pycom04g08670

Glycogen recognition site of AMP-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005701
Physical Location & Seq
Reverse (-)
10570190 .. 10572946
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 990 bp
ATGCCTGTAGAACCTGCTGATGGTTTGTCATTGGATGAAAAGGTCTTAGATATATGTCAGGATGGGAAGGTTAACAGTATGACTGATGAAAATTCCTCTTCAGCCGTAGTTTCCGGTTTGGAAGACCATTCTAGTTCAAATCTTGAATCAAGTCACAACTCTGATTACCATTACCCGCCTTTAAAATCTCCACCCAATTTATCCTTAGAGGAAAAAGTGGCAAACTTTATGCAGAGTGGAGACTTGGATGCAGTTGAAGTAACTGAAAATAATGCTGAAGAAATCAAAGGTAAATTTGGAAACACTGAAGAGGTTCAATTAAGAACTCCTACTTCGGGGCACTCTAAAAATGTGTTTGATGGAAGGGATGCAACTATGGCATTGAATCAAAGGACATCAACATCTACTATGGCAGTTGATCCCTCTCTTTGTAGGGATGATGGTACACCAGCTGAAGGGCTAAGTTCTCTATATGACAAGGATTTGGATGTTAAGACAAATGAAAGAGACTATGAACTCGATGCTAGTCATCTCAAGTTTATGCTGCATCAGAAGGAGATTGAATTGTCACAGTTGAAGGAACAGATTAAGAAAGAACAGCTTGCTTTGTCAAACTTGCAAACCAAGGCTGAAATGGCAATCAGCAAAGCACAAAGGCTTGTCTCTGAAAAAGATGCAGAGTTACTTGCTGCTGAAGAAAGCCTTTCAGGATTAGTGGAGGTTGAGATCCAGTACGAGGGAGATGGTGAGATTGTGGAGGTGACTGGTAGCTTCAATGGTTGGCATCATCAGATTAAAATGGATCTGGAGTCATCATCTAGTATCATAGAACCCTTGAGGAAACCCAAACTTTGGTCAACAATGCTGTGGCTTTATCCAGGGACATATGAGATAAAATTCATTGTTGATGGCCAATGGAAGATTGATCCTCAAAGAGAGTCAGTTACCAGGGGTACCATATGTAACAACATTCTCCGCGTTGACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMPK1_CBM

Protein Analysis

330

Amino Acids

36.68

Weight (kDa)

