MD04G1197600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007367
Physical Location & Seq
Reverse (-)
28557659 .. 28560472
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1197600.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGCGATAACCCACAACCTCAAACCCCCTATTTACATGCCTTCCCCTTCCGTTTCATCCAAAACCCATAATTCCTCGCTCTTATTTGGCACTGTTACTTCAACAGTTCACAGCCACGGAGTCCAGCCGCCGCCATTGGCATTCAGAAGTCGCACTGCTACTAACTCGCAAATCTTCAGCACCAGAAGACTCTTCCTGCCTAAGGTTTCCGGCATCTGGGATGCCTTGACTAGCGGCGGCAACAATCCCCGTGAAGCCGTTGCGGCGATTCGACGCGGGATGGTGCTTTTTAGGCAGGGTGATGTTTCAGGTTCACTAGCAGAATTTGACAAGGCAATTGAGTTGGATCCTCGCCAAAAGGCATATCTTTGGCAAAGGGGGCTTTCACTTTACTATCTTGATAGGTTTGAAGACGGGGCAGAGCAGTTTCGGTTAGATGTTGCGCAGAATCCAAATGATACAGAGGAGTCCATTTGGTGCTTTCTCTGTGAAGCTCAGTTGTATGGAGTTAACGAAGCAAGGGAACGATTTCTTGAGGTTGGCCGAGATCCAAGACCTGTCATGCGAGAATCCTATAACATGTTTAAAGATGGTGGTGATCCGGAAAAGCTTGCTGCTGCGTTTTTGAATGGCCGTGAGAGTGATTTTTTCTACGCTTCTTTATACGCCGGACTTTATTACGAATCTGAGAAGAAAGCCGATGGGGCCAAACTCCATATTGTTGCTGCATGCCAGTCTCTTTATGGACAAAGGTCCGACGATTACATGGCTTCTCTTGCCAAAGTTCACTGTCTTTGTCGAAATTGGACGGCCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

30.53

Weight (kDa)

7.01

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
AccII CGCG 1 cut(s) 276
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 6 cut(s) 128, 131, 234, 237, 263, 276
AclWI GGATC 4 cut(s) 341, 354, 542, 593
AcoI YGGCCR 3 cut(s) 541, 631, 810
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 202, 216
AflIII ACRYGT 1 cut(s) 579
AgsI TTSAA 3 cut(s) 102, 410, 628
AluBI AGCT 2 cut(s) 494, 610
AluI AGCT 2 cut(s) 494, 610
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 4 cut(s) 341, 354, 542, 593
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 4 cut(s) 541, 631, 705, 810
ApeKI GCWGC 3 cut(s) 614, 617, 725
ApoI RAATTY 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 528
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 2 cut(s) 705, 753
AsuHPI GGTGA 2 cut(s) 311, 608
AvaII GGWCC 1 cut(s) 753
AxyI CCTNAGG 1 cut(s) 201
BamHI GGATCC 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 193, 417
BbvI GCAGC 3 cut(s) 601, 604, 712
BccI CCATC 3 cut(s) 274, 584, 695
BceAI ACGGC 2 cut(s) 242, 618
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 2 cut(s) 231, 317
BglI GCCNNNNNGGC 2 cut(s) 263, 704
BisI GCNGC 8 cut(s) 128, 131, 235, 238, 264, 615, 618, 726
BlsI GCNGC 8 cut(s) 129, 132, 236, 239, 265, 616, 619, 727
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 2 cut(s) 705, 753
BmiI GGNNCC 2 cut(s) 348, 706
BmsI GCATC 2 cut(s) 211, 222
BoxI GACNNNNGTC 1 cut(s) 751
BpiI GAAGAC 2 cut(s) 193, 417
BpuEI CTTGAG 1 cut(s) 554
BsaJI CCNNGG 1 cut(s) 115
BsaWI WCCGGW 1 cut(s) 601
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 216
Bse1I ACTGG 2 cut(s) 733, 813
Bse21I CCTNAGG 1 cut(s) 201
BseAI TCCGGA 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 3 cut(s) 56, 226, 285
BseLI CCNNNNNNNGG 2 cut(s) 202, 216
BseMII CTCAG 2 cut(s) 509, 678
BseNI ACTGG 2 cut(s) 733, 813
