pycom03g12230

Protein LATERAL ROOT PRIMORDIUM

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009808
Physical Location & Seq
Forward (+)
12644428 .. 12645840
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g12230.2

Sequence Viewer

Length: 1278 bp
ATGTCCATGTGGTCCTCCGCCCCTTCCAACAGACCCCTCACCTACGGCCACCTCCCTCCCGAGATGGCCGGCATGGTCGGCCTCCGCGACGTCTTTGTCGTTGCCCCAGCCGCCGCCTACCACCACAACACCCATCTCCACCACCACCCCGGCCACGACCCCAATCTCCTCTCCTCCTCCTCCGACCATCACCACTCCATCAATGCCTCCAACAATCCCGCAACCGCTCTTGGTGTCGGCGTCGGAGTCATTCCTCTTCTCACAGCAACCCCTTGTCTCGCCCCACCTAATATGTCAAATGTCGATGACGATGACGAAGACACTCACACACCTGCGGGTATGAGCATGGGAATGGGAATGAGTATGGGTATGATGAGTGGCGGCCATCAGCGCAACAACAGAAGCTCCTCAGCAGCAGGGGGAGGGGGAGGGATTCAATTGTGGCAGCAGAACCCGCCACATTTCTTCAAGAAAAATCATCATGAGGCCGCCGTTACTACTAATCTAGGCGGGATCCACTTAAACCAAGATGGAGGTGGTGGAGGAAGAGGCGGTGTTGGCGGCGGGGGAGGGAATAACATTATGAACGCTGGGTCTGCTGCTGCATCGTCGGGGTCGTCCACCATGACGTGTCAAGACTGCGGGAACCAGGCCAAGAAGGATTGTACGCACCGGCGGTGCAGGACGTGCTGCAAGAGCCGGGGTTTCGACTGTGCGACTCACGTGAAGAGCACGTGGGTGCCGGCGGCTCGGCGCAGGGAGCGGCAGCTGATGTCTGCAGCGGCTGGCGGTGGGGCTGGGTCTAATTCTGGGTCGACTTCTAGTGCCAAGAAGCCCAGACTGGTTGGCGCCTCTCAAACCACCCCTTCTCACACTTCCACTTCCAACACTACTCCCCCTAGAAGCTACGACACTAGTTCTAGTCACCAAGATGCTGGTTTCAAAGATCCATTGCCGGGACAAGTACGTGCCCCAGCGGTTTTCAAGTGTGTTCGAGTGACTGCCGTGGAGGACGGCGAGGATGAGTACGCATATCAGGCCGTTGTCAAAATTAACGGTCATGTGTTCAAAGGCTTCTTGTATGATCAAGGAGTTGATGGTAGAGAAGGGTTTCCTGACATCTCTGAATTGCATTTGGGGGGTGGTGGCACTACTAGTGGTGGCAATGGCGGAGGAAGAAATGGGGCTTCTTCTCCGATTCTTGACCCGTCGGATGTTTATGCCGCGTCTGGTGCAGGATTGCTGGGAGGTTCAAACTTTGGTAATCCAATAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

426

Amino Acids

43.58

Weight (kDa)

