pycom03g22610
NAC Family

Dol-P-Man Man(5)GlcNAc(2)-PP-Dol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006111
Physical Location & Seq
Reverse (-)
22677765 .. 22681113
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1335 bp
ATGGCGGGCAGATCGACGGCGGCGACGCGGCCGAAACCCCCGCAGCGGCGGCGGATTTCCAAATCCTCGAACCCCAAATTTCTCAAAGACCCTAAATTACTCTTCTCTTCCGCCTTGCTCCTTTGCGATGCGATCCTAGTCGCCCTTATCATCGCCTATGTCCCCTATACGAAGATAGATTGGGACGCGTACATGTCACAAGTTAGTGGGTTTCTTGGTGGAGAGAGAGACTACGCTAACTTAAAAGGCGACACAGGGCCGCTGGTTTATCCTGCTGGCTTCCTCTACTTTTATTCTGCCATTCAGTATGTTACAGGAGGCCAAGTTCATCCAGCTCAGATTTTGTTCGGGATTTTGTACATTATAAACTTGGGGATCATATTGTTCATCTATGGGAAGACAGATGTGGTTCCATGGTGGGCTTTGATCTTGCTTTGTCTATCGAAAAGGGTTCATTCTATTTTTGTGCTTCGTCTTTTTAATGACTGTTTGGCCATGATGCTCCTGTATGCTTCGTTGGCCTCACTTCTTTACCAGAAGTGGCATCTAGGACTGATCATTTTCAGTGGAGCTGTTTCCATAAAGATGAATGTGCTTCTCTATGCTCCACCTCTACTACTCCTCATGTTAAAGGCTATGAGTATCGGTAGAGTGATATCAGCTTTAGCTGGTGCGGCACTTGTACAGATACTGTTGGGGATGCCTTTCATCATGTCTCATCCGTTTGCATACATCTCAAGAGCCTTTAATCTCGGTCGAGTTTTTATTCACTTCTGGTCTGTTAATTTCAAGTTTGTTCCTGAGCCAATATTTGTATCGAAGGCATTTGCAATCTCTTTGCTGATTGCTCACCTTGGTTTACTCACTGCTTTTGCTCATTATAGATGGTGCATGCATGAAGGTGGACTCTTCAAATTTTTGTATTCTAGACTCGATCCTATAAAACTCAAATTTGCTCTTACAAGTTCATTCCCTTTGAAGAAGTCCTTCAATATCCATTCATCCAACAGGGTCCTTAGGAAAGAATATATTGTGACGACTATGTTTGTTGGGAATTTCATCGGCATTGTATGTGCTCGATCATTGCATTATCAGTTCTACTCGTGGTATTTCCATAGCTTACCATATCTATTGTGGAAAACGCCTTTTCCCACAGTACTACGCATATTGTTGTTTATCGGCATGGAGTTTTGCTGGAACGTCTTTCCTTCAAACCTATACTCATCGGCGATGCTTTTTTTTCTCCACATAGTAATACTGGTCGGTCTATGGTCTGCCCCGTCTGAATATCCTTTTGTGGACAACAAAGCATCGCCTGAAAACAAAGATAAATGA

Protein Analysis

445

Amino Acids

50.42

Weight (kDa)

