pycom03g12220

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007205
Physical Location & Seq
Reverse (-)
12607050 .. 12609947
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGACTTCGGTCATCAACATGGACGGTCATGATCAGCTACTCACCGATGATCTTACCGATTTATCCACCCTGCAACATCATCACGATCAGGGGGAAATGCAGAGCAAAGAGGAAAATGTTGAAAAGAAATGTGGGAATCATGATGGGGTTTGTGCAATATGCTTGGATGAGATTGTGCTTCAAGACACCGCTCTGATAAAAGGTTGCGAGCATGCCTACTGCGCAACCTGTATTCTTCGTTGGGTGACATACAGCCAAAAACCCACCTGCCCTCAGTGTAAACATCCATTTGAATTCCTGAACGTCCATCGTTCCCTTGATGGCAGCATACAAGATTACATGTTCGAGGAGAGTGTGTGCCTACTCCTTAGAGCCAAATGGTTTGAGCCGTTAGTTGTGGAGGAGCGTGAAGATGTGTATGATGGCCATGAGGATTATTATTACTATCCCTATGAGGATGAAGAGGATGATGATCTAGACGACGCTTACTTCAGCAGTTCATCGGTTATCCGGATTGGTAATCGAAGATGGGGAGATAATGGATACGTGAGGGGTGGGCGTCAAGAAGCAAGGCCTGTACGTAGATCAAACATACAGGACCCGGGTGCTAGTTCGTCTTGTGAGCCTAGGAAGAAAGAGGCTGCAGTGGATAAAACCGGGAGAAGGGCAAAGCGGGCGCTCAAACGGGAAGCGGCTGACAAGGCAGCCGCAGAGAAACATCAGCAGCATTTGGCGAGGTTGGGTCGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

251

Amino Acids

28.69

Weight (kDa)