7.36

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009808)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12330 AT5G12330 AT5G12330 AT5G12330
fragaria_vesca FvH4_3g27920
malus_domestica MD03G1166900.v1.1 MD11G1186100.v1.1
prunus_persica Prupe.4G267200_v2.0.a1
pyrus_communis pycom03g12230 pycom12g08680
rosa_chinensis RchiOBHm_Chr5g0050641
rosa_laevigata RLG00000034715
rosa_multiflora Rmu_ssc0000350.1_g000034
rosa_roxburghii Rroxscaffold_1G00030150
rosa_rugosa Rorug05G0262400 Rorug05G0262500
rosa_samantha Rh5AG334700 Rh5BG344000 Rh5CG371800 Rh5DG357000
rosa_wichuraiana Rw5G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 2 cut(s) 739, 848
AccBSI CCGCTC 2 cut(s) 227, 763
AccI GTMKAC 1 cut(s) 815
AccII CGCG 2 cut(s) 87, 1226
AclWI GGATC 3 cut(s) 508, 521, 941
AcoI YGGCCR 4 cut(s) 46, 66, 151, 382
AcvI CACGTG 2 cut(s) 724, 735
AcyI GRCGYC 3 cut(s) 90, 240, 849
AfaI GTAC 3 cut(s) 667, 966, 1028
AfiI CCNNNNNNNGG 1 cut(s) 956
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 6 cut(s) 437, 469, 943, 985, 1069, 1254
AhlI ACTAGT 2 cut(s) 914, 1154
AjiI CACGTC 2 cut(s) 630, 687
AjnI CCWGG 1 cut(s) 648
AleI CACNNNNGTG 1 cut(s) 737
AluBI AGCT 3 cut(s) 405, 769, 906
AluI AGCT 3 cut(s) 405, 769, 906
Alw21I GWGCWC 1 cut(s) 734
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 3 cut(s) 508, 521, 941
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 8 cut(s) 46, 66, 79, 151, 382, 486, 651, 1038
ApeKI GCWGC 7 cut(s) 413, 445, 599, 602, 690, 766, 779
Asp700I GAANNNNTTC 1 cut(s) 1110
AspLEI GCGC 3 cut(s) 393, 756, 851
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 3 cut(s) 150, 701, 957
AsuHPI GGTGA 3 cut(s) 31, 182, 917
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 973
BamHI GGATCC 1 cut(s) 513
BanI GGYRCC 2 cut(s) 739, 848
BbrPI CACGTG 2 cut(s) 724, 735
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 734
BbvCI CCTCAGC 1 cut(s) 409
BbvI GCAGC 7 cut(s) 425, 457, 586, 589, 677, 778, 791
BccI CCATC 7 cut(s) 58, 141, 195, 206, 393, 524, 1091
BceAI ACGGC 5 cut(s) 61, 476, 989, 1025, 1030
BciT130I CCWGG 1 cut(s) 650
BclI TGATCA 1 cut(s) 1084
BcnI CCSGG 3 cut(s) 150, 701, 957
BcoDI GTCTC 1 cut(s) 281
BcuI ACTAGT 2 cut(s) 914, 1154
BfaI CTAG 6 cut(s) 506, 822, 900, 915, 921, 1155
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 852
BfuAI ACCTGC 1 cut(s) 340
Bme1390I CCNGG 4 cut(s) 150, 650, 701, 957
Bme18I GGWCC 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 59
BmgBI CACGTC 2 cut(s) 630, 687
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 4 cut(s) 515, 647, 741, 850
BmrFI CCNGG 4 cut(s) 150, 650, 701, 957
BmsI GCATC 2 cut(s) 614, 922
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 409
BpuMI CCSGG 3 cut(s) 150, 701, 957
BsaAI YACGTR 3 cut(s) 724, 735, 968
BsaHI GRCGYC 3 cut(s) 90, 240, 849
BsaJI CCNNGG 3 cut(s) 148, 700, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 956
Bse118I RCCGGY 3 cut(s) 68, 672, 742