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 3 cut(s) 601, 604, 712
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 543, 633, 707, 812
BsiSI CCGG 3 cut(s) 210, 602, 669
BslI CCNNNNNNNGG 2 cut(s) 202, 216
BsmAI GTCTC 1 cut(s) 741
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 4 cut(s) 543, 633, 707, 812
Bsp13I TCCGGA 1 cut(s) 601
Bsp143I GATC 3 cut(s) 346, 547, 598
BspACI CCGC 6 cut(s) 128, 131, 234, 237, 263, 276
BspANI GGCC 4 cut(s) 543, 633, 707, 812
BspCNI CTCAG 2 cut(s) 508, 679
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 348, 706
BspPI GGATC 4 cut(s) 341, 354, 542, 593
BsrI ACTGG 2 cut(s) 733, 813
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 3 cut(s) 346, 547, 598
Bst4CI ACNGT 3 cut(s) 94, 106, 791
Bst6I CTCTTC 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 612, 730
BstDEI CTNAG 3 cut(s) 201, 495, 687
BstDSI CCRYGG 1 cut(s) 115
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 56, 226, 285
BstFNI CGCG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 349, 550, 601
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 3 cut(s) 346, 547, 598
BstMWI GCNNNNNNNGC 5 cut(s) 263, 292, 379, 704, 776
BstNSI RCATGY 3 cut(s) 40, 583, 732
BstPAI GACNNNNGTC 1 cut(s) 751
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 601, 604, 712
BstV2I GAAGAC 2 cut(s) 193, 417
BstX2I RGATCY 2 cut(s) 346, 547
BstYI RGATCY 2 cut(s) 346, 547
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 4 cut(s) 543, 633, 707, 812
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 3 cut(s) 56, 226, 285
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 3 cut(s) 90, 153, 787
Cac8I GCNNGC 2 cut(s) 612, 730
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 2 cut(s) 705, 753
CseI GACGC 1 cut(s) 282
CviAII CATG 5 cut(s) 37, 562, 580, 729, 766
DdeI CTNAG 3 cut(s) 201, 495, 687
DpnI GATC 3 cut(s) 348, 549, 600
DpnII GATC 3 cut(s) 346, 547, 598
DraI TTTAAA 1 cut(s) 586
EaeI YGGCCR 3 cut(s) 541, 631, 810
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 753
Eco57I CTGAAG 1 cut(s) 160
Eco81I CCTNAGG 1 cut(s) 201
EcoNI CCTNNNNNAGG 1 cut(s) 200
FaeI CATG 5 cut(s) 40, 565, 583, 732, 769
FatI CATG 5 cut(s) 36, 561, 579, 728, 765
FauI CCCGC 1 cut(s) 269
Fnu4HI GCNGC 8 cut(s) 128, 131, 235, 238, 264, 615, 618, 726
FokI GGATG 3 cut(s) 43, 233, 292
Fsp4HI GCNGC 8 cut(s) 128, 131, 235, 238, 264, 615, 618, 726
FspBI CTAG 2 cut(s) 231, 317
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 1 cut(s) 444
GluI GCNGC 8 cut(s) 128, 131, 235, 238, 264, 615, 618, 726
HaeIII GGCC 4 cut(s) 543, 633, 707, 812
HapII CCGG 3 cut(s) 210, 602, 669
HgaI GACGC 1 cut(s) 282
HhaI GCGC 1 cut(s) 445
Hin1II CATG 5 cut(s) 40, 565, 583, 732, 769
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 7 cut(s) 120, 189, 268, 448, 467, 569, 683
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 3 cut(s) 210, 602, 669
HphI GGTGA 2 cut(s) 311, 608
Hpy166II GTNNAC 4 cut(s) 109, 314, 511, 787
Hpy188I TCNGA 3 cut(s) 146, 688, 757
