pycom03g11540

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010040
Physical Location & Seq
Forward (+)
11354539 .. 11355578
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGGAGGTAAAGCCGAATTCTGCAACTATTAATCGTGTTTACGAAGACATTAAACCATCGGAAGAATGGGCAAGGGAGGAAGCTTGTGACACTCTCCTCGTCTATCTGCAGGGATTTAGAAAGGAGAATCTGAGGATTCAATTGACGTTAGCTCAAAACTTAAGGGTGCTTGGTGAACGGCCACTTGTTCATGACAACAAATGGCAGCGTTTCTACATGGAATTCCCAATTCCCTCCAATTGCGACACCAATGCAATCTCTGCCAAATTTGAGAATGACATTCTCTATGTCAAGCTTCCAAAGCTGATTGATCTCGCAGCAGTTAAATCGAATGAGAAACCACAAAAGCCACCCACCACCACAGAAGCACCTAAGCCTCAAAAGCACACACCCACCACAGAAGCACCTAAGCCTCAAAAGCCCATAAGCACTGGTGATCTGCCACATCCTCAAAAGAGGACTCATGCTAATCAAGAACAGAACAAGAAACACAATGGTGGATCACACGTGAACAAAAGTGCTGAAAAACTTGAGGAGATTCGTGCGAAAAAGGCCGAGGAGGATTCAACAAAGGAGGTATACAAAAGAGCAATATTCAGGGATGAGAGGAATGCTGGCGGTGTTGATAGGAATTATAGAGCACAAGGTTATAACTATAAGCAGGTGTTTCAAGGCTTGGTCATGGCATTTGTATTTATTCTGGTACTTGGGTTGTATGTCAAGCATGTATCAAGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

247

Amino Acids

28.23

Weight (kDa)

