pycom04g09140

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002662
Physical Location & Seq
Reverse (-)
11492853 .. 11496962
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2781 bp
ATGGAATCCACCAATCCTAATGCTAACATTATGCCGGGTGTTCTACAGCGGTTGCTTCCTGTTGTTGGACCTGTGCTTCTGATTTCAGTTGGATATATTGACCCTGGAAAGTGGGCAGCAACTGCTGAAGCAGGTGCCCGTTTTGGCTCTGACCTCGCAGCATTGATGCTTATTTTCAATTTTGCAGCTATTTTATGTCACTATTTGTCAGCTCGGATTGGTGTAGTCACCGGAAGAGATCTTGCACAGATATGCAGTGAGGAGTATGACAAGGGCACATGCATATCCTTAGGAATTCAAACAGAGGTTTCTGCAATTCTGTCAGACCTAACCATGATCCTCGGCATCGCACATGGGCTTAATCTTCTGTTTGGTTGGGACTTGTTCACTTGTGTCTTTTTGACTGCTGTTAATGCCATTTTATACCCTCTTCTTTCCTCCTTCCTGGAAACTTGCAAGGCGAAGGTCCTATGCATATATGTTGCAGGATTCATACTCCTTTCCTTTGTTCTTGGAGTAATTATCAGTCAACCAGAAATGCCCCGTTCCATGAATGGGATGCTAACAAAGCTGAGTGGGGAGAGTGCATTTGCACTGATGAGTCTTCTTGGTGCAAGCATAATGCCTCACAGTCTTTATTTTCATTCTTCTATAGTGCAGCAATATCAGCAGCAAGCAACTGTTCCCAAGGGTGCTTTGTGTCATAACCATTTAGTTGCCATAGTATGCATCTTCAGTGGTGTTTATATGGTGAATTATGTCCTTATGACCTCAGCGGAAAATTTATTCTACAGTCCAGAGGCAAGTTCTGGCCTTGTTGTACTTGCGTTTCAGGATGCAATGTCGCTAATGGAACAGGTCTTTTGGGGTCCAATAGTACCGGCTTCCTTCTTACTGGTTCTCATTCTGTCAAATCAAATCACAACATTAAGCTGGAGTCTGGGTGGAGATGTAGTCCTGAATGAATTTTTGAAAGTGGACCTTCCTGGTTGGCTTCATTGCGCTACTATCAGAATTATTGCCATTGTTCCAGCCCTTTATTTTGTTTGGAGTTCAGGAGCCGAAGGAATGTATCAACTGCTTATATTCACACAAGTTTTGGCAGCTCTGCTACTACCATCTTCCGTGATCCCTATTTTTCGAATTGCTGCTTCAAGACCAATAATGGGTGCCCATAAAATTTCACAATTTGTTGAATTCTTATCCCTGATTACTCTTATTGGGATGCTTGGATTGAAAATTATATTTGTAGTAGAAATGATTTTTGGGAATAGTGAATGGGGTGGTAATTTGAGGTCGCATGGCGGGAGTAGTATGTTACTATTCCTCACTGCTTGTGCATCATTTGGTTTGATGATTTGGCTAGCAGCTACCCCGTTAAAATCTGCAAGTGTCCGATTAGAGGCTCAGGTGTGGAACTGGGAACTGATGGAGGGTACACCAGATTCATTGACAAATGAAGAAGATATTAACAGAGCTGAACCTCAATATCATAGAGAGGCAAGTGTCCAGAGCCATGAACCATCACCATCACTTGGGTTGGATGCTGATTCAGAAGTTGATAATTTTGATCTGGATCTGCCTGATACTATCATGGAGGCTGATCAGGAACTTCACCAAACAACTGTAGCAGAGAATAATAATTCTCATATTACCTTTCCTTGCTCCCCTAAAAGCCGCATGGAGGGATCCACATCTGCAGTGGAGTCATTTCCAGTCTCAACTGTTGTTAATGATGTTTCTGATCTCACATTGGAGGACACCAGTACACTGAAGTTCAAGTCAACAGAGTCAATTGAGAAGACTGTTGAAGCTGAAGCAGACTTACCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAGCCTGAAGAATCATCAAAAGGGGTTTCTGGGAGCACTGCGCCATTGGCCTCTGAGGGCTCGGGATCATTTAGGAGTCTAAGTGGAAAAGGTGATGATGGAGGAAGTAGTGCAGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCAAGACGTCAACTGGCTGCTGTGCTTGATGAATTTTGGGGACAACTGTATGATTTCCATGGAAATGTAATTCAAGACGCAAAGGCTAAAAAGCTGGACCTTCTGTTGGGATCAGATTCAAAGTCTGTGTCGTCCTCGCTGAAAGTTGATACCAGTCCAAAGGGAACTTCTGGATTCTTTCCATCCTCAGTAGGCAGAGGATCTGATTCTCTAATCAGCTCAAGTTTATATGAGTCTGCAAAGCAGCATAGGGTTCAAAGCAGTTTAGAGTCATATGTGGTCCAAAGGGGATCTTCCTCATTGTTGCCTAGCCACATGCAGTTGTTAGACACCTATGTGCAAAATTCAAGCCGCAGCATTATTGGCTCTGGTGAGAGGCGCTATTCAAGTGTGCGCAGTGTCCCAACTTCTGAGAGTGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATCCCAACTTCTGAGAGTTGGGATTACCAGCCAGCCATAGTACATGATTATCAGATTGCATCATATCTCAATCAAATGGCAAATGACAAAAGTTCTGATAACTTGAATGGTCAAATGGAGCCATCAGTCCTACAGTCCGGTTCTTCGTTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGATCTGGTCAGGCCTCCAGTTTTCAGAACTTTACAGTATCTAGAAATAGTCCAATGCAATCTGAAAGACCGAATCATGATCGATCCACGGTCTTCTGGAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

927

Amino Acids

100.19

Weight (kDa)

4.93

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 395 5.5e-80 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
AatII GACGTC 1 cut(s) 2023
Acc16I TGCGCA 2 cut(s) 2408, 2456
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 134, 1169
AccB7I CCANNNNNTGG 1 cut(s) 1535
AciI CCGC 5 cut(s) 49, 776, 1305, 1678, 2365
AcsI RAATTY 7 cut(s) 294, 781, 965, 1179, 1196, 2045, 2356
AcuI CTGAAG 5 cut(s) 147, 718, 1793, 1836, 1890
AcyI GRCGYC 1 cut(s) 2020
AfaI GTAC 5 cut(s) 822, 879, 1438, 1768, 2502
AfiI CCNNNNNNNGG 7 cut(s) 65, 555, 1166, 1402, 1535, 1684, 2119
AjnI CCWGG 4 cut(s) 103, 444, 985, 1859
AjuI GAANNNNNNNTTGG 4 cut(s) 1248, 1280, 2164, 2196
AleI CACNNNNGTG 1 cut(s) 2348
Alw21I GWGCWC 1 cut(s) 1901
Alw26I GTCTC 1 cut(s) 1723
AlwNI CAGNNNCTG 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 1924
AoxI GGCC 3 cut(s) 811, 1911, 2685
ApoI RAATTY 7 cut(s) 294, 781, 965, 1179, 1196, 2045, 2356
ArsI GACNNNNNNTTYG 2 cut(s) 2126, 2158
Asp700I GAANNNNTTC 1 cut(s) 1984
AspLEI GCGC 6 cut(s) 1004, 1906, 2394, 2409, 2442, 2457
AspS9I GGNCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 7 cut(s) 220, 763, 1518, 1607, 1967, 2396, 2444
AsuII TTCGAA 1 cut(s) 1141
AsuNHI GCTAGC 1 cut(s) 1363
AvaI CYCGRG 1 cut(s) 1924
AvaII GGWCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
AxyI CCTNAGG 1 cut(s) 289
BaeGI GKGCMC 3 cut(s) 139, 278, 1174
BamHI GGATCC 1 cut(s) 1688
