pycom03g21840

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006473
Physical Location & Seq
Forward (+)
22051727 .. 22055141
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1785 bp
ATGGGTTCGAAGCCATGGCTGTACCCGGCTCCGACCTATCGTACTCTCGAGACCTATTGGGATTGCGACGACGACGGCCCTGGTCCCCGCTGCGCTCACACCCTCACCGCCGTTGCCGCCACTAAGACCCATGGCCCCCGTCTCATCCTCTTCGGCGGTGCCACCGCCATTGAAGGCGGCGCCGCCTCCTCCGCCCCTGGCATCAGGTTAGCTGGGGTGACCAATTCTGTGCATTCGTATGATGTTCTCACCAGCAAATGGACTAGAATGAGGCCTGCAGGTGAGCCACCGTCTCCGAGGGCAGCTCACGCGGCGGCTGCGGTGGGCACTATGGTTGTATTTCAGGGTGGTATAGGTCCTGCTGGGCATTCCACAGATGATCTATACGTGCTGGACTTGACCAACGATAAGTTTAAATGGCACAGAGTGGTTGTACAAGGACAAGGTCCTGGGCCTCGCTACGGCCATGTAATGGACTTAGTTGCTCAACGATATCTTGTTACTGTCAGTGGGAACGATGGAAAAAGAGTACTTTCTGATGCCTGGGTTCTGGATACTGCTCAGAAACCTTATGTATGGGCTAGGCTGAGCCCAGAAGGTGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGTATGTTTTTGCTTTGTGGTGGTAGAGATATTTCTGGAGCGCCACTAGCAGACGCTTATGGACTGCTTATGCATAGAAATGGTCAGTGGGAGTGGACCCTTGCACCTGGAGTATCACCTTCACCAAGGTATCAACATGCGGCGGTTTTTGTTGGGGCAAGATTACATGTTACAGGAGGTACTCTTAGGGGAGGTCGTTGTATAGAAGGCGAAGCAGCTGTTGCAGTATTGGACACTGCTGCTGGAATTTGGTTAGACAGAAATGGGCTTGTGACCTCCTCACGCACAGGAAAGGGACAGAATGACTATGATCCTCCTTTGGAGCTCATGCGTCGTTGCCGCCACGCAGCTGCATCTCTTGGTGTTCGTATATACATTTATGGTGGTCTTAAGGGAGACATCCTGTTAGATGACTTCCTGGTTGCAGAAAATTCATTATTTCAGTCTGAAGGTAATTCGCCTGTTTTAACTTCTGAGAGATCCCCAACTGTATCAAGTCCCAGAATGTTTCATGCCAACACAAGTACTTTTGGAACATCATCTGATGGTGAACCTGAGAGTCCCTCGTCAAGTCGCTCGAGCATGGACAAAAGTTCAGTGGAGAAACTTCGGGAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAATGCTGTTTGGCAAGCTGTGCAGTCAACATCTGCTACTCCTGCTGAAGAAACATCTGTTTCAGATGATAACTCGCAAGTTGCAGAAACATTGTTGGATGGTAGTGACACTGAGGCTGATGTTCGCCTTCATCACAGAGCTGTTGTCGTTGCCAAAGAGGCTGTAGGTAACTTGGGTGGGATGGTAAGACAGTTGTCTTTGGACCAATTTGAAAATGAAAGCAGACGAATGATTCCAATGAATAATGACTTATCACATCCCAACAAAAAGTTCACCAAGCAGAAGTCTCCACAAGGGCTACATAAAAAGGTTATATCTACATTGTTGAGGCCTCGAAACTGGAAAGCCCCTGTCAATAGGAAGTTTTTCATGGATTCTTATGAAGTGGGAGAACTTTGTTATGCTGCTGAGCAGATCTTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCCCCTGTTAAAGTATTTGATCGATCTTCATGGACAGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

595

Amino Acids

64.3

Weight (kDa)

6.98

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 223 5e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 23 - 188 1.3e-24 FBX42, beta-propeller domain
Kelch_HCF PF13854 72 - 348 3.2e-15 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 74 - 352 1.9e-18 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 192 - 356 9e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 192 - 356 9.4e-10 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 203 - 281 1.8e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 269
