Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

11308124 .. 11315556

Gene Structure 7433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4143 bp

ATGGCCGACGAAAGAGAAGCTGCAGGAAGCGGTGGCAGCCTGGTTTTTGCAGTTAACGGGGAGAGGTTTGAGCTCTCGACCCTTGACCCTTCCACCACTCTCCTCGACTTCTTGCGTTGTCACACCACATTCAAAAGTGTCAAGCTCAGCTGCGGCGAAGGTGGTTGCGGTGCTTGTGTTGTGCTTCTGTCCAAGTACGACCCTGTTGCGGACCAAGTGAAAGATTTTACTGTAAGTTCATGTCTCACTCTGCTTTGCAGTGTAAATGGAGGTTCAATTACAACATCCGAAGGCCTTGGAAATCTCAAAGACGGGTTCCACCCAATTCACCAAAGGATTACTGGTTTCCATGCTTCTCAATGTGGCTTTTGTACTCCTGGCATGTGTGTTTCACTCTTTGGTGCTCTGGTCAATGCTAAAAAGACGAATAGACCTGAACCCCCTCATGGGTTCTCCAAGCTAACTGTTTCCGAAGCTGAAAAGTCTATTGCAGGTAACCTCTGTCGATGTACTGGGTATCGATCAATTGCTGATGCCTGCAAGAGTTTCGCAGCTGATGTTGATATGGAGGATTTGGGGTTCAATTATTTTTGGAAGAAGGGAGAGAGTAAGGAAGTAAAGATAAGTAGATTGCCCCTATATAACCATAACAATGACATCTGTACATTTCCGGACTTCTTAAAAAATGAAATCCGCTCTTCTATGTCTTTGGATCCAAAGAGATATTGCTGGTACATTCCTGTTAGAGTTGAGGAGCTTCAAAACTTATTGACAGCTACTGATTTTTATAATGCGGACGATATGAAATTAGTTGTTGGAAACACAGGGACGGGCTATTACAAGGAGCTAAAGCGCTATGACAGATACATTGATCTCAGATATGTTCCTGAATTATCAATGATTAAGATAGATCCGACAGGGGTTGAGTTTGGAGCAATTGTAACAATCTCTAAGGTTATTGAAGCTTTGAGGAAGAAGGACAACGGTGAATCTCCATCAAGAGGTGAGGTTGTGTTGAAAAAAATTGCCAATCATATGGGGAAAATTGCTTCAGGGTTCATCAGAAATACAGCTAGTATAGGAGGGAATTTGGTGATGACACAGAGAAAATGCTTTCCTTCAGATATTGCTACAATACTTCTTGCTGTGGATTCAGAGGTAAATATAATGGATGGTTCTAGATCTGAAACGATTATGTTGGAGGATTTTCTGAAACAGTCTCCACTGGATCCAAAAAGTGTACTTCTAAGTGTTAAAATCCCAAAATGGGAAGCAGTCAGAAACGTTTCTCCAGAAACCAACACTGTATTGCTGTTTGAAACCTATCGAGCTGCACCAAGACCCCTAGGAAACGCATTGGCTTATTTAAATGCTGCCTTCCTAGCTGAAGTATCGTTTTGTAAGATTTCCAATGGAATTATGGTGGATAATTGTCGTCTGGCTTTTGGTGCTTATGGAACTAAATATGCAATTAGAGCAAGAAAGGCTGAGGAACTTTTAACTGGGAAAGTGCTGAGTCCTGGTGTTCTGTATGACGCTATTAAATTAGTTAAAGAAGTTGTGGTTCCCGAAGAAGGAACTACAAGCCCTGCTTACAGGACAAGCTTGGCTGCTGGTTTTCTTTTCGAGTTCTTTAGCCCCTTAATTGACAGTGAATATGATATTTCCAATGATTTCTTGGGAACTACTTTGTTGGCCGATTCTTCTAAGTTAAAAAGGAACCAAGGTGCTAATGATGAGATGACAACTGTTCTGTCATCTGCTAAACAGGTGCTTGAATTAAGTACAGAGTATGATCCGGTTGGTAAGCCAATTACAAAATCTGGAGGTCTCATACAAGCTTCAGGTGAGGCAGTCTATGTAGACGACATTCCGTCTCCGAAAAATTGCTTACATGGAGCGTTCATTTATAGTACAAAGCCTTTGGCTAGAGTGAAGAGAATAAATTTTGAACCTAAACGACATCCAGAAGGAGTTGCTGCACTTATATCTTTTAAAGATATTCCCAAGTCTGGGGAGAACATAGGGTCTAAGACTATATTTGGTACAGAACCTCTTTTCGCTGACGATCTTACCGAATGTGCTGGTCAGCGTCTTGCTTTCGTGGTAGCCGATACGCAGAAACATGCAGACTTGGCAACAAACTTTGTGGTGGTTGATTATGACATGGAAGATATAGACCCACCTATTCTATCAGTCGAAGAGGCAGTTAAGAGATCTAATTTTTTTGAGGTCCCTCCTTTCTTGTATCCGAAACAGGTTGGTGACATATCAAATGGAATGGCTGCAGCTGATCACAAGATTATTTCTGCTGAGATCAAACTTGGTTCACAATACTATTTCTATATGGAGACACAAACTGCCCTTGCTGTGCCAGATGAAGACAACTGCATGGTGGTTTACACTTCAAGTCAGTGCCCTGAGATTGCACACTCTTCAATTGCAAAATGCCTTGGTATTCCTGAAAATAATGTTCGTGTAATTACAAGAAGGGTTGGAGGGGGCTTTGGTGGAAAGGCCATAAAGTCCATGCCTGTGGCAACTGCGTGTGCTCTTGCTGCACACAAATTACATAGACCTGTAAGGATGTATCTCAATCGGAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCATGAAGATAACTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACTGCTCTAGATCTTGAGATATTAATAAATGCAGGGATTTCCCTAGATATAAGTCCAGTAATGCCAAACAATATTCTGTCTGCACTTAAGAAGTATGACTGGGGTGCTCTAGCTTTCGATTTCAAGGTGTGTAAAACAAACACTCCAAGTAGGTCTGCTATGCGGGCCCCTGGGGAGGTGCAGGGGTCTTTTATTGCTGAAGCTGTAATTGAACATGTAGCATCCACCCTTTCTATAGAAGTGGATTCTGTGAGAAGCATTAATCTTCACACCTCTCACAGCCTTGATTTGTTTTATGAGCACAGCGCTGGTGAACCTCTGGAGTATACATTACCGTTTATATGGGACAAGTTGGCCATGTCTTCAAGCTTTAACCCAAGGACTGAGATGGTAAAAGAATTTAATAGGTGTAATAAATGGCAGAAAAGAGGAATTTCTCGAGTACCGATATTGCATGAAGTGATACTGAGGCCAACTCCAGCAAGAGTAAGCATTTTAGGTGATGGGTCTGTTGTCGTTGAAGTTGGAGGAATTGAACTGGGTCAGGGGCTCTGGACGAAGGTAAAACAGATGGCAGCTTTTGCTCTTGGTTCAATTCAATGTGATGGTAGTGGAGACCTTTTGGATAAAGTACGGGTGGTGCAATCTGATACATTGAGCTTAATTCAAGGGGGATTTACTTCCGGGAGCACTACATCTGAGGCAAGCTGTGAGGCAGTTAGACTTTGCTGTAATATTTTGGTTGAGAGGTTAGCTACTCTCAAGGGGAGATTGAAGGAGCAAATGGGCTCTGTTAACTGGGAAACTCTTATTCAGCAGGCATCCTTGGAAGCTGTGAACTTATCAGCAAGTTCTTACTATGTCCCTGATTTTGCTTCCATGAAGTACCTTAACTATGGCGCTGCTGTGAGTGAGGTGGAGGTAAATGTCCTGACTGGAGAAACCAGAATTCTGCGATCAGATATTATCTACGATTGTGGTCAGAGTCTCAACCCGGCTGTGGACTTAGGACAGATCGAAGGTGCTTTTGTTCAAGGTATTGGGTTTTTCATGCTTGAAGAATACTTGGAAAACTCAGATGGGTTGGTGATTTCAGAAGGTACGTGGACTTATAAAATTCCTACTGTGGACACCATACCCAAACAGTTCAACGTTGAAGTATTGAACAGTGGGCATCACAAAAAACGCGTGCTCTCTTCAAAAGCTTCTGGTGAGCCACCATTACTTCTTGCGGTTTCTGTTCACTGTGCTACAAGGGCTGCCATTAAAGAATCGAGAAAACAGCTCCTCCAGTGGGGCGGCTTAGATGGATCAGCTTCGATATTCCAGTTGGATGTTCCCGCCACCATGCCGGTTGTGAAGGAGCTGTGTGGGCTGGAAACTGTGGAGAGGTACTTGGAATGGATCACTGGCAGAAACCAAGGTTCGCTCAACCGGCAAGGGGTTCGTCATTCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1381