9.75

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALG3 PF05208 38 - 409 1.1e-135 ALG3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 365
AccII CGCG 2 cut(s) 28, 188
AclWI GGATC 3 cut(s) 127, 383, 929
AcoI YGGCCR 2 cut(s) 29, 492
AcsI RAATTY 4 cut(s) 77, 914, 950, 1054
AfaI GTAC 4 cut(s) 191, 359, 684, 1158
AfiI CCNNNNNNNGG 1 cut(s) 45
AflIII ACRYGT 2 cut(s) 186, 192
AgsI TTSAA 5 cut(s) 790, 913, 979, 991, 1212
AloI GAACNNNNNNTCC 2 cut(s) 309, 341
AluBI AGCT 5 cut(s) 335, 572, 662, 668, 1119
AluI AGCT 5 cut(s) 335, 572, 662, 668, 1119
Alw21I GWGCWC 1 cut(s) 1078
Alw26I GTCTC 2 cut(s) 222, 720
AlwI GGATC 3 cut(s) 127, 383, 929
AlwNI CAGNNNCTG 1 cut(s) 691
AoxI GGCC 5 cut(s) 29, 257, 319, 492, 519
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 4 cut(s) 77, 914, 950, 1054
Asp700I GAANNNNTTC 1 cut(s) 986
AspS9I GGNCC 2 cut(s) 257, 1012
AsuHPI GGTGA 1 cut(s) 842
AvaII GGWCC 1 cut(s) 1012
AxyI CCTNAGG 1 cut(s) 1016
BalI TGGCCA 1 cut(s) 494
BauI CACGAG 1 cut(s) 1102
BbsI GAAGAC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 1078
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 879
BceAI ACGGC 1 cut(s) 33
BclI TGATCA 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 222, 720
BfaI CTAG 3 cut(s) 137, 548, 927
BisI GCNGC 7 cut(s) 21, 29, 44, 47, 50, 260, 675
BlsI GCNGC 7 cut(s) 22, 30, 45, 48, 51, 261, 676
BmcAI AGTACT 1 cut(s) 1158
Bme18I GGWCC 1 cut(s) 1012
BmgT120I GGNCC 2 cut(s) 257, 1012
BmiI GGNNCC 2 cut(s) 411, 1013
BmsI GCATC 6 cut(s) 118, 489, 553, 690, 1221, 1319
BpiI GAAGAC 1 cut(s) 404
Bpu10I CCTNAGC 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 721
BsaJI CCNNGG 2 cut(s) 413, 853
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 1263
Bse21I CCTNAGG 1 cut(s) 1016
Bse3DI GCAATG 1 cut(s) 1082
BseDI CCNNGG 2 cut(s) 413, 853
BseGI GGATG 4 cut(s) 328, 705, 718, 1001
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 2 cut(s) 350, 792
BseNI ACTGG 1 cut(s) 1263
BseRI GAGGAG 1 cut(s) 611
BseX3I CGGCCG 1 cut(s) 29
BseXI GCAGC 1 cut(s) 55
Bsh1236I CGCG 2 cut(s) 28, 188
Bsh1285I CGRYCG 2 cut(s) 32, 757
BshFI GGCC 5 cut(s) 31, 259, 321, 494, 521
BsiEI CGRYCG 2 cut(s) 32, 757
BsiHKAI GWGCWC 1 cut(s) 1078
BslFI GGGAC 2 cut(s) 146, 197
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 2 cut(s) 222, 720
BsmFI GGGAC 2 cut(s) 146, 197
BsnI GGCC 5 cut(s) 31, 259, 321, 494, 521
Bsp1286I GDGCHC 1 cut(s) 1078
Bsp1407I TGTACA 2 cut(s) 357, 682
Bsp143I GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
Bsp19I CCATGG 1 cut(s) 413
BspANI GGCC 5 cut(s) 31, 259, 321, 494, 521
BspCNI CTCAG 2 cut(s) 349, 793
BspFNI CGCG 2 cut(s) 28, 188
BspLI GGNNCC 2 cut(s) 411, 1013
BspPI GGATC 3 cut(s) 127, 383, 929
BsrDI GCAATG 1 cut(s) 1082
BsrGI TGTACA 2 cut(s) 357, 682
BsrI ACTGG 1 cut(s) 1263
BssECI CCNNGG 2 cut(s) 413, 853
BssMI GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
BssSI CACGAG 1 cut(s) 1102
BssT1I CCWWGG 2 cut(s) 413, 853
Bst2BI CACGAG 1 cut(s) 1102
Bst4CI ACNGT 3 cut(s) 488, 693, 1156
Bst6I CTCTTC 3 cut(s) 107, 112, 914
BstAUI TGTACA 2 cut(s) 357, 682
BstC8I GCNNGC 3 cut(s) 7, 277, 893
