pycom03g02420

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007599
Physical Location & Seq
Forward (+)
1825266 .. 1829199
3934 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1317 bp
ATGGGGAGCGCCAGAGACCGAGACGACTCACTCGCATTCACCAACCCATCCACGTCCTCCTCCCCAATCGTCATCTCCGACGCGCTCGAAAGCTTCCTCTCCGACCCCAATTCCCACATCGGCAGCGCCTCCGGGAGCTTCCAGAACGAGGGTCTCCTCGCCGACACCAGCGGAAGTTGCAGCGACGCCGAGTTGGGGTTCTCCCGGTCCGAGTTCCGGACGAGCCAACTCGCCGGGACGGTGGAGTTTTACCAAAGGCATGTGTTTTTGTGCTACAAGAACCCGCAGGTTTGGCCGCCGAGGATCGAGGCCGCCGAGTTTGATCGGCTGCCGAGGTTGCTCTACTCCGCCGTCATGGCCAGGAGGGCGGATATGAAGAAAGAGACCCGCTTAACAATATGTGAAGGACATGATGGAACTGAAACATCAAATGGCGATGTATTAATTTTCCCTGACATGATCCGATACAGGAGATTGACCCATTTTGATGTTGATACATTTGTTGAAGAAGTTCTTGTGAAGGATGGTGAATGGCTGCCTGGAACTCCTGAAACTCTGAAGGGTTCATATGTTTTTGTATGTTCTCATGGGTCCCGGGATCGCCGTTGTGGAGTCTGTGGACCTCCCCTGATCAATAGATTTAGAGAAGAGATAGAATTGCATGGTCTTCAGGGTAAAGTGTCTGTTAGCCCATGTTCGCATATTGGGGGGCATAAATATGCTGGAAATGTTATCATATTTGGATCAAATAGCAACAGAGAAGTCTCGGGCCACTGGTATGGATATGTTGCTCCAGAAGATGTAAATGTATTGCTTGAACAGCATGTCGGGAAAGGAGAAATTGTAGACTGGCTATGGAGGGGTCAGATGGGTTTATCAGAAGAACAGCAGAAGGAATCTCAAGAACGAAGGCTCGAGCTTAATGGTGAGACAGACGTGGGAAAAACCACTACAGAGTTGACACAACCAAAGGAAAGGGGGATGAATACTAGTGTCTGTAGATCGCAAGTCGAGAATGGGGGCTGTTGCCAGGAAAACGGAAACTCTTGCTGTCAGAATGCTACGCTCATGGAAAACATAAATAGTCCCGACCTGAATGATGAGATTGCAGTGAAGGCAACAGCCGAAAAGAAAAAAGGCAGTAGGAAACCACTTTCACGAATGAATAGTGGCAAAGGGGCATCCACGCGCAAGGTTTGTGCGATACCAACATGGTTTCAGAGCTGGGAGCGTGAAGATACATACGCAGCTGTAGCTGTTGTTTGTGCTGCTGTGTCAGTTGCCGTTGCTTACAAGTGCTACAAACAGTTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

439

Amino Acids

48.44

Weight (kDa)

5.69

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 77 - 288 1.9e-46 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 846
AccII CGCG 2 cut(s) 83, 1189
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 7 cut(s) 171, 284, 296, 312, 348, 368, 388
AclWI GGATC 4 cut(s) 311, 454, 606, 751
AcoI YGGCCR 2 cut(s) 293, 357
AcuI CTGAAG 2 cut(s) 578, 653
AcyI GRCGYC 1 cut(s) 186
AfiI CCNNNNNNNGG 3 cut(s) 148, 195, 216
AgsI TTSAA 2 cut(s) 506, 818
AhlI ACTAGT 1 cut(s) 989
AjiI CACGTC 2 cut(s) 54, 937
AjnI CCWGG 3 cut(s) 359, 538, 1029
AluBI AGCT 7 cut(s) 93, 138, 919, 1224, 1250, 1256, 1314
AluI AGCT 7 cut(s) 93, 138, 919, 1224, 1250, 1256, 1314
Alw26I GTCTC 6 cut(s) 9, 15, 158, 377, 769, 923
AlwI GGATC 4 cut(s) 311, 454, 606, 751
Ama87I CYCGRG 3 cut(s) 594, 766, 914
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 4 cut(s) 293, 309, 357, 769