Amino Acids

150.86

Weight (kDa)

6.09

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	17 - 84	1.3e-06	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	95 - 181	1.4e-20	[2Fe-2S] binding domain
FAD_binding_5	PF00941	241 - 423	2.3e-39	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	441 - 546	5.4e-20	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	615 - 724	2e-31	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	752 - 983	1.1e-83	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1013 - 1274	2.1e-72	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	789, 3795
Acc36I	ACCTGC	1 cut(s)	482
AccBSI	CCGCTC	1 cut(s)	696
AccI	GTMKAC	2 cut(s)	1863, 3008
AccII	CGCG	1 cut(s)	3870
AccIII	TCCGGA	1 cut(s)	670
AclI	AACGTT	2 cut(s)	1284, 3834
AclWI	GGATC	8 cut(s)	707, 720, 905, 1223, 1236, 1790, 4000, 4094
AcoI	YGGCCR	3 cut(s)	3, 1695, 3036
AcsI	RAATTY	6 cut(s)	1087, 1945, 3080, 3114, 3630, 3798
AcuI	CTGAAG	5 cut(s)	1035, 1104, 1407, 1827, 2901
AfeI	AGCGCT	2 cut(s)	854, 2989
AflII	CTTAAG	1 cut(s)	2768
AflIII	ACRYGT	2 cut(s)	2896, 3868
AhdI	GACNNNNNGTC	1 cut(s)	1873
AjnI	CCWGG	4 cut(s)	39, 376, 1519, 2851
AloI	GAACNNNNNNTCC	2 cut(s)	563, 595
Alw21I	GWGCWC	7 cut(s)	75, 406, 2554, 2791, 2985, 3374, 3876
Alw26I	GTCTC	7 cut(s)	248, 1224, 1835, 1881, 2345, 3291, 3674
AlwI	GGATC	8 cut(s)	707, 720, 905, 1223, 1236, 1790, 4000, 4094
AlwNI	CAGNNNCTG	1 cut(s)	779
Ama87I	CYCGRG	1 cut(s)	3120
Aor13HI	TCCGGA	1 cut(s)	670
Aor51HI	AGCGCT	2 cut(s)	854, 2989
AoxI	GGCC	7 cut(s)	3, 292, 1695, 2517, 2847, 3036, 3152
ApaI	GGGCCC	1 cut(s)	2851
ApoI	RAATTY	6 cut(s)	1087, 1945, 3080, 3114, 3630, 3798
AseI	ATTAAT	2 cut(s)	2705, 2943
Asp700I	GAANNNNTTC	1 cut(s)	1285
AspA2I	CCTAGG	1 cut(s)	1345
AspLEI	GCGC	3 cut(s)	855, 2990, 3584
AspS9I	GGNCC	4 cut(s)	211, 2233, 2847, 2848
AsuC2I	CCSGG	2 cut(s)	3367, 3677
AvaI	CYCGRG	1 cut(s)	3120
AvaII	GGWCC	2 cut(s)	211, 2233
AvrII	CCTAGG	1 cut(s)	1345
BaeGI	GKGCMC	2 cut(s)	2420, 2851
BalI	TGGCCA	1 cut(s)	3038
BamHI	GGATCC	2 cut(s)	712, 1228
BanII	GRGCYC	4 cut(s)	75, 2851, 3234, 3473
BarI	GAAGNNNNNNTAC	2 cut(s)	2633, 2665
BbsI	GAAGAC	3 cut(s)	2388, 2609, 3036
Bbv12I	GWGCWC	7 cut(s)	75, 406, 2554, 2791, 2985, 3374, 3876
BbvCI	CCTCAGC	1 cut(s)	1488
