, 7774
Bsp13I TCCGGA 3 cut(s) 4744, 6114, 7543
Bsp1407I TGTACA 3 cut(s) 2230, 6394, 7612
Bsp1720I GCTNAGC 1 cut(s) 4314
Bsp19I CCATGG 4 cut(s) 70, 669, 2826, 3054
BspDI ATCGAT 1 cut(s) 2341
BspEI TCCGGA 3 cut(s) 4744, 6114, 7543
BspFNI CGCG 3 cut(s) 6952, 7098, 7488
BspHI TCATGA 3 cut(s) 163, 7849, 7873
BspMAI CTGCAG 4 cut(s) 1114, 2173, 4543, 5461
BspMI ACCTGC 6 cut(s) 1811, 2107, 3650, 3896, 5668, 5697
BspQI GCTCTTC 2 cut(s) 2519, 5225
BspT104I TTCGAA 1 cut(s) 2522
BspT107I GGYRCC 3 cut(s) 74, 1747, 6727
BsrBI CCGCTC 1 cut(s) 4643
BsrDI GCAATG 9 cut(s) 528, 1046, 1162, 1671, 3106, 3839, 4907, 4962, 6998
BsrFI RCCGGY 2 cut(s) 3876, 5034
BsrGI TGTACA 3 cut(s) 2230, 6394, 7612
BssAI RCCGGY 2 cut(s) 3876, 5034
BssNAI GTATAC 3 cut(s) 2321, 7057, 7830
BssNI GRCGYC 1 cut(s) 3544
BssSI CACGAG 1 cut(s) 7391
BssT1I CCWWGG 8 cut(s) 70, 353, 669, 2106, 2826, 3054, 5194, 6916
Bst1107I GTATAC 3 cut(s) 2321, 7057, 7830
Bst2BI CACGAG 1 cut(s) 7391
BstACI GRCGYC 1 cut(s) 3544
BstAPI GCANNNNNTGC 1 cut(s) 1654
BstAUI TGTACA 3 cut(s) 2230, 6394, 7612
BstBAI YACGTR 2 cut(s) 1715, 3430
BstBI TTCGAA 1 cut(s) 2522
BstDSI CCRYGG 6 cut(s) 70, 669, 2826, 3054, 6731, 7206
BstEII GGTNACC 1 cut(s) 3779
BstENI CCTNNNNNAGG 5 cut(s) 3486, 3903, 5167, 5693, 5746
BstFNI CGCG 3 cut(s) 6952, 7098, 7488
BstH2I RGCGCY 2 cut(s) 635, 4311
BstHHI GCGC 4 cut(s) 634, 3966, 4310, 4696
BstMAI GTCTC 9 cut(s) 209, 393, 1244, 1805, 2129, 2385, 2801, 3279, 6433
BstMCI CGRYCG 1 cut(s) 5812
BstNSI RCATGY 9 cut(s) 488, 1040, 1213, 1463, 2237, 3730, 4474, 6260, 7803
BstPI GGTNACC 1 cut(s) 3779
BstSFI CTRYAG 7 cut(s) 792, 1110, 2169, 3825, 4419, 4539, 5457
BstSLI GKGCMC 3 cut(s) 733, 1714, 4255
BstUI CGCG 3 cut(s) 6952, 7098, 7488
BstV2I GAAGAC 7 cut(s) 948, 2070, 3303, 3555, 5387, 5942, 7532
BstZ17I GTATAC 3 cut(s) 2321, 7057, 7830
Bsu15I ATCGAT 1 cut(s) 2341
Bsu36I CCTNAGG 2 cut(s) 4158, 5292
BsuI GTATCC 4 cut(s) 1003, 1327, 3655, 5521
BsuTUI ATCGAT 1 cut(s) 2341
BtgI CCRYGG 6 cut(s) 70, 669, 2826, 3054, 6731, 7206
BtgZI GCGATG 1 cut(s) 6638
BtsI GCAGTG 7 cut(s) 529, 883, 1652, 2911, 5466, 5661, 6033
BveI ACCTGC 6 cut(s) 1811, 2107, 3650, 3896, 5668, 5697
CaiI CAGNNNCTG 2 cut(s) 2677, 6442
CciI TCATGA 3 cut(s) 163, 7849, 7873
