Rorug03G0012800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007367
Physical Location & Seq
Reverse (-)
1000614 .. 1002610
1997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0012800.1

Sequence Viewer

Length: 1020 bp
ATGCTGACACAAACACACGAAGGAAGAACGAGAACGAGAACAAGGCACCACGCAGTGAAACAGAGCAGCAAGAAAACCATGCGTAACGATGAAGAAGATGGACGAGGAGGAATCACCTGCGGCGCTGTGACGGTGGAGCTGGATCTGCAGGATTTGCCGGAGGGATGCATAGCCAACGTCATCTCCCTGACGACTCCTCCCGATGCGTGCCGGCTGTCGTCGGTTTCGAGGAGTTTCAGGTCGGCCGCCGAATCCGACGCCGTTTGGAGCAGGTTCCTGCCGCCGGAGATTCCCACGATCCTGTCTCACTCGGGGCCGCACGCCCCCAGTTGCCGATCCAAATCCAAGGAGTTCTACCTCGCCCTCTGCGACAATCCGGTGCTCATTGACGATGGCAAAATGAGCTTTTCACTAGACAAATGGAGTGGGAAAAAATGTTACATGGTATCTGCAAGAGACCTTGTGATTGTTTGGGCTGATACTCCTAACTATTGGAAATGGATTTCTCTGCCCGAGTCCAGCAAAAACAGATTTGAGGAGGTTGCTGAGCTTGTTGGTGTTTGCTGGCTTGAAATCCGTGGCAAAATTGACACTCGCTTGCTGTCCCCATCGACTGTGTACAAAGCTTACCTTGTGTTCAAGTCAACTGCAGCGGCTTATGGATTTGAATACCAACCTGCCGAGGTCAGTGTAGGTCTGGTTGGAGGAGCAGAACCCACAAAGAAGACTGTGTTTCTTGACGCCGATAGAGGCCGGACTCAGAGCTACCAGATTATGCCAAGGCGTGGCCCATTGAGAAACCGCATTTCGGTGCAAGTGTCCCAGCCCAGAGAGATCAATGACAATCAATACCCGAAGGAAAGAGCAGACGGGTGGTTGGAGATTGAGCTGGGTGAGTTCTTCTGTGAAGGAGGAGTAGATGGGGAGTTGGAAATGACTTGTTTGGAAACCAGGGGAGGTCAGTGGAAGGGTGGTCTTATTGTTCAAGCCATTGAGGTCAGGCCAAAGAGGAAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

339

Amino Acids

37.92

Weight (kDa)

8.01

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 49 - 90 7.7e-07 F-box domain
F-box-like PF12937 50 - 92 1.4e-06 F-box-like
PP2 PF14299 144 - 335 3.5e-56 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 125
Acc36I ACCTGC 3 cut(s) 125, 261, 685
AccB1I GGYRCC 1 cut(s) 45
AccB7I CCANNNNNTGG 1 cut(s) 785
AciI CCGC 6 cut(s) 120, 246, 281, 317, 653, 802
AclWI GGATC 3 cut(s) 150, 292, 330
AcoI YGGCCR 1 cut(s) 243
AcyI GRCGYC 2 cut(s) 258, 741
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 1 cut(s) 620
AfiI CCNNNNNNNGG 3 cut(s) 283, 785, 808
AgsI TTSAA 4 cut(s) 572, 640, 668, 986
AjnI CCWGG 1 cut(s) 950
AluBI AGCT 6 cut(s) 139, 405, 550, 626, 765, 889
AluI AGCT 6 cut(s) 139, 405, 550, 626, 765, 889
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 2 cut(s) 309, 450
AlwI GGATC 3 cut(s) 150, 292, 330
Ama87I CYCGRG 2 cut(s) 310, 512
AoxI GGCC 5 cut(s) 243, 314, 751, 787, 1001
ApeKI GCWGC 2 cut(s) 66, 650
ArsI GACNNNNNNTTYG 1 cut(s) 998
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 314, 788
AsuHPI GGTGA 2 cut(s) 106, 905
AvaI CYCGRG 2 cut(s) 310, 512
BanI GGYRCC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 78, 662
BccI CCATC 4 cut(s) 92, 386, 616, 914
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 952
BcoDI GTCTC 2 cut(s) 309, 450
BfaI CTAG 2 cut(s) 413, 1018
BfmI CTRYAG 2 cut(s) 146, 648
BfoI RGCGCY 1 cut(s) 126
BfuAI ACCTGC 3 cut(s) 125, 261, 685
BisI GCNGC 7 cut(s) 67, 121, 246, 281, 317, 651, 654
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 7 cut(s) 68, 122, 247, 282, 318, 652, 655
Bme1390I CCNGG 1 cut(s) 952
BmeT110I CYCGRG 2 cut(s) 310, 512
BmgT120I GGNCC 2 cut(s) 314, 788
BmiI GGNNCC 3 cut(s) 47, 275, 315
BmrFI CCNGG 1 cut(s) 952
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 155, 193
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 731
Bpu1102I GCTNAGC 1 cut(s) 546
BsaBI GATNNNNATC 1 cut(s) 340
BsaHI GRCGYC 2 cut(s) 258, 741
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 5 cut(s) 345, 577, 681, 779, 951
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 4 cut(s) 167, 181, 197, 211
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 785, 808
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 327
Bse8I GATNNNNATC 1 cut(s) 340
BseBI CCWGG 1 cut(s) 952
BseDI CCNNGG 5 cut(s) 345, 577, 681, 779, 951
BseGI GGATG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 283, 785, 808
BseMII CTCAG 2 cut(s) 537, 773
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 6 cut(s) 120, 186, 244, 551, 720, 927
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 78, 662
BseYI CCCAGC 2 cut(s) 822, 889
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 5 cut(s) 245, 316, 753, 789, 1003
BshNI GGYRCC 1 cut(s) 45
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 2 cut(s) 310, 512
BsiSI CCGG 5 cut(s) 158, 211, 284, 377, 754
BslFI GGGAC 2 cut(s) 589, 805
BslI CCNNNNNNNGG 3 cut(s) 283, 785, 808
BsmAI GTCTC 2 cut(s) 309, 450
BsmFI GGGAC 2 cut(s) 589, 805
BsnI GGCC 5 cut(s) 245, 316, 753, 789, 1003
Bso31I GGTCTC 1 cut(s) 450
BsoBI CYCGRG 2 cut(s) 310, 512
Bsp1286I GDGCHC 1 cut(s) 384
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 4 cut(s) 142, 297, 335, 834
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 6 cut(s) 120, 246, 281, 317, 653, 802
BspANI GGCC 5 cut(s) 245, 316, 753, 789, 1003
BspCNI CTCAG 2 cut(s) 538, 772
BspLI GGNNCC 3 cut(s) 47, 275, 315
BspMAI CTGCAG 2 cut(s) 150, 652
BspMI ACCTGC 3 cut(s) 125, 261, 685
BspPI GGATC 3 cut(s) 150, 292, 330
BspT107I GGYRCC 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 210
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 1 cut(s) 327
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 5 cut(s) 345, 577, 681, 779, 951
BssMI GATC 4 cut(s) 142, 297, 335, 834
BssNI GRCGYC 2 cut(s) 258, 741
BssT1I CCWWGG 2 cut(s) 345, 779
Bst2UI CCWGG 1 cut(s) 952
Bst4CI ACNGT 3 cut(s) 133, 616, 730
BstACI GRCGYC 2 cut(s) 258, 741
BstAPI GCANNNNNTGC 1 cut(s) 154
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 5 cut(s) 208, 212, 321, 566, 599
BstDEI CTNAG 2 cut(s) 546, 759
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 145, 300, 338, 837
BstMAI GTCTC 2 cut(s) 309, 450
BstMBI GATC 4 cut(s) 142, 297, 335, 834
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 3 cut(s) 145, 154, 402
BstNI CCWGG 1 cut(s) 952
BstSCI CCNGG 1 cut(s) 950
BstSFI CTRYAG 2 cut(s) 146, 648
BstV1I GCAGC 2 cut(s) 78, 662
BstV2I GAAGAC 1 cut(s) 731
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 5 cut(s) 245, 316, 753, 789, 1003
BtgI CCRYGG 1 cut(s) 577
BtsCI GGATG 1 cut(s) 170
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 3 cut(s) 60, 694, 968
BveI ACCTGC 3 cut(s) 125, 261, 685