BanI GGYRCC 2 cut(s) 134, 1169
BanII GRGCYC 1 cut(s) 1925
BarI GAAGNNNNNNTAC 4 cut(s) 1809, 1841, 2452, 2484
BbsI GAAGAC 3 cut(s) 596, 1808, 2758
Bbv12I GWGCWC 1 cut(s) 1901
BbvCI CCTCAGC 1 cut(s) 772
BccI CCATC 7 cut(s) 1126, 1423, 1531, 1537, 1955, 2203, 2590
BciT130I CCWGG 4 cut(s) 105, 446, 987, 1861
BciVI GTATCC 1 cut(s) 2471
BclI TGATCA 1 cut(s) 1603
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 1723
BfaI CTAG 3 cut(s) 1364, 2322, 2715
BfmI CTRYAG 7 cut(s) 44, 651, 790, 1626, 1698, 2591, 2623
BfoI RGCGCY 2 cut(s) 2395, 2443
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 1 cut(s) 2471
BglII AGATCT 1 cut(s) 238
Bme1390I CCNGG 5 cut(s) 36, 105, 446, 987, 1861
Bme18I GGWCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
BmeT110I CYCGRG 1 cut(s) 1924
BmgT120I GGNCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
BmiI GGNNCC 7 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2580
BmrFI CCNGG 5 cut(s) 36, 105, 446, 987, 1861
BmrI ACTGGG 1 cut(s) 1429
BmsI GCATC 9 cut(s) 156, 354, 549, 738, 826, 1215, 1349, 1534, 2528
BmtI GCTAGC 1 cut(s) 1367
BmuI ACTGGG 1 cut(s) 1429
BpiI GAAGAC 3 cut(s) 596, 1808, 2758
BpmI CTGGAG 2 cut(s) 955, 2674
Bpu10I CCTNAGC 2 cut(s) 772, 1407
Bpu14I TTCGAA 1 cut(s) 1141
BpuEI CTTGAG 1 cut(s) 2218
BpuMI CCSGG 1 cut(s) 36
Bsa29I ATCGAT 1 cut(s) 2755
BsaBI GATNNNNATC 2 cut(s) 1575, 1693
BsaHI GRCGYC 1 cut(s) 2020
BsaJI CCNNGG 7 cut(s) 103, 340, 687, 1860, 2071, 2646, 2760
BsaWI WCCGGW 2 cut(s) 230, 2597
BsaXI ACNNNNNCTCC 6 cut(s) 928, 939, 958, 969, 1930, 1960
Bsc4I CCNNNNNNNGG 7 cut(s) 65, 555, 1166, 1402, 1535, 1684, 2119
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 7 cut(s) 900, 1424, 1715, 1764, 2031, 2166, 2691
Bse21I CCTNAGG 1 cut(s) 289
Bse3DI GCAATG 2 cut(s) 846, 997
Bse8I GATNNNNATC 2 cut(s) 1575, 1693
BseBI CCWGG 4 cut(s) 105, 446, 987, 1861
BseCI ATCGAT 1 cut(s) 2755
BseDI CCNNGG 7 cut(s) 103, 340, 687, 1860, 2071, 2646, 2760
BseGI GGATG 5 cut(s) 564, 841, 1230, 1549, 2195
BseJI GATNNNNATC 2 cut(s) 1575, 1693
BseLI CCNNNNNNNGG 7 cut(s) 65, 555, 1166, 1402, 1535, 1684, 2119
BseMI GCAATG 2 cut(s) 846, 997
BseMII CTCAG 7 cut(s) 563, 786, 1421, 1908, 2214, 2415, 2463
BseNI ACTGG 7 cut(s) 900, 1424, 1715, 1764, 2031, 2166, 2691