CfoI GCGC 4 cut(s) 634, 3966, 4310, 4696
Cfr10I RCCGGY 2 cut(s) 3876, 5034
Cfr9I CCCGGG 2 cut(s) 726, 3675
ClaI ATCGAT 1 cut(s) 2341
CpoI CGGWCCG 1 cut(s) 6809
CseI GACGC 3 cut(s) 5255, 5402, 6863
CsiI ACCWGGT 3 cut(s) 2614, 6205, 7326
CspCI CAANNNNNGTGG 2 cut(s) 2127, 2162
CspI CGGWCCG 1 cut(s) 6809
DraI TTTAAA 2 cut(s) 2797, 7084
DraIII CACNNNGTG 3 cut(s) 1694, 5243, 6267
DrdI GACNNNNNNGTC 2 cut(s) 3559, 3989
DseDI GACNNNNNNGTC 2 cut(s) 3559, 3989
EaeI YGGCCR 4 cut(s) 1431, 3874, 3892, 5074
Ecl136II GAGCTC 2 cut(s) 6351, 7772
Eco130I CCWWGG 8 cut(s) 70, 353, 669, 2106, 2826, 3054, 5194, 6916
Eco147I AGGCCT 2 cut(s) 3622, 4156
Eco24I GRGCYC 2 cut(s) 6353, 7774
Eco32I GATATC 2 cut(s) 1120, 4147
Eco47III AGCGCT 1 cut(s) 633
Eco53kI GAGCTC 2 cut(s) 6351, 7772
Eco81I CCTNAGG 2 cut(s) 4158, 5292
Eco88I CYCGRG 2 cut(s) 726, 3675
Eco91I GGTNACC 1 cut(s) 3779
EcoICRI GAGCTC 2 cut(s) 6351, 7772
EcoNI CCTNNNNNAGG 5 cut(s) 3486, 3903, 5167, 5693, 5746
EcoO109I RGGNCCY 1 cut(s) 2303
EcoO65I GGTNACC 1 cut(s) 3779
EcoRI GAATTC 3 cut(s) 4009, 5761, 6220
EcoRV GATATC 2 cut(s) 1120, 4147
EcoT14I CCWWGG 8 cut(s) 70, 353, 669, 2106, 2826, 3054, 5194, 6916
EcoT22I ATGCAT 2 cut(s) 486, 6673
EcoT38I GRGCYC 2 cut(s) 6353, 7774
ErhI CCWWGG 8 cut(s) 70, 353, 669, 2106, 2826, 3054, 5194, 6916
FalI AAGNNNNNCTT 8 cut(s) 618, 650, 2516, 2548, 4860, 4892, 5773, 5805
FaqI GGGAC 7 cut(s) 178, 1448, 3692, 3867, 5499, 6401, 6909
FauI CCCGC 1 cut(s) 3810
FauNDI CATATG 2 cut(s) 3751, 7765
FbaI TGATCA 8 cut(s) 160, 1473, 2008, 2851, 3418, 3754, 5283, 7414
FblI GTMKAC 5 cut(s) 2320, 3517, 7056, 7401, 7829
FriOI GRGCYC 2 cut(s) 6353, 7774
FspBI CTAG 8 cut(s) 111, 1761, 2682, 2781, 4652, 5141, 7605, 7814
FspI TGCGCA 1 cut(s) 3965
GlaI GCGC 4 cut(s) 633, 3965, 4309, 4695
GsaI CCCAGC 2 cut(s) 1469, 6159
HaeII RGCGCY 2 cut(s) 635, 4311
HgaI GACGC 3 cut(s) 5255, 5402, 6863
HhaI GCGC 4 cut(s) 634, 3966, 4310, 4696
Hin1I GRCGYC 1 cut(s) 3544
Hin6I GCGC 4 cut(s) 632, 3964, 4308, 4694
HinP1I GCGC 4 cut(s) 632, 3964, 4308, 4694
HincII GTYRAC 5 cut(s) 784, 3982, 4345, 4819, 5590
HindII GTYRAC 5 cut(s) 784, 3982, 4345, 4819, 5590
HindIII AAGCTT 3 cut(s) 983, 2409, 6611
