pycom03g20270

PDDEXK-like family of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007688
Physical Location & Seq
Reverse (-)
20788103 .. 20788697
595 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGCCTTTTTCAATGAAAATCCAACCGATTGATTTTCGCACGAGTCCTGAAGACCTGACTCGGTTCGAGCCGGTCAAGCCGGTAGTGGCGAAGTCGCGGCTCAAGCGGCTCTTCGAGCGGCCGTTTACGAACGTACTGAAAAACTCTGCACCGGCAGAGAAGGCCGGCCTCGGAAGCGAGGAGCCGCATTTCAACAAGGACGGCCTCGTCGGAGGCGGCAGCGGAGGATCGGCCGAGTTCGAGCCGAGTTCGATGTGCTTGACGAAGATGGTCCAGAATTTCATCGAGGACAGCACCGAGAAGCAATCATTGGCTGTGGCGCGGTGCAGCCGGAACCGCTGCAACTGCTTCAACGGCAACGACAGTTCGGAAGACGAATCGGAGCTGTTTGGCGGTTTTGGCGACTCCAACGTGGCATCCTCTGCCGAAGCATGCGAGCTTCTCAAGGGTTTGGTTTCGTGCCAGAGCGTGAGCGAGAGGAACTTGCTAGCCGACGCGGCGAAGATCGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDDEXK_6

Protein Analysis

172

Amino Acids

18.55

Weight (kDa)

5.18

Isoelectric Point (pI)