Hpy188III TCNNGA 3 cut(s) 398, 533, 602
Hpy8I GTNNAC 4 cut(s) 109, 314, 511, 787
Hpy99I CGWCG 2 cut(s) 276, 761
HpyAV CCTTC 2 cut(s) 51, 57
HpyCH4III ACNGT 3 cut(s) 94, 106, 791
HpyCH4V TGCA 1 cut(s) 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 292, 379, 704, 776
HpyF3I CTNAG 3 cut(s) 201, 495, 687
Hsp92II CATG 5 cut(s) 40, 565, 583, 732, 769
HspAI GCGC 1 cut(s) 443
Kpn2I TCCGGA 1 cut(s) 601
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 3 cut(s) 346, 547, 598
Lsp1109I GCAGC 3 cut(s) 601, 604, 712
LweI GCATC 2 cut(s) 211, 222
MaeI CTAG 2 cut(s) 231, 317
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 348, 549, 600
MboI GATC 3 cut(s) 346, 547, 598
MboII GAAGA 5 cut(s) 166, 184, 198, 422, 703
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 2 cut(s) 346, 547
MluCI AATT 4 cut(s) 70, 323, 336, 802
MlyI GAGTC 3 cut(s) 129, 183, 476
MmeI TCCRAC 2 cut(s) 324, 780
MnlI CCTC 5 cut(s) 29, 85, 360, 457, 529
MroI TCCGGA 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 2 cut(s) 510, 585
MspI CCGG 3 cut(s) 210, 602, 669
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 140
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 5 cut(s) 263, 292, 379, 704, 776
NdeII GATC 3 cut(s) 346, 547, 598
NlaIII CATG 5 cut(s) 40, 565, 583, 732, 769
NlaIV GGNNCC 2 cut(s) 348, 706
NmeAIII GCCGAG 1 cut(s) 569
NsbI TGCGCA 1 cut(s) 444
NspI RCATGY 3 cut(s) 40, 583, 732
PaeI GCATGC 1 cut(s) 732
PciI ACATGT 1 cut(s) 579
PctI GAATGC 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 4 cut(s) 268, 448, 569, 683
PkrI GCNGC 8 cut(s) 129, 132, 236, 239, 265, 616, 619, 727
PleI GAGTC 3 cut(s) 128, 183, 475
PpsI GAGTC 3 cut(s) 128, 183, 475
PscI ACATGT 1 cut(s) 579
PshAI GACNNNNGTC 1 cut(s) 751
PspN4I GGNNCC 2 cut(s) 348, 706
PspPI GGNCC 2 cut(s) 705, 753
PsuI RGATCY 2 cut(s) 346, 547
SaqAI TTAA 2 cut(s) 510, 585
SatI GCNGC 8 cut(s) 128, 131, 235, 238, 264, 615, 618, 726
Sau3AI GATC 3 cut(s) 346, 547, 598
Sau96I GGNCC 2 cut(s) 705, 753
SchI GAGTC 3 cut(s) 129, 183, 476
SetI ASST 9 cut(s) 21, 207, 313, 407, 496, 540, 559, 612, 755
SfaNI GCATC 2 cut(s) 211, 222
SinI GGWCC 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
SphI GCATGC 1 cut(s) 732
Sse9I AATT 4 cut(s) 70, 323, 336, 802
SsiI CCGC 6 cut(s) 128, 131, 234, 237, 263, 276
SspMI CTAG 2 cut(s) 231, 317
TaaI ACNGT 3 cut(s) 94, 106, 791
TaqI TCGA 2 cut(s) 271, 799
TasI AATT 4 cut(s) 70, 323, 336, 802
TauI GCSGC 5 cut(s) 130, 133, 237, 240, 266
TfiI GAWTC 4 cut(s) 268, 448, 569, 683
Tru1I TTAA 2 cut(s) 510, 585
Tru9I TTAA 2 cut(s) 510, 585
TscAI CASTG 3 cut(s) 97, 160, 794
TseI GCWGC 3 cut(s) 614, 617, 725
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 2 cut(s) 40, 132
TspRI CASTG 3 cut(s) 97, 160, 794
VpaK11BI GGWCC 1 cut(s) 753
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 323
XceI RCATGY 3 cut(s) 40, 583, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 2 cut(s) 231, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.