Hpy99I CGWCG 5 cut(s) 33, 3546, 3549, 5418, 6834
HpyCH4IV ACGT 6 cut(s) 31, 98, 1714, 3429, 3544, 7277
HpySE526I ACGT 6 cut(s) 31, 98, 1714, 3429, 3544, 7277
Hsp92I GRCGYC 1 cut(s) 3544
HspAI GCGC 4 cut(s) 632, 3964, 4308, 4694
Kpn2I TCCGGA 3 cut(s) 4744, 6114, 7543
KpnI GGTACC 1 cut(s) 78
Ksp22I TGATCA 8 cut(s) 160, 1473, 2008, 2851, 3418, 3754, 5283, 7414
LguI GCTCTTC 2 cut(s) 2519, 5225
MabI ACCWGGT 3 cut(s) 2614, 6205, 7326
MaeI CTAG 8 cut(s) 111, 1761, 2682, 2781, 4652, 5141, 7605, 7814
MaeII ACGT 6 cut(s) 31, 98, 1714, 3429, 3544, 7277
MbiI CCGCTC 1 cut(s) 4643
MfeI CAATTG 4 cut(s) 333, 3959, 4599, 5070
MhlI GDGCHC 9 cut(s) 733, 1189, 1504, 1714, 3913, 4255, 4783, 6353, 7774
MlsI TGGCCA 1 cut(s) 3894
MluNI TGGCCA 1 cut(s) 3894
MlyI GAGTC 9 cut(s) 211, 1157, 1246, 1426, 2159, 4969, 5073, 5199, 6557
MmeI TCCRAC 8 cut(s) 1788, 1876, 5041, 5396, 5839, 6386, 6678, 6997
Mox20I TGGCCA 1 cut(s) 3894
Mph1103I ATGCAT 2 cut(s) 486, 6673
MroI TCCGGA 3 cut(s) 4744, 6114, 7543
MroXI GAANNNNTTC 1 cut(s) 7479
MscI TGGCCA 1 cut(s) 3894
MslI CAYNNNNRTG 5 cut(s) 2715, 3114, 3386, 5358, 7395
Msp20I TGGCCA 1 cut(s) 3894
MspA1I CMGCKG 4 cut(s) 2014, 2870, 6092, 6814
MunI CAATTG 4 cut(s) 333, 3959, 4599, 5070
Mva1269I GAATGC 2 cut(s) 6673, 6876
MvnI CGCG 3 cut(s) 6952, 7098, 7488
NciI CCSGG 5 cut(s) 727, 728, 3676, 3677, 4250
NcoI CCATGG 4 cut(s) 70, 669, 2826, 3054
NdeI CATATG 2 cut(s) 3751, 7765
NsbI TGCGCA 1 cut(s) 3965
NsiI ATGCAT 2 cut(s) 486, 6673
NspI RCATGY 9 cut(s) 488, 1040, 1213, 1463, 2237, 3730, 4474, 6260, 7803
NspV TTCGAA 1 cut(s) 2522
PaeI GCATGC 1 cut(s) 6260
PagI TCATGA 3 cut(s) 163, 7849, 7873
PaqCI CACCTGC 2 cut(s) 3650, 3896
PceI AGGCCT 2 cut(s) 3622, 4156
PciI ACATGT 3 cut(s) 1036, 1209, 3726
PciSI GCTCTTC 2 cut(s) 2519, 5225
PctI GAATGC 2 cut(s) 6673, 6876
PdmI GAANNNNTTC 1 cut(s) 7479
PflFI GACNNNGTC 1 cut(s) 3546
PflMI CCANNNNNTGG 4 cut(s) 675, 3055, 3872, 6267
PfoI TCCNGGA 3 cut(s) 448, 2305, 6427
PleI GAGTC 9 cut(s) 211, 1156, 1246, 1425, 2158, 4968, 5072, 5199, 6556
PpsI GAGTC 9 cut(s) 211, 1156, 1246, 1425, 2158, 4968, 5072, 5199, 6556
Ppu21I YACGTR 2 cut(s) 1715, 3430
PpuMI RGGWCCY 1 cut(s) 2303
PscI ACATGT 3 cut(s) 1036, 1209, 3726
PshBI ATTAAT 3 cut(s) 54, 5328, 7449
