pycom04g17500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007714
Physical Location & Seq
Forward (+)
19866621 .. 19868565
1945 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17500.1

Sequence Viewer

Length: 849 bp
ATGAACTTCGATCACTTGCCTGAAGACTGCTGCGCGCACATTTTATCGTTCACGTCTCCGGGCGACGCATGTCGTTCCTCGTTAGTTTCATCGTCTTTTCGGGCCACGGCGGATGCCGATAGTGTGTGGCGGAAATTCTTGCCATCAGACCACAAACAGATTCTATCCAGATTTGTGAGTCCCATAGCTTATTCATGTAGCAAAGATTTGTTTATGAAGTTATGCAGTCCAAACCTAATCGACGGTGGTGATAAGATGTTCTTCATAGAGAAATCAACAGGTAAAAAGTGCTACATGTTAAGTGCAAGGGACCTTTCAATTACATGGGGCAGTCATCCACTGTACTGGACCTGGAGACCTTGTCTTGAATCAAGATTTGCAGAGGTGGCTGAGCTTAGAACAATTTGGTGGCTGGAAATATGTGGCACAACAAATACTCAAATGCTATCACCAAAAACTGCCTATGGGGCCTATCTTATTATAAAGATAGCCAATCGTGCTTACGGGTTAGACACTTTGCCTTCTGAGGTTTCACTTGAAGTTGGTAATTCAAAATCACAAAGTACGATTTACTTGAGCAAACGCAATGATAGTGGGAAGCAAGCCTCATCAGAACACGAACATTTTCCGAAAGCGGTAAGAGCTTCAAGGTGGAGGGTTCTAGACGAAGATCGCAGTGGCGGCCGCGGCGGCGAGCGCGGGGATGGATGGATGGAGGTTGAAATAGGAAGCTTTTACAATGGTGAGTGTGATGAGAAGGATGTAAGGATGAGCCTAAGAGAAGTGAAGGGTGTGCATCTTAAAGGTGGACTTATTGTTGAGGGAATTGAGCTTAGGCCTAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

283

Amino Acids

31.72

Weight (kDa)