Bse1I ACTGG 1 cut(s) 846
Bse3DI GCAATG 2 cut(s) 950, 1171
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 3 cut(s) 148, 700, 1005
BseGI GGATG 2 cut(s) 1027, 1219
BseLI CCNNNNNNNGG 1 cut(s) 956
BseMI GCAATG 2 cut(s) 950, 1171
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 5 cut(s) 158, 163, 166, 169, 397
BseSI GKGCMC 1 cut(s) 973
BseXI GCAGC 7 cut(s) 425, 457, 586, 589, 677, 778, 791
BseYI CCCAGC 5 cut(s) 106, 590, 797, 973, 1243
BsgI GTGCAG 2 cut(s) 700, 1254
Bsh1236I CGCG 2 cut(s) 87, 1226
BshFI GGCC 8 cut(s) 48, 68, 81, 153, 384, 488, 653, 1040
BshNI GGYRCC 2 cut(s) 739, 848
BsiHKAI GWGCWC 1 cut(s) 734
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 6 cut(s) 69, 150, 673, 700, 743, 956
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 1 cut(s) 956
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 972
BsnI GGCC 8 cut(s) 48, 68, 81, 153, 384, 488, 653, 1040
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 734, 973
Bsp143I GATC 3 cut(s) 513, 946, 1084
BspANI GGCC 8 cut(s) 48, 68, 81, 153, 384, 488, 653, 1040
BspCNI CTCAG 1 cut(s) 422
BspFNI CGCG 2 cut(s) 87, 1226
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 4 cut(s) 515, 647, 741, 850
BspMAI CTGCAG 1 cut(s) 781
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 3 cut(s) 508, 521, 941
BspQI GCTCTTC 1 cut(s) 722
BspT107I GGYRCC 2 cut(s) 739, 848
BsrBI CCGCTC 2 cut(s) 227, 763
BsrDI GCAATG 2 cut(s) 950, 1171
BsrFI RCCGGY 3 cut(s) 68, 672, 742
BsrI ACTGG 1 cut(s) 846
BssAI RCCGGY 3 cut(s) 68, 672, 742
BssECI CCNNGG 3 cut(s) 148, 700, 1005
BssMI GATC 3 cut(s) 513, 946, 1084
BssNI GRCGYC 3 cut(s) 90, 240, 849
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 713, 1058
Bst6I CTCTTC 3 cut(s) 261, 541, 722
BstACI GRCGYC 3 cut(s) 90, 240, 849
BstAPI GCANNNNNTGC 1 cut(s) 687
BstBAI YACGTR 3 cut(s) 724, 735, 968
BstC8I GCNNGC 3 cut(s) 70, 744, 787
BstDEI CTNAG 1 cut(s) 409
BstDSI CCRYGG 1 cut(s) 1005
BstF5I GGATG 2 cut(s) 1027, 1219
BstFNI CGCG 2 cut(s) 87, 1226
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 3 cut(s) 393, 756, 851
BstKTI GATC 3 cut(s) 516, 949, 1087
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 3 cut(s) 513, 946, 1084
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 4 cut(s) 148, 648, 699, 955
BstSFI CTRYAG 1 cut(s) 777
BstSLI GKGCMC 1 cut(s) 973
BstUI CGCG 2 cut(s) 87, 1226
BstV1I GCAGC 7 cut(s) 425, 457, 586, 589, 677, 778, 791
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 2 cut(s) 513, 946
BstXI CCANNNNNNTGG 1 cut(s) 935
BstYI RGATCY 2 cut(s) 513, 946
BsuRI GGCC 8 cut(s) 48, 68, 81, 153, 384, 488, 653, 1040
BtgI CCRYGG 1 cut(s) 1005
BtrI CACGTC 2 cut(s) 630, 687
BtsCI GGATG 2 cut(s) 1027, 1219
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 3 cut(s) 70, 744, 787
CaiI CAGNNNCTG 1 cut(s) 785
CciI TCATGA 1 cut(s) 481
CfoI GCGC 3 cut(s) 393, 756, 851
Cfr10I RCCGGY 3 cut(s) 68, 672, 742
Cfr13I GGNCC 1 cut(s) 12
CseI GACGC 2 cut(s) 229, 1215