65.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDDEXK_6 PF04720 89 - 169 8.6e-07 PDDEXK-like family of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 118
AccII CGCG 3 cut(s) 97, 322, 497
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 2 cut(s) 119, 231
AcsI RAATTY 1 cut(s) 277
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 1 cut(s) 135
AgsI TTSAA 3 cut(s) 12, 193, 352
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 2 cut(s) 385, 439
AluI AGCT 2 cut(s) 385, 439
AlwI GGATC 1 cut(s) 235
AoxI GGCC 5 cut(s) 119, 162, 166, 202, 231
ApeKI GCWGC 3 cut(s) 219, 327, 339
ApoI RAATTY 1 cut(s) 277
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 271
AsuNHI GCTAGC 1 cut(s) 487
AvaII GGWCC 1 cut(s) 271
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 2 cut(s) 57, 378
BbvI GCAGC 3 cut(s) 231, 326, 339
BccI CCATC 1 cut(s) 262
BceAI ACGGC 3 cut(s) 106, 217, 370
BfaI CTAG 1 cut(s) 488
BglI GCCNNNNNGGC 1 cut(s) 497
BisI GCNGC 9 cut(s) 98, 107, 119, 185, 217, 220, 328, 340, 498
BlsI GCNGC 9 cut(s) 99, 108, 120, 186, 218, 221, 329, 341, 499
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 2 cut(s) 183, 335
BmsI GCATC 1 cut(s) 425
BmtI GCTAGC 1 cut(s) 491
BpiI GAAGAC 2 cut(s) 57, 378
BpuEI CTTGAG 2 cut(s) 86, 428
Bsa29I ATCGAT 2 cut(s) 507, 511
BsaJI CCNNGG 1 cut(s) 169
Bse118I RCCGGY 4 cut(s) 70, 79, 151, 164
BseCI ATCGAT 2 cut(s) 507, 511
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 416
BseRI GAGGAG 1 cut(s) 194
BseX3I CGGCCG 2 cut(s) 119, 231
BseXI GCAGC 3 cut(s) 231, 326, 339
BsgI GTGCAG 2 cut(s) 132, 346
Bsh1236I CGCG 3 cut(s) 97, 322, 497
Bsh1285I CGRYCG 3 cut(s) 122, 234, 511
BshFI GGCC 5 cut(s) 121, 164, 168, 204, 233
BshVI ATCGAT 2 cut(s) 507, 511
BsiEI CGRYCG 3 cut(s) 122, 234, 511
BsiSI CCGG 5 cut(s) 71, 80, 152, 165, 331
BsnI GGCC 5 cut(s) 121, 164, 168, 204, 233
Bsp143I GATC 3 cut(s) 227, 504, 508
BspANI GGCC 5 cut(s) 121, 164, 168, 204, 233
BspDI ATCGAT 2 cut(s) 507, 511
BspFNI CGCG 3 cut(s) 97, 322, 497
BspLI GGNNCC 2 cut(s) 183, 335
BspOI GCTAGC 1 cut(s) 491
BspPI GGATC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 118
BsrFI RCCGGY 4 cut(s) 70, 79, 151, 164
BssAI RCCGGY 4 cut(s) 70, 79, 151, 164
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 3 cut(s) 227, 504, 508
BssSI CACGAG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 40
Bst4CI ACNGT 1 cut(s) 365
Bst6I CTCTTC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 422
BstC8I GCNNGC 4 cut(s) 166, 433, 437, 489
BstF5I GGATG 1 cut(s) 416
BstFNI CGCG 3 cut(s) 97, 322, 497
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 3 cut(s) 230, 507, 511
BstMBI GATC 3 cut(s) 227, 504, 508
BstMCI CGRYCG 3 cut(s) 122, 234, 511
BstNSI RCATGY 1 cut(s) 435
BstUI CGCG 3 cut(s) 97, 322, 497
BstV1I GCAGC 3 cut(s) 231, 326, 339
BstV2I GAAGAC 2 cut(s) 57, 378
BstZI CGGCCG 2 cut(s) 119, 231
Bsu15I ATCGAT 2 cut(s) 507, 511
BsuRI GGCC 5 cut(s) 121, 164, 168, 204, 233
BsuTUI ATCGAT 2 cut(s) 507, 511
BtsCI GGATG 1 cut(s) 416
Cac8I GCNNGC 4 cut(s) 166, 433, 437, 489
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 4 cut(s) 70, 79, 151, 164
Cfr13I GGNCC 1 cut(s) 271
ClaI ATCGAT 2 cut(s) 507, 511
CseI GACGC 1 cut(s) 503
Csp6I GTAC 1 cut(s) 134
CviAII CATG 1 cut(s) 432
CviQI GTAC 1 cut(s) 134
DpnI GATC 3 cut(s) 229, 506, 510
DpnII GATC 3 cut(s) 227, 504, 508
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 2 cut(s) 119, 231
EagI CGGCCG 2 cut(s) 119, 231