4.65

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 240 - 327 4.9e-18 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 22
Acc65I GGTACC 1 cut(s) 953
AccB1I GGYRCC 1 cut(s) 953
AccB7I CCANNNNNTGG 1 cut(s) 852
AccII CGCG 1 cut(s) 978
AciI CCGC 2 cut(s) 176, 976
AclWI GGATC 4 cut(s) 413, 721, 810, 920
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 3 cut(s) 91, 293, 896
AcuI CTGAAG 5 cut(s) 84, 297, 327, 474, 714
AfaI GTAC 3 cut(s) 445, 734, 955
AfiI CCNNNNNNNGG 5 cut(s) 20, 335, 455, 736, 852
AgsI TTSAA 8 cut(s) 138, 146, 257, 317, 385, 563, 577, 775
AjnI CCWGG 2 cut(s) 877, 947
AluBI AGCT 3 cut(s) 452, 601, 771
AluI AGCT 3 cut(s) 452, 601, 771
Alw26I GTCTC 3 cut(s) 234, 501, 667
AlwI GGATC 4 cut(s) 413, 721, 810, 920
AoxI GGCC 1 cut(s) 910
ApeKI GCWGC 2 cut(s) 544, 689
ApoI RAATTY 3 cut(s) 91, 293, 896
Asp700I GAANNNNTTC 1 cut(s) 312
Asp718I GGTACC 1 cut(s) 953
AsuHPI GGTGA 2 cut(s) 758, 772
BaeGI GKGCMC 1 cut(s) 342
BalI TGGCCA 1 cut(s) 912
BanI GGYRCC 1 cut(s) 953
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 2 cut(s) 531, 676
BccI CCATC 6 cut(s) 14, 56, 353, 434, 737, 902
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 2 cut(s) 879, 949
BcoDI GTCTC 3 cut(s) 234, 501, 667
BfaI CTAG 3 cut(s) 132, 525, 819
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 2 cut(s) 545, 690
BlsI GCNGC 2 cut(s) 546, 691
Bme1390I CCNGG 2 cut(s) 879, 949
BmiI GGNNCC 1 cut(s) 955
BmrFI CCNGG 2 cut(s) 879, 949
BmsI GCATC 6 cut(s) 238, 358, 511, 556, 664, 793
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 827
BpuEI CTTGAG 2 cut(s) 518, 856
BsaJI CCNNGG 3 cut(s) 624, 878, 948
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 335, 455, 736, 852
Bse1I ACTGG 2 cut(s) 730, 769
BseBI CCWGG 2 cut(s) 879, 949
BseDI CCNNGG 3 cut(s) 624, 878, 948
BseGI GGATG 6 cut(s) 40, 67, 253, 373, 442, 493
BseLI CCNNNNNNNGG 5 cut(s) 20, 335, 455, 736, 852
BseNI ACTGG 2 cut(s) 730, 769
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 2 cut(s) 531, 676
Bsh1236I CGCG 1 cut(s) 978
BshFI GGCC 1 cut(s) 912
BshNI GGYRCC 1 cut(s) 953
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 1 cut(s) 895
BslI CCNNNNNNNGG 5 cut(s) 20, 335, 455, 736, 852
BsmAI GTCTC 3 cut(s) 234, 501, 667
BsmFI GGGAC 1 cut(s) 895
BsnI GGCC 1 cut(s) 912
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 4 cut(s) 418, 726, 802, 925
BspACI CCGC 2 cut(s) 176, 976
BspANI GGCC 1 cut(s) 912
BspFNI CGCG 1 cut(s) 978
BspLI GGNNCC 1 cut(s) 955
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 4 cut(s) 413, 721, 810, 920
BspT107I GGYRCC 1 cut(s) 953
BsrI ACTGG 2 cut(s) 730, 769
BssECI CCNNGG 3 cut(s) 624, 878, 948
BssMI GATC 4 cut(s) 418, 726, 802, 925
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 2 cut(s) 879, 949
Bst4CI ACNGT 2 cut(s) 77, 573
Bst6I CTCTTC 2 cut(s) 103, 303
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 3 cut(s) 46, 205, 461
BstF5I GGATG 6 cut(s) 40, 67, 253, 373, 442, 493
BstFNI CGCG 1 cut(s) 978
BstKTI GATC 4 cut(s) 421, 729, 805, 928
BstMAI GTCTC 3 cut(s) 234, 501, 667
BstMBI GATC 4 cut(s) 418, 726, 802, 925
BstMWI GCNNNNNNNGC 2 cut(s) 377, 635
BstNI CCWGG 2 cut(s) 879, 949
BstSCI CCNGG 2 cut(s) 877, 947
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 342
BstUI CGCG 1 cut(s) 978
BstV1I GCAGC 2 cut(s) 531, 676
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 726, 802
BstYI RGATCY 2 cut(s) 726, 802
BsuRI GGCC 1 cut(s) 912
BtsCI GGATG 6 cut(s) 40, 67, 253, 373, 442, 493
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 603
Csp6I GTAC 3 cut(s) 444, 733, 954
CviQI GTAC 3 cut(s) 444, 733, 954
DdeI CTNAG 3 cut(s) 46, 205, 461
DpnI GATC 4 cut(s) 420, 728, 804, 927
DpnII GATC 4 cut(s) 418, 726, 802, 925
DraI TTTAAA 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 910
Eam1104I CTCTTC 2 cut(s) 103, 303