ApeKI GCWGC 6 cut(s) 123, 180, 328, 535, 1247, 1268
AseI ATTAAT 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 4 cut(s) 11, 85, 128, 1191
AspS9I GGNCC 4 cut(s) 207, 591, 620, 769
AsuC2I CCSGG 5 cut(s) 133, 205, 235, 595, 596
AsuHPI GGTGA 3 cut(s) 31, 539, 938
AvaI CYCGRG 3 cut(s) 594, 766, 914
AvaII GGWCC 3 cut(s) 207, 591, 620
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BalI TGGCCA 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 659
BbvI GCAGC 6 cut(s) 135, 192, 315, 522, 1255, 1259
BccI CCATC 4 cut(s) 55, 407, 518, 862
BceAI ACGGC 3 cut(s) 335, 588, 1268
BciT130I CCWGG 3 cut(s) 361, 540, 1031
BclI TGATCA 1 cut(s) 630
BcnI CCSGG 5 cut(s) 133, 205, 235, 595, 596
BcoDI GTCTC 6 cut(s) 9, 15, 158, 377, 769, 923
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 3 cut(s) 951, 997, 1251
BfoI RGCGCY 2 cut(s) 12, 129
BfuAI ACCTGC 1 cut(s) 277
BglI GCCNNNNNGGC 2 cut(s) 356, 365
BisI GCNGC 8 cut(s) 124, 181, 296, 312, 329, 536, 1248, 1269
BlsI GCNGC 8 cut(s) 125, 182, 297, 313, 330, 537, 1249, 1270
Bme1390I CCNGG 8 cut(s) 133, 205, 235, 361, 540, 595, 596, 1031
Bme18I GGWCC 3 cut(s) 207, 591, 620
BmeT110I CYCGRG 3 cut(s) 594, 766, 914
BmgBI CACGTC 2 cut(s) 54, 937
BmgT120I GGNCC 4 cut(s) 207, 591, 620, 769
BmiI GGNNCC 2 cut(s) 592, 593
BmrFI CCNGG 8 cut(s) 133, 205, 235, 361, 540, 595, 596, 1031
BmsI GCATC 1 cut(s) 1190
BpiI GAAGAC 1 cut(s) 659
BpmI CTGGAG 1 cut(s) 777
BpuEI CTTGAG 1 cut(s) 885
BpuMI CCSGG 5 cut(s) 133, 205, 235, 595, 596
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 3 cut(s) 9, 158, 377
BsaJI CCNNGG 3 cut(s) 299, 332, 594
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 195, 216
Bse1I ACTGG 2 cut(s) 779, 854
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 3 cut(s) 361, 540, 1031
BseDI CCNNGG 3 cut(s) 299, 332, 594
BseGI GGATG 4 cut(s) 47, 529, 987, 1181
BseLI CCNNNNNNNGG 3 cut(s) 148, 195, 216
BseNI ACTGG 2 cut(s) 779, 854
BseRI GAGGAG 2 cut(s) 49, 146
BseXI GCAGC 6 cut(s) 135, 192, 315, 522, 1255, 1259
BseYI CCCAGC 1 cut(s) 1224
Bsh1236I CGCG 2 cut(s) 83, 1189
BshFI GGCC 4 cut(s) 295, 311, 359, 771
BsiHKCI CYCGRG 3 cut(s) 594, 766, 914
BsiSI CCGG 5 cut(s) 132, 205, 217, 234, 595
BslFI GGGAC 3 cut(s) 250, 577, 1071
BslI CCNNNNNNNGG 3 cut(s) 148, 195, 216
BsmAI GTCTC 6 cut(s) 9, 15, 158, 377, 769, 923
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 3 cut(s) 250, 577, 1071
BsmI GAATGC 2 cut(s) 35, 1063
BsnI GGCC 4 cut(s) 295, 311, 359, 771
Bso31I GGTCTC 3 cut(s) 9, 158, 377