AccB1I GGYRCC 2 cut(s) 158, 179
AccB7I CCANNNNNTGG 2 cut(s) 258, 472
AccBSI CCGCTC 1 cut(s) 642
AccII CGCG 1 cut(s) 311
AclWI GGATC 2 cut(s) 953, 1122
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 2 cut(s) 894, 1078
AcuI CTGAAG 3 cut(s) 1116, 1356, 1715
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 427
AfaI GTAC 6 cut(s) 23, 43, 435, 531, 829, 1174
AfiI CCNNNNNNNGG 3 cut(s) 258, 461, 472
AflII CTTAAG 1 cut(s) 1037
AflIII ACRYGT 1 cut(s) 814
AgsI TTSAA 2 cut(s) 173, 1502
AjnI CCWGG 6 cut(s) 79, 196, 448, 542, 754, 1065
AjuI GAANNNNNNNTTGG 2 cut(s) 1544, 1576
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 5 cut(s) 44, 146, 297, 1038, 1581
AlwI GGATC 2 cut(s) 953, 1122
Ama87I CYCGRG 2 cut(s) 47, 1225
AoxI GGCC 7 cut(s) 76, 133, 272, 452, 463, 605, 1619
ApoI RAATTY 2 cut(s) 894, 1078
Asp700I GAANNNNTTC 1 cut(s) 1655
AspLEI GCGC 3 cut(s) 95, 182, 691
AspS9I GGNCC 8 cut(s) 77, 83, 134, 356, 446, 452, 744, 1492
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 9 cut(s) 97, 229, 241, 293, 611, 756, 762, 1208, 1555
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 47, 1225
AvaII GGWCC 5 cut(s) 83, 356, 446, 744, 1492
BaeGI GKGCMC 2 cut(s) 329, 640
BaeI ACNNNNGTAYC 2 cut(s) 761, 794
BanI GGYRCC 2 cut(s) 158, 179
BanII GRGCYC 2 cut(s) 593, 975
Bbv12I GWGCWC 1 cut(s) 975
BbvCI CCTCAGC 1 cut(s) 1281
BccI CCATC 5 cut(s) 512, 641, 1187, 1382, 1465
BceAI ACGGC 3 cut(s) 91, 95, 478
BciT130I CCWGG 6 cut(s) 81, 198, 450, 544, 756, 1067
BciVI GTATCC 1 cut(s) 547
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 5 cut(s) 44, 146, 297, 1038, 1581
BfaI CTAG 4 cut(s) 264, 582, 615, 695
BfmI CTRYAG 2 cut(s) 276, 1452
BfoI RGCGCY 2 cut(s) 183, 692
BfrI CTTAAG 1 cut(s) 1037
BfuAI ACCTGC 1 cut(s) 269
BfuI GTATCC 1 cut(s) 547
BglI GCCNNNNNGGC 1 cut(s) 1448
BglII AGATCT 1 cut(s) 1704
BlpI GCTNAGC 2 cut(s) 587, 1698
BmcAI AGTACT 2 cut(s) 531, 1174
Bme1390I CCNGG 7 cut(s) 26, 81, 198, 450, 544, 756, 1067
Bme18I GGWCC 5 cut(s) 83, 356, 446, 744, 1492
BmeT110I CYCGRG 2 cut(s) 47, 1225
BmgT120I GGNCC 8 cut(s) 77, 83, 134, 356, 446, 452, 744, 1492
BmiI GGNNCC 6 cut(s) 30, 85, 136, 160, 181, 746
BmrFI CCNGG 7 cut(s) 26, 81, 198, 450, 544, 756, 1067
BmsI GCATC 3 cut(s) 210, 529, 1010
BoxI GACNNNNGTC 1 cut(s) 1483
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 1196, 1228
BpmI CTGGAG 2 cut(s) 705, 777
Bpu10I CCTNAGC 1 cut(s) 1281
Bpu1102I GCTNAGC 2 cut(s) 587, 1698
Bpu14I TTCGAA 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 1762
BsaAI YACGTR 1 cut(s) 388
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 8 cut(s) 14, 79, 130, 196, 296, 449, 543, 773