5.39

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 48 - 98 6.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 51 - 94 7.2e-10 Ring finger domain
zf-C3HC4_2 PF13923 51 - 93 7.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 51 - 93 8.8e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 275
Acc16I TGCGCA 1 cut(s) 223
Acc36I ACCTGC 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 191
AccIII TCCGGA 1 cut(s) 510
AciI CCGC 4 cut(s) 189, 673, 692, 708
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 1 cut(s) 579
AfiI CCNNNNNNNGG 1 cut(s) 663
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 3 cut(s) 122, 182, 293
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 601
Aor13HI TCCGGA 1 cut(s) 510
AoxI GGCC 2 cut(s) 424, 572
ApeKI GCWGC 4 cut(s) 324, 641, 704, 724
ApoI RAATTY 1 cut(s) 293
AspA2I CCTAGG 1 cut(s) 626
AspLEI GCGC 2 cut(s) 224, 679
AspS9I GGNCC 1 cut(s) 598
AsuC2I CCSGG 3 cut(s) 602, 603, 658
AsuHPI GGTGA 2 cut(s) 34, 256
AvaI CYCGRG 1 cut(s) 601
AvaII GGWCC 1 cut(s) 598
AvrII CCTAGG 1 cut(s) 626
BalI TGGCCA 1 cut(s) 426
BbvI GCAGC 4 cut(s) 336, 628, 716, 736
BccI CCATC 5 cut(s) 137, 314, 315, 416, 522
BceAI ACGGC 1 cut(s) 373
BciVI GTATCC 1 cut(s) 536
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 3 cut(s) 602, 603, 658
BfaI CTAG 3 cut(s) 476, 609, 627
BfmI CTRYAG 1 cut(s) 642
BfoI RGCGCY 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 275
BfuI GTATCC 1 cut(s) 536
BisI GCNGC 6 cut(s) 325, 642, 693, 705, 708, 725
BlnI CCTAGG 1 cut(s) 626
BlsI GCNGC 6 cut(s) 326, 643, 694, 706, 709, 726
Bme1390I CCNGG 3 cut(s) 602, 603, 658
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 1 cut(s) 600
BmrFI CCNGG 3 cut(s) 602, 603, 658
BoxI GACNNNNGTC 1 cut(s) 8
BpuMI CCSGG 3 cut(s) 602, 603, 658
BsaAI YACGTR 2 cut(s) 547, 581
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 559
BsaJI CCNNGG 2 cut(s) 601, 626
BsaWI WCCGGW 1 cut(s) 510
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 601, 626
BseGI GGATG 4 cut(s) 172, 283, 463, 472
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 663
BseMII CTCAG 1 cut(s) 287
BseRI GAGGAG 2 cut(s) 362, 416
BseXI GCAGC 4 cut(s) 336, 628, 716, 736
BshFI GGCC 2 cut(s) 426, 574
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 3 cut(s) 511, 602, 657
BslI CCNNNNNNNGG 1 cut(s) 663
BsnI GGCC 2 cut(s) 426, 574
BsoBI CYCGRG 1 cut(s) 601
Bsp13I TCCGGA 1 cut(s) 510
Bsp143I GATC 5 cut(s) 31, 49, 85, 472, 584
BspACI CCGC 4 cut(s) 189, 673, 692, 708
BspANI GGCC 2 cut(s) 426, 574
BspCNI CTCAG 1 cut(s) 286
BspEI TCCGGA 1 cut(s) 510
BspHI TCATGA 2 cut(s) 28, 139
BspLI GGNNCC 1 cut(s) 600
BspMAI CTGCAG 1 cut(s) 646
BspMI ACCTGC 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 601, 626
BssMI GATC 5 cut(s) 31, 49, 85, 472, 584
BssNI GRCGYC 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 626
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 559
BstBAI YACGTR 2 cut(s) 547, 581
BstC8I GCNNGC 3 cut(s) 209, 213, 675
BstDEI CTNAG 2 cut(s) 273, 368
BstF5I GGATG 4 cut(s) 172, 283, 463, 472
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 2 cut(s) 224, 679
BstKTI GATC 5 cut(s) 34, 52, 88, 475, 587
BstMBI GATC 5 cut(s) 31, 49, 85, 472, 584
BstMWI GCNNNNNNNGC 3 cut(s) 221, 674, 701
BstNSI RCATGY 2 cut(s) 215, 343
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 3 cut(s) 600, 601, 656
BstSFI CTRYAG 1 cut(s) 642
BstSNI TACGTA 1 cut(s) 581
BstV1I GCAGC 4 cut(s) 336, 628, 716, 736
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 426, 574
BtsCI GGATG 4 cut(s) 172, 283, 463, 472
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 281, 651
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 3 cut(s) 209, 213, 675
CciI TCATGA 2 cut(s) 28, 139
CfoI GCGC 2 cut(s) 224, 679
Cfr13I GGNCC 1 cut(s) 598
Cfr9I CCCGGG 1 cut(s) 601
CseI GACGC 2 cut(s) 491, 548
Csp6I GTAC 1 cut(s) 578
CviAII CATG 6 cut(s) 19, 29, 140, 212, 340, 428
CviQI GTAC 1 cut(s) 578
DdeI CTNAG 2 cut(s) 273, 368
DpnI GATC 5 cut(s) 33, 51, 87, 474, 586
DpnII GATC 5 cut(s) 31, 49, 85, 472, 584
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco105I TACGTA 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 626
Eco147I AGGCCT 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 293