EarI CTCTTC 2 cut(s) 103, 303
Eco130I CCWWGG 1 cut(s) 624
Eco57I CTGAAG 5 cut(s) 84, 297, 327, 474, 714
EcoRII CCWGG 2 cut(s) 877, 947
EcoT14I CCWWGG 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 624
FalI AAGNNNNNCTT 4 cut(s) 585, 617, 687, 719
FaqI GGGAC 1 cut(s) 895
FauI CCCGC 1 cut(s) 183
FauNDI CATATG 2 cut(s) 886, 959
Fnu4HI GCNGC 2 cut(s) 545, 690
FokI GGATG 6 cut(s) 47, 74, 260, 380, 449, 500
Fsp4HI GCNGC 2 cut(s) 545, 690
FspBI CTAG 3 cut(s) 132, 525, 819
GluI GCNGC 2 cut(s) 545, 690
GsuI CTGGAG 1 cut(s) 827
HaeIII GGCC 1 cut(s) 912
HapII CCGG 1 cut(s) 114
HincII GTYRAC 3 cut(s) 73, 858, 982
HindII GTYRAC 3 cut(s) 73, 858, 982
HinfI GANTC 4 cut(s) 146, 385, 809, 938
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 1 cut(s) 114
HphI GGTGA 2 cut(s) 758, 772
Hpy166II GTNNAC 4 cut(s) 73, 446, 858, 982
Hpy188I TCNGA 4 cut(s) 163, 552, 667, 792
Hpy188III TCNNGA 4 cut(s) 59, 143, 708, 806
Hpy8I GTNNAC 4 cut(s) 73, 446, 858, 982
HpyAV CCTTC 5 cut(s) 61, 357, 449, 547, 571
HpyCH4III ACNGT 2 cut(s) 77, 573
HpyCH4V TGCA 6 cut(s) 232, 251, 371, 547, 619, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 635
HpyF3I CTNAG 3 cut(s) 46, 205, 461
KpnI GGTACC 1 cut(s) 957
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 418, 726, 802, 925
Lsp1109I GCAGC 2 cut(s) 531, 676
LweI GCATC 6 cut(s) 238, 358, 511, 556, 664, 793
MaeI CTAG 3 cut(s) 132, 525, 819
MaeIII GTNAC 7 cut(s) 152, 259, 567, 681, 760, 943, 962
MalI GATC 4 cut(s) 420, 728, 804, 927
MboI GATC 4 cut(s) 418, 726, 802, 925
MboII GAAGA 6 cut(s) 90, 134, 290, 320, 707, 931
MflI RGATCY 2 cut(s) 726, 802
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 912
MluCI AATT 6 cut(s) 91, 196, 293, 317, 563, 896
MluNI TGGCCA 1 cut(s) 912
MlyI GAGTC 2 cut(s) 818, 947
MnlI CCTC 9 cut(s) 106, 202, 304, 433, 712, 730, 751, 831, 939
Mox20I TGGCCA 1 cut(s) 912
MroXI GAANNNNTTC 1 cut(s) 312
MscI TGGCCA 1 cut(s) 912
MseI TTAA 6 cut(s) 72, 182, 320, 492, 588, 795
Msp20I TGGCCA 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 2 cut(s) 879, 949
MvaI CCWGG 2 cut(s) 879, 949
MvnI CGCG 1 cut(s) 978
MwoI GCNNNNNNNGC 2 cut(s) 377, 635
NdeI CATATG 2 cut(s) 886, 959
NdeII GATC 4 cut(s) 418, 726, 802, 925
NlaIV GGNNCC 1 cut(s) 955
NmuCI GTSAC 3 cut(s) 152, 567, 760
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 146, 385
PflMI CCANNNNNTGG 1 cut(s) 852
PkrI GCNGC 2 cut(s) 546, 691
PleI GAGTC 2 cut(s) 817, 946
PpsI GAGTC 2 cut(s) 817, 946
Psp6I CCWGG 2 cut(s) 877, 947
PspGI CCWGG 2 cut(s) 877, 947
PspN4I GGNNCC 1 cut(s) 955
PsuI RGATCY 2 cut(s) 726, 802
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 445, 734, 955
RsaNI GTAC 3 cut(s) 444, 733, 954
SaqAI TTAA 6 cut(s) 72, 182, 320, 492, 588, 795
SatI GCNGC 2 cut(s) 545, 690
Sau3AI GATC 4 cut(s) 418, 726, 802, 925
SchI GAGTC 2 cut(s) 818, 947
ScrFI CCNGG 2 cut(s) 879, 949
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 6 cut(s) 238, 358, 511, 556, 664, 793
SfcI CTRYAG 1 cut(s) 6
SmlI CTYRAG 2 cut(s) 533, 835
SmoI CTYRAG 2 cut(s) 533, 835
Sse9I AATT 6 cut(s) 91, 196, 293, 317, 563, 896
SsiI CCGC 2 cut(s) 176, 976
SspMI CTAG 3 cut(s) 132, 525, 819
StyD4I CCNGG 2 cut(s) 877, 947
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 2 cut(s) 77, 573
TaqI TCGA 1 cut(s) 519
TasI AATT 6 cut(s) 91, 196, 293, 317, 563, 896
TfiI GAWTC 2 cut(s) 146, 385
Tru1I TTAA 6 cut(s) 72, 182, 320, 492, 588, 795
Tru9I TTAA 6 cut(s) 72, 182, 320, 492, 588, 795
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 3 cut(s) 152, 567, 760
TseI GCWGC 2 cut(s) 544, 689
Tsp45I GTSAC 3 cut(s) 152, 567, 760
TspDTI ATGAA 5 cut(s) 51, 102, 516, 528, 889
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 852
XapI RAATTY 3 cut(s) 91, 293, 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 3 cut(s) 132, 525, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.