Bsc4I CCNNNNNNNGG 3 cut(s) 258, 461, 472
Bse1I ACTGG 2 cut(s) 633, 1634
BseBI CCWGG 6 cut(s) 81, 198, 450, 544, 756, 1067
BseCI ATCGAT 1 cut(s) 1762
BseDI CCNNGG 8 cut(s) 14, 79, 130, 196, 296, 449, 543, 773
BseGI GGATG 5 cut(s) 144, 1047, 1393, 1476, 1546
BseLI CCNNNNNNNGG 3 cut(s) 258, 461, 472
BseMII CTCAG 8 cut(s) 575, 578, 657, 1113, 1194, 1272, 1392, 1689
BseNI ACTGG 2 cut(s) 633, 1634
BseRI GAGGAG 2 cut(s) 178, 916
BseSI GKGCMC 2 cut(s) 329, 640
BseYI CCCAGC 2 cut(s) 212, 362
BsgI GTGCAG 1 cut(s) 1331
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 7 cut(s) 78, 135, 274, 454, 465, 607, 1621
BshNI GGYRCC 2 cut(s) 158, 179
BshVI ATCGAT 1 cut(s) 1762
BsiHKAI GWGCWC 1 cut(s) 975
BsiHKCI CYCGRG 2 cut(s) 47, 1225
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 4 cut(s) 69, 957, 1131, 1194
BslI CCNNNNNNNGG 3 cut(s) 258, 461, 472
BsmAI GTCTC 5 cut(s) 44, 146, 297, 1038, 1581
BsmBI CGTCTC 2 cut(s) 146, 297
BsmFI GGGAC 4 cut(s) 69, 957, 1131, 1194
BsmI GAATGC 2 cut(s) 232, 367
BsnI GGCC 7 cut(s) 78, 135, 274, 454, 465, 607, 1621
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 2 cut(s) 47, 1225
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 4 cut(s) 329, 593, 640, 975
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
Bsp1720I GCTNAGC 2 cut(s) 587, 1698
Bsp19I CCATGG 2 cut(s) 14, 130
BspANI GGCC 7 cut(s) 78, 135, 274, 454, 465, 607, 1621
BspCNI CTCAG 8 cut(s) 574, 579, 656, 1114, 1195, 1273, 1393, 1690
BspDI ATCGAT 1 cut(s) 1762
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 6 cut(s) 30, 85, 136, 160, 181, 746
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 2 cut(s) 953, 1122
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 158, 179
BspTI CTTAAG 1 cut(s) 1037
BspTNI GGTCTC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 642
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 2 cut(s) 633, 1634
BssECI CCNNGG 8 cut(s) 14, 79, 130, 196, 296, 449, 543, 773
BssMI GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 3 cut(s) 14, 130, 773
Bst2UI CCWGG 6 cut(s) 81, 198, 450, 544, 756, 1067
Bst4CI ACNGT 6 cut(s) 291, 505, 1138, 1482, 1726, 1777
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 1037
BstAPI GCANNNNNTGC 3 cut(s) 869, 1292, 1309
BstAUI TGTACA 1 cut(s) 433
BstBAI YACGTR 1 cut(s) 388
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 276, 640, 1305
BstDSI CCRYGG 2 cut(s) 14, 130
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 5 cut(s) 144, 1047, 1393, 1476, 1546
BstFNI CGCG 1 cut(s) 311
BstH2I RGCGCY 2 cut(s) 183, 692
BstHHI GCGC 3 cut(s) 95, 182, 691
BstKTI GATC 6 cut(s) 382, 961, 1130, 1707, 1762, 1766
