pycom04g04580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014747
Physical Location & Seq
Reverse (-)
4197322 .. 4198308
987 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGTACGACAACGGCGATTCCTTCGAGTTTCCCGACGACTCGTCGCCTGACCTGAATTCTGGGGATGACGTCACCCCCACCGCAATCGTTATGGTCTCGCCTCCGGAGACATCGGACGACGAAGATCCCAACTGCAGCAGCAGCACCAACAACAACAGAGCCTCCCAAGACGTCTTCCAGACCCCGCCCGAAGACGCGCATCCACCGACCTCCGATGGTATTACTCCGTTTCCGGAAGTCGATCAGAGCGACGATGATGACGGTCGTCCGTTCGAGGATGCCGGAGATGGGCGCGGCGTAACCGTCCCCTCCGATGATGGTTTCACGGACGGCACTGCCGGTGGGGTTGATTTGGGGCGGGACACGGATCTAGAGTTTTCTAAGGCAGAAGCGATGGGAATTGCGGGTTTTGTGACGGAATTGGCTTGGGAACGCGGTCGATTTAAGTCGCCGTTGAAGAAATTGAGGGTATCCGAGCACGAGGTGGGGAAATTGGAATTTAAGGAAATGGTTCCGCATAGTCCCGGCTGTAACTCTGGCAAGAGTCTTGAAATTGACGGTGGCGATGAGAAGTCGAGCGAGGAAATATTGGGGGTGAGGAAGCTGGACTCTTCCGAAGACGAGCATAAGAGTTGTATGGAAGAATCTAAATCGGACAGTGAGGAAGAGGAAAGCATTCAAGACATACTGAAGAGGACGCCTAATGTGACCATTGAGGAATTGCATCGAATAGAGAGGCTGTGGCTGCAGCGGGTGCTGCCTCGATCCATCGGTGGGCAGGCAGGATCAAGTGCAAATGGCGCCAAGGAGGGGAAGCCGGAGGAGGTTGCTATATTGGATGTGTTGAAACTTCTGAAGGACAAATATGATGGCAATGGTAATGGTAATGGTAATGATGAGATTGACAAAAGTAGTTTTTTGGAGATAAGTAAGCGCCGAGGCATGACTTTCCCCCGACCGTCGTGGTGGCCGGAAGGCGGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

35.77

Weight (kDa)

4.35

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 72, 174
AccB1I GGYRCC 1 cut(s) 800
AccII CGCG 3 cut(s) 197, 294, 435
AccIII TCCGGA 2 cut(s) 103, 232
AciI CCGC 9 cut(s) 81, 185, 294, 358, 404, 435, 515, 751, 978
AclWI GGATC 4 cut(s) 119, 375, 759, 793
AcoI YGGCCR 1 cut(s) 968
AcsI RAATTY 2 cut(s) 55, 497
AcuI CTGAAG 2 cut(s) 710, 875
AcyI GRCGYC 4 cut(s) 69, 171, 698, 801
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 288, 774, 810, 977
AgsI TTSAA 4 cut(s) 457, 551, 680, 847
AhdI GACNNNNNGTC 1 cut(s) 40
AloI GAACNNNNNNTCC 1 cut(s) 965
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 2 cut(s) 100, 101
AlwI GGATC 4 cut(s) 119, 375, 759, 793
Aor13HI TCCGGA 2 cut(s) 103, 232
AoxI GGCC 1 cut(s) 968
ApeKI GCWGC 6 cut(s) 135, 138, 141, 745, 748, 757
ApoI RAATTY 2 cut(s) 55, 497
Asp700I GAANNNNTTC 2 cut(s) 510, 675
AspLEI GCGC 4 cut(s) 199, 294, 803, 936
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 64, 607
BanI GGYRCC 1 cut(s) 800
BauI CACGAG 1 cut(s) 479
BbsI GAAGAC 3 cut(s) 166, 198, 624
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 6 cut(s) 147, 150, 153, 732, 744, 760
BccI CCATC 6 cut(s) 209, 281, 311, 388, 776, 863
BceAI ACGGC 3 cut(s) 28, 346, 436
BciVI GTATCC 1 cut(s) 481
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 100, 101
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 2 cut(s) 133, 746
BfoI RGCGCY 2 cut(s) 804, 937
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 7 cut(s) 136, 139, 142, 295, 746, 749, 758
BlsI GCNGC 7 cut(s) 137, 140, 143, 296, 747, 750, 759