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
EclXI CGGCCG 2 cut(s) 119, 231
Eco47I GGWCC 1 cut(s) 271
Eco52I CGGCCG 2 cut(s) 119, 231
Eco57I CTGAAG 1 cut(s) 69
FaeI CATG 1 cut(s) 435
FaiI YATR 1 cut(s) 433
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 1 cut(s) 431
Fnu4HI GCNGC 9 cut(s) 98, 107, 119, 185, 217, 220, 328, 340, 498
FokI GGATG 1 cut(s) 403
FseI GGCCGGCC 1 cut(s) 168
Fsp4HI GCNGC 9 cut(s) 98, 107, 119, 185, 217, 220, 328, 340, 498
FspBI CTAG 1 cut(s) 488
GlaI GCGC 1 cut(s) 321
GluI GCNGC 9 cut(s) 98, 107, 119, 185, 217, 220, 328, 340, 498
HaeIII GGCC 5 cut(s) 121, 164, 168, 204, 233
HapII CCGG 5 cut(s) 71, 80, 152, 165, 331
HgaI GACGC 1 cut(s) 503
HhaI GCGC 1 cut(s) 322
Hin1II CATG 1 cut(s) 435
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 4 cut(s) 43, 58, 377, 404
HpaII CCGG 5 cut(s) 71, 80, 152, 165, 331
Hpy166II GTNNAC 1 cut(s) 126
Hpy188I TCNGA 4 cut(s) 173, 212, 370, 382
Hpy188III TCNNGA 2 cut(s) 47, 274
Hpy8I GTNNAC 1 cut(s) 126
Hpy99I CGWCG 2 cut(s) 212, 497
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 2 cut(s) 132, 411
HpyCH4V TGCA 3 cut(s) 149, 327, 342
HpySE526I ACGT 2 cut(s) 132, 411
Hsp92II CATG 1 cut(s) 435
HspAI GCGC 1 cut(s) 320
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Kzo9I GATC 3 cut(s) 227, 504, 508
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 181, 382
LpnPI CCDG 9 cut(s) 60, 68, 84, 93, 165, 178, 287, 344, 476
Lsp1109I GCAGC 3 cut(s) 231, 326, 339
LweI GCATC 1 cut(s) 425
MaeI CTAG 1 cut(s) 488
MaeII ACGT 2 cut(s) 132, 411
MalI GATC 3 cut(s) 229, 506, 510
MbiI CCGCTC 1 cut(s) 118
MboI GATC 3 cut(s) 227, 504, 508
MboII GAAGA 5 cut(s) 62, 103, 277, 383, 514
MluCI AATT 1 cut(s) 277
MlyI GAGTC 3 cut(s) 52, 52, 398
MmeI TCCRAC 3 cut(s) 46, 190, 432
MnlI CCTC 8 cut(s) 172, 179, 206, 215, 218, 280, 430, 471
MroNI GCCGGC 1 cut(s) 164
MspA1I CMGCKG 2 cut(s) 222, 339
MspI CCGG 5 cut(s) 71, 80, 152, 165, 331
MvnI CGCG 3 cut(s) 97, 322, 497
NaeI GCCGGC 1 cut(s) 166
NdeII GATC 3 cut(s) 227, 504, 508
NgoMIV GCCGGC 1 cut(s) 164
NheI GCTAGC 1 cut(s) 487
NlaIII CATG 1 cut(s) 435
NlaIV GGNNCC 2 cut(s) 183, 335
NmeAIII GCCGAG 2 cut(s) 259, 270
NspI RCATGY 1 cut(s) 435
PaeI GCATGC 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 116
PdiI GCCGGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 377
PkrI GCNGC 9 cut(s) 99, 108, 120, 186, 218, 221, 329, 341, 499
Ple19I CGATCG 1 cut(s) 511
PleI GAGTC 3 cut(s) 51, 52, 398
PpsI GAGTC 3 cut(s) 51, 52, 398
PspN4I GGNNCC 2 cut(s) 183, 335
PspPI GGNCC 1 cut(s) 271
PvuI CGATCG 1 cut(s) 511
RigI GGCCGGCC 1 cut(s) 168
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 116
SatI GCNGC 9 cut(s) 98, 107, 119, 185, 217, 220, 328, 340, 498
Sau3AI GATC 3 cut(s) 227, 504, 508
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 3 cut(s) 52, 52, 398
SetI ASST 5 cut(s) 57, 135, 387, 414, 441
SfaNI GCATC 1 cut(s) 425
SinI GGWCC 1 cut(s) 271
SmlI CTYRAG 2 cut(s) 101, 443
SmoI CTYRAG 2 cut(s) 101, 443
SphI GCATGC 1 cut(s) 435
Sse9I AATT 1 cut(s) 277
SspMI CTAG 1 cut(s) 488
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 2 cut(s) 135, 414
TaqI TCGA 7 cut(s) 66, 114, 240, 251, 285, 507, 511
TasI AATT 1 cut(s) 277
TauI GCSGC 6 cut(s) 100, 109, 121, 187, 219, 500
TfiI GAWTC 1 cut(s) 377
TseI GCWGC 3 cut(s) 219, 327, 339
TspDTI ATGAA 2 cut(s) 29, 271
VpaK11BI GGWCC 1 cut(s) 271
XapI RAATTY 1 cut(s) 277
XceI RCATGY 1 cut(s) 435
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.