PsiI TTATAA 2 cut(s) 3330, 3617
Psp124BI GAGCTC 2 cut(s) 6353, 7774
Psp1406I AACGTT 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 2303
PspEI GGTNACC 1 cut(s) 3779
PspFI CCCAGC 2 cut(s) 1465, 6155
PspPPI RGGWCCY 1 cut(s) 2303
PsrI GAACNNNNNNTAC 2 cut(s) 2178, 2210
PstI CTGCAG 4 cut(s) 1114, 2173, 4543, 5461
PstNI CAGNNNCTG 2 cut(s) 2677, 6442
PsyI GACNNNGTC 1 cut(s) 3546
PvuII CAGCTG 3 cut(s) 2014, 2870, 6092
RseI CAYNNNNRTG 5 cut(s) 2715, 3114, 3386, 5358, 7395
Rsr2I CGGWCCG 1 cut(s) 6809
RsrII CGGWCCG 1 cut(s) 6809
SacI GAGCTC 2 cut(s) 6353, 7774
SapI GCTCTTC 2 cut(s) 2519, 5225
SchI GAGTC 9 cut(s) 211, 1157, 1246, 1426, 2159, 4969, 5073, 5199, 6557
SduI GDGCHC 9 cut(s) 733, 1189, 1504, 1714, 3913, 4255, 4783, 6353, 7774
SexAI ACCWGGT 3 cut(s) 2614, 6205, 7326
SfcI CTRYAG 7 cut(s) 792, 1110, 2169, 3825, 4419, 4539, 5457
SfuI TTCGAA 1 cut(s) 2522
SmaI CCCGGG 2 cut(s) 728, 3677
SmiMI CAYNNNNRTG 5 cut(s) 2715, 3114, 3386, 5358, 7395
SpeI ACTAGT 2 cut(s) 110, 2681
SphI GCATGC 1 cut(s) 6260
SrfI GCCCGGGC 1 cut(s) 728
SseBI AGGCCT 2 cut(s) 3622, 4156
SspI AATATT 4 cut(s) 1380, 2792, 6280, 6382
SspMI CTAG 8 cut(s) 111, 1761, 2682, 2781, 4652, 5141, 7605, 7814
SstI GAGCTC 2 cut(s) 6353, 7774
StuI AGGCCT 2 cut(s) 3622, 4156
StyI CCWWGG 8 cut(s) 70, 353, 669, 2106, 2826, 3054, 5194, 6916
TaiI ACGT 6 cut(s) 34, 101, 1717, 3432, 3547, 7280
TatI WGTACW 9 cut(s) 1070, 1939, 2230, 2574, 4268, 5888, 6394, 7581, 7612
TauI GCSGC 4 cut(s) 854, 3267, 4736, 7098
TspGWI ACGGA 4 cut(s) 1352, 1910, 7079, 7391
TspMI CCCGGG 2 cut(s) 726, 3675
Tth111I GACNNNGTC 1 cut(s) 3546
Van91I CCANNNNNTGG 4 cut(s) 675, 3055, 3872, 6267
VneI GTGCAC 1 cut(s) 1710
VspI ATTAAT 3 cut(s) 54, 5328, 7449
XagI CCTNNNNNAGG 5 cut(s) 3486, 3903, 5167, 5693, 5746
XbaI TCTAGA 2 cut(s) 1760, 5140
XceI RCATGY 9 cut(s) 488, 1040, 1213, 1463, 2237, 3730, 4474, 6260, 7803
XcmI CCANNNNNNNNNTGG 4 cut(s) 598, 1691, 3308, 5342
XmaI CCCGGG 2 cut(s) 726, 3675
XmiI GTMKAC 5 cut(s) 2320, 3517, 7056, 7401, 7829
XmnI GAANNNNTTC 1 cut(s) 7479
XspI CTAG 8 cut(s) 111, 1761, 2682, 2781, 4652, 5141, 7605, 7814
ZraI GACGTC 1 cut(s) 3545
Zsp2I ATGCAT 2 cut(s) 486, 6673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.