Bme1390I CCNGG 1 cut(s) 525
BmeRI GACNNNNNGTC 1 cut(s) 40
BmiI GGNNCC 2 cut(s) 513, 802
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 3 cut(s) 208, 268, 733
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 3 cut(s) 166, 198, 624
BpuMI CCSGG 1 cut(s) 525
BsaHI GRCGYC 4 cut(s) 69, 171, 698, 801
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 804, 937
BsaWI WCCGGW 2 cut(s) 103, 232
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 774, 810, 977
Bse118I RCCGGY 1 cut(s) 338
Bse3DI GCAATG 1 cut(s) 880
BseAI TCCGGA 2 cut(s) 103, 232
BseDI CCNNGG 2 cut(s) 804, 937
BseGI GGATG 4 cut(s) 70, 199, 283, 844
BseLI CCNNNNNNNGG 4 cut(s) 288, 774, 810, 977
BseMI GCAATG 1 cut(s) 880
BseRI GAGGAG 1 cut(s) 836
BseXI GCAGC 6 cut(s) 147, 150, 153, 732, 744, 760
Bsh1236I CGCG 3 cut(s) 197, 294, 435
Bsh1285I CGRYCG 3 cut(s) 265, 439, 959
BshFI GGCC 1 cut(s) 970
BshNI GGYRCC 1 cut(s) 800
BsiEI CGRYCG 3 cut(s) 265, 439, 959
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 7 cut(s) 104, 233, 282, 339, 525, 818, 971
BslFI GGGAC 3 cut(s) 290, 374, 507
BslI CCNNNNNNNGG 4 cut(s) 288, 774, 810, 977
BsmAI GTCTC 2 cut(s) 100, 101
BsmFI GGGAC 3 cut(s) 290, 374, 507
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 1 cut(s) 970
Bso31I GGTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 480
Bsp13I TCCGGA 2 cut(s) 103, 232
Bsp143I GATC 5 cut(s) 124, 241, 367, 764, 785
BspACI CCGC 9 cut(s) 81, 185, 294, 358, 404, 435, 515, 751, 978
BspANI GGCC 1 cut(s) 970
BspEI TCCGGA 2 cut(s) 103, 232
BspFNI CGCG 3 cut(s) 197, 294, 435
BspLI GGNNCC 2 cut(s) 513, 802
BspMAI CTGCAG 2 cut(s) 137, 750
BspPI GGATC 4 cut(s) 119, 375, 759, 793
BspT107I GGYRCC 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 880
BsrFI RCCGGY 1 cut(s) 338
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 804, 937
BssMI GATC 5 cut(s) 124, 241, 367, 764, 785
BssNI GRCGYC 4 cut(s) 69, 171, 698, 801
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 479
Bst4CI ACNGT 5 cut(s) 263, 304, 560, 659, 960
Bst6I CTCTTC 3 cut(s) 616, 660, 686
BstACI GRCGYC 4 cut(s) 69, 171, 698, 801
BstAPI GCANNNNNTGC 1 cut(s) 754
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 1 cut(s) 381
BstF5I GGATG 4 cut(s) 70, 199, 283, 844
BstFNI CGCG 3 cut(s) 197, 294, 435
BstH2I RGCGCY 2 cut(s) 804, 937
BstHHI GCGC 4 cut(s) 199, 294, 803, 936
BstKTI GATC 5 cut(s) 127, 244, 370, 767, 788
BstMAI GTCTC 2 cut(s) 100, 101
BstMBI GATC 5 cut(s) 124, 241, 367, 764, 785
BstMCI CGRYCG 3 cut(s) 265, 439, 959
BstMWI GCNNNNNNNGC 5 cut(s) 141, 745, 754, 757, 800
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 2 cut(s) 133, 746
BstUI CGCG 3 cut(s) 197, 294, 435
BstV1I GCAGC 6 cut(s) 147, 150, 153, 732, 744, 760
BstV2I GAAGAC 3 cut(s) 166, 198, 624
BstX2I RGATCY 2 cut(s) 124, 367