EcoT14I CCWWGG 1 cut(s) 626
ErhI CCWWGG 1 cut(s) 626
FaeI CATG 6 cut(s) 22, 32, 143, 215, 343, 431
FatI CATG 6 cut(s) 18, 28, 139, 211, 339, 427
FauI CCCGC 1 cut(s) 666
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 6 cut(s) 325, 642, 693, 705, 708, 725
FokI GGATG 4 cut(s) 179, 270, 470, 479
Fsp4HI GCNGC 6 cut(s) 325, 642, 693, 705, 708, 725
FspBI CTAG 3 cut(s) 476, 609, 627
FspI TGCGCA 1 cut(s) 223
GlaI GCGC 2 cut(s) 223, 678
GluI GCNGC 6 cut(s) 325, 642, 693, 705, 708, 725
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 2 cut(s) 426, 574
HapII CCGG 3 cut(s) 511, 602, 657
HgaI GACGC 2 cut(s) 491, 548
HhaI GCGC 2 cut(s) 224, 679
Hin1I GRCGYC 1 cut(s) 559
Hin1II CATG 6 cut(s) 22, 32, 143, 215, 343, 431
Hin6I GCGC 2 cut(s) 222, 677
HinP1I GCGC 2 cut(s) 222, 677
HinfI GANTC 1 cut(s) 136
HpaII CCGG 3 cut(s) 511, 602, 657
HphI GGTGA 2 cut(s) 34, 256
Hpy166II GTNNAC 1 cut(s) 281
Hpy188I TCNGA 2 cut(s) 195, 747
Hpy188III TCNNGA 8 cut(s) 29, 83, 140, 182, 298, 476, 511, 563
Hpy8I GTNNAC 1 cut(s) 281
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 1 cut(s) 657
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 3 cut(s) 303, 546, 580
HpyCH4V TGCA 4 cut(s) 73, 100, 155, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 674, 701
HpyF3I CTNAG 2 cut(s) 273, 368
HpySE526I ACGT 3 cut(s) 303, 546, 580
Hsp92I GRCGYC 1 cut(s) 559
Hsp92II CATG 6 cut(s) 22, 32, 143, 215, 343, 431
HspAI GCGC 2 cut(s) 222, 677
Kpn2I TCCGGA 1 cut(s) 510
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 5 cut(s) 31, 49, 85, 472, 584
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 4 cut(s) 336, 628, 716, 736
MaeI CTAG 3 cut(s) 476, 609, 627
MaeII ACGT 3 cut(s) 303, 546, 580
MaeIII GTNAC 1 cut(s) 244
MalI GATC 5 cut(s) 33, 51, 87, 474, 586
MbiI CCGCTC 1 cut(s) 191
MboI GATC 5 cut(s) 31, 49, 85, 472, 584
MboII GAAGA 5 cut(s) 227, 422, 473, 537, 643
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 725
Mox20I TGGCCA 1 cut(s) 426
MroI TCCGGA 1 cut(s) 510
MscI TGGCCA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 426
MspI CCGG 3 cut(s) 511, 602, 657
MspR9I CCNGG 3 cut(s) 602, 603, 658
MwoI GCNNNNNNNGC 3 cut(s) 221, 674, 701
NciI CCSGG 3 cut(s) 602, 603, 658
NdeII GATC 5 cut(s) 31, 49, 85, 472, 584
NlaIII CATG 6 cut(s) 22, 32, 143, 215, 343, 431
NlaIV GGNNCC 1 cut(s) 600
NmuCI GTSAC 1 cut(s) 244
NsbI TGCGCA 1 cut(s) 223
NspI RCATGY 2 cut(s) 215, 343
PaeI GCATGC 1 cut(s) 215
PagI TCATGA 2 cut(s) 28, 139
PaqCI CACCTGC 1 cut(s) 275
PceI AGGCCT 1 cut(s) 574
PciI ACATGT 1 cut(s) 339
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 6 cut(s) 326, 643, 694, 706, 709, 726
Ppu21I YACGTR 2 cut(s) 547, 581
PpuMI RGGWCCY 1 cut(s) 598
PscI ACATGT 1 cut(s) 339
PshAI GACNNNNGTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 598
PspN4I GGNNCC 1 cut(s) 600
PspPI GGNCC 1 cut(s) 598
PspPPI RGGWCCY 1 cut(s) 598
PstI CTGCAG 1 cut(s) 646
RsaI GTAC 1 cut(s) 579
RsaNI GTAC 1 cut(s) 578
RseI CAYNNNNRTG 1 cut(s) 17
SatI GCNGC 6 cut(s) 325, 642, 693, 705, 708, 725
Sau3AI GATC 5 cut(s) 31, 49, 85, 472, 584
Sau96I GGNCC 1 cut(s) 598
ScrFI CCNGG 3 cut(s) 602, 603, 658
SetI ASST 8 cut(s) 39, 205, 230, 269, 306, 549, 583, 740
SfcI CTRYAG 1 cut(s) 642
SinI GGWCC 1 cut(s) 598
SmaI CCCGGG 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 17
SnaBI TACGTA 1 cut(s) 581
SphI GCATGC 1 cut(s) 215
Sse9I AATT 1 cut(s) 293
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 4 cut(s) 189, 673, 692, 708
SspMI CTAG 3 cut(s) 476, 609, 627
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 3 cut(s) 600, 601, 656
StyI CCWWGG 1 cut(s) 626
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 3 cut(s) 306, 549, 583
TaqI TCGA 2 cut(s) 345, 523
TasI AATT 1 cut(s) 293
TauI GCSGC 2 cut(s) 695, 710
TfiI GAWTC 1 cut(s) 136
TscAI CASTG 2 cut(s) 281, 651
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 4 cut(s) 324, 641, 704, 724
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 474, 489
TspMI CCCGGG 1 cut(s) 601
TspRI CASTG 2 cut(s) 281, 651
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 1 cut(s) 293
XbaI TCTAGA 1 cut(s) 475
XceI RCATGY 2 cut(s) 215, 343
XmaI CCCGGG 1 cut(s) 601
XmaJI CCTAGG 1 cut(s) 626
XspI CTAG 3 cut(s) 476, 609, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.