BccI	CCATC	9 cut(s)	1003, 1166, 2666, 3064, 3179, 3247, 3281, 3755, 3983
BcgI	CGANNNNNNTGC	6 cut(s)	188, 222, 529, 563, 4109, 4143
BciT130I	CCWGG	4 cut(s)	41, 378, 1521, 2853
BciVI	GTATCC	1 cut(s)	2259
BclI	TGATCA	1 cut(s)	2293
BcnI	CCSGG	2 cut(s)	3367, 3677
BcoDI	GTCTC	7 cut(s)	248, 1224, 1835, 1881, 2345, 3291, 3674
BfaI	CTAG	8 cut(s)	1074, 1179, 1346, 1382, 1929, 2690, 2726, 2792
BfmI	CTRYAG	3 cut(s)	21, 2286, 2916
BfoI	RGCGCY	3 cut(s)	856, 2991, 3585
BfrI	CTTAAG	1 cut(s)	2768
BfuAI	ACCTGC	1 cut(s)	482
BfuI	GTATCC	1 cut(s)	2259
BglII	AGATCT	3 cut(s)	1181, 2216, 2692
BlnI	CCTAGG	1 cut(s)	1345
BlpI	GCTNAGC	1 cut(s)	146
Bme1390I	CCNGG	6 cut(s)	41, 378, 1521, 2853, 3367, 3677
Bme18I	GGWCC	2 cut(s)	211, 2233
BmeRI	GACNNNNNGTC	1 cut(s)	1873
BmeT110I	CYCGRG	1 cut(s)	3120
BmgT120I	GGNCC	4 cut(s)	211, 2233, 2847, 2848
BmiI	GGNNCC	8 cut(s)	317, 714, 1230, 1566, 1721, 2235, 2849, 2850
BmrFI	CCNGG	6 cut(s)	41, 378, 1521, 2853, 3367, 3677
BmrI	ACTGGG	5 cut(s)	522, 1512, 2791, 3230, 3490
BmsI	GCATC	5 cut(s)	523, 2641, 2912, 3512, 3865
BmuI	ACTGGG	5 cut(s)	522, 1512, 2791, 3230, 3490
BpiI	GAAGAC	3 cut(s)	2388, 2609, 3036
BplI	GAGNNNNNCTC	2 cut(s)	3142, 3174
BpmI	CTGGAG	7 cut(s)	1275, 1845, 2643, 3023, 3144, 3639, 3956
Bpu10I	CCTNAGC	1 cut(s)	1488
Bpu1102I	GCTNAGC	1 cut(s)	146
BpuEI	CTTGAG	2 cut(s)	2717, 3428
BpuMI	CCSGG	2 cut(s)	3367, 3677
Bsa29I	ATCGAT	1 cut(s)	520
BsaAI	YACGTR	1 cut(s)	3786
BsaI	GGTCTC	2 cut(s)	1835, 3291
BsaJI	CCNNGG	9 cut(s)	295, 1345, 1723, 2452, 2851, 2852, 3059, 3507, 4102
BsaWI	WCCGGW	2 cut(s)	670, 1798
BsaXI	ACNNNNNCTCC	6 cut(s)	924, 954, 2809, 2839, 3954, 3984
Bse118I	RCCGGY	2 cut(s)	4033, 4116
BseAI	TCCGGA	1 cut(s)	670
BseBI	CCWGG	4 cut(s)	41, 378, 1521, 2853
BseCI	ATCGAT	1 cut(s)	520
BseDI	CCNNGG	9 cut(s)	295, 1345, 1723, 2452, 2851, 2852, 3059, 3507, 4102
BseGI	GGATG	8 cut(s)	284, 1177, 1963, 2592, 2632, 2903, 3503, 4021
BseRI	GAGGAG	3 cut(s)	92, 767, 3959
BseSI	GKGCMC	2 cut(s)	2420, 2851
BsgI	GTGCAG	5 cut(s)	1317, 1965, 2544, 2748, 2882
Bsh1236I	CGCG	1 cut(s)	3870
BshFI	GGCC	7 cut(s)	5, 294, 1697, 2519, 2849, 3038, 3154
BshVI	ATCGAT	1 cut(s)	520
BsiHKAI	GWGCWC	7 cut(s)	75, 406, 2554, 2791, 2985, 3374, 3876
BsiHKCI	CYCGRG	1 cut(s)	3120
