pycom04g09370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012636
Physical Location & Seq
Forward (+)
11961866 .. 11962606
741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09370.1

Sequence Viewer

Length: 741 bp
ATGGCAATATCTGAGTTCTCCAAAATATGCATGATCTGCATAGTCACATTGCTCTCTGTGGCAATGGCTTCACAACAATCCCCACAACCCTCATTCAAGATTGACACCATCCCTGCAGATGATGAGCTGATTTCCCTGGCTGCTGCTTCGTCTCCATCACAATCTCCTTATGCCGAAAATCTGGCTCCCGAAACTGGAGGCTATTTTGCCCCTTCAACAGATTCAGATCAATACAATACTCCACCGCAACCATCACAACCTTATATTGAGACATTTGCCCCTGAAGCTCAAGAGCTTCTCTCTCCAGACTACTCTCCTTTCCCTGCTCCTACTTACTTCCAAGATTACCCTTCTCCTCCTCCTTACATTGAAGCTCAGGCTCCTGAAAGTTACTATGATTTTTACAGTCCCATTCAAGGTCCAAGTCCAGCACCATCTTCCGAACGGGGATCTTCTCAATTTTCGGATACTGATGATTTCACTTCTCCTCCAAACTTGGCTCCTCAGCCCAATGTGTTGCCTTCCAACTACAGAGCCGAGGATGAGATTGGTGAGCTTGGTGAGCTAGAATCTGATGAAGCTTATAACTCTGACCAGAATAAGAATAGGGCAACAATGGCGGGTTTTGTGCTTGGGTCTGTTTGCTTGGTTGGATTGGCAGGTTTTGTGTACAACAAGAAGAAGTATAGCAAGTCAAAAGCAGATTATGAGTATGAATTGGCTAAGAGAGAGGATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.88

Weight (kDa)

4.05

Isoelectric Point (pI)