BstYI RGATCY 2 cut(s) 124, 367
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 1 cut(s) 970
BtgZI GCGATG 2 cut(s) 407, 579
BtsCI GGATG 4 cut(s) 70, 199, 283, 844
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 333, 664
Cac8I GCNNGC 1 cut(s) 780
CfoI GCGC 4 cut(s) 199, 294, 803, 936
Cfr10I RCCGGY 1 cut(s) 338
CseI GACGC 2 cut(s) 203, 706
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 943
CviJI RGCY 8 cut(s) 161, 425, 528, 604, 739, 745, 817, 970
CviKI_1 RGCY 8 cut(s) 161, 425, 528, 604, 739, 745, 817, 970
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 381
DinI GGCGCC 1 cut(s) 802
DpnI GATC 5 cut(s) 126, 243, 369, 766, 787
DpnII GATC 5 cut(s) 124, 241, 367, 764, 785
DraIII CACNNNGTG 1 cut(s) 484
DriI GACNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 968
Eam1104I CTCTTC 3 cut(s) 616, 660, 686
Eam1105I GACNNNNNGTC 1 cut(s) 40
EarI CTCTTC 3 cut(s) 616, 660, 686
Eco130I CCWWGG 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 710, 875
EcoRI GAATTC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 804
EgeI GGCGCC 1 cut(s) 802
EheI GGCGCC 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 804
FaeI CATG 1 cut(s) 946
FaiI YATR 8 cut(s) 92, 519, 627, 638, 686, 833, 867, 944
FaqI GGGAC 3 cut(s) 290, 374, 507
FatI CATG 1 cut(s) 942
FauI CCCGC 5 cut(s) 192, 351, 397, 744, 971
Fnu4HI GCNGC 7 cut(s) 136, 139, 142, 295, 746, 749, 758
FokI GGATG 4 cut(s) 77, 186, 290, 851
Fsp4HI GCNGC 7 cut(s) 136, 139, 142, 295, 746, 749, 758
FspBI CTAG 1 cut(s) 371
GlaI GCGC 4 cut(s) 198, 293, 802, 935
GluI GCNGC 7 cut(s) 136, 139, 142, 295, 746, 749, 758
HaeII RGCGCY 2 cut(s) 804, 937
HaeIII GGCC 1 cut(s) 970
HapII CCGG 7 cut(s) 104, 233, 282, 339, 525, 818, 971
HgaI GACGC 2 cut(s) 203, 706
HhaI GCGC 4 cut(s) 199, 294, 803, 936
Hin1I GRCGYC 4 cut(s) 69, 171, 698, 801
Hin1II CATG 1 cut(s) 946
Hin6I GCGC 4 cut(s) 197, 292, 801, 934
HinP1I GCGC 4 cut(s) 197, 292, 801, 934
HinfI GANTC 5 cut(s) 17, 38, 544, 608, 644
HpaII CCGG 7 cut(s) 104, 233, 282, 339, 525, 818, 971
HphI GGTGA 2 cut(s) 64, 607
Hpy188I TCNGA 9 cut(s) 115, 214, 246, 313, 475, 616, 655, 855, 986
Hpy188III TCNNGA 7 cut(s) 32, 104, 178, 233, 371, 548, 680
Hpy99I CGWCG 5 cut(s) 38, 46, 122, 254, 964
HpyAV CCTTC 3 cut(s) 31, 850, 968
HpyCH4III ACNGT 5 cut(s) 263, 304, 560, 659, 960
HpyCH4IV ACGT 2 cut(s) 69, 171
HpyCH4V TGCA 4 cut(s) 135, 724, 748, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 745, 754, 757, 800
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 2 cut(s) 69, 171
Hsp92I GRCGYC 4 cut(s) 69, 171, 698, 801
Hsp92II CATG 1 cut(s) 946
HspAI GCGC 4 cut(s) 197, 292, 801, 934
KasI GGCGCC 1 cut(s) 800
Kpn2I TCCGGA 2 cut(s) 103, 232
Kzo9I GATC 5 cut(s) 124, 241, 367, 764, 785
Lsp1109I GCAGC 6 cut(s) 147, 150, 153, 732, 744, 760
LweI GCATC 3 cut(s) 208, 268, 733