BsiSI	CCGG	6 cut(s)	671, 1799, 3366, 3677, 4034, 4117
BslFI	GGGAC	4 cut(s)	841, 2219, 3041, 3530
BsmAI	GTCTC	7 cut(s)	248, 1224, 1835, 1881, 2345, 3291, 3674
BsmBI	CGTCTC	1 cut(s)	1881
BsmFI	GGGAC	4 cut(s)	841, 2219, 3041, 3530
BsnI	GGCC	7 cut(s)	5, 294, 1697, 2519, 2849, 3038, 3154
Bso31I	GGTCTC	2 cut(s)	1835, 3291
BsoBI	CYCGRG	1 cut(s)	3120
Bsp120I	GGGCCC	1 cut(s)	2847
Bsp13I	TCCGGA	1 cut(s)	670
Bsp1407I	TGTACA	1 cut(s)	662
Bsp1720I	GCTNAGC	1 cut(s)	146
BspANI	GGCC	7 cut(s)	5, 294, 1697, 2519, 2849, 3038, 3154
BspDI	ATCGAT	1 cut(s)	520
BspEI	TCCGGA	1 cut(s)	670
BspFNI	CGCG	1 cut(s)	3870
BspLI	GGNNCC	8 cut(s)	317, 714, 1230, 1566, 1721, 2235, 2849, 2850
BspMAI	CTGCAG	2 cut(s)	25, 2290
BspMI	ACCTGC	1 cut(s)	482
BspPI	GGATC	8 cut(s)	707, 720, 905, 1223, 1236, 1790, 4000, 4094
BspQI	GCTCTTC	1 cut(s)	703
BspTI	CTTAAG	1 cut(s)	2768
BspTNI	GGTCTC	2 cut(s)	1835, 3291
BsrBI	CCGCTC	1 cut(s)	696
BsrFI	RCCGGY	2 cut(s)	4033, 4116
BsrGI	TGTACA	1 cut(s)	662
BssAI	RCCGGY	2 cut(s)	4033, 4116
BssECI	CCNNGG	9 cut(s)	295, 1345, 1723, 2452, 2851, 2852, 3059, 3507, 4102
BssNAI	GTATAC	1 cut(s)	3009
BssT1I	CCWWGG	7 cut(s)	295, 1345, 1723, 2452, 3059, 3507, 4102
Bst1107I	GTATAC	1 cut(s)	3009
Bst2UI	CCWGG	4 cut(s)	41, 378, 1521, 2853
Bst6I	CTCTTC	5 cut(s)	703, 1931, 2196, 2440, 3883
BstAFI	CTTAAG	1 cut(s)	2768
BstAPI	GCANNNNNTGC	1 cut(s)	3263
BstAUI	TGTACA	1 cut(s)	662
BstBAI	YACGTR	1 cut(s)	3786
BstC8I	GCNNGC	5 cut(s)	538, 2847, 3388, 3501, 3872
BstEII	GGTNACC	1 cut(s)	494
BstF5I	GGATG	8 cut(s)	284, 1177, 1963, 2592, 2632, 2903, 3503, 4021
BstFNI	CGCG	1 cut(s)	3870
BstH2I	RGCGCY	3 cut(s)	856, 2991, 3585
BstHHI	GCGC	3 cut(s)	855, 2990, 3584
BstMAI	GTCTC	7 cut(s)	248, 1224, 1835, 1881, 2345, 3291, 3674
BstNI	CCWGG	4 cut(s)	41, 378, 1521, 2853
BstNSI	RCATGY	3 cut(s)	385, 2129, 2900
BstPI	GGTNACC	1 cut(s)	494
BstSCI	CCNGG	6 cut(s)	39, 376, 1519, 2851, 3365, 3675
BstSFI	CTRYAG	3 cut(s)	21, 2286, 2916
BstSLI	GKGCMC	2 cut(s)	2420, 2851
BstUI	CGCG	1 cut(s)	3870
BstV2I	GAAGAC	3 cut(s)	2388, 2609, 3036
BstX2I	RGATCY	6 cut(s)	712, 910, 1181, 1228, 2216, 2692
BstXI	CCANNNNNNTGG	2 cut(s)	1036, 2536
BstYI	RGATCY	6 cut(s)	712, 910, 1181, 1228, 2216, 2692
BstZ17I	GTATAC	1 cut(s)	3009
Bsu15I	ATCGAT	1 cut(s)	520