BsoBI CYCGRG 3 cut(s) 594, 766, 914
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
BspACI CCGC 7 cut(s) 171, 284, 296, 312, 348, 368, 388
BspANI GGCC 4 cut(s) 295, 311, 359, 771
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 2 cut(s) 83, 1189
BspLI GGNNCC 2 cut(s) 592, 593
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 4 cut(s) 311, 454, 606, 751
BspTNI GGTCTC 3 cut(s) 9, 158, 377
BsrI ACTGG 2 cut(s) 779, 854
BssECI CCNNGG 3 cut(s) 299, 332, 594
BssMI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 361, 540, 1031
Bst4CI ACNGT 2 cut(s) 241, 1308
Bst6I CTCTTC 1 cut(s) 642
BstACI GRCGYC 1 cut(s) 186
BstF5I GGATG 4 cut(s) 47, 529, 987, 1181
BstFNI CGCG 2 cut(s) 83, 1189
BstH2I RGCGCY 2 cut(s) 12, 129
BstHHI GCGC 4 cut(s) 11, 85, 128, 1191
BstKTI GATC 7 cut(s) 306, 325, 462, 601, 633, 746, 1004
BstMAI GTCTC 6 cut(s) 9, 15, 158, 377, 769, 923
BstMBI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
BstMWI GCNNNNNNNGC 8 cut(s) 177, 292, 337, 356, 365, 820, 1115, 1253
BstNI CCWGG 3 cut(s) 361, 540, 1031
BstNSI RCATGY 2 cut(s) 263, 827
BstSCI CCNGG 8 cut(s) 131, 203, 233, 359, 538, 593, 594, 1029
BstSFI CTRYAG 3 cut(s) 951, 997, 1251
BstUI CGCG 2 cut(s) 83, 1189
BstV1I GCAGC 6 cut(s) 135, 192, 315, 522, 1255, 1259
BstV2I GAAGAC 1 cut(s) 659
BstXI CCANNNNNNTGG 1 cut(s) 779
BsuRI GGCC 4 cut(s) 295, 311, 359, 771
BtgZI GCGATG 1 cut(s) 450
BtrI CACGTC 2 cut(s) 54, 937
BtsCI GGATG 4 cut(s) 47, 529, 987, 1181
BtsI GCAGTG 1 cut(s) 1116
BtsIMutI CAGTG 2 cut(s) 772, 1116
BveI ACCTGC 1 cut(s) 277
CfoI GCGC 4 cut(s) 11, 85, 128, 1191
Cfr13I GGNCC 4 cut(s) 207, 591, 620, 769
Cfr9I CCCGGG 1 cut(s) 594
CpoI CGGWCCG 1 cut(s) 207
CseI GACGC 2 cut(s) 89, 194
CspI CGGWCCG 1 cut(s) 207
DpnI GATC 7 cut(s) 305, 324, 461, 600, 632, 745, 1003
DpnII GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
EaeI YGGCCR 2 cut(s) 293, 357
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 2 cut(s) 337, 383
Eco31I GGTCTC 3 cut(s) 9, 158, 377
Eco47I GGWCC 3 cut(s) 207, 591, 620
Eco57I CTGAAG 2 cut(s) 578, 653
Eco88I CYCGRG 3 cut(s) 594, 766, 914
EcoO109I RGGNCCY 1 cut(s) 591
EcoRII CCWGG 3 cut(s) 359, 538, 1029
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 3 cut(s) 250, 577, 1071
FauI CCCGC 2 cut(s) 291, 395
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 1 cut(s) 630
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 8 cut(s) 124, 181, 296, 312, 329, 536, 1248, 1269
FokI GGATG 4 cut(s) 34, 536, 994, 1168