BstMAI GTCTC 5 cut(s) 44, 146, 297, 1038, 1581
BstMBI GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
BstNI CCWGG 6 cut(s) 81, 198, 450, 544, 756, 1067
BstNSI RCATGY 2 cut(s) 788, 818
BstPAI GACNNNNGTC 1 cut(s) 1483
BstPI GGTNACC 1 cut(s) 217
BstSCI CCNGG 7 cut(s) 24, 79, 196, 448, 542, 754, 1065
BstSFI CTRYAG 2 cut(s) 276, 1452
BstSLI GKGCMC 2 cut(s) 329, 640
BstUI CGCG 1 cut(s) 311
BstX2I RGATCY 2 cut(s) 1127, 1704
BstYI RGATCY 2 cut(s) 1127, 1704
Bsu15I ATCGAT 1 cut(s) 1762
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 7 cut(s) 78, 135, 274, 454, 465, 607, 1621
BsuTUI ATCGAT 1 cut(s) 1762
BtgI CCRYGG 2 cut(s) 14, 130
BtsCI GGATG 5 cut(s) 144, 1047, 1393, 1476, 1546
BtsI GCAGTG 1 cut(s) 882
BtsIMutI CAGTG 6 cut(s) 514, 640, 740, 882, 1251, 1398
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 276, 640, 1305
CfoI GCGC 3 cut(s) 95, 182, 691
Cfr13I GGNCC 8 cut(s) 77, 83, 134, 356, 446, 452, 744, 1492
ClaI ATCGAT 1 cut(s) 1762
CseI GACGC 2 cut(s) 710, 968
Csp6I GTAC 6 cut(s) 22, 42, 434, 530, 828, 1173
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviQI GTAC 6 cut(s) 22, 42, 434, 530, 828, 1173
DinI GGCGCC 1 cut(s) 181
DpnI GATC 6 cut(s) 381, 960, 1129, 1706, 1761, 1765
DpnII GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
DraI TTTAAA 1 cut(s) 415
DraIII CACNNNGTG 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 973
Eco130I CCWWGG 3 cut(s) 14, 130, 773
Eco147I AGGCCT 3 cut(s) 274, 607, 1621
Eco24I GRGCYC 2 cut(s) 593, 975
Eco31I GGTCTC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 494
Eco47I GGWCC 5 cut(s) 83, 356, 446, 744, 1492
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 3 cut(s) 1116, 1356, 1715
Eco88I CYCGRG 2 cut(s) 47, 1225
Eco91I GGTNACC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 973
EcoO109I RGGNCCY 2 cut(s) 356, 446
EcoO65I GGTNACC 1 cut(s) 217
EcoRII CCWGG 6 cut(s) 79, 196, 448, 542, 754, 1065
EcoRV GATATC 1 cut(s) 494
EcoT14I CCWWGG 3 cut(s) 14, 130, 773
EcoT22I ATGCAT 1 cut(s) 723
EcoT38I GRGCYC 2 cut(s) 593, 975
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 3 cut(s) 14, 130, 773
Esp3I CGTCTC 2 cut(s) 146, 297
FaqI GGGAC 4 cut(s) 69, 957, 1131, 1194
FauI CCCGC 2 cut(s) 95, 647
FokI GGATG 5 cut(s) 131, 1034, 1400, 1483, 1533
FriOI GRGCYC 2 cut(s) 593, 975
FspBI CTAG 4 cut(s) 264, 582, 615, 695
GlaI GCGC 3 cut(s) 94, 181, 690
GsaI CCCAGC 2 cut(s) 216, 366
GsuI CTGGAG 2 cut(s) 705, 777
HaeII RGCGCY 2 cut(s) 183, 692
HaeIII GGCC 7 cut(s) 78, 135, 274, 454, 465, 607, 1621
HapII CCGG 1 cut(s) 26
HgaI GACGC 2 cut(s) 710, 968
HhaI GCGC 3 cut(s) 95, 182, 691
Hin1I GRCGYC 1 cut(s) 180
Hin6I GCGC 3 cut(s) 93, 180, 689