BsuI	GTATCC	1 cut(s)	2259
BsuRI	GGCC	7 cut(s)	5, 294, 1697, 2519, 2849, 3038, 3154
BsuTUI	ATCGAT	1 cut(s)	520
BtsCI	GGATG	8 cut(s)	284, 1177, 1963, 2592, 2632, 2903, 3503, 4021
BtsI	GCAGTG	1 cut(s)	265
BveI	ACCTGC	1 cut(s)	482
Cac8I	GCNNGC	5 cut(s)	538, 2847, 3388, 3501, 3872
CaiI	CAGNNNCTG	1 cut(s)	779
CfoI	GCGC	3 cut(s)	855, 2990, 3584
Cfr10I	RCCGGY	2 cut(s)	4033, 4116
Cfr13I	GGNCC	4 cut(s)	211, 2233, 2847, 2848
ClaI	ATCGAT	1 cut(s)	520
CseI	GACGC	2 cut(s)	1544, 2081
CspCI	CAANNNNNGTGG	2 cut(s)	2130, 2165
DraI	TTTAAA	2 cut(s)	1368, 1996
DriI	GACNNNNNGTC	1 cut(s)	1873
EaeI	YGGCCR	3 cut(s)	3, 1695, 3036
Eam1104I	CTCTTC	5 cut(s)	703, 1931, 2196, 2440, 3883
Eam1105I	GACNNNNNGTC	1 cut(s)	1873
EarI	CTCTTC	5 cut(s)	703, 1931, 2196, 2440, 3883
Ecl136II	GAGCTC	1 cut(s)	73
Eco130I	CCWWGG	7 cut(s)	295, 1345, 1723, 2452, 3059, 3507, 4102
Eco147I	AGGCCT	1 cut(s)	294
Eco24I	GRGCYC	4 cut(s)	75, 2851, 3234, 3473
Eco31I	GGTCTC	2 cut(s)	1835, 3291
Eco47I	GGWCC	2 cut(s)	211, 2233
Eco47III	AGCGCT	2 cut(s)	854, 2989
Eco53kI	GAGCTC	1 cut(s)	73
Eco57I	CTGAAG	5 cut(s)	1035, 1104, 1407, 1827, 2901
Eco88I	CYCGRG	1 cut(s)	3120
Eco91I	GGTNACC	1 cut(s)	494
EcoICRI	GAGCTC	1 cut(s)	73
EcoO109I	RGGNCCY	2 cut(s)	2233, 2848
EcoO65I	GGTNACC	1 cut(s)	494
EcoRI	GAATTC	1 cut(s)	3630
EcoRII	CCWGG	4 cut(s)	39, 376, 1519, 2851
EcoT14I	CCWWGG	7 cut(s)	295, 1345, 1723, 2452, 3059, 3507, 4102
EcoT38I	GRGCYC	4 cut(s)	75, 2851, 3234, 3473
ErhI	CCWWGG	7 cut(s)	295, 1345, 1723, 2452, 3059, 3507, 4102
Esp3I	CGTCTC	1 cut(s)	1881
FalI	AAGNNNNNCTT	2 cut(s)	1576, 1608
FaqI	GGGAC	4 cut(s)	841, 2219, 3041, 3530
FauI	CCCGC	2 cut(s)	2838, 4030
FauNDI	CATATG	1 cut(s)	1035
FbaI	TGATCA	1 cut(s)	2293
FblI	GTMKAC	2 cut(s)	1863, 3008
FokI	GGATG	8 cut(s)	271, 1184, 1950, 2599, 2619, 2890, 3490, 4028
FriOI	GRGCYC	4 cut(s)	75, 2851, 3234, 3473
FspBI	CTAG	8 cut(s)	1074, 1179, 1346, 1382, 1929, 2690, 2726, 2792
GlaI	GCGC	3 cut(s)	854, 2989, 3583
GsuI	CTGGAG	7 cut(s)	1275, 1845, 2643, 3023, 3144, 3639, 3956
HaeII	RGCGCY	3 cut(s)	856, 2991, 3585
HaeIII	GGCC	7 cut(s)	5, 294, 1697, 2519, 2849, 3038, 3154
HapII	CCGG	6 cut(s)	671, 1799, 3366, 3677, 4034, 4117
HgaI	GACGC	2 cut(s)	1544, 2081
HhaI	GCGC	3 cut(s)	855, 2990, 3584