77.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
Acc36I ACCTGC 1 cut(s) 650
AciI CCGC 2 cut(s) 245, 620
AclWI GGATC 1 cut(s) 457
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 1 cut(s) 671
AfiI CCNNNNNNNGG 2 cut(s) 194, 416
AgsI TTSAA 4 cut(s) 97, 216, 371, 416
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 7 cut(s) 127, 287, 295, 374, 556, 565, 581
AluI AGCT 7 cut(s) 127, 287, 295, 374, 556, 565, 581
Alw26I GTCTC 2 cut(s) 156, 263
AlwI GGATC 1 cut(s) 457
ApeKI GCWGC 2 cut(s) 140, 143
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 2 cut(s) 563, 572
AvaII GGWCC 1 cut(s) 419
BbvCI CCTCAGC 1 cut(s) 504
BbvI GCAGC 2 cut(s) 127, 130
BccI CCATC 4 cut(s) 116, 163, 259, 442
BciT130I CCWGG 1 cut(s) 137
BciVI GTATCC 1 cut(s) 460
BcoDI GTCTC 2 cut(s) 156, 263
BfaI CTAG 2 cut(s) 566, 739
BfmI CTRYAG 2 cut(s) 114, 529
BfuAI ACCTGC 1 cut(s) 650
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 2 cut(s) 141, 144
BlsI GCNGC 2 cut(s) 142, 145
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 3 cut(s) 186, 381, 501
BmrFI CCNGG 1 cut(s) 137
BplI GAGNNNNNCTC 3 cut(s) 284, 316, 720
BpmI CTGGAG 2 cut(s) 216, 288
Bpu10I CCTNAGC 2 cut(s) 375, 504
BpuEI CTTGAG 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 135, 537
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 416
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 2 cut(s) 47, 69
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 135, 537
BseGI GGATG 2 cut(s) 108, 547
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 194, 416
BseMI GCAATG 2 cut(s) 47, 69
BseMII CTCAG 2 cut(s) 389, 518
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 4 cut(s) 345, 348, 477, 492
BseXI GCAGC 2 cut(s) 127, 130
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 2 cut(s) 194, 416
BsmAI GTCTC 2 cut(s) 156, 263
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 393
Bsp1407I TGTACA 1 cut(s) 669
Bsp143I GATC 4 cut(s) 33, 226, 449, 733
BspACI CCGC 2 cut(s) 245, 620
BspCNI CTCAG 3 cut(s) 4, 388, 517
BspLI GGNNCC 3 cut(s) 186, 381, 501
BspMAI CTGCAG 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 1 cut(s) 457
BsrDI GCAATG 2 cut(s) 47, 69
BsrGI TGTACA 1 cut(s) 669
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 135, 537
BssMI GATC 4 cut(s) 33, 226, 449, 733
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 407
BstAPI GCANNNNNTGC 1 cut(s) 36
BstAUI TGTACA 1 cut(s) 669
BstDEI CTNAG 4 cut(s) 12, 375, 504, 723
BstF5I GGATG 2 cut(s) 108, 547
BstKTI GATC 4 cut(s) 36, 229, 452, 736
BstMAI GTCTC 2 cut(s) 156, 263
BstMBI GATC 4 cut(s) 33, 226, 449, 733
BstMWI GCNNNNNNNGC 4 cut(s) 36, 284, 562, 617
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 2 cut(s) 114, 529
BstV1I GCAGC 2 cut(s) 127, 130
BstX2I RGATCY 2 cut(s) 449, 733
BstYI RGATCY 2 cut(s) 449, 733
BsuI GTATCC 1 cut(s) 460
BtsCI GGATG 2 cut(s) 108, 547
BveI ACCTGC 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 419
Csp6I GTAC 1 cut(s) 670
CviAII CATG 1 cut(s) 31
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 4 cut(s) 12, 375, 504, 723
DpnI GATC 4 cut(s) 35, 228, 451, 735
DpnII GATC 4 cut(s) 33, 226, 449, 733
Eco47I GGWCC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 32
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 1 cut(s) 34
FaqI GGGAC 1 cut(s) 393
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 613
Fnu4HI GCNGC 2 cut(s) 141, 144
FokI GGATG 2 cut(s) 95, 554
Fsp4HI GCNGC 2 cut(s) 141, 144
FspBI CTAG 2 cut(s) 566, 739
GluI GCNGC 2 cut(s) 141, 144
GsuI CTGGAG 2 cut(s) 216, 288
Hin1II CATG 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 2 cut(s) 221, 569
HphI GGTGA 2 cut(s) 563, 572
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 6 cut(s) 13, 226, 442, 466, 574, 592
Hpy188III TCNNGA 5 cut(s) 97, 188, 290, 305, 383
Hpy8I GTNNAC 1 cut(s) 670
HpyAV CCTTC 3 cut(s) 222, 360, 531
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4V TGCA 3 cut(s) 30, 39, 116
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 284, 562, 617
HpyF3I CTNAG 4 cut(s) 12, 375, 504, 723
Hsp92II CATG 1 cut(s) 34
Kzo9I GATC 4 cut(s) 33, 226, 449, 733
LmnI GCTCC 4 cut(s) 190, 331, 385, 505
Lsp1109I GCAGC 2 cut(s) 127, 130
MaeI CTAG 2 cut(s) 566, 739
MaeIII GTNAC 2 cut(s) 43, 389
MalI GATC 4 cut(s) 35, 228, 451, 735
MboI GATC 4 cut(s) 33, 226, 449, 733
MboII GAAGA 3 cut(s) 429, 444, 691
MflI RGATCY 2 cut(s) 449, 733
MluCI AATT 2 cut(s) 458, 716
MmeI TCCRAC 2 cut(s) 549, 631
MnlI CCTC 8 cut(s) 100, 191, 366, 369, 498, 513, 532, 724
Mph1103I ATGCAT 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 4 cut(s) 36, 284, 562, 617
NdeII GATC 4 cut(s) 33, 226, 449, 733
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 3 cut(s) 186, 381, 501
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 32
PfeI GAWTC 2 cut(s) 221, 569
PkrI GCNGC 2 cut(s) 142, 145
PsiI TTATAA 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 3 cut(s) 186, 381, 501
PspPI GGNCC 1 cut(s) 419
PstI CTGCAG 1 cut(s) 118
PsuI RGATCY 2 cut(s) 449, 733
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
SatI GCNGC 2 cut(s) 141, 144
Sau3AI GATC 4 cut(s) 33, 226, 449, 733
Sau96I GGNCC 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 137
SfcI CTRYAG 2 cut(s) 114, 529
SinI GGWCC 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
Sse9I AATT 2 cut(s) 458, 716
SsiI CCGC 2 cut(s) 245, 620
SspMI CTAG 2 cut(s) 566, 739
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 407
TasI AATT 2 cut(s) 458, 716
TatI WGTACW 1 cut(s) 669
TfiI GAWTC 2 cut(s) 221, 569
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 140, 143
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 591, 729
VpaK11BI GGWCC 1 cut(s) 419
XspI CTAG 2 cut(s) 566, 739
Zsp2I ATGCAT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.