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 3 cut(s) 139, 278, 1174
BsgI GTGCAG 2 cut(s) 677, 1995
BshFI GGCC 3 cut(s) 813, 1913, 2687
BshNI GGYRCC 2 cut(s) 134, 1169
BshVI ATCGAT 1 cut(s) 2755
BsiHKAI GWGCWC 1 cut(s) 1901
BsiHKCI CYCGRG 1 cut(s) 1924
BsiSI CCGG 4 cut(s) 35, 231, 881, 2598
BslFI GGGAC 3 cut(s) 392, 2067, 2399
BslI CCNNNNNNNGG 7 cut(s) 65, 555, 1166, 1402, 1535, 1684, 2119
BsmAI GTCTC 1 cut(s) 1723
BsmFI GGGAC 3 cut(s) 392, 2067, 2399
BsnI GGCC 3 cut(s) 813, 1913, 2687
BsoBI CYCGRG 1 cut(s) 1924
Bsp119I TTCGAA 1 cut(s) 1141
Bsp1286I GDGCHC 5 cut(s) 139, 278, 1174, 1901, 1925
Bsp19I CCATGG 2 cut(s) 2071, 2646
BspACI CCGC 5 cut(s) 49, 776, 1305, 1678, 2365
BspANI GGCC 3 cut(s) 813, 1913, 2687
BspCNI CTCAG 7 cut(s) 564, 785, 1420, 1909, 2213, 2416, 2464
BspDI ATCGAT 1 cut(s) 2755
BspHI TCATGA 1 cut(s) 2749
BspLI GGNNCC 7 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2580
BspMAI CTGCAG 1 cut(s) 1702
BspMI ACCTGC 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 1367
BspT104I TTCGAA 1 cut(s) 1141
BspT107I GGYRCC 2 cut(s) 134, 1169
BsrDI GCAATG 2 cut(s) 846, 997
BsrFI RCCGGY 1 cut(s) 880
BsrI ACTGG 7 cut(s) 900, 1424, 1715, 1764, 2031, 2166, 2691
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 7 cut(s) 103, 340, 687, 1860, 2071, 2646, 2760
BssNI GRCGYC 1 cut(s) 2020
BssT1I CCWWGG 3 cut(s) 687, 2071, 2646
Bst2UI CCWGG 4 cut(s) 105, 446, 987, 1861
Bst6I CTCTTC 2 cut(s) 229, 435
BstACI GRCGYC 1 cut(s) 2020
BstAPI GCANNNNNTGC 2 cut(s) 122, 2659
BstBI TTCGAA 1 cut(s) 1141
BstC8I GCNNGC 5 cut(s) 616, 675, 1365, 2010, 2493
BstDEI CTNAG 9 cut(s) 289, 572, 772, 1407, 1917, 1943, 2200, 2424, 2472
BstDSI CCRYGG 3 cut(s) 2071, 2646, 2760
BstF5I GGATG 5 cut(s) 564, 841, 1230, 1549, 2195
BstH2I RGCGCY 2 cut(s) 2395, 2443
BstHHI GCGC 6 cut(s) 1004, 1906, 2394, 2409, 2442, 2457
BstMAI GTCTC 1 cut(s) 1723
BstMWI GCNNNNNNNGC 7 cut(s) 122, 413, 568, 667, 1910, 2376, 2659
BstNI CCWGG 4 cut(s) 105, 446, 987, 1861
BstNSI RCATGY 2 cut(s) 282, 2332
BstSCI CCNGG 5 cut(s) 34, 103, 444, 985, 1859
BstSFI CTRYAG 7 cut(s) 44, 651, 790, 1626, 1698, 2591, 2623
BstSLI GKGCMC 3 cut(s) 139, 278, 1174
BstV2I GAAGAC 3 cut(s) 596, 1808, 2758
BstX2I RGATCY 6 cut(s) 238, 1576, 1688, 2213, 2303, 2675