Hin6I	GCGC	3 cut(s)	853, 2988, 3582
HinP1I	GCGC	3 cut(s)	853, 2988, 3582
HincII	GTYRAC	2 cut(s)	55, 3478
HindII	GTYRAC	2 cut(s)	55, 3478
HindIII	AAGCTT	5 cut(s)	963, 1603, 1839, 3049, 3885
HinfI	GANTC	8 cut(s)	989, 1151, 1516, 1700, 2661, 2927, 3667, 3953
HpaI	GTTAAC	2 cut(s)	55, 3478
HpaII	CCGG	6 cut(s)	671, 1799, 3366, 3677, 4034, 4117
Hpy99I	CGWCG	1 cut(s)	11
HpyCH4IV	ACGT	3 cut(s)	1284, 3785, 3834
HpySE526I	ACGT	3 cut(s)	1284, 3785, 3834
HspAI	GCGC	3 cut(s)	853, 2988, 3582
Kpn2I	TCCGGA	1 cut(s)	670
Ksp22I	TGATCA	1 cut(s)	2293
KspAI	GTTAAC	2 cut(s)	55, 3478
LguI	GCTCTTC	1 cut(s)	703
LmnI	GCTCC	8 cut(s)	754, 844, 932, 1898, 3369, 3460, 3972, 4045
LweI	GCATC	5 cut(s)	523, 2641, 2912, 3512, 3865
MaeI	CTAG	8 cut(s)	1074, 1179, 1346, 1382, 1929, 2690, 2726, 2792
MaeII	ACGT	3 cut(s)	1284, 3785, 3834
MaeIII	GTNAC	4 cut(s)	119, 494, 940, 2264
MbiI	CCGCTC	1 cut(s)	696
MfeI	CAATTG	3 cut(s)	525, 936, 2439
MflI	RGATCY	6 cut(s)	712, 910, 1181, 1228, 2216, 2692
MlsI	TGGCCA	1 cut(s)	3038
MluI	ACGCGT	1 cut(s)	3868
MluNI	TGGCCA	1 cut(s)	3038
MlyI	GAGTC	2 cut(s)	1525, 3676
MmeI	TCCRAC	6 cut(s)	796, 938, 1179, 2476, 3187, 3993
Mox20I	TGGCCA	1 cut(s)	3038
MroI	TCCGGA	1 cut(s)	670
MroXI	GAANNNNTTC	1 cut(s)	1285
MscI	TGGCCA	1 cut(s)	3038
MslI	CAYNNNNRTG	2 cut(s)	651, 2534
Msp20I	TGGCCA	1 cut(s)	3038
MspA1I	CMGCKG	3 cut(s)	150, 554, 2291
MspCI	CTTAAG	1 cut(s)	2768
MspI	CCGG	6 cut(s)	671, 1799, 3366, 3677, 4034, 4117
MspR9I	CCNGG	6 cut(s)	41, 378, 1521, 2853, 3367, 3677
MunI	CAATTG	3 cut(s)	525, 936, 2439
MvaI	CCWGG	4 cut(s)	41, 378, 1521, 2853
MvnI	CGCG	1 cut(s)	3870
NciI	CCSGG	2 cut(s)	3367, 3677
NdeI	CATATG	1 cut(s)	1035
NlaIV	GGNNCC	8 cut(s)	317, 714, 1230, 1566, 1721, 2235, 2849, 2850
NmuCI	GTSAC	2 cut(s)	119, 2264
NspI	RCATGY	3 cut(s)	385, 2129, 2900
PaeR7I	CTCGAG	1 cut(s)	3120
PasI	CCCWGGG	1 cut(s)	2852
PceI	AGGCCT	1 cut(s)	294
PciI	ACATGT	1 cut(s)	2896
PciSI	GCTCTTC	1 cut(s)	703
PcsI	WCGNNNNNNNCGW	2 cut(s)	2073, 2109
PdmI	GAANNNNTTC	1 cut(s)	1285
PfeI	GAWTC	6 cut(s)	989, 1151, 1700, 2661, 2927, 3953
PfoI	TCCNGGA	1 cut(s)	3365
PleI	GAGTC	2 cut(s)	1524, 3675
PpsI	GAGTC	2 cut(s)	1524, 3675
Ppu21I	YACGTR	1 cut(s)	3786
PpuMI	RGGWCCY	1 cut(s)	2233
PscI	ACATGT	1 cut(s)	2896