Fsp4HI GCNGC 8 cut(s) 124, 181, 296, 312, 329, 536, 1248, 1269
FspBI CTAG 1 cut(s) 990
GlaI GCGC 4 cut(s) 10, 84, 127, 1190
GluI GCNGC 8 cut(s) 124, 181, 296, 312, 329, 536, 1248, 1269
GsaI CCCAGC 1 cut(s) 1228
GsuI CTGGAG 1 cut(s) 777
HaeII RGCGCY 2 cut(s) 12, 129
HaeIII GGCC 4 cut(s) 295, 311, 359, 771
HapII CCGG 5 cut(s) 132, 205, 217, 234, 595
HgaI GACGC 2 cut(s) 89, 194
HhaI GCGC 4 cut(s) 11, 85, 128, 1191
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 4 cut(s) 9, 83, 126, 1189
HinP1I GCGC 4 cut(s) 9, 83, 126, 1189
HincII GTYRAC 1 cut(s) 960
HindII GTYRAC 1 cut(s) 960
HindIII AAGCTT 1 cut(s) 91
HinfI GANTC 3 cut(s) 26, 612, 896
HpaII CCGG 5 cut(s) 132, 205, 217, 234, 595
HphI GGTGA 3 cut(s) 31, 539, 938
Hpy166II GTNNAC 3 cut(s) 620, 847, 960
Hpy188I TCNGA 9 cut(s) 79, 103, 211, 464, 558, 867, 880, 1056, 1221
Hpy188III TCNNGA 9 cut(s) 142, 217, 548, 794, 829, 902, 1012, 1088, 1158
Hpy8I GTNNAC 3 cut(s) 620, 847, 960
Hpy99I CGWCG 2 cut(s) 83, 188
HpyAV CCTTC 6 cut(s) 398, 514, 553, 886, 903, 1108
HpyCH4III ACNGT 2 cut(s) 241, 1308
HpyCH4IV ACGT 2 cut(s) 53, 936
HpyCH4V TGCA 3 cut(s) 180, 661, 1109
HpyF10VI GCNNNNNNNGC 8 cut(s) 177, 292, 337, 356, 365, 820, 1115, 1253
HpySE526I ACGT 2 cut(s) 53, 936
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 4 cut(s) 9, 83, 126, 1189
KflI GGGWCCC 1 cut(s) 591
Kpn2I TCCGGA 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 630
Kzo9I GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
LmnI GCTCC 4 cut(s) 6, 135, 796, 1228
Lsp1109I GCAGC 6 cut(s) 135, 192, 315, 522, 1255, 1259
LweI GCATC 1 cut(s) 1190
MaeI CTAG 1 cut(s) 990
MaeII ACGT 2 cut(s) 53, 936
MalI GATC 7 cut(s) 305, 324, 461, 600, 632, 745, 1003
MboI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
MboII GAAGA 7 cut(s) 388, 518, 659, 659, 809, 893, 1247
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 4 cut(s) 109, 444, 656, 840
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 2 cut(s) 20, 621
MmeI TCCRAC 2 cut(s) 102, 126
Mox20I TGGCCA 1 cut(s) 359
MroI TCCGGA 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 510
MscI TGGCCA 1 cut(s) 359
MseI TTAA 3 cut(s) 392, 443, 921
MslI CAYNNNNRTG 3 cut(s) 486, 717, 777
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 2 cut(s) 171, 1250
MspI CCGG 5 cut(s) 132, 205, 217, 234, 595
MspR9I CCNGG 8 cut(s) 133, 205, 235, 361, 540, 595, 596, 1031
Mva1269I GAATGC 2 cut(s) 35, 1063
MvaI CCWGG 3 cut(s) 361, 540, 1031
MvnI CGCG 2 cut(s) 83, 1189
MwoI GCNNNNNNNGC 8 cut(s) 177, 292, 337, 356, 365, 820, 1115, 1253