Csp6I GTAC 3 cut(s) 666, 965, 1027
CviAII CATG 6 cut(s) 7, 73, 346, 482, 625, 1061
CviQI GTAC 3 cut(s) 666, 965, 1027
DdeI CTNAG 1 cut(s) 409
DinI GGCGCC 1 cut(s) 850
DpnI GATC 3 cut(s) 515, 948, 1086
DpnII GATC 3 cut(s) 513, 946, 1084
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 4 cut(s) 46, 66, 151, 382
Eam1104I CTCTTC 3 cut(s) 261, 541, 722
EarI CTCTTC 3 cut(s) 261, 541, 722
EciI GGCGGA 2 cut(s) 7, 1185
Eco47I GGWCC 1 cut(s) 12
Eco72I CACGTG 2 cut(s) 724, 735
Eco88I CYCGRG 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 648
EgeI GGCGCC 1 cut(s) 850
EheI GGCGCC 1 cut(s) 850
FaeI CATG 6 cut(s) 10, 76, 349, 485, 628, 1064
FaqI GGGAC 1 cut(s) 972
FatI CATG 6 cut(s) 6, 72, 345, 481, 624, 1060
FauI CCCGC 6 cut(s) 226, 328, 462, 503, 557, 635
FbaI TGATCA 1 cut(s) 1084
FblI GTMKAC 1 cut(s) 815
FokI GGATG 2 cut(s) 1034, 1226
FspBI CTAG 6 cut(s) 506, 822, 900, 915, 921, 1155
GlaI GCGC 3 cut(s) 392, 755, 850
GsaI CCCAGC 5 cut(s) 110, 594, 801, 977, 1247
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 8 cut(s) 48, 68, 81, 153, 384, 488, 653, 1040
HapII CCGG 6 cut(s) 69, 150, 673, 700, 743, 956
HgaI GACGC 2 cut(s) 229, 1215
HhaI GCGC 3 cut(s) 393, 756, 851
Hin1I GRCGYC 3 cut(s) 90, 240, 849
Hin1II CATG 6 cut(s) 10, 76, 349, 485, 628, 1064
Hin6I GCGC 3 cut(s) 391, 754, 849
HinP1I GCGC 3 cut(s) 391, 754, 849
HincII GTYRAC 1 cut(s) 816
HindII GTYRAC 1 cut(s) 816
HinfI GANTC 4 cut(s) 246, 433, 718, 1198
HpaII CCGG 6 cut(s) 69, 150, 673, 700, 743, 956
HphI GGTGA 3 cut(s) 31, 182, 917
Hpy166II GTNNAC 2 cut(s) 621, 816
Hpy188I TCNGA 5 cut(s) 184, 245, 1126, 1197, 1213
Hpy188III TCNNGA 6 cut(s) 59, 469, 482, 635, 1115, 1202
Hpy8I GTNNAC 2 cut(s) 621, 816
Hpy99I CGWCG 4 cut(s) 92, 245, 613, 1213
HpyAV CCTTC 4 cut(s) 33, 652, 876, 1100
HpyCH4III ACNGT 2 cut(s) 713, 1058
HpyCH4IV ACGT 6 cut(s) 90, 629, 686, 723, 734, 967
HpyCH4V TGCA 6 cut(s) 605, 681, 693, 779, 1132, 1235
HpyF3I CTNAG 1 cut(s) 409
HpySE526I ACGT 6 cut(s) 90, 629, 686, 723, 734, 967
Hsp92I GRCGYC 3 cut(s) 90, 240, 849
Hsp92II CATG 6 cut(s) 10, 76, 349, 485, 628, 1064
HspAI GCGC 3 cut(s) 391, 754, 849
KasI GGCGCC 1 cut(s) 848
KroI GCCGGC 2 cut(s) 68, 742
KroNI GCCGGC 2 cut(s) 70, 744
Ksp22I TGATCA 1 cut(s) 1084
Kzo9I GATC 3 cut(s) 513, 946, 1084
LguI GCTCTTC 1 cut(s) 722
LmnI GCTCC 2 cut(s) 410, 760
Lsp1109I GCAGC 7 cut(s) 425, 457, 586, 589, 677, 778, 791
LweI GCATC 2 cut(s) 614, 922
MaeI CTAG 6 cut(s) 506, 822, 900, 915, 921, 1155
MaeII ACGT 6 cut(s) 90, 629, 686, 723, 734, 967
MaeIII GTNAC 3 cut(s) 493, 923, 997
MalI GATC 3 cut(s) 515, 948, 1086
MbiI CCGCTC 2 cut(s) 227, 763
MboI GATC 3 cut(s) 513, 946, 1084
MboII GAAGA 7 cut(s) 248, 329, 457, 558, 739, 1182, 1188
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 2 cut(s) 513, 946
MhlI GDGCHC 2 cut(s) 734, 973
MluCI AATT 5 cut(s) 437, 805, 1050, 1127, 1273
Mly113I GGCGCC 1 cut(s) 849
MlyI GAGTC 2 cut(s) 255, 712