BstYI RGATCY 6 cut(s) 238, 1576, 1688, 2213, 2303, 2675
Bsu15I ATCGAT 1 cut(s) 2755
Bsu36I CCTNAGG 1 cut(s) 289
BsuI GTATCC 1 cut(s) 2471
BsuRI GGCC 3 cut(s) 813, 1913, 2687
BsuTUI ATCGAT 1 cut(s) 2755
BtgI CCRYGG 3 cut(s) 2071, 2646, 2760
BtgZI GCGATG 1 cut(s) 331
BtsCI GGATG 5 cut(s) 564, 841, 1230, 1549, 2195
BtsI GCAGTG 5 cut(s) 262, 1329, 1707, 1899, 2416
BtsIMutI CAGTG 8 cut(s) 262, 593, 742, 1329, 1707, 1769, 1899, 2416
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 5 cut(s) 616, 675, 1365, 2010, 2493
CaiI CAGNNNCTG 1 cut(s) 122
CciI TCATGA 1 cut(s) 2749
CfoI GCGC 6 cut(s) 1004, 1906, 2394, 2409, 2442, 2457
Cfr10I RCCGGY 1 cut(s) 880
Cfr13I GGNCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
ClaI ATCGAT 1 cut(s) 2755
CseI GACGC 1 cut(s) 2099
Csp6I GTAC 5 cut(s) 821, 878, 1437, 1767, 2501
CviQI GTAC 5 cut(s) 821, 878, 1437, 1767, 2501
DdeI CTNAG 9 cut(s) 289, 572, 772, 1407, 1917, 1943, 2200, 2424, 2472
Eam1104I CTCTTC 2 cut(s) 229, 435
EarI CTCTTC 2 cut(s) 229, 435
Eco130I CCWWGG 3 cut(s) 687, 2071, 2646
Eco147I AGGCCT 1 cut(s) 2687
Eco24I GRGCYC 1 cut(s) 1925
Eco47I GGWCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
Eco57I CTGAAG 5 cut(s) 147, 718, 1793, 1836, 1890
Eco81I CCTNAGG 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 1924
EcoO109I RGGNCCY 1 cut(s) 466
EcoRI GAATTC 2 cut(s) 294, 1196
EcoRII CCWGG 4 cut(s) 103, 444, 985, 1859
EcoT14I CCWWGG 3 cut(s) 687, 2071, 2646
EcoT22I ATGCAT 3 cut(s) 284, 476, 731
EcoT38I GRGCYC 1 cut(s) 1925
ErhI CCWWGG 3 cut(s) 687, 2071, 2646
FalI AAGNNNNNCTT 4 cut(s) 966, 998, 2290, 2322
FaqI GGGAC 3 cut(s) 392, 2067, 2399
FauI CCCGC 1 cut(s) 1298
FauNDI CATATG 1 cut(s) 2287
FbaI TGATCA 1 cut(s) 1603
FokI GGATG 5 cut(s) 571, 848, 1237, 1556, 2182
FriOI GRGCYC 1 cut(s) 1925
FspBI CTAG 3 cut(s) 1364, 2322, 2715
FspI TGCGCA 2 cut(s) 2408, 2456
GlaI GCGC 6 cut(s) 1003, 1905, 2393, 2408, 2441, 2456
GsuI CTGGAG 2 cut(s) 955, 2674
HaeII RGCGCY 2 cut(s) 2395, 2443
HaeIII GGCC 3 cut(s) 813, 1913, 2687
HapII CCGG 4 cut(s) 35, 231, 881, 2598
HgaI GACGC 1 cut(s) 2099
HhaI GCGC 6 cut(s) 1004, 1906, 2394, 2409, 2442, 2457
Hin1I GRCGYC 1 cut(s) 2020
Hin6I GCGC 6 cut(s) 1002, 1904, 2392, 2407, 2440, 2455
HinP1I GCGC 6 cut(s) 1002, 1904, 2392, 2407, 2440, 2455