7.56

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 3 - 46 5.9e-09 F-box-like
F-box PF00646 3 - 45 4.5e-07 F-box domain
PP2 PF14299 95 - 280 5.2e-54 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AccII CGCG 3 cut(s) 35, 687, 699
AciI CCGC 8 cut(s) 110, 130, 635, 681, 685, 687, 690, 699
AcoI YGGCCR 1 cut(s) 682
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 344, 565
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 6 cut(s) 318, 368, 539, 552, 648, 722
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 1 cut(s) 350
AluBI AGCT 5 cut(s) 188, 394, 644, 732, 832
AluI AGCT 5 cut(s) 188, 394, 644, 732, 832
Alw26I GTCTC 2 cut(s) 60, 349
AoxI GGCC 4 cut(s) 102, 468, 682, 836
ApeKI GCWGC 1 cut(s) 30
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 624
AspLEI GCGC 3 cut(s) 35, 37, 699
AspS9I GGNCC 4 cut(s) 102, 310, 348, 468
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 260, 441, 755
AvaII GGWCC 2 cut(s) 310, 348
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 151, 698, 702, 706
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 1 cut(s) 352
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 60, 349
BfaI CTAG 1 cut(s) 662
BglI GCCNNNNNGGC 2 cut(s) 467, 690
BisI GCNGC 5 cut(s) 31, 682, 685, 688, 691
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 5 cut(s) 32, 683, 686, 689, 692
Bme1390I CCNGG 2 cut(s) 60, 352
Bme18I GGWCC 2 cut(s) 310, 348
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 4 cut(s) 102, 310, 348, 468
BmiI GGNNCC 2 cut(s) 311, 469
BmrFI CCNGG 2 cut(s) 60, 352
BmsI GCATC 2 cut(s) 103, 805
BoxI GACNNNNGTC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 373
Bpu10I CCTNAGC 1 cut(s) 833
Bpu1102I GCTNAGC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 105, 685
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bse1I ACTGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 592
BseBI CCWGG 1 cut(s) 352
BseDI CCNNGG 2 cut(s) 105, 685
BseGI GGATG 7 cut(s) 118, 334, 709, 713, 717, 766, 774
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 2 cut(s) 381, 516
BseNI ACTGG 1 cut(s) 350
BsePI GCGCGC 1 cut(s) 33
BseX3I CGGCCG 1 cut(s) 682
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 3 cut(s) 35, 687, 699
Bsh1285I CGRYCG 1 cut(s) 685
BshFI GGCC 4 cut(s) 104, 470, 684, 838
BsiEI CGRYCG 1 cut(s) 685
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 165, 323
BsmAI GTCTC 2 cut(s) 60, 349
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 2 cut(s) 165, 323
BsnI GGCC 4 cut(s) 104, 470, 684, 838
Bso31I GGTCTC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 10, 670
Bsp1720I GCTNAGC 1 cut(s) 390
BspACI CCGC 8 cut(s) 110, 130, 635, 681, 685, 687, 690, 699
BspANI GGCC 4 cut(s) 104, 470, 684, 838
BspCNI CTCAG 2 cut(s) 382, 517
BspFNI CGCG 3 cut(s) 35, 687, 699
BspLI GGNNCC 2 cut(s) 311, 469
BspTNI GGTCTC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 1 cut(s) 350
BssECI CCNNGG 2 cut(s) 105, 685
BssHII GCGCGC 1 cut(s) 33
BssMI GATC 2 cut(s) 10, 670
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 2 cut(s) 245, 342
BstC8I GCNNGC 3 cut(s) 35, 603, 695
BstDEI CTNAG 5 cut(s) 390, 395, 525, 776, 833
BstDSI CCRYGG 2 cut(s) 105, 685
BstF5I GGATG 7 cut(s) 118, 334, 709, 713, 717, 766, 774
BstFNI CGCG 3 cut(s) 35, 687, 699
BstHHI GCGC 3 cut(s) 35, 37, 699
BstKTI GATC 2 cut(s) 13, 673
BstMAI GTCTC 2 cut(s) 60, 349
BstMBI GATC 2 cut(s) 10, 670
BstMCI CGRYCG 1 cut(s) 685
BstMWI GCNNNNNNNGC 8 cut(s) 386, 467, 497, 641, 681, 687, 690, 696
BstNI CCWGG 1 cut(s) 352
BstNSI RCATGY 2 cut(s) 72, 298
BstPAI GACNNNNGTC 1 cut(s) 69
BstSCI CCNGG 2 cut(s) 58, 350
BstUI CGCG 3 cut(s) 35, 687, 699
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 345
BstZI CGGCCG 1 cut(s) 682
BsuRI GGCC 4 cut(s) 104, 470, 684, 838
BtgI CCRYGG 2 cut(s) 105, 685
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 7 cut(s) 118, 334, 709, 713, 717, 766, 774
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 2 cut(s) 338, 682
Cac8I GCNNGC 3 cut(s) 35, 603, 695
CciNI GCGGCCGC 1 cut(s) 682
CfoI GCGC 3 cut(s) 35, 37, 699
Cfr13I GGNCC 4 cut(s) 102, 310, 348, 468
Cfr42I CCGCGG 1 cut(s) 688
CseI GACGC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 343, 564
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 4 cut(s) 69, 195, 295, 324
CviQI GTAC 2 cut(s) 343, 564
DdeI CTNAG 5 cut(s) 390, 395, 525, 776, 833
DpnI GATC 2 cut(s) 12, 672
DpnII GATC 2 cut(s) 10, 670
EaeI YGGCCR 1 cut(s) 682
EagI CGGCCG 1 cut(s) 682