9.3

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 34 - 103 9e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AarI CACCTGC 1 cut(s) 652
Acc36I ACCTGC 1 cut(s) 652
AccI GTMKAC 1 cut(s) 579
AciI CCGC 1 cut(s) 618
AclWI GGATC 1 cut(s) 508
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 3 cut(s) 16, 221, 266
AcvI CACGTG 1 cut(s) 508
AfaI GTAC 1 cut(s) 705
AflII CTTAAG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 3 cut(s) 140, 567, 671
AleI CACNNNNGTG 1 cut(s) 495
AluBI AGCT 4 cut(s) 83, 152, 295, 304
AluI AGCT 4 cut(s) 83, 152, 295, 304
Alw21I GWGCWC 1 cut(s) 643
AlwI GGATC 1 cut(s) 508
AoxI GGCC 2 cut(s) 179, 552
ApeKI GCWGC 2 cut(s) 205, 317
ApoI RAATTY 3 cut(s) 16, 221, 266
AseI ATTAAT 1 cut(s) 30
AsuHPI GGTGA 2 cut(s) 185, 446
BaeI ACNNNNGTAYC 2 cut(s) 695, 728
BbrPI CACGTG 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 2 cut(s) 217, 329
BccI CCATC 1 cut(s) 64
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 309, 343
BfmI CTRYAG 1 cut(s) 107
BfrI CTTAAG 1 cut(s) 160
BfuAI ACCTGC 1 cut(s) 652
BisI GCNGC 2 cut(s) 206, 318
BlsI GCNGC 2 cut(s) 207, 319
BpiI GAAGAC 1 cut(s) 51
Bpu10I CCTNAGC 2 cut(s) 372, 408
BpuEI CTTGAG 1 cut(s) 551
BsaAI YACGTR 1 cut(s) 508
BsaJI CCNNGG 1 cut(s) 555
Bse1I ACTGG 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 555
BseGI GGATG 2 cut(s) 445, 607
BseMII CTCAG 1 cut(s) 122
BseNI ACTGG 1 cut(s) 436
BseRI GAGGAG 3 cut(s) 86, 548, 572
BseXI GCAGC 2 cut(s) 217, 329
BshFI GGCC 2 cut(s) 181, 554
BsiHKAI GWGCWC 1 cut(s) 643
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 2 cut(s) 181, 554
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 3 cut(s) 310, 436, 500
BspACI CCGC 1 cut(s) 618
BspANI GGCC 2 cut(s) 181, 554
BspCNI CTCAG 1 cut(s) 123
BspHI TCATGA 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 1 cut(s) 508
BspTI CTTAAG 1 cut(s) 160
BsrI ACTGG 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 3 cut(s) 310, 436, 500
BssNAI GTATAC 1 cut(s) 580
Bst1107I GTATAC 1 cut(s) 580
BstAFI CTTAAG 1 cut(s) 160
BstAPI GCANNNNNTGC 1 cut(s) 260
BstBAI YACGTR 1 cut(s) 508
BstC8I GCNNGC 1 cut(s) 616
BstDEI CTNAG 3 cut(s) 131, 372, 408
BstF5I GGATG 2 cut(s) 445, 607
BstKTI GATC 3 cut(s) 313, 439, 503
BstMBI GATC 3 cut(s) 310, 436, 500
BstMWI GCNNNNNNNGC 5 cut(s) 260, 301, 382, 418, 551
BstNSI RCATGY 2 cut(s) 728, 739
BstSFI CTRYAG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 217, 329
BstV2I GAAGAC 1 cut(s) 51
BstZ17I GTATAC 1 cut(s) 580
BsuRI GGCC 2 cut(s) 181, 554
BtsCI GGATG 2 cut(s) 445, 607
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 616
CciI TCATGA 1 cut(s) 190
Csp6I GTAC 1 cut(s) 704
CviAII CATG 6 cut(s) 191, 217, 464, 682, 725, 736
CviQI GTAC 1 cut(s) 704
DdeI CTNAG 3 cut(s) 131, 372, 408
DpnI GATC 3 cut(s) 312, 438, 502
DpnII GATC 3 cut(s) 310, 436, 500
EaeI YGGCCR 1 cut(s) 179
Eco72I CACGTG 1 cut(s) 508
EcoRI GAATTC 2 cut(s) 16, 221
FaeI CATG 6 cut(s) 194, 220, 467, 685, 728, 739
FatI CATG 6 cut(s) 190, 216, 463, 681, 724, 735
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 206, 318
FokI GGATG 2 cut(s) 432, 614
Fsp4HI GCNGC 2 cut(s) 206, 318
GluI GCNGC 2 cut(s) 206, 318
HaeIII GGCC 2 cut(s) 181, 554
Hin1II CATG 6 cut(s) 194, 220, 467, 685, 728, 739
HindIII AAGCTT 2 cut(s) 81, 293
HinfI GANTC 5 cut(s) 127, 136, 460, 538, 563
HphI GGTGA 2 cut(s) 185, 446
Hpy166II GTNNAC 4 cut(s) 40, 176, 511, 580
Hpy188I TCNGA 2 cut(s) 61, 132
Hpy188III TCNNGA 2 cut(s) 191, 473
Hpy8I GTNNAC 4 cut(s) 40, 176, 511, 580
HpyCH4IV ACGT 2 cut(s) 146, 507
HpyCH4V TGCA 3 cut(s) 23, 109, 254
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 301, 382, 418, 551
HpyF3I CTNAG 3 cut(s) 131, 372, 408
HpySE526I ACGT 2 cut(s) 146, 507
Hsp92II CATG 6 cut(s) 194, 220, 467, 685, 728, 739
Kzo9I GATC 3 cut(s) 310, 436, 500
LpnPI CCDG 6 cut(s) 95, 417, 583, 600, 647, 686
Lsp1109I GCAGC 2 cut(s) 217, 329
MaeII ACGT 2 cut(s) 146, 507
MaeIII GTNAC 1 cut(s) 86
MalI GATC 3 cut(s) 312, 438, 502
MboI GATC 3 cut(s) 310, 436, 500
MboII GAAGA 2 cut(s) 56, 74
MfeI CAATTG 2 cut(s) 140, 238
MhlI GDGCHC 1 cut(s) 643
MluCI AATT 7 cut(s) 16, 140, 221, 228, 238, 266, 631
MlyI GAGTC 1 cut(s) 454
MseI TTAA 4 cut(s) 30, 51, 161, 324
MslI CAYNNNNRTG 1 cut(s) 495
MspCI CTTAAG 1 cut(s) 160
MunI CAATTG 2 cut(s) 140, 238
Mva1269I GAATGC 1 cut(s) 616
MwoI GCNNNNNNNGC 5 cut(s) 260, 301, 382, 418, 551
NdeII GATC 3 cut(s) 310, 436, 500
NlaIII CATG 6 cut(s) 194, 220, 467, 685, 728, 739
NmeAIII GCCGAG 1 cut(s) 580
NmuCI GTSAC 1 cut(s) 86
NspI RCATGY 2 cut(s) 728, 739
OliI CACNNNNGTG 1 cut(s) 495
PagI TCATGA 1 cut(s) 190
PaqCI CACCTGC 1 cut(s) 652
PctI GAATGC 1 cut(s) 616
PfeI GAWTC 4 cut(s) 127, 136, 538, 563
PkrI GCNGC 2 cut(s) 207, 319
PleI GAGTC 1 cut(s) 454
PmaCI CACGTG 1 cut(s) 508
PmlI CACGTG 1 cut(s) 508
PpsI GAGTC 1 cut(s) 454
Ppu21I YACGTR 1 cut(s) 508
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 651
PspCI CACGTG 1 cut(s) 508
PstI CTGCAG 1 cut(s) 111
RsaI GTAC 1 cut(s) 705
RsaNI GTAC 1 cut(s) 704
RseI CAYNNNNRTG 1 cut(s) 495
SaqAI TTAA 4 cut(s) 30, 51, 161, 324
SatI GCNGC 2 cut(s) 206, 318
Sau3AI GATC 3 cut(s) 310, 436, 500
SchI GAGTC 1 cut(s) 454
SduI GDGCHC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 2 cut(s) 160, 530
SmoI CTYRAG 2 cut(s) 160, 530
Sse9I AATT 7 cut(s) 16, 140, 221, 228, 238, 266, 631
SsiI CCGC 1 cut(s) 618
SspI AATATT 1 cut(s) 594
TaiI ACGT 2 cut(s) 149, 510
TaqI TCGA 1 cut(s) 329
TasI AATT 7 cut(s) 16, 140, 221, 228, 238, 266, 631
TfiI GAWTC 4 cut(s) 127, 136, 538, 563
Tru1I TTAA 4 cut(s) 30, 51, 161, 324
Tru9I TTAA 4 cut(s) 30, 51, 161, 324
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 2 cut(s) 205, 317
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 1 cut(s) 436
Vha464I CTTAAG 1 cut(s) 160
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 3 cut(s) 16, 221, 266
XceI RCATGY 2 cut(s) 728, 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.