MmeI TCCRAC 6 cut(s) 51, 207, 223, 234, 909, 1191
MroNI GCCGGC 2 cut(s) 68, 742
MroXI GAANNNNTTC 1 cut(s) 1110
MseI TTAA 2 cut(s) 521, 1053
MslI CAYNNNNRTG 3 cut(s) 350, 737, 930
MspA1I CMGCKG 3 cut(s) 769, 782, 977
MspI CCGG 6 cut(s) 69, 150, 673, 700, 743, 956
MspR9I CCNGG 4 cut(s) 150, 650, 701, 957
MunI CAATTG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 2 cut(s) 87, 1226
NaeI GCCGGC 2 cut(s) 70, 744
NarI GGCGCC 1 cut(s) 849
NciI CCSGG 3 cut(s) 150, 701, 957
NdeII GATC 3 cut(s) 513, 946, 1084
NgoMIV GCCGGC 2 cut(s) 68, 742
NlaIII CATG 6 cut(s) 10, 76, 349, 485, 628, 1064
NlaIV GGNNCC 4 cut(s) 515, 647, 741, 850
NmeAIII GCCGAG 1 cut(s) 730
NmuCI GTSAC 2 cut(s) 923, 997
OliI CACNNNNGTG 1 cut(s) 737
PagI TCATGA 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 722
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 96, 614
PdiI GCCGGC 2 cut(s) 70, 744
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 2 cut(s) 433, 1198
PleI GAGTC 2 cut(s) 254, 712
PluTI GGCGCC 1 cut(s) 852
PmaCI CACGTG 2 cut(s) 724, 735
PmlI CACGTG 2 cut(s) 724, 735
PpsI GAGTC 2 cut(s) 254, 712
Ppu21I YACGTR 3 cut(s) 724, 735, 968
Psp6I CCWGG 1 cut(s) 648
PspCI CACGTG 2 cut(s) 724, 735
PspFI CCCAGC 5 cut(s) 106, 590, 797, 973, 1243
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 4 cut(s) 515, 647, 741, 850
PspPI GGNCC 1 cut(s) 12
PstI CTGCAG 1 cut(s) 781
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 2 cut(s) 513, 946
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 3 cut(s) 667, 966, 1028
RsaNI GTAC 3 cut(s) 666, 965, 1027
RseI CAYNNNNRTG 3 cut(s) 350, 737, 930
SalI GTCGAC 1 cut(s) 814
SapI GCTCTTC 1 cut(s) 722
SaqAI TTAA 2 cut(s) 521, 1053
Sau3AI GATC 3 cut(s) 513, 946, 1084
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 2 cut(s) 255, 712
ScrFI CCNGG 4 cut(s) 150, 650, 701, 957
SduI GDGCHC 2 cut(s) 734, 973
SfaNI GCATC 2 cut(s) 614, 922
SfcI CTRYAG 1 cut(s) 777
SfoI GGCGCC 1 cut(s) 850
SgrAI CRCCGGYG 1 cut(s) 672
SinI GGWCC 1 cut(s) 12
SmiMI CAYNNNNRTG 3 cut(s) 350, 737, 930
SpeI ACTAGT 2 cut(s) 914, 1154
Sse9I AATT 5 cut(s) 437, 805, 1050, 1127, 1273
SspDI GGCGCC 1 cut(s) 848
SspMI CTAG 6 cut(s) 506, 822, 900, 915, 921, 1155
StyD4I CCNGG 4 cut(s) 148, 648, 699, 955
TaaI ACNGT 2 cut(s) 713, 1058
TaiI ACGT 6 cut(s) 93, 632, 689, 726, 737, 970
TaqI TCGA 4 cut(s) 303, 708, 815, 994
TasI AATT 5 cut(s) 437, 805, 1050, 1127, 1273
TauI GCSGC 9 cut(s) 113, 116, 384, 491, 564, 749, 766, 785, 1226
TfiI GAWTC 2 cut(s) 433, 1198
Tru1I TTAA 2 cut(s) 521, 1053
Tru9I TTAA 2 cut(s) 521, 1053
TseFI GTSAC 2 cut(s) 923, 997
TseI GCWGC 7 cut(s) 413, 445, 599, 602, 690, 766, 779
Tsp45I GTSAC 2 cut(s) 923, 997
TspDTI ATGAA 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 12
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 815
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 6 cut(s) 506, 822, 900, 915, 921, 1155
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.