HincII GTYRAC 3 cut(s) 530, 1785, 2024
HindII GTYRAC 3 cut(s) 530, 1785, 2024
HpaII CCGG 4 cut(s) 35, 231, 881, 2598
HphI GGTGA 7 cut(s) 220, 763, 1518, 1607, 1967, 2396, 2444
Hpy166II GTNNAC 7 cut(s) 387, 530, 979, 1439, 1769, 1785, 2024
Hpy8I GTNNAC 7 cut(s) 387, 530, 979, 1439, 1769, 1785, 2024
HpyAV CCTTC 6 cut(s) 451, 457, 898, 992, 1058, 2123
HpyCH4IV ACGT 1 cut(s) 2020
HpyF10VI GCNNNNNNNGC 7 cut(s) 122, 413, 568, 667, 1910, 2376, 2659
HpyF3I CTNAG 9 cut(s) 289, 572, 772, 1407, 1917, 1943, 2200, 2424, 2472
HpySE526I ACGT 1 cut(s) 2020
Hsp92I GRCGYC 1 cut(s) 2020
HspAI GCGC 6 cut(s) 1002, 1904, 2392, 2407, 2440, 2455
Ksp22I TGATCA 1 cut(s) 1603
LmnI GCTCC 5 cut(s) 1058, 1670, 1864, 1896, 2578
LweI GCATC 9 cut(s) 156, 354, 549, 738, 826, 1215, 1349, 1534, 2528
MaeI CTAG 3 cut(s) 1364, 2322, 2715
MaeII ACGT 1 cut(s) 2020
MaeIII GTNAC 3 cut(s) 197, 226, 1317
MfeI CAATTG 1 cut(s) 1794
MflI RGATCY 6 cut(s) 238, 1576, 1688, 2213, 2303, 2675
MhlI GDGCHC 5 cut(s) 139, 278, 1174, 1901, 1925
MlyI GAGTC 7 cut(s) 610, 946, 1715, 1799, 1948, 2255, 2291
MmeI TCCRAC 3 cut(s) 46, 70, 1521
Mph1103I ATGCAT 3 cut(s) 284, 476, 731
MroXI GAANNNNTTC 1 cut(s) 1984
MseI TTAA 6 cut(s) 360, 411, 929, 1379, 1470, 1731
MslI CAYNNNNRTG 3 cut(s) 250, 2076, 2348
MspA1I CMGCKG 2 cut(s) 49, 776
MspI CCGG 4 cut(s) 35, 231, 881, 2598
MspR9I CCNGG 5 cut(s) 36, 105, 446, 987, 1861
MunI CAATTG 1 cut(s) 1794
MvaI CCWGG 4 cut(s) 105, 446, 987, 1861
MwoI GCNNNNNNNGC 7 cut(s) 122, 413, 568, 667, 1910, 2376, 2659
NciI CCSGG 1 cut(s) 36
NcoI CCATGG 2 cut(s) 2071, 2646
NdeI CATATG 1 cut(s) 2287
NheI GCTAGC 1 cut(s) 1363
NlaIV GGNNCC 7 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2580
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 197, 226
NsbI TGCGCA 2 cut(s) 2408, 2456
NsiI ATGCAT 3 cut(s) 284, 476, 731
NspI RCATGY 2 cut(s) 282, 2332
NspV TTCGAA 1 cut(s) 1141
OliI CACNNNNGTG 1 cut(s) 2348
PagI TCATGA 1 cut(s) 2749
PaqCI CACCTGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 2687
PdmI GAANNNNTTC 1 cut(s) 1984
PflMI CCANNNNNTGG 1 cut(s) 1535
PfoI TCCNGGA 1 cut(s) 444
PleI GAGTC 7 cut(s) 609, 945, 1714, 1798, 1947, 2254, 2290
PpsI GAGTC 7 cut(s) 609, 945, 1714, 1798, 1947, 2254, 2290
PpuMI RGGWCCY 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 4 cut(s) 103, 444, 985, 1859