PshBI	ATTAAT	2 cut(s)	2705, 2943
PsiI	TTATAA	2 cut(s)	789, 3795
Psp124BI	GAGCTC	1 cut(s)	75
Psp1406I	AACGTT	2 cut(s)	1284, 3834
Psp5II	RGGWCCY	1 cut(s)	2233
Psp6I	CCWGG	4 cut(s)	39, 376, 1519, 2851
PspEI	GGTNACC	1 cut(s)	494
PspGI	CCWGG	4 cut(s)	39, 376, 1519, 2851
PspN4I	GGNNCC	8 cut(s)	317, 714, 1230, 1566, 1721, 2235, 2849, 2850
PspOMI	GGGCCC	1 cut(s)	2847
PspPI	GGNCC	4 cut(s)	211, 2233, 2847, 2848
PspPPI	RGGWCCY	1 cut(s)	2233
PstI	CTGCAG	2 cut(s)	25, 2290
PstNI	CAGNNNCTG	1 cut(s)	779
PsuI	RGATCY	6 cut(s)	712, 910, 1181, 1228, 2216, 2692
PvuII	CAGCTG	3 cut(s)	150, 554, 2291
RseI	CAYNNNNRTG	2 cut(s)	651, 2534
SacI	GAGCTC	1 cut(s)	75
SapI	GCTCTTC	1 cut(s)	703
Sau96I	GGNCC	4 cut(s)	211, 2233, 2847, 2848
SchI	GAGTC	2 cut(s)	1525, 3676
ScrFI	CCNGG	6 cut(s)	41, 378, 1521, 2853, 3367, 3677
SfaNI	GCATC	5 cut(s)	523, 2641, 2912, 3512, 3865
SfcI	CTRYAG	3 cut(s)	21, 2286, 2916
Sfr274I	CTCGAG	1 cut(s)	3120
SinI	GGWCC	2 cut(s)	211, 2233
SlaI	CTCGAG	1 cut(s)	3120
SmiI	ATTTAAAT	1 cut(s)	1368
SmiMI	CAYNNNNRTG	2 cut(s)	651, 2534
SmlI	CTYRAG	4 cut(s)	2696, 2768, 3120, 3443
SmoI	CTYRAG	4 cut(s)	2696, 2768, 3120, 3443
SseBI	AGGCCT	1 cut(s)	294
SspI	AATATT	2 cut(s)	2755, 3418
SspMI	CTAG	8 cut(s)	1074, 1179, 1346, 1382, 1929, 2690, 2726, 2792
SstI	GAGCTC	1 cut(s)	75
StuI	AGGCCT	1 cut(s)	294
StyD4I	CCNGG	6 cut(s)	39, 376, 1519, 2851, 3365, 3675
StyI	CCWWGG	7 cut(s)	295, 1345, 1723, 2452, 3059, 3507, 4102
SwaI	ATTTAAAT	1 cut(s)	1368
TaiI	ACGT	3 cut(s)	1287, 3788, 3837
TatI	WGTACW	6 cut(s)	371, 509, 662, 1240, 1784, 1913
TauI	GCSGC	2 cut(s)	156, 3984
TfiI	GAWTC	6 cut(s)	989, 1151, 1700, 2661, 2927, 3953
TseFI	GTSAC	2 cut(s)	119, 2264
Tsp45I	GTSAC	2 cut(s)	119, 2264
TspGWI	ACGGA	1 cut(s)	1863
Vha464I	CTTAAG	1 cut(s)	2768
VpaK11BI	GGWCC	2 cut(s)	211, 2233
VspI	ATTAAT	2 cut(s)	2705, 2943
XapI	RAATTY	6 cut(s)	1087, 1945, 3080, 3114, 3630, 3798
XbaI	TCTAGA	2 cut(s)	1178, 2689
XceI	RCATGY	3 cut(s)	385, 2129, 2900
XcmI	CCANNNNNNNNNTGG	3 cut(s)	338, 1417, 1675
XhoI	CTCGAG	1 cut(s)	3120
XmaJI	CCTAGG	1 cut(s)	1345
XmiI	GTMKAC	2 cut(s)	1863, 3008
XmnI	GAANNNNTTC	1 cut(s)	1285
XspI	CTAG	8 cut(s)	1074, 1179, 1346, 1382, 1929, 2690, 2726, 2792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.