MaeI CTAG 1 cut(s) 371
MaeII ACGT 2 cut(s) 69, 171
MaeIII GTNAC 5 cut(s) 70, 298, 412, 530, 706
MalI GATC 5 cut(s) 126, 243, 369, 766, 787
MboI GATC 5 cut(s) 124, 241, 367, 764, 785
MboII GAAGA 9 cut(s) 134, 166, 203, 469, 603, 629, 653, 677, 703
MflI RGATCY 2 cut(s) 124, 367
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 8 cut(s) 55, 399, 419, 461, 491, 497, 552, 719
Mly113I GGCGCC 1 cut(s) 801
MlyI GAGTC 3 cut(s) 32, 553, 602
MroI TCCGGA 2 cut(s) 103, 232
MroXI GAANNNNTTC 2 cut(s) 510, 675
MseI TTAA 2 cut(s) 444, 501
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 7 cut(s) 104, 233, 282, 339, 525, 818, 971
MspR9I CCNGG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 675
MvnI CGCG 3 cut(s) 197, 294, 435
MwoI GCNNNNNNNGC 5 cut(s) 141, 745, 754, 757, 800
NarI GGCGCC 1 cut(s) 801
NciI CCSGG 1 cut(s) 525
NdeII GATC 5 cut(s) 124, 241, 367, 764, 785
NlaIII CATG 1 cut(s) 946
NlaIV GGNNCC 2 cut(s) 513, 802
NmeAIII GCCGAG 1 cut(s) 962
NmuCI GTSAC 3 cut(s) 70, 412, 706
PcsI WCGNNNNNNNCGW 3 cut(s) 12, 30, 246
PctI GAATGC 1 cut(s) 675
PdmI GAANNNNTTC 2 cut(s) 510, 675
PfeI GAWTC 2 cut(s) 17, 644
PkrI GCNGC 7 cut(s) 137, 140, 143, 296, 747, 750, 759
PleI GAGTC 3 cut(s) 32, 552, 602
PluTI GGCGCC 1 cut(s) 804
PpsI GAGTC 3 cut(s) 32, 552, 602
PshAI GACNNNNGTC 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 513, 802
PstI CTGCAG 2 cut(s) 137, 750
PsuI RGATCY 2 cut(s) 124, 367
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 444, 501
SatI GCNGC 7 cut(s) 136, 139, 142, 295, 746, 749, 758
Sau3AI GATC 5 cut(s) 124, 241, 367, 764, 785
SchI GAGTC 3 cut(s) 32, 553, 602
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 1 cut(s) 480
SetI ASST 7 cut(s) 54, 72, 174, 212, 486, 606, 828
SfaNI GCATC 3 cut(s) 208, 268, 733
SfcI CTRYAG 2 cut(s) 133, 746
SfoI GGCGCC 1 cut(s) 802
Sse9I AATT 8 cut(s) 55, 399, 419, 461, 491, 497, 552, 719
SsiI CCGC 9 cut(s) 81, 185, 294, 358, 404, 435, 515, 751, 978
SspDI GGCGCC 1 cut(s) 800
SspI AATATT 1 cut(s) 588
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 1 cut(s) 804
TaaI ACNGT 5 cut(s) 263, 304, 560, 659, 960
TaiI ACGT 2 cut(s) 72, 174
TaqI TCGA 7 cut(s) 24, 240, 273, 439, 575, 727, 763
TasI AATT 8 cut(s) 55, 399, 419, 461, 491, 497, 552, 719
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 2 cut(s) 17, 644
Tru1I TTAA 2 cut(s) 444, 501
Tru9I TTAA 2 cut(s) 444, 501
TscAI CASTG 2 cut(s) 340, 664
TseFI GTSAC 3 cut(s) 70, 412, 706
TseI GCWGC 6 cut(s) 135, 138, 141, 745, 748, 757
Tsp45I GTSAC 3 cut(s) 70, 412, 706
TspGWI ACGGA 5 cut(s) 216, 258, 341, 380, 431
TspRI CASTG 2 cut(s) 340, 664
XapI RAATTY 2 cut(s) 55, 497
XbaI TCTAGA 1 cut(s) 370
XmnI GAANNNNTTC 2 cut(s) 510, 675
XspI CTAG 1 cut(s) 371
ZraI GACGTC 2 cut(s) 70, 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.