PspGI CCWGG 4 cut(s) 103, 444, 985, 1859
PspN4I GGNNCC 7 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2580
PspPI GGNCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
PspPPI RGGWCCY 1 cut(s) 466
PstI CTGCAG 1 cut(s) 1702
PstNI CAGNNNCTG 1 cut(s) 122
PsuI RGATCY 6 cut(s) 238, 1576, 1688, 2213, 2303, 2675
RsaI GTAC 5 cut(s) 822, 879, 1438, 1768, 2502
RsaNI GTAC 5 cut(s) 821, 878, 1437, 1767, 2501
RseI CAYNNNNRTG 3 cut(s) 250, 2076, 2348
SaqAI TTAA 6 cut(s) 360, 411, 929, 1379, 1470, 1731
Sau96I GGNCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
SchI GAGTC 7 cut(s) 610, 946, 1715, 1799, 1948, 2255, 2291
ScrFI CCNGG 5 cut(s) 36, 105, 446, 987, 1861
SduI GDGCHC 5 cut(s) 139, 278, 1174, 1901, 1925
SfaNI GCATC 9 cut(s) 156, 354, 549, 738, 826, 1215, 1349, 1534, 2528
SfcI CTRYAG 7 cut(s) 44, 651, 790, 1626, 1698, 2591, 2623
SfuI TTCGAA 1 cut(s) 1141
SinI GGWCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
SmiMI CAYNNNNRTG 3 cut(s) 250, 2076, 2348
SmlI CTYRAG 1 cut(s) 2233
SmoI CTYRAG 1 cut(s) 2233
SseBI AGGCCT 1 cut(s) 2687
SsiI CCGC 5 cut(s) 49, 776, 1305, 1678, 2365
SspMI CTAG 3 cut(s) 1364, 2322, 2715
StuI AGGCCT 1 cut(s) 2687
StyD4I CCNGG 5 cut(s) 34, 103, 444, 985, 1859
StyI CCWWGG 3 cut(s) 687, 2071, 2646
TaiI ACGT 1 cut(s) 2023
TaqI TCGA 2 cut(s) 1141, 2755
TaqII GACCGA 1 cut(s) 2758
TatI WGTACW 3 cut(s) 820, 1766, 2500
TauI GCSGC 2 cut(s) 1680, 2367
Tru1I TTAA 6 cut(s) 360, 411, 929, 1379, 1470, 1731
Tru9I TTAA 6 cut(s) 360, 411, 929, 1379, 1470, 1731
TscAI CASTG 8 cut(s) 262, 600, 742, 1336, 1707, 1776, 1906, 2416
TseFI GTSAC 2 cut(s) 197, 226
Tsp45I GTSAC 2 cut(s) 197, 226
TspDTI ATGAA 9 cut(s) 481, 566, 632, 978, 986, 1437, 1473, 1533, 2058
TspGWI ACGGA 1 cut(s) 1114
TspRI CASTG 8 cut(s) 262, 600, 742, 1336, 1707, 1776, 1906, 2416
Van91I CCANNNNNTGG 1 cut(s) 1535
VpaK11BI GGWCC 6 cut(s) 68, 466, 869, 979, 2110, 2293
XapI RAATTY 7 cut(s) 294, 781, 965, 1179, 1196, 2045, 2356
XbaI TCTAGA 1 cut(s) 2714
XceI RCATGY 2 cut(s) 282, 2332
XcmI CCANNNNNNNNNTGG 1 cut(s) 1699
XmnI GAANNNNTTC 1 cut(s) 1984
XspI CTAG 3 cut(s) 1364, 2322, 2715
ZraI GACGTC 1 cut(s) 2021
Zsp2I ATGCAT 3 cut(s) 284, 476, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.