NciI CCSGG 5 cut(s) 133, 205, 235, 595, 596
NdeI CATATG 1 cut(s) 568
NdeII GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
NlaIV GGNNCC 2 cut(s) 592, 593
NmeAIII GCCGAG 4 cut(s) 214, 324, 340, 357
NspI RCATGY 2 cut(s) 263, 827
PaeR7I CTCGAG 1 cut(s) 914
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 312
PctI GAATGC 2 cut(s) 35, 1063
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 1 cut(s) 896
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 8 cut(s) 125, 182, 297, 313, 330, 537, 1249, 1270
PleI GAGTC 2 cut(s) 20, 620
PpsI GAGTC 2 cut(s) 20, 620
PpuMI RGGWCCY 1 cut(s) 591
PshBI ATTAAT 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 359, 538, 1029
PspFI CCCAGC 1 cut(s) 1224
PspGI CCWGG 3 cut(s) 359, 538, 1029
PspN4I GGNNCC 2 cut(s) 592, 593
PspPI GGNCC 4 cut(s) 207, 591, 620, 769
PspPPI RGGWCCY 1 cut(s) 591
PspXI VCTCGAGB 1 cut(s) 914
PvuII CAGCTG 1 cut(s) 1250
RseI CAYNNNNRTG 3 cut(s) 486, 717, 777
Rsr2I CGGWCCG 1 cut(s) 207
RsrII CGGWCCG 1 cut(s) 207
SaqAI TTAA 3 cut(s) 392, 443, 921
SatI GCNGC 8 cut(s) 124, 181, 296, 312, 329, 536, 1248, 1269
Sau3AI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
Sau96I GGNCC 4 cut(s) 207, 591, 620, 769
SchI GAGTC 2 cut(s) 20, 621
ScrFI CCNGG 8 cut(s) 133, 205, 235, 361, 540, 595, 596, 1031
SfaNI GCATC 1 cut(s) 1190
SfcI CTRYAG 3 cut(s) 951, 997, 1251
Sfr274I CTCGAG 1 cut(s) 914
SinI GGWCC 3 cut(s) 207, 591, 620
SlaI CTCGAG 1 cut(s) 914
SmaI CCCGGG 1 cut(s) 596
SmiMI CAYNNNNRTG 3 cut(s) 486, 717, 777
SmlI CTYRAG 2 cut(s) 900, 914
SmoI CTYRAG 2 cut(s) 900, 914
SpeI ACTAGT 1 cut(s) 989
Sse9I AATT 4 cut(s) 109, 444, 656, 840
SsiI CCGC 7 cut(s) 171, 284, 296, 312, 348, 368, 388
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 8 cut(s) 131, 203, 233, 359, 538, 593, 594, 1029
TaaI ACNGT 2 cut(s) 241, 1308
TaiI ACGT 2 cut(s) 56, 939
TaqI TCGA 4 cut(s) 87, 306, 915, 1011
TaqII GACCGA 1 cut(s) 33
TasI AATT 4 cut(s) 109, 444, 656, 840
TauI GCSGC 2 cut(s) 298, 314
TfiI GAWTC 1 cut(s) 896
Tru1I TTAA 3 cut(s) 392, 443, 921
Tru9I TTAA 3 cut(s) 392, 443, 921
TscAI CASTG 2 cut(s) 779, 1116
TseI GCWGC 6 cut(s) 123, 180, 328, 535, 1247, 1268
TspDTI ATGAA 4 cut(s) 389, 555, 998, 1178
TspGWI ACGGA 1 cut(s) 1053
TspMI CCCGGG 1 cut(s) 594
TspRI CASTG 2 cut(s) 779, 1116
VpaK11BI GGWCC 3 cut(s) 207, 591, 620
VspI ATTAAT 1 cut(s) 443
XceI RCATGY 2 cut(s) 263, 827
XhoI CTCGAG 1 cut(s) 914
XmaI CCCGGG 1 cut(s) 594
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.