HinP1I GCGC 3 cut(s) 93, 180, 689
HincII GTYRAC 1 cut(s) 1317
HindII GTYRAC 1 cut(s) 1317
HinfI GANTC 3 cut(s) 1207, 1522, 1664
HpaII CCGG 1 cut(s) 26
HphI GGTGA 9 cut(s) 97, 229, 241, 293, 611, 756, 762, 1208, 1555
Hpy166II GTNNAC 4 cut(s) 744, 1199, 1317, 1563
Hpy188I TCNGA 9 cut(s) 33, 297, 538, 564, 646, 1096, 1123, 1192, 1354
Hpy188III TCNNGA 5 cut(s) 47, 49, 551, 684, 1259
Hpy8I GTNNAC 4 cut(s) 744, 1199, 1317, 1563
Hpy99I CGWCG 4 cut(s) 71, 74, 77, 984
HpyAV CCTTC 7 cut(s) 167, 590, 618, 777, 848, 1091, 1427
HpyCH4III ACNGT 6 cut(s) 291, 505, 1138, 1482, 1726, 1777
HpyCH4IV ACGT 1 cut(s) 387
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 180
HspAI GCGC 3 cut(s) 93, 180, 689
KasI GGCGCC 1 cut(s) 179
Kzo9I GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
LmnI GCTCC 4 cut(s) 34, 686, 970, 1717
LweI GCATC 3 cut(s) 210, 529, 1010
MaeI CTAG 4 cut(s) 264, 582, 615, 695
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 6 cut(s) 217, 499, 817, 919, 1394, 1457
MalI GATC 6 cut(s) 381, 960, 1129, 1706, 1761, 1765
MbiI CCGCTC 1 cut(s) 642
MboI GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
MboII GAAGA 4 cut(s) 142, 1349, 1721, 1758
MflI RGATCY 2 cut(s) 1127, 1704
MhlI GDGCHC 4 cut(s) 329, 593, 640, 975
MluCI AATT 5 cut(s) 223, 894, 1078, 1102, 1496
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 1216
MmeI TCCRAC 2 cut(s) 56, 1365
Mph1103I ATGCAT 1 cut(s) 723
MroXI GAANNNNTTC 1 cut(s) 1655
MseI TTAA 4 cut(s) 414, 1038, 1115, 1749
MslI CAYNNNNRTG 2 cut(s) 237, 726
MspA1I CMGCKG 4 cut(s) 90, 866, 998, 1735
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 7 cut(s) 26, 81, 198, 450, 544, 756, 1067
Mva1269I GAATGC 2 cut(s) 232, 367
MvaI CCWGG 6 cut(s) 81, 198, 450, 544, 756, 1067
MvnI CGCG 1 cut(s) 311
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 1 cut(s) 26
NcoI CCATGG 2 cut(s) 14, 130
NdeII GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
NlaIV GGNNCC 6 cut(s) 30, 85, 136, 160, 181, 746
NmuCI GTSAC 3 cut(s) 217, 919, 1394
NsiI ATGCAT 1 cut(s) 723
NspI RCATGY 2 cut(s) 788, 818
NspV TTCGAA 1 cut(s) 8
PaeR7I CTCGAG 2 cut(s) 47, 1225
PaqCI CACCTGC 1 cut(s) 269
PceI AGGCCT 3 cut(s) 274, 607, 1621
PciI ACATGT 1 cut(s) 814
PctI GAATGC 2 cut(s) 232, 367
PdmI GAANNNNTTC 1 cut(s) 1655
PfeI GAWTC 2 cut(s) 1522, 1664
PflFI GACNNNGTC 1 cut(s) 444
PflMI CCANNNNNTGG 2 cut(s) 258, 472
PleI GAGTC 1 cut(s) 1215
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 1215
Ppu21I YACGTR 1 cut(s) 388
PpuMI RGGWCCY 2 cut(s) 356, 446
PscI ACATGT 1 cut(s) 814
PshAI GACNNNNGTC 1 cut(s) 1483
Psp124BI GAGCTC 1 cut(s) 975
Psp5II RGGWCCY 2 cut(s) 356, 446
Psp6I CCWGG 6 cut(s) 79, 196, 448, 542, 754, 1065