BstDEI CTNAG 3 cut(s) 336, 801, 1016
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 4 cut(s) 328, 705, 718, 1001
BstFNI CGCG 2 cut(s) 28, 188
BstKTI GATC 7 cut(s) 14, 135, 378, 429, 558, 937, 1082
BstMAI GTCTC 2 cut(s) 222, 720
BstMBI GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
BstMCI CGRYCG 2 cut(s) 32, 757
BstMWI GCNNNNNNNGC 3 cut(s) 49, 518, 674
BstNSI RCATGY 2 cut(s) 196, 895
BstUI CGCG 2 cut(s) 28, 188
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 404
BstZI CGGCCG 1 cut(s) 29
Bsu36I CCTNAGG 1 cut(s) 1016
BsuRI GGCC 5 cut(s) 31, 259, 321, 494, 521
BtgI CCRYGG 1 cut(s) 413
BtgZI GCGATG 4 cut(s) 136, 141, 1244, 1296
BtsCI GGATG 4 cut(s) 328, 705, 718, 1001
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 2 cut(s) 571, 864
Cac8I GCNNGC 3 cut(s) 7, 277, 893
CaiI CAGNNNCTG 1 cut(s) 691
Cfr13I GGNCC 2 cut(s) 257, 1012
CseI GACGC 2 cut(s) 34, 194
Csp6I GTAC 4 cut(s) 190, 358, 683, 1157
CviAII CATG 8 cut(s) 193, 414, 496, 625, 712, 892, 896, 1183
CviQI GTAC 4 cut(s) 190, 358, 683, 1157
DdeI CTNAG 3 cut(s) 336, 801, 1016
DpnI GATC 7 cut(s) 13, 134, 377, 428, 557, 936, 1081
DpnII GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
EaeI YGGCCR 2 cut(s) 29, 492
EagI CGGCCG 1 cut(s) 29
Eam1104I CTCTTC 3 cut(s) 107, 112, 914
EarI CTCTTC 3 cut(s) 107, 112, 914
EciI GGCGGA 2 cut(s) 67, 100
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 2 cut(s) 413, 853
Eco32I GATATC 1 cut(s) 657
Eco47I GGWCC 1 cut(s) 1012
Eco52I CGGCCG 1 cut(s) 29
Eco81I CCTNAGG 1 cut(s) 1016
EcoO109I RGGNCCY 1 cut(s) 1012
EcoRV GATATC 1 cut(s) 657
EcoT14I CCWWGG 2 cut(s) 413, 853
EcoT22I ATGCAT 1 cut(s) 897
ErhI CCWWGG 2 cut(s) 413, 853
FaeI CATG 8 cut(s) 196, 417, 499, 628, 715, 895, 899, 1186
FalI AAGNNNNNCTT 2 cut(s) 971, 1003
FaqI GGGAC 2 cut(s) 146, 197
FatI CATG 8 cut(s) 192, 413, 495, 624, 711, 891, 895, 1182
FauI CCCGC 1 cut(s) 48
FbaI TGATCA 1 cut(s) 555
Fnu4HI GCNGC 7 cut(s) 21, 29, 44, 47, 50, 260, 675
FokI GGATG 4 cut(s) 315, 705, 712, 988
Fsp4HI GCNGC 7 cut(s) 21, 29, 44, 47, 50, 260, 675
FspBI CTAG 3 cut(s) 137, 548, 927
GluI GCNGC 7 cut(s) 21, 29, 44, 47, 50, 260, 675
HaeIII GGCC 5 cut(s) 31, 259, 321, 494, 521
HgaI GACGC 2 cut(s) 34, 194
Hin1II CATG 8 cut(s) 196, 417, 499, 628, 715, 895, 899, 1186
HinfI GANTC 2 cut(s) 906, 930
HphI GGTGA 1 cut(s) 842
Hpy166II GTNNAC 3 cut(s) 860, 905, 1300
Hpy188I TCNGA 2 cut(s) 339, 1285
Hpy188III TCNNGA 4 cut(s) 349, 738, 800, 927
Hpy8I GTNNAC 3 cut(s) 860, 905, 1300
Hpy99I CGWCG 2 cut(s) 19, 28
HpyAV CCTTC 4 cut(s) 814, 893, 997, 1218
HpyCH4III ACNGT 3 cut(s) 488, 693, 1156
HpyCH4IV ACGT 1 cut(s) 1200
HpyCH4V TGCA 5 cut(s) 728, 830, 891, 895, 1087
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 518, 674
HpyF3I CTNAG 3 cut(s) 336, 801, 1016
HpySE526I ACGT 1 cut(s) 1200
Hsp92II CATG 8 cut(s) 196, 417, 499, 628, 715, 895, 899, 1186
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
LmnI GCTCC 4 cut(s) 123, 507, 569, 610
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 6 cut(s) 118, 489, 553, 690, 1221, 1319
MaeI CTAG 3 cut(s) 137, 548, 927
MaeII ACGT 1 cut(s) 1200
MaeIII GTNAC 3 cut(s) 195, 310, 1033