Cac8I GCNNGC 5 cut(s) 208, 212, 321, 566, 599
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 314, 788
CseI GACGC 2 cut(s) 266, 749
Csp6I GTAC 1 cut(s) 619
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 2 cut(s) 79, 442
CviQI GTAC 1 cut(s) 619
DdeI CTNAG 2 cut(s) 546, 759
DpnI GATC 4 cut(s) 144, 299, 337, 836
DpnII GATC 4 cut(s) 142, 297, 335, 834
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 345, 779
Eco31I GGTCTC 1 cut(s) 450
Eco52I CGGCCG 1 cut(s) 243
Eco88I CYCGRG 2 cut(s) 310, 512
EcoRII CCWGG 1 cut(s) 950
EcoT14I CCWWGG 2 cut(s) 345, 779
EcoT22I ATGCAT 1 cut(s) 170
ErhI CCWWGG 2 cut(s) 345, 779
FaeI CATG 2 cut(s) 82, 445
FaiI YATR 5 cut(s) 80, 170, 443, 660, 776
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 2 cut(s) 589, 805
FatI CATG 2 cut(s) 78, 441
Fnu4HI GCNGC 7 cut(s) 67, 121, 246, 281, 317, 651, 654
FokI GGATG 1 cut(s) 177
Fsp4HI GCNGC 7 cut(s) 67, 121, 246, 281, 317, 651, 654
FspBI CTAG 2 cut(s) 413, 1018
GlaI GCGC 1 cut(s) 124
GluI GCNGC 7 cut(s) 67, 121, 246, 281, 317, 651, 654
GsaI CCCAGC 2 cut(s) 826, 893
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 5 cut(s) 245, 316, 753, 789, 1003
HapII CCGG 5 cut(s) 158, 211, 284, 377, 754
HgaI GACGC 2 cut(s) 266, 749
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 2 cut(s) 258, 741
Hin1II CATG 2 cut(s) 82, 445
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 6 cut(s) 111, 193, 251, 289, 515, 757
HpaII CCGG 5 cut(s) 158, 211, 284, 377, 754
HphI GGTGA 2 cut(s) 106, 905
Hpy166II GTNNAC 2 cut(s) 619, 645
Hpy188I TCNGA 2 cut(s) 256, 762
Hpy188III TCNNGA 2 cut(s) 200, 737
Hpy8I GTNNAC 2 cut(s) 619, 645
Hpy99I CGWCG 2 cut(s) 223, 260
HpyAV CCTTC 4 cut(s) 14, 850, 902, 961
HpyCH4III ACNGT 3 cut(s) 133, 616, 730
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 148, 168, 452, 650, 814
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 154, 402
HpyF3I CTNAG 2 cut(s) 546, 759
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 2 cut(s) 258, 741
Hsp92II CATG 2 cut(s) 82, 445
HspAI GCGC 1 cut(s) 123
KroI GCCGGC 1 cut(s) 210
KroNI GCCGGC 1 cut(s) 212
Kzo9I GATC 4 cut(s) 142, 297, 335, 834
LmnI GCTCC 3 cut(s) 136, 267, 707
Lsp1109I GCAGC 2 cut(s) 78, 662
LweI GCATC 2 cut(s) 155, 193
MaeI CTAG 2 cut(s) 413, 1018
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 83, 127, 437
MalI GATC 4 cut(s) 144, 299, 337, 836
MboI GATC 4 cut(s) 142, 297, 335, 834
MboII GAAGA 5 cut(s) 36, 104, 107, 736, 892
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 1 cut(s) 585
MlyI GAGTC 3 cut(s) 187, 524, 751
MmeI TCCRAC 4 cut(s) 279, 682, 858, 909
Mph1103I ATGCAT 1 cut(s) 170
MroNI GCCGGC 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 5 cut(s) 158, 211, 284, 377, 754
MspR9I CCNGG 1 cut(s) 952
MvaI CCWGG 1 cut(s) 952
MwoI GCNNNNNNNGC 3 cut(s) 145, 154, 402
NaeI GCCGGC 1 cut(s) 212
NdeII GATC 4 cut(s) 142, 297, 335, 834
NgoMIV GCCGGC 1 cut(s) 210
NlaIII CATG 2 cut(s) 82, 445
NlaIV GGNNCC 3 cut(s) 47, 275, 315
NmeAIII GCCGAG 1 cut(s) 706
NmuCI GTSAC 1 cut(s) 127
NsiI ATGCAT 1 cut(s) 170
PaqCI CACCTGC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 2 cut(s) 224, 366
PdiI GCCGGC 1 cut(s) 212
PfeI GAWTC 3 cut(s) 111, 251, 289
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 7 cut(s) 68, 122, 247, 282, 318, 652, 655
PleI GAGTC 3 cut(s) 187, 523, 751
PpsI GAGTC 3 cut(s) 187, 523, 751
Psp6I CCWGG 1 cut(s) 950
PspFI CCCAGC 2 cut(s) 822, 889
PspGI CCWGG 1 cut(s) 950
PspN4I GGNNCC 3 cut(s) 47, 275, 315
PspPI GGNCC 2 cut(s) 314, 788
PstI CTGCAG 2 cut(s) 150, 652
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 620
RsaNI GTAC 1 cut(s) 619
RseI CAYNNNNRTG 1 cut(s) 809
SatI GCNGC 7 cut(s) 67, 121, 246, 281, 317, 651, 654
Sau3AI GATC 4 cut(s) 142, 297, 335, 834
Sau96I GGNCC 2 cut(s) 314, 788
SchI GAGTC 3 cut(s) 187, 524, 751
ScrFI CCNGG 1 cut(s) 952
SduI GDGCHC 1 cut(s) 384
SfaNI GCATC 2 cut(s) 155, 193
SfcI CTRYAG 2 cut(s) 146, 648
SmiMI CAYNNNNRTG 1 cut(s) 809
Sse9I AATT 1 cut(s) 585
SsiI CCGC 6 cut(s) 120, 246, 281, 317, 653, 802
SspMI CTAG 2 cut(s) 413, 1018
StyD4I CCNGG 1 cut(s) 950
StyI CCWWGG 2 cut(s) 345, 779
TaaI ACNGT 3 cut(s) 133, 616, 730
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 227, 611
TasI AATT 1 cut(s) 585
TatI WGTACW 1 cut(s) 618
TauI GCSGC 5 cut(s) 123, 248, 283, 319, 656
TfiI GAWTC 3 cut(s) 111, 251, 289
TscAI CASTG 3 cut(s) 60, 694, 968
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 66, 650
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 105
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 3 cut(s) 60, 694, 968
Van91I CCANNNNNTGG 1 cut(s) 785
XspI CTAG 2 cut(s) 413, 1018
Zsp2I ATGCAT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.