PspEI GGTNACC 1 cut(s) 217
PspFI CCCAGC 2 cut(s) 212, 362
PspGI CCWGG 6 cut(s) 79, 196, 448, 542, 754, 1065
PspN4I GGNNCC 6 cut(s) 30, 85, 136, 160, 181, 746
PspPI GGNCC 8 cut(s) 77, 83, 134, 356, 446, 452, 744, 1492
PspPPI RGGWCCY 2 cut(s) 356, 446
PspXI VCTCGAGB 1 cut(s) 1225
PstI CTGCAG 1 cut(s) 280
PsuI RGATCY 2 cut(s) 1127, 1704
PsyI GACNNNGTC 1 cut(s) 444
PvuII CAGCTG 3 cut(s) 866, 998, 1735
RsaI GTAC 6 cut(s) 23, 43, 435, 531, 829, 1174
RsaNI GTAC 6 cut(s) 22, 42, 434, 530, 828, 1173
RseI CAYNNNNRTG 2 cut(s) 237, 726
SacI GAGCTC 1 cut(s) 975
SaqAI TTAA 4 cut(s) 414, 1038, 1115, 1749
Sau3AI GATC 6 cut(s) 379, 958, 1127, 1704, 1759, 1763
Sau96I GGNCC 8 cut(s) 77, 83, 134, 356, 446, 452, 744, 1492
SbfI CCTGCAGG 1 cut(s) 280
ScaI AGTACT 2 cut(s) 531, 1174
SchI GAGTC 1 cut(s) 1216
ScrFI CCNGG 7 cut(s) 26, 81, 198, 450, 544, 756, 1067
SdaI CCTGCAGG 1 cut(s) 280
SduI GDGCHC 4 cut(s) 329, 593, 640, 975
SfaNI GCATC 3 cut(s) 210, 529, 1010
SfcI CTRYAG 2 cut(s) 276, 1452
SfoI GGCGCC 1 cut(s) 181
Sfr274I CTCGAG 2 cut(s) 47, 1225
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 5 cut(s) 83, 356, 446, 744, 1492
SlaI CTCGAG 2 cut(s) 47, 1225
SmiMI CAYNNNNRTG 2 cut(s) 237, 726
SmlI CTYRAG 3 cut(s) 47, 1037, 1225
SmoI CTYRAG 3 cut(s) 47, 1037, 1225
Sse8387I CCTGCAGG 1 cut(s) 280
Sse9I AATT 5 cut(s) 223, 894, 1078, 1102, 1496
SseBI AGGCCT 3 cut(s) 274, 607, 1621
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 4 cut(s) 264, 582, 615, 695
SstI GAGCTC 1 cut(s) 975
StuI AGGCCT 3 cut(s) 274, 607, 1621
StyD4I CCNGG 7 cut(s) 24, 79, 196, 448, 542, 754, 1065
StyI CCWWGG 3 cut(s) 14, 130, 773
TaaI ACNGT 6 cut(s) 291, 505, 1138, 1482, 1726, 1777
TaiI ACGT 1 cut(s) 390
TaqI TCGA 5 cut(s) 8, 48, 1226, 1624, 1762
TasI AATT 5 cut(s) 223, 894, 1078, 1102, 1496
TatI WGTACW 3 cut(s) 433, 529, 1172
TauI GCSGC 7 cut(s) 119, 180, 185, 314, 317, 791, 990
TfiI GAWTC 2 cut(s) 1522, 1664
Tru1I TTAA 4 cut(s) 414, 1038, 1115, 1749
Tru9I TTAA 4 cut(s) 414, 1038, 1115, 1749
TscAI CASTG 6 cut(s) 514, 640, 740, 889, 1251, 1405
TseFI GTSAC 3 cut(s) 217, 919, 1394
Tsp45I GTSAC 3 cut(s) 217, 919, 1394
TspDTI ATGAA 8 cut(s) 1071, 1148, 1409, 1521, 1544, 1648, 1686, 1758
TspRI CASTG 6 cut(s) 514, 640, 740, 889, 1251, 1405
Tth111I GACNNNGTC 1 cut(s) 444
Van91I CCANNNNNTGG 2 cut(s) 258, 472
Vha464I CTTAAG 1 cut(s) 1037
VpaK11BI GGWCC 5 cut(s) 83, 356, 446, 744, 1492
XapI RAATTY 2 cut(s) 894, 1078
XceI RCATGY 2 cut(s) 788, 818
XhoI CTCGAG 2 cut(s) 47, 1225
XmnI GAANNNNTTC 1 cut(s) 1655
XspI CTAG 4 cut(s) 264, 582, 615, 695
ZrmI AGTACT 2 cut(s) 531, 1174
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.