MalI GATC 7 cut(s) 13, 134, 377, 428, 557, 936, 1081
MboI GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
MboII GAAGA 6 cut(s) 94, 99, 184, 409, 901, 991
MhlI GDGCHC 1 cut(s) 1078
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 6 cut(s) 77, 95, 784, 914, 950, 1054
MluI ACGCGT 1 cut(s) 186
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 2 cut(s) 900, 924
MmeI TCCRAC 1 cut(s) 1029
MnlI CCTC 6 cut(s) 76, 293, 311, 532, 621, 632
Mox20I TGGCCA 1 cut(s) 494
Mph1103I ATGCAT 1 cut(s) 897
MroXI GAANNNNTTC 1 cut(s) 986
MscI TGGCCA 1 cut(s) 494
MseI TTAA 5 cut(s) 242, 480, 629, 747, 783
MslI CAYNNNNRTG 2 cut(s) 584, 900
Msp20I TGGCCA 1 cut(s) 494
MspA1I CMGCKG 2 cut(s) 46, 262
MvnI CGCG 2 cut(s) 28, 188
MwoI GCNNNNNNNGC 3 cut(s) 49, 518, 674
NcoI CCATGG 1 cut(s) 413
NdeII GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
NlaIII CATG 8 cut(s) 196, 417, 499, 628, 715, 895, 899, 1186
NlaIV GGNNCC 2 cut(s) 411, 1013
NmuCI GTSAC 2 cut(s) 195, 1033
NsiI ATGCAT 1 cut(s) 897
NspI RCATGY 2 cut(s) 196, 895
PaeI GCATGC 1 cut(s) 895
PciI ACATGT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 986
PkrI GCNGC 7 cut(s) 22, 30, 45, 48, 51, 261, 676
PleI GAGTC 2 cut(s) 900, 924
PpsI GAGTC 2 cut(s) 900, 924
PpuMI RGGWCCY 1 cut(s) 1012
PscI ACATGT 1 cut(s) 192
PsiI TTATAA 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 1012
PspN4I GGNNCC 2 cut(s) 411, 1013
PspPI GGNCC 2 cut(s) 257, 1012
PspPPI RGGWCCY 1 cut(s) 1012
PstNI CAGNNNCTG 1 cut(s) 691
RsaI GTAC 4 cut(s) 191, 359, 684, 1158
RsaNI GTAC 4 cut(s) 190, 358, 683, 1157
RseI CAYNNNNRTG 2 cut(s) 584, 900
SaqAI TTAA 5 cut(s) 242, 480, 629, 747, 783
SatI GCNGC 7 cut(s) 21, 29, 44, 47, 50, 260, 675
Sau3AI GATC 7 cut(s) 11, 132, 375, 426, 555, 934, 1079
Sau96I GGNCC 2 cut(s) 257, 1012
ScaI AGTACT 1 cut(s) 1158
SchI GAGTC 2 cut(s) 900, 924
SduI GDGCHC 1 cut(s) 1078
SfaNI GCATC 6 cut(s) 118, 489, 553, 690, 1221, 1319
SinI GGWCC 1 cut(s) 1012
SmiMI CAYNNNNRTG 2 cut(s) 584, 900
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
SphI GCATGC 1 cut(s) 895
Sse9I AATT 6 cut(s) 77, 95, 784, 914, 950, 1054
SspI AATATT 1 cut(s) 810
SspMI CTAG 3 cut(s) 137, 548, 927
StyI CCWWGG 2 cut(s) 413, 853
TaaI ACNGT 3 cut(s) 488, 693, 1156
TaiI ACGT 1 cut(s) 1203
TaqI TCGA 7 cut(s) 14, 68, 443, 757, 818, 933, 1078
TaqII GACCGA 2 cut(s) 743, 1253
TasI AATT 6 cut(s) 77, 95, 784, 914, 950, 1054
TatI WGTACW 3 cut(s) 357, 682, 1156
TauI GCSGC 6 cut(s) 23, 31, 49, 52, 262, 677
Tru1I TTAA 5 cut(s) 242, 480, 629, 747, 783
Tru9I TTAA 5 cut(s) 242, 480, 629, 747, 783
TscAI CASTG 2 cut(s) 571, 871
TseFI GTSAC 2 cut(s) 195, 1033
TseI GCWGC 1 cut(s) 43
Tsp45I GTSAC 2 cut(s) 195, 1033
TspDTI ATGAA 9 cut(s) 317, 376, 443, 602, 697, 912, 957, 990, 1048
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 2 cut(s) 571, 871
VpaK11BI GGWCC 1 cut(s) 1012
XapI RAATTY 4 cut(s) 77, 914, 950, 1054
XbaI TCTAGA 1 cut(s) 926
XceI RCATGY 2 cut(s) 196, 895
XcmI CCANNNNNNNNNTGG 1 cut(s) 1131
XmnI GAANNNNTTC 1 cut(s) 986
XspI CTAG 3 cut(s) 137, 548, 927
ZrmI AGTACT 1 cut(s) 1158
Zsp2I ATGCAT 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.