EciI GGCGGA 2 cut(s) 125, 145
EclXI CGGCCG 1 cut(s) 682
Eco147I AGGCCT 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 2 cut(s) 310, 348
Eco52I CGGCCG 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 2 cut(s) 310, 468
EcoRII CCWGG 1 cut(s) 350
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 4 cut(s) 72, 198, 298, 327
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 795, 827
FaqI GGGAC 2 cut(s) 165, 323
FatI CATG 4 cut(s) 68, 194, 294, 323
FauI CCCGC 1 cut(s) 692
Fnu4HI GCNGC 5 cut(s) 31, 682, 685, 688, 691
FokI GGATG 7 cut(s) 125, 321, 716, 720, 724, 773, 781
Fsp4HI GCNGC 5 cut(s) 31, 682, 685, 688, 691
FspBI CTAG 1 cut(s) 662
GlaI GCGC 3 cut(s) 34, 36, 698
GluI GCNGC 5 cut(s) 31, 682, 685, 688, 691
GsuI CTGGAG 1 cut(s) 373
HaeIII GGCC 4 cut(s) 104, 470, 684, 838
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 74
HhaI GCGC 3 cut(s) 35, 37, 699
Hin1II CATG 4 cut(s) 72, 198, 298, 327
Hin6I GCGC 3 cut(s) 33, 35, 697
HinP1I GCGC 3 cut(s) 33, 35, 697
HindIII AAGCTT 1 cut(s) 730
HinfI GANTC 3 cut(s) 160, 178, 368
HpaII CCGG 1 cut(s) 59
HphI GGTGA 3 cut(s) 260, 441, 755
Hpy166II GTNNAC 2 cut(s) 51, 809
Hpy188I TCNGA 4 cut(s) 148, 526, 613, 630
Hpy188III TCNNGA 4 cut(s) 168, 365, 372, 662
Hpy8I GTNNAC 2 cut(s) 51, 809
Hpy99I CGWCG 2 cut(s) 68, 245
HpyAV CCTTC 3 cut(s) 531, 751, 781
HpyCH4III ACNGT 2 cut(s) 245, 342
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 4 cut(s) 225, 305, 380, 796
HpyF10VI GCNNNNNNNGC 8 cut(s) 386, 467, 497, 641, 681, 687, 690, 696
HpyF3I CTNAG 5 cut(s) 390, 395, 525, 776, 833
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 4 cut(s) 72, 198, 298, 327
HspAI GCGC 3 cut(s) 33, 35, 697
KspI CCGCGG 1 cut(s) 688
Kzo9I GATC 2 cut(s) 10, 670
LpnPI CCDG 8 cut(s) 33, 72, 181, 264, 331, 337, 364, 398
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 2 cut(s) 103, 805
MaeI CTAG 1 cut(s) 662
MaeII ACGT 1 cut(s) 53
MalI GATC 2 cut(s) 12, 672
MboI GATC 2 cut(s) 10, 670
MboII GAAGA 3 cut(s) 35, 253, 680
MluCI AATT 5 cut(s) 134, 318, 402, 547, 825
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 7 cut(s) 88, 376, 520, 616, 648, 709, 814
MroXI GAANNNNTTC 1 cut(s) 624
MseI TTAA 2 cut(s) 299, 801
MspA1I CMGCKG 1 cut(s) 687
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 60, 352
MvaI CCWGG 1 cut(s) 352
MvnI CGCG 3 cut(s) 35, 687, 699
MwoI GCNNNNNNNGC 8 cut(s) 386, 467, 497, 641, 681, 687, 690, 696
NciI CCSGG 1 cut(s) 60
NdeII GATC 2 cut(s) 10, 670
NlaIII CATG 4 cut(s) 72, 198, 298, 327
NlaIV GGNNCC 2 cut(s) 311, 469
NotI GCGGCCGC 1 cut(s) 682
NspI RCATGY 2 cut(s) 72, 298
PauI GCGCGC 1 cut(s) 33
PceI AGGCCT 1 cut(s) 838
PciI ACATGT 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 624
PfeI GAWTC 2 cut(s) 160, 368
PflFI GACNNNGTC 1 cut(s) 360
PkrI GCNGC 5 cut(s) 32, 683, 686, 689, 692
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
PpuMI RGGWCCY 1 cut(s) 310
PscI ACATGT 1 cut(s) 294
PshAI GACNNNNGTC 1 cut(s) 69
PsiI TTATAA 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 2 cut(s) 311, 469
PspPI GGNCC 4 cut(s) 102, 310, 348, 468
PspPPI RGGWCCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 360
PteI GCGCGC 1 cut(s) 33
RsaI GTAC 2 cut(s) 344, 565
RsaNI GTAC 2 cut(s) 343, 564
SacII CCGCGG 1 cut(s) 688
SaqAI TTAA 2 cut(s) 299, 801
SatI GCNGC 5 cut(s) 31, 682, 685, 688, 691
Sau3AI GATC 2 cut(s) 10, 670
Sau96I GGNCC 4 cut(s) 102, 310, 348, 468
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 60, 352
SfaNI GCATC 2 cut(s) 103, 805
Sfr303I CCGCGG 1 cut(s) 688
SgrBI CCGCGG 1 cut(s) 688
SinI GGWCC 2 cut(s) 310, 348
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
Sse9I AATT 5 cut(s) 134, 318, 402, 547, 825
SseBI AGGCCT 1 cut(s) 838
SsiI CCGC 8 cut(s) 110, 130, 635, 681, 685, 687, 690, 699
SspMI CTAG 1 cut(s) 662
StuI AGGCCT 1 cut(s) 838
StyD4I CCNGG 2 cut(s) 58, 350
TaaI ACNGT 2 cut(s) 245, 342
TaiI ACGT 1 cut(s) 56
TaqI TCGA 2 cut(s) 9, 240
TasI AATT 5 cut(s) 134, 318, 402, 547, 825
TatI WGTACW 1 cut(s) 342
TauI GCSGC 4 cut(s) 684, 687, 690, 693
TfiI GAWTC 2 cut(s) 160, 368
Tru1I TTAA 2 cut(s) 299, 801
Tru9I TTAA 2 cut(s) 299, 801
TscAI CASTG 2 cut(s) 345, 682
TseI GCWGC 1 cut(s) 30
TspDTI ATGAA 5 cut(s) 17, 78, 183, 230, 253
TspRI CASTG 2 cut(s) 345, 682
Tth111I GACNNNGTC 1 cut(s) 360
VpaK11BI GGWCC 2 cut(s) 310, 348
XapI RAATTY 1 cut(s) 134
XbaI TCTAGA 1 cut(s) 661
XceI RCATGY 2 cut(s) 72, 298
XmnI GAANNNNTTC 1 cut(s) 624
XspI CTAG 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.